NCU08926 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU08926
Entrez Gene ID	3876755
Full Name	ATG2
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_955515.3	XP_960608.2	ATG2

Pathways from BioSystems

Homologies

Neurospora crassa	NCU08926	XP_960608.2
Magnaporthe oryzae (rice blast fungus)	MGG_16734	XP_003711844.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU08926 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU08926 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf06324	XM_955515.3 Latest version!	Neurospora crassa OR74A ATG2 (NCU08926), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU08926 ( XM_955515.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_960608.2 Neurospora crassa OR74A ATG2 (NCU08926), mRNA.

Price & Availability↑

CloneID	ONf06324
Clone ID Related Accession (Same CDS sequence)	XM_955515.3
Accession Version	XM_955515.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	ATG2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026505). On Feb 23, 2015 this sequence version replaced XM_955515.2.

ATGTCTTTCT TTTATCGGTC CTTCGCCAAC TCGCTTCTGC CGAAGCAGTT GCTTCGCTTT 
ATCTTAGCGC GCCTAGACCT CCTCGACTCC CAGGCACTTG ATCTAGATAA CCTCAACTTT 
GCTCTTGGGC GAAATACTGT TTTAGAGTTT CGTGATGTTG GACTTATTCT ATCCAAACTT 
GAAGCCCTCA CAAACCTTCC GCCAACGTTC AAGATCCAAA AGGCCAAAGT TCTCCTACTC 
CGCATCACAA TACCGGTCGA TATCTACAAC AGTCCAATAA TAGTCGAGGT TGAAGGTGTC 
GATACCCGGA TACGGGTGGC GTCCAAGCAA GAGCAGGATG AACAAACGGC CAAAAACAAA 
AAGGGAACAC ACAAGGACGG CGACGAGGTT GTGCCAACTG CTGCCGACCT AGCTCAGTCT 
TTTCTGCAGA CAGAAATCCC GACTGACGAG CAGAGACGGC TGGAAAAGGC TCTTGCCGAG 
GAAGCACAGG AGGCGTTATC AGAGTCCATG AGCGACTCCG AAACCGATGA CGATGGCTCC 
TTCGCCACCT TCGGCACTGG TCAAGGCTTG TCACTACCAA CGTTCTTGAC CAATTTCCTG 
CAGGGCATTT TGGACAGGCT CCAGATCCGA ATCCACAAGG TCACCTTCCA ACTCGACATG 
CAAGTACCCG TCGAACCCGG TCTCTCGACA ATAGAACTTG TTACGTTCCA GCTGGCGTTA 
GACGAGGTCA TTGCGGAAGG AGTCACAGCA CCTGGACAGC AAAAAGACGG TTCGCCTGCG 
ATAGTACCGA GGGAGGGCAA GCGTCATATT CTTCTGGACA ATCTCCGGGC ATTCCTGATC 
ACGGAAGCGA ACGTGTTTTC TTCTCTCCTA CCGACCCAGT CAGCCCAGTC CTTGGTGAAA 
CCACAAGGAC CAGCACCTCA TGATGTACCG TCAACACTTC GTGATATGTC GGGTTCGATG 
CAGTCATCAG TGGGGGGCCT GGATATGAGC ATAAGCCCAG ATCAGATGGA AGCCCTGGAT 
ATGCTTGCAC AGAGTCAATA CGAAGTACGA GATAGCGAAG ATGCTCTGAA TATTCCGTAT 
GAGAGCGACA CTCAGTATCC AGAGGCCGAA GAGAATGTTG CTGGATCTTC GCCTCTCTCA 
ACACCGAGGG CCTCGATGTA CCAAGAACAT GACACGCCGA TGCTCCAGGA CCATGCTAAC 
TCAGCTATCA TGCAACATCA GCCGGAACTT TGGTCATCCT ACGAGCGCGG AATCCGATCA 
GAACCTTCTC TTCATCCTCC AACAAGTTTT CAACCTCAAA CCATGCCCTC TGGTGCTGTG 
TCTCCGGCTC CCTCTGAGCC TTCCTCGTCT GCCAGTTCGG TACGGTCGGA CGACGATACG 
CCAAGCGCTG ATACGGAGGA CCTCGCTCAG TCACATCTCT ACAGTCACGA GGAGGCTGAG 
AGCATGTACA TGAGCGCTTT TTCTGATGCT CATTCGACAC TGATGCCAGG AGCTTATATG 
CCTGGAGGTT GGGGAGCTGA ATCGGAAGGG GGCGAGTCAG CCTCCGAGCG AGACCTCTCA 
ACCACCCATC CAGTCGCAGG TGAACCGCAA TCATCAACCC CTGTTGTCTC GGAACTGGCT 
AGCCCAACAC CTATACCCGA GCAAAACCCA ACAGCCCAAG GTTATGAGCC ACAGCATGAA 
GATACTTCCA CACCACGCGG GATTACGAGG ATGGTAAAGG AGATCGTATC ATTGGACTCC 
ATATCTGTCT ATGTTCCTTC AACGAGAAAG CAGGTTCTGG CACCAGTCAC CAATCTTGCA 
AAGTCAAATC CTAACCTTCC AGGAGCGTTT TCGGTTCACC AATCATTTCA CAGTAGTACT 
ACGGATCAGT CGACGCTTTT AACCCCAGAG AACCTGAAAG AGGATCGCCA GGATTATGCT 
ATCGAAATTG TTCTCAAGCC TCTGACACTC CGGTTCGACT CGTCAGTGGG ATTCTTGCTC 
GCTATGGTTG TTACACGGCT CCTGGAAGCG TTCAAAGGGT CTTCAGACGA GGGGGCAGAA 
GTCAAGCCTT CAAGTACCGA ATCGATTTTA CCTGACATCA AGGTTTGCTT GGAGCAGGTT 
ACGATGCAGT TTCTGGAGGA GCTGACTGGT GTGGCCGACT CTGCAAAGAG GATATACGAG 
ACACAAAAAC CCAATTTCGG CTCAGATGTC CTCCTTGAGG CCAGTCTTAC GAACTTGAAC 
GCCTTTACGC ACCAGTCCGG ATCGCAAACA GAAGTAAACG TCAGCATCGA GAAGTTCGCG 
TTCGGTTATG CCGATGAGGC CATCGTCTCG TTCACTGGCG AGGCTGATTT GTTCCAATCC 
ACAGCAACTG TTGATATGCT ATCTGTTGGG AAGGATATCG CAGTCAAGGC CACCATTACA 
CCAGACGTGA CTCGGGTTGA CGTCAAAACA CTACCGCTCT ACCTAAAGCT CGATTTACAG 
AGGTTAGACG AGACTTTCAG CTGGTTTGGG GGACTGAGCG GTTTCCTCAA TATGGGAGCG 
TCAGAGGACT CCGTAGCAAA GGCAGTACAG ATACCAGCGA AGCCCCCACA AAAGACGCGG 
GGCGTCCGAT TCGAAACCCC GATCGATCCT ATGGAGAAGC GGTCAGGAAG CAAGATTGAT 
GTGCGCATTA ATGGCGTGCA CGTGGACGTG ATTGGCAAAG ACTGCAGGGC AGTCCTGAAC 
ACGAGCGCTC TCAAGCTCGT GCACCGCGAA GAGGCAATTG CCGCTGTGCT GAAAACGATC 
AGGCTCTCCG GGCCCTACGT AAGGAATTCT CATGCTAGAG CGCCAGTCGT ACTAGAGTTG 
GATAGTACAA GGCTGGACTA CATCTTGACA CCTCGGACCA GGGACCTCGA ACGGCTTCTT 
GAGCTGATTA CTCCCTCAAA GGTCAAGTTC GACGAGGACG AAGATGAGAT TATGGTGGAT 
ACTCTGCTAA GGCAGAGGAG GAAGGGGGGA GTTCTTGGGC TAGAGGTTAA GAACTTCAAA 
GTAAGGGCGG GCAACTTGGC TTTACTTGAT TGTATCCCGA GTTTGGTGGA TGATCTCGCC 
AAGCTAGGGA CGGTCGCAAA GTATCTGCCC GAGGATGACA GGCCTGGGCT GCTTACGCTT 
GGGCAGGTCA CAAACGTGGA TTGTGAAGTC GACGTTGGAG GCAGGTTCGG AGTTGTTAAC 
GCGCGTTTGA CAAACCTTGA GCTCGCCCAA ATTACGGTGC CGTCGTTGGT GGCTGTCGCG 
GTAGGCGTTG TTACGGTCAA TCGAAACGAG AACAGCCCGA TCGAGGAGGA GCTGGTTAGC 
ACTTCCACTG CCCATGCTCC GGGTTCTTCA AAGGCGCCCG TCCTGATGAT GCGCATGATC 
GACGATATCG AACCGGTGTT GAAGATCAAG TTGTTCGGGC TGAATGTGGA TTACCGGGTA 
CCGACAATCA TGGACGTGCT GGGGCTCATC CAGGAAGAGA CCACTCCAGA GGAATTTGAG 
GCCAACTTAG CTGCGTCTGT TGCGAACCTC GGAGGCCAGG CGACTGCTGC GCTAAGGAGA 
CAAGATACGC CAGAGAGCCC AGTGGTGGAC AAGGAGTTCA AGCCCATCAA GCTTGATGTA 
GCTTTCCGCG ACTGCGTTGT TGGCCTCAAT CCTCTCGGTC AGGATTCGAA GCTGGCTATC 
GTACTGGCAG ACTCGCATCT GGAAGCCATA CCGGGCAAGG ACAGCACGCT TGACGCTACA 
GCAACCTTGA AGAAGGCGTG CATTCTTCTC ATTGATGATG TGAAAACCCT GCAAGACGTC 
CAGATCAACG CACGAGGACG CGCTCCAGCT ATGAGCACTC CTCAGGCTCT TGAGCTATGC 
AGCAAGGGAT TCGTCAGCAT TTGTGAGATG TCATCTGCAA AAGCCGTCGT CAAGGTTGGC 
AAAGACGAGA ATGGGGAATC CCACGTAGAG GTGTCTGTCC GCGATGACTT GCTTGTTCTC 
GAGACTTGTG CGGACTCAAC GCAGACGCTC ATCTCGTTGG CCAACGCACT CACGCCACCT 
ACTCCCCCTA GCAAGGAAAT CAAGTACAGG ACGTCCGTGT TGCCTGTCGA GGATCTGTTC 
GCATCTATTA CCCCTGACGC ATTCGGAAGA GCGGAGGGCG AGTATGACTT TGACGATGAC 
TTTGCGGGTG CTCAGGGCAT CGAGTGTGGC AATGAAGACG ATGATGACTA CTATGGCATA 
GGAAGCACGG AGCATCTCGA AATCCAGTCA GAGGGGTACG GTGTCGCGGA GGAGTTGTTC 
GATGCCACGA ACAGTTCGCT ACTCGGCGAT ATTGAGGTGG AGAATACGAA TGACGGCATG 
CTGGTTAGCA CTGCAAACCT TGACGTGCCT CCGGTCAGCA GCCAGAGTGA GAGTGACTTG 
GATATTCAGG AGAATTACTT CTCTTCTGAG CCAGTCAAGA ACACAACGCT CCGCTGGAAC 
TCGAGGAAAA ACCTTTACGA TCAGTCAAGT GATGCTCAGG TCTTCAAGAG CCCTCTCGTC 
ATCTGCGTTC GGGATGTTCA TGTCATCTGG AATTTGTATG ATGGTTACGA CTGGGTTCGC 
ACCAGGGAGA TTATCACCAA GGCGGTCCAG GATGTGGAGG CTAAGGCGTA CGAACGTAAA 
GCCCGAGCTG ACCGTCACAC TTATGAGGAT GAAGGAGATG AAGAATCGGT CGTTGATGAC 
TGCTTGTTCA ACTCCATATA TATCGCGGTG GGACCCAACG GTGACCCGAG TAACCTCAGA 
CGGGCTATCA ACCAGGAGTT GCAGTATCAG GACACCACCA CCGAAACCGA GTCCGTCGCC 
ACCACGGCCT TCACCACCTC AACTGTCCGC GCTTCAGGAC ACCGTCAATC GCGGCCGAGG 
GGGAAGACGC TGAAGCTTAG CCGCAGCAAG AACCACAAGA TCACGTTCGA ACTAAAGGGC 
GTCAACATCG ACGTGGTGAC CTTCCCACCA GGCAACGAGA CCATCAATAC CATCGATGTT 
CGCATCCACG ACTTGGACGT CTTTGACCAC ATCCGGACTT CAACATGGAA GAAGTTCGCC 
ATGTACGACA TCGACGCCGG CGAACGGGAG CTTAGTAAGC ACATGGTCCA TCTTGAGGTG 
CTCAACGTCA AGCCTGTCCC GGACTTGCCG GCTACGGAGC TCGTGGTCAA GGTTAACATA 
CTTCCACTGA GACTCCATGT CGACCAGGAC GCTTTGGACT TTATCACGAG GTTCTTTGAG 
TTCAAGGATG AGACGCAGCC TATCCATCAG TCGTCTAGCG ATGTACCATT TATTCAGCGC 
TGCGAGGTCG GTGATGTGCC AGTGCGGTTG GACTTTAAGC CAAAGCGGGT TGACTATGCC 
GGGCTGAGGT CTGGTCACAC GACAGAATTC ATGAACTTTG TCATCCTTGA GGATTCCAGG 
CTGGTGTTGA GACATGTTAT ACTCTATGGC GTCTCCGGTT TTGATAAGTT GGGCAAGAAG 
CTCAACGACA TCTGGACCGC CGACGTCAAG TCGACTCAAC TTCCTGGTGT CCTTGCCGGC 
GTGGCGCCCG TTCGCTCGCT TGTTAATGCC GGATCGGGCT TCAAGGACCT CATTGAGATA 
CCCATCCGGG AGTATAAGAA GGACGGTCGG ATCTTCCGCA GCATCGGTAA AGGAGCTACG 
GCGTTCGCAA AGACAACGGG CACCGAAGTC GTCAAACTCG GTGCCAAACT CGCCATTGGC 
ACTCAATACG CCCTTCAGGG CGCAGAAGGC ATGCTGGCCA AGAACCCACC CAACTACAAT 
CACGCCGGTC CATCCTCTTC CTCCGCCGGC GTACCGGCCG GAGTAGACTA CGAAGTCTGG 
GACGAAGAGG ACTTTGGAGA CCACCATACA CCCAAGGCAA TCAGTCTCTA TGCCGACCAA 
CCGCTAGGCA TCATGCAAGG CATGCGTGGC GCCTACGCCA GCTTATCTCG GGATATAGCG 
ATTGCCAGAG ACGCGATCAT CGCGGTGCCC ACTGAAGTGA TGGAGAGCTC GAGCGCACAG 
GGAGCGGCGA AGGCAGTGCT GATGCAGGCG CCGACGATTT TGTTTAGGCC GGCGATCGGA 
GTGTCCAAGG CGATTGGGCA GACGTTGTTG GGAGCGACGA ATGCGTTGGA TCCGAATCAT 
CGGAAGAGGA TTGAGGCGAA ATACAAGAAG CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_960608.2
CDS	1027..7182
Translation	MSFFYRSFANSLLPKQLLRFILARLDLLDSQALDLDNLNFALGRNTVLEFRDVGLILSKLEALTNLPPTFKIQKAKVLLLRITIPVDIYNSPIIVEVEGVDTRIRVASKQEQDEQTAKNKKGTHKDGDEVVPTAADLAQSFLQTEIPTDEQRRLEKALAEEAQEALSESMSDSETDDDGSFATFGTGQGLSLPTFLTNFLQGILDRLQIRIHKVTFQLDMQVPVEPGLSTIELVTFQLALDEVIAEGVTAPGQQKDGSPAIVPREGKRHILLDNLRAFLITEANVFSSLLPTQSAQSLVKPQGPAPHDVPSTLRDMSGSMQSSVGGLDMSISPDQMEALDMLAQSQYEVRDSEDALNIPYESDTQYPEAEENVAGSSPLSTPRASMYQEHDTPMLQDHANSAIMQHQPELWSSYERGIRSEPSLHPPTSFQPQTMPSGAVSPAPSEPSSSASSVRSDDDTPSADTEDLAQSHLYSHEEAESMYMSAFSDAHSTLMPGAYMPGGWGAESEGGESASERDLSTTHPVAGEPQSSTPVVSELASPTPIPEQNPTAQGYEPQHEDTSTPRGITRMVKEIVSLDSISVYVPSTRKQVLAPVTNLAKSNPNLPGAFSVHQSFHSSTTDQSTLLTPENLKEDRQDYAIEIVLKPLTLRFDSSVGFLLAMVVTRLLEAFKGSSDEGAEVKPSSTESILPDIKVCLEQVTMQFLEELTGVADSAKRIYETQKPNFGSDVLLEASLTNLNAFTHQSGSQTEVNVSIEKFAFGYADEAIVSFTGEADLFQSTATVDMLSVGKDIAVKATITPDVTRVDVKTLPLYLKLDLQRLDETFSWFGGLSGFLNMGASEDSVAKAVQIPAKPPQKTRGVRFETPIDPMEKRSGSKIDVRINGVHVDVIGKDCRAVLNTSALKLVHREEAIAAVLKTIRLSGPYVRNSHARAPVVLELDSTRLDYILTPRTRDLERLLELITPSKVKFDEDEDEIMVDTLLRQRRKGGVLGLEVKNFKVRAGNLALLDCIPSLVDDLAKLGTVAKYLPEDDRPGLLTLGQVTNVDCEVDVGGRFGVVNARLTNLELAQITVPSLVAVAVGVVTVNRNENSPIEEELVSTSTAHAPGSSKAPVLMMRMIDDIEPVLKIKLFGLNVDYRVPTIMDVLGLIQEETTPEEFEANLAASVANLGGQATAALRRQDTPESPVVDKEFKPIKLDVAFRDCVVGLNPLGQDSKLAIVLADSHLEAIPGKDSTLDATATLKKACILLIDDVKTLQDVQINARGRAPAMSTPQALELCSKGFVSICEMSSAKAVVKVGKDENGESHVEVSVRDDLLVLETCADSTQTLISLANALTPPTPPSKEIKYRTSVLPVEDLFASITPDAFGRAEGEYDFDDDFAGAQGIECGNEDDDDYYGIGSTEHLEIQSEGYGVAEELFDATNSSLLGDIEVENTNDGMLVSTANLDVPPVSSQSESDLDIQENYFSSEPVKNTTLRWNSRKNLYDQSSDAQVFKSPLVICVRDVHVIWNLYDGYDWVRTREIITKAVQDVEAKAYERKARADRHTYEDEGDEESVVDDCLFNSIYIAVGPNGDPSNLRRAINQELQYQDTTTETESVATTAFTTSTVRASGHRQSRPRGKTLKLSRSKNHKITFELKGVNIDVVTFPPGNETINTIDVRIHDLDVFDHIRTSTWKKFAMYDIDAGERELSKHMVHLEVLNVKPVPDLPATELVVKVNILPLRLHVDQDALDFITRFFEFKDETQPIHQSSSDVPFIQRCEVGDVPVRLDFKPKRVDYAGLRSGHTTEFMNFVILEDSRLVLRHVILYGVSGFDKLGKKLNDIWTADVKSTQLPGVLAGVAPVRSLVNAGSGFKDLIEIPIREYKKDGRIFRSIGKGATAFAKTTGTEVVKLGAKLAIGTQYALQGAEGMLAKNPPNYNHAGPSSSSAGVPAGVDYEVWDEEDFGDHHTPKAISLYADQPLGIMQGMRGAYASLSRDIAIARDAIIAVPTEVMESSSAQGAAKAVLMQAPTILFRPAIGVSKAIGQTLLGATNALDPNHRKRIEAKYKKH

Target ORF information:

RefSeq Version	XM_955515.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A ATG2 (NCU08926), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_955515.3

ORF Insert Sequence:

ATGTCTTTCT TTTATCGGTC CTTCGCCAAC TCGCTTCTGC CGAAGCAGTT GCTTCGCTTT 
ATCTTAGCGC GCCTAGACCT CCTCGACTCC CAGGCACTTG ATCTAGATAA CCTCAACTTT 
GCTCTTGGGC GAAATACTGT TTTAGAGTTT CGTGATGTTG GACTTATTCT ATCCAAACTT 
GAAGCCCTCA CAAACCTTCC GCCAACGTTC AAGATCCAAA AGGCCAAAGT TCTCCTACTC 
CGCATCACAA TACCGGTCGA TATCTACAAC AGTCCAATAA TAGTCGAGGT TGAAGGTGTC 
GATACCCGGA TACGGGTGGC GTCCAAGCAA GAGCAGGATG AACAAACGGC CAAAAACAAA 
AAGGGAACAC ACAAGGACGG CGACGAGGTT GTGCCAACTG CTGCCGACCT AGCTCAGTCT 
TTTCTGCAGA CAGAAATCCC GACTGACGAG CAGAGACGGC TGGAAAAGGC TCTTGCCGAG 
GAAGCACAGG AGGCGTTATC AGAGTCCATG AGCGACTCCG AAACCGATGA CGATGGCTCC 
TTCGCCACCT TCGGCACTGG TCAAGGCTTG TCACTACCAA CGTTCTTGAC CAATTTCCTG 
CAGGGCATTT TGGACAGGCT CCAGATCCGA ATCCACAAGG TCACCTTCCA ACTCGACATG 
CAAGTACCCG TCGAACCCGG TCTCTCGACA ATAGAACTTG TTACGTTCCA GCTGGCGTTA 
GACGAGGTCA TTGCGGAAGG AGTCACAGCA CCTGGACAGC AAAAAGACGG TTCGCCTGCG 
ATAGTACCGA GGGAGGGCAA GCGTCATATT CTTCTGGACA ATCTCCGGGC ATTCCTGATC 
ACGGAAGCGA ACGTGTTTTC TTCTCTCCTA CCGACCCAGT CAGCCCAGTC CTTGGTGAAA 
CCACAAGGAC CAGCACCTCA TGATGTACCG TCAACACTTC GTGATATGTC GGGTTCGATG 
CAGTCATCAG TGGGGGGCCT GGATATGAGC ATAAGCCCAG ATCAGATGGA AGCCCTGGAT 
ATGCTTGCAC AGAGTCAATA CGAAGTACGA GATAGCGAAG ATGCTCTGAA TATTCCGTAT 
GAGAGCGACA CTCAGTATCC AGAGGCCGAA GAGAATGTTG CTGGATCTTC GCCTCTCTCA 
ACACCGAGGG CCTCGATGTA CCAAGAACAT GACACGCCGA TGCTCCAGGA CCATGCTAAC 
TCAGCTATCA TGCAACATCA GCCGGAACTT TGGTCATCCT ACGAGCGCGG AATCCGATCA 
GAACCTTCTC TTCATCCTCC AACAAGTTTT CAACCTCAAA CCATGCCCTC TGGTGCTGTG 
TCTCCGGCTC CCTCTGAGCC TTCCTCGTCT GCCAGTTCGG TACGGTCGGA CGACGATACG 
CCAAGCGCTG ATACGGAGGA CCTCGCTCAG TCACATCTCT ACAGTCACGA GGAGGCTGAG 
AGCATGTACA TGAGCGCTTT TTCTGATGCT CATTCGACAC TGATGCCAGG AGCTTATATG 
CCTGGAGGTT GGGGAGCTGA ATCGGAAGGG GGCGAGTCAG CCTCCGAGCG AGACCTCTCA 
ACCACCCATC CAGTCGCAGG TGAACCGCAA TCATCAACCC CTGTTGTCTC GGAACTGGCT 
AGCCCAACAC CTATACCCGA GCAAAACCCA ACAGCCCAAG GTTATGAGCC ACAGCATGAA 
GATACTTCCA CACCACGCGG GATTACGAGG ATGGTAAAGG AGATCGTATC ATTGGACTCC 
ATATCTGTCT ATGTTCCTTC AACGAGAAAG CAGGTTCTGG CACCAGTCAC CAATCTTGCA 
AAGTCAAATC CTAACCTTCC AGGAGCGTTT TCGGTTCACC AATCATTTCA CAGTAGTACT 
ACGGATCAGT CGACGCTTTT AACCCCAGAG AACCTGAAAG AGGATCGCCA GGATTATGCT 
ATCGAAATTG TTCTCAAGCC TCTGACACTC CGGTTCGACT CGTCAGTGGG ATTCTTGCTC 
GCTATGGTTG TTACACGGCT CCTGGAAGCG TTCAAAGGGT CTTCAGACGA GGGGGCAGAA 
GTCAAGCCTT CAAGTACCGA ATCGATTTTA CCTGACATCA AGGTTTGCTT GGAGCAGGTT 
ACGATGCAGT TTCTGGAGGA GCTGACTGGT GTGGCCGACT CTGCAAAGAG GATATACGAG 
ACACAAAAAC CCAATTTCGG CTCAGATGTC CTCCTTGAGG CCAGTCTTAC GAACTTGAAC 
GCCTTTACGC ACCAGTCCGG ATCGCAAACA GAAGTAAACG TCAGCATCGA GAAGTTCGCG 
TTCGGTTATG CCGATGAGGC CATCGTCTCG TTCACTGGCG AGGCTGATTT GTTCCAATCC 
ACAGCAACTG TTGATATGCT ATCTGTTGGG AAGGATATCG CAGTCAAGGC CACCATTACA 
CCAGACGTGA CTCGGGTTGA CGTCAAAACA CTACCGCTCT ACCTAAAGCT CGATTTACAG 
AGGTTAGACG AGACTTTCAG CTGGTTTGGG GGACTGAGCG GTTTCCTCAA TATGGGAGCG 
TCAGAGGACT CCGTAGCAAA GGCAGTACAG ATACCAGCGA AGCCCCCACA AAAGACGCGG 
GGCGTCCGAT TCGAAACCCC GATCGATCCT ATGGAGAAGC GGTCAGGAAG CAAGATTGAT 
GTGCGCATTA ATGGCGTGCA CGTGGACGTG ATTGGCAAAG ACTGCAGGGC AGTCCTGAAC 
ACGAGCGCTC TCAAGCTCGT GCACCGCGAA GAGGCAATTG CCGCTGTGCT GAAAACGATC 
AGGCTCTCCG GGCCCTACGT AAGGAATTCT CATGCTAGAG CGCCAGTCGT ACTAGAGTTG 
GATAGTACAA GGCTGGACTA CATCTTGACA CCTCGGACCA GGGACCTCGA ACGGCTTCTT 
GAGCTGATTA CTCCCTCAAA GGTCAAGTTC GACGAGGACG AAGATGAGAT TATGGTGGAT 
ACTCTGCTAA GGCAGAGGAG GAAGGGGGGA GTTCTTGGGC TAGAGGTTAA GAACTTCAAA 
GTAAGGGCGG GCAACTTGGC TTTACTTGAT TGTATCCCGA GTTTGGTGGA TGATCTCGCC 
AAGCTAGGGA CGGTCGCAAA GTATCTGCCC GAGGATGACA GGCCTGGGCT GCTTACGCTT 
GGGCAGGTCA CAAACGTGGA TTGTGAAGTC GACGTTGGAG GCAGGTTCGG AGTTGTTAAC 
GCGCGTTTGA CAAACCTTGA GCTCGCCCAA ATTACGGTGC CGTCGTTGGT GGCTGTCGCG 
GTAGGCGTTG TTACGGTCAA TCGAAACGAG AACAGCCCGA TCGAGGAGGA GCTGGTTAGC 
ACTTCCACTG CCCATGCTCC GGGTTCTTCA AAGGCGCCCG TCCTGATGAT GCGCATGATC 
GACGATATCG AACCGGTGTT GAAGATCAAG TTGTTCGGGC TGAATGTGGA TTACCGGGTA 
CCGACAATCA TGGACGTGCT GGGGCTCATC CAGGAAGAGA CCACTCCAGA GGAATTTGAG 
GCCAACTTAG CTGCGTCTGT TGCGAACCTC GGAGGCCAGG CGACTGCTGC GCTAAGGAGA 
CAAGATACGC CAGAGAGCCC AGTGGTGGAC AAGGAGTTCA AGCCCATCAA GCTTGATGTA 
GCTTTCCGCG ACTGCGTTGT TGGCCTCAAT CCTCTCGGTC AGGATTCGAA GCTGGCTATC 
GTACTGGCAG ACTCGCATCT GGAAGCCATA CCGGGCAAGG ACAGCACGCT TGACGCTACA 
GCAACCTTGA AGAAGGCGTG CATTCTTCTC ATTGATGATG TGAAAACCCT GCAAGACGTC 
CAGATCAACG CACGAGGACG CGCTCCAGCT ATGAGCACTC CTCAGGCTCT TGAGCTATGC 
AGCAAGGGAT TCGTCAGCAT TTGTGAGATG TCATCTGCAA AAGCCGTCGT CAAGGTTGGC 
AAAGACGAGA ATGGGGAATC CCACGTAGAG GTGTCTGTCC GCGATGACTT GCTTGTTCTC 
GAGACTTGTG CGGACTCAAC GCAGACGCTC ATCTCGTTGG CCAACGCACT CACGCCACCT 
ACTCCCCCTA GCAAGGAAAT CAAGTACAGG ACGTCCGTGT TGCCTGTCGA GGATCTGTTC 
GCATCTATTA CCCCTGACGC ATTCGGAAGA GCGGAGGGCG AGTATGACTT TGACGATGAC 
TTTGCGGGTG CTCAGGGCAT CGAGTGTGGC AATGAAGACG ATGATGACTA CTATGGCATA 
GGAAGCACGG AGCATCTCGA AATCCAGTCA GAGGGGTACG GTGTCGCGGA GGAGTTGTTC 
GATGCCACGA ACAGTTCGCT ACTCGGCGAT ATTGAGGTGG AGAATACGAA TGACGGCATG 
CTGGTTAGCA CTGCAAACCT TGACGTGCCT CCGGTCAGCA GCCAGAGTGA GAGTGACTTG 
GATATTCAGG AGAATTACTT CTCTTCTGAG CCAGTCAAGA ACACAACGCT CCGCTGGAAC 
TCGAGGAAAA ACCTTTACGA TCAGTCAAGT GATGCTCAGG TCTTCAAGAG CCCTCTCGTC 
ATCTGCGTTC GGGATGTTCA TGTCATCTGG AATTTGTATG ATGGTTACGA CTGGGTTCGC 
ACCAGGGAGA TTATCACCAA GGCGGTCCAG GATGTGGAGG CTAAGGCGTA CGAACGTAAA 
GCCCGAGCTG ACCGTCACAC TTATGAGGAT GAAGGAGATG AAGAATCGGT CGTTGATGAC 
TGCTTGTTCA ACTCCATATA TATCGCGGTG GGACCCAACG GTGACCCGAG TAACCTCAGA 
CGGGCTATCA ACCAGGAGTT GCAGTATCAG GACACCACCA CCGAAACCGA GTCCGTCGCC 
ACCACGGCCT TCACCACCTC AACTGTCCGC GCTTCAGGAC ACCGTCAATC GCGGCCGAGG 
GGGAAGACGC TGAAGCTTAG CCGCAGCAAG AACCACAAGA TCACGTTCGA ACTAAAGGGC 
GTCAACATCG ACGTGGTGAC CTTCCCACCA GGCAACGAGA CCATCAATAC CATCGATGTT 
CGCATCCACG ACTTGGACGT CTTTGACCAC ATCCGGACTT CAACATGGAA GAAGTTCGCC 
ATGTACGACA TCGACGCCGG CGAACGGGAG CTTAGTAAGC ACATGGTCCA TCTTGAGGTG 
CTCAACGTCA AGCCTGTCCC GGACTTGCCG GCTACGGAGC TCGTGGTCAA GGTTAACATA 
CTTCCACTGA GACTCCATGT CGACCAGGAC GCTTTGGACT TTATCACGAG GTTCTTTGAG 
TTCAAGGATG AGACGCAGCC TATCCATCAG TCGTCTAGCG ATGTACCATT TATTCAGCGC 
TGCGAGGTCG GTGATGTGCC AGTGCGGTTG GACTTTAAGC CAAAGCGGGT TGACTATGCC 
GGGCTGAGGT CTGGTCACAC GACAGAATTC ATGAACTTTG TCATCCTTGA GGATTCCAGG 
CTGGTGTTGA GACATGTTAT ACTCTATGGC GTCTCCGGTT TTGATAAGTT GGGCAAGAAG 
CTCAACGACA TCTGGACCGC CGACGTCAAG TCGACTCAAC TTCCTGGTGT CCTTGCCGGC 
GTGGCGCCCG TTCGCTCGCT TGTTAATGCC GGATCGGGCT TCAAGGACCT CATTGAGATA 
CCCATCCGGG AGTATAAGAA GGACGGTCGG ATCTTCCGCA GCATCGGTAA AGGAGCTACG 
GCGTTCGCAA AGACAACGGG CACCGAAGTC GTCAAACTCG GTGCCAAACT CGCCATTGGC 
ACTCAATACG CCCTTCAGGG CGCAGAAGGC ATGCTGGCCA AGAACCCACC CAACTACAAT 
CACGCCGGTC CATCCTCTTC CTCCGCCGGC GTACCGGCCG GAGTAGACTA CGAAGTCTGG 
GACGAAGAGG ACTTTGGAGA CCACCATACA CCCAAGGCAA TCAGTCTCTA TGCCGACCAA 
CCGCTAGGCA TCATGCAAGG CATGCGTGGC GCCTACGCCA GCTTATCTCG GGATATAGCG 
ATTGCCAGAG ACGCGATCAT CGCGGTGCCC ACTGAAGTGA TGGAGAGCTC GAGCGCACAG 
GGAGCGGCGA AGGCAGTGCT GATGCAGGCG CCGACGATTT TGTTTAGGCC GGCGATCGGA 
GTGTCCAAGG CGATTGGGCA GACGTTGTTG GGAGCGACGA ATGCGTTGGA TCCGAATCAT 
CGGAAGAGGA TTGAGGCGAA ATACAAGAAG CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU08926 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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