NCU03357 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU03357
Entrez Gene ID	3872604
Full Name	ubiquitin conjugation factor E4
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011395228.1	XP_011393530.1	ubiquitin conjugation factor E4, variant
XM_011395227.1	XP_011393529.1	ubiquitin conjugation factor E4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBE4B	XP_001161561.1
Kluyveromyces lactis	KLLA0F05269g	XP_455319.1
Eremothecium gossypii	AGOS_ADR096C	NP_984192.1
Neurospora crassa	NCU03357	XP_956466.1
Caenorhabditis elegans (roundworm)	ufd-2	NP_495691.1
Schizosaccharomyces pombe (fission yeast)	ufd2	NP_593630.1
Rattus norvegicus (Norway rat)	Ube4b	NP_001258127.1
Drosophila melanogaster (fruit fly)	CG9934	NP_609597.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007870	XP_317622.4
Magnaporthe oryzae (rice blast fungus)	MGG_03255	XP_003716719.1
Arabidopsis thaliana (thale cress)	AT5G15400	NP_568313.2
Mus musculus (house mouse)	Ube4b	NP_071305.2
Danio rerio (zebrafish)	ube4b	NP_919343.1
Xenopus tropicalis (tropical clawed frog)	ube4b	XP_004916084.1
Homo sapiens (human)	UBE4B	NP_001099032.1
	UBE4B	XP_001101836.1
Canis lupus familiaris (dog)	UBE4B	XP_005620583.1
Bos taurus (cattle)	UBE4B	NP_001095974.1
Gallus gallus (chicken)	UBE4B	XP_004947510.1
Saccharomyces cerevisiae (baker's yeast)	UFD2	NP_010091.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU03357 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU03357 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf10066	XM_011395227.1 Latest version!	Neurospora crassa OR74A ubiquitin conjugation factor E4 (NCU03357), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ONf10067	XM_011395228.1 Latest version!	Neurospora crassa OR74A ubiquitin conjugation factor E4, variant (NCU03357), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU03357 ( XM_011395227.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393529.1 Neurospora crassa OR74A ubiquitin conjugation factor E4 (NCU03357), partial mRNA.

Price & Availability↑

CloneID	ONf10066
Clone ID Related Accession (Same CDS sequence)	XM_011395227.1
Accession Version	XM_011395227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	ubiquitin conjugation factor E4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502). COMPLETENESS: incomplete on the 5' end.

ATGGATCCGA ATCAGCAACA GCCGGCGGCC ACGCCGGATG CGCCTGATAA AGAGACGATG 
GAGCAGATCC GTGCCCGACG ACTGGCTAAG CTCGGTGGCC CCAATGTCGC TTCTACTACA 
TCGCCAGCTG CGTCCTCTTC CACGCCTCAG CCTGGCACCG AAACCCCTCC CGCTGCTGCT 
GCTGTGGCGG CGGCGGCGGC GGCGGCGAGT TCGTCAAAGA TCGAAGACAA GGCACCGCGA 
GTTCAGCCAA GGTCGATAAT CAAGCCGGCG TCACGAGATG TGCCAGAGCC ACCACCAGGT 
AGTTCCGCTT CCCGAAAGAG AGAAAGATCC CTTTCAAATA TTGATGATAT TCCATGCCCA 
CCAGCACCGC GAAAGCAATA CAGGGAACAG ACGCTCGAGG AATGGACCGA CAAGCTACTC 
ACCAGCATCC TTCGCGTGTC TCTCGATCCG AACCAGACCT TCGATAGCAG TGGCCATAAC 
TTGACCTTTC TGCCAGAACT CAGCCAGGAA ATTCGGGGCG AGTATCAGGA GCCCTTGCTG 
TCGATCGACC GATTTCAAGA GGCCGTTATG GAGGCCGGAA GGATCTATCC GCCGCACAAG 
CCCCTATTCG AATACTTCCT GGCATGCTGG AAGCGGGTCA CCAGATATAG GATATTGAGG 
GCGTCGACTC CTGAGAAGGA AGAAGCCCTC AAGGAGGCCA AGCGCTTGTG CTTCTCGAAC 
TGCATCTTCG CCGTTACTAT GCCCGAGATG TTTGGTCGAG AACCAAACCC AAAGCACGAT 
ACCATAGTCC CCTACATTTT GGAAGGTGTT GCGAAGGAAG AAGGCCTCGA CATCGAGTTT 
TACAACGAGG CCATGACACG GATCGAGGAC GACGATAGCA TTGTACCTCT CTTCACCAAG 
GCCATGGTGG AAATCAGCAC AGCGCTGTCT ACCAAAAACA TGAACGGCGA TTATCAGCCG 
CACGTCCAGG CCCTCTTCAC ATACTCGAGA TATCCGGCTC TCGTGAACGC TTTGGCGGAA 
CACCCTACTT TCCTTATGGC GCAATCAGCC CCCAACATTG AACGGTTCAC GCTGCTCGGG 
CCATTCTTCC GCCTGTCGCC CTTACACCCA GAGGCGGCCA GTTACGACTT TGCCGCACCA 
AGGACTCTTG ACAAGGGCCG AATTGGCACA ACCCAACAAT CGTTACAAAT GACGTTGGCT 
GCCCATCAGG AACACTTGAC GACGATAGCT AATGCCTTCA TACGAGCAAG CACTAGCTCG 
AGAAACAAGC TCCTGGACTG GTTCGCCTAC ATCATGAATG TCAACCATAA GCGCACAGCC 
ACTTATGTCG ACCCCAAGAC CGTTTCGTCG GACGGCTTCA TGGTCAATGT CACTGTCGTC 
CTCGACAATC TATGCAAGCC TTTCATGGAT AACTCCTTTA CCAAGATTGA CCGTATTCAG 
GTCGACTACT TCAGGCGGAA GCCCAGATTG GACATCAAGG AAGAGACCAA GCTCAACGCT 
GACCAAGAGC ACTCGGACGC GTTTTACTCT ACAAAACTCG AAGGGGAGAA CAACTTTATA 
ACAGAGGTCT TCTTCCTCGC TTTGGCGGCA CATCAGTATG GCACCGAAGC CACCCAAAAC 
AAGCTAAAGG AACTTGACAA GCAAATTAAG CATTTCCAAA AGAACTTGAC CCTTATGGAG 
GCTGACCGTC CCAACATTGT CGCCAATCAT CCGGAACGAG TACCTATGCT AGAGGCGGCG 
CAGAGGAGAC TGATCAAGAT GCTGGAGAGC GCCATGTCGG CCAAATTTGC CATCGAAGGC 
ATTATGACCG ACAAGACGCT GCAAACGCGG TCACTACAGT TCATGAAGTA CACGATTGTG 
TGGCTACTGC GAGTAGCCAG TCAGTCCGAC TACGTCCCAT GGAAGAAAAT CAGCCTTCCG 
CTGCCTGAAA CTCAGCCAGA AGCTTTCAGG TGTCTTCCCG AGTATGTTCT GCAGGTAATT 
GTTGATAACT TGAAGTTCAC CTTCCGCCAT AGGCCAGAGG TCATGGTCTC GGCCATTGGT 
GACGAGGTCG TTGCTCTATG CATAACGTTT CTGGAATCCT CCGAGTACAT CAAGAACCCG 
TACTTGAAAT CGTCGCTGGT TACCCTCCTT TATCAGGGCA CCTGGCCGGC ATACCATCTT 
AAAAAGGGCA TTCTTGGCGA CATCCTTACC GGCACCAAGT TCGCGAATGA CTACCTGCTA 
CATGCTGTGA TGAAGTACTA TATTGAGTGC GAGTCGAATG GTACCAGTTC CGCGTTCTAC 
GAAAAGTTCA ACATACGTTT TGAGATCTTC CAGGTTATCA AGTGCGTCTG GACCAATGAC 
CATTACAAGA AGCAGTTGAC AGAGTCCAGC AGGGTGGACC GCGACTTCTT CGTTCGTTTT 
GTCAATCTTC TCATGAACGA TGCAACATAC GTTCTGGATG AAGCATTGAG CAACTTCCCC 
AAGATCCATG ACTTCCAGCA AAAGCTAAAG GACCCCAGTC TTTCGCAAGA GGATCGCGAA 
AAGATGGAGA GCGATTTACA CGACGCGGAA AACAAGGCCA GTTCCTATAT GCAGCTTGCG 
AACGAGACAG TCGGCATGAT GAAGCTCTTC ACCCAAACCC TGGCTGAGTC CTTCACCATG 
CCAGAAATCG TTCACCGCCT GGCCGGTATG CTCGATTTCA ACCTTGACCT CCTCACCGGT 
CCTAAGAGCC GGACGTTGAA GGTTGAGAAC CCCGACAAGT ATGGCTTCAA CCCCAAGATT 
CTGCTACCGC AGCTCGTGGA CATCTACCTG AACTTGGGTT CCTCGCCTGC CTTTGTCGAG 
GCCGTTGCTG CAGACGGCAG ATCCTACAAG CCTGAGACCA TGGCGTCTGC CACCAACATT 
CTTCGCTCAA AGGCACTCAA GAATCCTACC GAGATGCACG CGTGGGAGGT GTTGTGCAAA 
TGCTTCGAAG AGGCCAAGGC CATCGTCGAC CAGGCTGATC TCGACTTTGG CGACGCACCG 
CCCGAGTTCG AAGACCCCAT CATGGGCGAC TTGATGAAGG ACCCGGTGAT TCTACCCAGC 
AAGCACGTTG TCGATCGGTC GACTATCGTG CAGCACTTGC TCAGTGACCC GAAAGACCCG 
TTCACGAGAC AGCCCATGAC TATTGATGAT GTCATTCCGG ATACGGAACT GAAGGCTAAG 
ATTGAGAAGT GGATGGAGGA CAGGAGGGCA GAGATTCGCG GCAAGGTCGA GGAAGTGACG 
CCTGCTGTTG GTGATATTGC CGGAGCGGAG GCGGCGACGG GCGGAGATGC TATGGACACT 
ACCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393529.1
CDS	1..3309
Translation	MDPNQQQPAATPDAPDKETMEQIRARRLAKLGGPNVASTTSPAASSSTPQPGTETPPAAAAVAAAAAAASSSKIEDKAPRVQPRSIIKPASRDVPEPPPGSSASRKRERSLSNIDDIPCPPAPRKQYREQTLEEWTDKLLTSILRVSLDPNQTFDSSGHNLTFLPELSQEIRGEYQEPLLSIDRFQEAVMEAGRIYPPHKPLFEYFLACWKRVTRYRILRASTPEKEEALKEAKRLCFSNCIFAVTMPEMFGREPNPKHDTIVPYILEGVAKEEGLDIEFYNEAMTRIEDDDSIVPLFTKAMVEISTALSTKNMNGDYQPHVQALFTYSRYPALVNALAEHPTFLMAQSAPNIERFTLLGPFFRLSPLHPEAASYDFAAPRTLDKGRIGTTQQSLQMTLAAHQEHLTTIANAFIRASTSSRNKLLDWFAYIMNVNHKRTATYVDPKTVSSDGFMVNVTVVLDNLCKPFMDNSFTKIDRIQVDYFRRKPRLDIKEETKLNADQEHSDAFYSTKLEGENNFITEVFFLALAAHQYGTEATQNKLKELDKQIKHFQKNLTLMEADRPNIVANHPERVPMLEAAQRRLIKMLESAMSAKFAIEGIMTDKTLQTRSLQFMKYTIVWLLRVASQSDYVPWKKISLPLPETQPEAFRCLPEYVLQVIVDNLKFTFRHRPEVMVSAIGDEVVALCITFLESSEYIKNPYLKSSLVTLLYQGTWPAYHLKKGILGDILTGTKFANDYLLHAVMKYYIECESNGTSSAFYEKFNIRFEIFQVIKCVWTNDHYKKQLTESSRVDRDFFVRFVNLLMNDATYVLDEALSNFPKIHDFQQKLKDPSLSQEDREKMESDLHDAENKASSYMQLANETVGMMKLFTQTLAESFTMPEIVHRLAGMLDFNLDLLTGPKSRTLKVENPDKYGFNPKILLPQLVDIYLNLGSSPAFVEAVAADGRSYKPETMASATNILRSKALKNPTEMHAWEVLCKCFEEAKAIVDQADLDFGDAPPEFEDPIMGDLMKDPVILPSKHVVDRSTIVQHLLSDPKDPFTRQPMTIDDVIPDTELKAKIEKWMEDRRAEIRGKVEEVTPAVGDIAGAEAATGGDAMDTTE

Target ORF information:

RefSeq Version	XM_011395227.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A ubiquitin conjugation factor E4 (NCU03357), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395227.1

ORF Insert Sequence:

ATGGATCCGA ATCAGCAACA GCCGGCGGCC ACGCCGGATG CGCCTGATAA AGAGACGATG 
GAGCAGATCC GTGCCCGACG ACTGGCTAAG CTCGGTGGCC CCAATGTCGC TTCTACTACA 
TCGCCAGCTG CGTCCTCTTC CACGCCTCAG CCTGGCACCG AAACCCCTCC CGCTGCTGCT 
GCTGTGGCGG CGGCGGCGGC GGCGGCGAGT TCGTCAAAGA TCGAAGACAA GGCACCGCGA 
GTTCAGCCAA GGTCGATAAT CAAGCCGGCG TCACGAGATG TGCCAGAGCC ACCACCAGGT 
AGTTCCGCTT CCCGAAAGAG AGAAAGATCC CTTTCAAATA TTGATGATAT TCCATGCCCA 
CCAGCACCGC GAAAGCAATA CAGGGAACAG ACGCTCGAGG AATGGACCGA CAAGCTACTC 
ACCAGCATCC TTCGCGTGTC TCTCGATCCG AACCAGACCT TCGATAGCAG TGGCCATAAC 
TTGACCTTTC TGCCAGAACT CAGCCAGGAA ATTCGGGGCG AGTATCAGGA GCCCTTGCTG 
TCGATCGACC GATTTCAAGA GGCCGTTATG GAGGCCGGAA GGATCTATCC GCCGCACAAG 
CCCCTATTCG AATACTTCCT GGCATGCTGG AAGCGGGTCA CCAGATATAG GATATTGAGG 
GCGTCGACTC CTGAGAAGGA AGAAGCCCTC AAGGAGGCCA AGCGCTTGTG CTTCTCGAAC 
TGCATCTTCG CCGTTACTAT GCCCGAGATG TTTGGTCGAG AACCAAACCC AAAGCACGAT 
ACCATAGTCC CCTACATTTT GGAAGGTGTT GCGAAGGAAG AAGGCCTCGA CATCGAGTTT 
TACAACGAGG CCATGACACG GATCGAGGAC GACGATAGCA TTGTACCTCT CTTCACCAAG 
GCCATGGTGG AAATCAGCAC AGCGCTGTCT ACCAAAAACA TGAACGGCGA TTATCAGCCG 
CACGTCCAGG CCCTCTTCAC ATACTCGAGA TATCCGGCTC TCGTGAACGC TTTGGCGGAA 
CACCCTACTT TCCTTATGGC GCAATCAGCC CCCAACATTG AACGGTTCAC GCTGCTCGGG 
CCATTCTTCC GCCTGTCGCC CTTACACCCA GAGGCGGCCA GTTACGACTT TGCCGCACCA 
AGGACTCTTG ACAAGGGCCG AATTGGCACA ACCCAACAAT CGTTACAAAT GACGTTGGCT 
GCCCATCAGG AACACTTGAC GACGATAGCT AATGCCTTCA TACGAGCAAG CACTAGCTCG 
AGAAACAAGC TCCTGGACTG GTTCGCCTAC ATCATGAATG TCAACCATAA GCGCACAGCC 
ACTTATGTCG ACCCCAAGAC CGTTTCGTCG GACGGCTTCA TGGTCAATGT CACTGTCGTC 
CTCGACAATC TATGCAAGCC TTTCATGGAT AACTCCTTTA CCAAGATTGA CCGTATTCAG 
GTCGACTACT TCAGGCGGAA GCCCAGATTG GACATCAAGG AAGAGACCAA GCTCAACGCT 
GACCAAGAGC ACTCGGACGC GTTTTACTCT ACAAAACTCG AAGGGGAGAA CAACTTTATA 
ACAGAGGTCT TCTTCCTCGC TTTGGCGGCA CATCAGTATG GCACCGAAGC CACCCAAAAC 
AAGCTAAAGG AACTTGACAA GCAAATTAAG CATTTCCAAA AGAACTTGAC CCTTATGGAG 
GCTGACCGTC CCAACATTGT CGCCAATCAT CCGGAACGAG TACCTATGCT AGAGGCGGCG 
CAGAGGAGAC TGATCAAGAT GCTGGAGAGC GCCATGTCGG CCAAATTTGC CATCGAAGGC 
ATTATGACCG ACAAGACGCT GCAAACGCGG TCACTACAGT TCATGAAGTA CACGATTGTG 
TGGCTACTGC GAGTAGCCAG TCAGTCCGAC TACGTCCCAT GGAAGAAAAT CAGCCTTCCG 
CTGCCTGAAA CTCAGCCAGA AGCTTTCAGG TGTCTTCCCG AGTATGTTCT GCAGGTAATT 
GTTGATAACT TGAAGTTCAC CTTCCGCCAT AGGCCAGAGG TCATGGTCTC GGCCATTGGT 
GACGAGGTCG TTGCTCTATG CATAACGTTT CTGGAATCCT CCGAGTACAT CAAGAACCCG 
TACTTGAAAT CGTCGCTGGT TACCCTCCTT TATCAGGGCA CCTGGCCGGC ATACCATCTT 
AAAAAGGGCA TTCTTGGCGA CATCCTTACC GGCACCAAGT TCGCGAATGA CTACCTGCTA 
CATGCTGTGA TGAAGTACTA TATTGAGTGC GAGTCGAATG GTACCAGTTC CGCGTTCTAC 
GAAAAGTTCA ACATACGTTT TGAGATCTTC CAGGTTATCA AGTGCGTCTG GACCAATGAC 
CATTACAAGA AGCAGTTGAC AGAGTCCAGC AGGGTGGACC GCGACTTCTT CGTTCGTTTT 
GTCAATCTTC TCATGAACGA TGCAACATAC GTTCTGGATG AAGCATTGAG CAACTTCCCC 
AAGATCCATG ACTTCCAGCA AAAGCTAAAG GACCCCAGTC TTTCGCAAGA GGATCGCGAA 
AAGATGGAGA GCGATTTACA CGACGCGGAA AACAAGGCCA GTTCCTATAT GCAGCTTGCG 
AACGAGACAG TCGGCATGAT GAAGCTCTTC ACCCAAACCC TGGCTGAGTC CTTCACCATG 
CCAGAAATCG TTCACCGCCT GGCCGGTATG CTCGATTTCA ACCTTGACCT CCTCACCGGT 
CCTAAGAGCC GGACGTTGAA GGTTGAGAAC CCCGACAAGT ATGGCTTCAA CCCCAAGATT 
CTGCTACCGC AGCTCGTGGA CATCTACCTG AACTTGGGTT CCTCGCCTGC CTTTGTCGAG 
GCCGTTGCTG CAGACGGCAG ATCCTACAAG CCTGAGACCA TGGCGTCTGC CACCAACATT 
CTTCGCTCAA AGGCACTCAA GAATCCTACC GAGATGCACG CGTGGGAGGT GTTGTGCAAA 
TGCTTCGAAG AGGCCAAGGC CATCGTCGAC CAGGCTGATC TCGACTTTGG CGACGCACCG 
CCCGAGTTCG AAGACCCCAT CATGGGCGAC TTGATGAAGG ACCCGGTGAT TCTACCCAGC 
AAGCACGTTG TCGATCGGTC GACTATCGTG CAGCACTTGC TCAGTGACCC GAAAGACCCG 
TTCACGAGAC AGCCCATGAC TATTGATGAT GTCATTCCGG ATACGGAACT GAAGGCTAAG 
ATTGAGAAGT GGATGGAGGA CAGGAGGGCA GAGATTCGCG GCAAGGTCGA GGAAGTGACG 
CCTGCTGTTG GTGATATTGC CGGAGCGGAG GCGGCGACGG GCGGAGATGC TATGGACACT 
ACCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU03357 ( XM_011395228.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393530.1 Neurospora crassa OR74A ubiquitin conjugation factor E4, variant (NCU03357), mRNA.

Price & Availability↑

CloneID	ONf10067
Clone ID Related Accession (Same CDS sequence)	XM_011395228.1
Accession Version	XM_011395228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	ubiquitin conjugation factor E4, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502).

ATGGATCCGA ATCAGCAACA GCCGGCGGCC ACGCCGGATG CGCCTGATAA AGAGACGATG 
GAGCAGATCC GTGCCCGACG ACTGGCTAAG CTCGGTGGCC CCAATGTCGC TTCTACTACA 
TCGCCAGCTG CGTCCTCTTC CACGCCTCAG CCTGGCACCG AAACCCCTCC CGCTGCTGCT 
GCTGTGGCGG CGGCGGCGGC GGCGGCGAGT TCGTCAAAGA TCGAAGACAA GGCACCGCGA 
GTTCAGCCAA GGTCGATAAT CAAGCCGGCG TCACGAGATG TGCCAGAGCC ACCACCAGCA 
CCGCGAAAGC AATACAGGGA ACAGACGCTC GAGGAATGGA CCGACAAGCT ACTCACCAGC 
ATCCTTCGCG TGTCTCTCGA TCCGAACCAG ACCTTCGATA GCAGTGGCCA TAACTTGACC 
TTTCTGCCAG AACTCAGCCA GGAAATTCGG GGCGAGTATC AGGAGCCCTT GCTGTCGATC 
GACCGATTTC AAGAGGCCGT TATGGAGGCC GGAAGGATCT ATCCGCCGCA CAAGCCCCTA 
TTCGAATACT TCCTGGCATG CTGGAAGCGG GTCACCAGAT ATAGGATATT GAGGGCGTCG 
ACTCCTGAGA AGGAAGAAGC CCTCAAGGAG GCCAAGCGCT TGTGCTTCTC GAACTGCATC 
TTCGCCGTTA CTATGCCCGA GATGTTTGGT CGAGAACCAA ACCCAAAGCA CGATACCATA 
GTCCCCTACA TTTTGGAAGG TGTTGCGAAG GAAGAAGGCC TCGACATCGA GTTTTACAAC 
GAGGCCATGA CACGGATCGA GGACGACGAT AGCATTGTAC CTCTCTTCAC CAAGGCCATG 
GTGGAAATCA GCACAGCGCT GTCTACCAAA AACATGAACG GCGATTATCA GCCGCACGTC 
CAGGCCCTCT TCACATACTC GAGATATCCG GCTCTCGTGA ACGCTTTGGC GGAACACCCT 
ACTTTCCTTA TGGCGCAATC AGCCCCCAAC ATTGAACGGT TCACGCTGCT CGGGCCATTC 
TTCCGCCTGT CGCCCTTACA CCCAGAGGCG GCCAGTTACG ACTTTGCCGC ACCAAGGACT 
CTTGACAAGG GCCGAATTGG CACAACCCAA CAATCGTTAC AAATGACGTT GGCTGCCCAT 
CAGGAACACT TGACGACGAT AGCTAATGCC TTCATACGAG CAAGCACTAG CTCGAGAAAC 
AAGCTCCTGG ACTGGTTCGC CTACATCATG AATGTCAACC ATAAGCGCAC AGCCACTTAT 
GTCGACCCCA AGACCGTTTC GTCGGACGGC TTCATGGTCA ATGTCACTGT CGTCCTCGAC 
AATCTATGCA AGCCTTTCAT GGATAACTCC TTTACCAAGA TTGACCGTAT TCAGGTCGAC 
TACTTCAGGC GGAAGCCCAG ATTGGACATC AAGGAAGAGA CCAAGCTCAA CGCTGACCAA 
GAGCACTCGG ACGCGTTTTA CTCTACAAAA CTCGAAGGGG AGAACAACTT TATAACAGAG 
GTCTTCTTCC TCGCTTTGGC GGCACATCAG TATGGCACCG AAGCCACCCA AAACAAGCTA 
AAGGAACTTG ACAAGCAAAT TAAGCATTTC CAAAAGAACT TGACCCTTAT GGAGGCTGAC 
CGTCCCAACA TTGTCGCCAA TCATCCGGAA CGAGTACCTA TGCTAGAGGC GGCGCAGAGG 
AGACTGATCA AGATGCTGGA GAGCGCCATG TCGGCCAAAT TTGCCATCGA AGGCATTATG 
ACCGACAAGA CGCTGCAAAC GCGGTCACTA CAGTTCATGA AGTACACGAT TGTGTGGCTA 
CTGCGAGTAG CCAGTCAGTC CGACTACGTC CCATGGAAGA AAATCAGCCT TCCGCTGCCT 
GAAACTCAGC CAGAAGCTTT CAGGTGTCTT CCCGAGTATG TTCTGCAGGT AATTGTTGAT 
AACTTGAAGT TCACCTTCCG CCATAGGCCA GAGGTCATGG TCTCGGCCAT TGGTGACGAG 
GTCGTTGCTC TATGCATAAC GTTTCTGGAA TCCTCCGAGT ACATCAAGAA CCCGTACTTG 
AAATCGTCGC TGGTTACCCT CCTTTATCAG GGCACCTGGC CGGCATACCA TCTTAAAAAG 
GGCATTCTTG GCGACATCCT TACCGGCACC AAGTTCGCGA ATGACTACCT GCTACATGCT 
GTGATGAAGT ACTATATTGA GTGCGAGTCG AATGGTACCA GTTCCGCGTT CTACGAAAAG 
TTCAACATAC GTTTTGAGAT CTTCCAGGTT ATCAAGTGCG TCTGGACCAA TGACCATTAC 
AAGAAGCAGT TGACAGAGTC CAGCAGGGTG GACCGCGACT TCTTCGTTCG TTTTGTCAAT 
CTTCTCATGA ACGATGCAAC ATACGTTCTG GATGAAGCAT TGAGCAACTT CCCCAAGATC 
CATGACTTCC AGCAAAAGCT AAAGGACCCC AGTCTTTCGC AAGAGGATCG CGAAAAGATG 
GAGAGCGATT TACACGACGC GGAAAACAAG GCCAGTTCCT ATATGCAGCT TGCGAACGAG 
ACAGTCGGCA TGATGAAGCT CTTCACCCAA ACCCTGGCTG AGTCCTTCAC CATGCCAGAA 
ATCGTTCACC GCCTGGCCGG TATGCTCGAT TTCAACCTTG ACCTCCTCAC CGGTCCTAAG 
AGCCGGACGT TGAAGGTTGA GAACCCCGAC AAGTATGGCT TCAACCCCAA GATTCTGCTA 
CCGCAGCTCG TGGACATCTA CCTGAACTTG GGTTCCTCGC CTGCCTTTGT CGAGGCCGTT 
GCTGCAGACG GCAGATCCTA CAAGCCTGAG ACCATGGCGT CTGCCACCAA CATTCTTCGC 
TCAAAGGCAC TCAAGAATCC TACCGAGATG CACGCGTGGG AGGTGTTGTG CAAATGCTTC 
GAAGAGGCCA AGGCCATCGT CGACCAGGCT GATCTCGACT TTGGCGACGC ACCGCCCGAG 
TTCGAAGACC CCATCATGGG CGACTTGATG AAGGACCCGG TGATTCTACC CAGCAAGCAC 
GTTGTCGATC GGTCGACTAT CGTGCAGCAC TTGCTCAGTG ACCCGAAAGA CCCGTTCACG 
AGACAGCCCA TGACTATTGA TGATGTCATT CCGGATACGG AACTGAAGGC TAAGATTGAG 
AAGTGGATGG AGGACAGGAG GGCAGAGATT CGCGGCAAGG TCGAGGAAGT GACGCCTGCT 
GTTGGTGATA TTGCCGGAGC GGAGGCGGCG ACGGGCGGAG ATGCTATGGA CACTACCGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393530.1
CDS	360..3602
Translation	MDPNQQQPAATPDAPDKETMEQIRARRLAKLGGPNVASTTSPAASSSTPQPGTETPPAAAAVAAAAAAASSSKIEDKAPRVQPRSIIKPASRDVPEPPPAPRKQYREQTLEEWTDKLLTSILRVSLDPNQTFDSSGHNLTFLPELSQEIRGEYQEPLLSIDRFQEAVMEAGRIYPPHKPLFEYFLACWKRVTRYRILRASTPEKEEALKEAKRLCFSNCIFAVTMPEMFGREPNPKHDTIVPYILEGVAKEEGLDIEFYNEAMTRIEDDDSIVPLFTKAMVEISTALSTKNMNGDYQPHVQALFTYSRYPALVNALAEHPTFLMAQSAPNIERFTLLGPFFRLSPLHPEAASYDFAAPRTLDKGRIGTTQQSLQMTLAAHQEHLTTIANAFIRASTSSRNKLLDWFAYIMNVNHKRTATYVDPKTVSSDGFMVNVTVVLDNLCKPFMDNSFTKIDRIQVDYFRRKPRLDIKEETKLNADQEHSDAFYSTKLEGENNFITEVFFLALAAHQYGTEATQNKLKELDKQIKHFQKNLTLMEADRPNIVANHPERVPMLEAAQRRLIKMLESAMSAKFAIEGIMTDKTLQTRSLQFMKYTIVWLLRVASQSDYVPWKKISLPLPETQPEAFRCLPEYVLQVIVDNLKFTFRHRPEVMVSAIGDEVVALCITFLESSEYIKNPYLKSSLVTLLYQGTWPAYHLKKGILGDILTGTKFANDYLLHAVMKYYIECESNGTSSAFYEKFNIRFEIFQVIKCVWTNDHYKKQLTESSRVDRDFFVRFVNLLMNDATYVLDEALSNFPKIHDFQQKLKDPSLSQEDREKMESDLHDAENKASSYMQLANETVGMMKLFTQTLAESFTMPEIVHRLAGMLDFNLDLLTGPKSRTLKVENPDKYGFNPKILLPQLVDIYLNLGSSPAFVEAVAADGRSYKPETMASATNILRSKALKNPTEMHAWEVLCKCFEEAKAIVDQADLDFGDAPPEFEDPIMGDLMKDPVILPSKHVVDRSTIVQHLLSDPKDPFTRQPMTIDDVIPDTELKAKIEKWMEDRRAEIRGKVEEVTPAVGDIAGAEAATGGDAMDTTE

Target ORF information:

RefSeq Version	XM_011395228.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A ubiquitin conjugation factor E4, variant (NCU03357), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395228.1

ORF Insert Sequence:

ATGGATCCGA ATCAGCAACA GCCGGCGGCC ACGCCGGATG CGCCTGATAA AGAGACGATG 
GAGCAGATCC GTGCCCGACG ACTGGCTAAG CTCGGTGGCC CCAATGTCGC TTCTACTACA 
TCGCCAGCTG CGTCCTCTTC CACGCCTCAG CCTGGCACCG AAACCCCTCC CGCTGCTGCT 
GCTGTGGCGG CGGCGGCGGC GGCGGCGAGT TCGTCAAAGA TCGAAGACAA GGCACCGCGA 
GTTCAGCCAA GGTCGATAAT CAAGCCGGCG TCACGAGATG TGCCAGAGCC ACCACCAGCA 
CCGCGAAAGC AATACAGGGA ACAGACGCTC GAGGAATGGA CCGACAAGCT ACTCACCAGC 
ATCCTTCGCG TGTCTCTCGA TCCGAACCAG ACCTTCGATA GCAGTGGCCA TAACTTGACC 
TTTCTGCCAG AACTCAGCCA GGAAATTCGG GGCGAGTATC AGGAGCCCTT GCTGTCGATC 
GACCGATTTC AAGAGGCCGT TATGGAGGCC GGAAGGATCT ATCCGCCGCA CAAGCCCCTA 
TTCGAATACT TCCTGGCATG CTGGAAGCGG GTCACCAGAT ATAGGATATT GAGGGCGTCG 
ACTCCTGAGA AGGAAGAAGC CCTCAAGGAG GCCAAGCGCT TGTGCTTCTC GAACTGCATC 
TTCGCCGTTA CTATGCCCGA GATGTTTGGT CGAGAACCAA ACCCAAAGCA CGATACCATA 
GTCCCCTACA TTTTGGAAGG TGTTGCGAAG GAAGAAGGCC TCGACATCGA GTTTTACAAC 
GAGGCCATGA CACGGATCGA GGACGACGAT AGCATTGTAC CTCTCTTCAC CAAGGCCATG 
GTGGAAATCA GCACAGCGCT GTCTACCAAA AACATGAACG GCGATTATCA GCCGCACGTC 
CAGGCCCTCT TCACATACTC GAGATATCCG GCTCTCGTGA ACGCTTTGGC GGAACACCCT 
ACTTTCCTTA TGGCGCAATC AGCCCCCAAC ATTGAACGGT TCACGCTGCT CGGGCCATTC 
TTCCGCCTGT CGCCCTTACA CCCAGAGGCG GCCAGTTACG ACTTTGCCGC ACCAAGGACT 
CTTGACAAGG GCCGAATTGG CACAACCCAA CAATCGTTAC AAATGACGTT GGCTGCCCAT 
CAGGAACACT TGACGACGAT AGCTAATGCC TTCATACGAG CAAGCACTAG CTCGAGAAAC 
AAGCTCCTGG ACTGGTTCGC CTACATCATG AATGTCAACC ATAAGCGCAC AGCCACTTAT 
GTCGACCCCA AGACCGTTTC GTCGGACGGC TTCATGGTCA ATGTCACTGT CGTCCTCGAC 
AATCTATGCA AGCCTTTCAT GGATAACTCC TTTACCAAGA TTGACCGTAT TCAGGTCGAC 
TACTTCAGGC GGAAGCCCAG ATTGGACATC AAGGAAGAGA CCAAGCTCAA CGCTGACCAA 
GAGCACTCGG ACGCGTTTTA CTCTACAAAA CTCGAAGGGG AGAACAACTT TATAACAGAG 
GTCTTCTTCC TCGCTTTGGC GGCACATCAG TATGGCACCG AAGCCACCCA AAACAAGCTA 
AAGGAACTTG ACAAGCAAAT TAAGCATTTC CAAAAGAACT TGACCCTTAT GGAGGCTGAC 
CGTCCCAACA TTGTCGCCAA TCATCCGGAA CGAGTACCTA TGCTAGAGGC GGCGCAGAGG 
AGACTGATCA AGATGCTGGA GAGCGCCATG TCGGCCAAAT TTGCCATCGA AGGCATTATG 
ACCGACAAGA CGCTGCAAAC GCGGTCACTA CAGTTCATGA AGTACACGAT TGTGTGGCTA 
CTGCGAGTAG CCAGTCAGTC CGACTACGTC CCATGGAAGA AAATCAGCCT TCCGCTGCCT 
GAAACTCAGC CAGAAGCTTT CAGGTGTCTT CCCGAGTATG TTCTGCAGGT AATTGTTGAT 
AACTTGAAGT TCACCTTCCG CCATAGGCCA GAGGTCATGG TCTCGGCCAT TGGTGACGAG 
GTCGTTGCTC TATGCATAAC GTTTCTGGAA TCCTCCGAGT ACATCAAGAA CCCGTACTTG 
AAATCGTCGC TGGTTACCCT CCTTTATCAG GGCACCTGGC CGGCATACCA TCTTAAAAAG 
GGCATTCTTG GCGACATCCT TACCGGCACC AAGTTCGCGA ATGACTACCT GCTACATGCT 
GTGATGAAGT ACTATATTGA GTGCGAGTCG AATGGTACCA GTTCCGCGTT CTACGAAAAG 
TTCAACATAC GTTTTGAGAT CTTCCAGGTT ATCAAGTGCG TCTGGACCAA TGACCATTAC 
AAGAAGCAGT TGACAGAGTC CAGCAGGGTG GACCGCGACT TCTTCGTTCG TTTTGTCAAT 
CTTCTCATGA ACGATGCAAC ATACGTTCTG GATGAAGCAT TGAGCAACTT CCCCAAGATC 
CATGACTTCC AGCAAAAGCT AAAGGACCCC AGTCTTTCGC AAGAGGATCG CGAAAAGATG 
GAGAGCGATT TACACGACGC GGAAAACAAG GCCAGTTCCT ATATGCAGCT TGCGAACGAG 
ACAGTCGGCA TGATGAAGCT CTTCACCCAA ACCCTGGCTG AGTCCTTCAC CATGCCAGAA 
ATCGTTCACC GCCTGGCCGG TATGCTCGAT TTCAACCTTG ACCTCCTCAC CGGTCCTAAG 
AGCCGGACGT TGAAGGTTGA GAACCCCGAC AAGTATGGCT TCAACCCCAA GATTCTGCTA 
CCGCAGCTCG TGGACATCTA CCTGAACTTG GGTTCCTCGC CTGCCTTTGT CGAGGCCGTT 
GCTGCAGACG GCAGATCCTA CAAGCCTGAG ACCATGGCGT CTGCCACCAA CATTCTTCGC 
TCAAAGGCAC TCAAGAATCC TACCGAGATG CACGCGTGGG AGGTGTTGTG CAAATGCTTC 
GAAGAGGCCA AGGCCATCGT CGACCAGGCT GATCTCGACT TTGGCGACGC ACCGCCCGAG 
TTCGAAGACC CCATCATGGG CGACTTGATG AAGGACCCGG TGATTCTACC CAGCAAGCAC 
GTTGTCGATC GGTCGACTAT CGTGCAGCAC TTGCTCAGTG ACCCGAAAGA CCCGTTCACG 
AGACAGCCCA TGACTATTGA TGATGTCATT CCGGATACGG AACTGAAGGC TAAGATTGAG 
AAGTGGATGG AGGACAGGAG GGCAGAGATT CGCGGCAAGG TCGAGGAAGT GACGCCTGCT 
GTTGGTGATA TTGCCGGAGC GGAGGCGGCG ACGGGCGGAG ATGCTATGGA CACTACCGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU03357 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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