pks-3 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	pks-3
Entrez Gene ID	3875270
Full Name	polyketide synthase-3
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_954029.2	XP_959122.2	polyketide synthase 3

Pathways from BioSystems

Homologies

Neurospora crassa	NCU04865	XP_959122.1
Magnaporthe oryzae (rice blast fungus)	MGG_13767	XP_003710791.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pks-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pks-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf10934	XM_954029.2 Latest version!	Neurospora crassa OR74A polyketide synthase 3 (pks-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pks-3 ( XM_954029.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_959122.2 Neurospora crassa OR74A polyketide synthase 3 (pks-3), mRNA.

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CloneID	ONf10934
Clone ID Related Accession (Same CDS sequence)	XM_954029.2
Accession Version	XM_954029.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	polyketide synthase 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). On Feb 23, 2015 this sequence version replaced XM_954029.1.

ATGGATTCCA ACATGCCTAT TGCCATTATT GGCATGAGCT GCCGATTCGC TGGCGATGTC 
GACAGTCCGT CCAAGCTCTG GGATCTCTTG GCTCAAGGAA AGAGTGCGTG GTCGGAGATT 
CCCAAGGATC GGTTCAATAT CGATGGATTC CACCACCCCA ACTTTGAGAA GCTTAATGGT 
ACAAATGTAA TAGGCGGCCA CTTTATGAAG GAAGATGTTG GCCTGTTTGA TGCGCACTTT 
TTTAACCTTT CTGCCGAGAC TGCTGCTGCT TTGGACCCCC AATTCCGTCT GCAGCTCGAG 
TCAACATACG AGGCTCTTGA GAGCGCGGGT ATTACTCTTC AGGATGTGGC TGGCTCCAAC 
ACGTCGGTCT ACGCCGGCTC CTTCTTCCGG GACTACCACG AGAGCTTGAT CCGTGACCCC 
GACACACTTC CCCGCTTCCT CCTCATGGGC ACCGGCGCCG CCATGGCTTC CAACCGTCTC 
TCGCACTTTT TTGACCTCCG CGGGCCCAGC ATGTCGGTCG ACACGGGCTG CTCCACCACC 
CTCACAGCGC TTCACCAGGC CTGCCAGAGC TTGCGTTCGG GCGAATCCAC CATGTCGATT 
GTGGGCGGCG CCAACATCAT GTTCAACCCC GACATGTTCC TCGCCATGTC CTCCATGACG 
CTCATCTCCA AGGACGGCCG GTCCTGGGCC TTTGACAGCC GCGCCAACGG CTACGGCCGC 
GGCGAGGGAT CGGCCACGGT GGTTCTCAAG CCCCTTGACG CCGCTCTGCG CGACGGCGAC 
CCCATCCGCG CCGTTATCCG CGACAGCGGC ATCAACCAGG ACGGCAAGAC CGAGACCATC 
ACCACGCCCA GCGGCGAGGC ACAGGAGGCC TTGATCCGCG CCTGCTACGA GCGCGCAGGT 
CTCGATCCGG GACAGACGAC GTACTTTGAG GCACACGGAA CGGGTACTCC GACGGGCGAT 
CCAATCGAGG TGAAGGCCAT CGCCAGGGTG TTCAAGGACT CGCGCAAGGG CAACGGCGAG 
GACGCGCTGC TGCGTATCGG GTCCGTCAAG ACCAACATTG GCCACACCGA GACGGCCTCG 
GGCGTGGCGG CCATCATCAA GGTGGCCTTG GCGCTGGAAA GGGGCCAGAT CCCGCCGAGC 
ATCAACTTTG AGACGCCGAA TGCCAAGTTG AGTTTGGACG AGTGGAAGCT CAAAGTGCCC 
ACCGAGTTGG AGGAGTGGGT GGGCAAGGAC GGCATCAGGC GGGCCAGCAT TAACAATTTC 
GGCTATGGCG GGTCGAATGC CCACGTTATT ATGGAGGATT ACAGCAGCTA CCTCGCCACG 
ACTCAGCGGC CCAAGGCGCT ACCTCTGACG AATGGCACTG CCCCTGGCCA CCACCACCAC 
CACCACCACC AACACACCGA TAGCGGAATT GTCATTGACG ACGACTTCCA CGGCAACGGC 
AACAATGGCG ACCACCACCA CTCCAAGGTC TTCCTCCTCT CCGCCAAAGA CGAAAAAGCC 
ACGGAGCGCA TGATCGCCAA CCTCAAGACG TACCTCCACC AGCAGAAGGC CAACACCCAC 
TCCAAGATCG CCGAGAATGC CCTCCTCTCC AACCTCGCCC ACACCCTCTG CGACCGCCGC 
ACCCTCTTCC CCTGGACCGC CACCTTCTCG GGCGCCTCTC TCGACTCCCT CATCCGCACC 
CTCGACTCCG GCCGCGTCAA GCCCGCCAAG GCCTCGGCCC AGCCACCGCG CATCGGCTTC 
GTCTTCACCG GCCAAGGCGC CCAGTGGTGG GCCATGGGCA AGGAGCTCAT CGACGCCTAC 
CCCATCTTCA AGGCCGCCTT GCTCGACTGC GACGTCCAGC TCAAGAAACT CGGGGCCACC 
TGGAACATGT GCGAAGAGCT GTCGCGCGAT GCCGAGACCA GCAAGGTCAA CCAGCTCGAC 
TACAGCACGC CCGTGTGCGT CGCCGTGCAG ATTGCCTTGG TCGAGCTGCT GAAGGCGTGG 
GGCATCAAGC CGACGGCTGT CACGTCCCAC TCGTCGGGCG AGATTGCCGC TGCTTATGCC 
GCTGGTGCGC TGGACTTGGC GAGCGCCATG GCCATTGCCT TTGCCAGAGG AGGGTTGGCT 
AGCGAGGGTA ACCGCCAGTT TGCGAGAAAG GGAGGCATGA TGGCTGTCGG GCTGGGACGG 
GAGGAAGCCG AGAAGTACCT TCCTAGAGTT ACCCAGGGTC AGGTGGTCGT TGCCTGCGAG 
AACTCGCCGA CGAGCAGCAC CCTGTCGGGT GATGTCGAGG GTTTGGTTGA GCTGGAGCAG 
ATCATGAAGG AGGAGAACAT CTTTGCCAGA CGGCTGAAAG TTGATGCTGC CTGGCATTCG 
CATCACATGG AGGCTGTCGC TGATGCGTAC TATGCGTCCA TGGATAAGAA GGTCAAGCCG 
GCCAAGAGCA AGCTGGACAT GATCTTCTCT TCTCCTTGCA CCGGCAAGCG GATGGACAAG 
GTTAACGAGA TTGGCAGCCC GGGACACTGG GTGCGCTCGT TGACGGGCTG TGTCCGGTTT 
GTGGATGCGT TCCGCAGCAT GGTCTTTGCT GAGGAGGGTG CCACAGAGCC GACGGTTGAT 
ATGGTGATCG AGGTCGGTCC CCACGCGGCG CTGTCGGGTC CCATCCAGGA TATCTTGGGT 
ATGCCCCAGT TCAAGGGGGT TACTATCCCG TATGGCAGCT GCTTGATTCG CAAGAAGAGC 
GCGGTTGATA CCATGCAGGA TTTGGTCGGC GACCTGGTTC GCAAGGGGTA TCCTGTCAAC 
CTCAAGGCTG TCAACTTCCC CTTTGGCATG GAGGGTGTCA AGGTGTTGAC TGACTTGCCT 
GCCTACCCCT GGAATCACTC GACCAAGCAC TGGATTGAGC CGCGCTTCAA CAGGGCCCTC 
AGACAGCGTT CCGAGGCTCC TCATGACTTG CTTGGCTCGC TGGTGCTGGG CTGTGATCCT 
AGTGCTCCTA CTTGGCGCCA CATTGTCCGT CTCGGTGACC TTCCTTGGGC CAAGGACCAT 
TGTGTCCAGG GCAACATGAT CTATCCCGCT GCTGGATACA TTGCCATGGC TGTTGAGGGC 
ATGCAGCGCT TCGCTTCCCG TCAAGCGGGC GACAAGAAGA TTGCTGGCTA CCAGCTTCGT 
GATGTTGACA TCCTCAACGC TCTGGTTGTT CCCGAGACGA GCGAGGGTAT CGAGATGCAG 
CTTTCTCTTC GCCCCGGCAG TGAGCGGGAT CTATCTACCA AGGGATGGAC CGAGTTCACT 
GTTCAGTCCG TCAACTTGGA CAACAAGTGG ACTGATCACT GCAAGGGCTT GATCTGGGTT 
CAGTTCGGCG CTGCTCCCAA GCACGCCGTC AACCAGCCTA CCCAGGACTC CCACTACCGC 
ATCCGTATCA ACCCCAATGA CATCTGGGCT GGCATGCGCT CGGGTGGTAT CAACCATGGT 
CCCATCTTCC GCAACATGAA GAGCATTCGT GCCCGCAGCA AGCAGTCCGT CACCACCTTC 
ACCGTCGCCG ACACCAAGTC CGTCATGCCC AAGCAGCACG AGCATGCCCA TGTCATCCAC 
CCCACCACTT TGGATTCGGT CTTCCAAGCC GCTTACACTG CTGCCCCCGG CGCTGGTGGC 
AAGAACCAGA CCCCCAAGGT TCCTCGCTCC ATCAGCAAGC TCTGGATCTC CCACGACATC 
AGCAAGCAAG CCGGCCACGA CTTCAAGGCC TACGCCAACC TCGACCACGC CGACGACCAA 
TCCACCAAGA CTGCTCTCCG CGTCGTCGAT GATGCTGCCG ATGCTGCCGC GCCTGTCATT 
TCCATCGACG GCTTTGTCTG CCAATCCATC GGCAACGCGC CCACCGCCGC CGACGAGCCC 
TGGGAAGCCG ACAAGTTCAC CACCACCCAC TGGGCGCCCG ACGTGACCTT CCTCAAGGAC 
GCCTTCCTCA AGAAACAGCT CGGCAGCCAA ATCACGCCCG AGGAGGCCGA GATCCTCATG 
GACCTCCGCA AGGCGTGCAT GTACTACATC TACGACGCCC TCCGCGACCT CACCCCCGAG 
GACATCAAGA AGCTCGAGTG GTACCACAAG AAGTTCTACA TCTGGATGCG TCTGCAGGCC 
GACTTGGCGC GCGCCAACGA GCTGGGCCCC GATAGCAGCA AATGGGCCAA CGCCACGCTG 
AACGAGAAGG CCATCCTGCT GGAGAAGGTC AAGATGAGCA GCACCAACGG CGAGATGGTG 
TACCGTCTTG GTCCGCAGAT CGTGCCCATC CTGAGGGGAG AGATCACGGC CCTAGAGGTG 
ATGCTCGAGC AGAACTTGCT GTCCCGCTAC TACCTCGAAG GCCTCAAATG GGGTCGCGCC 
AACGCCAAGC TGGGCGAAAT GGTCCGCCAC TACGCGCACA AGAACCCCCA CGCGAAGATG 
ATTGAGATTG GTGGCGGTAC CGGCGGTGCC ACGGCTCATG TCCTCAACGC CATCGGCACG 
GCCGACGATG GACTGGGCCC CCGCGCTGCT AGCTACGACT TCACCGATGT ATCGTCGGGC 
TTCTTCGAGG CGGCCAAGGA AAAGTTCCAG CCGTGGAAGG ACCTGATGCG CTTCAAGAAG 
CTCAACATTG AGCAGGATCC TGTGTCGCAA GGGTTCGAGG AGGGAACGTA CGATGTGGTG 
ATTGCTTGCC AGGTTCTGCA CGCGACAAAG TCAATGGATA ACACGATGCA GAACGTGCGC 
AAGCTCCTCA AGCCCGGCGG TAAGCTGTTC ATCATGGAGA CGACCCAGGA CCAGATGGAT 
GTCCAGTTTG TGTTTGGTTT CCTTTCCGGG TGGTGGTTGA GCGAGGAGGA GGAGCGCAAG 
TTCAGCCCGT CGCTTTCCGT GCCCATGTGG GATCGCGTCT TGCATCGCAC TGGCTTCCGC 
GGTGTTGAGG CGGAGATCAG GGATGTCGAG CATGATGAGC TTTATGCGTT TAGCGTCATG 
AGCTCGACGG CTGCGGCTAG TGCTCCTGTA TTTGACTTTG ACATCACCTT TGTCACGGCG 
AGCAACCAGA TTCCCGAGGT GTGGTTGGAT AAGCTGAGGG TGTCAATTGG GTTGTTGACC 
TGCTCCGTGC CAACGGTGAA GAGCCTCGAG GAGGTCACGG TTGATGGAAA CGATGTCTGC 
GTCTTCCTCG ATGATCCCAA GAACCCCGTT CTGGCGGACC CGAGCAAGGC GCAGTTCAGT 
GGCGTCAGAG ACATGTGCAC TCGCGCCAAG GGCCTGCTTT GGTTGACGCA GGGCGGCGCC 
GATGAGTGCG ATCAGCCTTT GGCTTCGCTC GCTGCTGGAT TCCTCCGCTC TTTGAGGCAG 
GAGTACTCCG GCAAGCGTCT GGCCACACTC GATCTCGACC CGATCCAGGA CATGTGGTCC 
GAGACGTCCA TCACCACCAT CACCGAGGTG TTCCGCAAGT TGTTCGACTA CTCGATCAAC 
GAAGCCGGAA GCGACTACGA GTTTGCCGAG CGCGAGGGAG CCGTCAAGAT CCCACGCTAC 
ATCAAGGACG TCACGCGCAA CTCGGCCGTC TTCAAGCAAC AGCAAGCCAC CACCCAACCC 
GAAGCCGAGA TGCAGCCTTT CATCCAGCCT GACCGCCCCT TGCGTCTAGC CATTGGCACC 
GCCGGCTTGC TTGACACACT CAAATTCGTC GACGACCCGA CGGCGTCTGA CCCTCTTCCC 
GAGGACTTTG TCGAAGTCGA GCCGCGCGCG TTCGGCGTCA ACTTCCGCGA CGTCATGGTT 
GCCATGGGCC AACTCAAGTC CAACACCATG GGCTACGACT GCGCCGGCGT CATCACGCGC 
GTTGGTCCCG CCGCTGCTGC TGAAGGTTAC CAAGTTGGTG ACCGCGTCTC TGTTTTGCTC 
AGAGGTCACT ACGCCAGCAG GACGCGCATC CACTGGACCA GCGCCGTCAA GATCCCCGAT 
ACGATGACGT TTGAGACGGC CGCTAGCTTG CCGACGCAAT ACGTCGCTGC TTACGTTTCC 
TTGTATGACA CTGCTCGTCT GCAAAAGGGC GAGTCGGTCC TCATCCATGC TGCCACGGGC 
GGTGTCGGTC AAGCGGCGGT GATGATGGCG CAGCGCGTGG GCGCCGAGGT GTTTGTGACT 
GTTGGCACGG ACGAGAAGCG CGAGTTTGTC ATGAAGCACT ACGGGATTGC GGCCGACCAT 
ATCTTTTCCT CGCGCGATAC TTCCTTCGCT GCAGGCGTCA TGGCCATGAC CAAGGGCCGG 
GGCGTGGATG TGGTGCTTAA CTCGCTGGCT GGCACTCTTT TGCAGGAATC TTTCAACTGC 
TTGGCTCCCT TTGGCCGCTT CGTGGAGATT GGCAAACGCG ATCTGGAGCA GAACAGCAGT 
CTGGCGATGG AGGCGTTCAC GCGGGCGGTG TCATACACAA GCATTGACGT GATCACACTG 
GGAGAGTACA AGAAGATGGA GACGAACCGG ATCATGAAGG ACATTATCCA CTTGGTTGGG 
GCTGGAGAGT TGCGGGTGGT TGAGCCGATC ACAGTTTATC CCGTCGGAGA GATTGAGAAG 
GCATTCAGGT TGATGCAGGC TGGCAAGCAC ATGGGTAAGA TCGTGTTGAC TGCCTCAGAG 
GAGACCTTGG TGCCGGTCCT CCCGCGTGCC ACCTCCAACA CCGTCTCTCT CCGCTCCGAC 
GCGTCCTACC TCGTCGTCGG CGGCTTCGGC GGTCTCGGTC GCTCCATCTG CTCCTGGCTC 
GCCGAGCACG GAGCCAAGAA TCTTGTCATT GTCTCCCGCA ACGCCAAAGC CGACAAGCTC 
GCCCAGCTAC AGACCGAACT GAACCACGTC TCCAAGGGCG TCAAGGTCAC CGCCATCAGC 
TGCGACATCT CCAACATGGC TGTCCTTACC AAAGCCCTCG ACTGCGTCGA GCGCGGCGTG 
CCTCCCATCC GCGGCATCAT CCACGGCGGC ATGGAACTGC GCGACTCCGT CCTCGAACAC 
ATGAAACTAG AAGATCACAA GGCCGCTCTG GCGCCCAAAC TCAACGGCTC CTGGAACTTG 
CACCAGTATT TCTCCTCTAC TGAAAAAGAG AGTCTAGACT TCTACATCAT GCTCTCCTCT 
CTCGTCGGCG TCGTCGGCTT CGCTTCCCAA TCCAACTACT CCGCAGGCGG CACCTTCCAA 
GACAGCCTCG CCACCCACCG CGTCGCTCAC GGTCTTCCCG GTGTCTCGCT CGACTTGCCC 
GTCGTCAAAT CCGTCGGTTA CCTCGCCGAC CACCCCGACT CGGAAAAGAC CATTGACTCG 
CTCAAGCGCC ACGGCTTCAC TGCCTTGGCA GAGGACGAAG TCCTAGCAGC CATTGGCTCT 
GCGATTCAGA CCCCCTTCGC AGGCCCCCTC ACCCTCGGTC TCAACTGCGG ACCGTCCGGG 
CAACCATCCC TTGATGCTCC TCTAGCGCGC GACGCCCGTT TCGCACAGCT TAAACACCGC 
GCTGTCAAGT CTTCTTCCAC CTCCCAATCT TCAGTTTCAG CCACCACGGG GGATCTATCC 
AGTCTGCTGG CTTCGTCTCA AAGTATCGAA GAAGCAGTCA CCCACACGCT GGAAGCCATC 
AGCAAGAAAT TGCAAGATAT CTTTATGATT GCGGACGCGA GCGAGATCAA CCCGGATTGT 
TCGCCCGCAG AGTTTGGGGT GGATAGTCTG GTGGCGGTGG AGTTGAGGAA CATGCTGAGT 
ATGAAGGCGG GGAGCGAGAT GAGTATCTTT GAGATTATGC AGTGTGGGAG TTTGAAGGGG 
TTGGCGGGGG TGGTGGTGGG GAAGAGTGGG TTTGTTGATG ATGGGTTGAA GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_959122.2
CDS	313..8049
Translation	MDSNMPIAIIGMSCRFAGDVDSPSKLWDLLAQGKSAWSEIPKDRFNIDGFHHPNFEKLNGTNVIGGHFMKEDVGLFDAHFFNLSAETAAALDPQFRLQLESTYEALESAGITLQDVAGSNTSVYAGSFFRDYHESLIRDPDTLPRFLLMGTGAAMASNRLSHFFDLRGPSMSVDTGCSTTLTALHQACQSLRSGESTMSIVGGANIMFNPDMFLAMSSMTLISKDGRSWAFDSRANGYGRGEGSATVVLKPLDAALRDGDPIRAVIRDSGINQDGKTETITTPSGEAQEALIRACYERAGLDPGQTTYFEAHGTGTPTGDPIEVKAIARVFKDSRKGNGEDALLRIGSVKTNIGHTETASGVAAIIKVALALERGQIPPSINFETPNAKLSLDEWKLKVPTELEEWVGKDGIRRASINNFGYGGSNAHVIMEDYSSYLATTQRPKALPLTNGTAPGHHHHHHHQHTDSGIVIDDDFHGNGNNGDHHHSKVFLLSAKDEKATERMIANLKTYLHQQKANTHSKIAENALLSNLAHTLCDRRTLFPWTATFSGASLDSLIRTLDSGRVKPAKASAQPPRIGFVFTGQGAQWWAMGKELIDAYPIFKAALLDCDVQLKKLGATWNMCEELSRDAETSKVNQLDYSTPVCVAVQIALVELLKAWGIKPTAVTSHSSGEIAAAYAAGALDLASAMAIAFARGGLASEGNRQFARKGGMMAVGLGREEAEKYLPRVTQGQVVVACENSPTSSTLSGDVEGLVELEQIMKEENIFARRLKVDAAWHSHHMEAVADAYYASMDKKVKPAKSKLDMIFSSPCTGKRMDKVNEIGSPGHWVRSLTGCVRFVDAFRSMVFAEEGATEPTVDMVIEVGPHAALSGPIQDILGMPQFKGVTIPYGSCLIRKKSAVDTMQDLVGDLVRKGYPVNLKAVNFPFGMEGVKVLTDLPAYPWNHSTKHWIEPRFNRALRQRSEAPHDLLGSLVLGCDPSAPTWRHIVRLGDLPWAKDHCVQGNMIYPAAGYIAMAVEGMQRFASRQAGDKKIAGYQLRDVDILNALVVPETSEGIEMQLSLRPGSERDLSTKGWTEFTVQSVNLDNKWTDHCKGLIWVQFGAAPKHAVNQPTQDSHYRIRINPNDIWAGMRSGGINHGPIFRNMKSIRARSKQSVTTFTVADTKSVMPKQHEHAHVIHPTTLDSVFQAAYTAAPGAGGKNQTPKVPRSISKLWISHDISKQAGHDFKAYANLDHADDQSTKTALRVVDDAADAAAPVISIDGFVCQSIGNAPTAADEPWEADKFTTTHWAPDVTFLKDAFLKKQLGSQITPEEAEILMDLRKACMYYIYDALRDLTPEDIKKLEWYHKKFYIWMRLQADLARANELGPDSSKWANATLNEKAILLEKVKMSSTNGEMVYRLGPQIVPILRGEITALEVMLEQNLLSRYYLEGLKWGRANAKLGEMVRHYAHKNPHAKMIEIGGGTGGATAHVLNAIGTADDGLGPRAASYDFTDVSSGFFEAAKEKFQPWKDLMRFKKLNIEQDPVSQGFEEGTYDVVIACQVLHATKSMDNTMQNVRKLLKPGGKLFIMETTQDQMDVQFVFGFLSGWWLSEEEERKFSPSLSVPMWDRVLHRTGFRGVEAEIRDVEHDELYAFSVMSSTAAASAPVFDFDITFVTASNQIPEVWLDKLRVSIGLLTCSVPTVKSLEEVTVDGNDVCVFLDDPKNPVLADPSKAQFSGVRDMCTRAKGLLWLTQGGADECDQPLASLAAGFLRSLRQEYSGKRLATLDLDPIQDMWSETSITTITEVFRKLFDYSINEAGSDYEFAEREGAVKIPRYIKDVTRNSAVFKQQQATTQPEAEMQPFIQPDRPLRLAIGTAGLLDTLKFVDDPTASDPLPEDFVEVEPRAFGVNFRDVMVAMGQLKSNTMGYDCAGVITRVGPAAAAEGYQVGDRVSVLLRGHYASRTRIHWTSAVKIPDTMTFETAASLPTQYVAAYVSLYDTARLQKGESVLIHAATGGVGQAAVMMAQRVGAEVFVTVGTDEKREFVMKHYGIAADHIFSSRDTSFAAGVMAMTKGRGVDVVLNSLAGTLLQESFNCLAPFGRFVEIGKRDLEQNSSLAMEAFTRAVSYTSIDVITLGEYKKMETNRIMKDIIHLVGAGELRVVEPITVYPVGEIEKAFRLMQAGKHMGKIVLTASEETLVPVLPRATSNTVSLRSDASYLVVGGFGGLGRSICSWLAEHGAKNLVIVSRNAKADKLAQLQTELNHVSKGVKVTAISCDISNMAVLTKALDCVERGVPPIRGIIHGGMELRDSVLEHMKLEDHKAALAPKLNGSWNLHQYFSSTEKESLDFYIMLSSLVGVVGFASQSNYSAGGTFQDSLATHRVAHGLPGVSLDLPVVKSVGYLADHPDSEKTIDSLKRHGFTALAEDEVLAAIGSAIQTPFAGPLTLGLNCGPSGQPSLDAPLARDARFAQLKHRAVKSSSTSQSSVSATTGDLSSLLASSQSIEEAVTHTLEAISKKLQDIFMIADASEINPDCSPAEFGVDSLVAVELRNMLSMKAGSEMSIFEIMQCGSLKGLAGVVVGKSGFVDDGLKE

Target ORF information:

RefSeq Version	XM_954029.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A polyketide synthase 3 (pks-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_954029.2

ORF Insert Sequence:

ATGGATTCCA ACATGCCTAT TGCCATTATT GGCATGAGCT GCCGATTCGC TGGCGATGTC 
GACAGTCCGT CCAAGCTCTG GGATCTCTTG GCTCAAGGAA AGAGTGCGTG GTCGGAGATT 
CCCAAGGATC GGTTCAATAT CGATGGATTC CACCACCCCA ACTTTGAGAA GCTTAATGGT 
ACAAATGTAA TAGGCGGCCA CTTTATGAAG GAAGATGTTG GCCTGTTTGA TGCGCACTTT 
TTTAACCTTT CTGCCGAGAC TGCTGCTGCT TTGGACCCCC AATTCCGTCT GCAGCTCGAG 
TCAACATACG AGGCTCTTGA GAGCGCGGGT ATTACTCTTC AGGATGTGGC TGGCTCCAAC 
ACGTCGGTCT ACGCCGGCTC CTTCTTCCGG GACTACCACG AGAGCTTGAT CCGTGACCCC 
GACACACTTC CCCGCTTCCT CCTCATGGGC ACCGGCGCCG CCATGGCTTC CAACCGTCTC 
TCGCACTTTT TTGACCTCCG CGGGCCCAGC ATGTCGGTCG ACACGGGCTG CTCCACCACC 
CTCACAGCGC TTCACCAGGC CTGCCAGAGC TTGCGTTCGG GCGAATCCAC CATGTCGATT 
GTGGGCGGCG CCAACATCAT GTTCAACCCC GACATGTTCC TCGCCATGTC CTCCATGACG 
CTCATCTCCA AGGACGGCCG GTCCTGGGCC TTTGACAGCC GCGCCAACGG CTACGGCCGC 
GGCGAGGGAT CGGCCACGGT GGTTCTCAAG CCCCTTGACG CCGCTCTGCG CGACGGCGAC 
CCCATCCGCG CCGTTATCCG CGACAGCGGC ATCAACCAGG ACGGCAAGAC CGAGACCATC 
ACCACGCCCA GCGGCGAGGC ACAGGAGGCC TTGATCCGCG CCTGCTACGA GCGCGCAGGT 
CTCGATCCGG GACAGACGAC GTACTTTGAG GCACACGGAA CGGGTACTCC GACGGGCGAT 
CCAATCGAGG TGAAGGCCAT CGCCAGGGTG TTCAAGGACT CGCGCAAGGG CAACGGCGAG 
GACGCGCTGC TGCGTATCGG GTCCGTCAAG ACCAACATTG GCCACACCGA GACGGCCTCG 
GGCGTGGCGG CCATCATCAA GGTGGCCTTG GCGCTGGAAA GGGGCCAGAT CCCGCCGAGC 
ATCAACTTTG AGACGCCGAA TGCCAAGTTG AGTTTGGACG AGTGGAAGCT CAAAGTGCCC 
ACCGAGTTGG AGGAGTGGGT GGGCAAGGAC GGCATCAGGC GGGCCAGCAT TAACAATTTC 
GGCTATGGCG GGTCGAATGC CCACGTTATT ATGGAGGATT ACAGCAGCTA CCTCGCCACG 
ACTCAGCGGC CCAAGGCGCT ACCTCTGACG AATGGCACTG CCCCTGGCCA CCACCACCAC 
CACCACCACC AACACACCGA TAGCGGAATT GTCATTGACG ACGACTTCCA CGGCAACGGC 
AACAATGGCG ACCACCACCA CTCCAAGGTC TTCCTCCTCT CCGCCAAAGA CGAAAAAGCC 
ACGGAGCGCA TGATCGCCAA CCTCAAGACG TACCTCCACC AGCAGAAGGC CAACACCCAC 
TCCAAGATCG CCGAGAATGC CCTCCTCTCC AACCTCGCCC ACACCCTCTG CGACCGCCGC 
ACCCTCTTCC CCTGGACCGC CACCTTCTCG GGCGCCTCTC TCGACTCCCT CATCCGCACC 
CTCGACTCCG GCCGCGTCAA GCCCGCCAAG GCCTCGGCCC AGCCACCGCG CATCGGCTTC 
GTCTTCACCG GCCAAGGCGC CCAGTGGTGG GCCATGGGCA AGGAGCTCAT CGACGCCTAC 
CCCATCTTCA AGGCCGCCTT GCTCGACTGC GACGTCCAGC TCAAGAAACT CGGGGCCACC 
TGGAACATGT GCGAAGAGCT GTCGCGCGAT GCCGAGACCA GCAAGGTCAA CCAGCTCGAC 
TACAGCACGC CCGTGTGCGT CGCCGTGCAG ATTGCCTTGG TCGAGCTGCT GAAGGCGTGG 
GGCATCAAGC CGACGGCTGT CACGTCCCAC TCGTCGGGCG AGATTGCCGC TGCTTATGCC 
GCTGGTGCGC TGGACTTGGC GAGCGCCATG GCCATTGCCT TTGCCAGAGG AGGGTTGGCT 
AGCGAGGGTA ACCGCCAGTT TGCGAGAAAG GGAGGCATGA TGGCTGTCGG GCTGGGACGG 
GAGGAAGCCG AGAAGTACCT TCCTAGAGTT ACCCAGGGTC AGGTGGTCGT TGCCTGCGAG 
AACTCGCCGA CGAGCAGCAC CCTGTCGGGT GATGTCGAGG GTTTGGTTGA GCTGGAGCAG 
ATCATGAAGG AGGAGAACAT CTTTGCCAGA CGGCTGAAAG TTGATGCTGC CTGGCATTCG 
CATCACATGG AGGCTGTCGC TGATGCGTAC TATGCGTCCA TGGATAAGAA GGTCAAGCCG 
GCCAAGAGCA AGCTGGACAT GATCTTCTCT TCTCCTTGCA CCGGCAAGCG GATGGACAAG 
GTTAACGAGA TTGGCAGCCC GGGACACTGG GTGCGCTCGT TGACGGGCTG TGTCCGGTTT 
GTGGATGCGT TCCGCAGCAT GGTCTTTGCT GAGGAGGGTG CCACAGAGCC GACGGTTGAT 
ATGGTGATCG AGGTCGGTCC CCACGCGGCG CTGTCGGGTC CCATCCAGGA TATCTTGGGT 
ATGCCCCAGT TCAAGGGGGT TACTATCCCG TATGGCAGCT GCTTGATTCG CAAGAAGAGC 
GCGGTTGATA CCATGCAGGA TTTGGTCGGC GACCTGGTTC GCAAGGGGTA TCCTGTCAAC 
CTCAAGGCTG TCAACTTCCC CTTTGGCATG GAGGGTGTCA AGGTGTTGAC TGACTTGCCT 
GCCTACCCCT GGAATCACTC GACCAAGCAC TGGATTGAGC CGCGCTTCAA CAGGGCCCTC 
AGACAGCGTT CCGAGGCTCC TCATGACTTG CTTGGCTCGC TGGTGCTGGG CTGTGATCCT 
AGTGCTCCTA CTTGGCGCCA CATTGTCCGT CTCGGTGACC TTCCTTGGGC CAAGGACCAT 
TGTGTCCAGG GCAACATGAT CTATCCCGCT GCTGGATACA TTGCCATGGC TGTTGAGGGC 
ATGCAGCGCT TCGCTTCCCG TCAAGCGGGC GACAAGAAGA TTGCTGGCTA CCAGCTTCGT 
GATGTTGACA TCCTCAACGC TCTGGTTGTT CCCGAGACGA GCGAGGGTAT CGAGATGCAG 
CTTTCTCTTC GCCCCGGCAG TGAGCGGGAT CTATCTACCA AGGGATGGAC CGAGTTCACT 
GTTCAGTCCG TCAACTTGGA CAACAAGTGG ACTGATCACT GCAAGGGCTT GATCTGGGTT 
CAGTTCGGCG CTGCTCCCAA GCACGCCGTC AACCAGCCTA CCCAGGACTC CCACTACCGC 
ATCCGTATCA ACCCCAATGA CATCTGGGCT GGCATGCGCT CGGGTGGTAT CAACCATGGT 
CCCATCTTCC GCAACATGAA GAGCATTCGT GCCCGCAGCA AGCAGTCCGT CACCACCTTC 
ACCGTCGCCG ACACCAAGTC CGTCATGCCC AAGCAGCACG AGCATGCCCA TGTCATCCAC 
CCCACCACTT TGGATTCGGT CTTCCAAGCC GCTTACACTG CTGCCCCCGG CGCTGGTGGC 
AAGAACCAGA CCCCCAAGGT TCCTCGCTCC ATCAGCAAGC TCTGGATCTC CCACGACATC 
AGCAAGCAAG CCGGCCACGA CTTCAAGGCC TACGCCAACC TCGACCACGC CGACGACCAA 
TCCACCAAGA CTGCTCTCCG CGTCGTCGAT GATGCTGCCG ATGCTGCCGC GCCTGTCATT 
TCCATCGACG GCTTTGTCTG CCAATCCATC GGCAACGCGC CCACCGCCGC CGACGAGCCC 
TGGGAAGCCG ACAAGTTCAC CACCACCCAC TGGGCGCCCG ACGTGACCTT CCTCAAGGAC 
GCCTTCCTCA AGAAACAGCT CGGCAGCCAA ATCACGCCCG AGGAGGCCGA GATCCTCATG 
GACCTCCGCA AGGCGTGCAT GTACTACATC TACGACGCCC TCCGCGACCT CACCCCCGAG 
GACATCAAGA AGCTCGAGTG GTACCACAAG AAGTTCTACA TCTGGATGCG TCTGCAGGCC 
GACTTGGCGC GCGCCAACGA GCTGGGCCCC GATAGCAGCA AATGGGCCAA CGCCACGCTG 
AACGAGAAGG CCATCCTGCT GGAGAAGGTC AAGATGAGCA GCACCAACGG CGAGATGGTG 
TACCGTCTTG GTCCGCAGAT CGTGCCCATC CTGAGGGGAG AGATCACGGC CCTAGAGGTG 
ATGCTCGAGC AGAACTTGCT GTCCCGCTAC TACCTCGAAG GCCTCAAATG GGGTCGCGCC 
AACGCCAAGC TGGGCGAAAT GGTCCGCCAC TACGCGCACA AGAACCCCCA CGCGAAGATG 
ATTGAGATTG GTGGCGGTAC CGGCGGTGCC ACGGCTCATG TCCTCAACGC CATCGGCACG 
GCCGACGATG GACTGGGCCC CCGCGCTGCT AGCTACGACT TCACCGATGT ATCGTCGGGC 
TTCTTCGAGG CGGCCAAGGA AAAGTTCCAG CCGTGGAAGG ACCTGATGCG CTTCAAGAAG 
CTCAACATTG AGCAGGATCC TGTGTCGCAA GGGTTCGAGG AGGGAACGTA CGATGTGGTG 
ATTGCTTGCC AGGTTCTGCA CGCGACAAAG TCAATGGATA ACACGATGCA GAACGTGCGC 
AAGCTCCTCA AGCCCGGCGG TAAGCTGTTC ATCATGGAGA CGACCCAGGA CCAGATGGAT 
GTCCAGTTTG TGTTTGGTTT CCTTTCCGGG TGGTGGTTGA GCGAGGAGGA GGAGCGCAAG 
TTCAGCCCGT CGCTTTCCGT GCCCATGTGG GATCGCGTCT TGCATCGCAC TGGCTTCCGC 
GGTGTTGAGG CGGAGATCAG GGATGTCGAG CATGATGAGC TTTATGCGTT TAGCGTCATG 
AGCTCGACGG CTGCGGCTAG TGCTCCTGTA TTTGACTTTG ACATCACCTT TGTCACGGCG 
AGCAACCAGA TTCCCGAGGT GTGGTTGGAT AAGCTGAGGG TGTCAATTGG GTTGTTGACC 
TGCTCCGTGC CAACGGTGAA GAGCCTCGAG GAGGTCACGG TTGATGGAAA CGATGTCTGC 
GTCTTCCTCG ATGATCCCAA GAACCCCGTT CTGGCGGACC CGAGCAAGGC GCAGTTCAGT 
GGCGTCAGAG ACATGTGCAC TCGCGCCAAG GGCCTGCTTT GGTTGACGCA GGGCGGCGCC 
GATGAGTGCG ATCAGCCTTT GGCTTCGCTC GCTGCTGGAT TCCTCCGCTC TTTGAGGCAG 
GAGTACTCCG GCAAGCGTCT GGCCACACTC GATCTCGACC CGATCCAGGA CATGTGGTCC 
GAGACGTCCA TCACCACCAT CACCGAGGTG TTCCGCAAGT TGTTCGACTA CTCGATCAAC 
GAAGCCGGAA GCGACTACGA GTTTGCCGAG CGCGAGGGAG CCGTCAAGAT CCCACGCTAC 
ATCAAGGACG TCACGCGCAA CTCGGCCGTC TTCAAGCAAC AGCAAGCCAC CACCCAACCC 
GAAGCCGAGA TGCAGCCTTT CATCCAGCCT GACCGCCCCT TGCGTCTAGC CATTGGCACC 
GCCGGCTTGC TTGACACACT CAAATTCGTC GACGACCCGA CGGCGTCTGA CCCTCTTCCC 
GAGGACTTTG TCGAAGTCGA GCCGCGCGCG TTCGGCGTCA ACTTCCGCGA CGTCATGGTT 
GCCATGGGCC AACTCAAGTC CAACACCATG GGCTACGACT GCGCCGGCGT CATCACGCGC 
GTTGGTCCCG CCGCTGCTGC TGAAGGTTAC CAAGTTGGTG ACCGCGTCTC TGTTTTGCTC 
AGAGGTCACT ACGCCAGCAG GACGCGCATC CACTGGACCA GCGCCGTCAA GATCCCCGAT 
ACGATGACGT TTGAGACGGC CGCTAGCTTG CCGACGCAAT ACGTCGCTGC TTACGTTTCC 
TTGTATGACA CTGCTCGTCT GCAAAAGGGC GAGTCGGTCC TCATCCATGC TGCCACGGGC 
GGTGTCGGTC AAGCGGCGGT GATGATGGCG CAGCGCGTGG GCGCCGAGGT GTTTGTGACT 
GTTGGCACGG ACGAGAAGCG CGAGTTTGTC ATGAAGCACT ACGGGATTGC GGCCGACCAT 
ATCTTTTCCT CGCGCGATAC TTCCTTCGCT GCAGGCGTCA TGGCCATGAC CAAGGGCCGG 
GGCGTGGATG TGGTGCTTAA CTCGCTGGCT GGCACTCTTT TGCAGGAATC TTTCAACTGC 
TTGGCTCCCT TTGGCCGCTT CGTGGAGATT GGCAAACGCG ATCTGGAGCA GAACAGCAGT 
CTGGCGATGG AGGCGTTCAC GCGGGCGGTG TCATACACAA GCATTGACGT GATCACACTG 
GGAGAGTACA AGAAGATGGA GACGAACCGG ATCATGAAGG ACATTATCCA CTTGGTTGGG 
GCTGGAGAGT TGCGGGTGGT TGAGCCGATC ACAGTTTATC CCGTCGGAGA GATTGAGAAG 
GCATTCAGGT TGATGCAGGC TGGCAAGCAC ATGGGTAAGA TCGTGTTGAC TGCCTCAGAG 
GAGACCTTGG TGCCGGTCCT CCCGCGTGCC ACCTCCAACA CCGTCTCTCT CCGCTCCGAC 
GCGTCCTACC TCGTCGTCGG CGGCTTCGGC GGTCTCGGTC GCTCCATCTG CTCCTGGCTC 
GCCGAGCACG GAGCCAAGAA TCTTGTCATT GTCTCCCGCA ACGCCAAAGC CGACAAGCTC 
GCCCAGCTAC AGACCGAACT GAACCACGTC TCCAAGGGCG TCAAGGTCAC CGCCATCAGC 
TGCGACATCT CCAACATGGC TGTCCTTACC AAAGCCCTCG ACTGCGTCGA GCGCGGCGTG 
CCTCCCATCC GCGGCATCAT CCACGGCGGC ATGGAACTGC GCGACTCCGT CCTCGAACAC 
ATGAAACTAG AAGATCACAA GGCCGCTCTG GCGCCCAAAC TCAACGGCTC CTGGAACTTG 
CACCAGTATT TCTCCTCTAC TGAAAAAGAG AGTCTAGACT TCTACATCAT GCTCTCCTCT 
CTCGTCGGCG TCGTCGGCTT CGCTTCCCAA TCCAACTACT CCGCAGGCGG CACCTTCCAA 
GACAGCCTCG CCACCCACCG CGTCGCTCAC GGTCTTCCCG GTGTCTCGCT CGACTTGCCC 
GTCGTCAAAT CCGTCGGTTA CCTCGCCGAC CACCCCGACT CGGAAAAGAC CATTGACTCG 
CTCAAGCGCC ACGGCTTCAC TGCCTTGGCA GAGGACGAAG TCCTAGCAGC CATTGGCTCT 
GCGATTCAGA CCCCCTTCGC AGGCCCCCTC ACCCTCGGTC TCAACTGCGG ACCGTCCGGG 
CAACCATCCC TTGATGCTCC TCTAGCGCGC GACGCCCGTT TCGCACAGCT TAAACACCGC 
GCTGTCAAGT CTTCTTCCAC CTCCCAATCT TCAGTTTCAG CCACCACGGG GGATCTATCC 
AGTCTGCTGG CTTCGTCTCA AAGTATCGAA GAAGCAGTCA CCCACACGCT GGAAGCCATC 
AGCAAGAAAT TGCAAGATAT CTTTATGATT GCGGACGCGA GCGAGATCAA CCCGGATTGT 
TCGCCCGCAG AGTTTGGGGT GGATAGTCTG GTGGCGGTGG AGTTGAGGAA CATGCTGAGT 
ATGAAGGCGG GGAGCGAGAT GAGTATCTTT GAGATTATGC AGTGTGGGAG TTTGAAGGGG 
TTGGCGGGGG TGGTGGTGGG GAAGAGTGGG TTTGTTGATG ATGGGTTGAA GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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pks-3 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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