pks-1 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	pks-1
Entrez Gene ID	3874282
Full Name	polyketide synthase-1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_953042.3	XP_958135.3	polyketide synthase 1

Pathways from BioSystems

Homologies

Neurospora crassa	NCU06013	XP_958135.2
Danio rerio (zebrafish)	LOC559001	XP_687387.4
Danio rerio (zebrafish)	fasn	XP_005169535.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009176	XP_319941.4
Magnaporthe oryzae (rice blast fungus)	MGG_05589	XP_003710466.1
Drosophila melanogaster (fruit fly)	CG3523	NP_608748.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pks-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pks-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf10645	XM_953042.3 Latest version!	Neurospora crassa OR74A polyketide synthase 1 (pks-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pks-1 ( XM_953042.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_958135.3 Neurospora crassa OR74A polyketide synthase 1 (pks-1), partial mRNA.

Price & Availability↑

CloneID	ONf10645
Clone ID Related Accession (Same CDS sequence)	XM_953042.3
Accession Version	XM_953042.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	polyketide synthase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506). On Feb 23, 2015 this sequence version replaced XM_953042.2. COMPLETENESS: incomplete on the 5' end.

ATGGCGCCCA TTGCTGTGAC GCCGGATAGC TCAACCGCCA GTCCTTCTGG CGACAGCTGG 
AACAGTTACT TTCACGTGCG TAATGGTGAC AGCCATAACA TCATCAGCAA CAAGGGCCAT 
GATCATGGCG AAAACGCCCT CTCGGCAGCC GTAGGGAATG ACCATGGCAG CTGGACTGCC 
GAACCTGGGA TCGGCTTGAG TTCCCCTCTC CCTCCCCCGG GCCATGTTCG AGATATGCCC 
ATAGCCATTG TTGGGATGGC CTGTCGGATG CCTGGCAATG TCTCTACTCC GGCCGAATTC 
TGGGAGCTCT GCACTAGGGC CCGGTCTGGC TGGTCCGAAA CTCCCAAGCA GAGATTCAAC 
AGTGCCCGCT TCCATCATCC CAACCAGGGC AAAGGCGGCA CTCTGAATCC CGTCGGCGGC 
AACTTCTTGA ATGTCGACCT GGCTGCCTTT GATGCTCCAT TTTTTGGTTT GACTGAAAAG 
GAGGCCATTT CCATGGATCC CCAGCAGCGT CTTCTTCTCG AATGCACCTT TGAAGCCCTC 
GAAAATGCTG GCATACCAAA GCATACCATT GCCGGAAAGG ACGTTGGCGT TTTTGTCGGC 
GGTTCTTTTC CCGAGTACGA ATCTCATCTC TTCAGAGACC CAGATACCAT ACCTATGCAC 
CAGGCGACCG GTTGTGCATA TGCCATGCAG TCCAACCGCA TATCCCACTT CTTCGACCTA 
CGAGGCCCCA GTTTCACGGC CGATACAGCA TGCTCCTCTA GCATGGTAGC CATACACCTG 
GCTTGCCAGA GCTTGCGAAC CGGTGAATCT AGCGCCGCTT TGGTCGGCGG CTGTCACCTC 
AACATGCTTC CCGAGTTCTG GATCTCCTTT TCGACTTGCA GACTGCTCTC GGATGCCGGC 
CGTTCATTTT CTTTTGACAA TCGCGGCACG GGGTTTGGCC GTGGAGAGGG CTGTGGCATG 
ATTGTCCTGA AGCCACTGGA CCAAGCCATA AAGGACAAGG ATCCCATCAG GGCAGTCATT 
GCCGCAACTG GTCTCAACCA AGATGGCAAG ACGCCCGGCA TCACTGTGCC ACATGGGCCA 
GCGCAAGAGG ACCTCATACG CAAAGTATAT GGCAGGGCTG GCCTCGACCC GAATTTGTGC 
GGCTTCGTCG AGGCCCATGG AACTGGCACT AGGGTTGGTG ATCCCATCGA AGCCACTGCC 
ATTCACAATG TTTTAGGTCA AGAGAGAAGC CCACGCAACC CCCTATGGAT CGGCTCTGTG 
AAATCCAACA TTGGCCATCT GGAAGGGGCC TCCGGTATTG CCGGTGTCAT TAAAGCAGCC 
CTTATGCTTG AGCGGGGCTT CATCCTTCCA AACCATGATT TTAAGCAGCC AAACCCGAGG 
ATCCCCTGGA AAGAATGGAA TTTAGAGGTC CCGGTGACCC AGAGGCCGTG GCCGCGCGGC 
AAAAAGTACA TCAGTGTCAA TAATTTTGGT TTTGGTGGCA CAAATGGCCA TGTTGTACTG 
ACAGCCGCAC CATTCCTGCG TTCCAGAACT TCGGGGTCCT CCCATGATGA CACCCTTATG 
GCTTCGGATA AGTCAGGGCT GGGCCGCAAG CTTTACGTCG TCACGGCTCA TGACAAGGCC 
GCCCTTTCGC AACAGATGAA GAATATCGTG GTCTACCTTG AGCAGAGGCC CGAGATCTTC 
CAAGTAGATC TAACCAGCAA CCTTGCTTAC ACGCTTGGGC AGCGGCGGTC CCTCCTGCAA 
TGGAGAGCTG CCATTCCCGC TATCAACTCA TTTGAGTTAA TCGAAGCTAT CAATGGAGAA 
AAGGCGATAA CCGGTAAAGA AATCGAGCCT CTTAGACTTG GTTTTATTTT TACCGGTCAG 
GGTGCTCAAT GGTATGCCAT GGGTCGGGAA TTGTACGAGC AGTATCCAAC GTTCAGTGCG 
AGTATTTTGC AGGCTGATGC CTGTTTAATC GCTTTCGGGG CGGAATGGTC ACTTGTTGAG 
GAACTCGGAA GAGATGCCAA AACCAGCGGG ATCAGTGAGG CACACATTAG CCAACCATCA 
TGTACTGCAG TACAACTAGC ACTTGTTAAC TTGCTACGAG GCTGGGGCCT GCGGCCAACT 
GCTGTTGCAG GCCACTCGAG TGGTGAGATC GCTGCTGCAT ATGCTGCGGG CATCATTGAA 
TTTGAATCTG CCATGGCAAT TGCCTATCAT CGAGGTAGAC TGATTCCTAT CCTCAAAACG 
AAGTACCCTG AGCTTCAGGG TCGCATGATG GCGGTTGGAG GAAGCAAGAA GGAATTCACA 
ACCCTTATCG AAGGACTACA AGCGAAGGAA GTCCGAATTG CTTGCTACAA TAGTCCTTCC 
AGTTTAACTA TTTCCGGCGA TGAACCTGCC TTGGCCGAAT TGGAGAGGAT TTGCGAGAAG 
AGGCAGCTGT TCAACAGGCG TCTGGTCGTC GACGTCGCTT ACCATTCCCA CCACATGAAC 
TTGGTCGCCA AGGATTACCG TGCTTCCATC GCCAACCTTC AGCCGCCAGC TCGAACTGAC 
GTGCAGTTTC ACTCTTCTCT GTATGGCCAT CCTGTTGACA GTACCGATTT GCAGCCTAAC 
TACTGGGTTG ACAACTTGAC TTGTCCTGTA CGTTTCAATG AGGCGCTGCA GAGCATGCTT 
GCGCCGGTCG GCGAGCATAA GCACGGGGTC AACATGCTTG TCGAGCTCGG CCCCCATTCC 
GTGCTTCAAG GACCAGTCAA GCAGATACTC AAAGAGGTCG GTGGAAGTGC ACCAAAAATT 
CCATATGCTT CTGTTCTTGT TAGAAAACGA AACGCCGTCG AGACCGCTCT GGAACTTGCC 
GCACAGTTGT TCACCAGAGG GGCAAACCTT GATCTTGGTG CCGTCAATTT TCCAAAGACA 
ACCAAACCAC CAACGCTGCT GACAGATTTA CCACGGTATC CCTGGAACCA TTCAATCAGG 
TATTGGCAAG AATCTCGTAT GACAGATGTG CATAAGAACG CGGTTGGTTC AAGGAGCGAC 
ATTATCGGTA CCTTGGCCAG CTACTCGAGC GATTTAGAGC CCACATGGCG CAACATCGTT 
CGTCTGGACG ACCTGCCCTG GCTGCGCCAT CACAAAATTC AATCCTTGAT CATGTATCCC 
ATGTCCGCAT TTCTTGCGAT GGCTGTGGAG GCCGCCTCCC AGAGGGCGAG AAGCAAACAC 
TGCTCATTCG ATAGGTTTCA GATGAGAGAT ATTGCCATAA AAAAGCCCCT AGCGATACCA 
GACAACGACA TCGAGGTTAC AATCAGTCTC CGTCCGCACC AAGAGGGTAT CTTTGTTTCA 
TCCGAGACGT GGGACGAATT CAGGGTTTGC TCATGGTCTT CTGGTCAAGG TTGGATAGAG 
CATTGCGTCG GTTTGATCGC GACGGTGAAA ATTACAGAAA ATGATGTGGT ACAGGGCCCA 
CAGACTGATG ATGGCTTTGA AGACCACCTA CATTCTGTCG TCCCCACCAT CGACAACGCA 
CAATGGGCCT CGATCGATCC CCAGCATATG TACGAGACCC TCAACAAGCT TGGGGTTGTG 
TACGGGCCTA CCTTTCAAGG TCTCGACAAT ATCAACGTGT GCGATACACA GTCAACAGGA 
GATCTTGTTG CTCCAGATGT CGCCAAAGAG ATGCCGGGTG GGTATTTGAC CAAAGCCGTG 
ATACAGCCCG CATTTCTCGA GTCGCTCATT GGCATGTACT GGCCGGTACT TGGAGCCGGA 
CGCCGGCAAG TTGACACGAT TTACCTACCG TCCGCGATCG AGTACGTGAC CATATCTCGA 
AGAATAACCC AACTGGCAAG CGAGCCAGGA AATACGCTGC GCGCCATTTG CAAGGGCGAG 
GTCGATCCTG GCATTCATCG GCCGTTCAAG GTTCACATGG TTGCGGCCCC CGCGGGTTCT 
CTGGAGAGCA TAATTGCAAT CAATGGCTTG ACAGTCTCTC CTGTGCTGAA CGATGATACT 
CGAGAGAAAA ACAACACTCC CCGTGACCTC TGCTACAAAC TTGAATGGGA ACACATCTTG 
GAGCCTGAAC ATGTGAGAAA CCAGCTCCCT GTCGGTGCCC AAATTGTGAT CATTCACGAG 
GATTCAAACT TTCAGTTGCT TGTCGCTCTG AGGTTGGCGA ACGCACTTGA GCAAGCCACG 
GGGAGATTAC CTGAAGTCGG TAAGCTTGAC ACAGTTGATG CAAAAGGAAA GACTTGCATC 
TTCCTAAATG AGCTGCATCG TCCTCTCCTG GCCACCCTTT CGCCATGGCA GTTCTCTAGC 
TTACAAAAGC TTTTGACCGG GGTTGAAGCC ATTCTGTGGG TAGTCAAGGG AGCCTACGAC 
AAGTGCACCG AACCGGAAGC ATATATGGTT ACCGGCCTGA GCAGGTCCAT CCGTTCGGAA 
ACTGCACTTC GGTTTGCCAC ACTGGATCTA GATGCGGCCT CTCCCCTCTC GGAAGCCAAG 
GCCGCAGAGA TGGTTTTTTG CGTCTTCAAC ATGGCTTTTG GGTCTGCTTC TTCGTCGATG 
AGTGAACTGG AGTTTATGGA GAGGGGAGGA TCGCTTTTCA CTCCGCGTAT CACCCATGAC 
AGAGAAACTG ACCAAGAAGT GCAAACCTTG ACAAATCCAA GAGCCTTAGA GCCAACCGCT 
TTTGGGGACG ACGGCCGACT TCTGAGGCTA GAGATATCTG CGCCCGGTGC TTTACATACA 
CTTCACTTCG TCGACGATCA CTCACTGGAA GTGCCACTAG CAATTGATCA ACTTGAGATT 
GAGGTCAAGG CTGTCGGCTT GAGTTTCCGG GACGCCATGG CTGCAAAGGG CCAAATTCCC 
ATCACCATGT CGGGTGCTGA AGCAAGCGGG ATTGTCGTCG CTGTTGGGAG CAGTATCTCC 
GCTTTCAAGG TTGGCGATCG CGTTGCAGCG CTCACGCAAG GCGCTTTTGC GACACGAACG 
AGGGCAACTG CAAGCTTAGC AATCAAGGTC CCAGGACACA TCTCCTTTGA AGCAGCTGCC 
ACACTGCCCC TTGGATACTG CGTTGCATAC TACAGCCTGG TTGATGTCGG GCGCTTGGCT 
GAGGGAGAAA CAGTTCTCAT TCACCAAGCA GCAGGGGCCG TCGGTCAAGC AGCCATTTGC 
CTCGCTCAGA TGATTGGAGC TGAGATTTTC GCAACGGTTG GGAGCGCTGA GAAGAAGGAG 
CTGCTCATTA ATGAGTTCAG TCTTCCTTCC GATCATGTCT TGTACAGTCG CGACACTAGT 
TTTGGGCCTA CTATCCGCCG TGCTACATAT GGCAAGGGGG TAGATGTTAT CCTCAACTCG 
CTGGTTGGCG ATGGCCTACG GGAATCCTGG AAATCTCTCA GCAAGTTTGG ACGGCTCGTG 
GATATAGGCA AGCGAGACTC TGCCTCAAAG TCTCGAACCG AAGTATCCGC TGCCAAGAGA 
AACGCTAGCT TTGTTTCTGT TGACATCTTT GCTCTGTTGG CCGAACGGCC AAAGGCTCTC 
AAACGCGTGT TTGCCGATGT TGCCCGACTT CTCAGATATG GAAAGGTTCG GTCGGCGAGC 
CCGCTATCTG TCTTTCCGGT TTCGGATATT GAGGGCGCAT TGAAGATGCA ACAAAGCGGC 
TCTCCTACTA TGGGCAAGGC AGTCGTGGCT CTAAGTGGGT CTGATTTTGT CAAGGCCCCG 
CCATCACGTC GGTTAAAGGA CCTCCTTCAT TCCGATGCAA CATATATCTT GGTTGGCGGC 
ACCGGTGGTC TCGGAAGGAG TATTGCGCGA TGGATGGCAG GAAAGGGGGC GCAAAACATC 
GTTCTTGTCT CGAGAAGCGC TTCCGTTACC GGCAAGGTCA AGGAACTTGC CGAAGACCTC 
TCTATGATGG GGGTGAGCGT TCATGTCCGC CGCTGCAATG TGGTCAACGA GACTGAAGTG 
GACGAGCTCA TTCGAACTGG GCTACAGGGG CTACCTCCTA TTAGGGGTAT TGTGCTCGGC 
ACGATGGTAT TACACGACGT TCTTTTCGAG AAAATGACAT GGACTGACTA TACCGAAGTC 
ATCAAGGGAA AAGTTCAAGG TGGTTGGAAC TTTCACCGTG CATTGTTGAC CTCGCCACTT 
GATTTCTTCG TGGCCATTTC TTCCGCAGCT AGTGCCGTGG GGAACCGTGG GCAAGCGGCA 
TATAGTGCCG CAAACTGCTT TCTGAATTCT TTGGTCCAGT ACCGGCTTAC CCTTGGTCTT 
CCCGCGTCAT CTCTTGACCT CACCGCTGTT TCCGACTCCG GCTATCTGGC TGAAGACCCG 
GAAAAGGCTG CTGAGGTAGC CCGGAATCTT GGTAGCGATA CGATATGCGA GGCAGAAGTC 
CTCGCCCTGT TGCATGCCGC CATTAGTGGA AAGCTCACGT TGGCCTGCAA CAATCATACG 
ATTACCGGCA TGCGTATTAC CTCGACAATG CGACCGTTCT GGACCGATGA TGCAAAATTC 
AAGACCATGC GCATGGCAGC AGAGGATGAG GCTGCAAGAA ATGCATCATT GAACATGGTT 
GTGTCGTTCA GTGCAACTTT GAAGAATGCC AAATCACTCA TCGAGGCCGA AGACGTCGTC 
TGCAAAGGAC TGGTAGAGAA GATTTCATCT GTGTTAATGC TTGATGCGGA GGATATGGAT 
ATCACACGAT CTCTCTCACA CTATCCCCTT GATTCTTTGG TTGCTATCGA GATCCGCAAC 
TTCATTACTC GTGACTTTGA AGCGAATCTT CAGGTGCTGG AGCTTCTTTC AAGCGGGTCG 
ATTCAAACGT TAGCAAAGGC GGTATGTGCA AAGTCTAAGA TCGTCTCATT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_958135.3
CDS	1..6774
Translation	MAPIAVTPDSSTASPSGDSWNSYFHVRNGDSHNIISNKGHDHGENALSAAVGNDHGSWTAEPGIGLSSPLPPPGHVRDMPIAIVGMACRMPGNVSTPAEFWELCTRARSGWSETPKQRFNSARFHHPNQGKGGTLNPVGGNFLNVDLAAFDAPFFGLTEKEAISMDPQQRLLLECTFEALENAGIPKHTIAGKDVGVFVGGSFPEYESHLFRDPDTIPMHQATGCAYAMQSNRISHFFDLRGPSFTADTACSSSMVAIHLACQSLRTGESSAALVGGCHLNMLPEFWISFSTCRLLSDAGRSFSFDNRGTGFGRGEGCGMIVLKPLDQAIKDKDPIRAVIAATGLNQDGKTPGITVPHGPAQEDLIRKVYGRAGLDPNLCGFVEAHGTGTRVGDPIEATAIHNVLGQERSPRNPLWIGSVKSNIGHLEGASGIAGVIKAALMLERGFILPNHDFKQPNPRIPWKEWNLEVPVTQRPWPRGKKYISVNNFGFGGTNGHVVLTAAPFLRSRTSGSSHDDTLMASDKSGLGRKLYVVTAHDKAALSQQMKNIVVYLEQRPEIFQVDLTSNLAYTLGQRRSLLQWRAAIPAINSFELIEAINGEKAITGKEIEPLRLGFIFTGQGAQWYAMGRELYEQYPTFSASILQADACLIAFGAEWSLVEELGRDAKTSGISEAHISQPSCTAVQLALVNLLRGWGLRPTAVAGHSSGEIAAAYAAGIIEFESAMAIAYHRGRLIPILKTKYPELQGRMMAVGGSKKEFTTLIEGLQAKEVRIACYNSPSSLTISGDEPALAELERICEKRQLFNRRLVVDVAYHSHHMNLVAKDYRASIANLQPPARTDVQFHSSLYGHPVDSTDLQPNYWVDNLTCPVRFNEALQSMLAPVGEHKHGVNMLVELGPHSVLQGPVKQILKEVGGSAPKIPYASVLVRKRNAVETALELAAQLFTRGANLDLGAVNFPKTTKPPTLLTDLPRYPWNHSIRYWQESRMTDVHKNAVGSRSDIIGTLASYSSDLEPTWRNIVRLDDLPWLRHHKIQSLIMYPMSAFLAMAVEAASQRARSKHCSFDRFQMRDIAIKKPLAIPDNDIEVTISLRPHQEGIFVSSETWDEFRVCSWSSGQGWIEHCVGLIATVKITENDVVQGPQTDDGFEDHLHSVVPTIDNAQWASIDPQHMYETLNKLGVVYGPTFQGLDNINVCDTQSTGDLVAPDVAKEMPGGYLTKAVIQPAFLESLIGMYWPVLGAGRRQVDTIYLPSAIEYVTISRRITQLASEPGNTLRAICKGEVDPGIHRPFKVHMVAAPAGSLESIIAINGLTVSPVLNDDTREKNNTPRDLCYKLEWEHILEPEHVRNQLPVGAQIVIIHEDSNFQLLVALRLANALEQATGRLPEVGKLDTVDAKGKTCIFLNELHRPLLATLSPWQFSSLQKLLTGVEAILWVVKGAYDKCTEPEAYMVTGLSRSIRSETALRFATLDLDAASPLSEAKAAEMVFCVFNMAFGSASSSMSELEFMERGGSLFTPRITHDRETDQEVQTLTNPRALEPTAFGDDGRLLRLEISAPGALHTLHFVDDHSLEVPLAIDQLEIEVKAVGLSFRDAMAAKGQIPITMSGAEASGIVVAVGSSISAFKVGDRVAALTQGAFATRTRATASLAIKVPGHISFEAAATLPLGYCVAYYSLVDVGRLAEGETVLIHQAAGAVGQAAICLAQMIGAEIFATVGSAEKKELLINEFSLPSDHVLYSRDTSFGPTIRRATYGKGVDVILNSLVGDGLRESWKSLSKFGRLVDIGKRDSASKSRTEVSAAKRNASFVSVDIFALLAERPKALKRVFADVARLLRYGKVRSASPLSVFPVSDIEGALKMQQSGSPTMGKAVVALSGSDFVKAPPSRRLKDLLHSDATYILVGGTGGLGRSIARWMAGKGAQNIVLVSRSASVTGKVKELAEDLSMMGVSVHVRRCNVVNETEVDELIRTGLQGLPPIRGIVLGTMVLHDVLFEKMTWTDYTEVIKGKVQGGWNFHRALLTSPLDFFVAISSAASAVGNRGQAAYSAANCFLNSLVQYRLTLGLPASSLDLTAVSDSGYLAEDPEKAAEVARNLGSDTICEAEVLALLHAAISGKLTLACNNHTITGMRITSTMRPFWTDDAKFKTMRMAAEDEAARNASLNMVVSFSATLKNAKSLIEAEDVVCKGLVEKISSVLMLDAEDMDITRSLSHYPLDSLVAIEIRNFITRDFEANLQVLELLSSGSIQTLAKAVCAKSKIVSF

Target ORF information:

RefSeq Version	XM_953042.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A polyketide synthase 1 (pks-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_953042.3

ORF Insert Sequence:

ATGGCGCCCA TTGCTGTGAC GCCGGATAGC TCAACCGCCA GTCCTTCTGG CGACAGCTGG 
AACAGTTACT TTCACGTGCG TAATGGTGAC AGCCATAACA TCATCAGCAA CAAGGGCCAT 
GATCATGGCG AAAACGCCCT CTCGGCAGCC GTAGGGAATG ACCATGGCAG CTGGACTGCC 
GAACCTGGGA TCGGCTTGAG TTCCCCTCTC CCTCCCCCGG GCCATGTTCG AGATATGCCC 
ATAGCCATTG TTGGGATGGC CTGTCGGATG CCTGGCAATG TCTCTACTCC GGCCGAATTC 
TGGGAGCTCT GCACTAGGGC CCGGTCTGGC TGGTCCGAAA CTCCCAAGCA GAGATTCAAC 
AGTGCCCGCT TCCATCATCC CAACCAGGGC AAAGGCGGCA CTCTGAATCC CGTCGGCGGC 
AACTTCTTGA ATGTCGACCT GGCTGCCTTT GATGCTCCAT TTTTTGGTTT GACTGAAAAG 
GAGGCCATTT CCATGGATCC CCAGCAGCGT CTTCTTCTCG AATGCACCTT TGAAGCCCTC 
GAAAATGCTG GCATACCAAA GCATACCATT GCCGGAAAGG ACGTTGGCGT TTTTGTCGGC 
GGTTCTTTTC CCGAGTACGA ATCTCATCTC TTCAGAGACC CAGATACCAT ACCTATGCAC 
CAGGCGACCG GTTGTGCATA TGCCATGCAG TCCAACCGCA TATCCCACTT CTTCGACCTA 
CGAGGCCCCA GTTTCACGGC CGATACAGCA TGCTCCTCTA GCATGGTAGC CATACACCTG 
GCTTGCCAGA GCTTGCGAAC CGGTGAATCT AGCGCCGCTT TGGTCGGCGG CTGTCACCTC 
AACATGCTTC CCGAGTTCTG GATCTCCTTT TCGACTTGCA GACTGCTCTC GGATGCCGGC 
CGTTCATTTT CTTTTGACAA TCGCGGCACG GGGTTTGGCC GTGGAGAGGG CTGTGGCATG 
ATTGTCCTGA AGCCACTGGA CCAAGCCATA AAGGACAAGG ATCCCATCAG GGCAGTCATT 
GCCGCAACTG GTCTCAACCA AGATGGCAAG ACGCCCGGCA TCACTGTGCC ACATGGGCCA 
GCGCAAGAGG ACCTCATACG CAAAGTATAT GGCAGGGCTG GCCTCGACCC GAATTTGTGC 
GGCTTCGTCG AGGCCCATGG AACTGGCACT AGGGTTGGTG ATCCCATCGA AGCCACTGCC 
ATTCACAATG TTTTAGGTCA AGAGAGAAGC CCACGCAACC CCCTATGGAT CGGCTCTGTG 
AAATCCAACA TTGGCCATCT GGAAGGGGCC TCCGGTATTG CCGGTGTCAT TAAAGCAGCC 
CTTATGCTTG AGCGGGGCTT CATCCTTCCA AACCATGATT TTAAGCAGCC AAACCCGAGG 
ATCCCCTGGA AAGAATGGAA TTTAGAGGTC CCGGTGACCC AGAGGCCGTG GCCGCGCGGC 
AAAAAGTACA TCAGTGTCAA TAATTTTGGT TTTGGTGGCA CAAATGGCCA TGTTGTACTG 
ACAGCCGCAC CATTCCTGCG TTCCAGAACT TCGGGGTCCT CCCATGATGA CACCCTTATG 
GCTTCGGATA AGTCAGGGCT GGGCCGCAAG CTTTACGTCG TCACGGCTCA TGACAAGGCC 
GCCCTTTCGC AACAGATGAA GAATATCGTG GTCTACCTTG AGCAGAGGCC CGAGATCTTC 
CAAGTAGATC TAACCAGCAA CCTTGCTTAC ACGCTTGGGC AGCGGCGGTC CCTCCTGCAA 
TGGAGAGCTG CCATTCCCGC TATCAACTCA TTTGAGTTAA TCGAAGCTAT CAATGGAGAA 
AAGGCGATAA CCGGTAAAGA AATCGAGCCT CTTAGACTTG GTTTTATTTT TACCGGTCAG 
GGTGCTCAAT GGTATGCCAT GGGTCGGGAA TTGTACGAGC AGTATCCAAC GTTCAGTGCG 
AGTATTTTGC AGGCTGATGC CTGTTTAATC GCTTTCGGGG CGGAATGGTC ACTTGTTGAG 
GAACTCGGAA GAGATGCCAA AACCAGCGGG ATCAGTGAGG CACACATTAG CCAACCATCA 
TGTACTGCAG TACAACTAGC ACTTGTTAAC TTGCTACGAG GCTGGGGCCT GCGGCCAACT 
GCTGTTGCAG GCCACTCGAG TGGTGAGATC GCTGCTGCAT ATGCTGCGGG CATCATTGAA 
TTTGAATCTG CCATGGCAAT TGCCTATCAT CGAGGTAGAC TGATTCCTAT CCTCAAAACG 
AAGTACCCTG AGCTTCAGGG TCGCATGATG GCGGTTGGAG GAAGCAAGAA GGAATTCACA 
ACCCTTATCG AAGGACTACA AGCGAAGGAA GTCCGAATTG CTTGCTACAA TAGTCCTTCC 
AGTTTAACTA TTTCCGGCGA TGAACCTGCC TTGGCCGAAT TGGAGAGGAT TTGCGAGAAG 
AGGCAGCTGT TCAACAGGCG TCTGGTCGTC GACGTCGCTT ACCATTCCCA CCACATGAAC 
TTGGTCGCCA AGGATTACCG TGCTTCCATC GCCAACCTTC AGCCGCCAGC TCGAACTGAC 
GTGCAGTTTC ACTCTTCTCT GTATGGCCAT CCTGTTGACA GTACCGATTT GCAGCCTAAC 
TACTGGGTTG ACAACTTGAC TTGTCCTGTA CGTTTCAATG AGGCGCTGCA GAGCATGCTT 
GCGCCGGTCG GCGAGCATAA GCACGGGGTC AACATGCTTG TCGAGCTCGG CCCCCATTCC 
GTGCTTCAAG GACCAGTCAA GCAGATACTC AAAGAGGTCG GTGGAAGTGC ACCAAAAATT 
CCATATGCTT CTGTTCTTGT TAGAAAACGA AACGCCGTCG AGACCGCTCT GGAACTTGCC 
GCACAGTTGT TCACCAGAGG GGCAAACCTT GATCTTGGTG CCGTCAATTT TCCAAAGACA 
ACCAAACCAC CAACGCTGCT GACAGATTTA CCACGGTATC CCTGGAACCA TTCAATCAGG 
TATTGGCAAG AATCTCGTAT GACAGATGTG CATAAGAACG CGGTTGGTTC AAGGAGCGAC 
ATTATCGGTA CCTTGGCCAG CTACTCGAGC GATTTAGAGC CCACATGGCG CAACATCGTT 
CGTCTGGACG ACCTGCCCTG GCTGCGCCAT CACAAAATTC AATCCTTGAT CATGTATCCC 
ATGTCCGCAT TTCTTGCGAT GGCTGTGGAG GCCGCCTCCC AGAGGGCGAG AAGCAAACAC 
TGCTCATTCG ATAGGTTTCA GATGAGAGAT ATTGCCATAA AAAAGCCCCT AGCGATACCA 
GACAACGACA TCGAGGTTAC AATCAGTCTC CGTCCGCACC AAGAGGGTAT CTTTGTTTCA 
TCCGAGACGT GGGACGAATT CAGGGTTTGC TCATGGTCTT CTGGTCAAGG TTGGATAGAG 
CATTGCGTCG GTTTGATCGC GACGGTGAAA ATTACAGAAA ATGATGTGGT ACAGGGCCCA 
CAGACTGATG ATGGCTTTGA AGACCACCTA CATTCTGTCG TCCCCACCAT CGACAACGCA 
CAATGGGCCT CGATCGATCC CCAGCATATG TACGAGACCC TCAACAAGCT TGGGGTTGTG 
TACGGGCCTA CCTTTCAAGG TCTCGACAAT ATCAACGTGT GCGATACACA GTCAACAGGA 
GATCTTGTTG CTCCAGATGT CGCCAAAGAG ATGCCGGGTG GGTATTTGAC CAAAGCCGTG 
ATACAGCCCG CATTTCTCGA GTCGCTCATT GGCATGTACT GGCCGGTACT TGGAGCCGGA 
CGCCGGCAAG TTGACACGAT TTACCTACCG TCCGCGATCG AGTACGTGAC CATATCTCGA 
AGAATAACCC AACTGGCAAG CGAGCCAGGA AATACGCTGC GCGCCATTTG CAAGGGCGAG 
GTCGATCCTG GCATTCATCG GCCGTTCAAG GTTCACATGG TTGCGGCCCC CGCGGGTTCT 
CTGGAGAGCA TAATTGCAAT CAATGGCTTG ACAGTCTCTC CTGTGCTGAA CGATGATACT 
CGAGAGAAAA ACAACACTCC CCGTGACCTC TGCTACAAAC TTGAATGGGA ACACATCTTG 
GAGCCTGAAC ATGTGAGAAA CCAGCTCCCT GTCGGTGCCC AAATTGTGAT CATTCACGAG 
GATTCAAACT TTCAGTTGCT TGTCGCTCTG AGGTTGGCGA ACGCACTTGA GCAAGCCACG 
GGGAGATTAC CTGAAGTCGG TAAGCTTGAC ACAGTTGATG CAAAAGGAAA GACTTGCATC 
TTCCTAAATG AGCTGCATCG TCCTCTCCTG GCCACCCTTT CGCCATGGCA GTTCTCTAGC 
TTACAAAAGC TTTTGACCGG GGTTGAAGCC ATTCTGTGGG TAGTCAAGGG AGCCTACGAC 
AAGTGCACCG AACCGGAAGC ATATATGGTT ACCGGCCTGA GCAGGTCCAT CCGTTCGGAA 
ACTGCACTTC GGTTTGCCAC ACTGGATCTA GATGCGGCCT CTCCCCTCTC GGAAGCCAAG 
GCCGCAGAGA TGGTTTTTTG CGTCTTCAAC ATGGCTTTTG GGTCTGCTTC TTCGTCGATG 
AGTGAACTGG AGTTTATGGA GAGGGGAGGA TCGCTTTTCA CTCCGCGTAT CACCCATGAC 
AGAGAAACTG ACCAAGAAGT GCAAACCTTG ACAAATCCAA GAGCCTTAGA GCCAACCGCT 
TTTGGGGACG ACGGCCGACT TCTGAGGCTA GAGATATCTG CGCCCGGTGC TTTACATACA 
CTTCACTTCG TCGACGATCA CTCACTGGAA GTGCCACTAG CAATTGATCA ACTTGAGATT 
GAGGTCAAGG CTGTCGGCTT GAGTTTCCGG GACGCCATGG CTGCAAAGGG CCAAATTCCC 
ATCACCATGT CGGGTGCTGA AGCAAGCGGG ATTGTCGTCG CTGTTGGGAG CAGTATCTCC 
GCTTTCAAGG TTGGCGATCG CGTTGCAGCG CTCACGCAAG GCGCTTTTGC GACACGAACG 
AGGGCAACTG CAAGCTTAGC AATCAAGGTC CCAGGACACA TCTCCTTTGA AGCAGCTGCC 
ACACTGCCCC TTGGATACTG CGTTGCATAC TACAGCCTGG TTGATGTCGG GCGCTTGGCT 
GAGGGAGAAA CAGTTCTCAT TCACCAAGCA GCAGGGGCCG TCGGTCAAGC AGCCATTTGC 
CTCGCTCAGA TGATTGGAGC TGAGATTTTC GCAACGGTTG GGAGCGCTGA GAAGAAGGAG 
CTGCTCATTA ATGAGTTCAG TCTTCCTTCC GATCATGTCT TGTACAGTCG CGACACTAGT 
TTTGGGCCTA CTATCCGCCG TGCTACATAT GGCAAGGGGG TAGATGTTAT CCTCAACTCG 
CTGGTTGGCG ATGGCCTACG GGAATCCTGG AAATCTCTCA GCAAGTTTGG ACGGCTCGTG 
GATATAGGCA AGCGAGACTC TGCCTCAAAG TCTCGAACCG AAGTATCCGC TGCCAAGAGA 
AACGCTAGCT TTGTTTCTGT TGACATCTTT GCTCTGTTGG CCGAACGGCC AAAGGCTCTC 
AAACGCGTGT TTGCCGATGT TGCCCGACTT CTCAGATATG GAAAGGTTCG GTCGGCGAGC 
CCGCTATCTG TCTTTCCGGT TTCGGATATT GAGGGCGCAT TGAAGATGCA ACAAAGCGGC 
TCTCCTACTA TGGGCAAGGC AGTCGTGGCT CTAAGTGGGT CTGATTTTGT CAAGGCCCCG 
CCATCACGTC GGTTAAAGGA CCTCCTTCAT TCCGATGCAA CATATATCTT GGTTGGCGGC 
ACCGGTGGTC TCGGAAGGAG TATTGCGCGA TGGATGGCAG GAAAGGGGGC GCAAAACATC 
GTTCTTGTCT CGAGAAGCGC TTCCGTTACC GGCAAGGTCA AGGAACTTGC CGAAGACCTC 
TCTATGATGG GGGTGAGCGT TCATGTCCGC CGCTGCAATG TGGTCAACGA GACTGAAGTG 
GACGAGCTCA TTCGAACTGG GCTACAGGGG CTACCTCCTA TTAGGGGTAT TGTGCTCGGC 
ACGATGGTAT TACACGACGT TCTTTTCGAG AAAATGACAT GGACTGACTA TACCGAAGTC 
ATCAAGGGAA AAGTTCAAGG TGGTTGGAAC TTTCACCGTG CATTGTTGAC CTCGCCACTT 
GATTTCTTCG TGGCCATTTC TTCCGCAGCT AGTGCCGTGG GGAACCGTGG GCAAGCGGCA 
TATAGTGCCG CAAACTGCTT TCTGAATTCT TTGGTCCAGT ACCGGCTTAC CCTTGGTCTT 
CCCGCGTCAT CTCTTGACCT CACCGCTGTT TCCGACTCCG GCTATCTGGC TGAAGACCCG 
GAAAAGGCTG CTGAGGTAGC CCGGAATCTT GGTAGCGATA CGATATGCGA GGCAGAAGTC 
CTCGCCCTGT TGCATGCCGC CATTAGTGGA AAGCTCACGT TGGCCTGCAA CAATCATACG 
ATTACCGGCA TGCGTATTAC CTCGACAATG CGACCGTTCT GGACCGATGA TGCAAAATTC 
AAGACCATGC GCATGGCAGC AGAGGATGAG GCTGCAAGAA ATGCATCATT GAACATGGTT 
GTGTCGTTCA GTGCAACTTT GAAGAATGCC AAATCACTCA TCGAGGCCGA AGACGTCGTC 
TGCAAAGGAC TGGTAGAGAA GATTTCATCT GTGTTAATGC TTGATGCGGA GGATATGGAT 
ATCACACGAT CTCTCTCACA CTATCCCCTT GATTCTTTGG TTGCTATCGA GATCCGCAAC 
TTCATTACTC GTGACTTTGA AGCGAATCTT CAGGTGCTGG AGCTTCTTTC AAGCGGGTCG 
ATTCAAACGT TAGCAAAGGC GGTATGTGCA AAGTCTAAGA TCGTCTCATT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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pks-1 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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