NCU00743 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU00743
Entrez Gene ID	3880546
Full Name	glycogen debranching enzyme
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_959303.3	XP_964396.3	glycogen debranching enzyme

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Agl	NP_001074795.1
Bos taurus (cattle)	AGL	NP_001179400.1
Homo sapiens (human)	AGL	NP_000635.2
	AGL	XP_001106287.1
Canis lupus familiaris (dog)	AGL	NP_001041561.1
Rattus norvegicus (Norway rat)	Agl	NP_001102034.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001200	XP_321957.3
Caenorhabditis elegans (roundworm)	agl-1	NP_496984.1
Drosophila melanogaster (fruit fly)	CG9485	NP_726062.2
Magnaporthe oryzae (rice blast fungus)	MGG_00063	XP_003719078.1
Xenopus tropicalis (tropical clawed frog)	agl	NP_001072609.1
Pan troglodytes (chimpanzee)	AGL	XP_001159352.1
Danio rerio (zebrafish)	agla	NP_001166124.1
Saccharomyces cerevisiae (baker's yeast)	GDB1	NP_015510.1
Eremothecium gossypii	AGOS_AEL276C	NP_984584.1
Neurospora crassa	NCU00743	XP_964396.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU00743 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU00743 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf12807	XM_959303.3 Latest version!	Neurospora crassa OR74A glycogen debranching enzyme (NCU00743), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU00743 ( XM_959303.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_964396.3 Neurospora crassa OR74A glycogen debranching enzyme (NCU00743), mRNA.

Price & Availability↑

CloneID	ONf12807
Clone ID Related Accession (Same CDS sequence)	XM_959303.3
Accession Version	XM_959303.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	glycogen debranching enzyme
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_959303.2.

ATGATCCCCA GAACCATGGT TTCCAACGAA GTTTACCTAT TGCCGCTGAA GGACGATGGC 
TCGCCTGACG TGCCTGGCGA GTACATTTAC ATTGCGCCAA AGTCGAAGGA TCCAGTAACC 
ATTCGCTTTG CCATCGAGGG CACTTCCAGC ATCTGCAGCC ATGGCAGCTT GTGGGTGAAC 
ATCCCTGAGC ACGGCGAGCA GTTCCAGCGG AACAAGTTCC GCGAGTTCAA GCTCGTCCCT 
GACTTCAACC GCACACTCGA GATTTCGATC CCGATTTACG AGGCTGGCGC GTACGCCTTC 
TACACCACAT ATGCCGAGCT CCCTGATTTG GCCAGCAACC TGGTCAACGC CGACGGCACC 
GTGACCAAGG CTACTCAGAA AAAGACACCG TTGTACTATA TCGATGTCGC ACCCCGGCTC 
TCGCTGGACG GGCAGCCGCT GCCACTTCCG GCGCTCTCCA TCTTCTCCAT CATCAGCAAG 
TTCATGGGCA AGTATCCAAC AGACTGGGAG CGCCATCTTC GGGGAATCAG CGACCGAGGT 
TACAACATGA TCCACTTCAC ACCGTTGCAG GTACGAGGTG CATCAAATTC GCCATACAGC 
TTGTATGATC AATTGGGCTG GGACCCAGCT TGCTTCCCCG CAGGCGAGCC GGATGTCCAG 
AAGATGGTGG AAAGTCTGGA GAAGAACCAC TCCCTGCTCA GCTTGACGGA CATTGTCCTC 
AATCACACTG CACACAACAG CGAGTGGCTC TTGGAGCATC CCGAGGCCGG ATACAACTTG 
ACGACTGCGC CATGGCTCGA GTCCGCTTAC CTGCTCGACA CCAAGCTGCT CGAGCTGGGC 
ACCAGGTTGG AGGAGCTTGG CCTTCCCACC GAGCTCAAGG ACGTCGATGA TCTGGTCAAG 
ATTATGGATG CCATCAAGAA AGAGGTAATT GCCGAGATTC GTCTCTGGGA GTACTATACT 
CTTGATGTGG AGCGCGATGC CGATGCCGCG GTTAAGTCCT GGGCTGCCAA CGACATTGAC 
TTCCCACAGG CCTCCGTTGG AGCTGGAGGT ATCGACTCTC TACACTCCGC TACCCCTAAG 
GAGCAGGCAG ATTTCTTGAT CCAGCATGGT CTGCAGAACA TGGATTATCT TGGTGAGCGC 
TTCAGGCGGA GGGCTAACCC CAAGGTGGCT GCCGCTCTTT TGTCTGCTAT CTTCGGCCGG 
TACGAAGGAG AGACAAAGAA TACTGCCGAT CAGGCTGCAG CCAGAACCAA GATTGTCGAG 
ATTCTGGAGA TCGTCAACGT TCCATTCTAC AAGGAATATG ATGACGAAGT TGCCGAGATC 
CTGCAGCAGC TGTTCAATCG CATCAAGTAT GTCCGGCTCG ATGACCACGG CCCGAAGCTC 
GGGCCCATCA ACGCCGAGAA TCCTCTGATC GAGACGTACT TCACTCGTCT TCCCGTCAAC 
GAGAAGACGA AGAAGCACAA GAAGGAAGAC CTGGTTCTCG CAAACAACGG ATGGGTATGG 
GGCGGAAACG CGCTGGTCGA CAATGCCGGT CCCGATTCTC GTGTCTACCT TCGCCGAGAA 
GTCATTGTCT GGGGAGACTG CACAAAGCTC CGCTACGGAA GCGGTCCTGA AGACAGCCCA 
TGGCTTTGGG AGCACATGAC CAAGTACGCC CGCATGCTGG CCAAATACTT TGCCGGCTTC 
CGTATTGACA ACTGCCATTC CACGCCTCTT CATGTCGCTG AGCACATTTT GGATGAGGCT 
CGTCGTGTTC GCCCCGATCT CTATGTCGTT GCGGAGCTTT TCACCGGCTC CGAAGAGATG 
GATTACGTCT TTGTCAAGCG TCTGGGTATC AGCGCACTCA TTCGTGAGGC CATGCAAGCA 
TGGAGCACTG GCGAGCTCAG TCGCCTGGTT CATCGTCACG GAGGTCGACC TATTGGCAGC 
TTTGAAGTTG ACGAGGTATC AAGCAACGAG GGCCGCTCGT CGTCCATCAG CGGCACGAAT 
GGGGATGGCG TCTACACACG CGAGGTCATC CGCCGTATCA GGCCGGTTCC CGTACAGGCA 
CTATTCATGG ACTGCACTCA TGACAATGAG GTTCCTGCTC AGAAGCGCGA TGCGCGTGAC 
ACGCTTCCAA ACGCAGCCCT GGTTGCCATG TGCGCTAGCG CTACCGGCAG CGTGATGGGA 
TACGACGAGA TCTATCCTAA GCTTGTTGAC TTGGTTGGCG AAACCCGCCT TTACACGTCC 
GAAGCTTCCA AAGCTCCTGT TAAAACGGGA GAGGGCAAGG ATGGAATTGC TGGTGTGAAG 
AAGCTTCTCA ACCAGATCCA CACCTTGATG GGTATGGATG GCTATGATGA GACGCACATT 
CACCATGAGG ACGAGTACGT CACTGTCCAC AGAGTCCATC AGGAGTCAAG AAAGGGCTAC 
TTCCTCATCG CACACACTGC GTTCCCCGGT TATGGAAACG GCAATGGCGC TTTCAACCCT 
GTCCACCTGA CCGGTACAAA GGCTCGCCAT CTCGGTAGCT GGATGCTCGA GGTTGATGCC 
AGCAAGGAAG CTGTTGAGGA GGTCCTGAGC GACAAGAAGC ATCTTCGTGG TCTTCCCAGC 
CGTCTAGTAG GTTTGCCAGG CGTGCGGATG GAAGTCAAGG GCCAGGATAC CATTATCACC 
GTCCGCGAGA AGTTCCCTCC TGGAAGTATT GCTCTCTTTG AGACTTGGAT CCCGGCGGCA 
GAACACTCGA GTGGTTTGGA CAACTTTGTC ACCTCTGGCG CAAAGGCAGC AATGGATGAG 
CTTGATCTTG TCGATCTCAA CTTCCTTCTG TACAAGTGCG AGCCTGAGGA GCGTGATGCT 
AGCGAAGGCC AGGATGGAAC CTACGACATC CCTGGCCACG GCAAGATTGT GTACGCTGGA 
TTGCAGGGTT GGTGGAGCAT TCTGAAGGAT GTCATCAAGG ACAACAATCT CGCCCATCCT 
CTATGCCAGC ATCTCCGAGA CGGCCAGTGG GCTCTTGACT ACATTGTTGG GAGACTTGAG 
CGTGCCTCGA AGAAAGACGA CTTTCGCCGG CTGGCGAAGC CTGCTCAATG GTTGAAGGAG 
CGCTTTGATG CTATTCGTCC CATCCCAAGC TTCTTACTTC CTAGATACTT CGGTCTTGTG 
CTTCGCACAG CCTATAACGC GGCCTTTGAA CGCGGTATCT CCCTTATGAA CAACAATGTC 
ATAAAGGGGC AATGGTTCCT GCAGAGTTTG GCCATGGTTA GCGTTCAGAT GACTGGTCTG 
GTAAAATCAG CGTCATTGTA TCCTAACCGG CTCGTGCCGT CGCTTGCTGC GGGTCTTCCT 
CACTTCGCTG TTGAGTGGGC TCGCTGCTGG GGTCGCGATG TGTTCATTTC TCTCCGCGGC 
TTGTACCTCG GTACTGGTCG TTACGCAGAA GCCAGAGAGC ACATCCACGC CTTTGCCAGT 
GTGCTCAAGC ATGGCATGAT CCCTAATCTT CTAGGAAGCG GCAATAACCC CAGATACAAC 
TCTAGAGATT CTGTCTGGTT CTTCCTGCAG TGCATCCAGG ACTACACCCG TCTGGTTCCT 
GATGGCTTGT CGATCCTAGA CGACAAGGTC AAGCGCCGTT TCCTCCCTTA CGATGACACG 
TACTTTGACG TCGACGATGA GCGTGCGTAC AGCAAGGAAA GCACCATCGC CGAGATCATC 
CAGGAAGTTT TCCAGCGCCA TGCCGAGGGC ATGAAGTACC GTGAGGCCAA CGCCGGCCCC 
AACTTGGATA TGCAGATGAG CGATGCCGGT TTCAACCAGG AGATCAAGGT TGACTGGAGC 
AACGGTTTCA TCTTCGGCGG TAATCAAGCC AACTGCGGTA CCTGGATGGA CAAGATGGGC 
GAGAGCGAGC GTGCTGGTTC CAAGGGTGTT CCAGGAACTC CCAGAGACGG TGCCGCCGTT 
GAGATCACAG GTATGCTGTA CAGCAACCTG AAGTGGGCAG CTTCTCTCAA CGAAGCCGGC 
AAATTCAAGC ACTCTAGTGT TCGCAAGGCC GATGGTACCG AAATCACTCT CAAGGACTGG 
GCTGCTCTCA TCAAGGACAA CTTTGAGCGT TGCTACTTTG TCCCCATCTC CCCCGACGAG 
GACTCCAAGT ACGATGTCAA CCCGGCGATC ATCAACCGTC GTGGCATCTA CAAGGATCTC 
TACCGTTCCG GTAAGGAGTA TGAAGACTAC CAGCTCCGCT GCAACTTCCC CATTGCCATG 
ACATGCGCCG CCGACCTCTT TGTTCCGGAG CACGCTATGC ATGCTCTCTG GGTTGCCGAC 
GCCGCTCTCC GTGGTCCCAC TGGTATGGCT ACGCTCGACC CCAGCGACAT GAACTACAGA 
CCGTACTACA ACAACAGCGA GGACAGCGAC GACTTTGCCA CCAGCAAGGG TCGCAACTAC 
CACCAGGGTC CTGAGTGGCT GTGGCCGACT GGTTTCTTCC TCCGGGCGCT GCTCAAGTTT 
GACTTGATGC GCCGCGGCCG TGACGATGCC GAGGGCCGCA CTGAGGCGTT CCAGCAGGTC 
ACCAGGCGAC TTATAGGATG CAAGGAGATG ATTCAGCGGA GCCCATGGGC TGGACTTACG 
GAGTTGACGA ACAAGAATGG AGAGGAGTGT CATGATTCGT CTCCTACCCA AGCGTGGTCC 
GCCGGTTGCT TGATCGACCT CTACATGGAC GCTGCTGAGG AACAAGCCAA GCTTGAGAAG 
CACTCGCTTC CCTTGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_964396.3
CDS	649..5349
Translation	MIPRTMVSNEVYLLPLKDDGSPDVPGEYIYIAPKSKDPVTIRFAIEGTSSICSHGSLWVNIPEHGEQFQRNKFREFKLVPDFNRTLEISIPIYEAGAYAFYTTYAELPDLASNLVNADGTVTKATQKKTPLYYIDVAPRLSLDGQPLPLPALSIFSIISKFMGKYPTDWERHLRGISDRGYNMIHFTPLQVRGASNSPYSLYDQLGWDPACFPAGEPDVQKMVESLEKNHSLLSLTDIVLNHTAHNSEWLLEHPEAGYNLTTAPWLESAYLLDTKLLELGTRLEELGLPTELKDVDDLVKIMDAIKKEVIAEIRLWEYYTLDVERDADAAVKSWAANDIDFPQASVGAGGIDSLHSATPKEQADFLIQHGLQNMDYLGERFRRRANPKVAAALLSAIFGRYEGETKNTADQAAARTKIVEILEIVNVPFYKEYDDEVAEILQQLFNRIKYVRLDDHGPKLGPINAENPLIETYFTRLPVNEKTKKHKKEDLVLANNGWVWGGNALVDNAGPDSRVYLRREVIVWGDCTKLRYGSGPEDSPWLWEHMTKYARMLAKYFAGFRIDNCHSTPLHVAEHILDEARRVRPDLYVVAELFTGSEEMDYVFVKRLGISALIREAMQAWSTGELSRLVHRHGGRPIGSFEVDEVSSNEGRSSSISGTNGDGVYTREVIRRIRPVPVQALFMDCTHDNEVPAQKRDARDTLPNAALVAMCASATGSVMGYDEIYPKLVDLVGETRLYTSEASKAPVKTGEGKDGIAGVKKLLNQIHTLMGMDGYDETHIHHEDEYVTVHRVHQESRKGYFLIAHTAFPGYGNGNGAFNPVHLTGTKARHLGSWMLEVDASKEAVEEVLSDKKHLRGLPSRLVGLPGVRMEVKGQDTIITVREKFPPGSIALFETWIPAAEHSSGLDNFVTSGAKAAMDELDLVDLNFLLYKCEPEERDASEGQDGTYDIPGHGKIVYAGLQGWWSILKDVIKDNNLAHPLCQHLRDGQWALDYIVGRLERASKKDDFRRLAKPAQWLKERFDAIRPIPSFLLPRYFGLVLRTAYNAAFERGISLMNNNVIKGQWFLQSLAMVSVQMTGLVKSASLYPNRLVPSLAAGLPHFAVEWARCWGRDVFISLRGLYLGTGRYAEAREHIHAFASVLKHGMIPNLLGSGNNPRYNSRDSVWFFLQCIQDYTRLVPDGLSILDDKVKRRFLPYDDTYFDVDDERAYSKESTIAEIIQEVFQRHAEGMKYREANAGPNLDMQMSDAGFNQEIKVDWSNGFIFGGNQANCGTWMDKMGESERAGSKGVPGTPRDGAAVEITGMLYSNLKWAASLNEAGKFKHSSVRKADGTEITLKDWAALIKDNFERCYFVPISPDEDSKYDVNPAIINRRGIYKDLYRSGKEYEDYQLRCNFPIAMTCAADLFVPEHAMHALWVADAALRGPTGMATLDPSDMNYRPYYNNSEDSDDFATSKGRNYHQGPEWLWPTGFFLRALLKFDLMRRGRDDAEGRTEAFQQVTRRLIGCKEMIQRSPWAGLTELTNKNGEECHDSSPTQAWSAGCLIDLYMDAAEEQAKLEKHSLPLR

Target ORF information:

RefSeq Version	XM_959303.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A glycogen debranching enzyme (NCU00743), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_959303.3

ORF Insert Sequence:

ATGATCCCCA GAACCATGGT TTCCAACGAA GTTTACCTAT TGCCGCTGAA GGACGATGGC 
TCGCCTGACG TGCCTGGCGA GTACATTTAC ATTGCGCCAA AGTCGAAGGA TCCAGTAACC 
ATTCGCTTTG CCATCGAGGG CACTTCCAGC ATCTGCAGCC ATGGCAGCTT GTGGGTGAAC 
ATCCCTGAGC ACGGCGAGCA GTTCCAGCGG AACAAGTTCC GCGAGTTCAA GCTCGTCCCT 
GACTTCAACC GCACACTCGA GATTTCGATC CCGATTTACG AGGCTGGCGC GTACGCCTTC 
TACACCACAT ATGCCGAGCT CCCTGATTTG GCCAGCAACC TGGTCAACGC CGACGGCACC 
GTGACCAAGG CTACTCAGAA AAAGACACCG TTGTACTATA TCGATGTCGC ACCCCGGCTC 
TCGCTGGACG GGCAGCCGCT GCCACTTCCG GCGCTCTCCA TCTTCTCCAT CATCAGCAAG 
TTCATGGGCA AGTATCCAAC AGACTGGGAG CGCCATCTTC GGGGAATCAG CGACCGAGGT 
TACAACATGA TCCACTTCAC ACCGTTGCAG GTACGAGGTG CATCAAATTC GCCATACAGC 
TTGTATGATC AATTGGGCTG GGACCCAGCT TGCTTCCCCG CAGGCGAGCC GGATGTCCAG 
AAGATGGTGG AAAGTCTGGA GAAGAACCAC TCCCTGCTCA GCTTGACGGA CATTGTCCTC 
AATCACACTG CACACAACAG CGAGTGGCTC TTGGAGCATC CCGAGGCCGG ATACAACTTG 
ACGACTGCGC CATGGCTCGA GTCCGCTTAC CTGCTCGACA CCAAGCTGCT CGAGCTGGGC 
ACCAGGTTGG AGGAGCTTGG CCTTCCCACC GAGCTCAAGG ACGTCGATGA TCTGGTCAAG 
ATTATGGATG CCATCAAGAA AGAGGTAATT GCCGAGATTC GTCTCTGGGA GTACTATACT 
CTTGATGTGG AGCGCGATGC CGATGCCGCG GTTAAGTCCT GGGCTGCCAA CGACATTGAC 
TTCCCACAGG CCTCCGTTGG AGCTGGAGGT ATCGACTCTC TACACTCCGC TACCCCTAAG 
GAGCAGGCAG ATTTCTTGAT CCAGCATGGT CTGCAGAACA TGGATTATCT TGGTGAGCGC 
TTCAGGCGGA GGGCTAACCC CAAGGTGGCT GCCGCTCTTT TGTCTGCTAT CTTCGGCCGG 
TACGAAGGAG AGACAAAGAA TACTGCCGAT CAGGCTGCAG CCAGAACCAA GATTGTCGAG 
ATTCTGGAGA TCGTCAACGT TCCATTCTAC AAGGAATATG ATGACGAAGT TGCCGAGATC 
CTGCAGCAGC TGTTCAATCG CATCAAGTAT GTCCGGCTCG ATGACCACGG CCCGAAGCTC 
GGGCCCATCA ACGCCGAGAA TCCTCTGATC GAGACGTACT TCACTCGTCT TCCCGTCAAC 
GAGAAGACGA AGAAGCACAA GAAGGAAGAC CTGGTTCTCG CAAACAACGG ATGGGTATGG 
GGCGGAAACG CGCTGGTCGA CAATGCCGGT CCCGATTCTC GTGTCTACCT TCGCCGAGAA 
GTCATTGTCT GGGGAGACTG CACAAAGCTC CGCTACGGAA GCGGTCCTGA AGACAGCCCA 
TGGCTTTGGG AGCACATGAC CAAGTACGCC CGCATGCTGG CCAAATACTT TGCCGGCTTC 
CGTATTGACA ACTGCCATTC CACGCCTCTT CATGTCGCTG AGCACATTTT GGATGAGGCT 
CGTCGTGTTC GCCCCGATCT CTATGTCGTT GCGGAGCTTT TCACCGGCTC CGAAGAGATG 
GATTACGTCT TTGTCAAGCG TCTGGGTATC AGCGCACTCA TTCGTGAGGC CATGCAAGCA 
TGGAGCACTG GCGAGCTCAG TCGCCTGGTT CATCGTCACG GAGGTCGACC TATTGGCAGC 
TTTGAAGTTG ACGAGGTATC AAGCAACGAG GGCCGCTCGT CGTCCATCAG CGGCACGAAT 
GGGGATGGCG TCTACACACG CGAGGTCATC CGCCGTATCA GGCCGGTTCC CGTACAGGCA 
CTATTCATGG ACTGCACTCA TGACAATGAG GTTCCTGCTC AGAAGCGCGA TGCGCGTGAC 
ACGCTTCCAA ACGCAGCCCT GGTTGCCATG TGCGCTAGCG CTACCGGCAG CGTGATGGGA 
TACGACGAGA TCTATCCTAA GCTTGTTGAC TTGGTTGGCG AAACCCGCCT TTACACGTCC 
GAAGCTTCCA AAGCTCCTGT TAAAACGGGA GAGGGCAAGG ATGGAATTGC TGGTGTGAAG 
AAGCTTCTCA ACCAGATCCA CACCTTGATG GGTATGGATG GCTATGATGA GACGCACATT 
CACCATGAGG ACGAGTACGT CACTGTCCAC AGAGTCCATC AGGAGTCAAG AAAGGGCTAC 
TTCCTCATCG CACACACTGC GTTCCCCGGT TATGGAAACG GCAATGGCGC TTTCAACCCT 
GTCCACCTGA CCGGTACAAA GGCTCGCCAT CTCGGTAGCT GGATGCTCGA GGTTGATGCC 
AGCAAGGAAG CTGTTGAGGA GGTCCTGAGC GACAAGAAGC ATCTTCGTGG TCTTCCCAGC 
CGTCTAGTAG GTTTGCCAGG CGTGCGGATG GAAGTCAAGG GCCAGGATAC CATTATCACC 
GTCCGCGAGA AGTTCCCTCC TGGAAGTATT GCTCTCTTTG AGACTTGGAT CCCGGCGGCA 
GAACACTCGA GTGGTTTGGA CAACTTTGTC ACCTCTGGCG CAAAGGCAGC AATGGATGAG 
CTTGATCTTG TCGATCTCAA CTTCCTTCTG TACAAGTGCG AGCCTGAGGA GCGTGATGCT 
AGCGAAGGCC AGGATGGAAC CTACGACATC CCTGGCCACG GCAAGATTGT GTACGCTGGA 
TTGCAGGGTT GGTGGAGCAT TCTGAAGGAT GTCATCAAGG ACAACAATCT CGCCCATCCT 
CTATGCCAGC ATCTCCGAGA CGGCCAGTGG GCTCTTGACT ACATTGTTGG GAGACTTGAG 
CGTGCCTCGA AGAAAGACGA CTTTCGCCGG CTGGCGAAGC CTGCTCAATG GTTGAAGGAG 
CGCTTTGATG CTATTCGTCC CATCCCAAGC TTCTTACTTC CTAGATACTT CGGTCTTGTG 
CTTCGCACAG CCTATAACGC GGCCTTTGAA CGCGGTATCT CCCTTATGAA CAACAATGTC 
ATAAAGGGGC AATGGTTCCT GCAGAGTTTG GCCATGGTTA GCGTTCAGAT GACTGGTCTG 
GTAAAATCAG CGTCATTGTA TCCTAACCGG CTCGTGCCGT CGCTTGCTGC GGGTCTTCCT 
CACTTCGCTG TTGAGTGGGC TCGCTGCTGG GGTCGCGATG TGTTCATTTC TCTCCGCGGC 
TTGTACCTCG GTACTGGTCG TTACGCAGAA GCCAGAGAGC ACATCCACGC CTTTGCCAGT 
GTGCTCAAGC ATGGCATGAT CCCTAATCTT CTAGGAAGCG GCAATAACCC CAGATACAAC 
TCTAGAGATT CTGTCTGGTT CTTCCTGCAG TGCATCCAGG ACTACACCCG TCTGGTTCCT 
GATGGCTTGT CGATCCTAGA CGACAAGGTC AAGCGCCGTT TCCTCCCTTA CGATGACACG 
TACTTTGACG TCGACGATGA GCGTGCGTAC AGCAAGGAAA GCACCATCGC CGAGATCATC 
CAGGAAGTTT TCCAGCGCCA TGCCGAGGGC ATGAAGTACC GTGAGGCCAA CGCCGGCCCC 
AACTTGGATA TGCAGATGAG CGATGCCGGT TTCAACCAGG AGATCAAGGT TGACTGGAGC 
AACGGTTTCA TCTTCGGCGG TAATCAAGCC AACTGCGGTA CCTGGATGGA CAAGATGGGC 
GAGAGCGAGC GTGCTGGTTC CAAGGGTGTT CCAGGAACTC CCAGAGACGG TGCCGCCGTT 
GAGATCACAG GTATGCTGTA CAGCAACCTG AAGTGGGCAG CTTCTCTCAA CGAAGCCGGC 
AAATTCAAGC ACTCTAGTGT TCGCAAGGCC GATGGTACCG AAATCACTCT CAAGGACTGG 
GCTGCTCTCA TCAAGGACAA CTTTGAGCGT TGCTACTTTG TCCCCATCTC CCCCGACGAG 
GACTCCAAGT ACGATGTCAA CCCGGCGATC ATCAACCGTC GTGGCATCTA CAAGGATCTC 
TACCGTTCCG GTAAGGAGTA TGAAGACTAC CAGCTCCGCT GCAACTTCCC CATTGCCATG 
ACATGCGCCG CCGACCTCTT TGTTCCGGAG CACGCTATGC ATGCTCTCTG GGTTGCCGAC 
GCCGCTCTCC GTGGTCCCAC TGGTATGGCT ACGCTCGACC CCAGCGACAT GAACTACAGA 
CCGTACTACA ACAACAGCGA GGACAGCGAC GACTTTGCCA CCAGCAAGGG TCGCAACTAC 
CACCAGGGTC CTGAGTGGCT GTGGCCGACT GGTTTCTTCC TCCGGGCGCT GCTCAAGTTT 
GACTTGATGC GCCGCGGCCG TGACGATGCC GAGGGCCGCA CTGAGGCGTT CCAGCAGGTC 
ACCAGGCGAC TTATAGGATG CAAGGAGATG ATTCAGCGGA GCCCATGGGC TGGACTTACG 
GAGTTGACGA ACAAGAATGG AGAGGAGTGT CATGATTCGT CTCCTACCCA AGCGTGGTCC 
GCCGGTTGCT TGATCGACCT CTACATGGAC GCTGCTGAGG AACAAGCCAA GCTTGAGAAG 
CACTCGCTTC CCTTGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU00743 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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