dcl-2 cDNA ORF clone, Neurospora crassa OR74A

The following dcl-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dcl-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf12475 XM_958445.3
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Neurospora crassa OR74A dicer-like protein 2 (dcl-2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf12475
    Clone ID Related Accession (Same CDS sequence) XM_958445.3
    Accession Version XM_958445.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4191bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product dicer-like protein 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502). On Feb 23, 2015 this sequence version replaced XM_958445.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCTCTGTG GCCAGGATGG GGTGGACAGC TGGTCTGAGC AGGCTGTCTG GGATGCCGTC 
    TTGCTCAACG TTCGTATCGT GGTGTCGACA TACCAGATCT TGTTCGATGC AAATGCCCAC
    AGCTTCGTCC GCTTGGACTC CTTGAGCTTG ATTGTCATCG ATGAAGCACA CAATTGCAGC
    GGGAGCCATC CCATTGCCAG ACTGATGACC GAGGCGTACC TCCCTGCAAA GAAGGCTGGT
    CTACCAGTCC CGAGCATCCT CGGTCTTACG GCAAGCCCGC TAAAGTCAAA TAACCTCGCT
    GACATTGAAA AGCTTGAACA AGTCCTTGAT GCAGTCTGTA GGACTCCTAC GATACATCGA
    GAAGAGCTTC TTGCACACGT GAACCGCCCG GAGATGCTCG TTGTTTCATA CGGCGACTCT
    GGTACCGATC CAACACCAAC CGATCTCATG ACAAGATTCC TAGAAGCATA CCACAGGCTT
    GATATAAGCA AGGACCCAGA TGTCTTGTTG CTCAAAGCAC AACGCACCGA ACGGGCTAGG
    GAGAAGCTTC GTCAGATGAT TACCAAGAAG GACACGCTCG CCCAGAAAGA ATTGAGAGGG
    GTTTACAACA GGGCCCTCCT TGTGCGTCGG GAAATTGGGC CATGGGCGGC AGACTATTAC
    CTCACAAGGA CAGTTTCGCA TATGTTGGCC GAACTGGAGC GCGGGGAACC TCCGGCCCAA
    CACCGGTACA TAGGCGAGGC ATTACGATCT ATCCCCATTC CGGCTATTTC AAAAGAGCCC
    ATCCAGCTCT CCCCAAAAGT CCAGACTTTG CTCAAAGTCC TTGCTTCCCA TCAACAGGAC
    CCGGTTGGTA TTGTTTTTGT CAAGGAGAGG GTAATGGTCA GCATTGTGAC GCATATCATA
    TCCACCCATC CGCTCACCAA GGACCGTTAC CGCACTGCTT CCATGATTGG CACCGCTAGT
    GTGCCAGGAA AGGCGAGGAA TCACATGGAC ATGACAAAAA AGGAGGACAT GACATCCCTC
    GAAGGATTTC GCCTAGGCCG CTTCAATCTA CTTGTCGCAA CGAGTGTCCT CGAGGAGGGC
    ATCGACGTTC CCATTTGCAA CCTCGTCATT TGTTTTGACG AACCATCAAA CATCAAATCC
    TTTATTCAGC GTCGCGGAAG GGCAAGAGAG GTAAGCTCCA CTCTCTATCT AATGGTTCAA
    AACGCCTCTA GCGAGTCCGC AACGGACTGG CATAATCTGG AACGGCTGAT GAAAGAGAGG
    TACGAGGACG AGATGCGTCA AAACGCAGAA CTCGAGTTGC TCGATGATCC GAGAATTGGC
    AGTTATCCGG TTCTGGAAGT CGAGAGTACT GGCGCTCGCA TGACTATCAG AGATGCCAGA
    TCCCATCTCA ATCACTTTTG CGCAAAGGTC TCGTCAAGAT CCCGATATCT TCAGAAGGAA
    CCGTACTTCG TCATTCGTCA AGTCAATCCG GACCCAGCAT CACCTGGTCG ACGAACATTG
    CTCCAGGCAA CTGTTCATCT GCCAGCATCT CTTGCTCCTG ATTTACGTCG CCACGAAAGT
    CTTTGGACTT GGACGTCGGA GAAGCTTGCA ATCATGGATG CCTCATTTCA GGCATATAAA
    GCTCTTTACA ACGCAGGATT AGTCAATGAG AATTTGCTGC CTACAAAGGT ATCTGACTTC
    CTGGCCGACC TTGGGGACGA CCCGGGTCAT ATTTGGGTCA AGACACAGTT CGACCCCTGG
    CCAGAGGTAG CTTATGCCTG GCAAGAGAGT TCGTCCCTAT ACAGTCGTCG CCTGACAGTT
    CTGGTTCCAG GTGTAGAAAA CCCCCTCGAA TTCGAATTTA TACTGCCCGT GCCAGTTCCT
    TACATGGCAC CTTTAAAGCT GTGGTGGAAT GCCACTTCTG CTTTAACGCT CATCACCAGT
    CCAGAGATGC AGGAATTCCG CAAACAAGAA GGAACCAGTG CTGGGCCAGA CCATTCATAT
    GCTTTGCTCG CCATGGCTTT TGCACATCGA TTCCCAATCC AGGGCAGGCA ATATCCGATT
    CGTCTTGTAT CAACCCGAAG GAAACTTGAC GTCGACGGAA TTGCAGCTCT CGAGTTTGAC
    CCTCGACTGT ATGAAAGCTC CCCTCAGCCT CCCCTTGTGA GGCTCGTAGA CGGGAGGAAC
    ATGCCCTATT TCGTCACCAA GATCTTGCCC TCCAAGCCAC CGGTCGAACT CATATCCAAG
    CCGAGTACAG ATCATGCAGA CCTTCCAGAG AACGTTCCTT ACGTGGTTTG CAAGCCCATT
    GGCAAAGCCG TGGGTCAGTT CATTCCACTT GATGCAGCTC AAGACCAGGA CAGTTGGACT
    CCGAAGAATG GAAAGCTGTA CCGAAAGGTC CTTCCCTCCA CACAGATTCG GATGGATAAC
    TTCCCGGCCG TGTTTGCTCA GGTGGGCGCG GTCATCCCAG CTTTCACAAG GGCAGTGGAA
    ATGAGTTTAG TGGCCGCGGA CCTGATGTAC AATCGTCTGG GTTGTTTGCA ACTTGATAAC
    TTGCCTCTCA TTACGACCGC ACTCATCAGC AGTGGGTCTC GAGGCCCGAC CAACTACGAA
    AGATTGGAGT TTATCGGCGA TACAATCTTA AAGTTCTGCG CCTGCCTCAC TGCTTCGGCA
    TTATTTCCAA ACCACCATGA ACGGTTACTT TCCCAATGGA AAGACAAGCT TGTGAACAAC
    GTGCGACTCT GTCGGGCATC TCGCGATTTT GGCCTTGACG AGTACATCAT CAACTCTGCG
    GCCTCGAAGA AGTGGCGGCC CAAGTTTGTC GAAGATTATA TGGATGAGAT GAAGTCCCCG
    ATATCGGCAG AAACCCGGCA GATGAGCTCC AAGATGGTAG CCGATGTTGT CGAGTCCCTC
    ATCGGCGCCG CATACATGTG TGGGGGCATG TCAAAGGCAC TCGAGTGCGT CGCCCTCCTT
    CTCCCCACTC CCAAGTCCAG CCAGTTCAAG TGGCAGGAAA TCGAGCTTAG TCGTACCCAG
    CTTTTTGAGT TTGCTCCCAA GGATGCCATC CTTTCCAAGC AATTAGAGCC TCTAGAAAAG
    GCCATGGACT ACACGTTTAA CAAAAAGTCA CTCCTAATCG AGGCCATGAC GCACCCGTCC
    TGCGCTGGTC TCGGTACCAA CGAATCCTGC TACGAACGTC TCGAGTTCCT CGGCGACGCC
    ATCCTCGACG TCATCGTCGT CAAGCGTCTC ATGGCCGAGA CAGGCCCCAA CGAGCTCGCG
    CACAATGACA TGCACGAGCA CCTCTCCTCC GTCGTCACCG CCGACATCAT GGCTTTCCTG
    GCCATGGAAT GGGTCATCAT GCAGACGGAC ATCAACGAGA TCGACCCTAC TAATCTGGAC
    GCCCTCGGCC TCCTTCCCTC ATCCCAAAGC AGGATAACAC CTGCGAGCCT GGTTTCCAAC
    AAGGAGGATT GGCCCTTTTG GCGATTCATG CGCCACAACT CGCCACAGGT CGGAGCCACC
    CAGACCGCCA CCATAGAACG CTACCTCACC CTCAGAGACG AGATTCGCGA TGCGATCTGG
    AAGCACAACA CGCTGCCGTG GGCCCTGCTG GCGCGCATGG GCCCCCAGAA GTTCTACTCA
    GACATTGTCG AATCGCTCAT TGGAGCCGTG TGGGTCGACT CCGGCTCGTG GAAAGCATGC
    GAGGACGTGC TGACCCAAAT GGGCCTCTTG CCTCTGCTGG ATCACTTACT CGAGACAAAG
    GCGCATGTGA TGCATCCCAA TGTCGAACTG CAGATCCTGG CGCCCCCGAA CAAGCGGGCG
    ACGCGCACCG AGTTTGTGAT CATCTCTAAC AAGAGAGGTA TCATCAGCTC CGGGACGGAG
    TTTCTGGATG AGCCGAGTGC CGTTGACGAT GGGCTGGTGT CGGTGGAGCC ATATGACGAT
    ACTCCGGAAC ATGACGAGGT GTTTTCTTGC AAGCTCTTTG TTGGAGGGAA GCAGGTGGCT
    GACGTGACGG GAGCGGCGAC GAAGGAGGAG GCCAGGGTAA GGGCGGCGGA GAAGGGGTGC
    TTGGTGATCA AGGCGGAAAG GAAGGTATGG AACGAAGCGA AAGCTGCTGC TAAAGAGGAT
    AAGGGGCATA ACACGGAGAA TGGAGACGCC AACGCGGATA ATGGGCAGAG CGGGGAAAAG
    GAAGAGGTCC CTGATTGCCG TGATGCGGAT GGGGATACAG TCATGAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_963538.3
    CDS971..5161
    Translation

    Target ORF information:

    RefSeq Version XM_958445.3
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A dicer-like protein 2 (dcl-2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_958445.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCTCTGTG GCCAGGATGG GGTGGACAGC TGGTCTGAGC AGGCTGTCTG GGATGCCGTC 
    TTGCTCAACG TTCGTATCGT GGTGTCGACA TACCAGATCT TGTTCGATGC AAATGCCCAC
    AGCTTCGTCC GCTTGGACTC CTTGAGCTTG ATTGTCATCG ATGAAGCACA CAATTGCAGC
    GGGAGCCATC CCATTGCCAG ACTGATGACC GAGGCGTACC TCCCTGCAAA GAAGGCTGGT
    CTACCAGTCC CGAGCATCCT CGGTCTTACG GCAAGCCCGC TAAAGTCAAA TAACCTCGCT
    GACATTGAAA AGCTTGAACA AGTCCTTGAT GCAGTCTGTA GGACTCCTAC GATACATCGA
    GAAGAGCTTC TTGCACACGT GAACCGCCCG GAGATGCTCG TTGTTTCATA CGGCGACTCT
    GGTACCGATC CAACACCAAC CGATCTCATG ACAAGATTCC TAGAAGCATA CCACAGGCTT
    GATATAAGCA AGGACCCAGA TGTCTTGTTG CTCAAAGCAC AACGCACCGA ACGGGCTAGG
    GAGAAGCTTC GTCAGATGAT TACCAAGAAG GACACGCTCG CCCAGAAAGA ATTGAGAGGG
    GTTTACAACA GGGCCCTCCT TGTGCGTCGG GAAATTGGGC CATGGGCGGC AGACTATTAC
    CTCACAAGGA CAGTTTCGCA TATGTTGGCC GAACTGGAGC GCGGGGAACC TCCGGCCCAA
    CACCGGTACA TAGGCGAGGC ATTACGATCT ATCCCCATTC CGGCTATTTC AAAAGAGCCC
    ATCCAGCTCT CCCCAAAAGT CCAGACTTTG CTCAAAGTCC TTGCTTCCCA TCAACAGGAC
    CCGGTTGGTA TTGTTTTTGT CAAGGAGAGG GTAATGGTCA GCATTGTGAC GCATATCATA
    TCCACCCATC CGCTCACCAA GGACCGTTAC CGCACTGCTT CCATGATTGG CACCGCTAGT
    GTGCCAGGAA AGGCGAGGAA TCACATGGAC ATGACAAAAA AGGAGGACAT GACATCCCTC
    GAAGGATTTC GCCTAGGCCG CTTCAATCTA CTTGTCGCAA CGAGTGTCCT CGAGGAGGGC
    ATCGACGTTC CCATTTGCAA CCTCGTCATT TGTTTTGACG AACCATCAAA CATCAAATCC
    TTTATTCAGC GTCGCGGAAG GGCAAGAGAG GTAAGCTCCA CTCTCTATCT AATGGTTCAA
    AACGCCTCTA GCGAGTCCGC AACGGACTGG CATAATCTGG AACGGCTGAT GAAAGAGAGG
    TACGAGGACG AGATGCGTCA AAACGCAGAA CTCGAGTTGC TCGATGATCC GAGAATTGGC
    AGTTATCCGG TTCTGGAAGT CGAGAGTACT GGCGCTCGCA TGACTATCAG AGATGCCAGA
    TCCCATCTCA ATCACTTTTG CGCAAAGGTC TCGTCAAGAT CCCGATATCT TCAGAAGGAA
    CCGTACTTCG TCATTCGTCA AGTCAATCCG GACCCAGCAT CACCTGGTCG ACGAACATTG
    CTCCAGGCAA CTGTTCATCT GCCAGCATCT CTTGCTCCTG ATTTACGTCG CCACGAAAGT
    CTTTGGACTT GGACGTCGGA GAAGCTTGCA ATCATGGATG CCTCATTTCA GGCATATAAA
    GCTCTTTACA ACGCAGGATT AGTCAATGAG AATTTGCTGC CTACAAAGGT ATCTGACTTC
    CTGGCCGACC TTGGGGACGA CCCGGGTCAT ATTTGGGTCA AGACACAGTT CGACCCCTGG
    CCAGAGGTAG CTTATGCCTG GCAAGAGAGT TCGTCCCTAT ACAGTCGTCG CCTGACAGTT
    CTGGTTCCAG GTGTAGAAAA CCCCCTCGAA TTCGAATTTA TACTGCCCGT GCCAGTTCCT
    TACATGGCAC CTTTAAAGCT GTGGTGGAAT GCCACTTCTG CTTTAACGCT CATCACCAGT
    CCAGAGATGC AGGAATTCCG CAAACAAGAA GGAACCAGTG CTGGGCCAGA CCATTCATAT
    GCTTTGCTCG CCATGGCTTT TGCACATCGA TTCCCAATCC AGGGCAGGCA ATATCCGATT
    CGTCTTGTAT CAACCCGAAG GAAACTTGAC GTCGACGGAA TTGCAGCTCT CGAGTTTGAC
    CCTCGACTGT ATGAAAGCTC CCCTCAGCCT CCCCTTGTGA GGCTCGTAGA CGGGAGGAAC
    ATGCCCTATT TCGTCACCAA GATCTTGCCC TCCAAGCCAC CGGTCGAACT CATATCCAAG
    CCGAGTACAG ATCATGCAGA CCTTCCAGAG AACGTTCCTT ACGTGGTTTG CAAGCCCATT
    GGCAAAGCCG TGGGTCAGTT CATTCCACTT GATGCAGCTC AAGACCAGGA CAGTTGGACT
    CCGAAGAATG GAAAGCTGTA CCGAAAGGTC CTTCCCTCCA CACAGATTCG GATGGATAAC
    TTCCCGGCCG TGTTTGCTCA GGTGGGCGCG GTCATCCCAG CTTTCACAAG GGCAGTGGAA
    ATGAGTTTAG TGGCCGCGGA CCTGATGTAC AATCGTCTGG GTTGTTTGCA ACTTGATAAC
    TTGCCTCTCA TTACGACCGC ACTCATCAGC AGTGGGTCTC GAGGCCCGAC CAACTACGAA
    AGATTGGAGT TTATCGGCGA TACAATCTTA AAGTTCTGCG CCTGCCTCAC TGCTTCGGCA
    TTATTTCCAA ACCACCATGA ACGGTTACTT TCCCAATGGA AAGACAAGCT TGTGAACAAC
    GTGCGACTCT GTCGGGCATC TCGCGATTTT GGCCTTGACG AGTACATCAT CAACTCTGCG
    GCCTCGAAGA AGTGGCGGCC CAAGTTTGTC GAAGATTATA TGGATGAGAT GAAGTCCCCG
    ATATCGGCAG AAACCCGGCA GATGAGCTCC AAGATGGTAG CCGATGTTGT CGAGTCCCTC
    ATCGGCGCCG CATACATGTG TGGGGGCATG TCAAAGGCAC TCGAGTGCGT CGCCCTCCTT
    CTCCCCACTC CCAAGTCCAG CCAGTTCAAG TGGCAGGAAA TCGAGCTTAG TCGTACCCAG
    CTTTTTGAGT TTGCTCCCAA GGATGCCATC CTTTCCAAGC AATTAGAGCC TCTAGAAAAG
    GCCATGGACT ACACGTTTAA CAAAAAGTCA CTCCTAATCG AGGCCATGAC GCACCCGTCC
    TGCGCTGGTC TCGGTACCAA CGAATCCTGC TACGAACGTC TCGAGTTCCT CGGCGACGCC
    ATCCTCGACG TCATCGTCGT CAAGCGTCTC ATGGCCGAGA CAGGCCCCAA CGAGCTCGCG
    CACAATGACA TGCACGAGCA CCTCTCCTCC GTCGTCACCG CCGACATCAT GGCTTTCCTG
    GCCATGGAAT GGGTCATCAT GCAGACGGAC ATCAACGAGA TCGACCCTAC TAATCTGGAC
    GCCCTCGGCC TCCTTCCCTC ATCCCAAAGC AGGATAACAC CTGCGAGCCT GGTTTCCAAC
    AAGGAGGATT GGCCCTTTTG GCGATTCATG CGCCACAACT CGCCACAGGT CGGAGCCACC
    CAGACCGCCA CCATAGAACG CTACCTCACC CTCAGAGACG AGATTCGCGA TGCGATCTGG
    AAGCACAACA CGCTGCCGTG GGCCCTGCTG GCGCGCATGG GCCCCCAGAA GTTCTACTCA
    GACATTGTCG AATCGCTCAT TGGAGCCGTG TGGGTCGACT CCGGCTCGTG GAAAGCATGC
    GAGGACGTGC TGACCCAAAT GGGCCTCTTG CCTCTGCTGG ATCACTTACT CGAGACAAAG
    GCGCATGTGA TGCATCCCAA TGTCGAACTG CAGATCCTGG CGCCCCCGAA CAAGCGGGCG
    ACGCGCACCG AGTTTGTGAT CATCTCTAAC AAGAGAGGTA TCATCAGCTC CGGGACGGAG
    TTTCTGGATG AGCCGAGTGC CGTTGACGAT GGGCTGGTGT CGGTGGAGCC ATATGACGAT
    ACTCCGGAAC ATGACGAGGT GTTTTCTTGC AAGCTCTTTG TTGGAGGGAA GCAGGTGGCT
    GACGTGACGG GAGCGGCGAC GAAGGAGGAG GCCAGGGTAA GGGCGGCGGA GAAGGGGTGC
    TTGGTGATCA AGGCGGAAAG GAAGGTATGG AACGAAGCGA AAGCTGCTGC TAAAGAGGAT
    AAGGGGCATA ACACGGAGAA TGGAGACGCC AACGCGGATA ATGGGCAGAG CGGGGAAAAG
    GAAGAGGTCC CTGATTGCCG TGATGCGGAT GGGGATACAG TCATGAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B