gh47-7 cDNA ORF clone, Neurospora crassa OR74A

The following gh47-7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gh47-7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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ONf12530 XM_958635.2
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Neurospora crassa OR74A ER glycosyl hydrolase (gh47-7), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf12530
    Clone ID Related Accession (Same CDS sequence) XM_958635.2
    Accession Version XM_958635.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product ER glycosyl hydrolase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_958635.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACACGGG AACCACTGCG GCCCCGACTC CGGCCCCGAT CATCACCATG GCTTCTTGCT 
    CTGGCTGTGT TGGCCTTTGT GGTCCTGCTG CCCAGTGCTG GGGCAATGCG CCCCAGTCGC
    GTGGCTGAGC TCCGCCGTGA GGTCGTCGAC ATGTTTTACC ATGGCTTCGA TAACTACATG
    CGCATCGCCT TTCCCGAGGA CGAGCTCCGG CCCGTCACTT GCGCCCCTCT AACCCGGGAC
    GCTGAGAACC CAGGAAACGT CGAAGTGAAC GATGTCTTGG GTAACTACTC CCTTACTTTG
    ATAGACAGTC TGTCTACCTT GGCCATCTTC GCTTCGGCGC CGCCAGATGA CCGTGGGACG
    GGACCGAAAG CTTTGCGTGA CTTCCAGGAT GGTGTTGCCG CCTTGGTCGA GCAATATGGC
    GACGGCCGTC CCGGTCCTTC TGGCCAAGGC CTGCGCGCTA GAGGCTTCGA TATCGACAGC
    AAGGTGCAGG TTTTTGAAAC GGTCATCCGT GGTGTTGGCG GCCTCCTCAG TGCCCATTTG
    TTTGCTGTTG GCGCACTACC GATTAATGGA TATGAACCGC TTGGTCCTGA ACATGATCCA
    CTAAGCCCTC CGCCCATCCA CTGGCCGAAT GGCTTGACCT ACGATGGCCA GCTGCTTCGT
    TTGGCGCTTG ATCTTGGGAC CAGGATTCTC CCGGCCTTTT ACACGAAAAC GGGAATGCCC
    TATCCGCGTG TCAATCTGCG CCATGGTATC CCCTTTTATA AGAACTCGCC CTTGCATGGG
    ACGTTTTCCG GGGACCACAT AGTTGATGGG CCTCCTGAGA TTACCGAGAC ATGCAGTGCT
    GGTGCCGGGT CCCTTGTTCT TGAATTCACG GTCCTCAGTC GCTTGACAGG TGATCCGCGG
    TTTGAACAGC TGGCCAAGCG AGCCTTTTGG GCAGTTTGGT ATAGGAAGAG CCACATAGGC
    CTCCTCGGCG CAGGCGTCGA TGCTGAACAA GGCCATTGGG TAGGCCCTTA TTCCGTCGTT
    GGTGCTGGCG CCGACAGCTT TTTCGAGTAC GCCCTCAAAT CGTACATCTT GCTTTCTGGG
    CACGAGCTTC CAAACCGGAC AGCCGTTGAG CCAAAACCCA AGGACAGCTG GCTGGACCCC
    AATACTCTCT TTGTTCCACT TACTGATGTT GAGAATTCCC CTGATGCCTT TCTTCAGGCG
    TGGCATCATG CCCATGCTGC TATCAAGCGC CATCTTTACA GCGATCGAGA TCATCCGCAT
    TATGAAAACG TCAACCTATG GACAGGCTCG CTGGCTACTA ACTGGGTAGA CAGCCTAGGT
    GCCTTCTATG CCGGTTTGCT CGTCCTGGCT GGTGAGCTGG AAGAAGCCAT TGAAACGAGC
    TTGTTGTACA CTGCCATATG GGCGCGCTAT GCCGCGCTTC CTGAACGTTG GTCTATTACA
    CACAAGGATA TTGAGGGTGG TCTAGGCTGG TGGCCGTTGC GACCCGAGTT TATCGAGTCG
    ACCTATTACA TATATCAAGC CACCAAGGAC CCCTGGTATC TTTACGTTGG CGAAATGGTC
    ATGAGAGACA TTCAGAGACG ATGTACTACG CAGTGTGGCT GGGCAGGCCT TCAAAATGTG
    TTGACCGGCG AGAGGAGCGA TCGTATGGAA AGCTTTTTCC TCGGCGAAAC CACGCAGTAC
    CTGTATCTGC TGTTTGATGA GTCGCATCCC CTCAACCACC TTGATGCCGG TTTTGTGTTT
    ACCACAGAAG GTCATCCGCT AGTTTTGCCA AAGGCAAAGC CAAGGACCCG ATCACGAGCT
    TCTGGTCAAA AGGAATTGAT AGTGTATCAC GACCCTGGCT TTACTAACAC TTGCCCGGCA
    CCGCCTTCTG TCACTCCCCT CACAGGCTCA GCCATCGCTG CCAGAGATGA CATCTATCAC
    GCTGCAAAGA TGTTGGACCT ACACTTGTTG CAGCCAAAGA ACTTTTTAGG ACTTCCGGCA
    GAGGGTAGTA AGCCCTCCGG CCAACATACA TCTTCTAGGA AGCCTATAAC CGAATCGGAC
    CAGTTCACGC CTTTCCCCTG GTCTCTTCCA CCAGAAATGG TTCCACAAAA CGGAACGTGC
    CGCAAAATCA ACCAGCCGGT CGAGATGCTG CTCGAGTTCT CCTCCAACGC GCAAGAACTC
    ATTGGCGGCA GCGCCTTCAA CTACCTGATG GGCACGCAGA ACCTCGAGCG CCTCACGATG
    GACCGAATCC GCGTTCAAAC CCTTTCCGGC TTGAGGCTGA CCATGCGGCA GGAGGAGGAC
    TCGGACGGTG AATGGCGTGT CAGCAAGGTG AATGGCGTCC TTCTCGGTCG GGATGAGTTC
    ATCGTTATGA ACCGAGCCAT CCTAGGCGAG GTGTCCGACC CGCGGTTCAA CCTCGTTCGC
    GACCCCGTCA ACGTCAAGCT CGTGCATCTA CATCATGTGG ACCTAGGCCA GGACGAGACC
    CACAAGGACA AGGCGAAGGG GCAGCTTCAC CCCGAAAGCG TGAAGACTGA TCCTGAGGTT
    TCCTCCCAAG AGGAGCAGGA GAAGGAGATC GAAGAACCAC AGACAGAAAC AGCCAACACC
    ACCTCGGAAG ACCCCGCCTC CTCCTCCCTC GGCTCCTACG TCAAATCCCT CCTCGCCAAC
    CTCGCCGCCT CCCTCGACGA CCTGCTCGAC GGCATCCCCC TCGAAGCAGC CGTCACCGCT
    CTCCCTTCCA TCCTCCCCTC CATCCCCTCC ATCTCATCCA TCCATGGCGG CAGCACCAAA
    CGCATCAAAT ACCCTCTCAA CGTCTTCATA AACTCCACCG CCGTAACCGC CACGGGCATC
    GGTGCCGCCC CTCTCCCTCC TCCTCCCCCT CGCCCCTCAT CTCCATACAT CTCCAGCGGA
    AACAACCTTG CTCATCCGCG ACCCTACCCG CCCTTCGGCC CCGTCCCCCG CGACATGATT
    CCCTACACCA CCATCTATGC CGCCGGCCAG CTTTGTACCT CGTCCTCGTC CGGTTCTTCC
    GACGAGCCTT CTTCTTCTGA AAAGGACCAG TCTTCGCCGC CGCCTCCACT ACTAGACGAC
    TCCATCCCGC GCACCCACCA GATCCTCCTC CTCCGCCGCG GCGGCTGTTC CTTTTCCGAG
    AAACTCGCCA ACATCCCCGC CTTCCCTCCC TCTCCCACCT CGCTCCAACT CGTAATCGTC
    GTTTCCGACG GCCAAGACGA AGATGCTTCG TACGCTGCTT ACTACTCTGC TGGTCAAACT
    ACCTACAGCA GCAGCAGCAG CCAAGGAGGT GGAGGTGGGG TTGGAGTTGG AGGTGGGGGG
    TTAGCTAGGC CCTTGTTGGA TCAGGTACAA AAGACTCCTG GCGGACTGGC GAGGAGGAAT
    CCGATTCCGA TGATCATGGT CGGGGGTGGA GAGATGACGT ATGAAACGCT CAAAAGGGCC
    AAGGGCGTGG CGGTGGGGCG GAGGTGGTTT TTGGAAAGTC AGGGGCGGAG AGTGAGGAAT
    GTGATTGTGG ACGAAGGGGA TGCTGGGGGG GTTGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_963728.2
    CDS271..3789
    Translation

    Target ORF information:

    RefSeq Version XM_958635.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A ER glycosyl hydrolase (gh47-7), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_958635.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACACGGG AACCACTGCG GCCCCGACTC CGGCCCCGAT CATCACCATG GCTTCTTGCT 
    CTGGCTGTGT TGGCCTTTGT GGTCCTGCTG CCCAGTGCTG GGGCAATGCG CCCCAGTCGC
    GTGGCTGAGC TCCGCCGTGA GGTCGTCGAC ATGTTTTACC ATGGCTTCGA TAACTACATG
    CGCATCGCCT TTCCCGAGGA CGAGCTCCGG CCCGTCACTT GCGCCCCTCT AACCCGGGAC
    GCTGAGAACC CAGGAAACGT CGAAGTGAAC GATGTCTTGG GTAACTACTC CCTTACTTTG
    ATAGACAGTC TGTCTACCTT GGCCATCTTC GCTTCGGCGC CGCCAGATGA CCGTGGGACG
    GGACCGAAAG CTTTGCGTGA CTTCCAGGAT GGTGTTGCCG CCTTGGTCGA GCAATATGGC
    GACGGCCGTC CCGGTCCTTC TGGCCAAGGC CTGCGCGCTA GAGGCTTCGA TATCGACAGC
    AAGGTGCAGG TTTTTGAAAC GGTCATCCGT GGTGTTGGCG GCCTCCTCAG TGCCCATTTG
    TTTGCTGTTG GCGCACTACC GATTAATGGA TATGAACCGC TTGGTCCTGA ACATGATCCA
    CTAAGCCCTC CGCCCATCCA CTGGCCGAAT GGCTTGACCT ACGATGGCCA GCTGCTTCGT
    TTGGCGCTTG ATCTTGGGAC CAGGATTCTC CCGGCCTTTT ACACGAAAAC GGGAATGCCC
    TATCCGCGTG TCAATCTGCG CCATGGTATC CCCTTTTATA AGAACTCGCC CTTGCATGGG
    ACGTTTTCCG GGGACCACAT AGTTGATGGG CCTCCTGAGA TTACCGAGAC ATGCAGTGCT
    GGTGCCGGGT CCCTTGTTCT TGAATTCACG GTCCTCAGTC GCTTGACAGG TGATCCGCGG
    TTTGAACAGC TGGCCAAGCG AGCCTTTTGG GCAGTTTGGT ATAGGAAGAG CCACATAGGC
    CTCCTCGGCG CAGGCGTCGA TGCTGAACAA GGCCATTGGG TAGGCCCTTA TTCCGTCGTT
    GGTGCTGGCG CCGACAGCTT TTTCGAGTAC GCCCTCAAAT CGTACATCTT GCTTTCTGGG
    CACGAGCTTC CAAACCGGAC AGCCGTTGAG CCAAAACCCA AGGACAGCTG GCTGGACCCC
    AATACTCTCT TTGTTCCACT TACTGATGTT GAGAATTCCC CTGATGCCTT TCTTCAGGCG
    TGGCATCATG CCCATGCTGC TATCAAGCGC CATCTTTACA GCGATCGAGA TCATCCGCAT
    TATGAAAACG TCAACCTATG GACAGGCTCG CTGGCTACTA ACTGGGTAGA CAGCCTAGGT
    GCCTTCTATG CCGGTTTGCT CGTCCTGGCT GGTGAGCTGG AAGAAGCCAT TGAAACGAGC
    TTGTTGTACA CTGCCATATG GGCGCGCTAT GCCGCGCTTC CTGAACGTTG GTCTATTACA
    CACAAGGATA TTGAGGGTGG TCTAGGCTGG TGGCCGTTGC GACCCGAGTT TATCGAGTCG
    ACCTATTACA TATATCAAGC CACCAAGGAC CCCTGGTATC TTTACGTTGG CGAAATGGTC
    ATGAGAGACA TTCAGAGACG ATGTACTACG CAGTGTGGCT GGGCAGGCCT TCAAAATGTG
    TTGACCGGCG AGAGGAGCGA TCGTATGGAA AGCTTTTTCC TCGGCGAAAC CACGCAGTAC
    CTGTATCTGC TGTTTGATGA GTCGCATCCC CTCAACCACC TTGATGCCGG TTTTGTGTTT
    ACCACAGAAG GTCATCCGCT AGTTTTGCCA AAGGCAAAGC CAAGGACCCG ATCACGAGCT
    TCTGGTCAAA AGGAATTGAT AGTGTATCAC GACCCTGGCT TTACTAACAC TTGCCCGGCA
    CCGCCTTCTG TCACTCCCCT CACAGGCTCA GCCATCGCTG CCAGAGATGA CATCTATCAC
    GCTGCAAAGA TGTTGGACCT ACACTTGTTG CAGCCAAAGA ACTTTTTAGG ACTTCCGGCA
    GAGGGTAGTA AGCCCTCCGG CCAACATACA TCTTCTAGGA AGCCTATAAC CGAATCGGAC
    CAGTTCACGC CTTTCCCCTG GTCTCTTCCA CCAGAAATGG TTCCACAAAA CGGAACGTGC
    CGCAAAATCA ACCAGCCGGT CGAGATGCTG CTCGAGTTCT CCTCCAACGC GCAAGAACTC
    ATTGGCGGCA GCGCCTTCAA CTACCTGATG GGCACGCAGA ACCTCGAGCG CCTCACGATG
    GACCGAATCC GCGTTCAAAC CCTTTCCGGC TTGAGGCTGA CCATGCGGCA GGAGGAGGAC
    TCGGACGGTG AATGGCGTGT CAGCAAGGTG AATGGCGTCC TTCTCGGTCG GGATGAGTTC
    ATCGTTATGA ACCGAGCCAT CCTAGGCGAG GTGTCCGACC CGCGGTTCAA CCTCGTTCGC
    GACCCCGTCA ACGTCAAGCT CGTGCATCTA CATCATGTGG ACCTAGGCCA GGACGAGACC
    CACAAGGACA AGGCGAAGGG GCAGCTTCAC CCCGAAAGCG TGAAGACTGA TCCTGAGGTT
    TCCTCCCAAG AGGAGCAGGA GAAGGAGATC GAAGAACCAC AGACAGAAAC AGCCAACACC
    ACCTCGGAAG ACCCCGCCTC CTCCTCCCTC GGCTCCTACG TCAAATCCCT CCTCGCCAAC
    CTCGCCGCCT CCCTCGACGA CCTGCTCGAC GGCATCCCCC TCGAAGCAGC CGTCACCGCT
    CTCCCTTCCA TCCTCCCCTC CATCCCCTCC ATCTCATCCA TCCATGGCGG CAGCACCAAA
    CGCATCAAAT ACCCTCTCAA CGTCTTCATA AACTCCACCG CCGTAACCGC CACGGGCATC
    GGTGCCGCCC CTCTCCCTCC TCCTCCCCCT CGCCCCTCAT CTCCATACAT CTCCAGCGGA
    AACAACCTTG CTCATCCGCG ACCCTACCCG CCCTTCGGCC CCGTCCCCCG CGACATGATT
    CCCTACACCA CCATCTATGC CGCCGGCCAG CTTTGTACCT CGTCCTCGTC CGGTTCTTCC
    GACGAGCCTT CTTCTTCTGA AAAGGACCAG TCTTCGCCGC CGCCTCCACT ACTAGACGAC
    TCCATCCCGC GCACCCACCA GATCCTCCTC CTCCGCCGCG GCGGCTGTTC CTTTTCCGAG
    AAACTCGCCA ACATCCCCGC CTTCCCTCCC TCTCCCACCT CGCTCCAACT CGTAATCGTC
    GTTTCCGACG GCCAAGACGA AGATGCTTCG TACGCTGCTT ACTACTCTGC TGGTCAAACT
    ACCTACAGCA GCAGCAGCAG CCAAGGAGGT GGAGGTGGGG TTGGAGTTGG AGGTGGGGGG
    TTAGCTAGGC CCTTGTTGGA TCAGGTACAA AAGACTCCTG GCGGACTGGC GAGGAGGAAT
    CCGATTCCGA TGATCATGGT CGGGGGTGGA GAGATGACGT ATGAAACGCT CAAAAGGGCC
    AAGGGCGTGG CGGTGGGGCG GAGGTGGTTT TTGGAAAGTC AGGGGCGGAG AGTGAGGAAT
    GTGATTGTGG ACGAAGGGGA TGCTGGGGGG GTTGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B