qde-3 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	qde-3
Entrez Gene ID	3880179
Full Name	quelling-defective-3
Synonyms	mus-19,rec-6
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_958937.3	XP_964030.3	RecQ family helicase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following qde-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the qde-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf12650	XM_958937.3 Latest version!	Neurospora crassa OR74A RecQ family helicase (qde-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

qde-3 ( XM_958937.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_964030.3 Neurospora crassa OR74A RecQ family helicase (qde-3), partial mRNA.

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CloneID	ONf12650
Clone ID Related Accession (Same CDS sequence)	XM_958937.3
Accession Version	XM_958937.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	RecQ family helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_958937.2. COMPLETENESS: incomplete on the 5' end.

ATGGCGGAAC GGGAACGGGA AGGAACGAGT TCGAGTAGTA GTAGTAGTAC TAGTAGAGGT 
ACTAGTAGAG GTACTACCCC ACTGTCTCTG TCGCCGCCGC CGCAGGAGCA GGAGCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA GAGCAGCAGA ACACGTCTGA CACGCGATCC 
AATCAGTTGT CTTCGTCGTT GAGGACTTTT ACTACTTCTG CGACTTCTGC GACTTCCTCG 
TCGGTGCAGC AGAATACCTC TAACCACCAG GTGCTGCTGC CGCTGCCTTC CACGTCTTCG 
TCTCCTTCCA CGTCTACGTC TTTCAGTTCG TCGCTGTCGT CGTGGGTGCC TCATTCCCTA 
AAAGTTCTTT ACCATTTCAC TGCCGGCTCA GTTACTGCTG CTGCCTCTAC TAGTAGTAGT 
AGCAGTAATA GTGGTGGTGA TACAGTGCTA AATCCCAATG CGTCCGGCGG CGTGCTCGGC 
CGGTTGCTCA CCAGTTCGAC AGCGCAATCC ACGCTCATGA CGCGCAACAA TTGGGGGGAG 
CAAGTTTCTT GGTTGAAGAG ATCGGGAAAT TCCATTGTCG TTCCACAGGG GCCCGTGTAT 
CCTTCTGCTG CTGCTTCGAT ACAGACTACG ACACCAACAT CAACAACAAC AACAACAACA 
CCAAGCGCGA TCGAAGACGG TTATTCCTCG ACGAGCAGTT TTCCACGAGA TAACAATTTA 
TTCCCCTTGT ATTCCACTCC GGCTGTCACC AATCCACAAG TCAGTGGAAG TGAAAGTCTT 
GGAGGAGCAG GAAGAGGAAC AGGAAGAGGA AGAGGAGGAG GAAGAGTGGG AGGAGGAGGA 
CCAGTCCAGC AGCAACAACA ACCACCACCA CTGGGACCAC CACCACCTCT ACCACCACCA 
CCACCACCAC CACCACCACA ACAACCAACT CAACAACGAA CTCAACAACC AACCCCAAAC 
CCAACCTCGA CCTCAACCTC AACCTCAACC CTTGCGACCT CGAGATACAC AAACACATCC 
TCGACAAATG ACGCCCGACC CGCTACACGC CAACAGATTG CCCCCGAGGT AGGAGCATCG 
ACGCATCAGG ATTCAGTTGG ACTTGGAGAA GGAGGAGGAG GAGGAATGGC GAAGCTCTCA 
GTCAAGAACA ACCTGCCACG GCCGCACTTG GTCTCCTTGT CGTCGTCAAC GACAGGCTCT 
GGGTCTGGTT CTGCGTCTAG GTCAGCTTCT GCTAAGCACG GAAGTGCCGG TTCCAGTACC 
TTTGATCATG AACAACATCA ACAACATCAA CAACAACAAC AACAAAAGCG CCAGCGGTCG 
CAATCAGAAG CACGACAACA GCAGCAGCAA CAGCAACAGC AACAGCAACA GCAACAACAA 
CAACAACAAG CACAGCACCA TGCACATTCT ACATATGCAC AAAGACCCCA ACCCACCCCC 
CAACAACGAC CACCCCAAAA CCTACTGACA CCTGCTTCAA CCACTGGTGC CAGCGTCGGC 
CCGCTCCAAC GCGCATACTC GGTTTCATTA GCTGCGAGAC AGTCCCCCTC GACAAACTTG 
GTCCGTCCAA AGACCGACTC GCCAGCTCCC CACACTTTAC ACCTCAAGAA CAAGAAGAAC 
CTCCGTCACC CCGCCCCCAC GCCCGACAGT CCGATCGTAG ACGACGATAT TTTCTCCGAC 
GCCGTCGATC TTACCGAAGA ACTCGATCAT GACCATGATC TCAACGGCAA AGACAAAGAC 
AACACCGACA ACGACAACAC AGTCGCTTCC AGTTCGCTAA TAGGGTTCGG CGATGACAAG 
TTACTGTGGC GAGAGGACTT TGCTGAGCGT GCAGAGCCCG AACATGAAAG AGGTGGGAGC 
AGGCCTCGCC AGGTCAAGAA ACGGAAGATA TCGAATGACT ACATTATGAA GGATGAGGAT 
GTCTCGCTTT TTGATGATGA TGGCGAGGAG GACGAGTTTA TGGATATCAA TGAGCTAGTT 
CAGGGGGATC GGGAAAGTAC TCCGAAGCCA AAGGCTACAT CGAGGTCTGT CTCGACGAGG 
CTGCCGCCTA CAGTATCGCT GCAACGGGGT CGGTCTCCTA AGAGGAAGGA GGCTTCAGTT 
GAAAAGCGCA CAACGGAAAA CCAGCAACAG GCTGACAGAG AAGACGAACC GTCGTTTATG 
TCAAGTCCAG ATGTCGACAA CTCCCGCAAG CGAAAGTCTT CTGGATCGCC CACAGGTTTA 
ACGACGCCAA GACCCCAGCA GAAGCAAACG GAAGAGGTCC CAGGTACGAC CACCGCCAAG 
AAGCCACGGC GCAGTGAAGT GATGGACTCG GAGGACGAGG CATTCACTCC TCTTTCTGCT 
GGGTCGCTGC CTGGGAGTGC GGAGTTCTTC AGAAGCGGTG GGACCACCAC ACGGGAATTG 
GGTTTGGACG AAGACACGGT TATGGACACG CCTAGTAGGC CACCGGTCGA GTCCACTTTG 
CCAACTCTCG AGTCTGTGGA AAGTCGACCA CCCCCCCTGC CGCCCATGGA TCTACCATCA 
CAGCGAAAAC CGCTAGAGCC GTTGAACACT CCGCGCAACC AGCTGCTTGA GTCGGTCGAA 
AGGCCAACAC AGCAGCCGTC GGTGGGGCCG AGTTTTGCAC AGAGTAGCAC ACTCGCCGAA 
AGCTCCCTGC CGCCGTCAAT GCCGCCGCCA AGTGAAGACC CCCTCAACAC CAGGGAGAAC 
AGCAACCTTG AGGAGTTCGA CTACAAGCTT TACAAACCCC TGCTAGATCT TTTCGTCAAC 
GCACCCGCAA TCTTGGAAAG AGAACTGAGC GCCGTTAATG ACGAGCTTCA GGAGAACATG 
ATCAAGCTGC GGGACTGTCT GCGCCTGCCC AGGGAAGAAA GAGACAGGGC ACGCGAAGAG 
GTGAAGAAGG AAAAGGAAAT GCTCAAGCGA CGGGACATTG CGCTCAGAGC CCTCCAGGAC 
GAACACAAGT TGTACGTCAA GAAACGCAAA GAGCATAATT TGATCAACGA GGAAATCGTT 
CGCGCTTATG CTGAAGAAGA CGATGAGTAC GAGGATCAGT TAATGGCGCA GCTGGACAAG 
TTGGATGATG AGGTTGAGGC TATCGTAAAG AGTCTGACAA GGCTTATTGT GGCGGCGGGG 
ATCACGGAGA AGAGCTTTGA CCTAAAGAAG GAGGAGGAAG AGGAGGAGGA GAAGCCGATC 
ATCATAGCGA CTCCGACACC TTCGACGAGG ACCGAGGCCC CGGTTCTGCC GACGACCGAG 
TATCATAATT CCCAGCAGGT CATATTGCAG ACTCAACATC CTGCTGCGCA GCAGGTTTCT 
CACCGGGTGC CACCACCTCC GACACCGAGT TTTCAAACGG CGCGCCAGAC TCCGGTGTCA 
TATCAGAGCA GACCGACCAA CAACTCCTTT CCTGATATCT CGGCGGAAGA AGCCATGATG 
TTCGATAAAG AAGACCCCTT CATGGAACAA CAGCACGCCC CGGCCTCTGC TCCCTTCCAG 
GCCACCCTTC CCCAGCGCAA CAGCCCTTTC AAAACCGCCC CGTTCAAGCC AGTCCACGGC 
CACGATTACT TTGACGATGA AGACGACGAT GCCGACCTCC TGGCAGCAGT AGACAGCGCC 
GAGACGTATA CTTCTACGGC CGCCACCACC ACCACCAACA ACAACAATCA CTTACGATCA 
CAATCGGTGA TGTCAACATC CACGGCGACC ACGATCAAAC CGAGGAAACG CAACGAAAAT 
GCCAATGCCA AGAAGGCCAA GTCCGTACAT GCAAAGTTAT CGATGCCGCC CGAAAAGATG 
AAGTATGCGT GGTCGAATGA TGTGAGGAAG GCTCTCAAGG ATAGGTTTCG GATGTCGGGG 
TTCAGACAGA ATCAGTTGGA GGCTATTAAT GCTACTTTGG GTGGTAAGGA TGCCTTTGTG 
TTGATGCCGA CTGGTGGTGG AAAGTCTCTG TGCTATCAGT TGCCGGCTGT AGTCAGGAGC 
GGCAAGACGC GTGGTATCAC AGTCGTCATC TCCCCTCTGC TAAGTCTGAT GCTGGATCAA 
GTCAACCATT TGGCAAACCT GATGATCCAA GCTTACGCTT TCAACGGAGA CATGAACTCA 
GAAATGCGCC GAATGGTGTT TCAGAAGCTT GATGCTGAGC ATCCTGAGCA TGAGCTCCAA 
CTGCTCTATG TCACCCCGGA GATGGTGAGC AAGAACCAGA CATTCGTCAA CAAGATGATG 
GACCTCTACC GGAGGAAAAA GCTGGCTAGA ATTGTTATCG ACGAGGCTCA CTGCGTCAGT 
CAATGGGGCC ATGACTTCCG ACCCGATTAC AAAGCTATAG GAGAGTTTCG TAAGAGGTTT 
CCCGGAGTTC CGGTCATGGC TTTGACAGCG ACGGCAACAC AGAACGTCAT CTTGGATGTC 
AAGCATAACC TGGCAATGGA GGACTGCCAG ACTTTCTCCC AGAGCTTTAA TCGGCCGAAC 
CTCTACTATG AGGTCAGGAT GAAGGAGCAG AATCTGATTG CCCGCATCGC AGAGTTGATC 
AAGGAGAAGT ATGACGGCCA GACGGGTATC ATCTACACAT TATCAAGAAA GAGTGCCGAG 
AACATCGCCA AAAATCTCCA GGAAAAACAC CGCATCAAAG CAAAGCACTA CCATGCCTCC 
ATCACCACCG ACGAAAAGAT CAGCGTTCAA CATGAATGGC AAACCGGCCG AGTCAAAGTC 
GTGGTAGCCA CCATTGCCTT CGGCATGGGC ATCGACAAGC CTGACGTCCG CTTTGTTATC 
CACCAGCACA TCCCCAAGTC GCTCGAAGGT TACTATCAAG AAACCGGCCG CGCCGGACGT 
GACGGCAAGC CATCGGACTG CTACTTGTAC TTTGCCTATG GCGACATTCA ATCCCTACGT 
CGTATGATCG CCGACGGCGA AGGTGACTAC GCGCAAAAGG AGCGTCAGCT ACAAATGCTC 
AACCGTGTGG TCAGCTATTG CGAGTCGCAG CACACGTGCC GGCGCGAAGA AGTGCTCCGC 
TACTTTGGCG AGGAGTTTGA CTACCGGAAG TGTAGAGACG GATGCGATAA CTGCCGGAAC 
GGACGCATCT CGAAGTCGAC GGAGATGAGG GATTTTACGG AAATCGCCTT CGCCGCGATC 
GAGGTGGTGA AGAGCCAGCA GCCCATCACG CTGGGCAAGC TGTGCGACAT CCTGATGGGC 
AAGAGAAAGA ACGAGCACGG TGGCGTGTGT CACTTTGGTA TCGCCAAGGG GAGCACGCAG 
AGGGAGCTGC AGAGGATCGT GCTGCAGCTG AATTTCCACA AGGCGCTGGG CGAGGACAAT 
ATCATGAATG GGGCGGGGAT GCCTATTACC TACTATATTA CCGGCCCTGA AGCTGGTGCT 
TACCTCTACA ATGGCAAGCG GTTGATGCTG CCAGTTCCCT CAAACAAGTC CGTCGAACCC 
CCGTCTCGGT CTAAGCAGCG GAGCCGTCGA GTCGATGAGG ATATGGATGA GCAAGAACTT 
CCCACCCTGC AACGACCGCC AACATCAACA AATGTCTCTT CACCCGTTCG AGCCACCAAG 
AAACGAAGTT CCAAAAAGGC TTTACCGACC CTCATCGCCG ACTACGAAGA GCCCAGCTCC 
GACGGTCCTC ACGGTCCTCT CCACGCCAAC GGCTATGAGC GCGATAACTT TGTCGTATCC 
GATAATGTTG AACCCGAAGA GGAAGAAGAT GCCTTCGAAC CTGTCCGCCC CTCGCGGCGC 
GGCCCATCTT CTCGCGCTAC CCGCCCTCAA CACCGCCAGA CCACCCTTTA TGACACCCTC 
TCCCACACCC AACAATCCCA AACCGTCTCC CAACACCTCG CCACTTTGGG TCCGCCCATC 
GACGCCCGCA CCATGCATAA CCCCCGCTAC GCCCAGCTTG ACGAGGTCCA CCAGGATATT 
GTCGATGCCT TTGTTGAAGA AGTCAAGGTC TTCGAGGAGG ACTTTCGCAA CAGGAACCAC 
ATGCGCAAAC CCATCTTTAC CGAGACGCAG TACCGTGAGA TGGCAATCCG GTGGACGCGG 
TCGTTAGACG CGATGCGCGC GATCCCGGAT ATCAACCAGG ATAAAGTAGA TCGGTATGGT 
GCCAAATTCA TCCCACTTGT GGAGCGGTTC TGGGGGAATT ATCAGGAGAT GATGGGGGGA 
GGGTATGATA ATCCTGCTGT GGCTGGCGAT GAGGATGATG ATGAGGGCCC CAGGAGGACA 
GGAAATGGAA AAGGGGGGAA TAAGAAGGGA GGAGGAGGAG GAGGAGGAAA TGAAGTAGTG 
GATTTGATTA GTAGTGATGA GGATGAACCT CCGGCTCGTG CACCATCGCG GAATGCGGGG 
CGAGGAAAGG CACAGTCGAC ACGTGGGGGA CAAATCCAAG ATAAAGGCCG AGCAGTCAAC 
CGCCGCGGAG AACCCATCGC CGAAGAAGAC GAAGAAGACT ACGGGCTAAG CGACCCCGAT 
ATCGACGCCA TCGATCCAGA CGCCATCACC GCCTCCGACA ACTCCGACGA AGAAGATGAT 
GATGATGATG ACGAAGACCT CGAATCCTCC CGCTACTTCT CCGGCTCAAC AGGCCCGCCC 
GTCTCCAAAG CCGTGCAGGA TGCTCGACTC CGTGAACAAC TTTCCATGTA CGCCTCCGGC 
GGCAGCTCTT CGAAAGGTAG CTACGGCTCA GGGCGCGCAT CAGGAGGATC TTCGTCGAGA 
GCGTCGGGAT CAGGATGGAG AGGTGGAGGA GCAGGTGGGA AGAAATACTA CAGGAAGAAG 
AGGGCTGGTT CTTCGGCTGC TGGTGGTGGT GGTGCAGGAG GAGGGGGAGT TACAAAACGG 
AAGGCGAGTG GGAGTGGCGC GAAGACGGCG AGGAAGAGGG GTGCATCTAC TGCGCCGAAG 
ACAACGACGA GAGGGGGAGG ATCTGGAGCT GGGTCTAGAG GAGGCGGTGC TGGTGGTGCT 
GGTGGTGCTG GTGCTGGTGC TGGTGCTGGA GGAGGGAAAA GGGGTGGTGG AGGTGGAGGA 
GGAATGGGAG GGATAAGTGT TATGCCTCAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_964030.3
CDS	1..6993
Translation	MAEREREGTSSSSSSSTSRGTSRGTTPLSLSPPPQEQEQEQQQQQQQQQQEQQNTSDTRSNQLSSSLRTFTTSATSATSSSVQQNTSNHQVLLPLPSTSSSPSTSTSFSSSLSSWVPHSLKVLYHFTAGSVTAAASTSSSSSNSGGDTVLNPNASGGVLGRLLTSSTAQSTLMTRNNWGEQVSWLKRSGNSIVVPQGPVYPSAAASIQTTTPTSTTTTTTPSAIEDGYSSTSSFPRDNNLFPLYSTPAVTNPQVSGSESLGGAGRGTGRGRGGGRVGGGGPVQQQQQPPPLGPPPPLPPPPPPPPPQQPTQQRTQQPTPNPTSTSTSTSTLATSRYTNTSSTNDARPATRQQIAPEVGASTHQDSVGLGEGGGGGMAKLSVKNNLPRPHLVSLSSSTTGSGSGSASRSASAKHGSAGSSTFDHEQHQQHQQQQQQKRQRSQSEARQQQQQQQQQQQQQQQQQQAQHHAHSTYAQRPQPTPQQRPPQNLLTPASTTGASVGPLQRAYSVSLAARQSPSTNLVRPKTDSPAPHTLHLKNKKNLRHPAPTPDSPIVDDDIFSDAVDLTEELDHDHDLNGKDKDNTDNDNTVASSSLIGFGDDKLLWREDFAERAEPEHERGGSRPRQVKKRKISNDYIMKDEDVSLFDDDGEEDEFMDINELVQGDRESTPKPKATSRSVSTRLPPTVSLQRGRSPKRKEASVEKRTTENQQQADREDEPSFMSSPDVDNSRKRKSSGSPTGLTTPRPQQKQTEEVPGTTTAKKPRRSEVMDSEDEAFTPLSAGSLPGSAEFFRSGGTTTRELGLDEDTVMDTPSRPPVESTLPTLESVESRPPPLPPMDLPSQRKPLEPLNTPRNQLLESVERPTQQPSVGPSFAQSSTLAESSLPPSMPPPSEDPLNTRENSNLEEFDYKLYKPLLDLFVNAPAILERELSAVNDELQENMIKLRDCLRLPREERDRAREEVKKEKEMLKRRDIALRALQDEHKLYVKKRKEHNLINEEIVRAYAEEDDEYEDQLMAQLDKLDDEVEAIVKSLTRLIVAAGITEKSFDLKKEEEEEEEKPIIIATPTPSTRTEAPVLPTTEYHNSQQVILQTQHPAAQQVSHRVPPPPTPSFQTARQTPVSYQSRPTNNSFPDISAEEAMMFDKEDPFMEQQHAPASAPFQATLPQRNSPFKTAPFKPVHGHDYFDDEDDDADLLAAVDSAETYTSTAATTTTNNNNHLRSQSVMSTSTATTIKPRKRNENANAKKAKSVHAKLSMPPEKMKYAWSNDVRKALKDRFRMSGFRQNQLEAINATLGGKDAFVLMPTGGGKSLCYQLPAVVRSGKTRGITVVISPLLSLMLDQVNHLANLMIQAYAFNGDMNSEMRRMVFQKLDAEHPEHELQLLYVTPEMVSKNQTFVNKMMDLYRRKKLARIVIDEAHCVSQWGHDFRPDYKAIGEFRKRFPGVPVMALTATATQNVILDVKHNLAMEDCQTFSQSFNRPNLYYEVRMKEQNLIARIAELIKEKYDGQTGIIYTLSRKSAENIAKNLQEKHRIKAKHYHASITTDEKISVQHEWQTGRVKVVVATIAFGMGIDKPDVRFVIHQHIPKSLEGYYQETGRAGRDGKPSDCYLYFAYGDIQSLRRMIADGEGDYAQKERQLQMLNRVVSYCESQHTCRREEVLRYFGEEFDYRKCRDGCDNCRNGRISKSTEMRDFTEIAFAAIEVVKSQQPITLGKLCDILMGKRKNEHGGVCHFGIAKGSTQRELQRIVLQLNFHKALGEDNIMNGAGMPITYYITGPEAGAYLYNGKRLMLPVPSNKSVEPPSRSKQRSRRVDEDMDEQELPTLQRPPTSTNVSSPVRATKKRSSKKALPTLIADYEEPSSDGPHGPLHANGYERDNFVVSDNVEPEEEEDAFEPVRPSRRGPSSRATRPQHRQTTLYDTLSHTQQSQTVSQHLATLGPPIDARTMHNPRYAQLDEVHQDIVDAFVEEVKVFEEDFRNRNHMRKPIFTETQYREMAIRWTRSLDAMRAIPDINQDKVDRYGAKFIPLVERFWGNYQEMMGGGYDNPAVAGDEDDDEGPRRTGNGKGGNKKGGGGGGGNEVVDLISSDEDEPPARAPSRNAGRGKAQSTRGGQIQDKGRAVNRRGEPIAEEDEEDYGLSDPDIDAIDPDAITASDNSDEEDDDDDDEDLESSRYFSGSTGPPVSKAVQDARLREQLSMYASGGSSSKGSYGSGRASGGSSSRASGSGWRGGGAGGKKYYRKKRAGSSAAGGGGAGGGGVTKRKASGSGAKTARKRGASTAPKTTTRGGGSGAGSRGGGAGGAGGAGAGAGAGGGKRGGGGGGGMGGISVMPH

Target ORF information:

RefSeq Version	XM_958937.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A RecQ family helicase (qde-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_958937.3

ORF Insert Sequence:

ATGGCGGAAC GGGAACGGGA AGGAACGAGT TCGAGTAGTA GTAGTAGTAC TAGTAGAGGT 
ACTAGTAGAG GTACTACCCC ACTGTCTCTG TCGCCGCCGC CGCAGGAGCA GGAGCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA GAGCAGCAGA ACACGTCTGA CACGCGATCC 
AATCAGTTGT CTTCGTCGTT GAGGACTTTT ACTACTTCTG CGACTTCTGC GACTTCCTCG 
TCGGTGCAGC AGAATACCTC TAACCACCAG GTGCTGCTGC CGCTGCCTTC CACGTCTTCG 
TCTCCTTCCA CGTCTACGTC TTTCAGTTCG TCGCTGTCGT CGTGGGTGCC TCATTCCCTA 
AAAGTTCTTT ACCATTTCAC TGCCGGCTCA GTTACTGCTG CTGCCTCTAC TAGTAGTAGT 
AGCAGTAATA GTGGTGGTGA TACAGTGCTA AATCCCAATG CGTCCGGCGG CGTGCTCGGC 
CGGTTGCTCA CCAGTTCGAC AGCGCAATCC ACGCTCATGA CGCGCAACAA TTGGGGGGAG 
CAAGTTTCTT GGTTGAAGAG ATCGGGAAAT TCCATTGTCG TTCCACAGGG GCCCGTGTAT 
CCTTCTGCTG CTGCTTCGAT ACAGACTACG ACACCAACAT CAACAACAAC AACAACAACA 
CCAAGCGCGA TCGAAGACGG TTATTCCTCG ACGAGCAGTT TTCCACGAGA TAACAATTTA 
TTCCCCTTGT ATTCCACTCC GGCTGTCACC AATCCACAAG TCAGTGGAAG TGAAAGTCTT 
GGAGGAGCAG GAAGAGGAAC AGGAAGAGGA AGAGGAGGAG GAAGAGTGGG AGGAGGAGGA 
CCAGTCCAGC AGCAACAACA ACCACCACCA CTGGGACCAC CACCACCTCT ACCACCACCA 
CCACCACCAC CACCACCACA ACAACCAACT CAACAACGAA CTCAACAACC AACCCCAAAC 
CCAACCTCGA CCTCAACCTC AACCTCAACC CTTGCGACCT CGAGATACAC AAACACATCC 
TCGACAAATG ACGCCCGACC CGCTACACGC CAACAGATTG CCCCCGAGGT AGGAGCATCG 
ACGCATCAGG ATTCAGTTGG ACTTGGAGAA GGAGGAGGAG GAGGAATGGC GAAGCTCTCA 
GTCAAGAACA ACCTGCCACG GCCGCACTTG GTCTCCTTGT CGTCGTCAAC GACAGGCTCT 
GGGTCTGGTT CTGCGTCTAG GTCAGCTTCT GCTAAGCACG GAAGTGCCGG TTCCAGTACC 
TTTGATCATG AACAACATCA ACAACATCAA CAACAACAAC AACAAAAGCG CCAGCGGTCG 
CAATCAGAAG CACGACAACA GCAGCAGCAA CAGCAACAGC AACAGCAACA GCAACAACAA 
CAACAACAAG CACAGCACCA TGCACATTCT ACATATGCAC AAAGACCCCA ACCCACCCCC 
CAACAACGAC CACCCCAAAA CCTACTGACA CCTGCTTCAA CCACTGGTGC CAGCGTCGGC 
CCGCTCCAAC GCGCATACTC GGTTTCATTA GCTGCGAGAC AGTCCCCCTC GACAAACTTG 
GTCCGTCCAA AGACCGACTC GCCAGCTCCC CACACTTTAC ACCTCAAGAA CAAGAAGAAC 
CTCCGTCACC CCGCCCCCAC GCCCGACAGT CCGATCGTAG ACGACGATAT TTTCTCCGAC 
GCCGTCGATC TTACCGAAGA ACTCGATCAT GACCATGATC TCAACGGCAA AGACAAAGAC 
AACACCGACA ACGACAACAC AGTCGCTTCC AGTTCGCTAA TAGGGTTCGG CGATGACAAG 
TTACTGTGGC GAGAGGACTT TGCTGAGCGT GCAGAGCCCG AACATGAAAG AGGTGGGAGC 
AGGCCTCGCC AGGTCAAGAA ACGGAAGATA TCGAATGACT ACATTATGAA GGATGAGGAT 
GTCTCGCTTT TTGATGATGA TGGCGAGGAG GACGAGTTTA TGGATATCAA TGAGCTAGTT 
CAGGGGGATC GGGAAAGTAC TCCGAAGCCA AAGGCTACAT CGAGGTCTGT CTCGACGAGG 
CTGCCGCCTA CAGTATCGCT GCAACGGGGT CGGTCTCCTA AGAGGAAGGA GGCTTCAGTT 
GAAAAGCGCA CAACGGAAAA CCAGCAACAG GCTGACAGAG AAGACGAACC GTCGTTTATG 
TCAAGTCCAG ATGTCGACAA CTCCCGCAAG CGAAAGTCTT CTGGATCGCC CACAGGTTTA 
ACGACGCCAA GACCCCAGCA GAAGCAAACG GAAGAGGTCC CAGGTACGAC CACCGCCAAG 
AAGCCACGGC GCAGTGAAGT GATGGACTCG GAGGACGAGG CATTCACTCC TCTTTCTGCT 
GGGTCGCTGC CTGGGAGTGC GGAGTTCTTC AGAAGCGGTG GGACCACCAC ACGGGAATTG 
GGTTTGGACG AAGACACGGT TATGGACACG CCTAGTAGGC CACCGGTCGA GTCCACTTTG 
CCAACTCTCG AGTCTGTGGA AAGTCGACCA CCCCCCCTGC CGCCCATGGA TCTACCATCA 
CAGCGAAAAC CGCTAGAGCC GTTGAACACT CCGCGCAACC AGCTGCTTGA GTCGGTCGAA 
AGGCCAACAC AGCAGCCGTC GGTGGGGCCG AGTTTTGCAC AGAGTAGCAC ACTCGCCGAA 
AGCTCCCTGC CGCCGTCAAT GCCGCCGCCA AGTGAAGACC CCCTCAACAC CAGGGAGAAC 
AGCAACCTTG AGGAGTTCGA CTACAAGCTT TACAAACCCC TGCTAGATCT TTTCGTCAAC 
GCACCCGCAA TCTTGGAAAG AGAACTGAGC GCCGTTAATG ACGAGCTTCA GGAGAACATG 
ATCAAGCTGC GGGACTGTCT GCGCCTGCCC AGGGAAGAAA GAGACAGGGC ACGCGAAGAG 
GTGAAGAAGG AAAAGGAAAT GCTCAAGCGA CGGGACATTG CGCTCAGAGC CCTCCAGGAC 
GAACACAAGT TGTACGTCAA GAAACGCAAA GAGCATAATT TGATCAACGA GGAAATCGTT 
CGCGCTTATG CTGAAGAAGA CGATGAGTAC GAGGATCAGT TAATGGCGCA GCTGGACAAG 
TTGGATGATG AGGTTGAGGC TATCGTAAAG AGTCTGACAA GGCTTATTGT GGCGGCGGGG 
ATCACGGAGA AGAGCTTTGA CCTAAAGAAG GAGGAGGAAG AGGAGGAGGA GAAGCCGATC 
ATCATAGCGA CTCCGACACC TTCGACGAGG ACCGAGGCCC CGGTTCTGCC GACGACCGAG 
TATCATAATT CCCAGCAGGT CATATTGCAG ACTCAACATC CTGCTGCGCA GCAGGTTTCT 
CACCGGGTGC CACCACCTCC GACACCGAGT TTTCAAACGG CGCGCCAGAC TCCGGTGTCA 
TATCAGAGCA GACCGACCAA CAACTCCTTT CCTGATATCT CGGCGGAAGA AGCCATGATG 
TTCGATAAAG AAGACCCCTT CATGGAACAA CAGCACGCCC CGGCCTCTGC TCCCTTCCAG 
GCCACCCTTC CCCAGCGCAA CAGCCCTTTC AAAACCGCCC CGTTCAAGCC AGTCCACGGC 
CACGATTACT TTGACGATGA AGACGACGAT GCCGACCTCC TGGCAGCAGT AGACAGCGCC 
GAGACGTATA CTTCTACGGC CGCCACCACC ACCACCAACA ACAACAATCA CTTACGATCA 
CAATCGGTGA TGTCAACATC CACGGCGACC ACGATCAAAC CGAGGAAACG CAACGAAAAT 
GCCAATGCCA AGAAGGCCAA GTCCGTACAT GCAAAGTTAT CGATGCCGCC CGAAAAGATG 
AAGTATGCGT GGTCGAATGA TGTGAGGAAG GCTCTCAAGG ATAGGTTTCG GATGTCGGGG 
TTCAGACAGA ATCAGTTGGA GGCTATTAAT GCTACTTTGG GTGGTAAGGA TGCCTTTGTG 
TTGATGCCGA CTGGTGGTGG AAAGTCTCTG TGCTATCAGT TGCCGGCTGT AGTCAGGAGC 
GGCAAGACGC GTGGTATCAC AGTCGTCATC TCCCCTCTGC TAAGTCTGAT GCTGGATCAA 
GTCAACCATT TGGCAAACCT GATGATCCAA GCTTACGCTT TCAACGGAGA CATGAACTCA 
GAAATGCGCC GAATGGTGTT TCAGAAGCTT GATGCTGAGC ATCCTGAGCA TGAGCTCCAA 
CTGCTCTATG TCACCCCGGA GATGGTGAGC AAGAACCAGA CATTCGTCAA CAAGATGATG 
GACCTCTACC GGAGGAAAAA GCTGGCTAGA ATTGTTATCG ACGAGGCTCA CTGCGTCAGT 
CAATGGGGCC ATGACTTCCG ACCCGATTAC AAAGCTATAG GAGAGTTTCG TAAGAGGTTT 
CCCGGAGTTC CGGTCATGGC TTTGACAGCG ACGGCAACAC AGAACGTCAT CTTGGATGTC 
AAGCATAACC TGGCAATGGA GGACTGCCAG ACTTTCTCCC AGAGCTTTAA TCGGCCGAAC 
CTCTACTATG AGGTCAGGAT GAAGGAGCAG AATCTGATTG CCCGCATCGC AGAGTTGATC 
AAGGAGAAGT ATGACGGCCA GACGGGTATC ATCTACACAT TATCAAGAAA GAGTGCCGAG 
AACATCGCCA AAAATCTCCA GGAAAAACAC CGCATCAAAG CAAAGCACTA CCATGCCTCC 
ATCACCACCG ACGAAAAGAT CAGCGTTCAA CATGAATGGC AAACCGGCCG AGTCAAAGTC 
GTGGTAGCCA CCATTGCCTT CGGCATGGGC ATCGACAAGC CTGACGTCCG CTTTGTTATC 
CACCAGCACA TCCCCAAGTC GCTCGAAGGT TACTATCAAG AAACCGGCCG CGCCGGACGT 
GACGGCAAGC CATCGGACTG CTACTTGTAC TTTGCCTATG GCGACATTCA ATCCCTACGT 
CGTATGATCG CCGACGGCGA AGGTGACTAC GCGCAAAAGG AGCGTCAGCT ACAAATGCTC 
AACCGTGTGG TCAGCTATTG CGAGTCGCAG CACACGTGCC GGCGCGAAGA AGTGCTCCGC 
TACTTTGGCG AGGAGTTTGA CTACCGGAAG TGTAGAGACG GATGCGATAA CTGCCGGAAC 
GGACGCATCT CGAAGTCGAC GGAGATGAGG GATTTTACGG AAATCGCCTT CGCCGCGATC 
GAGGTGGTGA AGAGCCAGCA GCCCATCACG CTGGGCAAGC TGTGCGACAT CCTGATGGGC 
AAGAGAAAGA ACGAGCACGG TGGCGTGTGT CACTTTGGTA TCGCCAAGGG GAGCACGCAG 
AGGGAGCTGC AGAGGATCGT GCTGCAGCTG AATTTCCACA AGGCGCTGGG CGAGGACAAT 
ATCATGAATG GGGCGGGGAT GCCTATTACC TACTATATTA CCGGCCCTGA AGCTGGTGCT 
TACCTCTACA ATGGCAAGCG GTTGATGCTG CCAGTTCCCT CAAACAAGTC CGTCGAACCC 
CCGTCTCGGT CTAAGCAGCG GAGCCGTCGA GTCGATGAGG ATATGGATGA GCAAGAACTT 
CCCACCCTGC AACGACCGCC AACATCAACA AATGTCTCTT CACCCGTTCG AGCCACCAAG 
AAACGAAGTT CCAAAAAGGC TTTACCGACC CTCATCGCCG ACTACGAAGA GCCCAGCTCC 
GACGGTCCTC ACGGTCCTCT CCACGCCAAC GGCTATGAGC GCGATAACTT TGTCGTATCC 
GATAATGTTG AACCCGAAGA GGAAGAAGAT GCCTTCGAAC CTGTCCGCCC CTCGCGGCGC 
GGCCCATCTT CTCGCGCTAC CCGCCCTCAA CACCGCCAGA CCACCCTTTA TGACACCCTC 
TCCCACACCC AACAATCCCA AACCGTCTCC CAACACCTCG CCACTTTGGG TCCGCCCATC 
GACGCCCGCA CCATGCATAA CCCCCGCTAC GCCCAGCTTG ACGAGGTCCA CCAGGATATT 
GTCGATGCCT TTGTTGAAGA AGTCAAGGTC TTCGAGGAGG ACTTTCGCAA CAGGAACCAC 
ATGCGCAAAC CCATCTTTAC CGAGACGCAG TACCGTGAGA TGGCAATCCG GTGGACGCGG 
TCGTTAGACG CGATGCGCGC GATCCCGGAT ATCAACCAGG ATAAAGTAGA TCGGTATGGT 
GCCAAATTCA TCCCACTTGT GGAGCGGTTC TGGGGGAATT ATCAGGAGAT GATGGGGGGA 
GGGTATGATA ATCCTGCTGT GGCTGGCGAT GAGGATGATG ATGAGGGCCC CAGGAGGACA 
GGAAATGGAA AAGGGGGGAA TAAGAAGGGA GGAGGAGGAG GAGGAGGAAA TGAAGTAGTG 
GATTTGATTA GTAGTGATGA GGATGAACCT CCGGCTCGTG CACCATCGCG GAATGCGGGG 
CGAGGAAAGG CACAGTCGAC ACGTGGGGGA CAAATCCAAG ATAAAGGCCG AGCAGTCAAC 
CGCCGCGGAG AACCCATCGC CGAAGAAGAC GAAGAAGACT ACGGGCTAAG CGACCCCGAT 
ATCGACGCCA TCGATCCAGA CGCCATCACC GCCTCCGACA ACTCCGACGA AGAAGATGAT 
GATGATGATG ACGAAGACCT CGAATCCTCC CGCTACTTCT CCGGCTCAAC AGGCCCGCCC 
GTCTCCAAAG CCGTGCAGGA TGCTCGACTC CGTGAACAAC TTTCCATGTA CGCCTCCGGC 
GGCAGCTCTT CGAAAGGTAG CTACGGCTCA GGGCGCGCAT CAGGAGGATC TTCGTCGAGA 
GCGTCGGGAT CAGGATGGAG AGGTGGAGGA GCAGGTGGGA AGAAATACTA CAGGAAGAAG 
AGGGCTGGTT CTTCGGCTGC TGGTGGTGGT GGTGCAGGAG GAGGGGGAGT TACAAAACGG 
AAGGCGAGTG GGAGTGGCGC GAAGACGGCG AGGAAGAGGG GTGCATCTAC TGCGCCGAAG 
ACAACGACGA GAGGGGGAGG ATCTGGAGCT GGGTCTAGAG GAGGCGGTGC TGGTGGTGCT 
GGTGGTGCTG GTGCTGGTGC TGGTGCTGGA GGAGGGAAAA GGGGTGGTGG AGGTGGAGGA 
GGAATGGGAG GGATAAGTGT TATGCCTCAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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qde-3 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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