gdh-1 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	gdh-1
Entrez Gene ID	3872927
Full Name	glutamate dehydrogenase-1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_951687.3	XP_956780.2	NAD-specific glutamate dehydrogenase

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	GDH2	NP_010066.1
Neurospora crassa	NCU00461	XP_956780.1
Kluyveromyces lactis	KLLA0D08481g	XP_453440.1
Schizosaccharomyces pombe (fission yeast)	gdh2	NP_588149.1
Magnaporthe oryzae (rice blast fungus)	MGG_05247	XP_003712804.1
Eremothecium gossypii	AGOS_AGL040C	NP_986626.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gdh-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gdh-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf10171	XM_951687.3 Latest version!	Neurospora crassa OR74A NAD-specific glutamate dehydrogenase (gdh-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gdh-1 ( XM_951687.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_956780.2 Neurospora crassa OR74A NAD-specific glutamate dehydrogenase (gdh-1), mRNA.

Price & Availability↑

CloneID	ONf10171
Clone ID Related Accession (Same CDS sequence)	XM_951687.3
Accession Version	XM_951687.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	NAD-specific glutamate dehydrogenase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503). On Feb 23, 2015 this sequence version replaced XM_951687.2.

ATGATGGATT CTCCTTCAGC CCCGGTCCCA GCCCACAAGT TGGTTGATCG CCTCAAAGAT 
CAGACGCCCA GACATCCTTC TCCTCAGCCG ACACATGTCA GCTATCCCAA GGTGAACGGC 
AACGGGCACA GGGTGCTCCG CTCCGCAACT GTTGGATATG TTGCCCCTGT CTTCCAGGGA 
AAGGCAGAGC AGATGAAGCA AGTCAAGAAT ATCATTGTCC AGGGCGGCTG GATTCCCGAG 
ACTCTGGTTG ACGGTCAGAT TGCGTGGTTC TATAATGAGC TTGGCATCGA CGACGTCTAC 
TTCCAACTAG AAAACCCTCA GGCGGTTGCA AATCATATCA CATCTCTCTA TGCGGCCAAA 
GTTGCTGCTT TCTCTCGCGA GGACAAGCGG GAAGAGATCA GACTGGATAT GGAAGCCTCG 
GACCACGCCA TTTACATCGA CACAAGCGAA CCCGGCATGA CCTCCTTCGA CGGTCCGCGA 
TATGAGCACA GACTCGAGTC CAAGTACTTG GATGGCGACG ATACTTCGAA GCGTTTCCGT 
GTCGAGACTT TCAGATCCCC CGGTGTCCTG GGGCAGAAGG AGAACTCCAA GGCAGCTCTT 
CGTTGCTACT TTGTCTATCA GTGTCTCTTT GTTGATTCTA ACGCCGATCC CAAGGAAACT 
CGCCTGGAAG TGATCAGCGA CCGCATGTTC CTTGCCAAGG CTACCAAGAA CACCAAGCAG 
ATCTACCAGG ACATCATCCA GGTTGCCGTG AGCCGACATG GTCCCGTCAT TGAGGTTTTC 
GACATCGAGG GCTCGGAGGA AATGAGACTG GTAGTTGCCT TCCGCTCTCG AACAGCAAAG 
GGTATCTTCA GCGCCCTCAG CGACCTTTAC CACTACTACG GCGTCACAAG CTCTCGTAAG 
TACGTTGAGC AGTTCTCCAA TGGTATTACC GTCATGAGTA TCTATCTGCG CCCTGCTGCG 
AACATCGACG GCAAGCACCC TCCTCTTGAG CAATCTATTC ACCAGATCAC CAAGGAGATT 
TCCTTGCTGT ACTGCCTTCC CCAGAACAAG TTTCACAACA TGTTTGCCTC TGGTGAACTC 
AGTCTGCAGG AGACCATCTA TGCCCACTGC GTCTGGGTGT TTGTTCAGCA TTTCTTGAAC 
CGTCTTGGGA CAGAGTACAC TTCGCTCATT GCAGCCCTGG ACCCCAAGAA CAACTCGCAT 
GTTGAGATTC TCTCCAAGAT GAAGAAGCGT CTGCGTACCG AGACCTTTAC ACCTGACTAC 
ATTCTTGAAA TCATCAGCTC CCACCCGCAA CTTGTGCGCG CTCTCTACGC CTCATTCGCC 
AGCGTTCACC TTCGGGTCGG CAGCGATTAT GATCGCCATC TCATTGCGCC TACGCCGGTC 
ATGGAGGTCC TTTCTGATGC CAGGCTTAAG GAGAAGATCA CCAAGGACGT TTCTAACGAG 
CACGAGGAAA TGGTTATGAC TGCCTTCCGT GTATTCAACA ATGCAGTCCT CAAGACGAAC 
TTCTTCACTC CCACCAAAGT CGCTTTGAGC TTCCGGCTTA ATCCCTCGTT CCTTCCGGAA 
GTCGAGTATC CCAAGCCGCT GTACGGAATG TTCCTCGTCA TCACTTCCGA GTCGCGAGGT 
TTCCACCTCC GCTTCAAGGA TATTGCACGG GGTGGTATTC GTATTGTCAA GTCTCGAAGT 
AAGGAGGCTT ACCAGATCAA TGCCCGCAAT CTCTTTGATG AGAACTACGG TCTTGCCAGC 
ACCCAGCAGC GCAAGAACAA GGATATTCCG GAGGGCGGAT CGAAGGGTGT CATTCTCCTT 
GACCCCAAGC AGCAAGACAG GCACCGCGAG GCCTTTGAGA AGTACATCGA TAGTATCCTT 
GACCTTCTTC TGAAGGCTGA GACGCCTGGC ATCAAGAACC CCATTGTCGA CCTCTACGGC 
AAGGAGGAGA TTTTGTTCAT GGGACCGGAT GAGAACACTG CTGACCTTGT CGACTGGGCC 
ACTGAGCATG CCCGGGCTCG CGGCGCTCCT TGGTGGAAAT CCTTCTTCAC TGGTAAATCA 
CCACGTCTTG GTGGCATCCC TCACGACTCC TATGGCATGA CAACCTTGTC GGTTCGTGAG 
TATGTCAAGG GTATTTACCG CAAGCTTGAG CTTGATCCCT CCAAGATCAG AAAGATGCAG 
ACTGGTGGTC CCGATGGTGA TCTGGGAAGC AACGAGATTC TGCTCAGCAA CGAGACTTAC 
ACTGCTATCG TTGACGGATC CGGTGTCCTC TGCGACCCCA ACGGCATTGA CAAGGATGAG 
TTACGCCGTC TTGCCAAGGC TCGTGCTATG ATCTCAAACT TTGATATCGC CAAGCTTTCC 
AAGGATGGTT ATCGTGTGCT TTGTGACGAT ACCAACGTCA CACTCCCCAA CGGAGAGGTT 
GTGCACAATG GCACTGCATT CCGTAACACG TACCATCTTC GCGACAACGG CATCACTGAT 
ATGTTCGTGC CTTGCGGTGG CCGTCCCGAG TCGATCGACC TCTCCTCAGT CAACAAGCTT 
ATCAAGGATG GAAAGTCAAC CATCCCATAT ATTGTTGAGG GTGCCAACCT GTTCATCACT 
CAAGATGCCA AGCTCAGACT GGAGGAAGCC GGCTGCATCG TTTACAAGGA TGCCAGTGCC 
AACAAAGGCG GTGTAACCAG CTCATCGCTC GAGGTCCTTG CCTCCCTCAG CTTTGACGAC 
AAGGGCTTCG TTACCCACAT GTGCCACGAT TCCCGCGGCA ATGCGCCCGA GTTCTACCAG 
GCCTATGTCA AGGAAGTGCA AAACAAGATC CAGGACAATG CCCGCCTCGA GTTCGAGGCC 
ATCTGGCGGG AACATGAGCA GACTGGCCTT CCTCGATCTG TTCTCTCTGA CAAGTTGTCA 
TTGGCCATCA CCTCTCTTGA CGAGGATCTC CAGCGTTCGG AGCTCTGGGA CAACGAGAAG 
ATTCGCAGGT CCGTCCTTGC CGATGCCTTG CCCAACCTTC TCATTAACAA GATCGGCCTC 
GATACCATCA TCGAGCGTGT TCCCGATTCG TATCTGAGGG CCATCTTTGG CAGCTACCTC 
GCCAGTCGCT TCGTCTATGA GTTTGGTAGC TCGCCCAGCC AGTTTGCTTT CTATGATTTC 
ATGTCAAAGA GAATGGGAAA CATCAATAAG GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_956780.2
CDS	967..4122
Translation	MMDSPSAPVPAHKLVDRLKDQTPRHPSPQPTHVSYPKVNGNGHRVLRSATVGYVAPVFQGKAEQMKQVKNIIVQGGWIPETLVDGQIAWFYNELGIDDVYFQLENPQAVANHITSLYAAKVAAFSREDKREEIRLDMEASDHAIYIDTSEPGMTSFDGPRYEHRLESKYLDGDDTSKRFRVETFRSPGVLGQKENSKAALRCYFVYQCLFVDSNADPKETRLEVISDRMFLAKATKNTKQIYQDIIQVAVSRHGPVIEVFDIEGSEEMRLVVAFRSRTAKGIFSALSDLYHYYGVTSSRKYVEQFSNGITVMSIYLRPAANIDGKHPPLEQSIHQITKEISLLYCLPQNKFHNMFASGELSLQETIYAHCVWVFVQHFLNRLGTEYTSLIAALDPKNNSHVEILSKMKKRLRTETFTPDYILEIISSHPQLVRALYASFASVHLRVGSDYDRHLIAPTPVMEVLSDARLKEKITKDVSNEHEEMVMTAFRVFNNAVLKTNFFTPTKVALSFRLNPSFLPEVEYPKPLYGMFLVITSESRGFHLRFKDIARGGIRIVKSRSKEAYQINARNLFDENYGLASTQQRKNKDIPEGGSKGVILLDPKQQDRHREAFEKYIDSILDLLLKAETPGIKNPIVDLYGKEEILFMGPDENTADLVDWATEHARARGAPWWKSFFTGKSPRLGGIPHDSYGMTTLSVREYVKGIYRKLELDPSKIRKMQTGGPDGDLGSNEILLSNETYTAIVDGSGVLCDPNGIDKDELRRLAKARAMISNFDIAKLSKDGYRVLCDDTNVTLPNGEVVHNGTAFRNTYHLRDNGITDMFVPCGGRPESIDLSSVNKLIKDGKSTIPYIVEGANLFITQDAKLRLEEAGCIVYKDASANKGGVTSSSLEVLASLSFDDKGFVTHMCHDSRGNAPEFYQAYVKEVQNKIQDNARLEFEAIWREHEQTGLPRSVLSDKLSLAITSLDEDLQRSELWDNEKIRRSVLADALPNLLINKIGLDTIIERVPDSYLRAIFGSYLASRFVYEFGSSPSQFAFYDFMSKRMGNINKE

Target ORF information:

RefSeq Version	XM_951687.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A NAD-specific glutamate dehydrogenase (gdh-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_951687.3

ORF Insert Sequence:

ATGATGGATT CTCCTTCAGC CCCGGTCCCA GCCCACAAGT TGGTTGATCG CCTCAAAGAT 
CAGACGCCCA GACATCCTTC TCCTCAGCCG ACACATGTCA GCTATCCCAA GGTGAACGGC 
AACGGGCACA GGGTGCTCCG CTCCGCAACT GTTGGATATG TTGCCCCTGT CTTCCAGGGA 
AAGGCAGAGC AGATGAAGCA AGTCAAGAAT ATCATTGTCC AGGGCGGCTG GATTCCCGAG 
ACTCTGGTTG ACGGTCAGAT TGCGTGGTTC TATAATGAGC TTGGCATCGA CGACGTCTAC 
TTCCAACTAG AAAACCCTCA GGCGGTTGCA AATCATATCA CATCTCTCTA TGCGGCCAAA 
GTTGCTGCTT TCTCTCGCGA GGACAAGCGG GAAGAGATCA GACTGGATAT GGAAGCCTCG 
GACCACGCCA TTTACATCGA CACAAGCGAA CCCGGCATGA CCTCCTTCGA CGGTCCGCGA 
TATGAGCACA GACTCGAGTC CAAGTACTTG GATGGCGACG ATACTTCGAA GCGTTTCCGT 
GTCGAGACTT TCAGATCCCC CGGTGTCCTG GGGCAGAAGG AGAACTCCAA GGCAGCTCTT 
CGTTGCTACT TTGTCTATCA GTGTCTCTTT GTTGATTCTA ACGCCGATCC CAAGGAAACT 
CGCCTGGAAG TGATCAGCGA CCGCATGTTC CTTGCCAAGG CTACCAAGAA CACCAAGCAG 
ATCTACCAGG ACATCATCCA GGTTGCCGTG AGCCGACATG GTCCCGTCAT TGAGGTTTTC 
GACATCGAGG GCTCGGAGGA AATGAGACTG GTAGTTGCCT TCCGCTCTCG AACAGCAAAG 
GGTATCTTCA GCGCCCTCAG CGACCTTTAC CACTACTACG GCGTCACAAG CTCTCGTAAG 
TACGTTGAGC AGTTCTCCAA TGGTATTACC GTCATGAGTA TCTATCTGCG CCCTGCTGCG 
AACATCGACG GCAAGCACCC TCCTCTTGAG CAATCTATTC ACCAGATCAC CAAGGAGATT 
TCCTTGCTGT ACTGCCTTCC CCAGAACAAG TTTCACAACA TGTTTGCCTC TGGTGAACTC 
AGTCTGCAGG AGACCATCTA TGCCCACTGC GTCTGGGTGT TTGTTCAGCA TTTCTTGAAC 
CGTCTTGGGA CAGAGTACAC TTCGCTCATT GCAGCCCTGG ACCCCAAGAA CAACTCGCAT 
GTTGAGATTC TCTCCAAGAT GAAGAAGCGT CTGCGTACCG AGACCTTTAC ACCTGACTAC 
ATTCTTGAAA TCATCAGCTC CCACCCGCAA CTTGTGCGCG CTCTCTACGC CTCATTCGCC 
AGCGTTCACC TTCGGGTCGG CAGCGATTAT GATCGCCATC TCATTGCGCC TACGCCGGTC 
ATGGAGGTCC TTTCTGATGC CAGGCTTAAG GAGAAGATCA CCAAGGACGT TTCTAACGAG 
CACGAGGAAA TGGTTATGAC TGCCTTCCGT GTATTCAACA ATGCAGTCCT CAAGACGAAC 
TTCTTCACTC CCACCAAAGT CGCTTTGAGC TTCCGGCTTA ATCCCTCGTT CCTTCCGGAA 
GTCGAGTATC CCAAGCCGCT GTACGGAATG TTCCTCGTCA TCACTTCCGA GTCGCGAGGT 
TTCCACCTCC GCTTCAAGGA TATTGCACGG GGTGGTATTC GTATTGTCAA GTCTCGAAGT 
AAGGAGGCTT ACCAGATCAA TGCCCGCAAT CTCTTTGATG AGAACTACGG TCTTGCCAGC 
ACCCAGCAGC GCAAGAACAA GGATATTCCG GAGGGCGGAT CGAAGGGTGT CATTCTCCTT 
GACCCCAAGC AGCAAGACAG GCACCGCGAG GCCTTTGAGA AGTACATCGA TAGTATCCTT 
GACCTTCTTC TGAAGGCTGA GACGCCTGGC ATCAAGAACC CCATTGTCGA CCTCTACGGC 
AAGGAGGAGA TTTTGTTCAT GGGACCGGAT GAGAACACTG CTGACCTTGT CGACTGGGCC 
ACTGAGCATG CCCGGGCTCG CGGCGCTCCT TGGTGGAAAT CCTTCTTCAC TGGTAAATCA 
CCACGTCTTG GTGGCATCCC TCACGACTCC TATGGCATGA CAACCTTGTC GGTTCGTGAG 
TATGTCAAGG GTATTTACCG CAAGCTTGAG CTTGATCCCT CCAAGATCAG AAAGATGCAG 
ACTGGTGGTC CCGATGGTGA TCTGGGAAGC AACGAGATTC TGCTCAGCAA CGAGACTTAC 
ACTGCTATCG TTGACGGATC CGGTGTCCTC TGCGACCCCA ACGGCATTGA CAAGGATGAG 
TTACGCCGTC TTGCCAAGGC TCGTGCTATG ATCTCAAACT TTGATATCGC CAAGCTTTCC 
AAGGATGGTT ATCGTGTGCT TTGTGACGAT ACCAACGTCA CACTCCCCAA CGGAGAGGTT 
GTGCACAATG GCACTGCATT CCGTAACACG TACCATCTTC GCGACAACGG CATCACTGAT 
ATGTTCGTGC CTTGCGGTGG CCGTCCCGAG TCGATCGACC TCTCCTCAGT CAACAAGCTT 
ATCAAGGATG GAAAGTCAAC CATCCCATAT ATTGTTGAGG GTGCCAACCT GTTCATCACT 
CAAGATGCCA AGCTCAGACT GGAGGAAGCC GGCTGCATCG TTTACAAGGA TGCCAGTGCC 
AACAAAGGCG GTGTAACCAG CTCATCGCTC GAGGTCCTTG CCTCCCTCAG CTTTGACGAC 
AAGGGCTTCG TTACCCACAT GTGCCACGAT TCCCGCGGCA ATGCGCCCGA GTTCTACCAG 
GCCTATGTCA AGGAAGTGCA AAACAAGATC CAGGACAATG CCCGCCTCGA GTTCGAGGCC 
ATCTGGCGGG AACATGAGCA GACTGGCCTT CCTCGATCTG TTCTCTCTGA CAAGTTGTCA 
TTGGCCATCA CCTCTCTTGA CGAGGATCTC CAGCGTTCGG AGCTCTGGGA CAACGAGAAG 
ATTCGCAGGT CCGTCCTTGC CGATGCCTTG CCCAACCTTC TCATTAACAA GATCGGCCTC 
GATACCATCA TCGAGCGTGT TCCCGATTCG TATCTGAGGG CCATCTTTGG CAGCTACCTC 
GCCAGTCGCT TCGTCTATGA GTTTGGTAGC TCGCCCAGCC AGTTTGCTTT CTATGATTTC 
ATGTCAAAGA GAATGGGAAA CATCAATAAG GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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gdh-1 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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