NCU11050 cDNA ORF clone, Neurospora crassa OR74A

The following NCU11050 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU11050 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf09607 XM_001728493.2
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Neurospora crassa OR74A DUF455 domain-containing protein (NCU11050), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf09607
    Clone ID Related Accession (Same CDS sequence) XM_001728493.2
    Accession Version XM_001728493.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4107bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product DUF455 domain-containing protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). On Feb 23, 2015 this sequence version replaced XM_001728493.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGTCCA CGGTTCCCTC CCCAGAACCC GCTTCTCCTC CTCCTCCTGC TGCTGCTGCT 
    GCTGCTGCTG CTGCTGCTCC AAAACCATCC TCATTAAAAT CACCCTGGAC ACCACCCCCC
    CTCTCATCCC ACAACACAGC CCTCGCCATA ATTCCCCCTC CCCATCTCTG GGCCCCGATC
    AATGCCCTTC GCTCCCTCTA CGACAAAGCA TACACTAAAT GGCCACCTCA CATCAACCTC
    GTCTACCCCT TTGTCGAGCC AGAAGATCTG CAAGATACAA TGGAGTTGGC TCCGAGGGGG
    GTTCTTGAGG GGAGGAGGAA AAGAAATGAG GCAAGGGGTA TAAAGCTGGA TGAGGGGGCG
    CAGGCATGGA CGGTGGGGGG GCCTGATGGG AAGCAGCAGC AGTTCGGACG ATGCCACCCA
    GGAGGAGGAG GAGGAGGAGG TGGGGGGCCG GTTGTGTTTG AGGAGGAGGA TAAAGTGAGG
    GTGAGGTTGG ATGGGGTGGG GGTTTTTGAG CACAGGAAGA AGCATAATAC GATATATCGG
    TGTGACGAAA CTAACCTCAC AAGCACGTCG TCGTCGTTGT CAAGACTGAG AGCGGAAATA
    CTTGGGGAAT TGGGATACCA CCAGCAGGTT GAAGGCGAAT ACAAACCGCA CATGACAATT
    GCCCAGTCTG AAGACAGCCG ATCCTCAGCA CACAAGTTCC TGGTGGAAAA AGTGGGATTG
    CTGCCGACGG TGGAGTGGGA GGTTGAGCAG GTTTGGGTGT TGGTTAGGGA TCAGAGCGAT
    GGTCCTAGTA AGGGGAAGAT GAAGGTTTGG GGCACGATAA ACTTGGGGGA GGAGGTGGGA
    TGTTCGATAC ATGAGAATCC GGTTGATTGG GAGGAGGCTT TGAAAATGGA TGCGGGGTGG
    GAGGGGGAGG AGAGGGATGT GGAGGAGATG CAGAGGAATT GGAGGTTTGA TGAGGAGAGG
    GGGGTTTGGG TGAGGGATGA TGCTGGGAAG GGAGAGGAAG AGACAAAGAC GCCAGAATTG
    CTAAGCATAG CATCCTGGAA CGTCCTTGCT GATTTCGAGC ACTCCTCTTC TTCATCCAAC
    GATCAAGAAG CTCGATACCG CCTCATCCTC CGCAACCTCC TATCTCCTAG CGCCAAAGCA
    GATATTCTTC TCCTTCAAGA AGTCACAGAC TCTTTCCTTT GTTTCATTCT CTCCCCCTCC
    AACACATCTC CCGAATCCCA GGCCTTACGA CACCTCTACC CTTACGTCTC CCACCCCCCT
    CCCTCTCAAC CCGATGCATC TCCCCTCCCC AACTTTCTCA ACCAAGTTAT CTTGTCCAAA
    CACCCCTTCT CCTGGTCTTG GCTCCCTTTT GCTAGGAAGC ACAAAGGCTC TGTCATTGCC
    CGGTTTCCTA CCATCCACGG AAGCAATCCT GGTCAGCCCC TCGTGCTGGC AGCGGTTCAT
    TTGAGTCATG GACTGACGGA CATGACTGTA GCTGCCAAGA AGGGAGAGAT GCAGCGGGTA
    ATTGGGCATC TGAAGGAGAA ATTTCCGGAG AATCCGTGGG TGGTTGCCGG GGACTTCAAT
    CTGACATCTT CAAGCTGGAC TGTTGAGCAA GCTGTGAAAG CTGGGAAGAT TTCCCGGGAA
    ACCATGGCGA CACTGAATAG TGTCGAAAGG ATGTGGAAGG AGGAAGAGGG CCTAGGGGTG
    GAAGACGCTT GGGTGGTAGG TCGTGCTGCT TTGGGAGAGG GATGGACTCA AGAAGAGGAG
    GACATCATGG ACGACAGGGA CGAGGAAGGA GAGGATAATA TGTTGTTTTC CGGCGAGCAA
    GGAGCTACCT ACGATCCGAC CAGGAATGAT GTCGCAGCCA AGGTGGTGGG TAGTGGAGGG
    AATATGCGGC CGCAGAGGTA TGATCGGATT TTGGTCAGGG GTCAACAGGT GTTGAGGATC
    GGGAGGTTCA ATCGGTTTGG ATTCCTCAAG AGTGATCAGG AGAAAGGACA GGGTCATGAG
    ATGGGGCAGT ATGGGAGTGA TCATTGGGGG GTGAGGTGTC TTTTAAAGAT TGTTCAGCAG
    GATACCACTG AGCAGACTGA GAAGGCGGCT GAGGAAAAGA GCGGTGCCAC TACGTTAGAA
    ACGTCTATCA AGCTGGTTAC ACCACTCGAC GGCCCTCTCT CCGAGCCAGG ATCGCTGAAA
    GAGGCCATGG CTGAGCTCAA CATCCTTCCT TCCCAGGAAG AGACAGACAA GCGCAAAGCA
    GCTTTCCAAG CCCTCAAGGC CATCCTCCTT GACACACCTC CCCCGAGTCA GTCTCCCCAG
    CCACCAACCA CCGATCTTAC TACCACCTCC GAGACCCAAC CCCGATCCAA CCAGCCGCCC
    TTGATCATAA CCCCTGTCGG TTCCTTCGCT CTCGGAGTTC ACACCTCCTC CTCTGACATC
    GACGTCCTAG CCATCGGTCC CTTCTCCACC ACCACCTTCT TCTCCCTCGC CATCCAACGT
    CTCCGGCGCG CCTCCTCTTC CCCGCTCTAC TCTCAGGAAC ACCAACCCAA AATCCTCCGC
    CGCATCTCTT CCTCAACCGG CACCATGCTC GAACTCTCCC TCTGCCAAAT CAAATTCGAC
    CTGCAATACT GTCCCGCGGC ACACATTGCC TCGCACTGGC CTCGCATCTT ATCAACAGCC
    CCGCCTGGAA ACGCCGTCTG GTCTTTGCCA GCGCTGACGC TGACGAAACT GAAAGCGGCC
    AGGGATCTGG ATTATTTGCG GCGGACGGTG CCTGACTTGG CAGTGTTTAG GCAAGCCCAT
    AGAGCTGTGA GGAGCTGGGC GAAAGGTAGG GGGGTGTATG CCCAGCGGTT TGGGTATTTG
    GGAGGGATAC AGATCTCGGT CATGTTGGCA AGGGTATATA AAGCTCTTGC GCTTGGGGCT
    GGGGGAGTTA GTGGTGTTGG GGTGGAGGAG TTGGTGGCGA GTTTCTTTGA AGAGTATGCT
    CAGTTTGATT GGGAGAAGGA TATGGTGTAT GATGAGGTTT GTCCTTGGTT GAGGCTGAAG
    CGGGAAAAAG GAGGGGAGAA GGAGGTGTAT AGACGAACGG GGAGGGAGGC GATGGTTGTG
    CTTGGGTGGT TTGGACCGGG ACTGAACACT AGCGGGCAGG CGAGTGTTTG GAGTGTTAGG
    ACGCTGAAGG ACGAGTTTAA GCGGGCGAGG GACTTGTTGG TGAAGGGAGA AGTGAAGAGC
    TGGAAGGAGT TTTTAAAGGG AAGTGGTGCA GAGGAGTTCT TGACGGGGTA CAAGAGCTAT
    GTCAAGGTTG ATGTGCAGTA TTGGGGATTG TCGTCGTCGA AGGGTGCGCA GTTTGTGGGC
    TGGTTGGAGA GTCGGTGCGT GATGCTCCTT GTTGATCTTC ATAGGAGGGC ACCCGGGGTG
    TATGTCAGGA TGTGGCCTGG ACGCTTTGTC GAGGCCGACA CTGCCTCTCA ACAAGCCGAA
    AATGATGAGC AGACGGCCAC CGAAGGAACC GGACCCCGCG ACTACCAAGC CTCCTACCTC
    CTGGGCCTCG ACGCCGATAC CACTACCAGC AAAGAAGACC TCAAACTCGC GCTTGGCGCC
    ATCCAAACCG CGCTTCAACG CTTTGAGGAA GCCATCCGGT CAGACGAGAA GTATTTTGAT
    GCCTCGAGCA GCTGGATGAG CGCCAGCATA GTGAAACGCT CCGAACTGCG CAACCTCCAA
    CTCGACACTC GCAACGACTG GGTAGGCGAG TACACTCCTG GCGAAGACGA GTCAGACTCT
    GACCCTGAGG ATGACGACGA GGAAGACGCT TCATTGCTTG AGGACCTCGA CGACACCGCT
    CCGAAGAAGA AAAAGGGCAA AAACAAGAAA CCGATAGAGG AGACGCAGCA ACCAAGACTG
    GAACCAGGCC CGAAGCTTCG CTCGGCAGGG GACGTGATGA ACAGACTACG CTGGGACTCG
    GCCATCGACA GTTCTGACTA CGTGGTTGGT TACGAGGATC GGTTCCTAGG AGTGAAGGAG
    AGAGCACTGG ATATGTGGAA GACGGAGCAG ACGGATGAGG AATTCATCCC GCAGCATCGC
    ATTGTGTATT TCAAGAGGAG GAGTGATGGA CAGGTGGTGT GGGATCGGAA GACGAAGAAG
    GATGAGGTTT TTGGGAGTGG GTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001728545.1
    CDS545..4651
    Translation

    Target ORF information:

    RefSeq Version XM_001728493.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A DUF455 domain-containing protein (NCU11050), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001728493.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGTCCA CGGTTCCCTC CCCAGAACCC GCTTCTCCTC CTCCTCCTGC TGCTGCTGCT 
    GCTGCTGCTG CTGCTGCTCC AAAACCATCC TCATTAAAAT CACCCTGGAC ACCACCCCCC
    CTCTCATCCC ACAACACAGC CCTCGCCATA ATTCCCCCTC CCCATCTCTG GGCCCCGATC
    AATGCCCTTC GCTCCCTCTA CGACAAAGCA TACACTAAAT GGCCACCTCA CATCAACCTC
    GTCTACCCCT TTGTCGAGCC AGAAGATCTG CAAGATACAA TGGAGTTGGC TCCGAGGGGG
    GTTCTTGAGG GGAGGAGGAA AAGAAATGAG GCAAGGGGTA TAAAGCTGGA TGAGGGGGCG
    CAGGCATGGA CGGTGGGGGG GCCTGATGGG AAGCAGCAGC AGTTCGGACG ATGCCACCCA
    GGAGGAGGAG GAGGAGGAGG TGGGGGGCCG GTTGTGTTTG AGGAGGAGGA TAAAGTGAGG
    GTGAGGTTGG ATGGGGTGGG GGTTTTTGAG CACAGGAAGA AGCATAATAC GATATATCGG
    TGTGACGAAA CTAACCTCAC AAGCACGTCG TCGTCGTTGT CAAGACTGAG AGCGGAAATA
    CTTGGGGAAT TGGGATACCA CCAGCAGGTT GAAGGCGAAT ACAAACCGCA CATGACAATT
    GCCCAGTCTG AAGACAGCCG ATCCTCAGCA CACAAGTTCC TGGTGGAAAA AGTGGGATTG
    CTGCCGACGG TGGAGTGGGA GGTTGAGCAG GTTTGGGTGT TGGTTAGGGA TCAGAGCGAT
    GGTCCTAGTA AGGGGAAGAT GAAGGTTTGG GGCACGATAA ACTTGGGGGA GGAGGTGGGA
    TGTTCGATAC ATGAGAATCC GGTTGATTGG GAGGAGGCTT TGAAAATGGA TGCGGGGTGG
    GAGGGGGAGG AGAGGGATGT GGAGGAGATG CAGAGGAATT GGAGGTTTGA TGAGGAGAGG
    GGGGTTTGGG TGAGGGATGA TGCTGGGAAG GGAGAGGAAG AGACAAAGAC GCCAGAATTG
    CTAAGCATAG CATCCTGGAA CGTCCTTGCT GATTTCGAGC ACTCCTCTTC TTCATCCAAC
    GATCAAGAAG CTCGATACCG CCTCATCCTC CGCAACCTCC TATCTCCTAG CGCCAAAGCA
    GATATTCTTC TCCTTCAAGA AGTCACAGAC TCTTTCCTTT GTTTCATTCT CTCCCCCTCC
    AACACATCTC CCGAATCCCA GGCCTTACGA CACCTCTACC CTTACGTCTC CCACCCCCCT
    CCCTCTCAAC CCGATGCATC TCCCCTCCCC AACTTTCTCA ACCAAGTTAT CTTGTCCAAA
    CACCCCTTCT CCTGGTCTTG GCTCCCTTTT GCTAGGAAGC ACAAAGGCTC TGTCATTGCC
    CGGTTTCCTA CCATCCACGG AAGCAATCCT GGTCAGCCCC TCGTGCTGGC AGCGGTTCAT
    TTGAGTCATG GACTGACGGA CATGACTGTA GCTGCCAAGA AGGGAGAGAT GCAGCGGGTA
    ATTGGGCATC TGAAGGAGAA ATTTCCGGAG AATCCGTGGG TGGTTGCCGG GGACTTCAAT
    CTGACATCTT CAAGCTGGAC TGTTGAGCAA GCTGTGAAAG CTGGGAAGAT TTCCCGGGAA
    ACCATGGCGA CACTGAATAG TGTCGAAAGG ATGTGGAAGG AGGAAGAGGG CCTAGGGGTG
    GAAGACGCTT GGGTGGTAGG TCGTGCTGCT TTGGGAGAGG GATGGACTCA AGAAGAGGAG
    GACATCATGG ACGACAGGGA CGAGGAAGGA GAGGATAATA TGTTGTTTTC CGGCGAGCAA
    GGAGCTACCT ACGATCCGAC CAGGAATGAT GTCGCAGCCA AGGTGGTGGG TAGTGGAGGG
    AATATGCGGC CGCAGAGGTA TGATCGGATT TTGGTCAGGG GTCAACAGGT GTTGAGGATC
    GGGAGGTTCA ATCGGTTTGG ATTCCTCAAG AGTGATCAGG AGAAAGGACA GGGTCATGAG
    ATGGGGCAGT ATGGGAGTGA TCATTGGGGG GTGAGGTGTC TTTTAAAGAT TGTTCAGCAG
    GATACCACTG AGCAGACTGA GAAGGCGGCT GAGGAAAAGA GCGGTGCCAC TACGTTAGAA
    ACGTCTATCA AGCTGGTTAC ACCACTCGAC GGCCCTCTCT CCGAGCCAGG ATCGCTGAAA
    GAGGCCATGG CTGAGCTCAA CATCCTTCCT TCCCAGGAAG AGACAGACAA GCGCAAAGCA
    GCTTTCCAAG CCCTCAAGGC CATCCTCCTT GACACACCTC CCCCGAGTCA GTCTCCCCAG
    CCACCAACCA CCGATCTTAC TACCACCTCC GAGACCCAAC CCCGATCCAA CCAGCCGCCC
    TTGATCATAA CCCCTGTCGG TTCCTTCGCT CTCGGAGTTC ACACCTCCTC CTCTGACATC
    GACGTCCTAG CCATCGGTCC CTTCTCCACC ACCACCTTCT TCTCCCTCGC CATCCAACGT
    CTCCGGCGCG CCTCCTCTTC CCCGCTCTAC TCTCAGGAAC ACCAACCCAA AATCCTCCGC
    CGCATCTCTT CCTCAACCGG CACCATGCTC GAACTCTCCC TCTGCCAAAT CAAATTCGAC
    CTGCAATACT GTCCCGCGGC ACACATTGCC TCGCACTGGC CTCGCATCTT ATCAACAGCC
    CCGCCTGGAA ACGCCGTCTG GTCTTTGCCA GCGCTGACGC TGACGAAACT GAAAGCGGCC
    AGGGATCTGG ATTATTTGCG GCGGACGGTG CCTGACTTGG CAGTGTTTAG GCAAGCCCAT
    AGAGCTGTGA GGAGCTGGGC GAAAGGTAGG GGGGTGTATG CCCAGCGGTT TGGGTATTTG
    GGAGGGATAC AGATCTCGGT CATGTTGGCA AGGGTATATA AAGCTCTTGC GCTTGGGGCT
    GGGGGAGTTA GTGGTGTTGG GGTGGAGGAG TTGGTGGCGA GTTTCTTTGA AGAGTATGCT
    CAGTTTGATT GGGAGAAGGA TATGGTGTAT GATGAGGTTT GTCCTTGGTT GAGGCTGAAG
    CGGGAAAAAG GAGGGGAGAA GGAGGTGTAT AGACGAACGG GGAGGGAGGC GATGGTTGTG
    CTTGGGTGGT TTGGACCGGG ACTGAACACT AGCGGGCAGG CGAGTGTTTG GAGTGTTAGG
    ACGCTGAAGG ACGAGTTTAA GCGGGCGAGG GACTTGTTGG TGAAGGGAGA AGTGAAGAGC
    TGGAAGGAGT TTTTAAAGGG AAGTGGTGCA GAGGAGTTCT TGACGGGGTA CAAGAGCTAT
    GTCAAGGTTG ATGTGCAGTA TTGGGGATTG TCGTCGTCGA AGGGTGCGCA GTTTGTGGGC
    TGGTTGGAGA GTCGGTGCGT GATGCTCCTT GTTGATCTTC ATAGGAGGGC ACCCGGGGTG
    TATGTCAGGA TGTGGCCTGG ACGCTTTGTC GAGGCCGACA CTGCCTCTCA ACAAGCCGAA
    AATGATGAGC AGACGGCCAC CGAAGGAACC GGACCCCGCG ACTACCAAGC CTCCTACCTC
    CTGGGCCTCG ACGCCGATAC CACTACCAGC AAAGAAGACC TCAAACTCGC GCTTGGCGCC
    ATCCAAACCG CGCTTCAACG CTTTGAGGAA GCCATCCGGT CAGACGAGAA GTATTTTGAT
    GCCTCGAGCA GCTGGATGAG CGCCAGCATA GTGAAACGCT CCGAACTGCG CAACCTCCAA
    CTCGACACTC GCAACGACTG GGTAGGCGAG TACACTCCTG GCGAAGACGA GTCAGACTCT
    GACCCTGAGG ATGACGACGA GGAAGACGCT TCATTGCTTG AGGACCTCGA CGACACCGCT
    CCGAAGAAGA AAAAGGGCAA AAACAAGAAA CCGATAGAGG AGACGCAGCA ACCAAGACTG
    GAACCAGGCC CGAAGCTTCG CTCGGCAGGG GACGTGATGA ACAGACTACG CTGGGACTCG
    GCCATCGACA GTTCTGACTA CGTGGTTGGT TACGAGGATC GGTTCCTAGG AGTGAAGGAG
    AGAGCACTGG ATATGTGGAA GACGGAGCAG ACGGATGAGG AATTCATCCC GCAGCATCGC
    ATTGTGTATT TCAAGAGGAG GAGTGATGGA CAGGTGGTGT GGGATCGGAA GACGAAGAAG
    GATGAGGTTT TTGGGAGTGG GTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B