cdc24 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	cdc24
Entrez Gene ID	3875977
Full Name	cdc24-like
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_954737.3	XP_959830.3	rho guanyl nucleotide exchange factor

Pathways from BioSystems

Homologies

Neurospora crassa	NCU06067	XP_959830.2
Magnaporthe oryzae (rice blast fungus)	MGG_09697	XP_003717658.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study identifies CDC-24 as essential regulator for RAC and CDC-42 that have common and distinct functions during polarity establishment and maintenance of cell polarity in N. crassa.
Title: Functional characterization and cellular dynamics of the CDC-42 - RAC - CDC-24 module in Neurospora crassa.

The following cdc24 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cdc24 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf11205	XM_954737.3 Latest version!	Neurospora crassa OR74A rho guanyl nucleotide exchange factor (cdc24), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cdc24 ( XM_954737.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_959830.3 Neurospora crassa OR74A rho guanyl nucleotide exchange factor (cdc24), mRNA.

Price & Availability↑

CloneID	ONf11205
Clone ID Related Accession (Same CDS sequence)	XM_954737.3
Accession Version	XM_954737.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	rho guanyl nucleotide exchange factor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507). On Feb 23, 2015 this sequence version replaced XM_954737.2.

ATGGCCCATG CTGCTCTTCT ACGAACGAAC ACCGCCCCTG TATTTCAAGC GTCGACCGAC 
GGCGTCGCCA GATTGAACAA CATGAGCCTG TCCAACGCCG CGCGCGCGAG CCAGCTTTCG 
GGCTCGACGG CCTTCAATTC CTCTACTTCG CTCAGCTCGC TCACTTCGTC CGCCACCGCC 
GTTACCCCCG TGAACGGCCA GGTTGTCGCC ACCACAAACA TCATCAACCA GAGAGCCGAC 
GCCTCGCGAT CGCTGTACCA AATATGTTTG TCGCTCAAAC AACGCCTCGC CCAGGTTCCC 
GGCTTCGAGG GCTATCTAGA ACAACTGCAG CGCGAGCAGG AGAATGGCCA TGATGGCCCC 
GTCGAGGCCC TCTGGAAGCT CCTTCGCACC GGTCTGCCCC TGCTCACCAT CTACAATGCG 
CTCCAACCCG AGAAACCGCT ACACGTCGAT CAGAGGCCGA ACGAGACGGA AGCGCGACGG 
TCCAAACGCG CCATTATGGA ATTCGTCAAG GCCTGCTTGA GCGACCTCGA CCCTCCCGTT 
ACCGAATGCT TTATCGTCGC CGACCTTACA GGGGAGGATA CCACCGGCTT TGTCAAGGTG 
ACTTCTGTTA TCAACTATGT GCTCGATCTT GCCGAAAAAC GGGGCCTCTT GCTCCAAACC 
CTGCCATACC CCGAAGATGA TATCACGCAG CCGGGTTCTA AGATGACCCA TCGCGATTAC 
ATTGTTCGAG AACTGGTAGA CACGGAGCGC AAATACGTAC AGGATCTCGA GAACCTCCAC 
GACCTGAAGA GGACGCTGGA ACACAGGAGC GTTATCCCTG GCGAATTCAT CCACGACATA 
TTCCTTAATA TCAACGCCAT CTTGGATCAT CAACGAAAGT TCCTCATCAG GGTAGAAACA 
ACAAATTCGA TGCCGCAGGC TAGGCAGGAA TGGGGAAGCT TGTTCGTCAC AGCTGAAGAG 
AACTTTGGCA TTTACCAACC CTTTATCGCG AACCAAAGAA AGGCGGCCCA GGTTGCGACG 
CAAGTGTTTG ACAAGATCCA AGAGGCTGGC CATCCGGTCG CGTGTGATTT TAATACCCTT 
GATGGTTTCC TCCTCAAGCC TATGCAGAGA CTGGTCAAGT ACCCGCTTTT GCTCAAGGAT 
TTGCTGAAGA AGAGCGAGGA CGAGCACACC AAGGAGGATT TAGCTGCCGG CATCGCTGCT 
GCTGAACGGG TCTTGATGAA AGCCAATTCG GCCGTTGATA AGAACATCCT CGAAGAGGCC 
TTGCAGGACC TTATACACCG AGTAGACGAC TGGAAGAGCC ATAAGGTGGA CAACTTTGGA 
AGTCTGCTCC TACACGGAGT ATACACTGTT ATAACGGGCA AGAGTGAACA AGAGAAGGAC 
TATGAGATCT ATCTTTTTGA GAATATCTTG CTCTGTTGCA AGGAACTCAC TACGACTAAA 
GCCAAGGACA AGAAGGACAA GACTCGATCA TCCGTACCAA AGTTGCGGAA CAAGTTCGCC 
AAACTACAAC TTAAGGGCCG CATCTTCATG ACCAATGTCA CCGATGTAGT AGCCATTTCC 
AAGCCTGGTT CGTATACCGT ACAAATATGG TGGAAGGGCG ATCCGGGCGT GGAGAATTTC 
ATGATCAAAT TCCAGACCGA AGAGATGATG AAGAAGTGGG CCGCCGGTCT GGAAGAACAG 
CGAAAGCAGA ACACGCAAGC GGCCCCAGTT GCTCCTGATC AAGCTCCGCC AAACTTTGCC 
TGGCTCGCCT CGCAAGGCAA CGGACTGGAG AATCCCTACA AGGACGAAGA AGAGGACGAG 
GACATCTATA ATGCTGCTGC CCCGACCGTT GCCATGCCAG GCACAATGCC GCGGAATGGA 
TCGAGCACAA GTCTACGGCA GCGGTCCGCC ACCAGCGACA GTCTAGCAGG TATGGCTCGC 
GCACCACCTC CACGATTCCC TCTTCCACAG GCACCTGCGC CTCTCAATGT TGGCCTTCAG 
CCATCACCCG CTGTTCGAGC AGGCGAGTCT TACTTCTCTC CCGTAGCCGA GTCGCCCGCT 
TCGAGTCGGA CGAGCACAGC GAGCGGCATC TTCGCCGGTG CCGGATCCGG CTATCCTTTC 
CCCAAGACCG GTACCCCCCA AGGATGGCAG GAGGATAGCA ACCGCTACAC GGCCCCTGCC 
ATGCCAAGGG CGCCATCGCG AGACGGCCCA TCTCCAGGAA CCGGGAGGAA CCCACGAGGA 
CCCTCGATGC CCGTTATGCC CCGGGGGGAT AACAGCCAGT TTCAGCAGGC ACGCAGTCGG 
AGTTATAGTA CACCGGACAT TGCTGGCCAG GCCGCTGCGC GCATCAACCA GACAGGGGTA 
CCTGCGGTGC CAGGCATTCC CGCCCATCTA ACCCATGCCC GCCATGATTC CAACATTCCC 
AGGAGCAATA CCGGCAGTCC AGCAAACGAC CTTCCTCTAC GGACCCAGAC AGGTTCCCCT 
GGCAGAGAGC GGAAGAACTA TGGAGGCCAA TTGAATCAGT TCCCTCCACA GCCTATCCGC 
CAGGGAACAC CAGGCAGTGT CACCGGCTTC CCTCCGCCAT CTGGCCCGCC CCCACCGGGC 
ATGGCGCCTC TTGCCCCGAC TGAGGGAACG CGAAACATCA CCCCATCGCT CGGTACCGCA 
CCGATCATGT CCGGACAGAA CCCGCTCTCA CCCACAGATG ACGACATGCT CCCCACGCAA 
CTGAAGGTCA AGGTTCACTG CGATACCGGT AACTACATCA CATTGGTTGT GCTCTACGAC 
ATTACTTACG AGAAACTGAT TGACCGCATC GACAATAAAC TTTCGCGGTT TACCAGTAGC 
AGTATCGGCG GGGGTAATTT GAGGTTACGG TACCGGGATG AGGATGGCGA CTTCGTCATG 
ATCGAGAGCG ACGATGATAT TCAAATCGCC ATTTCGGATT GGCGTGAGGG GCTTCGCAAC 
CCAGATAGCG CCGGGGAAAT TGACCTGTAC TGCGAAGGGG AACTGTTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_959830.3
CDS	721..3714
Translation	MAHAALLRTNTAPVFQASTDGVARLNNMSLSNAARASQLSGSTAFNSSTSLSSLTSSATAVTPVNGQVVATTNIINQRADASRSLYQICLSLKQRLAQVPGFEGYLEQLQREQENGHDGPVEALWKLLRTGLPLLTIYNALQPEKPLHVDQRPNETEARRSKRAIMEFVKACLSDLDPPVTECFIVADLTGEDTTGFVKVTSVINYVLDLAEKRGLLLQTLPYPEDDITQPGSKMTHRDYIVRELVDTERKYVQDLENLHDLKRTLEHRSVIPGEFIHDIFLNINAILDHQRKFLIRVETTNSMPQARQEWGSLFVTAEENFGIYQPFIANQRKAAQVATQVFDKIQEAGHPVACDFNTLDGFLLKPMQRLVKYPLLLKDLLKKSEDEHTKEDLAAGIAAAERVLMKANSAVDKNILEEALQDLIHRVDDWKSHKVDNFGSLLLHGVYTVITGKSEQEKDYEIYLFENILLCCKELTTTKAKDKKDKTRSSVPKLRNKFAKLQLKGRIFMTNVTDVVAISKPGSYTVQIWWKGDPGVENFMIKFQTEEMMKKWAAGLEEQRKQNTQAAPVAPDQAPPNFAWLASQGNGLENPYKDEEEDEDIYNAAAPTVAMPGTMPRNGSSTSLRQRSATSDSLAGMARAPPPRFPLPQAPAPLNVGLQPSPAVRAGESYFSPVAESPASSRTSTASGIFAGAGSGYPFPKTGTPQGWQEDSNRYTAPAMPRAPSRDGPSPGTGRNPRGPSMPVMPRGDNSQFQQARSRSYSTPDIAGQAAARINQTGVPAVPGIPAHLTHARHDSNIPRSNTGSPANDLPLRTQTGSPGRERKNYGGQLNQFPPQPIRQGTPGSVTGFPPPSGPPPPGMAPLAPTEGTRNITPSLGTAPIMSGQNPLSPTDDDMLPTQLKVKVHCDTGNYITLVVLYDITYEKLIDRIDNKLSRFTSSSIGGGNLRLRYRDEDGDFVMIESDDDIQIAISDWREGLRNPDSAGEIDLYCEGELLE

Target ORF information:

RefSeq Version	XM_954737.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A rho guanyl nucleotide exchange factor (cdc24), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_954737.3

ORF Insert Sequence:

ATGGCCCATG CTGCTCTTCT ACGAACGAAC ACCGCCCCTG TATTTCAAGC GTCGACCGAC 
GGCGTCGCCA GATTGAACAA CATGAGCCTG TCCAACGCCG CGCGCGCGAG CCAGCTTTCG 
GGCTCGACGG CCTTCAATTC CTCTACTTCG CTCAGCTCGC TCACTTCGTC CGCCACCGCC 
GTTACCCCCG TGAACGGCCA GGTTGTCGCC ACCACAAACA TCATCAACCA GAGAGCCGAC 
GCCTCGCGAT CGCTGTACCA AATATGTTTG TCGCTCAAAC AACGCCTCGC CCAGGTTCCC 
GGCTTCGAGG GCTATCTAGA ACAACTGCAG CGCGAGCAGG AGAATGGCCA TGATGGCCCC 
GTCGAGGCCC TCTGGAAGCT CCTTCGCACC GGTCTGCCCC TGCTCACCAT CTACAATGCG 
CTCCAACCCG AGAAACCGCT ACACGTCGAT CAGAGGCCGA ACGAGACGGA AGCGCGACGG 
TCCAAACGCG CCATTATGGA ATTCGTCAAG GCCTGCTTGA GCGACCTCGA CCCTCCCGTT 
ACCGAATGCT TTATCGTCGC CGACCTTACA GGGGAGGATA CCACCGGCTT TGTCAAGGTG 
ACTTCTGTTA TCAACTATGT GCTCGATCTT GCCGAAAAAC GGGGCCTCTT GCTCCAAACC 
CTGCCATACC CCGAAGATGA TATCACGCAG CCGGGTTCTA AGATGACCCA TCGCGATTAC 
ATTGTTCGAG AACTGGTAGA CACGGAGCGC AAATACGTAC AGGATCTCGA GAACCTCCAC 
GACCTGAAGA GGACGCTGGA ACACAGGAGC GTTATCCCTG GCGAATTCAT CCACGACATA 
TTCCTTAATA TCAACGCCAT CTTGGATCAT CAACGAAAGT TCCTCATCAG GGTAGAAACA 
ACAAATTCGA TGCCGCAGGC TAGGCAGGAA TGGGGAAGCT TGTTCGTCAC AGCTGAAGAG 
AACTTTGGCA TTTACCAACC CTTTATCGCG AACCAAAGAA AGGCGGCCCA GGTTGCGACG 
CAAGTGTTTG ACAAGATCCA AGAGGCTGGC CATCCGGTCG CGTGTGATTT TAATACCCTT 
GATGGTTTCC TCCTCAAGCC TATGCAGAGA CTGGTCAAGT ACCCGCTTTT GCTCAAGGAT 
TTGCTGAAGA AGAGCGAGGA CGAGCACACC AAGGAGGATT TAGCTGCCGG CATCGCTGCT 
GCTGAACGGG TCTTGATGAA AGCCAATTCG GCCGTTGATA AGAACATCCT CGAAGAGGCC 
TTGCAGGACC TTATACACCG AGTAGACGAC TGGAAGAGCC ATAAGGTGGA CAACTTTGGA 
AGTCTGCTCC TACACGGAGT ATACACTGTT ATAACGGGCA AGAGTGAACA AGAGAAGGAC 
TATGAGATCT ATCTTTTTGA GAATATCTTG CTCTGTTGCA AGGAACTCAC TACGACTAAA 
GCCAAGGACA AGAAGGACAA GACTCGATCA TCCGTACCAA AGTTGCGGAA CAAGTTCGCC 
AAACTACAAC TTAAGGGCCG CATCTTCATG ACCAATGTCA CCGATGTAGT AGCCATTTCC 
AAGCCTGGTT CGTATACCGT ACAAATATGG TGGAAGGGCG ATCCGGGCGT GGAGAATTTC 
ATGATCAAAT TCCAGACCGA AGAGATGATG AAGAAGTGGG CCGCCGGTCT GGAAGAACAG 
CGAAAGCAGA ACACGCAAGC GGCCCCAGTT GCTCCTGATC AAGCTCCGCC AAACTTTGCC 
TGGCTCGCCT CGCAAGGCAA CGGACTGGAG AATCCCTACA AGGACGAAGA AGAGGACGAG 
GACATCTATA ATGCTGCTGC CCCGACCGTT GCCATGCCAG GCACAATGCC GCGGAATGGA 
TCGAGCACAA GTCTACGGCA GCGGTCCGCC ACCAGCGACA GTCTAGCAGG TATGGCTCGC 
GCACCACCTC CACGATTCCC TCTTCCACAG GCACCTGCGC CTCTCAATGT TGGCCTTCAG 
CCATCACCCG CTGTTCGAGC AGGCGAGTCT TACTTCTCTC CCGTAGCCGA GTCGCCCGCT 
TCGAGTCGGA CGAGCACAGC GAGCGGCATC TTCGCCGGTG CCGGATCCGG CTATCCTTTC 
CCCAAGACCG GTACCCCCCA AGGATGGCAG GAGGATAGCA ACCGCTACAC GGCCCCTGCC 
ATGCCAAGGG CGCCATCGCG AGACGGCCCA TCTCCAGGAA CCGGGAGGAA CCCACGAGGA 
CCCTCGATGC CCGTTATGCC CCGGGGGGAT AACAGCCAGT TTCAGCAGGC ACGCAGTCGG 
AGTTATAGTA CACCGGACAT TGCTGGCCAG GCCGCTGCGC GCATCAACCA GACAGGGGTA 
CCTGCGGTGC CAGGCATTCC CGCCCATCTA ACCCATGCCC GCCATGATTC CAACATTCCC 
AGGAGCAATA CCGGCAGTCC AGCAAACGAC CTTCCTCTAC GGACCCAGAC AGGTTCCCCT 
GGCAGAGAGC GGAAGAACTA TGGAGGCCAA TTGAATCAGT TCCCTCCACA GCCTATCCGC 
CAGGGAACAC CAGGCAGTGT CACCGGCTTC CCTCCGCCAT CTGGCCCGCC CCCACCGGGC 
ATGGCGCCTC TTGCCCCGAC TGAGGGAACG CGAAACATCA CCCCATCGCT CGGTACCGCA 
CCGATCATGT CCGGACAGAA CCCGCTCTCA CCCACAGATG ACGACATGCT CCCCACGCAA 
CTGAAGGTCA AGGTTCACTG CGATACCGGT AACTACATCA CATTGGTTGT GCTCTACGAC 
ATTACTTACG AGAAACTGAT TGACCGCATC GACAATAAAC TTTCGCGGTT TACCAGTAGC 
AGTATCGGCG GGGGTAATTT GAGGTTACGG TACCGGGATG AGGATGGCGA CTTCGTCATG 
ATCGAGAGCG ACGATGATAT TCAAATCGCC ATTTCGGATT GGCGTGAGGG GCTTCGCAAC 
CCAGATAGCG CCGGGGAAAT TGACCTGTAC TGCGAAGGGG AACTGTTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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cdc24 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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