NCU03819 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU03819
Entrez Gene ID	3877336
Full Name	COPII coat assembly protein sec-16
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011396299.1	XP_011394601.1	COPII coat assembly protein sec-16, variant 2
XM_011396298.1	XP_011394600.1	COPII coat assembly protein sec-16
XM_011396297.1	XP_011394599.1	COPII coat assembly protein sec-16, variant 1

Pathways from BioSystems

Homologies

Neurospora crassa	NCU03819	XP_961208.2
Magnaporthe oryzae (rice blast fungus)	MGG_12141	XP_003719774.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU03819 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU03819 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONf11694	XM_011396297.1 Latest version!	Neurospora crassa OR74A COPII coat assembly protein sec-16, variant 1 (NCU03819), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ONf11694	XM_011396298.1 Latest version!	Neurospora crassa OR74A COPII coat assembly protein sec-16 (NCU03819), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ONf11694	XM_011396299.1 Latest version!	Neurospora crassa OR74A COPII coat assembly protein sec-16, variant 2 (NCU03819), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU03819 ( XM_011396299.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394601.1 Neurospora crassa OR74A COPII coat assembly protein sec-16, variant 2 (NCU03819), mRNA.

Price & Availability↑

CloneID	ONf11694
Clone ID Related Accession (Same CDS sequence)	XM_011396299.1 , XM_011396298.1 , XM_011396297.1
Accession Version	XM_011396299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	COPII coat assembly protein sec-16, variant 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026505).

ATGGCTTCAT GGAACCCGGC ATTCATGCCA GAATCGGCCG CCGACCTCCC GGCCCCCTTG 
CCGACTGATA ACACCGTCGC CGACTCAACA GATCATGCGA ACGGTGTAGC CCAAGATGGC 
TCCGACTTCT GGGGAATTGA TGGAGGAGAA GAGGACGTGC CCGCCCAGAA TGGCGCCTCT 
GACTCCTGGT TTCCCGACTA TGGAACAGGC AGTCATACGA CACTACCCGC GACTGATGCG 
CAAGCTACGA GCCAAATAAC CAGTGAAACT GATACCGACA CCCCCGATAC CGCTACCGAA 
CAGACCGAGA CCGCACATGT AACTACGGAA GATGCTGAAG CTGTCGAGCC AGCTCCCACT 
CAGACGGACG AAGCTCCCGA GCCCATCGTA GAGAACGCGC AAGCTACCGA GGCCGTCCCA 
GGAGACGCTG CCACCGTTGA CCTCGTCCCC GAACATGTGG AGCCTACCGA TGCTACTACT 
ACAGAAAAGG CCGATACAGA CGAGCCATCG GATGTCGCTT CCAAGCACAC GAGTACGATG 
TCCTTCACTC GAACCATTCC CCATGAGCCG AGCTGGAGCG ATGACGGCGA TCCTGAGTGG 
AACCTCGCGC GAGCGGATAC GGATCCCTTC AAGTTCTTGC CATCGAGCGA CCGAACCAAC 
TCTTTTCCTC CTGTTCCTCC CCTAGAACAG CACGAGCAAC AGCAGGAAGA GCAACAAGAA 
AATCAACAGG TACAGCAGGT ACAGCAGCAG CAGCAGCAAC CTCAAACCGA TCCTCATTTG 
GAGCAGTCAG CAATTGTCCA GCCCCAGGTC GCAAATTTCC TTGGTGATGA CGATGAGGTC 
GAGGTACAGG GCGATGATGG GTTCTTCAGC CAGTTGTCTG AGGCACAGAA CGATGGATTT 
GATCAGTTTG GCGCCCAAGA CGCGAACAAT TCTCAGCAGT ACATGGTTGG TGACCTAGGT 
GCTAAGGCCA CTGAGGCGCT TGATGCCCGT TTTGAAGAAG GTGTTCCCCT GATCGCACAT 
GATGGAAACT CGACAGCCGA GCGGGAAGAT ACAAAGGACC TATTCGGAGG AGAGGATGCC 
AATGATGAGG ACGACTTTTT CAGCCAGGTT CAGCAAGGGA GCGTCAACCA AGCTGACTTT 
GAGGCGCAGC CGCTCGAACG CAAGTCGACG ATGCAAGTAT TGGGCGCTAT GGATATGGGC 
TCCGTCCAGC CAAGCTTCCC ACCAGTGGAA GAGCAGCCCG AGGAAGAGGC CGTCGCCACG 
GTAGAGACGC AAACCCACGA CCATCCCGAG ATAACGAGTA CAGATCTGAA CACAGGTGAA 
GGTCACGAGG AGCAGAAGCC AGCAGGCGAA GACGTTGAGG CCAAGTGGAA GGAACTGTTT 
GCTGACGATG AAGAGGACAC CCTATCGCTT CTTGATGATT CCACAGAGGC GTCGAATGAG 
ATCAAGGAGA AGGAGATTGA TCCGGCTGCG TTCTTTGGGA GTGATGATGA GGGCTTTCTC 
GACGATTCGG AAGAGTTCCC AACAATGGAG GAAACAACCC AGCCCAGAGC GCCTAGCGTA 
GCTGGCTCCA ACACCACGAC AACCCCTACC ACCCGGTACA TGCCTCAGAC CCAAACTCCA 
AGCCTGACCG GAGCCCCGAA TCCCTATGCC CCGACTGCGC CACCCCTAAC TTCGGCGCCG 
TCAAATCCTT ACTTTCCTGC CGCACCCAGC GCGGTTGCGC AGACGCCGTC TGTTACGCCC 
TTTGGCGCCC CTGTCTCGGC TCCACCTCCA AGCGGGAGAT TTGGATACGG CGCGGTCCCT 
CCGCCACAGA TTGAGAGGAA AGCCCAAAGC TTCGTCGACA AGAAGGGCGG CTACCAGTCA 
CCGTACGACC TTCCTATGGA GGTAGTCAAG GTTAAGAAGC GCCCAAGTAC GGCAACTCTT 
AACAAGCCTG TGACAGCACC GGCCGCTCCA CCGCCTTCCA AAAGCGCTAC TCACCTGCCT 
CCTCCACCAC CTCCTACCTC TGTTCCACAC TCATCTCAGC CTGGTGTCAA GCCACCACCG 
CCTCGTGGTG AAGCCAAGCC GAAGGAGAAG TTCTTTGAAG AGCTTCCTGT GGTCACAAAG 
GCGCGCCCAG CTTCTCGAAA TACATTGGGC ACCGCATCGC CGGCACAGTC TAGCCCATAC 
GGCCCACCGC CCCCGGCTGG CCCACCTTCT ATCGCCTCGC AGCCACCAGT ACCTGGAATG 
CCGTCTGCCA TGGGATCTGT TCCCAACTCT TCCTTTACCC AGCATGCGGA GGTTCCCAAC 
CTGGTTGCGC CGCCACGTGT CAACCCTTAT GCTTCGATAC AGCCCAGCAC TACGAGCCTT 
GCTCCCGCTG TTCCTGCCCC GGCTTCAAGT CGTTATTCTC CAGCGCCTTC CAGTGGGTCA 
CAGCTCAGCA CTCCCGTCCC ACCTGCTGCG GCAAGCCGTT ACTCTCCCGC CCCGCCAGCT 
TCTCGGCCAA CGAGTTCGGG TTATACTCCG ACTACTTCGG TGCCACCGGT TCTACCACCG 
GTTTTGCCGC ATCAACCCCG TACCTCAAGT CCGCTAGCAC ATTTCGAGTC CAGGCCTCTT 
GTTTCTAGCC ATGCAGAAAG TGGCCTGGCG GAAAAGCGGA GCAGCAGCTC GTTGTACGAC 
TTGCGACTGC AGAGAGTGCC TTCTCTACCT CCGACTCAGG AGGTGGAAGA AGAAGGCCCC 
ATGCAGGGTA TGGTGTCCCC CAACCAAGTC CCGGCATCCC ACCAGACCTC TCCTACGGCG 
TCAAGACATG GCGCTATGCC CCACCGTTCT AGAAGGACAC CGCCTCCGCA GTCTCTAACT 
GTGCAGCCGG TGACGTCCCC TCAGAGGGCG GCATACAGCT ATCAGCCTGC GGCTAGTGCT 
CCATCTCACG AATTCGCGCC ACCACCTCGG TCGCAGACTC AATCCCCTGG TGCCCTCTAC 
GGGAACAGGA ATGTAAAGCC TATTGAACCT ATCCCTCGTC CTTCGTCGGT CACTGACGCG 
ACTTCGCCGC GGGCTGCGTT GTATTCTCCT ATCACCATGC AACCCAGTTC TACTTTCCCC 
CCCATTTCTA CGATTACCTC GCGGCCGCGT GGTAACACTC AGAATTTTAA CCTAGTTCCC 
CCGACAGATG GTAGAGAACA TGATCCCCTA CAGAGATGGC GTGGTGTTCC TCTCATTTCC 
TGGGGCGTAG GTGGAGCTCT GGTAACAATG TTCCCCAAGA ACGTGCCACG ATATGGCATG 
GGCCAAACAG CCCCCATGGT TATTCGTAGC CCAGGAGAAG TCAAGGTCAA GAGCATGAAG 
GACATAGAGC CAATGGAGGA GCGCTTGGCC AAATTCCCTG GGCCTCTAAA GGGGAAGTCG 
AAGAAGAAGG AGACCATTGC CTGGTTAACA AACGGAATTG AGATGCTGGA GAGGACTGTG 
CCACATAACC TGTCTCATCA GCTCAACCCG TCTCACGATG ACAAGCGGAC AACAGAGAGA 
CTCTTGCTTT GGAAGATTCT GAGGGTCTTT GTCGAGCATG ATGGCGTGTT GGAAGGAAAC 
CCGACCGTTA ACCAAGCTGT GCGCGAAATC CTGTACCCGG AGGCTTCAAC CATTGGTGGT 
CAGCCTGAGT TCGCAAATGC TCTGAACCCA TCAGGAATGG GCAATTCTGC CACGACTTCA 
CTACAAGCCG ACTCGGTTGA TTCTTCTGCT GTTGAGCAGA TCCGCAACCA CCTCATCAGT 
GGCGACAATG AGAAGGCAGT GTGGGCTGCG GTCGATAAGC GGCTTTGGGG CCATGCCTTC 
CTGCTGGCCA ACGCCCTTAA CCCTGACCTC TATAAGCGGG TCGCTCAAGA ATTTGTGAAG 
AACGAGGTGA ATTCCACCGG TCATAACAAT GAGTCGCTTG CTGCTCTCTA TGATGTCCTC 
TCTGGCAACC ACGAAGAAAG CGTGGACGAG CTGGTGCCCG CCCATGCTCG AGCTGGTCTT 
CAGCTAGTGG CCAAAAATTC ATCTTCTGGT CCCTCCAAGG ACGCCATGGG TGGTCTTGAT 
AAGTGGCGCG AAACCCTCTC CCTGATCCTC AGCAACAGGA CTGCTGATGA TGCCCGAGCC 
ATCAACTTTT TGGGCAATTT GCTATCAGGC TATGGAAGAG CCGAGGCGGC TCATATTTGC 
TTCTTGTTTG CCAGAAGTCA AACCATCTTC GGCGGCTTGG ACAACCCGAA CTCTAACTTC 
GTTCTTGTTG GGTCTGACCA TAGGCGGCAG GCGGACCATT TCGCAAAGGA GATTGAGCCT 
CTTTTGTTGA GCGAGGTTTA TGAGTACGGC CAGAGCCTTG CTGGTGGTAC CGTGCCCGTC 
AGCAATCCTC ACCTGGCAGC GTACAAGCTT CAGCACGCAT ACGCCCTGGC CGAGTATGGC 
TTTAGAGACA AGGCCCTTCA ATACTGCGAG GCTATCACGG CTGCGATCAC CGCGCAGACG 
AAGCGCTCTC CTTATTACCA TCCCATCCTT GAGGCCTATG TCGATGACTT GATGAAGCGA 
TTGAGGCAGG CACCCAAGGA GGAATCAAAC TCGTGGATCC CGAAGCCTAG CATGAATAAG 
GTATCTGATT CCATGTGGAA TCGGTTCAAC AAGTTTGTCG CTGGAGATGA CAACGAGGAT 
GGCAGTAAGG GCTCACCTGA CGCTGCGGGA GAATCTGGTC CCTTTGCACG AATCGCCGGA 
GGAACCCCGA CTATCAGTCG CTCGCCCTCT GTCAACAATC TTGAGACCTT TGGCGCGACC 
ATCCCCAGCT ATGGCATGCC TAGTGCTCCC GTGACGAACG GTCCTAATAT GTTCTCTCCT 
CCTCCTCCTA CACGGACGGC TTCACGCTAC GCACCAGGCG CTCCTCAACC ATCGACTCCG 
AACTATAACC CCTATGAGAC AAACTCTCCA TATGCGCCTC GCTCATCAAT GGAACGGGCC 
TCAGGCGAGT ACAGCAGAAG TTCAGTTGAG CTTCCGCGTC AGTCTTTGGA CTCTCAGCGG 
GGCTATTCGC ACAGCAGCTA TGCTCCCAAC CGTACATCTT CGCCTGCTCA GCCATATACA 
CCTTACGGCA CGACGCCACA GGAATCATCC TACTCTCTGC ATAACATGCA GCCTCAGCAA 
AGTTTGACAT CCCCAGCGGC TACTACTTCT GGTTATCAAC CCTTCACTCC ACAAAACAAC 
GTCTCGGCAA ATGATGAGCC CTCTAACGAA CCCCCTTCGG CCCCGTCAAC TGGCTACCAG 
CCGCCGTCGT ACGGATATGA GCCTCCGTCA TTCACCCCTT ACGAGGCGCC GGCGACCAAT 
GACGAGGAGG AGGGTGCCTC CAAGGAGAAC GGTGACTCTA ACCAGGGTGG CGAAGGTGTC 
AACACCTTTG AGCCCCCTTC ATTCCAGCCA TATAGCTATG AACCGCCTTC TTACGAACCC 
GACACCCCAC CTTCGAAAGA TGACGATCAA TCTGAGGAAG AAAAGCCCAA GCCGAAGAAG 
AAGGGCCCTA TGTACGATGA CGATGACGAT GATTTCCCCG CGGCGCCGAA GCCTGCCGGC 
AAGTCCAAGG CTGAAATTGA CCGGGAGAAC GAGGAAATGG TCCGTCGGAT CGCCGAAGAA 
GAAGCCAAAC GCGCCGCAGA AGCCAAAGCC GCCAAGAAGG GCTGGGGCTT CACCAGCTGG 
TTCGCAAAGA AGGAGGCTGC CGCCGCCGAT GCCAACGCCG CCGGAAGCAG CCCCGGCAAG 
CCCATCCGTG CCAAGCTCGG CGAGGCCAAC TCGTTCTACT ACGACCCCGA GCAAAAGCGC 
TGGATCAACA AGAACGCCAG TCCCGAAGAC CAAGCCGCCA AGAAATCCAC GCCACCACCA 
CCCAAGGGCG GCATCCCCCG CTCATCCGCC TCCAGCCCTG CTCCTCCCAT GGGTATGGGC 
GTGGGCGGCG GTAGCGCGCC CAACACCCCC GGCCGCGCCA GCGCCCCGCC TACTGGTCCG 
CCTAGACCGG CGGCGCTCAT GCCTTCGGCG TCGGAGAGCA ATGTTGGGTC TGGCCCACCT 
AGCGCTGTTG GTCCGCTGAG TCCCAGTGGA AGTAACGGGC CCCCAAGTGC GGGCTTGCTT 
TCCCCTGGCA TGGGTCCGGC GGCGATGCAG CGCCCGGCCT CGACGAGCAC GAGCGGGCCC 
CCTGCTGGTA CTAGTAAGCC GTTGAGCGCG ACGAGTAGTA TTGATGATTT GTTGGGAGCG 
GCGGTACCGA GGAAGAGGGG TGAGGCGAAG AAGCCGAGGA AGGCGGCTAG GTATGTGGAT 
GTTATGCAGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394601.1
CDS	167..6421
Translation	MASWNPAFMPESAADLPAPLPTDNTVADSTDHANGVAQDGSDFWGIDGGEEDVPAQNGASDSWFPDYGTGSHTTLPATDAQATSQITSETDTDTPDTATEQTETAHVTTEDAEAVEPAPTQTDEAPEPIVENAQATEAVPGDAATVDLVPEHVEPTDATTTEKADTDEPSDVASKHTSTMSFTRTIPHEPSWSDDGDPEWNLARADTDPFKFLPSSDRTNSFPPVPPLEQHEQQQEEQQENQQVQQVQQQQQQPQTDPHLEQSAIVQPQVANFLGDDDEVEVQGDDGFFSQLSEAQNDGFDQFGAQDANNSQQYMVGDLGAKATEALDARFEEGVPLIAHDGNSTAEREDTKDLFGGEDANDEDDFFSQVQQGSVNQADFEAQPLERKSTMQVLGAMDMGSVQPSFPPVEEQPEEEAVATVETQTHDHPEITSTDLNTGEGHEEQKPAGEDVEAKWKELFADDEEDTLSLLDDSTEASNEIKEKEIDPAAFFGSDDEGFLDDSEEFPTMEETTQPRAPSVAGSNTTTTPTTRYMPQTQTPSLTGAPNPYAPTAPPLTSAPSNPYFPAAPSAVAQTPSVTPFGAPVSAPPPSGRFGYGAVPPPQIERKAQSFVDKKGGYQSPYDLPMEVVKVKKRPSTATLNKPVTAPAAPPPSKSATHLPPPPPPTSVPHSSQPGVKPPPPRGEAKPKEKFFEELPVVTKARPASRNTLGTASPAQSSPYGPPPPAGPPSIASQPPVPGMPSAMGSVPNSSFTQHAEVPNLVAPPRVNPYASIQPSTTSLAPAVPAPASSRYSPAPSSGSQLSTPVPPAAASRYSPAPPASRPTSSGYTPTTSVPPVLPPVLPHQPRTSSPLAHFESRPLVSSHAESGLAEKRSSSSLYDLRLQRVPSLPPTQEVEEEGPMQGMVSPNQVPASHQTSPTASRHGAMPHRSRRTPPPQSLTVQPVTSPQRAAYSYQPAASAPSHEFAPPPRSQTQSPGALYGNRNVKPIEPIPRPSSVTDATSPRAALYSPITMQPSSTFPPISTITSRPRGNTQNFNLVPPTDGREHDPLQRWRGVPLISWGVGGALVTMFPKNVPRYGMGQTAPMVIRSPGEVKVKSMKDIEPMEERLAKFPGPLKGKSKKKETIAWLTNGIEMLERTVPHNLSHQLNPSHDDKRTTERLLLWKILRVFVEHDGVLEGNPTVNQAVREILYPEASTIGGQPEFANALNPSGMGNSATTSLQADSVDSSAVEQIRNHLISGDNEKAVWAAVDKRLWGHAFLLANALNPDLYKRVAQEFVKNEVNSTGHNNESLAALYDVLSGNHEESVDELVPAHARAGLQLVAKNSSSGPSKDAMGGLDKWRETLSLILSNRTADDARAINFLGNLLSGYGRAEAAHICFLFARSQTIFGGLDNPNSNFVLVGSDHRRQADHFAKEIEPLLLSEVYEYGQSLAGGTVPVSNPHLAAYKLQHAYALAEYGFRDKALQYCEAITAAITAQTKRSPYYHPILEAYVDDLMKRLRQAPKEESNSWIPKPSMNKVSDSMWNRFNKFVAGDDNEDGSKGSPDAAGESGPFARIAGGTPTISRSPSVNNLETFGATIPSYGMPSAPVTNGPNMFSPPPPTRTASRYAPGAPQPSTPNYNPYETNSPYAPRSSMERASGEYSRSSVELPRQSLDSQRGYSHSSYAPNRTSSPAQPYTPYGTTPQESSYSLHNMQPQQSLTSPAATTSGYQPFTPQNNVSANDEPSNEPPSAPSTGYQPPSYGYEPPSFTPYEAPATNDEEEGASKENGDSNQGGEGVNTFEPPSFQPYSYEPPSYEPDTPPSKDDDQSEEEKPKPKKKGPMYDDDDDDFPAAPKPAGKSKAEIDRENEEMVRRIAEEEAKRAAEAKAAKKGWGFTSWFAKKEAAAADANAAGSSPGKPIRAKLGEANSFYYDPEQKRWINKNASPEDQAAKKSTPPPPKGGIPRSSASSPAPPMGMGVGGGSAPNTPGRASAPPTGPPRPAALMPSASESNVGSGPPSAVGPLSPSGSNGPPSAGLLSPGMGPAAMQRPASTSTSGPPAGTSKPLSATSSIDDLLGAAVPRKRGEAKKPRKAARYVDVMQK

Target ORF information:

RefSeq Version	XM_011396299.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A COPII coat assembly protein sec-16, variant 2 (NCU03819), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396299.1

ORF Insert Sequence:

ATGGCTTCAT GGAACCCGGC ATTCATGCCA GAATCGGCCG CCGACCTCCC GGCCCCCTTG 
CCGACTGATA ACACCGTCGC CGACTCAACA GATCATGCGA ACGGTGTAGC CCAAGATGGC 
TCCGACTTCT GGGGAATTGA TGGAGGAGAA GAGGACGTGC CCGCCCAGAA TGGCGCCTCT 
GACTCCTGGT TTCCCGACTA TGGAACAGGC AGTCATACGA CACTACCCGC GACTGATGCG 
CAAGCTACGA GCCAAATAAC CAGTGAAACT GATACCGACA CCCCCGATAC CGCTACCGAA 
CAGACCGAGA CCGCACATGT AACTACGGAA GATGCTGAAG CTGTCGAGCC AGCTCCCACT 
CAGACGGACG AAGCTCCCGA GCCCATCGTA GAGAACGCGC AAGCTACCGA GGCCGTCCCA 
GGAGACGCTG CCACCGTTGA CCTCGTCCCC GAACATGTGG AGCCTACCGA TGCTACTACT 
ACAGAAAAGG CCGATACAGA CGAGCCATCG GATGTCGCTT CCAAGCACAC GAGTACGATG 
TCCTTCACTC GAACCATTCC CCATGAGCCG AGCTGGAGCG ATGACGGCGA TCCTGAGTGG 
AACCTCGCGC GAGCGGATAC GGATCCCTTC AAGTTCTTGC CATCGAGCGA CCGAACCAAC 
TCTTTTCCTC CTGTTCCTCC CCTAGAACAG CACGAGCAAC AGCAGGAAGA GCAACAAGAA 
AATCAACAGG TACAGCAGGT ACAGCAGCAG CAGCAGCAAC CTCAAACCGA TCCTCATTTG 
GAGCAGTCAG CAATTGTCCA GCCCCAGGTC GCAAATTTCC TTGGTGATGA CGATGAGGTC 
GAGGTACAGG GCGATGATGG GTTCTTCAGC CAGTTGTCTG AGGCACAGAA CGATGGATTT 
GATCAGTTTG GCGCCCAAGA CGCGAACAAT TCTCAGCAGT ACATGGTTGG TGACCTAGGT 
GCTAAGGCCA CTGAGGCGCT TGATGCCCGT TTTGAAGAAG GTGTTCCCCT GATCGCACAT 
GATGGAAACT CGACAGCCGA GCGGGAAGAT ACAAAGGACC TATTCGGAGG AGAGGATGCC 
AATGATGAGG ACGACTTTTT CAGCCAGGTT CAGCAAGGGA GCGTCAACCA AGCTGACTTT 
GAGGCGCAGC CGCTCGAACG CAAGTCGACG ATGCAAGTAT TGGGCGCTAT GGATATGGGC 
TCCGTCCAGC CAAGCTTCCC ACCAGTGGAA GAGCAGCCCG AGGAAGAGGC CGTCGCCACG 
GTAGAGACGC AAACCCACGA CCATCCCGAG ATAACGAGTA CAGATCTGAA CACAGGTGAA 
GGTCACGAGG AGCAGAAGCC AGCAGGCGAA GACGTTGAGG CCAAGTGGAA GGAACTGTTT 
GCTGACGATG AAGAGGACAC CCTATCGCTT CTTGATGATT CCACAGAGGC GTCGAATGAG 
ATCAAGGAGA AGGAGATTGA TCCGGCTGCG TTCTTTGGGA GTGATGATGA GGGCTTTCTC 
GACGATTCGG AAGAGTTCCC AACAATGGAG GAAACAACCC AGCCCAGAGC GCCTAGCGTA 
GCTGGCTCCA ACACCACGAC AACCCCTACC ACCCGGTACA TGCCTCAGAC CCAAACTCCA 
AGCCTGACCG GAGCCCCGAA TCCCTATGCC CCGACTGCGC CACCCCTAAC TTCGGCGCCG 
TCAAATCCTT ACTTTCCTGC CGCACCCAGC GCGGTTGCGC AGACGCCGTC TGTTACGCCC 
TTTGGCGCCC CTGTCTCGGC TCCACCTCCA AGCGGGAGAT TTGGATACGG CGCGGTCCCT 
CCGCCACAGA TTGAGAGGAA AGCCCAAAGC TTCGTCGACA AGAAGGGCGG CTACCAGTCA 
CCGTACGACC TTCCTATGGA GGTAGTCAAG GTTAAGAAGC GCCCAAGTAC GGCAACTCTT 
AACAAGCCTG TGACAGCACC GGCCGCTCCA CCGCCTTCCA AAAGCGCTAC TCACCTGCCT 
CCTCCACCAC CTCCTACCTC TGTTCCACAC TCATCTCAGC CTGGTGTCAA GCCACCACCG 
CCTCGTGGTG AAGCCAAGCC GAAGGAGAAG TTCTTTGAAG AGCTTCCTGT GGTCACAAAG 
GCGCGCCCAG CTTCTCGAAA TACATTGGGC ACCGCATCGC CGGCACAGTC TAGCCCATAC 
GGCCCACCGC CCCCGGCTGG CCCACCTTCT ATCGCCTCGC AGCCACCAGT ACCTGGAATG 
CCGTCTGCCA TGGGATCTGT TCCCAACTCT TCCTTTACCC AGCATGCGGA GGTTCCCAAC 
CTGGTTGCGC CGCCACGTGT CAACCCTTAT GCTTCGATAC AGCCCAGCAC TACGAGCCTT 
GCTCCCGCTG TTCCTGCCCC GGCTTCAAGT CGTTATTCTC CAGCGCCTTC CAGTGGGTCA 
CAGCTCAGCA CTCCCGTCCC ACCTGCTGCG GCAAGCCGTT ACTCTCCCGC CCCGCCAGCT 
TCTCGGCCAA CGAGTTCGGG TTATACTCCG ACTACTTCGG TGCCACCGGT TCTACCACCG 
GTTTTGCCGC ATCAACCCCG TACCTCAAGT CCGCTAGCAC ATTTCGAGTC CAGGCCTCTT 
GTTTCTAGCC ATGCAGAAAG TGGCCTGGCG GAAAAGCGGA GCAGCAGCTC GTTGTACGAC 
TTGCGACTGC AGAGAGTGCC TTCTCTACCT CCGACTCAGG AGGTGGAAGA AGAAGGCCCC 
ATGCAGGGTA TGGTGTCCCC CAACCAAGTC CCGGCATCCC ACCAGACCTC TCCTACGGCG 
TCAAGACATG GCGCTATGCC CCACCGTTCT AGAAGGACAC CGCCTCCGCA GTCTCTAACT 
GTGCAGCCGG TGACGTCCCC TCAGAGGGCG GCATACAGCT ATCAGCCTGC GGCTAGTGCT 
CCATCTCACG AATTCGCGCC ACCACCTCGG TCGCAGACTC AATCCCCTGG TGCCCTCTAC 
GGGAACAGGA ATGTAAAGCC TATTGAACCT ATCCCTCGTC CTTCGTCGGT CACTGACGCG 
ACTTCGCCGC GGGCTGCGTT GTATTCTCCT ATCACCATGC AACCCAGTTC TACTTTCCCC 
CCCATTTCTA CGATTACCTC GCGGCCGCGT GGTAACACTC AGAATTTTAA CCTAGTTCCC 
CCGACAGATG GTAGAGAACA TGATCCCCTA CAGAGATGGC GTGGTGTTCC TCTCATTTCC 
TGGGGCGTAG GTGGAGCTCT GGTAACAATG TTCCCCAAGA ACGTGCCACG ATATGGCATG 
GGCCAAACAG CCCCCATGGT TATTCGTAGC CCAGGAGAAG TCAAGGTCAA GAGCATGAAG 
GACATAGAGC CAATGGAGGA GCGCTTGGCC AAATTCCCTG GGCCTCTAAA GGGGAAGTCG 
AAGAAGAAGG AGACCATTGC CTGGTTAACA AACGGAATTG AGATGCTGGA GAGGACTGTG 
CCACATAACC TGTCTCATCA GCTCAACCCG TCTCACGATG ACAAGCGGAC AACAGAGAGA 
CTCTTGCTTT GGAAGATTCT GAGGGTCTTT GTCGAGCATG ATGGCGTGTT GGAAGGAAAC 
CCGACCGTTA ACCAAGCTGT GCGCGAAATC CTGTACCCGG AGGCTTCAAC CATTGGTGGT 
CAGCCTGAGT TCGCAAATGC TCTGAACCCA TCAGGAATGG GCAATTCTGC CACGACTTCA 
CTACAAGCCG ACTCGGTTGA TTCTTCTGCT GTTGAGCAGA TCCGCAACCA CCTCATCAGT 
GGCGACAATG AGAAGGCAGT GTGGGCTGCG GTCGATAAGC GGCTTTGGGG CCATGCCTTC 
CTGCTGGCCA ACGCCCTTAA CCCTGACCTC TATAAGCGGG TCGCTCAAGA ATTTGTGAAG 
AACGAGGTGA ATTCCACCGG TCATAACAAT GAGTCGCTTG CTGCTCTCTA TGATGTCCTC 
TCTGGCAACC ACGAAGAAAG CGTGGACGAG CTGGTGCCCG CCCATGCTCG AGCTGGTCTT 
CAGCTAGTGG CCAAAAATTC ATCTTCTGGT CCCTCCAAGG ACGCCATGGG TGGTCTTGAT 
AAGTGGCGCG AAACCCTCTC CCTGATCCTC AGCAACAGGA CTGCTGATGA TGCCCGAGCC 
ATCAACTTTT TGGGCAATTT GCTATCAGGC TATGGAAGAG CCGAGGCGGC TCATATTTGC 
TTCTTGTTTG CCAGAAGTCA AACCATCTTC GGCGGCTTGG ACAACCCGAA CTCTAACTTC 
GTTCTTGTTG GGTCTGACCA TAGGCGGCAG GCGGACCATT TCGCAAAGGA GATTGAGCCT 
CTTTTGTTGA GCGAGGTTTA TGAGTACGGC CAGAGCCTTG CTGGTGGTAC CGTGCCCGTC 
AGCAATCCTC ACCTGGCAGC GTACAAGCTT CAGCACGCAT ACGCCCTGGC CGAGTATGGC 
TTTAGAGACA AGGCCCTTCA ATACTGCGAG GCTATCACGG CTGCGATCAC CGCGCAGACG 
AAGCGCTCTC CTTATTACCA TCCCATCCTT GAGGCCTATG TCGATGACTT GATGAAGCGA 
TTGAGGCAGG CACCCAAGGA GGAATCAAAC TCGTGGATCC CGAAGCCTAG CATGAATAAG 
GTATCTGATT CCATGTGGAA TCGGTTCAAC AAGTTTGTCG CTGGAGATGA CAACGAGGAT 
GGCAGTAAGG GCTCACCTGA CGCTGCGGGA GAATCTGGTC CCTTTGCACG AATCGCCGGA 
GGAACCCCGA CTATCAGTCG CTCGCCCTCT GTCAACAATC TTGAGACCTT TGGCGCGACC 
ATCCCCAGCT ATGGCATGCC TAGTGCTCCC GTGACGAACG GTCCTAATAT GTTCTCTCCT 
CCTCCTCCTA CACGGACGGC TTCACGCTAC GCACCAGGCG CTCCTCAACC ATCGACTCCG 
AACTATAACC CCTATGAGAC AAACTCTCCA TATGCGCCTC GCTCATCAAT GGAACGGGCC 
TCAGGCGAGT ACAGCAGAAG TTCAGTTGAG CTTCCGCGTC AGTCTTTGGA CTCTCAGCGG 
GGCTATTCGC ACAGCAGCTA TGCTCCCAAC CGTACATCTT CGCCTGCTCA GCCATATACA 
CCTTACGGCA CGACGCCACA GGAATCATCC TACTCTCTGC ATAACATGCA GCCTCAGCAA 
AGTTTGACAT CCCCAGCGGC TACTACTTCT GGTTATCAAC CCTTCACTCC ACAAAACAAC 
GTCTCGGCAA ATGATGAGCC CTCTAACGAA CCCCCTTCGG CCCCGTCAAC TGGCTACCAG 
CCGCCGTCGT ACGGATATGA GCCTCCGTCA TTCACCCCTT ACGAGGCGCC GGCGACCAAT 
GACGAGGAGG AGGGTGCCTC CAAGGAGAAC GGTGACTCTA ACCAGGGTGG CGAAGGTGTC 
AACACCTTTG AGCCCCCTTC ATTCCAGCCA TATAGCTATG AACCGCCTTC TTACGAACCC 
GACACCCCAC CTTCGAAAGA TGACGATCAA TCTGAGGAAG AAAAGCCCAA GCCGAAGAAG 
AAGGGCCCTA TGTACGATGA CGATGACGAT GATTTCCCCG CGGCGCCGAA GCCTGCCGGC 
AAGTCCAAGG CTGAAATTGA CCGGGAGAAC GAGGAAATGG TCCGTCGGAT CGCCGAAGAA 
GAAGCCAAAC GCGCCGCAGA AGCCAAAGCC GCCAAGAAGG GCTGGGGCTT CACCAGCTGG 
TTCGCAAAGA AGGAGGCTGC CGCCGCCGAT GCCAACGCCG CCGGAAGCAG CCCCGGCAAG 
CCCATCCGTG CCAAGCTCGG CGAGGCCAAC TCGTTCTACT ACGACCCCGA GCAAAAGCGC 
TGGATCAACA AGAACGCCAG TCCCGAAGAC CAAGCCGCCA AGAAATCCAC GCCACCACCA 
CCCAAGGGCG GCATCCCCCG CTCATCCGCC TCCAGCCCTG CTCCTCCCAT GGGTATGGGC 
GTGGGCGGCG GTAGCGCGCC CAACACCCCC GGCCGCGCCA GCGCCCCGCC TACTGGTCCG 
CCTAGACCGG CGGCGCTCAT GCCTTCGGCG TCGGAGAGCA ATGTTGGGTC TGGCCCACCT 
AGCGCTGTTG GTCCGCTGAG TCCCAGTGGA AGTAACGGGC CCCCAAGTGC GGGCTTGCTT 
TCCCCTGGCA TGGGTCCGGC GGCGATGCAG CGCCCGGCCT CGACGAGCAC GAGCGGGCCC 
CCTGCTGGTA CTAGTAAGCC GTTGAGCGCG ACGAGTAGTA TTGATGATTT GTTGGGAGCG 
GCGGTACCGA GGAAGAGGGG TGAGGCGAAG AAGCCGAGGA AGGCGGCTAG GTATGTGGAT 
GTTATGCAGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU03819 ( XM_011396298.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394600.1 Neurospora crassa OR74A COPII coat assembly protein sec-16 (NCU03819), mRNA.

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CloneID	ONf11694
Clone ID Related Accession (Same CDS sequence)	XM_011396299.1 , XM_011396298.1 , XM_011396297.1
Accession Version	XM_011396298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	COPII coat assembly protein sec-16
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026505).

ATGGCTTCAT GGAACCCGGC ATTCATGCCA GAATCGGCCG CCGACCTCCC GGCCCCCTTG 
CCGACTGATA ACACCGTCGC CGACTCAACA GATCATGCGA ACGGTGTAGC CCAAGATGGC 
TCCGACTTCT GGGGAATTGA TGGAGGAGAA GAGGACGTGC CCGCCCAGAA TGGCGCCTCT 
GACTCCTGGT TTCCCGACTA TGGAACAGGC AGTCATACGA CACTACCCGC GACTGATGCG 
CAAGCTACGA GCCAAATAAC CAGTGAAACT GATACCGACA CCCCCGATAC CGCTACCGAA 
CAGACCGAGA CCGCACATGT AACTACGGAA GATGCTGAAG CTGTCGAGCC AGCTCCCACT 
CAGACGGACG AAGCTCCCGA GCCCATCGTA GAGAACGCGC AAGCTACCGA GGCCGTCCCA 
GGAGACGCTG CCACCGTTGA CCTCGTCCCC GAACATGTGG AGCCTACCGA TGCTACTACT 
ACAGAAAAGG CCGATACAGA CGAGCCATCG GATGTCGCTT CCAAGCACAC GAGTACGATG 
TCCTTCACTC GAACCATTCC CCATGAGCCG AGCTGGAGCG ATGACGGCGA TCCTGAGTGG 
AACCTCGCGC GAGCGGATAC GGATCCCTTC AAGTTCTTGC CATCGAGCGA CCGAACCAAC 
TCTTTTCCTC CTGTTCCTCC CCTAGAACAG CACGAGCAAC AGCAGGAAGA GCAACAAGAA 
AATCAACAGG TACAGCAGGT ACAGCAGCAG CAGCAGCAAC CTCAAACCGA TCCTCATTTG 
GAGCAGTCAG CAATTGTCCA GCCCCAGGTC GCAAATTTCC TTGGTGATGA CGATGAGGTC 
GAGGTACAGG GCGATGATGG GTTCTTCAGC CAGTTGTCTG AGGCACAGAA CGATGGATTT 
GATCAGTTTG GCGCCCAAGA CGCGAACAAT TCTCAGCAGT ACATGGTTGG TGACCTAGGT 
GCTAAGGCCA CTGAGGCGCT TGATGCCCGT TTTGAAGAAG GTGTTCCCCT GATCGCACAT 
GATGGAAACT CGACAGCCGA GCGGGAAGAT ACAAAGGACC TATTCGGAGG AGAGGATGCC 
AATGATGAGG ACGACTTTTT CAGCCAGGTT CAGCAAGGGA GCGTCAACCA AGCTGACTTT 
GAGGCGCAGC CGCTCGAACG CAAGTCGACG ATGCAAGTAT TGGGCGCTAT GGATATGGGC 
TCCGTCCAGC CAAGCTTCCC ACCAGTGGAA GAGCAGCCCG AGGAAGAGGC CGTCGCCACG 
GTAGAGACGC AAACCCACGA CCATCCCGAG ATAACGAGTA CAGATCTGAA CACAGGTGAA 
GGTCACGAGG AGCAGAAGCC AGCAGGCGAA GACGTTGAGG CCAAGTGGAA GGAACTGTTT 
GCTGACGATG AAGAGGACAC CCTATCGCTT CTTGATGATT CCACAGAGGC GTCGAATGAG 
ATCAAGGAGA AGGAGATTGA TCCGGCTGCG TTCTTTGGGA GTGATGATGA GGGCTTTCTC 
GACGATTCGG AAGAGTTCCC AACAATGGAG GAAACAACCC AGCCCAGAGC GCCTAGCGTA 
GCTGGCTCCA ACACCACGAC AACCCCTACC ACCCGGTACA TGCCTCAGAC CCAAACTCCA 
AGCCTGACCG GAGCCCCGAA TCCCTATGCC CCGACTGCGC CACCCCTAAC TTCGGCGCCG 
TCAAATCCTT ACTTTCCTGC CGCACCCAGC GCGGTTGCGC AGACGCCGTC TGTTACGCCC 
TTTGGCGCCC CTGTCTCGGC TCCACCTCCA AGCGGGAGAT TTGGATACGG CGCGGTCCCT 
CCGCCACAGA TTGAGAGGAA AGCCCAAAGC TTCGTCGACA AGAAGGGCGG CTACCAGTCA 
CCGTACGACC TTCCTATGGA GGTAGTCAAG GTTAAGAAGC GCCCAAGTAC GGCAACTCTT 
AACAAGCCTG TGACAGCACC GGCCGCTCCA CCGCCTTCCA AAAGCGCTAC TCACCTGCCT 
CCTCCACCAC CTCCTACCTC TGTTCCACAC TCATCTCAGC CTGGTGTCAA GCCACCACCG 
CCTCGTGGTG AAGCCAAGCC GAAGGAGAAG TTCTTTGAAG AGCTTCCTGT GGTCACAAAG 
GCGCGCCCAG CTTCTCGAAA TACATTGGGC ACCGCATCGC CGGCACAGTC TAGCCCATAC 
GGCCCACCGC CCCCGGCTGG CCCACCTTCT ATCGCCTCGC AGCCACCAGT ACCTGGAATG 
CCGTCTGCCA TGGGATCTGT TCCCAACTCT TCCTTTACCC AGCATGCGGA GGTTCCCAAC 
CTGGTTGCGC CGCCACGTGT CAACCCTTAT GCTTCGATAC AGCCCAGCAC TACGAGCCTT 
GCTCCCGCTG TTCCTGCCCC GGCTTCAAGT CGTTATTCTC CAGCGCCTTC CAGTGGGTCA 
CAGCTCAGCA CTCCCGTCCC ACCTGCTGCG GCAAGCCGTT ACTCTCCCGC CCCGCCAGCT 
TCTCGGCCAA CGAGTTCGGG TTATACTCCG ACTACTTCGG TGCCACCGGT TCTACCACCG 
GTTTTGCCGC ATCAACCCCG TACCTCAAGT CCGCTAGCAC ATTTCGAGTC CAGGCCTCTT 
GTTTCTAGCC ATGCAGAAAG TGGCCTGGCG GAAAAGCGGA GCAGCAGCTC GTTGTACGAC 
TTGCGACTGC AGAGAGTGCC TTCTCTACCT CCGACTCAGG AGGTGGAAGA AGAAGGCCCC 
ATGCAGGGTA TGGTGTCCCC CAACCAAGTC CCGGCATCCC ACCAGACCTC TCCTACGGCG 
TCAAGACATG GCGCTATGCC CCACCGTTCT AGAAGGACAC CGCCTCCGCA GTCTCTAACT 
GTGCAGCCGG TGACGTCCCC TCAGAGGGCG GCATACAGCT ATCAGCCTGC GGCTAGTGCT 
CCATCTCACG AATTCGCGCC ACCACCTCGG TCGCAGACTC AATCCCCTGG TGCCCTCTAC 
GGGAACAGGA ATGTAAAGCC TATTGAACCT ATCCCTCGTC CTTCGTCGGT CACTGACGCG 
ACTTCGCCGC GGGCTGCGTT GTATTCTCCT ATCACCATGC AACCCAGTTC TACTTTCCCC 
CCCATTTCTA CGATTACCTC GCGGCCGCGT GGTAACACTC AGAATTTTAA CCTAGTTCCC 
CCGACAGATG GTAGAGAACA TGATCCCCTA CAGAGATGGC GTGGTGTTCC TCTCATTTCC 
TGGGGCGTAG GTGGAGCTCT GGTAACAATG TTCCCCAAGA ACGTGCCACG ATATGGCATG 
GGCCAAACAG CCCCCATGGT TATTCGTAGC CCAGGAGAAG TCAAGGTCAA GAGCATGAAG 
GACATAGAGC CAATGGAGGA GCGCTTGGCC AAATTCCCTG GGCCTCTAAA GGGGAAGTCG 
AAGAAGAAGG AGACCATTGC CTGGTTAACA AACGGAATTG AGATGCTGGA GAGGACTGTG 
CCACATAACC TGTCTCATCA GCTCAACCCG TCTCACGATG ACAAGCGGAC AACAGAGAGA 
CTCTTGCTTT GGAAGATTCT GAGGGTCTTT GTCGAGCATG ATGGCGTGTT GGAAGGAAAC 
CCGACCGTTA ACCAAGCTGT GCGCGAAATC CTGTACCCGG AGGCTTCAAC CATTGGTGGT 
CAGCCTGAGT TCGCAAATGC TCTGAACCCA TCAGGAATGG GCAATTCTGC CACGACTTCA 
CTACAAGCCG ACTCGGTTGA TTCTTCTGCT GTTGAGCAGA TCCGCAACCA CCTCATCAGT 
GGCGACAATG AGAAGGCAGT GTGGGCTGCG GTCGATAAGC GGCTTTGGGG CCATGCCTTC 
CTGCTGGCCA ACGCCCTTAA CCCTGACCTC TATAAGCGGG TCGCTCAAGA ATTTGTGAAG 
AACGAGGTGA ATTCCACCGG TCATAACAAT GAGTCGCTTG CTGCTCTCTA TGATGTCCTC 
TCTGGCAACC ACGAAGAAAG CGTGGACGAG CTGGTGCCCG CCCATGCTCG AGCTGGTCTT 
CAGCTAGTGG CCAAAAATTC ATCTTCTGGT CCCTCCAAGG ACGCCATGGG TGGTCTTGAT 
AAGTGGCGCG AAACCCTCTC CCTGATCCTC AGCAACAGGA CTGCTGATGA TGCCCGAGCC 
ATCAACTTTT TGGGCAATTT GCTATCAGGC TATGGAAGAG CCGAGGCGGC TCATATTTGC 
TTCTTGTTTG CCAGAAGTCA AACCATCTTC GGCGGCTTGG ACAACCCGAA CTCTAACTTC 
GTTCTTGTTG GGTCTGACCA TAGGCGGCAG GCGGACCATT TCGCAAAGGA GATTGAGCCT 
CTTTTGTTGA GCGAGGTTTA TGAGTACGGC CAGAGCCTTG CTGGTGGTAC CGTGCCCGTC 
AGCAATCCTC ACCTGGCAGC GTACAAGCTT CAGCACGCAT ACGCCCTGGC CGAGTATGGC 
TTTAGAGACA AGGCCCTTCA ATACTGCGAG GCTATCACGG CTGCGATCAC CGCGCAGACG 
AAGCGCTCTC CTTATTACCA TCCCATCCTT GAGGCCTATG TCGATGACTT GATGAAGCGA 
TTGAGGCAGG CACCCAAGGA GGAATCAAAC TCGTGGATCC CGAAGCCTAG CATGAATAAG 
GTATCTGATT CCATGTGGAA TCGGTTCAAC AAGTTTGTCG CTGGAGATGA CAACGAGGAT 
GGCAGTAAGG GCTCACCTGA CGCTGCGGGA GAATCTGGTC CCTTTGCACG AATCGCCGGA 
GGAACCCCGA CTATCAGTCG CTCGCCCTCT GTCAACAATC TTGAGACCTT TGGCGCGACC 
ATCCCCAGCT ATGGCATGCC TAGTGCTCCC GTGACGAACG GTCCTAATAT GTTCTCTCCT 
CCTCCTCCTA CACGGACGGC TTCACGCTAC GCACCAGGCG CTCCTCAACC ATCGACTCCG 
AACTATAACC CCTATGAGAC AAACTCTCCA TATGCGCCTC GCTCATCAAT GGAACGGGCC 
TCAGGCGAGT ACAGCAGAAG TTCAGTTGAG CTTCCGCGTC AGTCTTTGGA CTCTCAGCGG 
GGCTATTCGC ACAGCAGCTA TGCTCCCAAC CGTACATCTT CGCCTGCTCA GCCATATACA 
CCTTACGGCA CGACGCCACA GGAATCATCC TACTCTCTGC ATAACATGCA GCCTCAGCAA 
AGTTTGACAT CCCCAGCGGC TACTACTTCT GGTTATCAAC CCTTCACTCC ACAAAACAAC 
GTCTCGGCAA ATGATGAGCC CTCTAACGAA CCCCCTTCGG CCCCGTCAAC TGGCTACCAG 
CCGCCGTCGT ACGGATATGA GCCTCCGTCA TTCACCCCTT ACGAGGCGCC GGCGACCAAT 
GACGAGGAGG AGGGTGCCTC CAAGGAGAAC GGTGACTCTA ACCAGGGTGG CGAAGGTGTC 
AACACCTTTG AGCCCCCTTC ATTCCAGCCA TATAGCTATG AACCGCCTTC TTACGAACCC 
GACACCCCAC CTTCGAAAGA TGACGATCAA TCTGAGGAAG AAAAGCCCAA GCCGAAGAAG 
AAGGGCCCTA TGTACGATGA CGATGACGAT GATTTCCCCG CGGCGCCGAA GCCTGCCGGC 
AAGTCCAAGG CTGAAATTGA CCGGGAGAAC GAGGAAATGG TCCGTCGGAT CGCCGAAGAA 
GAAGCCAAAC GCGCCGCAGA AGCCAAAGCC GCCAAGAAGG GCTGGGGCTT CACCAGCTGG 
TTCGCAAAGA AGGAGGCTGC CGCCGCCGAT GCCAACGCCG CCGGAAGCAG CCCCGGCAAG 
CCCATCCGTG CCAAGCTCGG CGAGGCCAAC TCGTTCTACT ACGACCCCGA GCAAAAGCGC 
TGGATCAACA AGAACGCCAG TCCCGAAGAC CAAGCCGCCA AGAAATCCAC GCCACCACCA 
CCCAAGGGCG GCATCCCCCG CTCATCCGCC TCCAGCCCTG CTCCTCCCAT GGGTATGGGC 
GTGGGCGGCG GTAGCGCGCC CAACACCCCC GGCCGCGCCA GCGCCCCGCC TACTGGTCCG 
CCTAGACCGG CGGCGCTCAT GCCTTCGGCG TCGGAGAGCA ATGTTGGGTC TGGCCCACCT 
AGCGCTGTTG GTCCGCTGAG TCCCAGTGGA AGTAACGGGC CCCCAAGTGC GGGCTTGCTT 
TCCCCTGGCA TGGGTCCGGC GGCGATGCAG CGCCCGGCCT CGACGAGCAC GAGCGGGCCC 
CCTGCTGGTA CTAGTAAGCC GTTGAGCGCG ACGAGTAGTA TTGATGATTT GTTGGGAGCG 
GCGGTACCGA GGAAGAGGGG TGAGGCGAAG AAGCCGAGGA AGGCGGCTAG GTATGTGGAT 
GTTATGCAGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394600.1
CDS	369..6623
Translation	MASWNPAFMPESAADLPAPLPTDNTVADSTDHANGVAQDGSDFWGIDGGEEDVPAQNGASDSWFPDYGTGSHTTLPATDAQATSQITSETDTDTPDTATEQTETAHVTTEDAEAVEPAPTQTDEAPEPIVENAQATEAVPGDAATVDLVPEHVEPTDATTTEKADTDEPSDVASKHTSTMSFTRTIPHEPSWSDDGDPEWNLARADTDPFKFLPSSDRTNSFPPVPPLEQHEQQQEEQQENQQVQQVQQQQQQPQTDPHLEQSAIVQPQVANFLGDDDEVEVQGDDGFFSQLSEAQNDGFDQFGAQDANNSQQYMVGDLGAKATEALDARFEEGVPLIAHDGNSTAEREDTKDLFGGEDANDEDDFFSQVQQGSVNQADFEAQPLERKSTMQVLGAMDMGSVQPSFPPVEEQPEEEAVATVETQTHDHPEITSTDLNTGEGHEEQKPAGEDVEAKWKELFADDEEDTLSLLDDSTEASNEIKEKEIDPAAFFGSDDEGFLDDSEEFPTMEETTQPRAPSVAGSNTTTTPTTRYMPQTQTPSLTGAPNPYAPTAPPLTSAPSNPYFPAAPSAVAQTPSVTPFGAPVSAPPPSGRFGYGAVPPPQIERKAQSFVDKKGGYQSPYDLPMEVVKVKKRPSTATLNKPVTAPAAPPPSKSATHLPPPPPPTSVPHSSQPGVKPPPPRGEAKPKEKFFEELPVVTKARPASRNTLGTASPAQSSPYGPPPPAGPPSIASQPPVPGMPSAMGSVPNSSFTQHAEVPNLVAPPRVNPYASIQPSTTSLAPAVPAPASSRYSPAPSSGSQLSTPVPPAAASRYSPAPPASRPTSSGYTPTTSVPPVLPPVLPHQPRTSSPLAHFESRPLVSSHAESGLAEKRSSSSLYDLRLQRVPSLPPTQEVEEEGPMQGMVSPNQVPASHQTSPTASRHGAMPHRSRRTPPPQSLTVQPVTSPQRAAYSYQPAASAPSHEFAPPPRSQTQSPGALYGNRNVKPIEPIPRPSSVTDATSPRAALYSPITMQPSSTFPPISTITSRPRGNTQNFNLVPPTDGREHDPLQRWRGVPLISWGVGGALVTMFPKNVPRYGMGQTAPMVIRSPGEVKVKSMKDIEPMEERLAKFPGPLKGKSKKKETIAWLTNGIEMLERTVPHNLSHQLNPSHDDKRTTERLLLWKILRVFVEHDGVLEGNPTVNQAVREILYPEASTIGGQPEFANALNPSGMGNSATTSLQADSVDSSAVEQIRNHLISGDNEKAVWAAVDKRLWGHAFLLANALNPDLYKRVAQEFVKNEVNSTGHNNESLAALYDVLSGNHEESVDELVPAHARAGLQLVAKNSSSGPSKDAMGGLDKWRETLSLILSNRTADDARAINFLGNLLSGYGRAEAAHICFLFARSQTIFGGLDNPNSNFVLVGSDHRRQADHFAKEIEPLLLSEVYEYGQSLAGGTVPVSNPHLAAYKLQHAYALAEYGFRDKALQYCEAITAAITAQTKRSPYYHPILEAYVDDLMKRLRQAPKEESNSWIPKPSMNKVSDSMWNRFNKFVAGDDNEDGSKGSPDAAGESGPFARIAGGTPTISRSPSVNNLETFGATIPSYGMPSAPVTNGPNMFSPPPPTRTASRYAPGAPQPSTPNYNPYETNSPYAPRSSMERASGEYSRSSVELPRQSLDSQRGYSHSSYAPNRTSSPAQPYTPYGTTPQESSYSLHNMQPQQSLTSPAATTSGYQPFTPQNNVSANDEPSNEPPSAPSTGYQPPSYGYEPPSFTPYEAPATNDEEEGASKENGDSNQGGEGVNTFEPPSFQPYSYEPPSYEPDTPPSKDDDQSEEEKPKPKKKGPMYDDDDDDFPAAPKPAGKSKAEIDRENEEMVRRIAEEEAKRAAEAKAAKKGWGFTSWFAKKEAAAADANAAGSSPGKPIRAKLGEANSFYYDPEQKRWINKNASPEDQAAKKSTPPPPKGGIPRSSASSPAPPMGMGVGGGSAPNTPGRASAPPTGPPRPAALMPSASESNVGSGPPSAVGPLSPSGSNGPPSAGLLSPGMGPAAMQRPASTSTSGPPAGTSKPLSATSSIDDLLGAAVPRKRGEAKKPRKAARYVDVMQK

Target ORF information:

RefSeq Version	XM_011396298.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A COPII coat assembly protein sec-16 (NCU03819), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396298.1

ORF Insert Sequence:

ATGGCTTCAT GGAACCCGGC ATTCATGCCA GAATCGGCCG CCGACCTCCC GGCCCCCTTG 
CCGACTGATA ACACCGTCGC CGACTCAACA GATCATGCGA ACGGTGTAGC CCAAGATGGC 
TCCGACTTCT GGGGAATTGA TGGAGGAGAA GAGGACGTGC CCGCCCAGAA TGGCGCCTCT 
GACTCCTGGT TTCCCGACTA TGGAACAGGC AGTCATACGA CACTACCCGC GACTGATGCG 
CAAGCTACGA GCCAAATAAC CAGTGAAACT GATACCGACA CCCCCGATAC CGCTACCGAA 
CAGACCGAGA CCGCACATGT AACTACGGAA GATGCTGAAG CTGTCGAGCC AGCTCCCACT 
CAGACGGACG AAGCTCCCGA GCCCATCGTA GAGAACGCGC AAGCTACCGA GGCCGTCCCA 
GGAGACGCTG CCACCGTTGA CCTCGTCCCC GAACATGTGG AGCCTACCGA TGCTACTACT 
ACAGAAAAGG CCGATACAGA CGAGCCATCG GATGTCGCTT CCAAGCACAC GAGTACGATG 
TCCTTCACTC GAACCATTCC CCATGAGCCG AGCTGGAGCG ATGACGGCGA TCCTGAGTGG 
AACCTCGCGC GAGCGGATAC GGATCCCTTC AAGTTCTTGC CATCGAGCGA CCGAACCAAC 
TCTTTTCCTC CTGTTCCTCC CCTAGAACAG CACGAGCAAC AGCAGGAAGA GCAACAAGAA 
AATCAACAGG TACAGCAGGT ACAGCAGCAG CAGCAGCAAC CTCAAACCGA TCCTCATTTG 
GAGCAGTCAG CAATTGTCCA GCCCCAGGTC GCAAATTTCC TTGGTGATGA CGATGAGGTC 
GAGGTACAGG GCGATGATGG GTTCTTCAGC CAGTTGTCTG AGGCACAGAA CGATGGATTT 
GATCAGTTTG GCGCCCAAGA CGCGAACAAT TCTCAGCAGT ACATGGTTGG TGACCTAGGT 
GCTAAGGCCA CTGAGGCGCT TGATGCCCGT TTTGAAGAAG GTGTTCCCCT GATCGCACAT 
GATGGAAACT CGACAGCCGA GCGGGAAGAT ACAAAGGACC TATTCGGAGG AGAGGATGCC 
AATGATGAGG ACGACTTTTT CAGCCAGGTT CAGCAAGGGA GCGTCAACCA AGCTGACTTT 
GAGGCGCAGC CGCTCGAACG CAAGTCGACG ATGCAAGTAT TGGGCGCTAT GGATATGGGC 
TCCGTCCAGC CAAGCTTCCC ACCAGTGGAA GAGCAGCCCG AGGAAGAGGC CGTCGCCACG 
GTAGAGACGC AAACCCACGA CCATCCCGAG ATAACGAGTA CAGATCTGAA CACAGGTGAA 
GGTCACGAGG AGCAGAAGCC AGCAGGCGAA GACGTTGAGG CCAAGTGGAA GGAACTGTTT 
GCTGACGATG AAGAGGACAC CCTATCGCTT CTTGATGATT CCACAGAGGC GTCGAATGAG 
ATCAAGGAGA AGGAGATTGA TCCGGCTGCG TTCTTTGGGA GTGATGATGA GGGCTTTCTC 
GACGATTCGG AAGAGTTCCC AACAATGGAG GAAACAACCC AGCCCAGAGC GCCTAGCGTA 
GCTGGCTCCA ACACCACGAC AACCCCTACC ACCCGGTACA TGCCTCAGAC CCAAACTCCA 
AGCCTGACCG GAGCCCCGAA TCCCTATGCC CCGACTGCGC CACCCCTAAC TTCGGCGCCG 
TCAAATCCTT ACTTTCCTGC CGCACCCAGC GCGGTTGCGC AGACGCCGTC TGTTACGCCC 
TTTGGCGCCC CTGTCTCGGC TCCACCTCCA AGCGGGAGAT TTGGATACGG CGCGGTCCCT 
CCGCCACAGA TTGAGAGGAA AGCCCAAAGC TTCGTCGACA AGAAGGGCGG CTACCAGTCA 
CCGTACGACC TTCCTATGGA GGTAGTCAAG GTTAAGAAGC GCCCAAGTAC GGCAACTCTT 
AACAAGCCTG TGACAGCACC GGCCGCTCCA CCGCCTTCCA AAAGCGCTAC TCACCTGCCT 
CCTCCACCAC CTCCTACCTC TGTTCCACAC TCATCTCAGC CTGGTGTCAA GCCACCACCG 
CCTCGTGGTG AAGCCAAGCC GAAGGAGAAG TTCTTTGAAG AGCTTCCTGT GGTCACAAAG 
GCGCGCCCAG CTTCTCGAAA TACATTGGGC ACCGCATCGC CGGCACAGTC TAGCCCATAC 
GGCCCACCGC CCCCGGCTGG CCCACCTTCT ATCGCCTCGC AGCCACCAGT ACCTGGAATG 
CCGTCTGCCA TGGGATCTGT TCCCAACTCT TCCTTTACCC AGCATGCGGA GGTTCCCAAC 
CTGGTTGCGC CGCCACGTGT CAACCCTTAT GCTTCGATAC AGCCCAGCAC TACGAGCCTT 
GCTCCCGCTG TTCCTGCCCC GGCTTCAAGT CGTTATTCTC CAGCGCCTTC CAGTGGGTCA 
CAGCTCAGCA CTCCCGTCCC ACCTGCTGCG GCAAGCCGTT ACTCTCCCGC CCCGCCAGCT 
TCTCGGCCAA CGAGTTCGGG TTATACTCCG ACTACTTCGG TGCCACCGGT TCTACCACCG 
GTTTTGCCGC ATCAACCCCG TACCTCAAGT CCGCTAGCAC ATTTCGAGTC CAGGCCTCTT 
GTTTCTAGCC ATGCAGAAAG TGGCCTGGCG GAAAAGCGGA GCAGCAGCTC GTTGTACGAC 
TTGCGACTGC AGAGAGTGCC TTCTCTACCT CCGACTCAGG AGGTGGAAGA AGAAGGCCCC 
ATGCAGGGTA TGGTGTCCCC CAACCAAGTC CCGGCATCCC ACCAGACCTC TCCTACGGCG 
TCAAGACATG GCGCTATGCC CCACCGTTCT AGAAGGACAC CGCCTCCGCA GTCTCTAACT 
GTGCAGCCGG TGACGTCCCC TCAGAGGGCG GCATACAGCT ATCAGCCTGC GGCTAGTGCT 
CCATCTCACG AATTCGCGCC ACCACCTCGG TCGCAGACTC AATCCCCTGG TGCCCTCTAC 
GGGAACAGGA ATGTAAAGCC TATTGAACCT ATCCCTCGTC CTTCGTCGGT CACTGACGCG 
ACTTCGCCGC GGGCTGCGTT GTATTCTCCT ATCACCATGC AACCCAGTTC TACTTTCCCC 
CCCATTTCTA CGATTACCTC GCGGCCGCGT GGTAACACTC AGAATTTTAA CCTAGTTCCC 
CCGACAGATG GTAGAGAACA TGATCCCCTA CAGAGATGGC GTGGTGTTCC TCTCATTTCC 
TGGGGCGTAG GTGGAGCTCT GGTAACAATG TTCCCCAAGA ACGTGCCACG ATATGGCATG 
GGCCAAACAG CCCCCATGGT TATTCGTAGC CCAGGAGAAG TCAAGGTCAA GAGCATGAAG 
GACATAGAGC CAATGGAGGA GCGCTTGGCC AAATTCCCTG GGCCTCTAAA GGGGAAGTCG 
AAGAAGAAGG AGACCATTGC CTGGTTAACA AACGGAATTG AGATGCTGGA GAGGACTGTG 
CCACATAACC TGTCTCATCA GCTCAACCCG TCTCACGATG ACAAGCGGAC AACAGAGAGA 
CTCTTGCTTT GGAAGATTCT GAGGGTCTTT GTCGAGCATG ATGGCGTGTT GGAAGGAAAC 
CCGACCGTTA ACCAAGCTGT GCGCGAAATC CTGTACCCGG AGGCTTCAAC CATTGGTGGT 
CAGCCTGAGT TCGCAAATGC TCTGAACCCA TCAGGAATGG GCAATTCTGC CACGACTTCA 
CTACAAGCCG ACTCGGTTGA TTCTTCTGCT GTTGAGCAGA TCCGCAACCA CCTCATCAGT 
GGCGACAATG AGAAGGCAGT GTGGGCTGCG GTCGATAAGC GGCTTTGGGG CCATGCCTTC 
CTGCTGGCCA ACGCCCTTAA CCCTGACCTC TATAAGCGGG TCGCTCAAGA ATTTGTGAAG 
AACGAGGTGA ATTCCACCGG TCATAACAAT GAGTCGCTTG CTGCTCTCTA TGATGTCCTC 
TCTGGCAACC ACGAAGAAAG CGTGGACGAG CTGGTGCCCG CCCATGCTCG AGCTGGTCTT 
CAGCTAGTGG CCAAAAATTC ATCTTCTGGT CCCTCCAAGG ACGCCATGGG TGGTCTTGAT 
AAGTGGCGCG AAACCCTCTC CCTGATCCTC AGCAACAGGA CTGCTGATGA TGCCCGAGCC 
ATCAACTTTT TGGGCAATTT GCTATCAGGC TATGGAAGAG CCGAGGCGGC TCATATTTGC 
TTCTTGTTTG CCAGAAGTCA AACCATCTTC GGCGGCTTGG ACAACCCGAA CTCTAACTTC 
GTTCTTGTTG GGTCTGACCA TAGGCGGCAG GCGGACCATT TCGCAAAGGA GATTGAGCCT 
CTTTTGTTGA GCGAGGTTTA TGAGTACGGC CAGAGCCTTG CTGGTGGTAC CGTGCCCGTC 
AGCAATCCTC ACCTGGCAGC GTACAAGCTT CAGCACGCAT ACGCCCTGGC CGAGTATGGC 
TTTAGAGACA AGGCCCTTCA ATACTGCGAG GCTATCACGG CTGCGATCAC CGCGCAGACG 
AAGCGCTCTC CTTATTACCA TCCCATCCTT GAGGCCTATG TCGATGACTT GATGAAGCGA 
TTGAGGCAGG CACCCAAGGA GGAATCAAAC TCGTGGATCC CGAAGCCTAG CATGAATAAG 
GTATCTGATT CCATGTGGAA TCGGTTCAAC AAGTTTGTCG CTGGAGATGA CAACGAGGAT 
GGCAGTAAGG GCTCACCTGA CGCTGCGGGA GAATCTGGTC CCTTTGCACG AATCGCCGGA 
GGAACCCCGA CTATCAGTCG CTCGCCCTCT GTCAACAATC TTGAGACCTT TGGCGCGACC 
ATCCCCAGCT ATGGCATGCC TAGTGCTCCC GTGACGAACG GTCCTAATAT GTTCTCTCCT 
CCTCCTCCTA CACGGACGGC TTCACGCTAC GCACCAGGCG CTCCTCAACC ATCGACTCCG 
AACTATAACC CCTATGAGAC AAACTCTCCA TATGCGCCTC GCTCATCAAT GGAACGGGCC 
TCAGGCGAGT ACAGCAGAAG TTCAGTTGAG CTTCCGCGTC AGTCTTTGGA CTCTCAGCGG 
GGCTATTCGC ACAGCAGCTA TGCTCCCAAC CGTACATCTT CGCCTGCTCA GCCATATACA 
CCTTACGGCA CGACGCCACA GGAATCATCC TACTCTCTGC ATAACATGCA GCCTCAGCAA 
AGTTTGACAT CCCCAGCGGC TACTACTTCT GGTTATCAAC CCTTCACTCC ACAAAACAAC 
GTCTCGGCAA ATGATGAGCC CTCTAACGAA CCCCCTTCGG CCCCGTCAAC TGGCTACCAG 
CCGCCGTCGT ACGGATATGA GCCTCCGTCA TTCACCCCTT ACGAGGCGCC GGCGACCAAT 
GACGAGGAGG AGGGTGCCTC CAAGGAGAAC GGTGACTCTA ACCAGGGTGG CGAAGGTGTC 
AACACCTTTG AGCCCCCTTC ATTCCAGCCA TATAGCTATG AACCGCCTTC TTACGAACCC 
GACACCCCAC CTTCGAAAGA TGACGATCAA TCTGAGGAAG AAAAGCCCAA GCCGAAGAAG 
AAGGGCCCTA TGTACGATGA CGATGACGAT GATTTCCCCG CGGCGCCGAA GCCTGCCGGC 
AAGTCCAAGG CTGAAATTGA CCGGGAGAAC GAGGAAATGG TCCGTCGGAT CGCCGAAGAA 
GAAGCCAAAC GCGCCGCAGA AGCCAAAGCC GCCAAGAAGG GCTGGGGCTT CACCAGCTGG 
TTCGCAAAGA AGGAGGCTGC CGCCGCCGAT GCCAACGCCG CCGGAAGCAG CCCCGGCAAG 
CCCATCCGTG CCAAGCTCGG CGAGGCCAAC TCGTTCTACT ACGACCCCGA GCAAAAGCGC 
TGGATCAACA AGAACGCCAG TCCCGAAGAC CAAGCCGCCA AGAAATCCAC GCCACCACCA 
CCCAAGGGCG GCATCCCCCG CTCATCCGCC TCCAGCCCTG CTCCTCCCAT GGGTATGGGC 
GTGGGCGGCG GTAGCGCGCC CAACACCCCC GGCCGCGCCA GCGCCCCGCC TACTGGTCCG 
CCTAGACCGG CGGCGCTCAT GCCTTCGGCG TCGGAGAGCA ATGTTGGGTC TGGCCCACCT 
AGCGCTGTTG GTCCGCTGAG TCCCAGTGGA AGTAACGGGC CCCCAAGTGC GGGCTTGCTT 
TCCCCTGGCA TGGGTCCGGC GGCGATGCAG CGCCCGGCCT CGACGAGCAC GAGCGGGCCC 
CCTGCTGGTA CTAGTAAGCC GTTGAGCGCG ACGAGTAGTA TTGATGATTT GTTGGGAGCG 
GCGGTACCGA GGAAGAGGGG TGAGGCGAAG AAGCCGAGGA AGGCGGCTAG GTATGTGGAT 
GTTATGCAGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU03819 ( XM_011396297.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394599.1 Neurospora crassa OR74A COPII coat assembly protein sec-16, variant 1 (NCU03819), mRNA.

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CloneID	ONf11694
Clone ID Related Accession (Same CDS sequence)	XM_011396299.1 , XM_011396298.1 , XM_011396297.1
Accession Version	XM_011396297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	COPII coat assembly protein sec-16, variant 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026505).

ATGGCTTCAT GGAACCCGGC ATTCATGCCA GAATCGGCCG CCGACCTCCC GGCCCCCTTG 
CCGACTGATA ACACCGTCGC CGACTCAACA GATCATGCGA ACGGTGTAGC CCAAGATGGC 
TCCGACTTCT GGGGAATTGA TGGAGGAGAA GAGGACGTGC CCGCCCAGAA TGGCGCCTCT 
GACTCCTGGT TTCCCGACTA TGGAACAGGC AGTCATACGA CACTACCCGC GACTGATGCG 
CAAGCTACGA GCCAAATAAC CAGTGAAACT GATACCGACA CCCCCGATAC CGCTACCGAA 
CAGACCGAGA CCGCACATGT AACTACGGAA GATGCTGAAG CTGTCGAGCC AGCTCCCACT 
CAGACGGACG AAGCTCCCGA GCCCATCGTA GAGAACGCGC AAGCTACCGA GGCCGTCCCA 
GGAGACGCTG CCACCGTTGA CCTCGTCCCC GAACATGTGG AGCCTACCGA TGCTACTACT 
ACAGAAAAGG CCGATACAGA CGAGCCATCG GATGTCGCTT CCAAGCACAC GAGTACGATG 
TCCTTCACTC GAACCATTCC CCATGAGCCG AGCTGGAGCG ATGACGGCGA TCCTGAGTGG 
AACCTCGCGC GAGCGGATAC GGATCCCTTC AAGTTCTTGC CATCGAGCGA CCGAACCAAC 
TCTTTTCCTC CTGTTCCTCC CCTAGAACAG CACGAGCAAC AGCAGGAAGA GCAACAAGAA 
AATCAACAGG TACAGCAGGT ACAGCAGCAG CAGCAGCAAC CTCAAACCGA TCCTCATTTG 
GAGCAGTCAG CAATTGTCCA GCCCCAGGTC GCAAATTTCC TTGGTGATGA CGATGAGGTC 
GAGGTACAGG GCGATGATGG GTTCTTCAGC CAGTTGTCTG AGGCACAGAA CGATGGATTT 
GATCAGTTTG GCGCCCAAGA CGCGAACAAT TCTCAGCAGT ACATGGTTGG TGACCTAGGT 
GCTAAGGCCA CTGAGGCGCT TGATGCCCGT TTTGAAGAAG GTGTTCCCCT GATCGCACAT 
GATGGAAACT CGACAGCCGA GCGGGAAGAT ACAAAGGACC TATTCGGAGG AGAGGATGCC 
AATGATGAGG ACGACTTTTT CAGCCAGGTT CAGCAAGGGA GCGTCAACCA AGCTGACTTT 
GAGGCGCAGC CGCTCGAACG CAAGTCGACG ATGCAAGTAT TGGGCGCTAT GGATATGGGC 
TCCGTCCAGC CAAGCTTCCC ACCAGTGGAA GAGCAGCCCG AGGAAGAGGC CGTCGCCACG 
GTAGAGACGC AAACCCACGA CCATCCCGAG ATAACGAGTA CAGATCTGAA CACAGGTGAA 
GGTCACGAGG AGCAGAAGCC AGCAGGCGAA GACGTTGAGG CCAAGTGGAA GGAACTGTTT 
GCTGACGATG AAGAGGACAC CCTATCGCTT CTTGATGATT CCACAGAGGC GTCGAATGAG 
ATCAAGGAGA AGGAGATTGA TCCGGCTGCG TTCTTTGGGA GTGATGATGA GGGCTTTCTC 
GACGATTCGG AAGAGTTCCC AACAATGGAG GAAACAACCC AGCCCAGAGC GCCTAGCGTA 
GCTGGCTCCA ACACCACGAC AACCCCTACC ACCCGGTACA TGCCTCAGAC CCAAACTCCA 
AGCCTGACCG GAGCCCCGAA TCCCTATGCC CCGACTGCGC CACCCCTAAC TTCGGCGCCG 
TCAAATCCTT ACTTTCCTGC CGCACCCAGC GCGGTTGCGC AGACGCCGTC TGTTACGCCC 
TTTGGCGCCC CTGTCTCGGC TCCACCTCCA AGCGGGAGAT TTGGATACGG CGCGGTCCCT 
CCGCCACAGA TTGAGAGGAA AGCCCAAAGC TTCGTCGACA AGAAGGGCGG CTACCAGTCA 
CCGTACGACC TTCCTATGGA GGTAGTCAAG GTTAAGAAGC GCCCAAGTAC GGCAACTCTT 
AACAAGCCTG TGACAGCACC GGCCGCTCCA CCGCCTTCCA AAAGCGCTAC TCACCTGCCT 
CCTCCACCAC CTCCTACCTC TGTTCCACAC TCATCTCAGC CTGGTGTCAA GCCACCACCG 
CCTCGTGGTG AAGCCAAGCC GAAGGAGAAG TTCTTTGAAG AGCTTCCTGT GGTCACAAAG 
GCGCGCCCAG CTTCTCGAAA TACATTGGGC ACCGCATCGC CGGCACAGTC TAGCCCATAC 
GGCCCACCGC CCCCGGCTGG CCCACCTTCT ATCGCCTCGC AGCCACCAGT ACCTGGAATG 
CCGTCTGCCA TGGGATCTGT TCCCAACTCT TCCTTTACCC AGCATGCGGA GGTTCCCAAC 
CTGGTTGCGC CGCCACGTGT CAACCCTTAT GCTTCGATAC AGCCCAGCAC TACGAGCCTT 
GCTCCCGCTG TTCCTGCCCC GGCTTCAAGT CGTTATTCTC CAGCGCCTTC CAGTGGGTCA 
CAGCTCAGCA CTCCCGTCCC ACCTGCTGCG GCAAGCCGTT ACTCTCCCGC CCCGCCAGCT 
TCTCGGCCAA CGAGTTCGGG TTATACTCCG ACTACTTCGG TGCCACCGGT TCTACCACCG 
GTTTTGCCGC ATCAACCCCG TACCTCAAGT CCGCTAGCAC ATTTCGAGTC CAGGCCTCTT 
GTTTCTAGCC ATGCAGAAAG TGGCCTGGCG GAAAAGCGGA GCAGCAGCTC GTTGTACGAC 
TTGCGACTGC AGAGAGTGCC TTCTCTACCT CCGACTCAGG AGGTGGAAGA AGAAGGCCCC 
ATGCAGGGTA TGGTGTCCCC CAACCAAGTC CCGGCATCCC ACCAGACCTC TCCTACGGCG 
TCAAGACATG GCGCTATGCC CCACCGTTCT AGAAGGACAC CGCCTCCGCA GTCTCTAACT 
GTGCAGCCGG TGACGTCCCC TCAGAGGGCG GCATACAGCT ATCAGCCTGC GGCTAGTGCT 
CCATCTCACG AATTCGCGCC ACCACCTCGG TCGCAGACTC AATCCCCTGG TGCCCTCTAC 
GGGAACAGGA ATGTAAAGCC TATTGAACCT ATCCCTCGTC CTTCGTCGGT CACTGACGCG 
ACTTCGCCGC GGGCTGCGTT GTATTCTCCT ATCACCATGC AACCCAGTTC TACTTTCCCC 
CCCATTTCTA CGATTACCTC GCGGCCGCGT GGTAACACTC AGAATTTTAA CCTAGTTCCC 
CCGACAGATG GTAGAGAACA TGATCCCCTA CAGAGATGGC GTGGTGTTCC TCTCATTTCC 
TGGGGCGTAG GTGGAGCTCT GGTAACAATG TTCCCCAAGA ACGTGCCACG ATATGGCATG 
GGCCAAACAG CCCCCATGGT TATTCGTAGC CCAGGAGAAG TCAAGGTCAA GAGCATGAAG 
GACATAGAGC CAATGGAGGA GCGCTTGGCC AAATTCCCTG GGCCTCTAAA GGGGAAGTCG 
AAGAAGAAGG AGACCATTGC CTGGTTAACA AACGGAATTG AGATGCTGGA GAGGACTGTG 
CCACATAACC TGTCTCATCA GCTCAACCCG TCTCACGATG ACAAGCGGAC AACAGAGAGA 
CTCTTGCTTT GGAAGATTCT GAGGGTCTTT GTCGAGCATG ATGGCGTGTT GGAAGGAAAC 
CCGACCGTTA ACCAAGCTGT GCGCGAAATC CTGTACCCGG AGGCTTCAAC CATTGGTGGT 
CAGCCTGAGT TCGCAAATGC TCTGAACCCA TCAGGAATGG GCAATTCTGC CACGACTTCA 
CTACAAGCCG ACTCGGTTGA TTCTTCTGCT GTTGAGCAGA TCCGCAACCA CCTCATCAGT 
GGCGACAATG AGAAGGCAGT GTGGGCTGCG GTCGATAAGC GGCTTTGGGG CCATGCCTTC 
CTGCTGGCCA ACGCCCTTAA CCCTGACCTC TATAAGCGGG TCGCTCAAGA ATTTGTGAAG 
AACGAGGTGA ATTCCACCGG TCATAACAAT GAGTCGCTTG CTGCTCTCTA TGATGTCCTC 
TCTGGCAACC ACGAAGAAAG CGTGGACGAG CTGGTGCCCG CCCATGCTCG AGCTGGTCTT 
CAGCTAGTGG CCAAAAATTC ATCTTCTGGT CCCTCCAAGG ACGCCATGGG TGGTCTTGAT 
AAGTGGCGCG AAACCCTCTC CCTGATCCTC AGCAACAGGA CTGCTGATGA TGCCCGAGCC 
ATCAACTTTT TGGGCAATTT GCTATCAGGC TATGGAAGAG CCGAGGCGGC TCATATTTGC 
TTCTTGTTTG CCAGAAGTCA AACCATCTTC GGCGGCTTGG ACAACCCGAA CTCTAACTTC 
GTTCTTGTTG GGTCTGACCA TAGGCGGCAG GCGGACCATT TCGCAAAGGA GATTGAGCCT 
CTTTTGTTGA GCGAGGTTTA TGAGTACGGC CAGAGCCTTG CTGGTGGTAC CGTGCCCGTC 
AGCAATCCTC ACCTGGCAGC GTACAAGCTT CAGCACGCAT ACGCCCTGGC CGAGTATGGC 
TTTAGAGACA AGGCCCTTCA ATACTGCGAG GCTATCACGG CTGCGATCAC CGCGCAGACG 
AAGCGCTCTC CTTATTACCA TCCCATCCTT GAGGCCTATG TCGATGACTT GATGAAGCGA 
TTGAGGCAGG CACCCAAGGA GGAATCAAAC TCGTGGATCC CGAAGCCTAG CATGAATAAG 
GTATCTGATT CCATGTGGAA TCGGTTCAAC AAGTTTGTCG CTGGAGATGA CAACGAGGAT 
GGCAGTAAGG GCTCACCTGA CGCTGCGGGA GAATCTGGTC CCTTTGCACG AATCGCCGGA 
GGAACCCCGA CTATCAGTCG CTCGCCCTCT GTCAACAATC TTGAGACCTT TGGCGCGACC 
ATCCCCAGCT ATGGCATGCC TAGTGCTCCC GTGACGAACG GTCCTAATAT GTTCTCTCCT 
CCTCCTCCTA CACGGACGGC TTCACGCTAC GCACCAGGCG CTCCTCAACC ATCGACTCCG 
AACTATAACC CCTATGAGAC AAACTCTCCA TATGCGCCTC GCTCATCAAT GGAACGGGCC 
TCAGGCGAGT ACAGCAGAAG TTCAGTTGAG CTTCCGCGTC AGTCTTTGGA CTCTCAGCGG 
GGCTATTCGC ACAGCAGCTA TGCTCCCAAC CGTACATCTT CGCCTGCTCA GCCATATACA 
CCTTACGGCA CGACGCCACA GGAATCATCC TACTCTCTGC ATAACATGCA GCCTCAGCAA 
AGTTTGACAT CCCCAGCGGC TACTACTTCT GGTTATCAAC CCTTCACTCC ACAAAACAAC 
GTCTCGGCAA ATGATGAGCC CTCTAACGAA CCCCCTTCGG CCCCGTCAAC TGGCTACCAG 
CCGCCGTCGT ACGGATATGA GCCTCCGTCA TTCACCCCTT ACGAGGCGCC GGCGACCAAT 
GACGAGGAGG AGGGTGCCTC CAAGGAGAAC GGTGACTCTA ACCAGGGTGG CGAAGGTGTC 
AACACCTTTG AGCCCCCTTC ATTCCAGCCA TATAGCTATG AACCGCCTTC TTACGAACCC 
GACACCCCAC CTTCGAAAGA TGACGATCAA TCTGAGGAAG AAAAGCCCAA GCCGAAGAAG 
AAGGGCCCTA TGTACGATGA CGATGACGAT GATTTCCCCG CGGCGCCGAA GCCTGCCGGC 
AAGTCCAAGG CTGAAATTGA CCGGGAGAAC GAGGAAATGG TCCGTCGGAT CGCCGAAGAA 
GAAGCCAAAC GCGCCGCAGA AGCCAAAGCC GCCAAGAAGG GCTGGGGCTT CACCAGCTGG 
TTCGCAAAGA AGGAGGCTGC CGCCGCCGAT GCCAACGCCG CCGGAAGCAG CCCCGGCAAG 
CCCATCCGTG CCAAGCTCGG CGAGGCCAAC TCGTTCTACT ACGACCCCGA GCAAAAGCGC 
TGGATCAACA AGAACGCCAG TCCCGAAGAC CAAGCCGCCA AGAAATCCAC GCCACCACCA 
CCCAAGGGCG GCATCCCCCG CTCATCCGCC TCCAGCCCTG CTCCTCCCAT GGGTATGGGC 
GTGGGCGGCG GTAGCGCGCC CAACACCCCC GGCCGCGCCA GCGCCCCGCC TACTGGTCCG 
CCTAGACCGG CGGCGCTCAT GCCTTCGGCG TCGGAGAGCA ATGTTGGGTC TGGCCCACCT 
AGCGCTGTTG GTCCGCTGAG TCCCAGTGGA AGTAACGGGC CCCCAAGTGC GGGCTTGCTT 
TCCCCTGGCA TGGGTCCGGC GGCGATGCAG CGCCCGGCCT CGACGAGCAC GAGCGGGCCC 
CCTGCTGGTA CTAGTAAGCC GTTGAGCGCG ACGAGTAGTA TTGATGATTT GTTGGGAGCG 
GCGGTACCGA GGAAGAGGGG TGAGGCGAAG AAGCCGAGGA AGGCGGCTAG GTATGTGGAT 
GTTATGCAGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394599.1
CDS	309..6563
Translation	MASWNPAFMPESAADLPAPLPTDNTVADSTDHANGVAQDGSDFWGIDGGEEDVPAQNGASDSWFPDYGTGSHTTLPATDAQATSQITSETDTDTPDTATEQTETAHVTTEDAEAVEPAPTQTDEAPEPIVENAQATEAVPGDAATVDLVPEHVEPTDATTTEKADTDEPSDVASKHTSTMSFTRTIPHEPSWSDDGDPEWNLARADTDPFKFLPSSDRTNSFPPVPPLEQHEQQQEEQQENQQVQQVQQQQQQPQTDPHLEQSAIVQPQVANFLGDDDEVEVQGDDGFFSQLSEAQNDGFDQFGAQDANNSQQYMVGDLGAKATEALDARFEEGVPLIAHDGNSTAEREDTKDLFGGEDANDEDDFFSQVQQGSVNQADFEAQPLERKSTMQVLGAMDMGSVQPSFPPVEEQPEEEAVATVETQTHDHPEITSTDLNTGEGHEEQKPAGEDVEAKWKELFADDEEDTLSLLDDSTEASNEIKEKEIDPAAFFGSDDEGFLDDSEEFPTMEETTQPRAPSVAGSNTTTTPTTRYMPQTQTPSLTGAPNPYAPTAPPLTSAPSNPYFPAAPSAVAQTPSVTPFGAPVSAPPPSGRFGYGAVPPPQIERKAQSFVDKKGGYQSPYDLPMEVVKVKKRPSTATLNKPVTAPAAPPPSKSATHLPPPPPPTSVPHSSQPGVKPPPPRGEAKPKEKFFEELPVVTKARPASRNTLGTASPAQSSPYGPPPPAGPPSIASQPPVPGMPSAMGSVPNSSFTQHAEVPNLVAPPRVNPYASIQPSTTSLAPAVPAPASSRYSPAPSSGSQLSTPVPPAAASRYSPAPPASRPTSSGYTPTTSVPPVLPPVLPHQPRTSSPLAHFESRPLVSSHAESGLAEKRSSSSLYDLRLQRVPSLPPTQEVEEEGPMQGMVSPNQVPASHQTSPTASRHGAMPHRSRRTPPPQSLTVQPVTSPQRAAYSYQPAASAPSHEFAPPPRSQTQSPGALYGNRNVKPIEPIPRPSSVTDATSPRAALYSPITMQPSSTFPPISTITSRPRGNTQNFNLVPPTDGREHDPLQRWRGVPLISWGVGGALVTMFPKNVPRYGMGQTAPMVIRSPGEVKVKSMKDIEPMEERLAKFPGPLKGKSKKKETIAWLTNGIEMLERTVPHNLSHQLNPSHDDKRTTERLLLWKILRVFVEHDGVLEGNPTVNQAVREILYPEASTIGGQPEFANALNPSGMGNSATTSLQADSVDSSAVEQIRNHLISGDNEKAVWAAVDKRLWGHAFLLANALNPDLYKRVAQEFVKNEVNSTGHNNESLAALYDVLSGNHEESVDELVPAHARAGLQLVAKNSSSGPSKDAMGGLDKWRETLSLILSNRTADDARAINFLGNLLSGYGRAEAAHICFLFARSQTIFGGLDNPNSNFVLVGSDHRRQADHFAKEIEPLLLSEVYEYGQSLAGGTVPVSNPHLAAYKLQHAYALAEYGFRDKALQYCEAITAAITAQTKRSPYYHPILEAYVDDLMKRLRQAPKEESNSWIPKPSMNKVSDSMWNRFNKFVAGDDNEDGSKGSPDAAGESGPFARIAGGTPTISRSPSVNNLETFGATIPSYGMPSAPVTNGPNMFSPPPPTRTASRYAPGAPQPSTPNYNPYETNSPYAPRSSMERASGEYSRSSVELPRQSLDSQRGYSHSSYAPNRTSSPAQPYTPYGTTPQESSYSLHNMQPQQSLTSPAATTSGYQPFTPQNNVSANDEPSNEPPSAPSTGYQPPSYGYEPPSFTPYEAPATNDEEEGASKENGDSNQGGEGVNTFEPPSFQPYSYEPPSYEPDTPPSKDDDQSEEEKPKPKKKGPMYDDDDDDFPAAPKPAGKSKAEIDRENEEMVRRIAEEEAKRAAEAKAAKKGWGFTSWFAKKEAAAADANAAGSSPGKPIRAKLGEANSFYYDPEQKRWINKNASPEDQAAKKSTPPPPKGGIPRSSASSPAPPMGMGVGGGSAPNTPGRASAPPTGPPRPAALMPSASESNVGSGPPSAVGPLSPSGSNGPPSAGLLSPGMGPAAMQRPASTSTSGPPAGTSKPLSATSSIDDLLGAAVPRKRGEAKKPRKAARYVDVMQK

Target ORF information:

RefSeq Version	XM_011396297.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A COPII coat assembly protein sec-16, variant 1 (NCU03819), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396297.1

ORF Insert Sequence:

ATGGCTTCAT GGAACCCGGC ATTCATGCCA GAATCGGCCG CCGACCTCCC GGCCCCCTTG 
CCGACTGATA ACACCGTCGC CGACTCAACA GATCATGCGA ACGGTGTAGC CCAAGATGGC 
TCCGACTTCT GGGGAATTGA TGGAGGAGAA GAGGACGTGC CCGCCCAGAA TGGCGCCTCT 
GACTCCTGGT TTCCCGACTA TGGAACAGGC AGTCATACGA CACTACCCGC GACTGATGCG 
CAAGCTACGA GCCAAATAAC CAGTGAAACT GATACCGACA CCCCCGATAC CGCTACCGAA 
CAGACCGAGA CCGCACATGT AACTACGGAA GATGCTGAAG CTGTCGAGCC AGCTCCCACT 
CAGACGGACG AAGCTCCCGA GCCCATCGTA GAGAACGCGC AAGCTACCGA GGCCGTCCCA 
GGAGACGCTG CCACCGTTGA CCTCGTCCCC GAACATGTGG AGCCTACCGA TGCTACTACT 
ACAGAAAAGG CCGATACAGA CGAGCCATCG GATGTCGCTT CCAAGCACAC GAGTACGATG 
TCCTTCACTC GAACCATTCC CCATGAGCCG AGCTGGAGCG ATGACGGCGA TCCTGAGTGG 
AACCTCGCGC GAGCGGATAC GGATCCCTTC AAGTTCTTGC CATCGAGCGA CCGAACCAAC 
TCTTTTCCTC CTGTTCCTCC CCTAGAACAG CACGAGCAAC AGCAGGAAGA GCAACAAGAA 
AATCAACAGG TACAGCAGGT ACAGCAGCAG CAGCAGCAAC CTCAAACCGA TCCTCATTTG 
GAGCAGTCAG CAATTGTCCA GCCCCAGGTC GCAAATTTCC TTGGTGATGA CGATGAGGTC 
GAGGTACAGG GCGATGATGG GTTCTTCAGC CAGTTGTCTG AGGCACAGAA CGATGGATTT 
GATCAGTTTG GCGCCCAAGA CGCGAACAAT TCTCAGCAGT ACATGGTTGG TGACCTAGGT 
GCTAAGGCCA CTGAGGCGCT TGATGCCCGT TTTGAAGAAG GTGTTCCCCT GATCGCACAT 
GATGGAAACT CGACAGCCGA GCGGGAAGAT ACAAAGGACC TATTCGGAGG AGAGGATGCC 
AATGATGAGG ACGACTTTTT CAGCCAGGTT CAGCAAGGGA GCGTCAACCA AGCTGACTTT 
GAGGCGCAGC CGCTCGAACG CAAGTCGACG ATGCAAGTAT TGGGCGCTAT GGATATGGGC 
TCCGTCCAGC CAAGCTTCCC ACCAGTGGAA GAGCAGCCCG AGGAAGAGGC CGTCGCCACG 
GTAGAGACGC AAACCCACGA CCATCCCGAG ATAACGAGTA CAGATCTGAA CACAGGTGAA 
GGTCACGAGG AGCAGAAGCC AGCAGGCGAA GACGTTGAGG CCAAGTGGAA GGAACTGTTT 
GCTGACGATG AAGAGGACAC CCTATCGCTT CTTGATGATT CCACAGAGGC GTCGAATGAG 
ATCAAGGAGA AGGAGATTGA TCCGGCTGCG TTCTTTGGGA GTGATGATGA GGGCTTTCTC 
GACGATTCGG AAGAGTTCCC AACAATGGAG GAAACAACCC AGCCCAGAGC GCCTAGCGTA 
GCTGGCTCCA ACACCACGAC AACCCCTACC ACCCGGTACA TGCCTCAGAC CCAAACTCCA 
AGCCTGACCG GAGCCCCGAA TCCCTATGCC CCGACTGCGC CACCCCTAAC TTCGGCGCCG 
TCAAATCCTT ACTTTCCTGC CGCACCCAGC GCGGTTGCGC AGACGCCGTC TGTTACGCCC 
TTTGGCGCCC CTGTCTCGGC TCCACCTCCA AGCGGGAGAT TTGGATACGG CGCGGTCCCT 
CCGCCACAGA TTGAGAGGAA AGCCCAAAGC TTCGTCGACA AGAAGGGCGG CTACCAGTCA 
CCGTACGACC TTCCTATGGA GGTAGTCAAG GTTAAGAAGC GCCCAAGTAC GGCAACTCTT 
AACAAGCCTG TGACAGCACC GGCCGCTCCA CCGCCTTCCA AAAGCGCTAC TCACCTGCCT 
CCTCCACCAC CTCCTACCTC TGTTCCACAC TCATCTCAGC CTGGTGTCAA GCCACCACCG 
CCTCGTGGTG AAGCCAAGCC GAAGGAGAAG TTCTTTGAAG AGCTTCCTGT GGTCACAAAG 
GCGCGCCCAG CTTCTCGAAA TACATTGGGC ACCGCATCGC CGGCACAGTC TAGCCCATAC 
GGCCCACCGC CCCCGGCTGG CCCACCTTCT ATCGCCTCGC AGCCACCAGT ACCTGGAATG 
CCGTCTGCCA TGGGATCTGT TCCCAACTCT TCCTTTACCC AGCATGCGGA GGTTCCCAAC 
CTGGTTGCGC CGCCACGTGT CAACCCTTAT GCTTCGATAC AGCCCAGCAC TACGAGCCTT 
GCTCCCGCTG TTCCTGCCCC GGCTTCAAGT CGTTATTCTC CAGCGCCTTC CAGTGGGTCA 
CAGCTCAGCA CTCCCGTCCC ACCTGCTGCG GCAAGCCGTT ACTCTCCCGC CCCGCCAGCT 
TCTCGGCCAA CGAGTTCGGG TTATACTCCG ACTACTTCGG TGCCACCGGT TCTACCACCG 
GTTTTGCCGC ATCAACCCCG TACCTCAAGT CCGCTAGCAC ATTTCGAGTC CAGGCCTCTT 
GTTTCTAGCC ATGCAGAAAG TGGCCTGGCG GAAAAGCGGA GCAGCAGCTC GTTGTACGAC 
TTGCGACTGC AGAGAGTGCC TTCTCTACCT CCGACTCAGG AGGTGGAAGA AGAAGGCCCC 
ATGCAGGGTA TGGTGTCCCC CAACCAAGTC CCGGCATCCC ACCAGACCTC TCCTACGGCG 
TCAAGACATG GCGCTATGCC CCACCGTTCT AGAAGGACAC CGCCTCCGCA GTCTCTAACT 
GTGCAGCCGG TGACGTCCCC TCAGAGGGCG GCATACAGCT ATCAGCCTGC GGCTAGTGCT 
CCATCTCACG AATTCGCGCC ACCACCTCGG TCGCAGACTC AATCCCCTGG TGCCCTCTAC 
GGGAACAGGA ATGTAAAGCC TATTGAACCT ATCCCTCGTC CTTCGTCGGT CACTGACGCG 
ACTTCGCCGC GGGCTGCGTT GTATTCTCCT ATCACCATGC AACCCAGTTC TACTTTCCCC 
CCCATTTCTA CGATTACCTC GCGGCCGCGT GGTAACACTC AGAATTTTAA CCTAGTTCCC 
CCGACAGATG GTAGAGAACA TGATCCCCTA CAGAGATGGC GTGGTGTTCC TCTCATTTCC 
TGGGGCGTAG GTGGAGCTCT GGTAACAATG TTCCCCAAGA ACGTGCCACG ATATGGCATG 
GGCCAAACAG CCCCCATGGT TATTCGTAGC CCAGGAGAAG TCAAGGTCAA GAGCATGAAG 
GACATAGAGC CAATGGAGGA GCGCTTGGCC AAATTCCCTG GGCCTCTAAA GGGGAAGTCG 
AAGAAGAAGG AGACCATTGC CTGGTTAACA AACGGAATTG AGATGCTGGA GAGGACTGTG 
CCACATAACC TGTCTCATCA GCTCAACCCG TCTCACGATG ACAAGCGGAC AACAGAGAGA 
CTCTTGCTTT GGAAGATTCT GAGGGTCTTT GTCGAGCATG ATGGCGTGTT GGAAGGAAAC 
CCGACCGTTA ACCAAGCTGT GCGCGAAATC CTGTACCCGG AGGCTTCAAC CATTGGTGGT 
CAGCCTGAGT TCGCAAATGC TCTGAACCCA TCAGGAATGG GCAATTCTGC CACGACTTCA 
CTACAAGCCG ACTCGGTTGA TTCTTCTGCT GTTGAGCAGA TCCGCAACCA CCTCATCAGT 
GGCGACAATG AGAAGGCAGT GTGGGCTGCG GTCGATAAGC GGCTTTGGGG CCATGCCTTC 
CTGCTGGCCA ACGCCCTTAA CCCTGACCTC TATAAGCGGG TCGCTCAAGA ATTTGTGAAG 
AACGAGGTGA ATTCCACCGG TCATAACAAT GAGTCGCTTG CTGCTCTCTA TGATGTCCTC 
TCTGGCAACC ACGAAGAAAG CGTGGACGAG CTGGTGCCCG CCCATGCTCG AGCTGGTCTT 
CAGCTAGTGG CCAAAAATTC ATCTTCTGGT CCCTCCAAGG ACGCCATGGG TGGTCTTGAT 
AAGTGGCGCG AAACCCTCTC CCTGATCCTC AGCAACAGGA CTGCTGATGA TGCCCGAGCC 
ATCAACTTTT TGGGCAATTT GCTATCAGGC TATGGAAGAG CCGAGGCGGC TCATATTTGC 
TTCTTGTTTG CCAGAAGTCA AACCATCTTC GGCGGCTTGG ACAACCCGAA CTCTAACTTC 
GTTCTTGTTG GGTCTGACCA TAGGCGGCAG GCGGACCATT TCGCAAAGGA GATTGAGCCT 
CTTTTGTTGA GCGAGGTTTA TGAGTACGGC CAGAGCCTTG CTGGTGGTAC CGTGCCCGTC 
AGCAATCCTC ACCTGGCAGC GTACAAGCTT CAGCACGCAT ACGCCCTGGC CGAGTATGGC 
TTTAGAGACA AGGCCCTTCA ATACTGCGAG GCTATCACGG CTGCGATCAC CGCGCAGACG 
AAGCGCTCTC CTTATTACCA TCCCATCCTT GAGGCCTATG TCGATGACTT GATGAAGCGA 
TTGAGGCAGG CACCCAAGGA GGAATCAAAC TCGTGGATCC CGAAGCCTAG CATGAATAAG 
GTATCTGATT CCATGTGGAA TCGGTTCAAC AAGTTTGTCG CTGGAGATGA CAACGAGGAT 
GGCAGTAAGG GCTCACCTGA CGCTGCGGGA GAATCTGGTC CCTTTGCACG AATCGCCGGA 
GGAACCCCGA CTATCAGTCG CTCGCCCTCT GTCAACAATC TTGAGACCTT TGGCGCGACC 
ATCCCCAGCT ATGGCATGCC TAGTGCTCCC GTGACGAACG GTCCTAATAT GTTCTCTCCT 
CCTCCTCCTA CACGGACGGC TTCACGCTAC GCACCAGGCG CTCCTCAACC ATCGACTCCG 
AACTATAACC CCTATGAGAC AAACTCTCCA TATGCGCCTC GCTCATCAAT GGAACGGGCC 
TCAGGCGAGT ACAGCAGAAG TTCAGTTGAG CTTCCGCGTC AGTCTTTGGA CTCTCAGCGG 
GGCTATTCGC ACAGCAGCTA TGCTCCCAAC CGTACATCTT CGCCTGCTCA GCCATATACA 
CCTTACGGCA CGACGCCACA GGAATCATCC TACTCTCTGC ATAACATGCA GCCTCAGCAA 
AGTTTGACAT CCCCAGCGGC TACTACTTCT GGTTATCAAC CCTTCACTCC ACAAAACAAC 
GTCTCGGCAA ATGATGAGCC CTCTAACGAA CCCCCTTCGG CCCCGTCAAC TGGCTACCAG 
CCGCCGTCGT ACGGATATGA GCCTCCGTCA TTCACCCCTT ACGAGGCGCC GGCGACCAAT 
GACGAGGAGG AGGGTGCCTC CAAGGAGAAC GGTGACTCTA ACCAGGGTGG CGAAGGTGTC 
AACACCTTTG AGCCCCCTTC ATTCCAGCCA TATAGCTATG AACCGCCTTC TTACGAACCC 
GACACCCCAC CTTCGAAAGA TGACGATCAA TCTGAGGAAG AAAAGCCCAA GCCGAAGAAG 
AAGGGCCCTA TGTACGATGA CGATGACGAT GATTTCCCCG CGGCGCCGAA GCCTGCCGGC 
AAGTCCAAGG CTGAAATTGA CCGGGAGAAC GAGGAAATGG TCCGTCGGAT CGCCGAAGAA 
GAAGCCAAAC GCGCCGCAGA AGCCAAAGCC GCCAAGAAGG GCTGGGGCTT CACCAGCTGG 
TTCGCAAAGA AGGAGGCTGC CGCCGCCGAT GCCAACGCCG CCGGAAGCAG CCCCGGCAAG 
CCCATCCGTG CCAAGCTCGG CGAGGCCAAC TCGTTCTACT ACGACCCCGA GCAAAAGCGC 
TGGATCAACA AGAACGCCAG TCCCGAAGAC CAAGCCGCCA AGAAATCCAC GCCACCACCA 
CCCAAGGGCG GCATCCCCCG CTCATCCGCC TCCAGCCCTG CTCCTCCCAT GGGTATGGGC 
GTGGGCGGCG GTAGCGCGCC CAACACCCCC GGCCGCGCCA GCGCCCCGCC TACTGGTCCG 
CCTAGACCGG CGGCGCTCAT GCCTTCGGCG TCGGAGAGCA ATGTTGGGTC TGGCCCACCT 
AGCGCTGTTG GTCCGCTGAG TCCCAGTGGA AGTAACGGGC CCCCAAGTGC GGGCTTGCTT 
TCCCCTGGCA TGGGTCCGGC GGCGATGCAG CGCCCGGCCT CGACGAGCAC GAGCGGGCCC 
CCTGCTGGTA CTAGTAAGCC GTTGAGCGCG ACGAGTAGTA TTGATGATTT GTTGGGAGCG 
GCGGTACCGA GGAAGAGGGG TGAGGCGAAG AAGCCGAGGA AGGCGGCTAG GTATGTGGAT 
GTTATGCAGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU03819 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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