lys-5 cDNA ORF clone, Neurospora crassa OR74A

The following lys-5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lys-5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf06214 XM_011396653.1
Latest version!
Neurospora crassa OR74A homocitrate synthase, variant 2 (lys-5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
ONf11427 XM_011396651.1
Latest version!
Neurospora crassa OR74A homocitrate synthase (lys-5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
ONf11427 XM_011396652.1
Latest version!
Neurospora crassa OR74A homocitrate synthase, variant 1 (lys-5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf06214
    Clone ID Related Accession (Same CDS sequence) XM_011396653.1
    Accession Version XM_011396653.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product homocitrate synthase, variant 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGCGCGATG CCCAGCTCGC TGTTGAGACG GGTGTCGACG GCCTCGATGT CGTCATTGGC 
    ACTTCCAGCT TCCTCCGCGA GCACAGCCAC GGCAAGGATA TGGCTTACAT TGAGAAGACC
    GCCGTTGAGG TTATTGAGTA TGTCAAGTCC AAGGGACTTG AGGTTCGCTT CTCCTCCGAG
    GATTCCTTCC GCTCCGATCT CGTCGATCTC CTTTCCCTTT ACCGCGCTGT TGACAAGGTC
    GGCGTCCACC GTGTCGGTAT CGCCGATACC GTCGGCTGCG CTTCTCCCCG CCAGGTCTAT
    GACCTCGTCC GTACCCTTCG CGGCGTCGTT TCGTGCGATA TCGAGACCCA CTTCCACGAC
    GACACTGGCT GCGCCATTGC CAACGCCTAC TGTGCTCTCG AGGCTGGTGC CACCCACATC
    GACACCTCCG TTCTCGGTAT CGGCGAGCGT AACGGTATCA CCCCTCTCGG TGGCTTGATG
    GCTCGCATGA TCGTTACCAG CCCCGACTAC GTCAAGAGCA AGTACAAGCT CCACAAGCTC
    AAGGAGCTCG AGGATTTGGT TGCCGAGGCT GTTGAGATCA ACACCCCCTT CAACAACCCC
    ATCACTGGTT TCTGCGCCTT CACCCACAAG GCTGGCATCC ACGCCAAGGC CATCCTCAAC
    AACCCCAGCA CCTATGAAAT TCTCAACCCT GCCGACTTCG GTCTCACCCG CTACGTCCAC
    TTCGCTTCGC GCTTGACTGG CTGGAACGCC GTCAAGACCC GTGTCGGCCA GCTTGGTCTC
    GAGATGACCG ACGACCAGGT CAAGGAATGT ACCGCCAAGA TCAAGGCCCT TGCCGACGTG
    CGCCCAATCG CCATCGACGA CGCCGATTCG ATCATCCGTA CTTTCCACCT CGGTCTTCAC
    GAGCAGAACA AGGTCCAGCC TCCCGCTGTT GTCGAGAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011394955.1
    CDS446..1387
    Translation

    Target ORF information:

    RefSeq Version XM_011396653.1
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A homocitrate synthase, variant 2 (lys-5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011396653.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGCGCGATG CCCAGCTCGC TGTTGAGACG GGTGTCGACG GCCTCGATGT CGTCATTGGC 
    ACTTCCAGCT TCCTCCGCGA GCACAGCCAC GGCAAGGATA TGGCTTACAT TGAGAAGACC
    GCCGTTGAGG TTATTGAGTA TGTCAAGTCC AAGGGACTTG AGGTTCGCTT CTCCTCCGAG
    GATTCCTTCC GCTCCGATCT CGTCGATCTC CTTTCCCTTT ACCGCGCTGT TGACAAGGTC
    GGCGTCCACC GTGTCGGTAT CGCCGATACC GTCGGCTGCG CTTCTCCCCG CCAGGTCTAT
    GACCTCGTCC GTACCCTTCG CGGCGTCGTT TCGTGCGATA TCGAGACCCA CTTCCACGAC
    GACACTGGCT GCGCCATTGC CAACGCCTAC TGTGCTCTCG AGGCTGGTGC CACCCACATC
    GACACCTCCG TTCTCGGTAT CGGCGAGCGT AACGGTATCA CCCCTCTCGG TGGCTTGATG
    GCTCGCATGA TCGTTACCAG CCCCGACTAC GTCAAGAGCA AGTACAAGCT CCACAAGCTC
    AAGGAGCTCG AGGATTTGGT TGCCGAGGCT GTTGAGATCA ACACCCCCTT CAACAACCCC
    ATCACTGGTT TCTGCGCCTT CACCCACAAG GCTGGCATCC ACGCCAAGGC CATCCTCAAC
    AACCCCAGCA CCTATGAAAT TCTCAACCCT GCCGACTTCG GTCTCACCCG CTACGTCCAC
    TTCGCTTCGC GCTTGACTGG CTGGAACGCC GTCAAGACCC GTGTCGGCCA GCTTGGTCTC
    GAGATGACCG ACGACCAGGT CAAGGAATGT ACCGCCAAGA TCAAGGCCCT TGCCGACGTG
    CGCCCAATCG CCATCGACGA CGCCGATTCG ATCATCCGTA CTTTCCACCT CGGTCTTCAC
    GAGCAGAACA AGGTCCAGCC TCCCGCTGTT GTCGAGAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ONf11427
    Clone ID Related Accession (Same CDS sequence) XM_011396652.1 , XM_011396651.1
    Accession Version XM_011396652.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product homocitrate synthase, variant 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTGCGGAA CCGATGCTCC CAACGGCACC AATGGCGCCT CCAATGGCGT GAACCCCGCC 
    AACTTCCGCT CCAACCCCTA CCAGCCCGTC GGCGACTTCC TCTCCAATGT CGGCAGCTTC
    AAGATTATCG AGTCCACTCT TCGCGAGGGT GAGCAATTCG CCAACGCCTA CTTCGACACC
    GAGACCAAGA TCAAGATTGC CAAGGCCCTT GACGACTTTG GCGTCGATTA CATCGAGTTG
    ACCTCTCCTG CTGCTTCGGA GCAGTCTCGG AGAGATTGCG AGGCCATCTG CAAGCTCGGC
    CTCAAGGCCA AGATCTTGAC TCACGTCCGC TGCGATATGC GCGATGCCCA GCTCGCTGTT
    GAGACGGGTG TCGACGGCCT CGATGTCGTC ATTGGCACTT CCAGCTTCCT CCGCGAGCAC
    AGCCACGGCA AGGATATGGC TTACATTGAG AAGACCGCCG TTGAGGTTAT TGAGTATGTC
    AAGTCCAAGG GACTTGAGGT TCGCTTCTCC TCCGAGGATT CCTTCCGCTC CGATCTCGTC
    GATCTCCTTT CCCTTTACCG CGCTGTTGAC AAGGTCGGCG TCCACCGTGT CGGTATCGCC
    GATACCGTCG GCTGCGCTTC TCCCCGCCAG GTCTATGACC TCGTCCGTAC CCTTCGCGGC
    GTCGTTTCGT GCGATATCGA GACCCACTTC CACGACGACA CTGGCTGCGC CATTGCCAAC
    GCCTACTGTG CTCTCGAGGC TGGTGCCACC CACATCGACA CCTCCGTTCT CGGTATCGGC
    GAGCGTAACG GTATCACCCC TCTCGGTGGC TTGATGGCTC GCATGATCGT TACCAGCCCC
    GACTACGTCA AGAGCAAGTA CAAGCTCCAC AAGCTCAAGG AGCTCGAGGA TTTGGTTGCC
    GAGGCTGTTG AGATCAACAC CCCCTTCAAC AACCCCATCA CTGGTTTCTG CGCCTTCACC
    CACAAGGCTG GCATCCACGC CAAGGCCATC CTCAACAACC CCAGCACCTA TGAAATTCTC
    AACCCTGCCG ACTTCGGTCT CACCCGCTAC GTCCACTTCG CTTCGCGCTT GACTGGCTGG
    AACGCCGTCA AGACCCGTGT CGGCCAGCTT GGTCTCGAGA TGACCGACGA CCAGGTCAAG
    GAATGTACCG CCAAGATCAA GGCCCTTGCC GACGTGCGCC CAATCGCCAT CGACGACGCC
    GATTCGATCA TCCGTACTTT CCACCTCGGT CTTCACGAGC AGAACAAGGT CCAGCCTCCC
    GCTGTTGTCG AGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011394954.1
    CDS256..1533
    Translation

    Target ORF information:

    RefSeq Version XM_011396652.1
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A homocitrate synthase, variant 1 (lys-5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011396652.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTGCGGAA CCGATGCTCC CAACGGCACC AATGGCGCCT CCAATGGCGT GAACCCCGCC 
    AACTTCCGCT CCAACCCCTA CCAGCCCGTC GGCGACTTCC TCTCCAATGT CGGCAGCTTC
    AAGATTATCG AGTCCACTCT TCGCGAGGGT GAGCAATTCG CCAACGCCTA CTTCGACACC
    GAGACCAAGA TCAAGATTGC CAAGGCCCTT GACGACTTTG GCGTCGATTA CATCGAGTTG
    ACCTCTCCTG CTGCTTCGGA GCAGTCTCGG AGAGATTGCG AGGCCATCTG CAAGCTCGGC
    CTCAAGGCCA AGATCTTGAC TCACGTCCGC TGCGATATGC GCGATGCCCA GCTCGCTGTT
    GAGACGGGTG TCGACGGCCT CGATGTCGTC ATTGGCACTT CCAGCTTCCT CCGCGAGCAC
    AGCCACGGCA AGGATATGGC TTACATTGAG AAGACCGCCG TTGAGGTTAT TGAGTATGTC
    AAGTCCAAGG GACTTGAGGT TCGCTTCTCC TCCGAGGATT CCTTCCGCTC CGATCTCGTC
    GATCTCCTTT CCCTTTACCG CGCTGTTGAC AAGGTCGGCG TCCACCGTGT CGGTATCGCC
    GATACCGTCG GCTGCGCTTC TCCCCGCCAG GTCTATGACC TCGTCCGTAC CCTTCGCGGC
    GTCGTTTCGT GCGATATCGA GACCCACTTC CACGACGACA CTGGCTGCGC CATTGCCAAC
    GCCTACTGTG CTCTCGAGGC TGGTGCCACC CACATCGACA CCTCCGTTCT CGGTATCGGC
    GAGCGTAACG GTATCACCCC TCTCGGTGGC TTGATGGCTC GCATGATCGT TACCAGCCCC
    GACTACGTCA AGAGCAAGTA CAAGCTCCAC AAGCTCAAGG AGCTCGAGGA TTTGGTTGCC
    GAGGCTGTTG AGATCAACAC CCCCTTCAAC AACCCCATCA CTGGTTTCTG CGCCTTCACC
    CACAAGGCTG GCATCCACGC CAAGGCCATC CTCAACAACC CCAGCACCTA TGAAATTCTC
    AACCCTGCCG ACTTCGGTCT CACCCGCTAC GTCCACTTCG CTTCGCGCTT GACTGGCTGG
    AACGCCGTCA AGACCCGTGT CGGCCAGCTT GGTCTCGAGA TGACCGACGA CCAGGTCAAG
    GAATGTACCG CCAAGATCAA GGCCCTTGCC GACGTGCGCC CAATCGCCAT CGACGACGCC
    GATTCGATCA TCCGTACTTT CCACCTCGGT CTTCACGAGC AGAACAAGGT CCAGCCTCCC
    GCTGTTGTCG AGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ONf11427
    Clone ID Related Accession (Same CDS sequence) XM_011396652.1 , XM_011396651.1
    Accession Version XM_011396651.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product homocitrate synthase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTGCGGAA CCGATGCTCC CAACGGCACC AATGGCGCCT CCAATGGCGT GAACCCCGCC 
    AACTTCCGCT CCAACCCCTA CCAGCCCGTC GGCGACTTCC TCTCCAATGT CGGCAGCTTC
    AAGATTATCG AGTCCACTCT TCGCGAGGGT GAGCAATTCG CCAACGCCTA CTTCGACACC
    GAGACCAAGA TCAAGATTGC CAAGGCCCTT GACGACTTTG GCGTCGATTA CATCGAGTTG
    ACCTCTCCTG CTGCTTCGGA GCAGTCTCGG AGAGATTGCG AGGCCATCTG CAAGCTCGGC
    CTCAAGGCCA AGATCTTGAC TCACGTCCGC TGCGATATGC GCGATGCCCA GCTCGCTGTT
    GAGACGGGTG TCGACGGCCT CGATGTCGTC ATTGGCACTT CCAGCTTCCT CCGCGAGCAC
    AGCCACGGCA AGGATATGGC TTACATTGAG AAGACCGCCG TTGAGGTTAT TGAGTATGTC
    AAGTCCAAGG GACTTGAGGT TCGCTTCTCC TCCGAGGATT CCTTCCGCTC CGATCTCGTC
    GATCTCCTTT CCCTTTACCG CGCTGTTGAC AAGGTCGGCG TCCACCGTGT CGGTATCGCC
    GATACCGTCG GCTGCGCTTC TCCCCGCCAG GTCTATGACC TCGTCCGTAC CCTTCGCGGC
    GTCGTTTCGT GCGATATCGA GACCCACTTC CACGACGACA CTGGCTGCGC CATTGCCAAC
    GCCTACTGTG CTCTCGAGGC TGGTGCCACC CACATCGACA CCTCCGTTCT CGGTATCGGC
    GAGCGTAACG GTATCACCCC TCTCGGTGGC TTGATGGCTC GCATGATCGT TACCAGCCCC
    GACTACGTCA AGAGCAAGTA CAAGCTCCAC AAGCTCAAGG AGCTCGAGGA TTTGGTTGCC
    GAGGCTGTTG AGATCAACAC CCCCTTCAAC AACCCCATCA CTGGTTTCTG CGCCTTCACC
    CACAAGGCTG GCATCCACGC CAAGGCCATC CTCAACAACC CCAGCACCTA TGAAATTCTC
    AACCCTGCCG ACTTCGGTCT CACCCGCTAC GTCCACTTCG CTTCGCGCTT GACTGGCTGG
    AACGCCGTCA AGACCCGTGT CGGCCAGCTT GGTCTCGAGA TGACCGACGA CCAGGTCAAG
    GAATGTACCG CCAAGATCAA GGCCCTTGCC GACGTGCGCC CAATCGCCAT CGACGACGCC
    GATTCGATCA TCCGTACTTT CCACCTCGGT CTTCACGAGC AGAACAAGGT CCAGCCTCCC
    GCTGTTGTCG AGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011394953.1
    CDS621..1898
    Translation

    Target ORF information:

    RefSeq Version XM_011396651.1
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A homocitrate synthase (lys-5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011396651.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTGCGGAA CCGATGCTCC CAACGGCACC AATGGCGCCT CCAATGGCGT GAACCCCGCC 
    AACTTCCGCT CCAACCCCTA CCAGCCCGTC GGCGACTTCC TCTCCAATGT CGGCAGCTTC
    AAGATTATCG AGTCCACTCT TCGCGAGGGT GAGCAATTCG CCAACGCCTA CTTCGACACC
    GAGACCAAGA TCAAGATTGC CAAGGCCCTT GACGACTTTG GCGTCGATTA CATCGAGTTG
    ACCTCTCCTG CTGCTTCGGA GCAGTCTCGG AGAGATTGCG AGGCCATCTG CAAGCTCGGC
    CTCAAGGCCA AGATCTTGAC TCACGTCCGC TGCGATATGC GCGATGCCCA GCTCGCTGTT
    GAGACGGGTG TCGACGGCCT CGATGTCGTC ATTGGCACTT CCAGCTTCCT CCGCGAGCAC
    AGCCACGGCA AGGATATGGC TTACATTGAG AAGACCGCCG TTGAGGTTAT TGAGTATGTC
    AAGTCCAAGG GACTTGAGGT TCGCTTCTCC TCCGAGGATT CCTTCCGCTC CGATCTCGTC
    GATCTCCTTT CCCTTTACCG CGCTGTTGAC AAGGTCGGCG TCCACCGTGT CGGTATCGCC
    GATACCGTCG GCTGCGCTTC TCCCCGCCAG GTCTATGACC TCGTCCGTAC CCTTCGCGGC
    GTCGTTTCGT GCGATATCGA GACCCACTTC CACGACGACA CTGGCTGCGC CATTGCCAAC
    GCCTACTGTG CTCTCGAGGC TGGTGCCACC CACATCGACA CCTCCGTTCT CGGTATCGGC
    GAGCGTAACG GTATCACCCC TCTCGGTGGC TTGATGGCTC GCATGATCGT TACCAGCCCC
    GACTACGTCA AGAGCAAGTA CAAGCTCCAC AAGCTCAAGG AGCTCGAGGA TTTGGTTGCC
    GAGGCTGTTG AGATCAACAC CCCCTTCAAC AACCCCATCA CTGGTTTCTG CGCCTTCACC
    CACAAGGCTG GCATCCACGC CAAGGCCATC CTCAACAACC CCAGCACCTA TGAAATTCTC
    AACCCTGCCG ACTTCGGTCT CACCCGCTAC GTCCACTTCG CTTCGCGCTT GACTGGCTGG
    AACGCCGTCA AGACCCGTGT CGGCCAGCTT GGTCTCGAGA TGACCGACGA CCAGGTCAAG
    GAATGTACCG CCAAGATCAA GGCCCTTGCC GACGTGCGCC CAATCGCCAT CGACGACGCC
    GATTCGATCA TCCGTACTTT CCACCTCGGT CTTCACGAGC AGAACAAGGT CCAGCCTCCC
    GCTGTTGTCG AGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B