chs-4 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	chs-4
Entrez Gene ID	3880177
Full Name	chitin synthase-4
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011394972.1	XP_011393274.1	chitin synthase 4, variant
XM_011394973.1	XP_011393275.1	chitin synthase 4

Pathways from BioSystems

Homologies

Neurospora crassa	NCU09324	XP_964028.2
Saccharomyces cerevisiae (baker's yeast)	CHS3	NP_009579.1
Kluyveromyces lactis	KLLA0D03498g	XP_453222.1
Magnaporthe oryzae (rice blast fungus)	MGG_09962	XP_003710073.1
Eremothecium gossypii	AGOS_AEL189W	NP_984672.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chs-4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chs-4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf02489	XM_011394973.1 Latest version!	Neurospora crassa OR74A chitin synthase 4 (chs-4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ONf02489	XM_011394972.1 Latest version!	Neurospora crassa OR74A chitin synthase 4, variant (chs-4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chs-4 ( XM_011394972.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393274.1 Neurospora crassa OR74A chitin synthase 4, variant (chs-4), mRNA.

Price & Availability↑

CloneID	ONf02489
Clone ID Related Accession (Same CDS sequence)	XM_011394972.1 , XM_011394973.1
Accession Version	XM_011394972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	chitin synthase 4, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

ATGTCTCTTC CTCGGAGGCC GGGGCCGTCG CCTCCGCACG AGGAGCCACG CCGCTACCGG 
CAAAGCGGTT CGCGCCGCAG TCGACCACCA CCCGCCGATG TTGAGACCGG ATATGCTCCC 
ATGGCAGGAG AAAGACCAAG CCAGCAGCAA CGCGTACCAT CGATATCTTC ATTTCCGGAA 
ACCCTCCCGT CCCCCAACCC CAATGTCGAA CGAGACCCTT TGGCGCCCAC CCCCGAGCCC 
GCACATCCAT CCGGCATACC TCAGCGGAAG AGGAGTTTGA TTCGACCGGA GCGCAACAGG 
ATTGGCAAAG ACCACCCCAA CTATCACTAC AGAAAACATG CGGCCAACAT GAACACCCTA 
CCGTCCTCAA CCGGACACGA CCCGATATAC GAGGATCTGG AGGGAGCAAC GGACGATGTA 
TCGGGAACTG GCTCGAGGAA CGACGACGAT GTTTCCGAGG AGTCGCCCCC AAGAAGAAAG 
CACTCGACGA AAATGCAGGT CATCGAAACT GAAAAGTCCG GTGATGAAAG ACGACGTCGG 
CGGAAATCGG ATACCACAAA ACATGGTAAA ATTGTCAAGG CCTCCAAGGG AAAGCGCGAA 
AAGTCCGGAG GACTCCCTAC CCCGAGCTTT TGGAACATTT ACTGCGGATT CGTCACCTTC 
TGGTGCCCTG GCTTTGTCCT CAAGTGCTTC GGCATGCCCG AGATGGCTCA ACAGAGGGCG 
TGGAGAGAGA AGATGGGTCT GATCAGCATT ATTCTGCTCA TCATGGGCTT TGTTGGCTTC 
ATCACTTTCG GCTTCACCCA GGTGGTCTGC GGTAAACCTC CGCTCCGTTT GCGCATCAAT 
GAGGTCGGGT CAGGATACAT GATCTTCCAC GGATCTGCAT ATGACCTCAC CAAATCTCAC 
CATCCTCCGG CCGAGGGTAT CCCGCGCCGT CCGGACGGTT TGGGTGCAAA CGTGATCTAT 
GACTTGCCTC AGCATTACGG TGGACAGGAC GGCAGTTTCT TGTTCCAGAA CGTCAACGGC 
AAGTGCAAGG GTCTCATCAC CAAGCAAGAG AACTCCGATG TGCCAAGCGA TAAGAGCGGC 
AATCTGGCGT GGTACTTCCC TTGTAACACG TTCAACCAAG ATGGTTCATC CAAGCCCAAT 
ACCACGATAC CTTATTACCT GGGCTATGCC TGTCACACGA CCGCTAACGC CAGGGACTCT 
TTCTATCTCG GCCTCAAGAG CTCCGCCGAT GTCTACTTTA CGTGGGATGA CATCAAGAAC 
AGCTCTCGAA ACCTTGTTGT GTACTCTGGC CATGTTCTCG ACCTTGATCT GCTTCACTGG 
TTCAACGACA CTCAAGTCAC GTATCCCGCC CGCTTCAAGG AGCTGAGGGA TAAGAATACT 
GCTGGAAACC AGGCTATTCG TGGACGCGAT ATTACCCATG CTTTCCAGTC GAGCAAAGAC 
AAGCAGATTG CCGAATGCTT TGAGGAGATC ATCAAGGTCG GTTCGGTGGA TACCGAGACA 
GTTGGCTGCA TTGCGTCAAA GGTCGTTCTC TATGTGTCCC TCGTCCTTAT CCTCGCGGTC 
GTCCTCGCCA GATTCGTCTT GGCTCTAATC TTCCAATGGT TCATCAGCAA GACGTATGCG 
GCTGCTAAGA CCTCCCAAAC GTCTGACCAG CGAAAGCGAA ACCGTCAGAT TGAAGACTGG 
ACTGAAGACA TTTACAGAGC TCCCCCAAGG CTACCAGGGG AGGTCGGTAG CAGTGTCGCT 
GGAAGCTCCG ATCGTCAAAG CAAGCGCAGC AGTGCTTTCT TGCCCACACA CTCTCGTTTC 
TCCACCGTCT ACGGCAATGA GCGCGGCAAT AGGAAGCCTG GACTTCCTAC AACCATGGCC 
AGCCAGAATG CCGCTGGTCA ACTACTCCAT CCTGGAACCA TATACGGCCA AGGAAATGAG 
AGTCGGTCAA GCTTCCTCAA ATCTGATGCT TACGGATCAA GCAGCAGTCC AGCAGACGGC 
CCAGGTCCTG CCGGATTTAT TCATGAAGCT GTCGTTCCTC AGCCGCCTTC CGACTGGATG 
CCGTTTGGTT TCCCCCTCGC TCACACCATC TGCCTTGTCA CAGCCTACTC CGAGGGTGAG 
ATGGGCGTTC GTACCACTCT GGACTCGATT GCAATGACGG ACTACCCCAA CAGCCACAAG 
GTTATCCTCG TTATTTGCGA TGGTATTATC AAGGGCCACG GAGAGGAACA CTCAACCCCC 
GATATCATTC TCGGCATGAT GAAAGACCAC ACGATTCACC CCGATGACGT TGAGCCATTC 
TCTTATGTTG CGGTTGCAAC TGGTTCTAAG CGCCACAACA TGGCCAAGGT CTACACCGGC 
TTCTACGATT ATGGCACTAA CTCTGCCATT CCTCTGGAGA AGCAGCAGCG TGTTCCTATG 
ATGATGGTTG TGAAGTGCGG CACTCCTGCT GAAGCCTCCA AGTCGAAGCC TGGAAACCGT 
GGCAAGCGTG ACAGTCAGAT CATCCTTATG TCTTTTCTCC AGAAGGTTAT GTTCGACGAG 
CGTATGACGG AGCTAGAATA CGAGATGTTC AATGGCTTGT GGAAGATCAC GGGCATTTCT 
CCTGACTTCT ATGAGATCGT GCTCATGGTT GACGCTGATA CCAAGGTCTT CCCCGACAGT 
CTCACGCATA TGATCTCGGC CATGGTCAAG GATCCTGAAA TTATGGGTCT TTGTGGCGAG 
ACCAAGATCG CCAACAAGCG AGCAAGCTGG GTTTCCGCCA TCCAGGTATT CGAGTATTTC 
GTCTCTCATC ATCTTGCCAA GGCTTTCGAG TCCGTCTTTG GCGGTGTCAC CTGTCTTCCC 
GGATGTTTCT GCATGTACCG CATCAAGGCA CCCAAGGGTG CCCAGAACTA CTGGGTCCCT 
ATCTTGGCCA ACCCGGACGT TGTCGAACAC TACTCCGAGA ACGTTGTCGA TACGCTGCAC 
AAGAAGAACC TCTTGCTCCT TGGTGAGGAT CGTTATCTCA GTACGCTCAT GTTGCGCACG 
TTCCCCAAGC GTAAACAAGT ATTTGTGCCT CAGGCCGTTT GCAAGACGAC TGTCCCCGAT 
GAATTCATGG TGTTGCTTTC GCAGCGCCGC AGATGGATCA ACTCGACCAT TCACAATCTG 
ATGGAGCTCG TTCTTGTGCG CGATCTTTGC GGTACCTTCT GTTTCAGTAT GCAGTTCATT 
GTCGGTATCG AGCTGATTGG TACCCTTGTG TTGCCTGCCG CCATTGCCTT TACTTTCTAT 
GTTGTCATCA TCTCAATCAT CAACTCCCCG CCACAAATCA TTCCTCTTGT TCTCCTTGGT 
CTCATCCTCG GTCTCCCAGC CATCCTCGTC GTCGTCACAG CTCACAGCTG GTCCTACATC 
ATCTGGATGT TTATCTACTT GCTCTCCCTG CCCGTGTGGA ACTTTGTTCT GCCTACCTAC 
GCTTTCTGGA AGTTTGACGA TTTCTCATGG GGCGACACGC GCAAGACGGC CGGCGAGAAA 
TCAAGCAAGG GTGGCCACGG AGAAGCCGAG GGAGAGTTTG ATAGCAGCAT GATTACCATG 
AAGCGCTGGG CCGAGTTTGA GCGCGACAGG CGCGTCCGCA GCACGTACTG GGCCGGCTCG 
AGGGACAATG TCATATCCGG GGTAGGGGGT AGTAATGGGT GGGGAAGCAG CCAGCCGAGA 
GGCCATGAGC AGGGGAGACA CTTTGATGAC TACTTTTCGG ACGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393274.1
CDS	334..4041
Translation	MSLPRRPGPSPPHEEPRRYRQSGSRRSRPPPADVETGYAPMAGERPSQQQRVPSISSFPETLPSPNPNVERDPLAPTPEPAHPSGIPQRKRSLIRPERNRIGKDHPNYHYRKHAANMNTLPSSTGHDPIYEDLEGATDDVSGTGSRNDDDVSEESPPRRKHSTKMQVIETEKSGDERRRRRKSDTTKHGKIVKASKGKREKSGGLPTPSFWNIYCGFVTFWCPGFVLKCFGMPEMAQQRAWREKMGLISIILLIMGFVGFITFGFTQVVCGKPPLRLRINEVGSGYMIFHGSAYDLTKSHHPPAEGIPRRPDGLGANVIYDLPQHYGGQDGSFLFQNVNGKCKGLITKQENSDVPSDKSGNLAWYFPCNTFNQDGSSKPNTTIPYYLGYACHTTANARDSFYLGLKSSADVYFTWDDIKNSSRNLVVYSGHVLDLDLLHWFNDTQVTYPARFKELRDKNTAGNQAIRGRDITHAFQSSKDKQIAECFEEIIKVGSVDTETVGCIASKVVLYVSLVLILAVVLARFVLALIFQWFISKTYAAAKTSQTSDQRKRNRQIEDWTEDIYRAPPRLPGEVGSSVAGSSDRQSKRSSAFLPTHSRFSTVYGNERGNRKPGLPTTMASQNAAGQLLHPGTIYGQGNESRSSFLKSDAYGSSSSPADGPGPAGFIHEAVVPQPPSDWMPFGFPLAHTICLVTAYSEGEMGVRTTLDSIAMTDYPNSHKVILVICDGIIKGHGEEHSTPDIILGMMKDHTIHPDDVEPFSYVAVATGSKRHNMAKVYTGFYDYGTNSAIPLEKQQRVPMMMVVKCGTPAEASKSKPGNRGKRDSQIILMSFLQKVMFDERMTELEYEMFNGLWKITGISPDFYEIVLMVDADTKVFPDSLTHMISAMVKDPEIMGLCGETKIANKRASWVSAIQVFEYFVSHHLAKAFESVFGGVTCLPGCFCMYRIKAPKGAQNYWVPILANPDVVEHYSENVVDTLHKKNLLLLGEDRYLSTLMLRTFPKRKQVFVPQAVCKTTVPDEFMVLLSQRRRWINSTIHNLMELVLVRDLCGTFCFSMQFIVGIELIGTLVLPAAIAFTFYVVIISIINSPPQIIPLVLLGLILGLPAILVVVTAHSWSYIIWMFIYLLSLPVWNFVLPTYAFWKFDDFSWGDTRKTAGEKSSKGGHGEAEGEFDSSMITMKRWAEFERDRRVRSTYWAGSRDNVISGVGGSNGWGSSQPRGHEQGRHFDDYFSDA

Target ORF information:

RefSeq Version	XM_011394972.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A chitin synthase 4, variant (chs-4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394972.1

ORF Insert Sequence:

ATGTCTCTTC CTCGGAGGCC GGGGCCGTCG CCTCCGCACG AGGAGCCACG CCGCTACCGG 
CAAAGCGGTT CGCGCCGCAG TCGACCACCA CCCGCCGATG TTGAGACCGG ATATGCTCCC 
ATGGCAGGAG AAAGACCAAG CCAGCAGCAA CGCGTACCAT CGATATCTTC ATTTCCGGAA 
ACCCTCCCGT CCCCCAACCC CAATGTCGAA CGAGACCCTT TGGCGCCCAC CCCCGAGCCC 
GCACATCCAT CCGGCATACC TCAGCGGAAG AGGAGTTTGA TTCGACCGGA GCGCAACAGG 
ATTGGCAAAG ACCACCCCAA CTATCACTAC AGAAAACATG CGGCCAACAT GAACACCCTA 
CCGTCCTCAA CCGGACACGA CCCGATATAC GAGGATCTGG AGGGAGCAAC GGACGATGTA 
TCGGGAACTG GCTCGAGGAA CGACGACGAT GTTTCCGAGG AGTCGCCCCC AAGAAGAAAG 
CACTCGACGA AAATGCAGGT CATCGAAACT GAAAAGTCCG GTGATGAAAG ACGACGTCGG 
CGGAAATCGG ATACCACAAA ACATGGTAAA ATTGTCAAGG CCTCCAAGGG AAAGCGCGAA 
AAGTCCGGAG GACTCCCTAC CCCGAGCTTT TGGAACATTT ACTGCGGATT CGTCACCTTC 
TGGTGCCCTG GCTTTGTCCT CAAGTGCTTC GGCATGCCCG AGATGGCTCA ACAGAGGGCG 
TGGAGAGAGA AGATGGGTCT GATCAGCATT ATTCTGCTCA TCATGGGCTT TGTTGGCTTC 
ATCACTTTCG GCTTCACCCA GGTGGTCTGC GGTAAACCTC CGCTCCGTTT GCGCATCAAT 
GAGGTCGGGT CAGGATACAT GATCTTCCAC GGATCTGCAT ATGACCTCAC CAAATCTCAC 
CATCCTCCGG CCGAGGGTAT CCCGCGCCGT CCGGACGGTT TGGGTGCAAA CGTGATCTAT 
GACTTGCCTC AGCATTACGG TGGACAGGAC GGCAGTTTCT TGTTCCAGAA CGTCAACGGC 
AAGTGCAAGG GTCTCATCAC CAAGCAAGAG AACTCCGATG TGCCAAGCGA TAAGAGCGGC 
AATCTGGCGT GGTACTTCCC TTGTAACACG TTCAACCAAG ATGGTTCATC CAAGCCCAAT 
ACCACGATAC CTTATTACCT GGGCTATGCC TGTCACACGA CCGCTAACGC CAGGGACTCT 
TTCTATCTCG GCCTCAAGAG CTCCGCCGAT GTCTACTTTA CGTGGGATGA CATCAAGAAC 
AGCTCTCGAA ACCTTGTTGT GTACTCTGGC CATGTTCTCG ACCTTGATCT GCTTCACTGG 
TTCAACGACA CTCAAGTCAC GTATCCCGCC CGCTTCAAGG AGCTGAGGGA TAAGAATACT 
GCTGGAAACC AGGCTATTCG TGGACGCGAT ATTACCCATG CTTTCCAGTC GAGCAAAGAC 
AAGCAGATTG CCGAATGCTT TGAGGAGATC ATCAAGGTCG GTTCGGTGGA TACCGAGACA 
GTTGGCTGCA TTGCGTCAAA GGTCGTTCTC TATGTGTCCC TCGTCCTTAT CCTCGCGGTC 
GTCCTCGCCA GATTCGTCTT GGCTCTAATC TTCCAATGGT TCATCAGCAA GACGTATGCG 
GCTGCTAAGA CCTCCCAAAC GTCTGACCAG CGAAAGCGAA ACCGTCAGAT TGAAGACTGG 
ACTGAAGACA TTTACAGAGC TCCCCCAAGG CTACCAGGGG AGGTCGGTAG CAGTGTCGCT 
GGAAGCTCCG ATCGTCAAAG CAAGCGCAGC AGTGCTTTCT TGCCCACACA CTCTCGTTTC 
TCCACCGTCT ACGGCAATGA GCGCGGCAAT AGGAAGCCTG GACTTCCTAC AACCATGGCC 
AGCCAGAATG CCGCTGGTCA ACTACTCCAT CCTGGAACCA TATACGGCCA AGGAAATGAG 
AGTCGGTCAA GCTTCCTCAA ATCTGATGCT TACGGATCAA GCAGCAGTCC AGCAGACGGC 
CCAGGTCCTG CCGGATTTAT TCATGAAGCT GTCGTTCCTC AGCCGCCTTC CGACTGGATG 
CCGTTTGGTT TCCCCCTCGC TCACACCATC TGCCTTGTCA CAGCCTACTC CGAGGGTGAG 
ATGGGCGTTC GTACCACTCT GGACTCGATT GCAATGACGG ACTACCCCAA CAGCCACAAG 
GTTATCCTCG TTATTTGCGA TGGTATTATC AAGGGCCACG GAGAGGAACA CTCAACCCCC 
GATATCATTC TCGGCATGAT GAAAGACCAC ACGATTCACC CCGATGACGT TGAGCCATTC 
TCTTATGTTG CGGTTGCAAC TGGTTCTAAG CGCCACAACA TGGCCAAGGT CTACACCGGC 
TTCTACGATT ATGGCACTAA CTCTGCCATT CCTCTGGAGA AGCAGCAGCG TGTTCCTATG 
ATGATGGTTG TGAAGTGCGG CACTCCTGCT GAAGCCTCCA AGTCGAAGCC TGGAAACCGT 
GGCAAGCGTG ACAGTCAGAT CATCCTTATG TCTTTTCTCC AGAAGGTTAT GTTCGACGAG 
CGTATGACGG AGCTAGAATA CGAGATGTTC AATGGCTTGT GGAAGATCAC GGGCATTTCT 
CCTGACTTCT ATGAGATCGT GCTCATGGTT GACGCTGATA CCAAGGTCTT CCCCGACAGT 
CTCACGCATA TGATCTCGGC CATGGTCAAG GATCCTGAAA TTATGGGTCT TTGTGGCGAG 
ACCAAGATCG CCAACAAGCG AGCAAGCTGG GTTTCCGCCA TCCAGGTATT CGAGTATTTC 
GTCTCTCATC ATCTTGCCAA GGCTTTCGAG TCCGTCTTTG GCGGTGTCAC CTGTCTTCCC 
GGATGTTTCT GCATGTACCG CATCAAGGCA CCCAAGGGTG CCCAGAACTA CTGGGTCCCT 
ATCTTGGCCA ACCCGGACGT TGTCGAACAC TACTCCGAGA ACGTTGTCGA TACGCTGCAC 
AAGAAGAACC TCTTGCTCCT TGGTGAGGAT CGTTATCTCA GTACGCTCAT GTTGCGCACG 
TTCCCCAAGC GTAAACAAGT ATTTGTGCCT CAGGCCGTTT GCAAGACGAC TGTCCCCGAT 
GAATTCATGG TGTTGCTTTC GCAGCGCCGC AGATGGATCA ACTCGACCAT TCACAATCTG 
ATGGAGCTCG TTCTTGTGCG CGATCTTTGC GGTACCTTCT GTTTCAGTAT GCAGTTCATT 
GTCGGTATCG AGCTGATTGG TACCCTTGTG TTGCCTGCCG CCATTGCCTT TACTTTCTAT 
GTTGTCATCA TCTCAATCAT CAACTCCCCG CCACAAATCA TTCCTCTTGT TCTCCTTGGT 
CTCATCCTCG GTCTCCCAGC CATCCTCGTC GTCGTCACAG CTCACAGCTG GTCCTACATC 
ATCTGGATGT TTATCTACTT GCTCTCCCTG CCCGTGTGGA ACTTTGTTCT GCCTACCTAC 
GCTTTCTGGA AGTTTGACGA TTTCTCATGG GGCGACACGC GCAAGACGGC CGGCGAGAAA 
TCAAGCAAGG GTGGCCACGG AGAAGCCGAG GGAGAGTTTG ATAGCAGCAT GATTACCATG 
AAGCGCTGGG CCGAGTTTGA GCGCGACAGG CGCGTCCGCA GCACGTACTG GGCCGGCTCG 
AGGGACAATG TCATATCCGG GGTAGGGGGT AGTAATGGGT GGGGAAGCAG CCAGCCGAGA 
GGCCATGAGC AGGGGAGACA CTTTGATGAC TACTTTTCGG ACGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chs-4 ( XM_011394973.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393275.1 Neurospora crassa OR74A chitin synthase 4 (chs-4), mRNA.

Price & Availability↑

CloneID	ONf02489
Clone ID Related Accession (Same CDS sequence)	XM_011394972.1 , XM_011394973.1
Accession Version	XM_011394973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	chitin synthase 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

ATGTCTCTTC CTCGGAGGCC GGGGCCGTCG CCTCCGCACG AGGAGCCACG CCGCTACCGG 
CAAAGCGGTT CGCGCCGCAG TCGACCACCA CCCGCCGATG TTGAGACCGG ATATGCTCCC 
ATGGCAGGAG AAAGACCAAG CCAGCAGCAA CGCGTACCAT CGATATCTTC ATTTCCGGAA 
ACCCTCCCGT CCCCCAACCC CAATGTCGAA CGAGACCCTT TGGCGCCCAC CCCCGAGCCC 
GCACATCCAT CCGGCATACC TCAGCGGAAG AGGAGTTTGA TTCGACCGGA GCGCAACAGG 
ATTGGCAAAG ACCACCCCAA CTATCACTAC AGAAAACATG CGGCCAACAT GAACACCCTA 
CCGTCCTCAA CCGGACACGA CCCGATATAC GAGGATCTGG AGGGAGCAAC GGACGATGTA 
TCGGGAACTG GCTCGAGGAA CGACGACGAT GTTTCCGAGG AGTCGCCCCC AAGAAGAAAG 
CACTCGACGA AAATGCAGGT CATCGAAACT GAAAAGTCCG GTGATGAAAG ACGACGTCGG 
CGGAAATCGG ATACCACAAA ACATGGTAAA ATTGTCAAGG CCTCCAAGGG AAAGCGCGAA 
AAGTCCGGAG GACTCCCTAC CCCGAGCTTT TGGAACATTT ACTGCGGATT CGTCACCTTC 
TGGTGCCCTG GCTTTGTCCT CAAGTGCTTC GGCATGCCCG AGATGGCTCA ACAGAGGGCG 
TGGAGAGAGA AGATGGGTCT GATCAGCATT ATTCTGCTCA TCATGGGCTT TGTTGGCTTC 
ATCACTTTCG GCTTCACCCA GGTGGTCTGC GGTAAACCTC CGCTCCGTTT GCGCATCAAT 
GAGGTCGGGT CAGGATACAT GATCTTCCAC GGATCTGCAT ATGACCTCAC CAAATCTCAC 
CATCCTCCGG CCGAGGGTAT CCCGCGCCGT CCGGACGGTT TGGGTGCAAA CGTGATCTAT 
GACTTGCCTC AGCATTACGG TGGACAGGAC GGCAGTTTCT TGTTCCAGAA CGTCAACGGC 
AAGTGCAAGG GTCTCATCAC CAAGCAAGAG AACTCCGATG TGCCAAGCGA TAAGAGCGGC 
AATCTGGCGT GGTACTTCCC TTGTAACACG TTCAACCAAG ATGGTTCATC CAAGCCCAAT 
ACCACGATAC CTTATTACCT GGGCTATGCC TGTCACACGA CCGCTAACGC CAGGGACTCT 
TTCTATCTCG GCCTCAAGAG CTCCGCCGAT GTCTACTTTA CGTGGGATGA CATCAAGAAC 
AGCTCTCGAA ACCTTGTTGT GTACTCTGGC CATGTTCTCG ACCTTGATCT GCTTCACTGG 
TTCAACGACA CTCAAGTCAC GTATCCCGCC CGCTTCAAGG AGCTGAGGGA TAAGAATACT 
GCTGGAAACC AGGCTATTCG TGGACGCGAT ATTACCCATG CTTTCCAGTC GAGCAAAGAC 
AAGCAGATTG CCGAATGCTT TGAGGAGATC ATCAAGGTCG GTTCGGTGGA TACCGAGACA 
GTTGGCTGCA TTGCGTCAAA GGTCGTTCTC TATGTGTCCC TCGTCCTTAT CCTCGCGGTC 
GTCCTCGCCA GATTCGTCTT GGCTCTAATC TTCCAATGGT TCATCAGCAA GACGTATGCG 
GCTGCTAAGA CCTCCCAAAC GTCTGACCAG CGAAAGCGAA ACCGTCAGAT TGAAGACTGG 
ACTGAAGACA TTTACAGAGC TCCCCCAAGG CTACCAGGGG AGGTCGGTAG CAGTGTCGCT 
GGAAGCTCCG ATCGTCAAAG CAAGCGCAGC AGTGCTTTCT TGCCCACACA CTCTCGTTTC 
TCCACCGTCT ACGGCAATGA GCGCGGCAAT AGGAAGCCTG GACTTCCTAC AACCATGGCC 
AGCCAGAATG CCGCTGGTCA ACTACTCCAT CCTGGAACCA TATACGGCCA AGGAAATGAG 
AGTCGGTCAA GCTTCCTCAA ATCTGATGCT TACGGATCAA GCAGCAGTCC AGCAGACGGC 
CCAGGTCCTG CCGGATTTAT TCATGAAGCT GTCGTTCCTC AGCCGCCTTC CGACTGGATG 
CCGTTTGGTT TCCCCCTCGC TCACACCATC TGCCTTGTCA CAGCCTACTC CGAGGGTGAG 
ATGGGCGTTC GTACCACTCT GGACTCGATT GCAATGACGG ACTACCCCAA CAGCCACAAG 
GTTATCCTCG TTATTTGCGA TGGTATTATC AAGGGCCACG GAGAGGAACA CTCAACCCCC 
GATATCATTC TCGGCATGAT GAAAGACCAC ACGATTCACC CCGATGACGT TGAGCCATTC 
TCTTATGTTG CGGTTGCAAC TGGTTCTAAG CGCCACAACA TGGCCAAGGT CTACACCGGC 
TTCTACGATT ATGGCACTAA CTCTGCCATT CCTCTGGAGA AGCAGCAGCG TGTTCCTATG 
ATGATGGTTG TGAAGTGCGG CACTCCTGCT GAAGCCTCCA AGTCGAAGCC TGGAAACCGT 
GGCAAGCGTG ACAGTCAGAT CATCCTTATG TCTTTTCTCC AGAAGGTTAT GTTCGACGAG 
CGTATGACGG AGCTAGAATA CGAGATGTTC AATGGCTTGT GGAAGATCAC GGGCATTTCT 
CCTGACTTCT ATGAGATCGT GCTCATGGTT GACGCTGATA CCAAGGTCTT CCCCGACAGT 
CTCACGCATA TGATCTCGGC CATGGTCAAG GATCCTGAAA TTATGGGTCT TTGTGGCGAG 
ACCAAGATCG CCAACAAGCG AGCAAGCTGG GTTTCCGCCA TCCAGGTATT CGAGTATTTC 
GTCTCTCATC ATCTTGCCAA GGCTTTCGAG TCCGTCTTTG GCGGTGTCAC CTGTCTTCCC 
GGATGTTTCT GCATGTACCG CATCAAGGCA CCCAAGGGTG CCCAGAACTA CTGGGTCCCT 
ATCTTGGCCA ACCCGGACGT TGTCGAACAC TACTCCGAGA ACGTTGTCGA TACGCTGCAC 
AAGAAGAACC TCTTGCTCCT TGGTGAGGAT CGTTATCTCA GTACGCTCAT GTTGCGCACG 
TTCCCCAAGC GTAAACAAGT ATTTGTGCCT CAGGCCGTTT GCAAGACGAC TGTCCCCGAT 
GAATTCATGG TGTTGCTTTC GCAGCGCCGC AGATGGATCA ACTCGACCAT TCACAATCTG 
ATGGAGCTCG TTCTTGTGCG CGATCTTTGC GGTACCTTCT GTTTCAGTAT GCAGTTCATT 
GTCGGTATCG AGCTGATTGG TACCCTTGTG TTGCCTGCCG CCATTGCCTT TACTTTCTAT 
GTTGTCATCA TCTCAATCAT CAACTCCCCG CCACAAATCA TTCCTCTTGT TCTCCTTGGT 
CTCATCCTCG GTCTCCCAGC CATCCTCGTC GTCGTCACAG CTCACAGCTG GTCCTACATC 
ATCTGGATGT TTATCTACTT GCTCTCCCTG CCCGTGTGGA ACTTTGTTCT GCCTACCTAC 
GCTTTCTGGA AGTTTGACGA TTTCTCATGG GGCGACACGC GCAAGACGGC CGGCGAGAAA 
TCAAGCAAGG GTGGCCACGG AGAAGCCGAG GGAGAGTTTG ATAGCAGCAT GATTACCATG 
AAGCGCTGGG CCGAGTTTGA GCGCGACAGG CGCGTCCGCA GCACGTACTG GGCCGGCTCG 
AGGGACAATG TCATATCCGG GGTAGGGGGT AGTAATGGGT GGGGAAGCAG CCAGCCGAGA 
GGCCATGAGC AGGGGAGACA CTTTGATGAC TACTTTTCGG ACGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393275.1
CDS	609..4316
Translation	MSLPRRPGPSPPHEEPRRYRQSGSRRSRPPPADVETGYAPMAGERPSQQQRVPSISSFPETLPSPNPNVERDPLAPTPEPAHPSGIPQRKRSLIRPERNRIGKDHPNYHYRKHAANMNTLPSSTGHDPIYEDLEGATDDVSGTGSRNDDDVSEESPPRRKHSTKMQVIETEKSGDERRRRRKSDTTKHGKIVKASKGKREKSGGLPTPSFWNIYCGFVTFWCPGFVLKCFGMPEMAQQRAWREKMGLISIILLIMGFVGFITFGFTQVVCGKPPLRLRINEVGSGYMIFHGSAYDLTKSHHPPAEGIPRRPDGLGANVIYDLPQHYGGQDGSFLFQNVNGKCKGLITKQENSDVPSDKSGNLAWYFPCNTFNQDGSSKPNTTIPYYLGYACHTTANARDSFYLGLKSSADVYFTWDDIKNSSRNLVVYSGHVLDLDLLHWFNDTQVTYPARFKELRDKNTAGNQAIRGRDITHAFQSSKDKQIAECFEEIIKVGSVDTETVGCIASKVVLYVSLVLILAVVLARFVLALIFQWFISKTYAAAKTSQTSDQRKRNRQIEDWTEDIYRAPPRLPGEVGSSVAGSSDRQSKRSSAFLPTHSRFSTVYGNERGNRKPGLPTTMASQNAAGQLLHPGTIYGQGNESRSSFLKSDAYGSSSSPADGPGPAGFIHEAVVPQPPSDWMPFGFPLAHTICLVTAYSEGEMGVRTTLDSIAMTDYPNSHKVILVICDGIIKGHGEEHSTPDIILGMMKDHTIHPDDVEPFSYVAVATGSKRHNMAKVYTGFYDYGTNSAIPLEKQQRVPMMMVVKCGTPAEASKSKPGNRGKRDSQIILMSFLQKVMFDERMTELEYEMFNGLWKITGISPDFYEIVLMVDADTKVFPDSLTHMISAMVKDPEIMGLCGETKIANKRASWVSAIQVFEYFVSHHLAKAFESVFGGVTCLPGCFCMYRIKAPKGAQNYWVPILANPDVVEHYSENVVDTLHKKNLLLLGEDRYLSTLMLRTFPKRKQVFVPQAVCKTTVPDEFMVLLSQRRRWINSTIHNLMELVLVRDLCGTFCFSMQFIVGIELIGTLVLPAAIAFTFYVVIISIINSPPQIIPLVLLGLILGLPAILVVVTAHSWSYIIWMFIYLLSLPVWNFVLPTYAFWKFDDFSWGDTRKTAGEKSSKGGHGEAEGEFDSSMITMKRWAEFERDRRVRSTYWAGSRDNVISGVGGSNGWGSSQPRGHEQGRHFDDYFSDA

Target ORF information:

RefSeq Version	XM_011394973.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A chitin synthase 4 (chs-4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394973.1

ORF Insert Sequence:

ATGTCTCTTC CTCGGAGGCC GGGGCCGTCG CCTCCGCACG AGGAGCCACG CCGCTACCGG 
CAAAGCGGTT CGCGCCGCAG TCGACCACCA CCCGCCGATG TTGAGACCGG ATATGCTCCC 
ATGGCAGGAG AAAGACCAAG CCAGCAGCAA CGCGTACCAT CGATATCTTC ATTTCCGGAA 
ACCCTCCCGT CCCCCAACCC CAATGTCGAA CGAGACCCTT TGGCGCCCAC CCCCGAGCCC 
GCACATCCAT CCGGCATACC TCAGCGGAAG AGGAGTTTGA TTCGACCGGA GCGCAACAGG 
ATTGGCAAAG ACCACCCCAA CTATCACTAC AGAAAACATG CGGCCAACAT GAACACCCTA 
CCGTCCTCAA CCGGACACGA CCCGATATAC GAGGATCTGG AGGGAGCAAC GGACGATGTA 
TCGGGAACTG GCTCGAGGAA CGACGACGAT GTTTCCGAGG AGTCGCCCCC AAGAAGAAAG 
CACTCGACGA AAATGCAGGT CATCGAAACT GAAAAGTCCG GTGATGAAAG ACGACGTCGG 
CGGAAATCGG ATACCACAAA ACATGGTAAA ATTGTCAAGG CCTCCAAGGG AAAGCGCGAA 
AAGTCCGGAG GACTCCCTAC CCCGAGCTTT TGGAACATTT ACTGCGGATT CGTCACCTTC 
TGGTGCCCTG GCTTTGTCCT CAAGTGCTTC GGCATGCCCG AGATGGCTCA ACAGAGGGCG 
TGGAGAGAGA AGATGGGTCT GATCAGCATT ATTCTGCTCA TCATGGGCTT TGTTGGCTTC 
ATCACTTTCG GCTTCACCCA GGTGGTCTGC GGTAAACCTC CGCTCCGTTT GCGCATCAAT 
GAGGTCGGGT CAGGATACAT GATCTTCCAC GGATCTGCAT ATGACCTCAC CAAATCTCAC 
CATCCTCCGG CCGAGGGTAT CCCGCGCCGT CCGGACGGTT TGGGTGCAAA CGTGATCTAT 
GACTTGCCTC AGCATTACGG TGGACAGGAC GGCAGTTTCT TGTTCCAGAA CGTCAACGGC 
AAGTGCAAGG GTCTCATCAC CAAGCAAGAG AACTCCGATG TGCCAAGCGA TAAGAGCGGC 
AATCTGGCGT GGTACTTCCC TTGTAACACG TTCAACCAAG ATGGTTCATC CAAGCCCAAT 
ACCACGATAC CTTATTACCT GGGCTATGCC TGTCACACGA CCGCTAACGC CAGGGACTCT 
TTCTATCTCG GCCTCAAGAG CTCCGCCGAT GTCTACTTTA CGTGGGATGA CATCAAGAAC 
AGCTCTCGAA ACCTTGTTGT GTACTCTGGC CATGTTCTCG ACCTTGATCT GCTTCACTGG 
TTCAACGACA CTCAAGTCAC GTATCCCGCC CGCTTCAAGG AGCTGAGGGA TAAGAATACT 
GCTGGAAACC AGGCTATTCG TGGACGCGAT ATTACCCATG CTTTCCAGTC GAGCAAAGAC 
AAGCAGATTG CCGAATGCTT TGAGGAGATC ATCAAGGTCG GTTCGGTGGA TACCGAGACA 
GTTGGCTGCA TTGCGTCAAA GGTCGTTCTC TATGTGTCCC TCGTCCTTAT CCTCGCGGTC 
GTCCTCGCCA GATTCGTCTT GGCTCTAATC TTCCAATGGT TCATCAGCAA GACGTATGCG 
GCTGCTAAGA CCTCCCAAAC GTCTGACCAG CGAAAGCGAA ACCGTCAGAT TGAAGACTGG 
ACTGAAGACA TTTACAGAGC TCCCCCAAGG CTACCAGGGG AGGTCGGTAG CAGTGTCGCT 
GGAAGCTCCG ATCGTCAAAG CAAGCGCAGC AGTGCTTTCT TGCCCACACA CTCTCGTTTC 
TCCACCGTCT ACGGCAATGA GCGCGGCAAT AGGAAGCCTG GACTTCCTAC AACCATGGCC 
AGCCAGAATG CCGCTGGTCA ACTACTCCAT CCTGGAACCA TATACGGCCA AGGAAATGAG 
AGTCGGTCAA GCTTCCTCAA ATCTGATGCT TACGGATCAA GCAGCAGTCC AGCAGACGGC 
CCAGGTCCTG CCGGATTTAT TCATGAAGCT GTCGTTCCTC AGCCGCCTTC CGACTGGATG 
CCGTTTGGTT TCCCCCTCGC TCACACCATC TGCCTTGTCA CAGCCTACTC CGAGGGTGAG 
ATGGGCGTTC GTACCACTCT GGACTCGATT GCAATGACGG ACTACCCCAA CAGCCACAAG 
GTTATCCTCG TTATTTGCGA TGGTATTATC AAGGGCCACG GAGAGGAACA CTCAACCCCC 
GATATCATTC TCGGCATGAT GAAAGACCAC ACGATTCACC CCGATGACGT TGAGCCATTC 
TCTTATGTTG CGGTTGCAAC TGGTTCTAAG CGCCACAACA TGGCCAAGGT CTACACCGGC 
TTCTACGATT ATGGCACTAA CTCTGCCATT CCTCTGGAGA AGCAGCAGCG TGTTCCTATG 
ATGATGGTTG TGAAGTGCGG CACTCCTGCT GAAGCCTCCA AGTCGAAGCC TGGAAACCGT 
GGCAAGCGTG ACAGTCAGAT CATCCTTATG TCTTTTCTCC AGAAGGTTAT GTTCGACGAG 
CGTATGACGG AGCTAGAATA CGAGATGTTC AATGGCTTGT GGAAGATCAC GGGCATTTCT 
CCTGACTTCT ATGAGATCGT GCTCATGGTT GACGCTGATA CCAAGGTCTT CCCCGACAGT 
CTCACGCATA TGATCTCGGC CATGGTCAAG GATCCTGAAA TTATGGGTCT TTGTGGCGAG 
ACCAAGATCG CCAACAAGCG AGCAAGCTGG GTTTCCGCCA TCCAGGTATT CGAGTATTTC 
GTCTCTCATC ATCTTGCCAA GGCTTTCGAG TCCGTCTTTG GCGGTGTCAC CTGTCTTCCC 
GGATGTTTCT GCATGTACCG CATCAAGGCA CCCAAGGGTG CCCAGAACTA CTGGGTCCCT 
ATCTTGGCCA ACCCGGACGT TGTCGAACAC TACTCCGAGA ACGTTGTCGA TACGCTGCAC 
AAGAAGAACC TCTTGCTCCT TGGTGAGGAT CGTTATCTCA GTACGCTCAT GTTGCGCACG 
TTCCCCAAGC GTAAACAAGT ATTTGTGCCT CAGGCCGTTT GCAAGACGAC TGTCCCCGAT 
GAATTCATGG TGTTGCTTTC GCAGCGCCGC AGATGGATCA ACTCGACCAT TCACAATCTG 
ATGGAGCTCG TTCTTGTGCG CGATCTTTGC GGTACCTTCT GTTTCAGTAT GCAGTTCATT 
GTCGGTATCG AGCTGATTGG TACCCTTGTG TTGCCTGCCG CCATTGCCTT TACTTTCTAT 
GTTGTCATCA TCTCAATCAT CAACTCCCCG CCACAAATCA TTCCTCTTGT TCTCCTTGGT 
CTCATCCTCG GTCTCCCAGC CATCCTCGTC GTCGTCACAG CTCACAGCTG GTCCTACATC 
ATCTGGATGT TTATCTACTT GCTCTCCCTG CCCGTGTGGA ACTTTGTTCT GCCTACCTAC 
GCTTTCTGGA AGTTTGACGA TTTCTCATGG GGCGACACGC GCAAGACGGC CGGCGAGAAA 
TCAAGCAAGG GTGGCCACGG AGAAGCCGAG GGAGAGTTTG ATAGCAGCAT GATTACCATG 
AAGCGCTGGG CCGAGTTTGA GCGCGACAGG CGCGTCCGCA GCACGTACTG GGCCGGCTCG 
AGGGACAATG TCATATCCGG GGTAGGGGGT AGTAATGGGT GGGGAAGCAG CCAGCCGAGA 
GGCCATGAGC AGGGGAGACA CTTTGATGAC TACTTTTCGG ACGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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chs-4 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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