NCU01638 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU01638
Entrez Gene ID	3872153
Full Name	DNA-directed RNA polymerase I subunit RPA1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_950913.3	XP_956006.2	DNA-directed RNA polymerase I subunit RPA1

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLR1A	NP_056240.2
Pan troglodytes (chimpanzee)	POLR1A	XP_001135380.2
Caenorhabditis elegans (roundworm)	Y48E1A.1	NP_001022450.1
Eremothecium gossypii	AGOS_ADR374C	NP_984470.2
Schizosaccharomyces pombe (fission yeast)	nuc1	NP_596300.1
Saccharomyces cerevisiae (baker's yeast)	RPA190	NP_014986.3
Magnaporthe oryzae (rice blast fungus)	MGG_01158	XP_003717763.1
	POLR1A	XP_002808090.1
Gallus gallus (chicken)	POLR1A	XP_420851.3
Danio rerio (zebrafish)	polr1a	XP_001922839.2
Drosophila melanogaster (fruit fly)	RpI1	NP_523743.1
Xenopus tropicalis (tropical clawed frog)	polr1a	XP_002937328.2
Bos taurus (cattle)	POLR1A	NP_001179805.1
Rattus norvegicus (Norway rat)	Polr1a	NP_113960.1
Neurospora crassa	NCU01638	XP_956006.2
Arabidopsis thaliana (thale cress)	NRPA1	NP_191325.1
Canis lupus familiaris (dog)	POLR1A	XP_532976.3
Mus musculus (house mouse)	Polr1a	NP_033114.3
Kluyveromyces lactis	KLLA0F23243g	XP_456115.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU01638 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU01638 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf05125	XM_950913.3 Latest version!	Neurospora crassa OR74A DNA-directed RNA polymerase I subunit RPA1 (NCU01638), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU01638 ( XM_950913.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_956006.2 Neurospora crassa OR74A DNA-directed RNA polymerase I subunit RPA1 (NCU01638), mRNA.

Price & Availability↑

CloneID	ONf05125
Clone ID Related Accession (Same CDS sequence)	XM_950913.3
Accession Version	XM_950913.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	DNA-directed RNA polymerase I subunit RPA1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502). On Feb 23, 2015 this sequence version replaced XM_950913.2.

ATGAATATTT CGCAGCCCGT GTCGTCCTCT CTGGACAGCG TCGAGTTCAC CTTCCTGACC 
GACACCGAGA TCCGCGCCGT CTCGGTCCGC AAGATCGAGA ATGAGAGCAC CTTCGACAGC 
CTGCTGAACC CCGTCCCCGG CGGTCTCTAC GACCCTGCCT TGGGTTCCTG GGGCGACTCA 
CTCTGCACAA CATGTAACCT TGGCCAGTCG CAATGTCCCG GTCATCCCGG CCACATCGAG 
CTCCCCGTGC CCGTCTATCA CCCCGTCTTC ATGGACCAGG CATACCGTCT GCTGCGCGCG 
CAATGTGTTT ACTGCCACCG GTTCCGCCTT CCCAGGAGGG AAATCCACCG CTACTCCTGC 
ATGCTCCGTC TGCTGCAGTG TGGCCTTCTC AAGGAGGCCC AGATGATCGA CTCTTTCGGC 
GAAAGCGAGT TCGGCTCCGC CATCCGCCAA TTCCAGCTGG CCGACGTGCC CGAGATGGAG 
GATGACGAGG CTGAGGAGGA GGGAAACAAC TTGAACGACA GCACCATCCG CCAACGGGAA 
GCATATGTCC GCGCTGTCCT TCGCGATTAT AGGACTAAGG TGACTATGCG CGACATCAAC 
AAGGGCAAGC ACGAAGGCGC CGCCGATATG CGCAGGGCTC TTGTCAAGGA GTTCCTGGCT 
GCCATCATCA AGGACAAGAA GTGCAGGACT TGCGACGGCA TCTCTCCCGT CTACCGCAAG 
GATCGGTACA CCAAGATTTT CGAGCGCGAC CTCACACAAA AGGAGAAGGC CGCCATGGCC 
CAGGCTGGAA GGAAGCGTGC TGACGCGCTG GCCATGAAGG GCGGCAAGAA GAAGAATCAT 
GATGATGATG AGGGGATCGC CGATATTGAG TCGACAGCCA GCGAATCCGA CAACAGTGAA 
GGAGAGGGAG AAGAGCTCGA CGAGAACGGC GATGTTGTCA TGGCGGACGC GGGCAGCAAG 
ACCAAGGCGA AGGAGAAGGC CGAGAAGAGG GCCCTTCCCC AGAGATACAT CAGCTCTCTC 
GAGGTCAAGG AGAGGTTGAA CTTCCTCTTC CAGAAGGAGC AAGAGATTGT CTCTTTGCTC 
TTTAGCTCCA AGCCTCGTCC CAAGCACGCG AAGCCGATGA CCGCCGACAT GTTCTTCATC 
CAGACACTTC TCGTCCCTCC CAACAAATTC AGACCCGAGG CTAGAATGGG CGACCAAGTA 
ACGGAAGCGC AGCAAAACAG TCTCTACAAG CTAATCCTCA GGGGGTCGTC TATGGTCGCC 
CAGATCTCTC GCGAGGTTTC CGAAACCGCC AAGGGTAATG CCCCCGAGGA CGGACGCAGG 
GCCAGGGATA TCAATGCGCT CTACCAGGCG TGGACACAGC TTCAAGACGC CGTCAACTCC 
TTGATCGATC GCGATAAGAA CCCCGTCCAG GGTGCTGCTG GAAAGCGCAA CGAGGACGGT 
ATCAAGCAGA AGCTCGAGAA GAAGGAGGGT CTTTTCCGCA AGAACATGAT GGGTAAGCGT 
GTCAACTTTG CCGCCAGATC CGTCATCTCT CCCGATCCCA ACATCGAGAC CAGCGAGATC 
GGTGTCCCTC CTGTGTTCGC CAGGAAGCTC ACATACCCCG AGCCCGTAAC GAGCCACAAC 
TACCATGATC TCAAGAATGC AGTGCAGAAC GGTATGGAGA AGTGGCCCGG TGCTTCCGCC 
ATTGAGATGG AGAACGGCCA AATCATCAAC TTGAGGAACA AGTCTTCGGA GGAGCGTCAG 
GCCCTTGCCA ACCAGCTCTT GGCCCCGACC AACAACAACT TCAGCGGAGT TCGTAACAAG 
AAGGTCCACC GCCATCTTAC CAACGGTGAT GTCGTCCTCA TGAACCGTCA GCCCACCCTC 
CATAAGCCCT CCATCATGGG TCACAGAGTC AGAGTGCTGC CGGGCGAGAA GACGATTCGC 
ATGCACTACG CCAACTGCAA TACCTACAAC GCCGATTTCG ATGGTGATGA GATGAACATG 
CATTTCCCCC AAAACGAGAT TGCCCGTGCC GAGGCCCTTC AGCTCGCCGA CACTGATCAC 
CAATACATCT CCGGCACCGC TGGCAAGCCT CTCCGCGGTC TCATTCAGGA TCATTTGTCC 
GTTTCCGTTA TTCTGTGTAA CAAGGACACC TTCTTCGATC GGGCCAACTA CCATCAACTC 
ATCTACGCCG CTCTTCGGCC CGAAAGTGGT CACATTATGG GAGACAAGAT TGAGCTTTTG 
CCGCCGGCGA TCATCAAGCC GGTTCCCCGC TGGACGGGTA AGCAAGTCAT TACCACAATC 
CTCAAGAACA TCAAGCCCCC AAATTGCGGT GATTTGTGGA TGAACGGCTC TACACAAGTC 
AAGGCTCATC AGTGGGGTGA CCATGCCCCA GAAGAAGGCC AGGTCACTTT CCGTGATGGA 
GAGTTCATCA CCGGTATCCT CGACAAGTCT CAGCTGGGTC CCAGCAGTGG TGGTTTGATT 
CACTCGATTC ACGAAGTTTA CGGCCCTTCA GCTGCTGGTA AGCTCCTCAG TTGCATGGGT 
CGTCTCCTCA CTCGTTACCT GGCCATGGTT GCCTTTTCGT GCGGTATGGA CGATCTAGTC 
ATGACGCCCA AGGGAGAGGC AGACAGAAAG GAGAAGATCA AGGCCGCCGC TCACATCGGT 
CTCGAGGTCG CCGCCAAGTA TGTGTCGCTC GAGGAGCAGA AGCCTACTCC CGAAGATCCC 
CTTCTCTTGC AACGCTTGGA GGAAGTTATG CGCAACGACA AGAAGCAAGA GAACTTGGAT 
CTCTTGATGA ACCAACGCTG CGCCCAACTT TCTAGCGAGA TCACCAAGAC ATGTCTTCCT 
GCTGGTCTGC AAAAGAAGTT CCCCAAGAAC CAAATGCAAA GTATGACAAC ATCAGGTGCT 
AAGGGTTCTC CTGTCAACGC CAACCTCATC TCTTGTAACT TGGGTCAGCA GGTTTTGGAA 
GGTAGACGTG TCCCCGTCAT GGTCAGCGGC AAGACTCTCC CTAGTTTCAA GCCCTTCGAT 
ACCAACGCCA GAGCTGGTGG TTACATCGTC CAGCGTTTCT TGACTGGTAT TCGCCCCCAG 
GAGTACTACT TCCATCACAT GGCCGGCCGT GAGGGTCTTA TCGACACAGC CGTCAAGACA 
TCGCGTTCCG GTTACCTTCA GAGATGTTTG ATCAAGGGCA TGGAAGGTCT CAAGGTCGCT 
TACGACACCT CGGTCAGAGA CTCGGATGGC TCCATCATTC AGTTCCTCTT TGGTGAGGAT 
GGTATCGATA TCTCCAAGCA AAAATACTTG AACGACTTCG AGTTCGTCCT CCGCAACCTG 
AAATCCGAGT TGGCGCATCT CCACTACTCG GAAGAGGGTA CTCAGGCCCT CTTCGAGCAC 
AAGGACGAGA TGATCAAGCG GATGAAGGCT GCCATCAAGT CGAGGGATAC CCGCAACCCG 
CTCAACCCGA TCGAGTCGGA TGTTGACCCT GCCTTGTATG CTTTCGCTAC TTCGGAGAAC 
TTCTTCGAGA AGATGACCAA GTACGTCAAG GAGAATAAGG ATGGCTTAAT CAAGGAGAAG 
AAGGGCGACT CCTCTGGCAT CATTGTTCGC AAGAATGCCG AGAAGATTTT GGCGGCCAAG 
TACATCAGGT CATTGGTGGA GCCTGGTGAG GCTGTTGGTA TCGTGGCCGG TCAGTCCGTC 
GGTGAGCCCT CGACACAGAT GACACTGAAC ACTTTCCATT TGGCTGGTCA CTCTGCCAAG 
AACGTCACAC TGGGTATCCC CCGTCTGCGT GAAATTCTCA TGACAGCTTC TTCCAAGATC 
TCGACTCCTG CCATGACCCT CTACCCCAAC GCTGAGCTCT CTGATACAGA CTGCGAGCGG 
TTTGCCAAGT CCATCAGCAT TCTTCCCCTG GCCGATGTCA CCAAGGAAGT TACCGTCAGG 
GAGAAGATGG GCCGTGGCTT GGGCTACCCT CTTGCCAAGC TCTTCGAGAT CAGGTTGCGC 
TTCTTCCCAT CTGAGGAGTA CTGCAAGACA TACGCGATTG AGATTGCGGA TGTTCTTGCG 
ACTGTTGAGA GGAAGTTCGT GCCCCTGCTT GGTCAGCTCA CCAAGAAGGA AATCAAGAAG 
AAGTACAAGG AGAACTCTGC TGCCACTCCC GAGATCGGTG TCAAGGTTGG CACTGTGGAG 
ATGGCGGCGC CCGACGGCGA GCGTGCTGTC GGCGAGGACG GCGACGCGGA CGACGATGAC 
GACGGTGATG ATGACGCCAC CAACGCCAAG AGCAGAGCCA ATAGACACGA GGCAGTGTCC 
TACGGCCCCA ACGACGACGA TGATGATGCC ATCCAGAAGT CTCTACAGCG CGCGGATGAC 
GGTGAGGAGG AGGGCGAGGA GAGACTTGAG TTCGACGATG AAGGCTACGG CGGTAGCCCT 
GAGCCCGAGG ACCGCAACGC CGATGGCGAT ATGTCCGTGT ACAAGTCTCG ATCACGCGAG 
ACCCGTATCA AGGATAAGAA CGACTTCGTT GCCCGCTTCC ACTGCGATGA GCAAGGCGGC 
GAGTGGTGCG AGATGATCCT CGAGTTTGAT CCCGCGACAC CCAAGATTCT CATGCTCAAC 
CTCGTCACCG AGGCTGTCAA GAAGGCGCTC ATCCAGCAAA TTCCCAGACT CGGCACCGCG 
ACATTCGTTG TGGAGAACAA GGAGCGTCTT GTCCACACCG AAGGCTCCAA CATCAAGGCC 
ATGCAGCAGT ATGCCGACTA CATCAACCCC AACAAGATCA TGACCAACGA TATTGCCCAA 
GTGCTCGAGA TCTATGGTGT TGAGGCCGCC AGGAACAGCA TCGTTCGCGA ATTGAGCGGT 
GTCTTCAAGG GTCACGGTAT CGGTGTCGAC CCCCGCCATC TGAACCTCAT TGGCGACTAC 
ATGACCAGAA ACGGTGGCTT CACTCCTTTC AACCGTATGG GTTTGACCGG CAACGTCAGC 
CCCTTCACCA AGATGAGTTA CGAAACTACC GTCGGCTTCC TCAAGGACGC CGTTCTCGAC 
GGCGATTGGG ACAGCCTTGA CACCCCATCA GGCAGACTGG TTGTCGGTAA GCTGGGTGTT 
ACCGGTACCG GCTCGTTCGA CGTTTTCACC AAGGTGCCTA CTGAGCACCA CTCTCATGTG 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_956006.2
CDS	249..5354
Translation	MNISQPVSSSLDSVEFTFLTDTEIRAVSVRKIENESTFDSLLNPVPGGLYDPALGSWGDSLCTTCNLGQSQCPGHPGHIELPVPVYHPVFMDQAYRLLRAQCVYCHRFRLPRREIHRYSCMLRLLQCGLLKEAQMIDSFGESEFGSAIRQFQLADVPEMEDDEAEEEGNNLNDSTIRQREAYVRAVLRDYRTKVTMRDINKGKHEGAADMRRALVKEFLAAIIKDKKCRTCDGISPVYRKDRYTKIFERDLTQKEKAAMAQAGRKRADALAMKGGKKKNHDDDEGIADIESTASESDNSEGEGEELDENGDVVMADAGSKTKAKEKAEKRALPQRYISSLEVKERLNFLFQKEQEIVSLLFSSKPRPKHAKPMTADMFFIQTLLVPPNKFRPEARMGDQVTEAQQNSLYKLILRGSSMVAQISREVSETAKGNAPEDGRRARDINALYQAWTQLQDAVNSLIDRDKNPVQGAAGKRNEDGIKQKLEKKEGLFRKNMMGKRVNFAARSVISPDPNIETSEIGVPPVFARKLTYPEPVTSHNYHDLKNAVQNGMEKWPGASAIEMENGQIINLRNKSSEERQALANQLLAPTNNNFSGVRNKKVHRHLTNGDVVLMNRQPTLHKPSIMGHRVRVLPGEKTIRMHYANCNTYNADFDGDEMNMHFPQNEIARAEALQLADTDHQYISGTAGKPLRGLIQDHLSVSVILCNKDTFFDRANYHQLIYAALRPESGHIMGDKIELLPPAIIKPVPRWTGKQVITTILKNIKPPNCGDLWMNGSTQVKAHQWGDHAPEEGQVTFRDGEFITGILDKSQLGPSSGGLIHSIHEVYGPSAAGKLLSCMGRLLTRYLAMVAFSCGMDDLVMTPKGEADRKEKIKAAAHIGLEVAAKYVSLEEQKPTPEDPLLLQRLEEVMRNDKKQENLDLLMNQRCAQLSSEITKTCLPAGLQKKFPKNQMQSMTTSGAKGSPVNANLISCNLGQQVLEGRRVPVMVSGKTLPSFKPFDTNARAGGYIVQRFLTGIRPQEYYFHHMAGREGLIDTAVKTSRSGYLQRCLIKGMEGLKVAYDTSVRDSDGSIIQFLFGEDGIDISKQKYLNDFEFVLRNLKSELAHLHYSEEGTQALFEHKDEMIKRMKAAIKSRDTRNPLNPIESDVDPALYAFATSENFFEKMTKYVKENKDGLIKEKKGDSSGIIVRKNAEKILAAKYIRSLVEPGEAVGIVAGQSVGEPSTQMTLNTFHLAGHSAKNVTLGIPRLREILMTASSKISTPAMTLYPNAELSDTDCERFAKSISILPLADVTKEVTVREKMGRGLGYPLAKLFEIRLRFFPSEEYCKTYAIEIADVLATVERKFVPLLGQLTKKEIKKKYKENSAATPEIGVKVGTVEMAAPDGERAVGEDGDADDDDDGDDDATNAKSRANRHEAVSYGPNDDDDDAIQKSLQRADDGEEEGEERLEFDDEGYGGSPEPEDRNADGDMSVYKSRSRETRIKDKNDFVARFHCDEQGGEWCEMILEFDPATPKILMLNLVTEAVKKALIQQIPRLGTATFVVENKERLVHTEGSNIKAMQQYADYINPNKIMTNDIAQVLEIYGVEAARNSIVRELSGVFKGHGIGVDPRHLNLIGDYMTRNGGFTPFNRMGLTGNVSPFTKMSYETTVGFLKDAVLDGDWDSLDTPSGRLVVGKLGVTGTGSFDVFTKVPTEHHSHVL

Target ORF information:

RefSeq Version	XM_950913.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A DNA-directed RNA polymerase I subunit RPA1 (NCU01638), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_950913.3

ORF Insert Sequence:

ATGAATATTT CGCAGCCCGT GTCGTCCTCT CTGGACAGCG TCGAGTTCAC CTTCCTGACC 
GACACCGAGA TCCGCGCCGT CTCGGTCCGC AAGATCGAGA ATGAGAGCAC CTTCGACAGC 
CTGCTGAACC CCGTCCCCGG CGGTCTCTAC GACCCTGCCT TGGGTTCCTG GGGCGACTCA 
CTCTGCACAA CATGTAACCT TGGCCAGTCG CAATGTCCCG GTCATCCCGG CCACATCGAG 
CTCCCCGTGC CCGTCTATCA CCCCGTCTTC ATGGACCAGG CATACCGTCT GCTGCGCGCG 
CAATGTGTTT ACTGCCACCG GTTCCGCCTT CCCAGGAGGG AAATCCACCG CTACTCCTGC 
ATGCTCCGTC TGCTGCAGTG TGGCCTTCTC AAGGAGGCCC AGATGATCGA CTCTTTCGGC 
GAAAGCGAGT TCGGCTCCGC CATCCGCCAA TTCCAGCTGG CCGACGTGCC CGAGATGGAG 
GATGACGAGG CTGAGGAGGA GGGAAACAAC TTGAACGACA GCACCATCCG CCAACGGGAA 
GCATATGTCC GCGCTGTCCT TCGCGATTAT AGGACTAAGG TGACTATGCG CGACATCAAC 
AAGGGCAAGC ACGAAGGCGC CGCCGATATG CGCAGGGCTC TTGTCAAGGA GTTCCTGGCT 
GCCATCATCA AGGACAAGAA GTGCAGGACT TGCGACGGCA TCTCTCCCGT CTACCGCAAG 
GATCGGTACA CCAAGATTTT CGAGCGCGAC CTCACACAAA AGGAGAAGGC CGCCATGGCC 
CAGGCTGGAA GGAAGCGTGC TGACGCGCTG GCCATGAAGG GCGGCAAGAA GAAGAATCAT 
GATGATGATG AGGGGATCGC CGATATTGAG TCGACAGCCA GCGAATCCGA CAACAGTGAA 
GGAGAGGGAG AAGAGCTCGA CGAGAACGGC GATGTTGTCA TGGCGGACGC GGGCAGCAAG 
ACCAAGGCGA AGGAGAAGGC CGAGAAGAGG GCCCTTCCCC AGAGATACAT CAGCTCTCTC 
GAGGTCAAGG AGAGGTTGAA CTTCCTCTTC CAGAAGGAGC AAGAGATTGT CTCTTTGCTC 
TTTAGCTCCA AGCCTCGTCC CAAGCACGCG AAGCCGATGA CCGCCGACAT GTTCTTCATC 
CAGACACTTC TCGTCCCTCC CAACAAATTC AGACCCGAGG CTAGAATGGG CGACCAAGTA 
ACGGAAGCGC AGCAAAACAG TCTCTACAAG CTAATCCTCA GGGGGTCGTC TATGGTCGCC 
CAGATCTCTC GCGAGGTTTC CGAAACCGCC AAGGGTAATG CCCCCGAGGA CGGACGCAGG 
GCCAGGGATA TCAATGCGCT CTACCAGGCG TGGACACAGC TTCAAGACGC CGTCAACTCC 
TTGATCGATC GCGATAAGAA CCCCGTCCAG GGTGCTGCTG GAAAGCGCAA CGAGGACGGT 
ATCAAGCAGA AGCTCGAGAA GAAGGAGGGT CTTTTCCGCA AGAACATGAT GGGTAAGCGT 
GTCAACTTTG CCGCCAGATC CGTCATCTCT CCCGATCCCA ACATCGAGAC CAGCGAGATC 
GGTGTCCCTC CTGTGTTCGC CAGGAAGCTC ACATACCCCG AGCCCGTAAC GAGCCACAAC 
TACCATGATC TCAAGAATGC AGTGCAGAAC GGTATGGAGA AGTGGCCCGG TGCTTCCGCC 
ATTGAGATGG AGAACGGCCA AATCATCAAC TTGAGGAACA AGTCTTCGGA GGAGCGTCAG 
GCCCTTGCCA ACCAGCTCTT GGCCCCGACC AACAACAACT TCAGCGGAGT TCGTAACAAG 
AAGGTCCACC GCCATCTTAC CAACGGTGAT GTCGTCCTCA TGAACCGTCA GCCCACCCTC 
CATAAGCCCT CCATCATGGG TCACAGAGTC AGAGTGCTGC CGGGCGAGAA GACGATTCGC 
ATGCACTACG CCAACTGCAA TACCTACAAC GCCGATTTCG ATGGTGATGA GATGAACATG 
CATTTCCCCC AAAACGAGAT TGCCCGTGCC GAGGCCCTTC AGCTCGCCGA CACTGATCAC 
CAATACATCT CCGGCACCGC TGGCAAGCCT CTCCGCGGTC TCATTCAGGA TCATTTGTCC 
GTTTCCGTTA TTCTGTGTAA CAAGGACACC TTCTTCGATC GGGCCAACTA CCATCAACTC 
ATCTACGCCG CTCTTCGGCC CGAAAGTGGT CACATTATGG GAGACAAGAT TGAGCTTTTG 
CCGCCGGCGA TCATCAAGCC GGTTCCCCGC TGGACGGGTA AGCAAGTCAT TACCACAATC 
CTCAAGAACA TCAAGCCCCC AAATTGCGGT GATTTGTGGA TGAACGGCTC TACACAAGTC 
AAGGCTCATC AGTGGGGTGA CCATGCCCCA GAAGAAGGCC AGGTCACTTT CCGTGATGGA 
GAGTTCATCA CCGGTATCCT CGACAAGTCT CAGCTGGGTC CCAGCAGTGG TGGTTTGATT 
CACTCGATTC ACGAAGTTTA CGGCCCTTCA GCTGCTGGTA AGCTCCTCAG TTGCATGGGT 
CGTCTCCTCA CTCGTTACCT GGCCATGGTT GCCTTTTCGT GCGGTATGGA CGATCTAGTC 
ATGACGCCCA AGGGAGAGGC AGACAGAAAG GAGAAGATCA AGGCCGCCGC TCACATCGGT 
CTCGAGGTCG CCGCCAAGTA TGTGTCGCTC GAGGAGCAGA AGCCTACTCC CGAAGATCCC 
CTTCTCTTGC AACGCTTGGA GGAAGTTATG CGCAACGACA AGAAGCAAGA GAACTTGGAT 
CTCTTGATGA ACCAACGCTG CGCCCAACTT TCTAGCGAGA TCACCAAGAC ATGTCTTCCT 
GCTGGTCTGC AAAAGAAGTT CCCCAAGAAC CAAATGCAAA GTATGACAAC ATCAGGTGCT 
AAGGGTTCTC CTGTCAACGC CAACCTCATC TCTTGTAACT TGGGTCAGCA GGTTTTGGAA 
GGTAGACGTG TCCCCGTCAT GGTCAGCGGC AAGACTCTCC CTAGTTTCAA GCCCTTCGAT 
ACCAACGCCA GAGCTGGTGG TTACATCGTC CAGCGTTTCT TGACTGGTAT TCGCCCCCAG 
GAGTACTACT TCCATCACAT GGCCGGCCGT GAGGGTCTTA TCGACACAGC CGTCAAGACA 
TCGCGTTCCG GTTACCTTCA GAGATGTTTG ATCAAGGGCA TGGAAGGTCT CAAGGTCGCT 
TACGACACCT CGGTCAGAGA CTCGGATGGC TCCATCATTC AGTTCCTCTT TGGTGAGGAT 
GGTATCGATA TCTCCAAGCA AAAATACTTG AACGACTTCG AGTTCGTCCT CCGCAACCTG 
AAATCCGAGT TGGCGCATCT CCACTACTCG GAAGAGGGTA CTCAGGCCCT CTTCGAGCAC 
AAGGACGAGA TGATCAAGCG GATGAAGGCT GCCATCAAGT CGAGGGATAC CCGCAACCCG 
CTCAACCCGA TCGAGTCGGA TGTTGACCCT GCCTTGTATG CTTTCGCTAC TTCGGAGAAC 
TTCTTCGAGA AGATGACCAA GTACGTCAAG GAGAATAAGG ATGGCTTAAT CAAGGAGAAG 
AAGGGCGACT CCTCTGGCAT CATTGTTCGC AAGAATGCCG AGAAGATTTT GGCGGCCAAG 
TACATCAGGT CATTGGTGGA GCCTGGTGAG GCTGTTGGTA TCGTGGCCGG TCAGTCCGTC 
GGTGAGCCCT CGACACAGAT GACACTGAAC ACTTTCCATT TGGCTGGTCA CTCTGCCAAG 
AACGTCACAC TGGGTATCCC CCGTCTGCGT GAAATTCTCA TGACAGCTTC TTCCAAGATC 
TCGACTCCTG CCATGACCCT CTACCCCAAC GCTGAGCTCT CTGATACAGA CTGCGAGCGG 
TTTGCCAAGT CCATCAGCAT TCTTCCCCTG GCCGATGTCA CCAAGGAAGT TACCGTCAGG 
GAGAAGATGG GCCGTGGCTT GGGCTACCCT CTTGCCAAGC TCTTCGAGAT CAGGTTGCGC 
TTCTTCCCAT CTGAGGAGTA CTGCAAGACA TACGCGATTG AGATTGCGGA TGTTCTTGCG 
ACTGTTGAGA GGAAGTTCGT GCCCCTGCTT GGTCAGCTCA CCAAGAAGGA AATCAAGAAG 
AAGTACAAGG AGAACTCTGC TGCCACTCCC GAGATCGGTG TCAAGGTTGG CACTGTGGAG 
ATGGCGGCGC CCGACGGCGA GCGTGCTGTC GGCGAGGACG GCGACGCGGA CGACGATGAC 
GACGGTGATG ATGACGCCAC CAACGCCAAG AGCAGAGCCA ATAGACACGA GGCAGTGTCC 
TACGGCCCCA ACGACGACGA TGATGATGCC ATCCAGAAGT CTCTACAGCG CGCGGATGAC 
GGTGAGGAGG AGGGCGAGGA GAGACTTGAG TTCGACGATG AAGGCTACGG CGGTAGCCCT 
GAGCCCGAGG ACCGCAACGC CGATGGCGAT ATGTCCGTGT ACAAGTCTCG ATCACGCGAG 
ACCCGTATCA AGGATAAGAA CGACTTCGTT GCCCGCTTCC ACTGCGATGA GCAAGGCGGC 
GAGTGGTGCG AGATGATCCT CGAGTTTGAT CCCGCGACAC CCAAGATTCT CATGCTCAAC 
CTCGTCACCG AGGCTGTCAA GAAGGCGCTC ATCCAGCAAA TTCCCAGACT CGGCACCGCG 
ACATTCGTTG TGGAGAACAA GGAGCGTCTT GTCCACACCG AAGGCTCCAA CATCAAGGCC 
ATGCAGCAGT ATGCCGACTA CATCAACCCC AACAAGATCA TGACCAACGA TATTGCCCAA 
GTGCTCGAGA TCTATGGTGT TGAGGCCGCC AGGAACAGCA TCGTTCGCGA ATTGAGCGGT 
GTCTTCAAGG GTCACGGTAT CGGTGTCGAC CCCCGCCATC TGAACCTCAT TGGCGACTAC 
ATGACCAGAA ACGGTGGCTT CACTCCTTTC AACCGTATGG GTTTGACCGG CAACGTCAGC 
CCCTTCACCA AGATGAGTTA CGAAACTACC GTCGGCTTCC TCAAGGACGC CGTTCTCGAC 
GGCGATTGGG ACAGCCTTGA CACCCCATCA GGCAGACTGG TTGTCGGTAA GCTGGGTGTT 
ACCGGTACCG GCTCGTTCGA CGTTTTCACC AAGGTGCCTA CTGAGCACCA CTCTCATGTG 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU01638 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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