NCU05267 cDNA ORF clone, Neurospora crassa OR74A

The following NCU05267 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU05267 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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ONf03437 XM_956664.3
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Neurospora crassa OR74A nonsense-mediated mRNA decay factor (NCU05267), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf03437
    Clone ID Related Accession (Same CDS sequence) XM_956664.3
    Accession Version XM_956664.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3423bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product nonsense-mediated mRNA decay factor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). On Feb 23, 2015 this sequence version replaced XM_956664.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGATCGCC AAAGACGGCG TGAGCTGCGG GCGCTGAACT CGAGGGCTTG GAACGGCGAG 
    AAAGATCTCT TTCCCGTCAG CAAGTCCCTC GACTCTACCC TAAAGAAAAA CACTGCTTTT
    ATCAAGCGTC TCCGAACCAC CATCTCCGCC GCCACCCAGA GCACCTTCCT CCAGGAGATC
    CGCACCGTCA GCCTCTCCAA ATACCTTTCC GAAATCATCT CAGCATGCTA CGAGGGCCTC
    TGCAAGCTCA AGTCTCCCGG AGACATCGAG GCCGGCGTCG AGATCGTCAG CGCCCTGCAC
    CAGCGATTCG GACCCGCCGA CTTTACCGAA CACTTGGGCT GGCTTCTGGG CAAGGGCATG
    GCCACTCCTG ATAAGAGTTT CCTCAAGACC CTTACCCCCG AGGCGAGAGA GAAGGAAGAA
    AAAGACCGCT TGGTTCGACA GCGCGTTCTC CTCCGCGTCG TGACCGAGCT CTGGCTTGTT
    GATGTTCTGC GGACCCTGGA CGATATTGCT CGCCCCGACG ACGCTACGAG ACCGACCACG
    GGAGCCAGAG CCGAGCCGAA GCCGCGGTCT TCTTCCGGCA AGGGAGGCGG TGCCGAGCCC
    TTTCCACTCG AAGTTCTGAA GGATCTCTTG AATCACGATC GAGACCACGC CAACCTCCCC
    TTGCTCGTCA TCTTTGTCAA GGCTTTCAGT TGGGATATCT TGGGCATCAA GGCTGCGGGC
    CAAGATGGTC GTAAAACCGT GGAGGAGGAT GGCGCTACCA AGGGCGCCGA GGAGACCGAA
    GGCGACGGAT CCGAAGACAA GGAGGAAGGT GCCGGCGCGG ATCAGGACGG TCCCTTTGCC
    CCTCCTGAGT TGCAGGAGCG CTTCAAGAAT ATCCTCAAGC GCTACTTTGA GGACGTCAAG
    GGCCACCTTG TTCGGGACCA AAAGGCTATC TACAACCAAT CGCGCAAGAA TGCGGAGGCC
    TATGTCAAGT CGGGAGAGGT CTTTGAGGAT CGCCAGTCAA ACTTTGAGAA GCAGGTCAAG
    GCCCAAGAAC GTCTCGTGGC CAATGCGCAG GTTATTGCCG ATATCATTGG CGCGGAGATG
    CCCGAGTTGA AGGATACTGA TGAGGGCAAC GCCGCGAACG GCACAATCGG CCTGGTCAAG
    GCTGGTGAGT ACCTCCGTGG ACAGGGTGAG GGGCCCGGAA TCTGGGAGGA CGAGGAAGAG
    CGTCGCTTCT ACGAAAACTT GGTTGATCTG AAGGGCAAGG TCCCTGGCAT TCTGCTTGAA
    GAAGTTAAGA AGAAGAAAGC AGATGACGAA CAGGTGGGCA GGAAGATTGA CCCTGTTGAA
    GCCGCGGAAG CTGCCAAGGC GGCCGAGGCT GCTGCCGCCG ATGACCAGTC TATTGCTATT
    GCGAACAAGA CAATTGGCGC TCAGGTTGAC GCCCTGCTTG TGCGCCTTCC TGAGCTCATC
    AGCAAAGACG CCATTGACCA GATGGCTATC GACTTCTGCT TCCTCAATTC GAAGGCGTCT
    CGCAACCGGC TCATCAAGGC CTTGACCGAT GTGCCCAAGG GACGTACCGA TCTCCTCCCG
    AACTGGGCTA GGCTCGTTGC CACGCTTGGT CAATACATGC CCGATATCCC CAAGGGCCTC
    GTTGACTACC TCGACAATGA GTTCCGTAGT CTTCAGCGGC GCAAGGAAAA GGAGTTTCTT
    GGGCAGGTCA GGCTGAGCAA CATTCGCTAC CTGGCTGAGC TGACCAAATT TGGCATTGTT
    CCGGAGCACG TTGTCTTCCA CTGTCTCAAG GTCAGTTTGG ATGACTTTTC GCGGATGAAC
    ATTGAGATCA TTTGCAACCT GCTTGAGAAC TGCGGCCGGT ACCTGCTGCG CAACCCGGAG
    ACTTCTCCGC GCATGGCTTC CTTCTTGGAG ACGCTGCAGC GCAAGAAGTC TGTCCAGCAT
    ATTGGACAGG CCGAGAGGAT GCTCATTGAG AACGCCGTCT ACTATGTTGA TCCGCCGCAG
    CGCCCTGCCA TCGAGGTGAA GGAGAGAACG CCTATGGAGC TCTTCATCCG CAAGTTGATT
    TATGGCGATC TCACCAAGAG GAACTACAGC AAGATCTTGA GGCATATTCG CAGGCTGCAC
    TGGGAAGAGA AGGATGTCGT TGCGATTTTG CACAAGGTCT TCTCCAAGCC CGGAAAAGTC
    AAGTATGGTA ACATCCATCT CTTGGCCATC CTGTTGAGCG CCCTCTACAA GTATCATGGC
    GAGTTCGTTG TCGGAGTCAT CGACCAAGTG ACCGAGTCTA TCTGCCTCGG CCTGGAGTCG
    AATGACTTCA AGTTCAACCA GCGTCGCATC GCTGAGGTCA AGTACCTTGG AGAGCTCTAC
    AACTACAGAA TGCTGGAGCA CCCGGTCATC TTCGACACCA TGTACAAGAT CATGACGTTT
    GGTCACGGCG GCCCGCCCGT TCCAGGCCGC GTCAACCCCT TTGATTTACC CGATGACTTC
    TTCCGCATTC GCCTCATCTC CACCATCCTT GAGACGTGCG GTATGTTCTT CAACAAGGGC
    GCTGCTGGCA AGAAACTCGA TTTCTTCCTC TCCTTCTTCC AGTACTACAT CTACACGAAG
    AATCCGCTGC CCATGGACGT CGAATTCCTC GTCCAGGACA TCTTCGCCCT CACCCGGCCG
    CAGTGGAAGC TTGCCTCCAA CCTGGAGGAG GCGTCCAAGG TGTTCCAGCT GGCCATGGTT
    CAGGATCAAA AGACATCTGG AGTCGAGAGG GTCGTCGAGC CGGAGGACAA CGATAGTGAC
    GTGTCCGACG ATGAAGACGG CATGGTCCCA GATGAAGATG AGGACGACGA GTCGGACGAC
    GATGATGGTG GCGAGGCTGA GGACAACGAG CAGCAGGATA ACACGTCGAG GTACAACAGC
    GAATCTGAAG AGGAGGAAAT TGTCGTTACC AGACAGCAGG AGGAGGTTGA TCCCGAGGAG
    GAGGCTGAGT TTGAGCGCGA GTTTGCCAAG ATGATGGCCG AAAGCCTGGA TTCCCGCAAA
    TTCGAGCGCA AGCAGCAGTT TGACCTGCCG CTTCCCATCA AGCTCAAACC TCGTGAGTCT
    ACGGCTGTTG GCGAATCCTC TGCTGCCAAC GAGGCCGGAC CCTCGGAGGC TCCTCCCTCT
    GGGGAGAAGA TGGCTTTTTC GCTTCTCACG AAGAAGGGTA ATCGCCAGCA GACTCGCACC
    GTTGAACTGC CTTCGGACTC GCACTTTGCT ATTGCTATGA AGAAGAAGCA ACAGGCGGAG
    AAGGAAGAGC AGCAGCGCAT CAAGAACCTT GTCCTGAACT ATGATCTCCG GGAGAACGAT
    GATTATGAGG GTAACGAGAA ACTCAACGCT CATCACCATA ACCGCTCCGA TCTTAGGTTG
    AACAAGGAGC GGGGTGGACA AAGAGTGAGA AAGTTGCAGC TTAGTGATGT TGATTGGACT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_961757.2
    CDS358..3780
    Translation

    Target ORF information:

    RefSeq Version XM_956664.3
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A nonsense-mediated mRNA decay factor (NCU05267), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_956664.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGATCGCC AAAGACGGCG TGAGCTGCGG GCGCTGAACT CGAGGGCTTG GAACGGCGAG 
    AAAGATCTCT TTCCCGTCAG CAAGTCCCTC GACTCTACCC TAAAGAAAAA CACTGCTTTT
    ATCAAGCGTC TCCGAACCAC CATCTCCGCC GCCACCCAGA GCACCTTCCT CCAGGAGATC
    CGCACCGTCA GCCTCTCCAA ATACCTTTCC GAAATCATCT CAGCATGCTA CGAGGGCCTC
    TGCAAGCTCA AGTCTCCCGG AGACATCGAG GCCGGCGTCG AGATCGTCAG CGCCCTGCAC
    CAGCGATTCG GACCCGCCGA CTTTACCGAA CACTTGGGCT GGCTTCTGGG CAAGGGCATG
    GCCACTCCTG ATAAGAGTTT CCTCAAGACC CTTACCCCCG AGGCGAGAGA GAAGGAAGAA
    AAAGACCGCT TGGTTCGACA GCGCGTTCTC CTCCGCGTCG TGACCGAGCT CTGGCTTGTT
    GATGTTCTGC GGACCCTGGA CGATATTGCT CGCCCCGACG ACGCTACGAG ACCGACCACG
    GGAGCCAGAG CCGAGCCGAA GCCGCGGTCT TCTTCCGGCA AGGGAGGCGG TGCCGAGCCC
    TTTCCACTCG AAGTTCTGAA GGATCTCTTG AATCACGATC GAGACCACGC CAACCTCCCC
    TTGCTCGTCA TCTTTGTCAA GGCTTTCAGT TGGGATATCT TGGGCATCAA GGCTGCGGGC
    CAAGATGGTC GTAAAACCGT GGAGGAGGAT GGCGCTACCA AGGGCGCCGA GGAGACCGAA
    GGCGACGGAT CCGAAGACAA GGAGGAAGGT GCCGGCGCGG ATCAGGACGG TCCCTTTGCC
    CCTCCTGAGT TGCAGGAGCG CTTCAAGAAT ATCCTCAAGC GCTACTTTGA GGACGTCAAG
    GGCCACCTTG TTCGGGACCA AAAGGCTATC TACAACCAAT CGCGCAAGAA TGCGGAGGCC
    TATGTCAAGT CGGGAGAGGT CTTTGAGGAT CGCCAGTCAA ACTTTGAGAA GCAGGTCAAG
    GCCCAAGAAC GTCTCGTGGC CAATGCGCAG GTTATTGCCG ATATCATTGG CGCGGAGATG
    CCCGAGTTGA AGGATACTGA TGAGGGCAAC GCCGCGAACG GCACAATCGG CCTGGTCAAG
    GCTGGTGAGT ACCTCCGTGG ACAGGGTGAG GGGCCCGGAA TCTGGGAGGA CGAGGAAGAG
    CGTCGCTTCT ACGAAAACTT GGTTGATCTG AAGGGCAAGG TCCCTGGCAT TCTGCTTGAA
    GAAGTTAAGA AGAAGAAAGC AGATGACGAA CAGGTGGGCA GGAAGATTGA CCCTGTTGAA
    GCCGCGGAAG CTGCCAAGGC GGCCGAGGCT GCTGCCGCCG ATGACCAGTC TATTGCTATT
    GCGAACAAGA CAATTGGCGC TCAGGTTGAC GCCCTGCTTG TGCGCCTTCC TGAGCTCATC
    AGCAAAGACG CCATTGACCA GATGGCTATC GACTTCTGCT TCCTCAATTC GAAGGCGTCT
    CGCAACCGGC TCATCAAGGC CTTGACCGAT GTGCCCAAGG GACGTACCGA TCTCCTCCCG
    AACTGGGCTA GGCTCGTTGC CACGCTTGGT CAATACATGC CCGATATCCC CAAGGGCCTC
    GTTGACTACC TCGACAATGA GTTCCGTAGT CTTCAGCGGC GCAAGGAAAA GGAGTTTCTT
    GGGCAGGTCA GGCTGAGCAA CATTCGCTAC CTGGCTGAGC TGACCAAATT TGGCATTGTT
    CCGGAGCACG TTGTCTTCCA CTGTCTCAAG GTCAGTTTGG ATGACTTTTC GCGGATGAAC
    ATTGAGATCA TTTGCAACCT GCTTGAGAAC TGCGGCCGGT ACCTGCTGCG CAACCCGGAG
    ACTTCTCCGC GCATGGCTTC CTTCTTGGAG ACGCTGCAGC GCAAGAAGTC TGTCCAGCAT
    ATTGGACAGG CCGAGAGGAT GCTCATTGAG AACGCCGTCT ACTATGTTGA TCCGCCGCAG
    CGCCCTGCCA TCGAGGTGAA GGAGAGAACG CCTATGGAGC TCTTCATCCG CAAGTTGATT
    TATGGCGATC TCACCAAGAG GAACTACAGC AAGATCTTGA GGCATATTCG CAGGCTGCAC
    TGGGAAGAGA AGGATGTCGT TGCGATTTTG CACAAGGTCT TCTCCAAGCC CGGAAAAGTC
    AAGTATGGTA ACATCCATCT CTTGGCCATC CTGTTGAGCG CCCTCTACAA GTATCATGGC
    GAGTTCGTTG TCGGAGTCAT CGACCAAGTG ACCGAGTCTA TCTGCCTCGG CCTGGAGTCG
    AATGACTTCA AGTTCAACCA GCGTCGCATC GCTGAGGTCA AGTACCTTGG AGAGCTCTAC
    AACTACAGAA TGCTGGAGCA CCCGGTCATC TTCGACACCA TGTACAAGAT CATGACGTTT
    GGTCACGGCG GCCCGCCCGT TCCAGGCCGC GTCAACCCCT TTGATTTACC CGATGACTTC
    TTCCGCATTC GCCTCATCTC CACCATCCTT GAGACGTGCG GTATGTTCTT CAACAAGGGC
    GCTGCTGGCA AGAAACTCGA TTTCTTCCTC TCCTTCTTCC AGTACTACAT CTACACGAAG
    AATCCGCTGC CCATGGACGT CGAATTCCTC GTCCAGGACA TCTTCGCCCT CACCCGGCCG
    CAGTGGAAGC TTGCCTCCAA CCTGGAGGAG GCGTCCAAGG TGTTCCAGCT GGCCATGGTT
    CAGGATCAAA AGACATCTGG AGTCGAGAGG GTCGTCGAGC CGGAGGACAA CGATAGTGAC
    GTGTCCGACG ATGAAGACGG CATGGTCCCA GATGAAGATG AGGACGACGA GTCGGACGAC
    GATGATGGTG GCGAGGCTGA GGACAACGAG CAGCAGGATA ACACGTCGAG GTACAACAGC
    GAATCTGAAG AGGAGGAAAT TGTCGTTACC AGACAGCAGG AGGAGGTTGA TCCCGAGGAG
    GAGGCTGAGT TTGAGCGCGA GTTTGCCAAG ATGATGGCCG AAAGCCTGGA TTCCCGCAAA
    TTCGAGCGCA AGCAGCAGTT TGACCTGCCG CTTCCCATCA AGCTCAAACC TCGTGAGTCT
    ACGGCTGTTG GCGAATCCTC TGCTGCCAAC GAGGCCGGAC CCTCGGAGGC TCCTCCCTCT
    GGGGAGAAGA TGGCTTTTTC GCTTCTCACG AAGAAGGGTA ATCGCCAGCA GACTCGCACC
    GTTGAACTGC CTTCGGACTC GCACTTTGCT ATTGCTATGA AGAAGAAGCA ACAGGCGGAG
    AAGGAAGAGC AGCAGCGCAT CAAGAACCTT GTCCTGAACT ATGATCTCCG GGAGAACGAT
    GATTATGAGG GTAACGAGAA ACTCAACGCT CATCACCATA ACCGCTCCGA TCTTAGGTTG
    AACAAGGAGC GGGGTGGACA AAGAGTGAGA AAGTTGCAGC TTAGTGATGT TGATTGGACT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B