pex6 cDNA ORF clone, Neurospora crassa OR74A

The following pex6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pex6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf08561 XM_960183.2
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Neurospora crassa OR74A peroxisomal biogenesis factor 6 (pex6), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf08561
    Clone ID Related Accession (Same CDS sequence) XM_960183.2
    Accession Version XM_960183.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product peroxisomal biogenesis factor 6
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_960183.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGACCGCCC CCAACTCCAC CCCTGCCTCG AGCAGGAAAA GGGTCCGAAG ACGCAGACAG 
    GACAAGCCCG CCTTGTCAGC CAAATTGGTC CTCGATGCCC ATGTCAAGGG CGATGTTGGT
    GTCATTGCTA CCGACCTCTT CGCCGACCTC TTCCCTCATC TCTGGGCTAA TGGCGACAGC
    CAGGACACCG TTCATGTTGC AATCGCCCCT TGGGCTCCAA GCCAAACCCC CGAGGCTAAC
    AATTGGTCCA TTGTACCCGT CATTCGATCT TCCACCGTTG CGCCGTCCAC TGTACAGTTC
    TCGCCCTCAT CGCTGGCCCT CCAGTCTTTC GCGACACACC TCCAGCAGGC TGCGCCCTCA
    AAGCTCTCCG GCCACAACAA AGGTGGCATT GAGATCCTGA TCTTGGATGT TTCGCCCATC
    GAGCTCGATA CCGTCTTTGT GAACCTTGAC GGAGAATTGG CTAAGAGACT GGAAGATGGG
    GAGGGAACCT TCTTCCGCGA ACACCCAGTC AACGGAAAGG GCAAGGCTCG TGCCGATGCT
    ACCCCTGAGG AACACTTGAC AGCTGCTTTG CGCACTGCCC TCGGTACCCT AAAGATTGTG
    CATTCTGGCG ATTTCTTCCC ATTGCCACTA CCACCTCACC CAGTTACCCA CCAACCTCCA
    AACCCAGGAC GGATAACGCT TTGCGAACCT GTGGCTCAGG GTATCCTCGC CCCTACTACA
    AAGATCATCG TGTCGAGAGT GCGCACCAGC AAGACCAAAC GTAGCTCCGC ATCTGGCCAG
    CAACAGGGGC GTAAACTGAA CGGAGTGGCC GAAGACGACG AAGACGCCTA CTTCTCGGCT
    GCCGAGGAAG ACCGCGACAG CAAGACCAAT GCCCAAACGG CTGAAACAGA TGCAGACGAT
    ACCGAATTCG AGCCACAGGC TGGAGAAGAT GAGGACAACT TGTCGGATGA TTCATTAGAC
    GAAATAATAT CCCTTCAAGT TCCTACTCTA CCCACTACGG TTACCGGCCA ATCTACAATA
    GGAACGGGCA CCCCGACGAT GCTCAGGGGC AGGAAGACCA ACGGTCCTGG GTCGGTTATC
    TCTAGTTATA CTGCCACCAC CGCCAGACCA GACCGACCCC GTGGCCGGCT CTTCAAGGCG
    CATGGATTAA TGAAACCTAT CCCACCTGAT CTGCTCCACC CTAAACCTAC ACCCGAAGAC
    GACGAAGAAG CGTGCATCTA CGTGGATATG AGGGATCTCA CCAGGGTGGG CTGTTTCTCT
    GGAGACTGGA TACGAGTCGA GGCGGCCTCT GAACCGCCCT CCAACGGGCT CGGCGCGTTT
    GGTTTGGGAA GCTTCGTGGA ACAGGAAGCA GACGAGATTA ACTGGAGACC CGCGAGGGTT
    TTCGGCCTGC CTGAAGGGTA TTCGAGCCGT TCAGCGACCA GGTCACACCA CTCTAGACAT
    GATAGCCGGA ACTCGTCCTT CTTTGAGGCA CAGTCCCAAA AGTCGAATCC ACCGGCTTAT
    ACTTCGCCTA TTCTTCTCGC AAACTTAGAC AACCCTCCCT ATCTTCGTCT ATCACCGATC
    AAGCGCCCTT CGAGCTCCAT CATTAAGGGC GTTGCTGCTC AGTCAAAGAC CAGCTCAACC
    TTGAAGCCAC CATTTGCTCG CGAGGTCATC CTACAACATG TGAGAACACC CACAGCGGTC
    GAGAGGGACG TACAGTCTGC GGTCATGGCT GGCCTGAAAC ACTATTTTGA GAGGAGATTG
    CGGGTTCTCA GGACTGGAGA TCTCATTGCT GTACCCATTG ACACCCAGTT GGGCAAGGCT
    CTCCAGGAGA GTACAATCCC TGGTGAGGAT TCTGCCATCG ATGAGGTCTT GGGTCTGATA
    GCTGCAACAC GTCCCGGACA GAGCCTACAT TATGACGATG TTGCCTGGTT CAGGGTTGGT
    CACGTCCAGG CCATGAAGCA AGACACCACA GAAGCAGTCG ATGGCGAGGA GGCAGAGGAT
    CTATGGGGCG GAATCGCCTG TGTAGATATC TCGCTCACAC ATTTAGAACA GTCAGGATCT
    GTCACGGGCC GCATTCCGGG CACGATCTCC AACAGCTGGC AGTATTACCT GGGTATCAGG
    AAGCTTCCTA AGCACCAGCA AAACCCAGGG CTACCTGCTC AACTGCAAAC GCTAGAGCCC
    GAGAAGCAGT ATGTCTCATC ACTCCGAAGG AAACTTCGCG AGCTCATGGC TGCCGCCACC
    AGTAAACCCG CGCTTCATCT CAACCTACCA CCACTGGCCA TTCTTCTTGT GTCGACGCAA
    AGGCATATCG GCAAAGCAGC CACAGCCATG CAAGCCTGTT CAGATATTGG TCTGCACACA
    TTTGCCATAG ACGCTTATGA CATTGTGAAT GATGGCGGTG GTGGAGGTGG TAGTGATGTC
    AAGACAGCTG GGTTCTTGAC ATCGCGCGCT GAGCGTGCTA TGAGCTGTGG TCCCGAATCA
    TGCGTCCTCC TGGTCCGCCA CATTGAAGCC CTTACTGCCG ACCGCATGGT TTCTTCCATC
    AAGGAGATCT TGGCCGACGC TAGGGTTTTG ATCGCCACCA CCACCGAGGT TGAGAAGGTT
    CCCGACGGCA TCCGTGCCCT CTTTACACAC GAGTTGGAGA TGAGTGCCCC CGACGAGCAA
    GAGAGAGAGG GTATCCTCAG CTCCATCCTC GCCGACCGCG GCATCGGTCT TGACCATGGG
    GTAGATCTCA GCGGCATCGC CCTCAAGACT GCCGCGCTCG TAGCAGGCGA TCTCGTCGAC
    GTTGTTGACC GTGCCTTGGT TGCGCAGCGA TCCCGCCTCG AGAAGCTCAC CGCCAAAGCG
    ACCGGCAGCA TCACCTTCCG TGACGTTCAG CTCGCCGGTG GCCCAGCCGC CTCTGGCCTG
    ACCAAGCAGG ATTTTGAGTT AGCCGTCGAT GCCGCCCGCA AGAACTTTGC TGATTCCATC
    GGCGCGCCGA AGATCCCCAA CGTCACATGG GACGACGTCG GTGGCCTAGG CAACGTCAAA
    GACGCCATCA CCGAGACCAT CCAGCTTCCG CTTGAGCGCC CCGAGCTGTT CGCCAAGGGC
    ATGAAGAAGC GCTCCGGTAT CCTGTTTTAC GGACCTCCTG GTACCGGCAA GACGCTCCTG
    GCCAAGGCCA TCGCGACCGA GTACAGCCTC AACTTCTTCA GCGTCAAGGG TCCCGAGCTG
    TTGAACATGT ACATTGGTGA GTCCGAAGCC AACGTGCGGA GGGTGTTCCA GCGTGCCCGC
    GACGCCCGGC CTTGCGTCGT CTTCTTCGAC GAGCTGGACT CCGTCGCCCC CAAAAGAGGA
    AATCAGGGCG ACAGCGGCGG TGTCATGGAT CGTATCGTGT CGCAGCTGTT GGCGGAGCTC
    GACGGCATGT CGGGCGGCGA AGGCGGAGGC GGCGGTGTGT TCGTCATCGG TGCGACCAAT
    AGACCTGATT TGCTGGATCC TGCACTGCTG AGACCCGGTC GCTTCGATAA GATGTTGTAC
    CTGGGTGTCT CGGACACACA CGACAAGCAG GTAACTATCA TGGAGGCTTT GACTAGAAAA
    TTCACTCTCC ACCCAACCGT CTCCCTCCGC TCTGTCGCCG AGCGTCTCCC TTTCACCTAC
    ACCGGCGCCG ACTTCTACGC CCTCTGCTCT GACGCCATGC TCAAGGCCGT TACCCGCCAG
    GCCACCTTGG TCGACACCAA GATCCGCGAG CTCAACGCCG CCGCCGGTCC CGAGGGAAAA
    CAGATCTCGA CTGCCTACTT CTTTGATCAT TATGCCACCA AGGAGGACAT CAGCGTCATG
    GTAACCGAGC AAGACTTTTT GGATGCCCAC AGGGAACTGG TGCCCTCCGT CAGTGCGGGC
    GAGTTGGAGC ACTATGAGCA GGTTAGGGCT ATGTTTGAGG GGGCTAAGGA TAAGGACAAG
    AAGAAGGAGG GTGCTGGTGG TGATGGCAAC GGAGTGGATG GACTTGAGAG CGGTAATAGG
    AATGGGATGC TGGCTATTAC AATGGGTGAT GACGGCGCTG GAGCTGAATC GACGAAGAAG
    GACGGCAAAG GAAAGGGAAA GGCGGCTGAT AACGAGGTCA ATGGTGCTGG TGAGGGGAAG
    GGCAAGGAAG GCGTTTCGCC TTTCCAGGAA TCGGGAGGAG ATGAGGATGA GGGGTTATAT
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_965276.1
    CDS526..4671
    Translation

    Target ORF information:

    RefSeq Version XM_960183.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A peroxisomal biogenesis factor 6 (pex6), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_960183.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGACCGCCC CCAACTCCAC CCCTGCCTCG AGCAGGAAAA GGGTCCGAAG ACGCAGACAG 
    GACAAGCCCG CCTTGTCAGC CAAATTGGTC CTCGATGCCC ATGTCAAGGG CGATGTTGGT
    GTCATTGCTA CCGACCTCTT CGCCGACCTC TTCCCTCATC TCTGGGCTAA TGGCGACAGC
    CAGGACACCG TTCATGTTGC AATCGCCCCT TGGGCTCCAA GCCAAACCCC CGAGGCTAAC
    AATTGGTCCA TTGTACCCGT CATTCGATCT TCCACCGTTG CGCCGTCCAC TGTACAGTTC
    TCGCCCTCAT CGCTGGCCCT CCAGTCTTTC GCGACACACC TCCAGCAGGC TGCGCCCTCA
    AAGCTCTCCG GCCACAACAA AGGTGGCATT GAGATCCTGA TCTTGGATGT TTCGCCCATC
    GAGCTCGATA CCGTCTTTGT GAACCTTGAC GGAGAATTGG CTAAGAGACT GGAAGATGGG
    GAGGGAACCT TCTTCCGCGA ACACCCAGTC AACGGAAAGG GCAAGGCTCG TGCCGATGCT
    ACCCCTGAGG AACACTTGAC AGCTGCTTTG CGCACTGCCC TCGGTACCCT AAAGATTGTG
    CATTCTGGCG ATTTCTTCCC ATTGCCACTA CCACCTCACC CAGTTACCCA CCAACCTCCA
    AACCCAGGAC GGATAACGCT TTGCGAACCT GTGGCTCAGG GTATCCTCGC CCCTACTACA
    AAGATCATCG TGTCGAGAGT GCGCACCAGC AAGACCAAAC GTAGCTCCGC ATCTGGCCAG
    CAACAGGGGC GTAAACTGAA CGGAGTGGCC GAAGACGACG AAGACGCCTA CTTCTCGGCT
    GCCGAGGAAG ACCGCGACAG CAAGACCAAT GCCCAAACGG CTGAAACAGA TGCAGACGAT
    ACCGAATTCG AGCCACAGGC TGGAGAAGAT GAGGACAACT TGTCGGATGA TTCATTAGAC
    GAAATAATAT CCCTTCAAGT TCCTACTCTA CCCACTACGG TTACCGGCCA ATCTACAATA
    GGAACGGGCA CCCCGACGAT GCTCAGGGGC AGGAAGACCA ACGGTCCTGG GTCGGTTATC
    TCTAGTTATA CTGCCACCAC CGCCAGACCA GACCGACCCC GTGGCCGGCT CTTCAAGGCG
    CATGGATTAA TGAAACCTAT CCCACCTGAT CTGCTCCACC CTAAACCTAC ACCCGAAGAC
    GACGAAGAAG CGTGCATCTA CGTGGATATG AGGGATCTCA CCAGGGTGGG CTGTTTCTCT
    GGAGACTGGA TACGAGTCGA GGCGGCCTCT GAACCGCCCT CCAACGGGCT CGGCGCGTTT
    GGTTTGGGAA GCTTCGTGGA ACAGGAAGCA GACGAGATTA ACTGGAGACC CGCGAGGGTT
    TTCGGCCTGC CTGAAGGGTA TTCGAGCCGT TCAGCGACCA GGTCACACCA CTCTAGACAT
    GATAGCCGGA ACTCGTCCTT CTTTGAGGCA CAGTCCCAAA AGTCGAATCC ACCGGCTTAT
    ACTTCGCCTA TTCTTCTCGC AAACTTAGAC AACCCTCCCT ATCTTCGTCT ATCACCGATC
    AAGCGCCCTT CGAGCTCCAT CATTAAGGGC GTTGCTGCTC AGTCAAAGAC CAGCTCAACC
    TTGAAGCCAC CATTTGCTCG CGAGGTCATC CTACAACATG TGAGAACACC CACAGCGGTC
    GAGAGGGACG TACAGTCTGC GGTCATGGCT GGCCTGAAAC ACTATTTTGA GAGGAGATTG
    CGGGTTCTCA GGACTGGAGA TCTCATTGCT GTACCCATTG ACACCCAGTT GGGCAAGGCT
    CTCCAGGAGA GTACAATCCC TGGTGAGGAT TCTGCCATCG ATGAGGTCTT GGGTCTGATA
    GCTGCAACAC GTCCCGGACA GAGCCTACAT TATGACGATG TTGCCTGGTT CAGGGTTGGT
    CACGTCCAGG CCATGAAGCA AGACACCACA GAAGCAGTCG ATGGCGAGGA GGCAGAGGAT
    CTATGGGGCG GAATCGCCTG TGTAGATATC TCGCTCACAC ATTTAGAACA GTCAGGATCT
    GTCACGGGCC GCATTCCGGG CACGATCTCC AACAGCTGGC AGTATTACCT GGGTATCAGG
    AAGCTTCCTA AGCACCAGCA AAACCCAGGG CTACCTGCTC AACTGCAAAC GCTAGAGCCC
    GAGAAGCAGT ATGTCTCATC ACTCCGAAGG AAACTTCGCG AGCTCATGGC TGCCGCCACC
    AGTAAACCCG CGCTTCATCT CAACCTACCA CCACTGGCCA TTCTTCTTGT GTCGACGCAA
    AGGCATATCG GCAAAGCAGC CACAGCCATG CAAGCCTGTT CAGATATTGG TCTGCACACA
    TTTGCCATAG ACGCTTATGA CATTGTGAAT GATGGCGGTG GTGGAGGTGG TAGTGATGTC
    AAGACAGCTG GGTTCTTGAC ATCGCGCGCT GAGCGTGCTA TGAGCTGTGG TCCCGAATCA
    TGCGTCCTCC TGGTCCGCCA CATTGAAGCC CTTACTGCCG ACCGCATGGT TTCTTCCATC
    AAGGAGATCT TGGCCGACGC TAGGGTTTTG ATCGCCACCA CCACCGAGGT TGAGAAGGTT
    CCCGACGGCA TCCGTGCCCT CTTTACACAC GAGTTGGAGA TGAGTGCCCC CGACGAGCAA
    GAGAGAGAGG GTATCCTCAG CTCCATCCTC GCCGACCGCG GCATCGGTCT TGACCATGGG
    GTAGATCTCA GCGGCATCGC CCTCAAGACT GCCGCGCTCG TAGCAGGCGA TCTCGTCGAC
    GTTGTTGACC GTGCCTTGGT TGCGCAGCGA TCCCGCCTCG AGAAGCTCAC CGCCAAAGCG
    ACCGGCAGCA TCACCTTCCG TGACGTTCAG CTCGCCGGTG GCCCAGCCGC CTCTGGCCTG
    ACCAAGCAGG ATTTTGAGTT AGCCGTCGAT GCCGCCCGCA AGAACTTTGC TGATTCCATC
    GGCGCGCCGA AGATCCCCAA CGTCACATGG GACGACGTCG GTGGCCTAGG CAACGTCAAA
    GACGCCATCA CCGAGACCAT CCAGCTTCCG CTTGAGCGCC CCGAGCTGTT CGCCAAGGGC
    ATGAAGAAGC GCTCCGGTAT CCTGTTTTAC GGACCTCCTG GTACCGGCAA GACGCTCCTG
    GCCAAGGCCA TCGCGACCGA GTACAGCCTC AACTTCTTCA GCGTCAAGGG TCCCGAGCTG
    TTGAACATGT ACATTGGTGA GTCCGAAGCC AACGTGCGGA GGGTGTTCCA GCGTGCCCGC
    GACGCCCGGC CTTGCGTCGT CTTCTTCGAC GAGCTGGACT CCGTCGCCCC CAAAAGAGGA
    AATCAGGGCG ACAGCGGCGG TGTCATGGAT CGTATCGTGT CGCAGCTGTT GGCGGAGCTC
    GACGGCATGT CGGGCGGCGA AGGCGGAGGC GGCGGTGTGT TCGTCATCGG TGCGACCAAT
    AGACCTGATT TGCTGGATCC TGCACTGCTG AGACCCGGTC GCTTCGATAA GATGTTGTAC
    CTGGGTGTCT CGGACACACA CGACAAGCAG GTAACTATCA TGGAGGCTTT GACTAGAAAA
    TTCACTCTCC ACCCAACCGT CTCCCTCCGC TCTGTCGCCG AGCGTCTCCC TTTCACCTAC
    ACCGGCGCCG ACTTCTACGC CCTCTGCTCT GACGCCATGC TCAAGGCCGT TACCCGCCAG
    GCCACCTTGG TCGACACCAA GATCCGCGAG CTCAACGCCG CCGCCGGTCC CGAGGGAAAA
    CAGATCTCGA CTGCCTACTT CTTTGATCAT TATGCCACCA AGGAGGACAT CAGCGTCATG
    GTAACCGAGC AAGACTTTTT GGATGCCCAC AGGGAACTGG TGCCCTCCGT CAGTGCGGGC
    GAGTTGGAGC ACTATGAGCA GGTTAGGGCT ATGTTTGAGG GGGCTAAGGA TAAGGACAAG
    AAGAAGGAGG GTGCTGGTGG TGATGGCAAC GGAGTGGATG GACTTGAGAG CGGTAATAGG
    AATGGGATGC TGGCTATTAC AATGGGTGAT GACGGCGCTG GAGCTGAATC GACGAAGAAG
    GACGGCAAAG GAAAGGGAAA GGCGGCTGAT AACGAGGTCA ATGGTGCTGG TGAGGGGAAG
    GGCAAGGAAG GCGTTTCGCC TTTCCAGGAA TCGGGAGGAG ATGAGGATGA GGGGTTATAT
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B