frq cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	frq
Entrez Gene ID	3876095
Full Name	frequency
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011396823.1	XP_011395125.1	period clock protein FRQ
XM_011396824.1	XP_011395126.1	period clock protein FRQ, variant 1
XM_011396822.1	XP_011395124.1	period clock protein FRQ, variant 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Altogether, our data indicate that the INO80 complex suppresses frq transcription by re-assembling the suppressive mechanisms at the frq promoter after transcription of frq.
Title: Transcriptional repression of frequency by the IEC-1-INO80 complex is required for normal Neurospora circadian clock function.

loss of histone H3K36 methylation, due to either deletion of SET-2 or H3K36R mutation, results in arrhythmic frq transcription and loss of overt circadian clock rhythmicity
Title: Suppression of WHITE COLLAR-independent frequency Transcription by Histone H3 Lysine 36 Methyltransferase SET-2 Is Necessary for Clock Function in Neurospora.

This review focus is on the recent progress in understanding the molecular network governing the expression and activity of FRQ. [review]
Title: Mechanism of the Neurospora circadian clock, a FREQUENCY-centric view.

The affinity of CK1a for FRQ decreases with increasing FRQ phosphorylation, suggesting functional inactivation of FRQ in the negative feedback loop of the circadian clock before and independent of its degradation.
Title: The RNA helicase FRH is an ATP-dependent regulator of CK1a in the circadian clock of Neurospora crassa.

Data indicate the relationship of the light responsive elements of the frq promoter to its circadian feedback components.
Title: A kinetic study of the effects of light on circadian rhythmicity of the frq promoter of Neurospora crassa.

The following frq gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the frq cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONf07679	XM_011396822.1 Latest version!	Neurospora crassa OR74A period clock protein FRQ, variant 2 (frq), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ONf11240	XM_011396824.1 Latest version!	Neurospora crassa OR74A period clock protein FRQ, variant 1 (frq), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ONf11240	XM_011396823.1 Latest version!	Neurospora crassa OR74A period clock protein FRQ (frq), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

frq ( XM_011396822.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011395124.1 Neurospora crassa OR74A period clock protein FRQ, variant 2 (frq), mRNA.

Price & Availability↑

CloneID	ONf07679
Clone ID Related Accession (Same CDS sequence)	XM_011396822.1
Accession Version	XM_011396822.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	period clock protein FRQ, variant 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507).

ATGGAAGTCG ATCCTCCTTT TTATCAAAAG GAGACTGACT CCTCCAACGA GGAATCCAGG 
TATCCGCCTG GACGAAACCC TGTACATCCG CCCGGTGGTG TCCAGTTACC CGGGTTCCGC 
CCGGTTGCTG CTCACAGCAG CGCCGCCGAT GACTACCGCA GTGTCATTGA CGACTTGACT 
GTCGAAAACA AGCGGCTTAA GGAGGAACTG AAGAGGTATA AGCAGTTTGG TTCCGACGTG 
ATGAGGAAAG AGAAGTTGTT TGAGATCAAG GTACATGGCT TACCTAGAAG AAAGAAACGT 
GAGTTGGAGG CAACGCTCCG CGACTTTGCT GCCAGTCTCG GGGACTCGAG CGAATCGACA 
TCGCAGAGGA GAAAGACTGG CAGACATGGA ACGGCCGTTC ACTCGTCTGG GGTTTCGTTG 
TCGAAACACG ATTCGTCGTC ATCGTCGCGC AGTCGTCCTG TCGATTCTGC ATACAACTCT 
ATGTCGACGG GCCGTAGCTC TCATGCGCCC CACTCTTCCG GTCCGTCGCT CGGACGACCT 
TCGCTGACCA GGGCAAAATC CGTCGGGACC CAAAAGGTAG AAAACTATCT GCGCGACACG 
CCCGATGGCC TGCTTCCTCA CCATATTGTC ATGACCGACA AGGAGAAGAA GAAGCTGGTT 
GTCCGACGCT TGGAGCAACT GTTCACCGGA AAGATCAGTG GACGAAACAT GCAGCGCAAC 
CAGTCGATGC CGAGCATGGA TGCACCACTC GCGCCGGAAG GAACCAACAT GGCCCCCCCT 
CGCCCCCCTC CGGAAGGATT GCGCGAAGCT TGCATCCAAC TACAGGATGG AGACAACCCG 
CGAAAGAACC GTTCCTCCAA AGACAATGGT TCTGCATCCA ACTCGGGTGG CGATCAAACC 
GAACTTGGCG GAACTGGGAC TGGAAGCGGA GATGGAAGTG GAAGCGGCGG TCGCACTGGT 
AACAACACCT CGCCCCCGGG AGCTATTGCG CCGGACCAAC GACCAACCAG GCCTCGCGAT 
TTGGACCCGG ACCGCGTCCA GATACCATCC GAGAATATGG ACTACATCCG TCATCTGGGC 
CTTGTATCTC CCGAATTCTT GCAGGGAAGC CGGACCAGCT ATCAAGATGT TGCTCCGGAT 
GCGGAAGGCT GGGTGTATCT CAATCTGCTT TGTAACCTGG CCCAGCTGCA CATGGTTAAC 
GTAACGCCGA GTTTTATTCG CCAAGCTGTC TCCGAAAAGA GCACAAAGTT CCAGCTGTCT 
GCTGATGGTC GGAAAATCCG CTGGCGTGGC GGAACCGATG GAACCAAGTT CAGCAGCGAC 
AGCAGCGAGG ACAAGTCCCA GCAAAGCCCT ATGACCGAAG ATACCGAGGA TGGCTCGGAT 
AAGAATGGTC GGAGGAAGAA GCGGAAAACG CAGCAGGCGA GTTCGGAGAT TGGGAGGTTC 
GGTCCCTCAA GGTCACCTTC GGACACCTTT CATTACAAAC CGATGTTTGT ACACCGTAAT 
TCATCCTCAA TCGAGACCTC TTTGGAGGAG TCGATGTCAC AAGGCTCAGA GGATGCTGTC 
GATGAGAGCA ACATGGGTAA CTCGAAGTGG GATTTTAGCG GATCGGGGAC AACGCAACAA 
CGGAGGAAGC GTCGATACGA TGGCGCCATC GTTTACTACA CCGGCGCTCC ATTTTGCACC 
GATCTTTCAG GAGACCCTGG CGATATGTCG CCAACAGCGC AAATGACGGC CGGCCGCGAG 
GTCGAAGGAT CCGGATCTGG TGATGAAGTT GAACATGTGC TGCAGCGGAC GTTGTCTGGA 
TCGTCTCTTC CTATTAGGCC GCTCAGTGAT GACCGGGCCC GCGTGGCCGA GGTGTTGGAT 
TTCGACCCCG GAAACCCGCC TGAACTCGTG GCCGACGACG GTTCCTCGCC GAATGACGAG 
GATTTCGTCT TCCCATGGTG CGAGGATCCT GCCAAGGTTC GGATTCAGCC TATTGCCAAA 
GAAGTCATGG AGCCCTCTGG TCTTGGTGGA GTACTTCCTG ACGACCATTT TGTGATGCTC 
GTCACCACTC GCCGCGTCGT CAGACCTATC CTGCAGCGGC AGTTGTCACG CTCGACAACA 
TCGGAAGATA CGGCCGAATT CATCGCCGAA CGCTTGGCAG CGATAAGAAC GTCGTCGCCT 
CTGCCTCCTC GCAGTCACAG GCTGACTGTC GCGCCGTTGC AGGTTGAATA CGTCTCTGGG 
CAATTCCGTC GGCTGAATCC AGCCCCACTC CCTCCTCCGG CGATTTTCTA TCCCCCGTTC 
AGTACCGATT CCAGCTGGGA CGATGGCGAT GATCTAGCTT CTGACGATGA AGAAGTGGAG 
GAGGTGGAGG AAGATTCTTA CTCTGAGGGG CAGATCTCGC GCCGAGCAAA TCCTCATTTC 
TCGGACAACA ACACGTACAT GCGGAAGGAC GATTTGGCGT TCGATACCGA GACTGATGTG 
CGGATGGACA GCGACGACAA TCGCTTGTCG GACAGCGGCC ACAACATGCG GGCGATGATG 
CCGCGGGCAG AAGCGGTGGA TGGGGACGAC AGCCCATTGG CAGCCGTCAC CGGAAAAGAG 
GTGGACATGC TGCACACTGG CAGCTCGGTT GCAACCGCTG GTGGGGCAGA GAGTGGCTAT 
AGTAGTTCGA TGGAGGACGT CTCATCCTCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011395124.1
CDS	1011..3683
Translation	MEVDPPFYQKETDSSNEESRYPPGRNPVHPPGGVQLPGFRPVAAHSSAADDYRSVIDDLTVENKRLKEELKRYKQFGSDVMRKEKLFEIKVHGLPRRKKRELEATLRDFAASLGDSSESTSQRRKTGRHGTAVHSSGVSLSKHDSSSSSRSRPVDSAYNSMSTGRSSHAPHSSGPSLGRPSLTRAKSVGTQKVENYLRDTPDGLLPHHIVMTDKEKKKLVVRRLEQLFTGKISGRNMQRNQSMPSMDAPLAPEGTNMAPPRPPPEGLREACIQLQDGDNPRKNRSSKDNGSASNSGGDQTELGGTGTGSGDGSGSGGRTGNNTSPPGAIAPDQRPTRPRDLDPDRVQIPSENMDYIRHLGLVSPEFLQGSRTSYQDVAPDAEGWVYLNLLCNLAQLHMVNVTPSFIRQAVSEKSTKFQLSADGRKIRWRGGTDGTKFSSDSSEDKSQQSPMTEDTEDGSDKNGRRKKRKTQQASSEIGRFGPSRSPSDTFHYKPMFVHRNSSSIETSLEESMSQGSEDAVDESNMGNSKWDFSGSGTTQQRRKRRYDGAIVYYTGAPFCTDLSGDPGDMSPTAQMTAGREVEGSGSGDEVEHVLQRTLSGSSLPIRPLSDDRARVAEVLDFDPGNPPELVADDGSSPNDEDFVFPWCEDPAKVRIQPIAKEVMEPSGLGGVLPDDHFVMLVTTRRVVRPILQRQLSRSTTSEDTAEFIAERLAAIRTSSPLPPRSHRLTVAPLQVEYVSGQFRRLNPAPLPPPAIFYPPFSTDSSWDDGDDLASDDEEVEEVEEDSYSEGQISRRANPHFSDNNTYMRKDDLAFDTETDVRMDSDDNRLSDSGHNMRAMMPRAEAVDGDDSPLAAVTGKEVDMLHTGSSVATAGGAESGYSSSMEDVSSS

Target ORF information:

RefSeq Version	XM_011396822.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A period clock protein FRQ, variant 2 (frq), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396822.1

ORF Insert Sequence:

ATGGAAGTCG ATCCTCCTTT TTATCAAAAG GAGACTGACT CCTCCAACGA GGAATCCAGG 
TATCCGCCTG GACGAAACCC TGTACATCCG CCCGGTGGTG TCCAGTTACC CGGGTTCCGC 
CCGGTTGCTG CTCACAGCAG CGCCGCCGAT GACTACCGCA GTGTCATTGA CGACTTGACT 
GTCGAAAACA AGCGGCTTAA GGAGGAACTG AAGAGGTATA AGCAGTTTGG TTCCGACGTG 
ATGAGGAAAG AGAAGTTGTT TGAGATCAAG GTACATGGCT TACCTAGAAG AAAGAAACGT 
GAGTTGGAGG CAACGCTCCG CGACTTTGCT GCCAGTCTCG GGGACTCGAG CGAATCGACA 
TCGCAGAGGA GAAAGACTGG CAGACATGGA ACGGCCGTTC ACTCGTCTGG GGTTTCGTTG 
TCGAAACACG ATTCGTCGTC ATCGTCGCGC AGTCGTCCTG TCGATTCTGC ATACAACTCT 
ATGTCGACGG GCCGTAGCTC TCATGCGCCC CACTCTTCCG GTCCGTCGCT CGGACGACCT 
TCGCTGACCA GGGCAAAATC CGTCGGGACC CAAAAGGTAG AAAACTATCT GCGCGACACG 
CCCGATGGCC TGCTTCCTCA CCATATTGTC ATGACCGACA AGGAGAAGAA GAAGCTGGTT 
GTCCGACGCT TGGAGCAACT GTTCACCGGA AAGATCAGTG GACGAAACAT GCAGCGCAAC 
CAGTCGATGC CGAGCATGGA TGCACCACTC GCGCCGGAAG GAACCAACAT GGCCCCCCCT 
CGCCCCCCTC CGGAAGGATT GCGCGAAGCT TGCATCCAAC TACAGGATGG AGACAACCCG 
CGAAAGAACC GTTCCTCCAA AGACAATGGT TCTGCATCCA ACTCGGGTGG CGATCAAACC 
GAACTTGGCG GAACTGGGAC TGGAAGCGGA GATGGAAGTG GAAGCGGCGG TCGCACTGGT 
AACAACACCT CGCCCCCGGG AGCTATTGCG CCGGACCAAC GACCAACCAG GCCTCGCGAT 
TTGGACCCGG ACCGCGTCCA GATACCATCC GAGAATATGG ACTACATCCG TCATCTGGGC 
CTTGTATCTC CCGAATTCTT GCAGGGAAGC CGGACCAGCT ATCAAGATGT TGCTCCGGAT 
GCGGAAGGCT GGGTGTATCT CAATCTGCTT TGTAACCTGG CCCAGCTGCA CATGGTTAAC 
GTAACGCCGA GTTTTATTCG CCAAGCTGTC TCCGAAAAGA GCACAAAGTT CCAGCTGTCT 
GCTGATGGTC GGAAAATCCG CTGGCGTGGC GGAACCGATG GAACCAAGTT CAGCAGCGAC 
AGCAGCGAGG ACAAGTCCCA GCAAAGCCCT ATGACCGAAG ATACCGAGGA TGGCTCGGAT 
AAGAATGGTC GGAGGAAGAA GCGGAAAACG CAGCAGGCGA GTTCGGAGAT TGGGAGGTTC 
GGTCCCTCAA GGTCACCTTC GGACACCTTT CATTACAAAC CGATGTTTGT ACACCGTAAT 
TCATCCTCAA TCGAGACCTC TTTGGAGGAG TCGATGTCAC AAGGCTCAGA GGATGCTGTC 
GATGAGAGCA ACATGGGTAA CTCGAAGTGG GATTTTAGCG GATCGGGGAC AACGCAACAA 
CGGAGGAAGC GTCGATACGA TGGCGCCATC GTTTACTACA CCGGCGCTCC ATTTTGCACC 
GATCTTTCAG GAGACCCTGG CGATATGTCG CCAACAGCGC AAATGACGGC CGGCCGCGAG 
GTCGAAGGAT CCGGATCTGG TGATGAAGTT GAACATGTGC TGCAGCGGAC GTTGTCTGGA 
TCGTCTCTTC CTATTAGGCC GCTCAGTGAT GACCGGGCCC GCGTGGCCGA GGTGTTGGAT 
TTCGACCCCG GAAACCCGCC TGAACTCGTG GCCGACGACG GTTCCTCGCC GAATGACGAG 
GATTTCGTCT TCCCATGGTG CGAGGATCCT GCCAAGGTTC GGATTCAGCC TATTGCCAAA 
GAAGTCATGG AGCCCTCTGG TCTTGGTGGA GTACTTCCTG ACGACCATTT TGTGATGCTC 
GTCACCACTC GCCGCGTCGT CAGACCTATC CTGCAGCGGC AGTTGTCACG CTCGACAACA 
TCGGAAGATA CGGCCGAATT CATCGCCGAA CGCTTGGCAG CGATAAGAAC GTCGTCGCCT 
CTGCCTCCTC GCAGTCACAG GCTGACTGTC GCGCCGTTGC AGGTTGAATA CGTCTCTGGG 
CAATTCCGTC GGCTGAATCC AGCCCCACTC CCTCCTCCGG CGATTTTCTA TCCCCCGTTC 
AGTACCGATT CCAGCTGGGA CGATGGCGAT GATCTAGCTT CTGACGATGA AGAAGTGGAG 
GAGGTGGAGG AAGATTCTTA CTCTGAGGGG CAGATCTCGC GCCGAGCAAA TCCTCATTTC 
TCGGACAACA ACACGTACAT GCGGAAGGAC GATTTGGCGT TCGATACCGA GACTGATGTG 
CGGATGGACA GCGACGACAA TCGCTTGTCG GACAGCGGCC ACAACATGCG GGCGATGATG 
CCGCGGGCAG AAGCGGTGGA TGGGGACGAC AGCCCATTGG CAGCCGTCAC CGGAAAAGAG 
GTGGACATGC TGCACACTGG CAGCTCGGTT GCAACCGCTG GTGGGGCAGA GAGTGGCTAT 
AGTAGTTCGA TGGAGGACGT CTCATCCTCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frq ( XM_011396823.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011395125.1 Neurospora crassa OR74A period clock protein FRQ (frq), mRNA.

Price & Availability↑

CloneID	ONf11240
Clone ID Related Accession (Same CDS sequence)	XM_011396823.1 , XM_011396824.1
Accession Version	XM_011396823.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	period clock protein FRQ
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507).

ATGGCGGATA GTGGGGATAA ATCCCAGGGC ATGCGCCCAC CACCCTTTGA CAGCCGTGGT 
CACCCTCTTC CCCGACGTGC TTCCCCAGAC AAATCAATAA CACTTGAAAA TCACCGCCTT 
GCGCGAGATA CTAGCAGCAG AGTTACTTCA TCTTCCGCAC TGGGAGTGAC CGAATCCCAA 
CCCCAACTCA AGAGCTCGCC CACCCGAAGG AATTCGTCTG GAGAGAGCGA GCCCACGAAT 
TGGTTTAATC AATCCAATCG TAACCCTGCC GCGGCCTTTC ATGACGAGAG CCATATCATG 
GAAGTCGATC CTCCTTTTTA TCAAAAGGAG ACTGACTCCT CCAACGAGGA ATCCAGGTAT 
CCGCCTGGAC GAAACCCTGT ACATCCGCCC GGTGGTGTCC AGTTACCCGG GTTCCGCCCG 
GTTGCTGCTC ACAGCAGCGC CGCCGATGAC TACCGCAGTG TCATTGACGA CTTGACTGTC 
GAAAACAAGC GGCTTAAGGA GGAACTGAAG AGGTATAAGC AGTTTGGTTC CGACGTGATG 
AGGAAAGAGA AGTTGTTTGA GATCAAGGTA CATGGCTTAC CTAGAAGAAA GAAACGTGAG 
TTGGAGGCAA CGCTCCGCGA CTTTGCTGCC AGTCTCGGGG ACTCGAGCGA ATCGACATCG 
CAGAGGAGAA AGACTGGCAG ACATGGAACG GCCGTTCACT CGTCTGGGGT TTCGTTGTCG 
AAACACGATT CGTCGTCATC GTCGCGCAGT CGTCCTGTCG ATTCTGCATA CAACTCTATG 
TCGACGGGCC GTAGCTCTCA TGCGCCCCAC TCTTCCGGTC CGTCGCTCGG ACGACCTTCG 
CTGACCAGGG CAAAATCCGT CGGGACCCAA AAGGTAGAAA ACTATCTGCG CGACACGCCC 
GATGGCCTGC TTCCTCACCA TATTGTCATG ACCGACAAGG AGAAGAAGAA GCTGGTTGTC 
CGACGCTTGG AGCAACTGTT CACCGGAAAG ATCAGTGGAC GAAACATGCA GCGCAACCAG 
TCGATGCCGA GCATGGATGC ACCACTCGCG CCGGAAGGAA CCAACATGGC CCCCCCTCGC 
CCCCCTCCGG AAGGATTGCG CGAAGCTTGC ATCCAACTAC AGGATGGAGA CAACCCGCGA 
AAGAACCGTT CCTCCAAAGA CAATGGTTCT GCATCCAACT CGGGTGGCGA TCAAACCGAA 
CTTGGCGGAA CTGGGACTGG AAGCGGAGAT GGAAGTGGAA GCGGCGGTCG CACTGGTAAC 
AACACCTCGC CCCCGGGAGC TATTGCGCCG GACCAACGAC CAACCAGGCC TCGCGATTTG 
GACCCGGACC GCGTCCAGAT ACCATCCGAG AATATGGACT ACATCCGTCA TCTGGGCCTT 
GTATCTCCCG AATTCTTGCA GGGAAGCCGG ACCAGCTATC AAGATGTTGC TCCGGATGCG 
GAAGGCTGGG TGTATCTCAA TCTGCTTTGT AACCTGGCCC AGCTGCACAT GGTTAACGTA 
ACGCCGAGTT TTATTCGCCA AGCTGTCTCC GAAAAGAGCA CAAAGTTCCA GCTGTCTGCT 
GATGGTCGGA AAATCCGCTG GCGTGGCGGA ACCGATGGAA CCAAGTTCAG CAGCGACAGC 
AGCGAGGACA AGTCCCAGCA AAGCCCTATG ACCGAAGATA CCGAGGATGG CTCGGATAAG 
AATGGTCGGA GGAAGAAGCG GAAAACGCAG CAGGCGAGTT CGGAGATTGG GAGGTTCGGT 
CCCTCAAGGT CACCTTCGGA CACCTTTCAT TACAAACCGA TGTTTGTACA CCGTAATTCA 
TCCTCAATCG AGACCTCTTT GGAGGAGTCG ATGTCACAAG GCTCAGAGGA TGCTGTCGAT 
GAGAGCAACA TGGGTAACTC GAAGTGGGAT TTTAGCGGAT CGGGGACAAC GCAACAACGG 
AGGAAGCGTC GATACGATGG CGCCATCGTT TACTACACCG GCGCTCCATT TTGCACCGAT 
CTTTCAGGAG ACCCTGGCGA TATGTCGCCA ACAGCGCAAA TGACGGCCGG CCGCGAGGTC 
GAAGGATCCG GATCTGGTGA TGAAGTTGAA CATGTGCTGC AGCGGACGTT GTCTGGATCG 
TCTCTTCCTA TTAGGCCGCT CAGTGATGAC CGGGCCCGCG TGGCCGAGGT GTTGGATTTC 
GACCCCGGAA ACCCGCCTGA ACTCGTGGCC GACGACGGTT CCTCGCCGAA TGACGAGGAT 
TTCGTCTTCC CATGGTGCGA GGATCCTGCC AAGGTTCGGA TTCAGCCTAT TGCCAAAGAA 
GTCATGGAGC CCTCTGGTCT TGGTGGAGTA CTTCCTGACG ACCATTTTGT GATGCTCGTC 
ACCACTCGCC GCGTCGTCAG ACCTATCCTG CAGCGGCAGT TGTCACGCTC GACAACATCG 
GAAGATACGG CCGAATTCAT CGCCGAACGC TTGGCAGCGA TAAGAACGTC GTCGCCTCTG 
CCTCCTCGCA GTCACAGGCT GACTGTCGCG CCGTTGCAGG TTGAATACGT CTCTGGGCAA 
TTCCGTCGGC TGAATCCAGC CCCACTCCCT CCTCCGGCGA TTTTCTATCC CCCGTTCAGT 
ACCGATTCCA GCTGGGACGA TGGCGATGAT CTAGCTTCTG ACGATGAAGA AGTGGAGGAG 
GTGGAGGAAG ATTCTTACTC TGAGGGGCAG ATCTCGCGCC GAGCAAATCC TCATTTCTCG 
GACAACAACA CGTACATGCG GAAGGACGAT TTGGCGTTCG ATACCGAGAC TGATGTGCGG 
ATGGACAGCG ACGACAATCG CTTGTCGGAC AGCGGCCACA ACATGCGGGC GATGATGCCG 
CGGGCAGAAG CGGTGGATGG GGACGACAGC CCATTGGCAG CCGTCACCGG AAAAGAGGTG 
GACATGCTGC ACACTGGCAG CTCGGTTGCA ACCGCTGGTG GGGCAGAGAG TGGCTATAGT 
AGTTCGATGG AGGACGTCTC ATCCTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011395125.1
CDS	1056..4025
Translation	MADSGDKSQGMRPPPFDSRGHPLPRRASPDKSITLENHRLARDTSSRVTSSSALGVTESQPQLKSSPTRRNSSGESEPTNWFNQSNRNPAAAFHDESHIMEVDPPFYQKETDSSNEESRYPPGRNPVHPPGGVQLPGFRPVAAHSSAADDYRSVIDDLTVENKRLKEELKRYKQFGSDVMRKEKLFEIKVHGLPRRKKRELEATLRDFAASLGDSSESTSQRRKTGRHGTAVHSSGVSLSKHDSSSSSRSRPVDSAYNSMSTGRSSHAPHSSGPSLGRPSLTRAKSVGTQKVENYLRDTPDGLLPHHIVMTDKEKKKLVVRRLEQLFTGKISGRNMQRNQSMPSMDAPLAPEGTNMAPPRPPPEGLREACIQLQDGDNPRKNRSSKDNGSASNSGGDQTELGGTGTGSGDGSGSGGRTGNNTSPPGAIAPDQRPTRPRDLDPDRVQIPSENMDYIRHLGLVSPEFLQGSRTSYQDVAPDAEGWVYLNLLCNLAQLHMVNVTPSFIRQAVSEKSTKFQLSADGRKIRWRGGTDGTKFSSDSSEDKSQQSPMTEDTEDGSDKNGRRKKRKTQQASSEIGRFGPSRSPSDTFHYKPMFVHRNSSSIETSLEESMSQGSEDAVDESNMGNSKWDFSGSGTTQQRRKRRYDGAIVYYTGAPFCTDLSGDPGDMSPTAQMTAGREVEGSGSGDEVEHVLQRTLSGSSLPIRPLSDDRARVAEVLDFDPGNPPELVADDGSSPNDEDFVFPWCEDPAKVRIQPIAKEVMEPSGLGGVLPDDHFVMLVTTRRVVRPILQRQLSRSTTSEDTAEFIAERLAAIRTSSPLPPRSHRLTVAPLQVEYVSGQFRRLNPAPLPPPAIFYPPFSTDSSWDDGDDLASDDEEVEEVEEDSYSEGQISRRANPHFSDNNTYMRKDDLAFDTETDVRMDSDDNRLSDSGHNMRAMMPRAEAVDGDDSPLAAVTGKEVDMLHTGSSVATAGGAESGYSSSMEDVSSS

Target ORF information:

RefSeq Version	XM_011396823.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A period clock protein FRQ (frq), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396823.1

ORF Insert Sequence:

ATGGCGGATA GTGGGGATAA ATCCCAGGGC ATGCGCCCAC CACCCTTTGA CAGCCGTGGT 
CACCCTCTTC CCCGACGTGC TTCCCCAGAC AAATCAATAA CACTTGAAAA TCACCGCCTT 
GCGCGAGATA CTAGCAGCAG AGTTACTTCA TCTTCCGCAC TGGGAGTGAC CGAATCCCAA 
CCCCAACTCA AGAGCTCGCC CACCCGAAGG AATTCGTCTG GAGAGAGCGA GCCCACGAAT 
TGGTTTAATC AATCCAATCG TAACCCTGCC GCGGCCTTTC ATGACGAGAG CCATATCATG 
GAAGTCGATC CTCCTTTTTA TCAAAAGGAG ACTGACTCCT CCAACGAGGA ATCCAGGTAT 
CCGCCTGGAC GAAACCCTGT ACATCCGCCC GGTGGTGTCC AGTTACCCGG GTTCCGCCCG 
GTTGCTGCTC ACAGCAGCGC CGCCGATGAC TACCGCAGTG TCATTGACGA CTTGACTGTC 
GAAAACAAGC GGCTTAAGGA GGAACTGAAG AGGTATAAGC AGTTTGGTTC CGACGTGATG 
AGGAAAGAGA AGTTGTTTGA GATCAAGGTA CATGGCTTAC CTAGAAGAAA GAAACGTGAG 
TTGGAGGCAA CGCTCCGCGA CTTTGCTGCC AGTCTCGGGG ACTCGAGCGA ATCGACATCG 
CAGAGGAGAA AGACTGGCAG ACATGGAACG GCCGTTCACT CGTCTGGGGT TTCGTTGTCG 
AAACACGATT CGTCGTCATC GTCGCGCAGT CGTCCTGTCG ATTCTGCATA CAACTCTATG 
TCGACGGGCC GTAGCTCTCA TGCGCCCCAC TCTTCCGGTC CGTCGCTCGG ACGACCTTCG 
CTGACCAGGG CAAAATCCGT CGGGACCCAA AAGGTAGAAA ACTATCTGCG CGACACGCCC 
GATGGCCTGC TTCCTCACCA TATTGTCATG ACCGACAAGG AGAAGAAGAA GCTGGTTGTC 
CGACGCTTGG AGCAACTGTT CACCGGAAAG ATCAGTGGAC GAAACATGCA GCGCAACCAG 
TCGATGCCGA GCATGGATGC ACCACTCGCG CCGGAAGGAA CCAACATGGC CCCCCCTCGC 
CCCCCTCCGG AAGGATTGCG CGAAGCTTGC ATCCAACTAC AGGATGGAGA CAACCCGCGA 
AAGAACCGTT CCTCCAAAGA CAATGGTTCT GCATCCAACT CGGGTGGCGA TCAAACCGAA 
CTTGGCGGAA CTGGGACTGG AAGCGGAGAT GGAAGTGGAA GCGGCGGTCG CACTGGTAAC 
AACACCTCGC CCCCGGGAGC TATTGCGCCG GACCAACGAC CAACCAGGCC TCGCGATTTG 
GACCCGGACC GCGTCCAGAT ACCATCCGAG AATATGGACT ACATCCGTCA TCTGGGCCTT 
GTATCTCCCG AATTCTTGCA GGGAAGCCGG ACCAGCTATC AAGATGTTGC TCCGGATGCG 
GAAGGCTGGG TGTATCTCAA TCTGCTTTGT AACCTGGCCC AGCTGCACAT GGTTAACGTA 
ACGCCGAGTT TTATTCGCCA AGCTGTCTCC GAAAAGAGCA CAAAGTTCCA GCTGTCTGCT 
GATGGTCGGA AAATCCGCTG GCGTGGCGGA ACCGATGGAA CCAAGTTCAG CAGCGACAGC 
AGCGAGGACA AGTCCCAGCA AAGCCCTATG ACCGAAGATA CCGAGGATGG CTCGGATAAG 
AATGGTCGGA GGAAGAAGCG GAAAACGCAG CAGGCGAGTT CGGAGATTGG GAGGTTCGGT 
CCCTCAAGGT CACCTTCGGA CACCTTTCAT TACAAACCGA TGTTTGTACA CCGTAATTCA 
TCCTCAATCG AGACCTCTTT GGAGGAGTCG ATGTCACAAG GCTCAGAGGA TGCTGTCGAT 
GAGAGCAACA TGGGTAACTC GAAGTGGGAT TTTAGCGGAT CGGGGACAAC GCAACAACGG 
AGGAAGCGTC GATACGATGG CGCCATCGTT TACTACACCG GCGCTCCATT TTGCACCGAT 
CTTTCAGGAG ACCCTGGCGA TATGTCGCCA ACAGCGCAAA TGACGGCCGG CCGCGAGGTC 
GAAGGATCCG GATCTGGTGA TGAAGTTGAA CATGTGCTGC AGCGGACGTT GTCTGGATCG 
TCTCTTCCTA TTAGGCCGCT CAGTGATGAC CGGGCCCGCG TGGCCGAGGT GTTGGATTTC 
GACCCCGGAA ACCCGCCTGA ACTCGTGGCC GACGACGGTT CCTCGCCGAA TGACGAGGAT 
TTCGTCTTCC CATGGTGCGA GGATCCTGCC AAGGTTCGGA TTCAGCCTAT TGCCAAAGAA 
GTCATGGAGC CCTCTGGTCT TGGTGGAGTA CTTCCTGACG ACCATTTTGT GATGCTCGTC 
ACCACTCGCC GCGTCGTCAG ACCTATCCTG CAGCGGCAGT TGTCACGCTC GACAACATCG 
GAAGATACGG CCGAATTCAT CGCCGAACGC TTGGCAGCGA TAAGAACGTC GTCGCCTCTG 
CCTCCTCGCA GTCACAGGCT GACTGTCGCG CCGTTGCAGG TTGAATACGT CTCTGGGCAA 
TTCCGTCGGC TGAATCCAGC CCCACTCCCT CCTCCGGCGA TTTTCTATCC CCCGTTCAGT 
ACCGATTCCA GCTGGGACGA TGGCGATGAT CTAGCTTCTG ACGATGAAGA AGTGGAGGAG 
GTGGAGGAAG ATTCTTACTC TGAGGGGCAG ATCTCGCGCC GAGCAAATCC TCATTTCTCG 
GACAACAACA CGTACATGCG GAAGGACGAT TTGGCGTTCG ATACCGAGAC TGATGTGCGG 
ATGGACAGCG ACGACAATCG CTTGTCGGAC AGCGGCCACA ACATGCGGGC GATGATGCCG 
CGGGCAGAAG CGGTGGATGG GGACGACAGC CCATTGGCAG CCGTCACCGG AAAAGAGGTG 
GACATGCTGC ACACTGGCAG CTCGGTTGCA ACCGCTGGTG GGGCAGAGAG TGGCTATAGT 
AGTTCGATGG AGGACGTCTC ATCCTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frq ( XM_011396824.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011395126.1 Neurospora crassa OR74A period clock protein FRQ, variant 1 (frq), mRNA.

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CloneID	ONf11240
Clone ID Related Accession (Same CDS sequence)	XM_011396823.1 , XM_011396824.1
Accession Version	XM_011396824.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	period clock protein FRQ, variant 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507).

ATGGCGGATA GTGGGGATAA ATCCCAGGGC ATGCGCCCAC CACCCTTTGA CAGCCGTGGT 
CACCCTCTTC CCCGACGTGC TTCCCCAGAC AAATCAATAA CACTTGAAAA TCACCGCCTT 
GCGCGAGATA CTAGCAGCAG AGTTACTTCA TCTTCCGCAC TGGGAGTGAC CGAATCCCAA 
CCCCAACTCA AGAGCTCGCC CACCCGAAGG AATTCGTCTG GAGAGAGCGA GCCCACGAAT 
TGGTTTAATC AATCCAATCG TAACCCTGCC GCGGCCTTTC ATGACGAGAG CCATATCATG 
GAAGTCGATC CTCCTTTTTA TCAAAAGGAG ACTGACTCCT CCAACGAGGA ATCCAGGTAT 
CCGCCTGGAC GAAACCCTGT ACATCCGCCC GGTGGTGTCC AGTTACCCGG GTTCCGCCCG 
GTTGCTGCTC ACAGCAGCGC CGCCGATGAC TACCGCAGTG TCATTGACGA CTTGACTGTC 
GAAAACAAGC GGCTTAAGGA GGAACTGAAG AGGTATAAGC AGTTTGGTTC CGACGTGATG 
AGGAAAGAGA AGTTGTTTGA GATCAAGGTA CATGGCTTAC CTAGAAGAAA GAAACGTGAG 
TTGGAGGCAA CGCTCCGCGA CTTTGCTGCC AGTCTCGGGG ACTCGAGCGA ATCGACATCG 
CAGAGGAGAA AGACTGGCAG ACATGGAACG GCCGTTCACT CGTCTGGGGT TTCGTTGTCG 
AAACACGATT CGTCGTCATC GTCGCGCAGT CGTCCTGTCG ATTCTGCATA CAACTCTATG 
TCGACGGGCC GTAGCTCTCA TGCGCCCCAC TCTTCCGGTC CGTCGCTCGG ACGACCTTCG 
CTGACCAGGG CAAAATCCGT CGGGACCCAA AAGGTAGAAA ACTATCTGCG CGACACGCCC 
GATGGCCTGC TTCCTCACCA TATTGTCATG ACCGACAAGG AGAAGAAGAA GCTGGTTGTC 
CGACGCTTGG AGCAACTGTT CACCGGAAAG ATCAGTGGAC GAAACATGCA GCGCAACCAG 
TCGATGCCGA GCATGGATGC ACCACTCGCG CCGGAAGGAA CCAACATGGC CCCCCCTCGC 
CCCCCTCCGG AAGGATTGCG CGAAGCTTGC ATCCAACTAC AGGATGGAGA CAACCCGCGA 
AAGAACCGTT CCTCCAAAGA CAATGGTTCT GCATCCAACT CGGGTGGCGA TCAAACCGAA 
CTTGGCGGAA CTGGGACTGG AAGCGGAGAT GGAAGTGGAA GCGGCGGTCG CACTGGTAAC 
AACACCTCGC CCCCGGGAGC TATTGCGCCG GACCAACGAC CAACCAGGCC TCGCGATTTG 
GACCCGGACC GCGTCCAGAT ACCATCCGAG AATATGGACT ACATCCGTCA TCTGGGCCTT 
GTATCTCCCG AATTCTTGCA GGGAAGCCGG ACCAGCTATC AAGATGTTGC TCCGGATGCG 
GAAGGCTGGG TGTATCTCAA TCTGCTTTGT AACCTGGCCC AGCTGCACAT GGTTAACGTA 
ACGCCGAGTT TTATTCGCCA AGCTGTCTCC GAAAAGAGCA CAAAGTTCCA GCTGTCTGCT 
GATGGTCGGA AAATCCGCTG GCGTGGCGGA ACCGATGGAA CCAAGTTCAG CAGCGACAGC 
AGCGAGGACA AGTCCCAGCA AAGCCCTATG ACCGAAGATA CCGAGGATGG CTCGGATAAG 
AATGGTCGGA GGAAGAAGCG GAAAACGCAG CAGGCGAGTT CGGAGATTGG GAGGTTCGGT 
CCCTCAAGGT CACCTTCGGA CACCTTTCAT TACAAACCGA TGTTTGTACA CCGTAATTCA 
TCCTCAATCG AGACCTCTTT GGAGGAGTCG ATGTCACAAG GCTCAGAGGA TGCTGTCGAT 
GAGAGCAACA TGGGTAACTC GAAGTGGGAT TTTAGCGGAT CGGGGACAAC GCAACAACGG 
AGGAAGCGTC GATACGATGG CGCCATCGTT TACTACACCG GCGCTCCATT TTGCACCGAT 
CTTTCAGGAG ACCCTGGCGA TATGTCGCCA ACAGCGCAAA TGACGGCCGG CCGCGAGGTC 
GAAGGATCCG GATCTGGTGA TGAAGTTGAA CATGTGCTGC AGCGGACGTT GTCTGGATCG 
TCTCTTCCTA TTAGGCCGCT CAGTGATGAC CGGGCCCGCG TGGCCGAGGT GTTGGATTTC 
GACCCCGGAA ACCCGCCTGA ACTCGTGGCC GACGACGGTT CCTCGCCGAA TGACGAGGAT 
TTCGTCTTCC CATGGTGCGA GGATCCTGCC AAGGTTCGGA TTCAGCCTAT TGCCAAAGAA 
GTCATGGAGC CCTCTGGTCT TGGTGGAGTA CTTCCTGACG ACCATTTTGT GATGCTCGTC 
ACCACTCGCC GCGTCGTCAG ACCTATCCTG CAGCGGCAGT TGTCACGCTC GACAACATCG 
GAAGATACGG CCGAATTCAT CGCCGAACGC TTGGCAGCGA TAAGAACGTC GTCGCCTCTG 
CCTCCTCGCA GTCACAGGCT GACTGTCGCG CCGTTGCAGG TTGAATACGT CTCTGGGCAA 
TTCCGTCGGC TGAATCCAGC CCCACTCCCT CCTCCGGCGA TTTTCTATCC CCCGTTCAGT 
ACCGATTCCA GCTGGGACGA TGGCGATGAT CTAGCTTCTG ACGATGAAGA AGTGGAGGAG 
GTGGAGGAAG ATTCTTACTC TGAGGGGCAG ATCTCGCGCC GAGCAAATCC TCATTTCTCG 
GACAACAACA CGTACATGCG GAAGGACGAT TTGGCGTTCG ATACCGAGAC TGATGTGCGG 
ATGGACAGCG ACGACAATCG CTTGTCGGAC AGCGGCCACA ACATGCGGGC GATGATGCCG 
CGGGCAGAAG CGGTGGATGG GGACGACAGC CCATTGGCAG CCGTCACCGG AAAAGAGGTG 
GACATGCTGC ACACTGGCAG CTCGGTTGCA ACCGCTGGTG GGGCAGAGAG TGGCTATAGT 
AGTTCGATGG AGGACGTCTC ATCCTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011395126.1
CDS	899..3868
Translation	MADSGDKSQGMRPPPFDSRGHPLPRRASPDKSITLENHRLARDTSSRVTSSSALGVTESQPQLKSSPTRRNSSGESEPTNWFNQSNRNPAAAFHDESHIMEVDPPFYQKETDSSNEESRYPPGRNPVHPPGGVQLPGFRPVAAHSSAADDYRSVIDDLTVENKRLKEELKRYKQFGSDVMRKEKLFEIKVHGLPRRKKRELEATLRDFAASLGDSSESTSQRRKTGRHGTAVHSSGVSLSKHDSSSSSRSRPVDSAYNSMSTGRSSHAPHSSGPSLGRPSLTRAKSVGTQKVENYLRDTPDGLLPHHIVMTDKEKKKLVVRRLEQLFTGKISGRNMQRNQSMPSMDAPLAPEGTNMAPPRPPPEGLREACIQLQDGDNPRKNRSSKDNGSASNSGGDQTELGGTGTGSGDGSGSGGRTGNNTSPPGAIAPDQRPTRPRDLDPDRVQIPSENMDYIRHLGLVSPEFLQGSRTSYQDVAPDAEGWVYLNLLCNLAQLHMVNVTPSFIRQAVSEKSTKFQLSADGRKIRWRGGTDGTKFSSDSSEDKSQQSPMTEDTEDGSDKNGRRKKRKTQQASSEIGRFGPSRSPSDTFHYKPMFVHRNSSSIETSLEESMSQGSEDAVDESNMGNSKWDFSGSGTTQQRRKRRYDGAIVYYTGAPFCTDLSGDPGDMSPTAQMTAGREVEGSGSGDEVEHVLQRTLSGSSLPIRPLSDDRARVAEVLDFDPGNPPELVADDGSSPNDEDFVFPWCEDPAKVRIQPIAKEVMEPSGLGGVLPDDHFVMLVTTRRVVRPILQRQLSRSTTSEDTAEFIAERLAAIRTSSPLPPRSHRLTVAPLQVEYVSGQFRRLNPAPLPPPAIFYPPFSTDSSWDDGDDLASDDEEVEEVEEDSYSEGQISRRANPHFSDNNTYMRKDDLAFDTETDVRMDSDDNRLSDSGHNMRAMMPRAEAVDGDDSPLAAVTGKEVDMLHTGSSVATAGGAESGYSSSMEDVSSS

Target ORF information:

RefSeq Version	XM_011396824.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A period clock protein FRQ, variant 1 (frq), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396824.1

ORF Insert Sequence:

ATGGCGGATA GTGGGGATAA ATCCCAGGGC ATGCGCCCAC CACCCTTTGA CAGCCGTGGT 
CACCCTCTTC CCCGACGTGC TTCCCCAGAC AAATCAATAA CACTTGAAAA TCACCGCCTT 
GCGCGAGATA CTAGCAGCAG AGTTACTTCA TCTTCCGCAC TGGGAGTGAC CGAATCCCAA 
CCCCAACTCA AGAGCTCGCC CACCCGAAGG AATTCGTCTG GAGAGAGCGA GCCCACGAAT 
TGGTTTAATC AATCCAATCG TAACCCTGCC GCGGCCTTTC ATGACGAGAG CCATATCATG 
GAAGTCGATC CTCCTTTTTA TCAAAAGGAG ACTGACTCCT CCAACGAGGA ATCCAGGTAT 
CCGCCTGGAC GAAACCCTGT ACATCCGCCC GGTGGTGTCC AGTTACCCGG GTTCCGCCCG 
GTTGCTGCTC ACAGCAGCGC CGCCGATGAC TACCGCAGTG TCATTGACGA CTTGACTGTC 
GAAAACAAGC GGCTTAAGGA GGAACTGAAG AGGTATAAGC AGTTTGGTTC CGACGTGATG 
AGGAAAGAGA AGTTGTTTGA GATCAAGGTA CATGGCTTAC CTAGAAGAAA GAAACGTGAG 
TTGGAGGCAA CGCTCCGCGA CTTTGCTGCC AGTCTCGGGG ACTCGAGCGA ATCGACATCG 
CAGAGGAGAA AGACTGGCAG ACATGGAACG GCCGTTCACT CGTCTGGGGT TTCGTTGTCG 
AAACACGATT CGTCGTCATC GTCGCGCAGT CGTCCTGTCG ATTCTGCATA CAACTCTATG 
TCGACGGGCC GTAGCTCTCA TGCGCCCCAC TCTTCCGGTC CGTCGCTCGG ACGACCTTCG 
CTGACCAGGG CAAAATCCGT CGGGACCCAA AAGGTAGAAA ACTATCTGCG CGACACGCCC 
GATGGCCTGC TTCCTCACCA TATTGTCATG ACCGACAAGG AGAAGAAGAA GCTGGTTGTC 
CGACGCTTGG AGCAACTGTT CACCGGAAAG ATCAGTGGAC GAAACATGCA GCGCAACCAG 
TCGATGCCGA GCATGGATGC ACCACTCGCG CCGGAAGGAA CCAACATGGC CCCCCCTCGC 
CCCCCTCCGG AAGGATTGCG CGAAGCTTGC ATCCAACTAC AGGATGGAGA CAACCCGCGA 
AAGAACCGTT CCTCCAAAGA CAATGGTTCT GCATCCAACT CGGGTGGCGA TCAAACCGAA 
CTTGGCGGAA CTGGGACTGG AAGCGGAGAT GGAAGTGGAA GCGGCGGTCG CACTGGTAAC 
AACACCTCGC CCCCGGGAGC TATTGCGCCG GACCAACGAC CAACCAGGCC TCGCGATTTG 
GACCCGGACC GCGTCCAGAT ACCATCCGAG AATATGGACT ACATCCGTCA TCTGGGCCTT 
GTATCTCCCG AATTCTTGCA GGGAAGCCGG ACCAGCTATC AAGATGTTGC TCCGGATGCG 
GAAGGCTGGG TGTATCTCAA TCTGCTTTGT AACCTGGCCC AGCTGCACAT GGTTAACGTA 
ACGCCGAGTT TTATTCGCCA AGCTGTCTCC GAAAAGAGCA CAAAGTTCCA GCTGTCTGCT 
GATGGTCGGA AAATCCGCTG GCGTGGCGGA ACCGATGGAA CCAAGTTCAG CAGCGACAGC 
AGCGAGGACA AGTCCCAGCA AAGCCCTATG ACCGAAGATA CCGAGGATGG CTCGGATAAG 
AATGGTCGGA GGAAGAAGCG GAAAACGCAG CAGGCGAGTT CGGAGATTGG GAGGTTCGGT 
CCCTCAAGGT CACCTTCGGA CACCTTTCAT TACAAACCGA TGTTTGTACA CCGTAATTCA 
TCCTCAATCG AGACCTCTTT GGAGGAGTCG ATGTCACAAG GCTCAGAGGA TGCTGTCGAT 
GAGAGCAACA TGGGTAACTC GAAGTGGGAT TTTAGCGGAT CGGGGACAAC GCAACAACGG 
AGGAAGCGTC GATACGATGG CGCCATCGTT TACTACACCG GCGCTCCATT TTGCACCGAT 
CTTTCAGGAG ACCCTGGCGA TATGTCGCCA ACAGCGCAAA TGACGGCCGG CCGCGAGGTC 
GAAGGATCCG GATCTGGTGA TGAAGTTGAA CATGTGCTGC AGCGGACGTT GTCTGGATCG 
TCTCTTCCTA TTAGGCCGCT CAGTGATGAC CGGGCCCGCG TGGCCGAGGT GTTGGATTTC 
GACCCCGGAA ACCCGCCTGA ACTCGTGGCC GACGACGGTT CCTCGCCGAA TGACGAGGAT 
TTCGTCTTCC CATGGTGCGA GGATCCTGCC AAGGTTCGGA TTCAGCCTAT TGCCAAAGAA 
GTCATGGAGC CCTCTGGTCT TGGTGGAGTA CTTCCTGACG ACCATTTTGT GATGCTCGTC 
ACCACTCGCC GCGTCGTCAG ACCTATCCTG CAGCGGCAGT TGTCACGCTC GACAACATCG 
GAAGATACGG CCGAATTCAT CGCCGAACGC TTGGCAGCGA TAAGAACGTC GTCGCCTCTG 
CCTCCTCGCA GTCACAGGCT GACTGTCGCG CCGTTGCAGG TTGAATACGT CTCTGGGCAA 
TTCCGTCGGC TGAATCCAGC CCCACTCCCT CCTCCGGCGA TTTTCTATCC CCCGTTCAGT 
ACCGATTCCA GCTGGGACGA TGGCGATGAT CTAGCTTCTG ACGATGAAGA AGTGGAGGAG 
GTGGAGGAAG ATTCTTACTC TGAGGGGCAG ATCTCGCGCC GAGCAAATCC TCATTTCTCG 
GACAACAACA CGTACATGCG GAAGGACGAT TTGGCGTTCG ATACCGAGAC TGATGTGCGG 
ATGGACAGCG ACGACAATCG CTTGTCGGAC AGCGGCCACA ACATGCGGGC GATGATGCCG 
CGGGCAGAAG CGGTGGATGG GGACGACAGC CCATTGGCAG CCGTCACCGG AAAAGAGGTG 
GACATGCTGC ACACTGGCAG CTCGGTTGCA ACCGCTGGTG GGGCAGAGAG TGGCTATAGT 
AGTTCGATGG AGGACGTCTC ATCCTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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frq cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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