crf1-1 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	crf1-1
Entrez Gene ID	3874449
Full Name	chromatin remodelling factor 1-1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_953209.2	XP_958302.1	helicase swr-1

Pathways from BioSystems

Homologies

Neurospora crassa	NCU09993	XP_958302.1
Magnaporthe oryzae (rice blast fungus)	MGG_02183	XP_003708950.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following crf1-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crf1-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf00302	XM_953209.2 Latest version!	Neurospora crassa OR74A helicase swr-1 (crf1-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crf1-1 ( XM_953209.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_958302.1 Neurospora crassa OR74A helicase swr-1 (crf1-1), mRNA.

Price & Availability↑

CloneID	ONf00302
Clone ID Related Accession (Same CDS sequence)	XM_953209.2
Accession Version	XM_953209.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	helicase swr-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507). On Feb 23, 2015 this sequence version replaced XM_953209.1.

ATGACTACTA TGATGACCGA CTCTGGCACC GCGTCTGATT CTGGTGCGGG ACTTGTTGAT 
TCAACACGAA ACGACACCAC CACCACCACC ACCACCACCA CCACTCCCGG TGACAACGAC 
GCCGACGACG ACAACACCAA CGGCACTACT ACTGGTCATC ACGACAACAA CGAGACAGAC 
AACAACAGCA AAAGTTACAG CAGCACCCAC CATGTCCCCG CCATCGATAA TACCTCGACG 
ACCAACGCCA ACGACAACGA AGATGCTGCT GGCTTCCTCG ACCGCGACCA GTCGCCCTTG 
TCGTCCATCT CGTCCCCGCT GAGCGAACCC GACAACTTTG AGTTTGATAC CGAACCGATT 
CCATCCATCC TATCACCAAA GTCTACGACA AGCGATAATC ATGCCCGCGA CGGAGAGACA 
CCCGAGCTCG ACGAAGATGG ACAACCACCG GCCAAGCGAC GCCGAGTGCG CGAGACAACA 
CCTCACAATC ACTCGCGCAA ACCGAAACCA GAGTCACCAC CATGGAAGAA GTTTGAGGCC 
GAGGGTCCGA CTACCATCAT CACCGAAGAC GGTCGTCGCA AGTCTGGCCG CGTCAATCCC 
GTCTTGCTAG GCATGAAACC CAGCGACAAA AAGGTTACGC GGAAAGCTAT CCAAACAAGT 
CCTGTCAGCA ACAAAAGTAG TGCCAGTACG TCCAGGAAGC CAGCGCCAGC AAGCAGCAGC 
AACAGCAAAC ATGCTCCCGC AAAGATGCCG CCCCCGCCGC CGCCGCCGAA AGCTCCCGCG 
CGCAAGTCTG CCACGACTCA TGAAACCAGA CCCTCCGCGT CCCGATCCCG TCGACGATCT 
CCATCTCCTC GACGTCCTGC TACACCACCG AAACCCGCCG CTGTCGGTAC GCGTCGGTCA 
ACGCGCCAAG CCCTAGCCCA TTCTCGAGCA TCCTACGATG ATGGCCAGCT CTCTCCCGGT 
GCCTCGCGCG CCACTCCCCG TATCAAGCTC AAAGTTCGAC CCCCGTTGAC CGTAATACCT 
CTTGTTCATC CAAACCAAGC CAATGTTCGT CCAAAGCTTG GGCCGACCTT TGAGGAATAC 
TTTGCGCGCG CTCATGAAAT ACCGGTTGAG GAGGGTGGGC AGCACATTCC CGATGAAGAG 
CCCAAATACA CAGACGAGAT GGCCCTCCAA GACGCCAAGG TCATTCTACG GGTTGAAAAG 
GAGGTTGAGC CAGGGGGCTT GCTCGCCCCT GATAGGTGTT CGGCTTTCGA GCCTGAAGCC 
GAGGAGGAAC CGCCTCGACA GTGGGCTCAC CTTGACCATC TCACAAAGGC CATGACCAAC 
TTCCGCAAGT TGATGTATAG AGAGCAGCAG TTGCATATGC AGGCAGCCAA GCGCATCGCC 
ATTGCATGCG AAGCCGAGTG GAGGCGCCGA AACCCACAGC CCAAGACTGC CGAAGAAATC 
GAGCTGGAGG AGATGGAAGC GTCCAAGGCC AAATGGCGCC AGGTTATCAG GGCCTTTGCT 
GGAACATGGG AGAATGTCAG AGTCGAGGTC AATAGGCGCC GCTTGGTGGA ATGGGAGGCG 
GAGGAACAGC GCCGTGTCAA GGCCGCGCTG AATCAAGCAG TCAATCTTTC GGAACAAAAG 
CTACAGCAAC GACAAGCGCA AGTGGACGGG GACGAAATCA CAGACGAGGA TGAGGACGAG 
GACGACGAAG ACCTGGCAAG TGGCATGCCG TCAGTAATGG GCGACGAGAA GGAAAGTGAC 
GAGCACTCCG ACCAAGGATC CGATGAGATG TCCGACGAAA ATGACGAAGA TGAAGACGAG 
GATAACATGT CCTCCTCCGA GGACGACGAT AAGAAATCGA CCGCCTCGGA CGAGGGCCTG 
ACTCAGGAAG AATTGCGTGC AAAATACGCC AACCTACCCA CATTGGGCAC GACCGATGAA 
ACTGAAGAAA CAACAAAAGA CGTGGAAATG GCTGATGCTC CCGCTGCTAT GGATGGTAGC 
GTTGCGAATG ATGACGATAC AAGCGATGAG TCGGTAGACA TGGACGACGA CCTTGGATCT 
AGCGAGGAGT CAGACGACGA CGAGGAGGAG GAAGAAGACG ATGAGGAAGA GGAAGAGTCC 
GATGATGAAC CTGCTGGCTT GCTAGGGCTA TTCTTTGGAA AGTCAGAGTT GAAGAAGTTG 
AAGGAAGAGG CTGTAACTGA GGAGCCGTCG GTTGAACCCG CTGGGGATGT GGAGATGACT 
GATGCTTCGG CGGCCCTTCG TCCCGAGCTC CAAGTTAATG GTCATGCGCA TGAGGCCCCG 
ATAACTAATG GTACTCACAC TAATGAGCAG TTGGCATCAT CTCAAACTGA GGGTGCAGAC 
GATGAAGTGT CGTTACTGGT TATACCCAAC GACGAAATAG CAGCAGAAAA TACTCAAACC 
GCAGCAGAGC CGGGCAGCGG AGTGGTGGAG AAGGACTTCC TGACCAATGA TAGTTCCTTG 
AAGTATCCTA ATGAGATTGT ACAGTCCGAA AACCAGACAC TTCCGGCCAA GGAGTCGGTT 
GAAGCTGGAG GCGACCTGCC AATGGTTGAT GCCCCATCCA CAGACGATTT ACAAAGGGAA 
ACAGCCGCGG CGCCGTCGTT GGAGGCACCA CCCAACACCC GACATGATAG TCAGGAAACG 
GTGGCGGCTA CTGACATGCA GAGCCAGTCA CAAACACAGT CACCAAAAAC AACTGATACC 
AAGCCCACCG ACGTCGACAC TCCCCATTCA GAGCTGGCCG TCAGCGTGCA AAAGCCAGAT 
AGCCGCCAAT CCTCACCTCA GCCAACAACA CCAACAGTCA AGACCGAAAT CCCGTTTCTC 
TTACGTGGAA CACTGCGTGA GTATCAACAT CATGGTCTGG ACTGGCTTGC TGGGCTGTAT 
GCCAACAACA CCAATGGTAT CCTGGCCGAC GAGATGGGTT TGGGAAAGAC CATCCAGACG 
ATTGCTCTGC TGGCGCACCT CGCCTGTCAT CATGAAGTTT GGGGCCCCCA TCTGGTTATT 
GTGCCTACGA GTGTTATGCT CAACTGGGAA ATGGAGTTCA AGAAGTGGTG TCCTGGATTC 
AAGATCCTTA CGTACTACGG CAACCAGGAA GAGCGAAAGC GAAAGCGCCA GGGATGGAAC 
AACGACGATG TGTGGAACGT CTGCATCACC TCCTACCAAA TGGTCCTTCA GGACCAACAG 
GTGTTTAGGC GAAGACGGTG GCATTACATG ATCCTCGACG AGGCTCACAA CATCAAGAAT 
TTCAAGTCGC AGCGCTGGCA AACCCTGCTC GGCTTCAACA CGCAAGCTCG GTTACTTTTG 
ACAGGTACTC CGTTACAGAA CAACCTGACC GAGCTGTGGT CGCTTCTTTA CTTCTTGGCG 
CCACCCGAGA ACGGCGAAGG CGGGTTCGTT GACCTCACCG AATTCCATAA CTGGTTCGCT 
AGGCCCGAAT CTCAGATCTT GGAAAGCGGG CGTGAACAGC TGGACGACGA GGCGCGTGCT 
ATCATTGCCA AGCTGCACAA GGTCCTTCGA CCCTACCTTC TCCGTCGACT CAAGGCCGAT 
GTCGAGAAAC AGATGCCGGC AAAGTATGAA CACGTCGAGT TCTGCCGCCT CTCCAAACGT 
CAGCGAGAGT TGTATGATGG GTTCCTCTCC CGTGCCGATA CACGCGAGAC GCTGCAGTCG 
GGTAACTACA TGTCCATCAT TAACTGTTTG ATGCAGCTCC GAAAGGTCTG CAACCATCCC 
GATCTCTTTG TGGACCGGCC CATCATGACC TCGTTCCGCA TGTCGCGGTC AGTACCGGCC 
GACTACGAGT GGAAAGAAAA GTTCATTCGG AACAGGCTGT TGGCTACGAA GCCCATGACC 
ACTGTCAATT TGAGCTTCCT CAACATGATT CCGACCGAGT ACGAAGATCT GTCCAAGACA 
CACACCGACC GCATCGCCCA GCTCAGCTCC CATCGTATCT TGCTCGACCT GAGGGAGGCC 
CAGAAGGTGC GCGCCAACAA CGCCTACACC GCGCTGGACC CTTCCTCTGT CAAGTCCAAC 
CTTGTGTATC TTGAAAGTGC CGCCAGGTGG GGTCGGTTCG AAGAGCTGCA GCACTGTGTA 
TACATCAATG CCCTCCGTCG CCAACAACGA CCCATTTATG GAAAGCGTCT AACCGAGTTC 
CTCACCCTTG ATACCCATCT CCGACCATAC AAACCCCGAC CTAGAGTGCC GGCTAAGATC 
ATGTCCTGGT TCGAAGAGGA TTCCTTCCTG CTACACAACG CCATCCCAAC GCTCCAGCAA 
CGCGCCGAAT CCATGGAAAT GACCATCACC AAGTTCGCCT GCGTCACTCC CGCGGTCGTG 
ACGGGCCCCG AAATGAACCG CTTCCTCCTC GGCGAACGAG GCATCCAACT CTTTGAGGAC 
CTCGACCTCA AGCTCTCCGC TCCCGTGAAA TACGCTCCCT ACATGCCACC CCAACCTCCA 
CCGGACCCCT GGCACGAGGC CCGCATGCGC CTGACCATCC AGTTCCCCGA CAAGCGTCTC 
CTCCAATACG ACTGCGGCAA GCTCCAAGCG CTCGACAAGC TTCTACGCAA ACTCCAGGCG 
GGCGGCCACC GCGCTCTGAT CTTCACGCAA ATGACCAAAG TCCTCGACAT CCTCGAACAG 
TTCCTCAACA TCCACGGACA CAAGTACCTC CGTCTCGACG GCGCCACCAA GGTCGAACAG 
CGACAGATCC TCACAGACCG ATTCAACCAC GACCCGCGCA TCCTCTGCTT TATCCTGTCC 
ACCCGCTCCG GCGGTCTCGG CATCAACCTC ACGGGCGCCG ACACAGTCAT CTTCTACGAC 
CAGGACTGGA ACCCAGCCAT GGACAAACAA TGTCAAGACC GCTGCCATCG TATCGGTCAG 
ACGCGCGACG TACACATTTA CCGACTAGTA TCAGAACACA CCATCGAAGC GAACATTCTC 
CGCAAAGCTA GTCAAAAACA AATGTTGGAC GACGTGGTCA TCCAGGAAGG AGAGTTCACC 
ACCGATTACT TCAATCGGTT GTCGGTGCGA GACGTACTAG GGTCCAATGG CGAGGTGATC 
GCCAGCAACG AAGACGACGT GGCCGCCAAC CTCGCCATGG ATCGCGTGCT AGGTGGACCG 
TCGACTACCG GCGCAGGAGG CTACGACGGC ACTGCCGACG GCGGCGGCGG TGCATCCCAA 
CCCCCTGTGC GAAACGTGGG CAGGGTCCTA GAGATGGCAG AAGACAGAGA GGATGTCGAC 
GCTGCCAAGG CAGCAGAAAA GGAAATCATG CAGGACGAAG CAGATTTTGG CGAGGCGGGA 
AGTACGCGCC CGGGAACGCC GGGTGATGGG CTGGCGGATT TGGATGGTCA ACTTTTGGGA 
GGAGAAGAGA ATAAGGAGGT AGAGGATGAG GTGATTGAGT ATAATGCGTG GGGCGAGAGG 
ATGCATACTA TTGATGAGTA CATGCTGGGG TTCATGGCCA AGGCGTTGGA AGGGACGCCG 
TTGGAGTTAC CGCGGGACCG CAAGAAGGGA AGGGATAGGA ATAGGAATCG GAAGGGGAAG 
GATAGCAGGA AGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_958302.1
CDS	404..5941
Translation	MTTMMTDSGTASDSGAGLVDSTRNDTTTTTTTTTTPGDNDADDDNTNGTTTGHHDNNETDNNSKSYSSTHHVPAIDNTSTTNANDNEDAAGFLDRDQSPLSSISSPLSEPDNFEFDTEPIPSILSPKSTTSDNHARDGETPELDEDGQPPAKRRRVRETTPHNHSRKPKPESPPWKKFEAEGPTTIITEDGRRKSGRVNPVLLGMKPSDKKVTRKAIQTSPVSNKSSASTSRKPAPASSSNSKHAPAKMPPPPPPPKAPARKSATTHETRPSASRSRRRSPSPRRPATPPKPAAVGTRRSTRQALAHSRASYDDGQLSPGASRATPRIKLKVRPPLTVIPLVHPNQANVRPKLGPTFEEYFARAHEIPVEEGGQHIPDEEPKYTDEMALQDAKVILRVEKEVEPGGLLAPDRCSAFEPEAEEEPPRQWAHLDHLTKAMTNFRKLMYREQQLHMQAAKRIAIACEAEWRRRNPQPKTAEEIELEEMEASKAKWRQVIRAFAGTWENVRVEVNRRRLVEWEAEEQRRVKAALNQAVNLSEQKLQQRQAQVDGDEITDEDEDEDDEDLASGMPSVMGDEKESDEHSDQGSDEMSDENDEDEDEDNMSSSEDDDKKSTASDEGLTQEELRAKYANLPTLGTTDETEETTKDVEMADAPAAMDGSVANDDDTSDESVDMDDDLGSSEESDDDEEEEEDDEEEEESDDEPAGLLGLFFGKSELKKLKEEAVTEEPSVEPAGDVEMTDASAALRPELQVNGHAHEAPITNGTHTNEQLASSQTEGADDEVSLLVIPNDEIAAENTQTAAEPGSGVVEKDFLTNDSSLKYPNEIVQSENQTLPAKESVEAGGDLPMVDAPSTDDLQRETAAAPSLEAPPNTRHDSQETVAATDMQSQSQTQSPKTTDTKPTDVDTPHSELAVSVQKPDSRQSSPQPTTPTVKTEIPFLLRGTLREYQHHGLDWLAGLYANNTNGILADEMGLGKTIQTIALLAHLACHHEVWGPHLVIVPTSVMLNWEMEFKKWCPGFKILTYYGNQEERKRKRQGWNNDDVWNVCITSYQMVLQDQQVFRRRRWHYMILDEAHNIKNFKSQRWQTLLGFNTQARLLLTGTPLQNNLTELWSLLYFLAPPENGEGGFVDLTEFHNWFARPESQILESGREQLDDEARAIIAKLHKVLRPYLLRRLKADVEKQMPAKYEHVEFCRLSKRQRELYDGFLSRADTRETLQSGNYMSIINCLMQLRKVCNHPDLFVDRPIMTSFRMSRSVPADYEWKEKFIRNRLLATKPMTTVNLSFLNMIPTEYEDLSKTHTDRIAQLSSHRILLDLREAQKVRANNAYTALDPSSVKSNLVYLESAARWGRFEELQHCVYINALRRQQRPIYGKRLTEFLTLDTHLRPYKPRPRVPAKIMSWFEEDSFLLHNAIPTLQQRAESMEMTITKFACVTPAVVTGPEMNRFLLGERGIQLFEDLDLKLSAPVKYAPYMPPQPPPDPWHEARMRLTIQFPDKRLLQYDCGKLQALDKLLRKLQAGGHRALIFTQMTKVLDILEQFLNIHGHKYLRLDGATKVEQRQILTDRFNHDPRILCFILSTRSGGLGINLTGADTVIFYDQDWNPAMDKQCQDRCHRIGQTRDVHIYRLVSEHTIEANILRKASQKQMLDDVVIQEGEFTTDYFNRLSVRDVLGSNGEVIASNEDDVAANLAMDRVLGGPSTTGAGGYDGTADGGGGASQPPVRNVGRVLEMAEDREDVDAAKAAEKEIMQDEADFGEAGSTRPGTPGDGLADLDGQLLGGEENKEVEDEVIEYNAWGERMHTIDEYMLGFMAKALEGTPLELPRDRKKGRDRNRNRKGKDSRKR

Target ORF information:

RefSeq Version	XM_953209.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A helicase swr-1 (crf1-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_953209.2

ORF Insert Sequence:

ATGACTACTA TGATGACCGA CTCTGGCACC GCGTCTGATT CTGGTGCGGG ACTTGTTGAT 
TCAACACGAA ACGACACCAC CACCACCACC ACCACCACCA CCACTCCCGG TGACAACGAC 
GCCGACGACG ACAACACCAA CGGCACTACT ACTGGTCATC ACGACAACAA CGAGACAGAC 
AACAACAGCA AAAGTTACAG CAGCACCCAC CATGTCCCCG CCATCGATAA TACCTCGACG 
ACCAACGCCA ACGACAACGA AGATGCTGCT GGCTTCCTCG ACCGCGACCA GTCGCCCTTG 
TCGTCCATCT CGTCCCCGCT GAGCGAACCC GACAACTTTG AGTTTGATAC CGAACCGATT 
CCATCCATCC TATCACCAAA GTCTACGACA AGCGATAATC ATGCCCGCGA CGGAGAGACA 
CCCGAGCTCG ACGAAGATGG ACAACCACCG GCCAAGCGAC GCCGAGTGCG CGAGACAACA 
CCTCACAATC ACTCGCGCAA ACCGAAACCA GAGTCACCAC CATGGAAGAA GTTTGAGGCC 
GAGGGTCCGA CTACCATCAT CACCGAAGAC GGTCGTCGCA AGTCTGGCCG CGTCAATCCC 
GTCTTGCTAG GCATGAAACC CAGCGACAAA AAGGTTACGC GGAAAGCTAT CCAAACAAGT 
CCTGTCAGCA ACAAAAGTAG TGCCAGTACG TCCAGGAAGC CAGCGCCAGC AAGCAGCAGC 
AACAGCAAAC ATGCTCCCGC AAAGATGCCG CCCCCGCCGC CGCCGCCGAA AGCTCCCGCG 
CGCAAGTCTG CCACGACTCA TGAAACCAGA CCCTCCGCGT CCCGATCCCG TCGACGATCT 
CCATCTCCTC GACGTCCTGC TACACCACCG AAACCCGCCG CTGTCGGTAC GCGTCGGTCA 
ACGCGCCAAG CCCTAGCCCA TTCTCGAGCA TCCTACGATG ATGGCCAGCT CTCTCCCGGT 
GCCTCGCGCG CCACTCCCCG TATCAAGCTC AAAGTTCGAC CCCCGTTGAC CGTAATACCT 
CTTGTTCATC CAAACCAAGC CAATGTTCGT CCAAAGCTTG GGCCGACCTT TGAGGAATAC 
TTTGCGCGCG CTCATGAAAT ACCGGTTGAG GAGGGTGGGC AGCACATTCC CGATGAAGAG 
CCCAAATACA CAGACGAGAT GGCCCTCCAA GACGCCAAGG TCATTCTACG GGTTGAAAAG 
GAGGTTGAGC CAGGGGGCTT GCTCGCCCCT GATAGGTGTT CGGCTTTCGA GCCTGAAGCC 
GAGGAGGAAC CGCCTCGACA GTGGGCTCAC CTTGACCATC TCACAAAGGC CATGACCAAC 
TTCCGCAAGT TGATGTATAG AGAGCAGCAG TTGCATATGC AGGCAGCCAA GCGCATCGCC 
ATTGCATGCG AAGCCGAGTG GAGGCGCCGA AACCCACAGC CCAAGACTGC CGAAGAAATC 
GAGCTGGAGG AGATGGAAGC GTCCAAGGCC AAATGGCGCC AGGTTATCAG GGCCTTTGCT 
GGAACATGGG AGAATGTCAG AGTCGAGGTC AATAGGCGCC GCTTGGTGGA ATGGGAGGCG 
GAGGAACAGC GCCGTGTCAA GGCCGCGCTG AATCAAGCAG TCAATCTTTC GGAACAAAAG 
CTACAGCAAC GACAAGCGCA AGTGGACGGG GACGAAATCA CAGACGAGGA TGAGGACGAG 
GACGACGAAG ACCTGGCAAG TGGCATGCCG TCAGTAATGG GCGACGAGAA GGAAAGTGAC 
GAGCACTCCG ACCAAGGATC CGATGAGATG TCCGACGAAA ATGACGAAGA TGAAGACGAG 
GATAACATGT CCTCCTCCGA GGACGACGAT AAGAAATCGA CCGCCTCGGA CGAGGGCCTG 
ACTCAGGAAG AATTGCGTGC AAAATACGCC AACCTACCCA CATTGGGCAC GACCGATGAA 
ACTGAAGAAA CAACAAAAGA CGTGGAAATG GCTGATGCTC CCGCTGCTAT GGATGGTAGC 
GTTGCGAATG ATGACGATAC AAGCGATGAG TCGGTAGACA TGGACGACGA CCTTGGATCT 
AGCGAGGAGT CAGACGACGA CGAGGAGGAG GAAGAAGACG ATGAGGAAGA GGAAGAGTCC 
GATGATGAAC CTGCTGGCTT GCTAGGGCTA TTCTTTGGAA AGTCAGAGTT GAAGAAGTTG 
AAGGAAGAGG CTGTAACTGA GGAGCCGTCG GTTGAACCCG CTGGGGATGT GGAGATGACT 
GATGCTTCGG CGGCCCTTCG TCCCGAGCTC CAAGTTAATG GTCATGCGCA TGAGGCCCCG 
ATAACTAATG GTACTCACAC TAATGAGCAG TTGGCATCAT CTCAAACTGA GGGTGCAGAC 
GATGAAGTGT CGTTACTGGT TATACCCAAC GACGAAATAG CAGCAGAAAA TACTCAAACC 
GCAGCAGAGC CGGGCAGCGG AGTGGTGGAG AAGGACTTCC TGACCAATGA TAGTTCCTTG 
AAGTATCCTA ATGAGATTGT ACAGTCCGAA AACCAGACAC TTCCGGCCAA GGAGTCGGTT 
GAAGCTGGAG GCGACCTGCC AATGGTTGAT GCCCCATCCA CAGACGATTT ACAAAGGGAA 
ACAGCCGCGG CGCCGTCGTT GGAGGCACCA CCCAACACCC GACATGATAG TCAGGAAACG 
GTGGCGGCTA CTGACATGCA GAGCCAGTCA CAAACACAGT CACCAAAAAC AACTGATACC 
AAGCCCACCG ACGTCGACAC TCCCCATTCA GAGCTGGCCG TCAGCGTGCA AAAGCCAGAT 
AGCCGCCAAT CCTCACCTCA GCCAACAACA CCAACAGTCA AGACCGAAAT CCCGTTTCTC 
TTACGTGGAA CACTGCGTGA GTATCAACAT CATGGTCTGG ACTGGCTTGC TGGGCTGTAT 
GCCAACAACA CCAATGGTAT CCTGGCCGAC GAGATGGGTT TGGGAAAGAC CATCCAGACG 
ATTGCTCTGC TGGCGCACCT CGCCTGTCAT CATGAAGTTT GGGGCCCCCA TCTGGTTATT 
GTGCCTACGA GTGTTATGCT CAACTGGGAA ATGGAGTTCA AGAAGTGGTG TCCTGGATTC 
AAGATCCTTA CGTACTACGG CAACCAGGAA GAGCGAAAGC GAAAGCGCCA GGGATGGAAC 
AACGACGATG TGTGGAACGT CTGCATCACC TCCTACCAAA TGGTCCTTCA GGACCAACAG 
GTGTTTAGGC GAAGACGGTG GCATTACATG ATCCTCGACG AGGCTCACAA CATCAAGAAT 
TTCAAGTCGC AGCGCTGGCA AACCCTGCTC GGCTTCAACA CGCAAGCTCG GTTACTTTTG 
ACAGGTACTC CGTTACAGAA CAACCTGACC GAGCTGTGGT CGCTTCTTTA CTTCTTGGCG 
CCACCCGAGA ACGGCGAAGG CGGGTTCGTT GACCTCACCG AATTCCATAA CTGGTTCGCT 
AGGCCCGAAT CTCAGATCTT GGAAAGCGGG CGTGAACAGC TGGACGACGA GGCGCGTGCT 
ATCATTGCCA AGCTGCACAA GGTCCTTCGA CCCTACCTTC TCCGTCGACT CAAGGCCGAT 
GTCGAGAAAC AGATGCCGGC AAAGTATGAA CACGTCGAGT TCTGCCGCCT CTCCAAACGT 
CAGCGAGAGT TGTATGATGG GTTCCTCTCC CGTGCCGATA CACGCGAGAC GCTGCAGTCG 
GGTAACTACA TGTCCATCAT TAACTGTTTG ATGCAGCTCC GAAAGGTCTG CAACCATCCC 
GATCTCTTTG TGGACCGGCC CATCATGACC TCGTTCCGCA TGTCGCGGTC AGTACCGGCC 
GACTACGAGT GGAAAGAAAA GTTCATTCGG AACAGGCTGT TGGCTACGAA GCCCATGACC 
ACTGTCAATT TGAGCTTCCT CAACATGATT CCGACCGAGT ACGAAGATCT GTCCAAGACA 
CACACCGACC GCATCGCCCA GCTCAGCTCC CATCGTATCT TGCTCGACCT GAGGGAGGCC 
CAGAAGGTGC GCGCCAACAA CGCCTACACC GCGCTGGACC CTTCCTCTGT CAAGTCCAAC 
CTTGTGTATC TTGAAAGTGC CGCCAGGTGG GGTCGGTTCG AAGAGCTGCA GCACTGTGTA 
TACATCAATG CCCTCCGTCG CCAACAACGA CCCATTTATG GAAAGCGTCT AACCGAGTTC 
CTCACCCTTG ATACCCATCT CCGACCATAC AAACCCCGAC CTAGAGTGCC GGCTAAGATC 
ATGTCCTGGT TCGAAGAGGA TTCCTTCCTG CTACACAACG CCATCCCAAC GCTCCAGCAA 
CGCGCCGAAT CCATGGAAAT GACCATCACC AAGTTCGCCT GCGTCACTCC CGCGGTCGTG 
ACGGGCCCCG AAATGAACCG CTTCCTCCTC GGCGAACGAG GCATCCAACT CTTTGAGGAC 
CTCGACCTCA AGCTCTCCGC TCCCGTGAAA TACGCTCCCT ACATGCCACC CCAACCTCCA 
CCGGACCCCT GGCACGAGGC CCGCATGCGC CTGACCATCC AGTTCCCCGA CAAGCGTCTC 
CTCCAATACG ACTGCGGCAA GCTCCAAGCG CTCGACAAGC TTCTACGCAA ACTCCAGGCG 
GGCGGCCACC GCGCTCTGAT CTTCACGCAA ATGACCAAAG TCCTCGACAT CCTCGAACAG 
TTCCTCAACA TCCACGGACA CAAGTACCTC CGTCTCGACG GCGCCACCAA GGTCGAACAG 
CGACAGATCC TCACAGACCG ATTCAACCAC GACCCGCGCA TCCTCTGCTT TATCCTGTCC 
ACCCGCTCCG GCGGTCTCGG CATCAACCTC ACGGGCGCCG ACACAGTCAT CTTCTACGAC 
CAGGACTGGA ACCCAGCCAT GGACAAACAA TGTCAAGACC GCTGCCATCG TATCGGTCAG 
ACGCGCGACG TACACATTTA CCGACTAGTA TCAGAACACA CCATCGAAGC GAACATTCTC 
CGCAAAGCTA GTCAAAAACA AATGTTGGAC GACGTGGTCA TCCAGGAAGG AGAGTTCACC 
ACCGATTACT TCAATCGGTT GTCGGTGCGA GACGTACTAG GGTCCAATGG CGAGGTGATC 
GCCAGCAACG AAGACGACGT GGCCGCCAAC CTCGCCATGG ATCGCGTGCT AGGTGGACCG 
TCGACTACCG GCGCAGGAGG CTACGACGGC ACTGCCGACG GCGGCGGCGG TGCATCCCAA 
CCCCCTGTGC GAAACGTGGG CAGGGTCCTA GAGATGGCAG AAGACAGAGA GGATGTCGAC 
GCTGCCAAGG CAGCAGAAAA GGAAATCATG CAGGACGAAG CAGATTTTGG CGAGGCGGGA 
AGTACGCGCC CGGGAACGCC GGGTGATGGG CTGGCGGATT TGGATGGTCA ACTTTTGGGA 
GGAGAAGAGA ATAAGGAGGT AGAGGATGAG GTGATTGAGT ATAATGCGTG GGGCGAGAGG 
ATGCATACTA TTGATGAGTA CATGCTGGGG TTCATGGCCA AGGCGTTGGA AGGGACGCCG 
TTGGAGTTAC CGCGGGACCG CAAGAAGGGA AGGGATAGGA ATAGGAATCG GAAGGGGAAG 
GATAGCAGGA AGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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crf1-1 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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