NCU03955 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU03955
Entrez Gene ID	3873684
Full Name	phospholipase D1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_952501.3	XP_957594.3	phospholipase D1

Pathways from BioSystems

Homologies

Neurospora crassa	NCU03955	XP_957594.2
Magnaporthe oryzae (rice blast fungus)	MGG_00960	XP_003717990.1
Schizosaccharomyces pombe (fission yeast)	pld1	NP_592986.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU03955 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU03955 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf10443	XM_952501.3 Latest version!	Neurospora crassa OR74A phospholipase D1 (NCU03955), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU03955 ( XM_952501.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_957594.3 Neurospora crassa OR74A phospholipase D1 (NCU03955), mRNA.

Price & Availability↑

CloneID	ONf10443
Clone ID Related Accession (Same CDS sequence)	XM_952501.3
Accession Version	XM_952501.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	phospholipase D1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506). On Feb 23, 2015 this sequence version replaced XM_952501.2.

ATGGCCGACC CGACCCGATC CAGTTCGGCC AGTCTGCCTG TGCCAACACA GTCGCGCAAA 
GAGAGTGTTC TGCAATCAGG GACCCTCGAG GAACTACCGA CAATCACTCA GGCCCAAGTG 
TTTGGCGTTC CGTCGCCCAT CGGCTCCGAC CAGTTCGACT TCGCCACTAA GGCGAGCTCT 
CCGAAGACAC CGAGAGCTAA CGAAAGCCCG TCCGCCTCGG ACTACCTAAA CGGAGGCCAG 
CCACACAACG ACGGTGTCTC CCCTGTACCC GATCAAGACC CACCCGCCTG GCTGCCGCTC 
CAAGATGTCA ACGAAACCAC TACTCGCTCC TCCGTGCCCT CGACCCTTCC GACAGCTGGC 
ACAGGCCCTT CCGATGTGCC CTACTCCGAG CGCAGCGTAC AGTTTGCCAA CAACGATGTC 
GTTCTCGAAC CCCCACCCGT CATACACCAC GAGCGCCATG CCTCATGGGA CACCAAAGAT 
ACCATGAACA GGATTCGAGG GTCCAGCTTC ATGACCAAGT TGAAGGAGCT CGCCGGCCCC 
AGCGCCTCGC AGACCTTCAA GTCTCCAAGT TACCCTCCCG AAATTAACTC TACAGCTAGC 
TCCCCAACCG TGTCACGCCA TCGTCAAGCC CGGTCAGTCG ACGACCGTAG CGACGAAGAC 
GCCGACGCAG AGGAGACTGC CGATGAGAGG GTGGGTGAGT CACAGATTCG ACAAAAGAAA 
AAGAGACGGA TTCGACGACC AAAGTTCGAA TCGTTCTCGA CCCCCAACAC GCCTCGAATC 
GAGCAAGAGC CGACTACACC CGGTGGCCAT GCCCGCTTCG GCTTTATCCG CCGTTCCACG 
TTCTCAGATA TTGGAGACCG TGGAGGTCTT TCCGAGGGTG AAGGTAGGGA TCATATTGCC 
AGACAGCGCA TCAACAGAAG GACGAATCTG TGGATGTCGA ATCGGCCGGA TGGTGAGGGC 
GACGAACTCG AATCTCCCAG TAGGATCGGT CAGCATCTTC GGCGCTTCTC CAACCTCGGC 
GGCGGGGTTT CTGACGGTGA TGCCACCCCG AGGAGGCCCT TTTTCGGCAC GGACAGGGCT 
GCAACTTTTG GTGCTCAGAA ATGGCGTCAA GTCAAGAGCA CATTGCGGCT GTTGCGACAG 
AAGAGGGACG ACCAATACGA CTATTTCAAA TCTGCCGAGC TCATGGCCGA ACTTACCGCA 
GTTACACCGG CTGTCTTGAT GTTTGCCAGC ATGATCCAGC GCGACGAACA TGGAAACAAA 
AGAATCCCCG TCCTTCTTGA ACAGCTAAGA CTCCGAATCA AGGACAGTGC ACCTCTTCCC 
GGCAAGGATA GCGAGAGACA TGTGCTCTTT ACCATTGAGC TTGAGTACGG AAGCGGTCCC 
AGCCAGATGA AGTGGACGGT CAAACGTCAC ATAAAGGAGA TCATGAACCT CCACTTCAAA 
TACAAGATCA ACCCCCCTAA AGACAAGCTG TTGATACATC GGCCAGAGGG CGGTGTTCGG 
CCCAAACAGC CAAATTTCCC CATCTCGGCT TTCCCTTATT TGCGCGCAGT AAGAGGCTTG 
GAGGACGACG AGGAACAAGA ATCACTTGAC CCCCCTCGTA TAGTCGAAGA TCCCGCCGGC 
GAGGCCACGG CCGGGGAAAC AGCTGTTGAG GGCACTGATG TTGATGAAAA TGGCAGGCCG 
CAGGTACGGA AAAAGAAGTC TCGCATGGGT GCTTTTGGTG GGCGCCGCAA GAACTCGGCC 
ATAGGCTCAA AGGGTGATCT GAATCAAATA GCTCTTGACG CCGCCGCCAA GAAGAAAAAG 
TATATCGAAC AGCAGCAGAG AATCCTGGAA AAGTATCTGC AAGAGATGGT TCGTTGGCTC 
ATGTTCCGGG CAGACAGTAA CCGACTTTGT CGATTTTTGG AGCTTTCTGC TCTTGGAGTT 
CGCCTTGCTG CCGAGGGAAG TTATCACGGC AAGGAATGTT ACCTCCACAT CCAGTCCTCC 
AAAGGGCTCG ATTTCCGGCG GGTCCTGACA CCGGGCAAGG TTATTGCGAG GCACAGCAAG 
AAGTGGTTCC TTGTTCGTCA GAGTTATATC GTCTGTGTCG AATCGCCGGA AAACATGAAC 
ATCTACGACG TTTATCTTGT TGACCCCAAA TTCAACATTG TTTCTAAGAA GGACAAGAAC 
AAGCACCTCA CAGCAAACGG CAATGAAAAT GAGGAAGACC TCGAAAATTT CGACCCGACC 
ACGGCCAAGA AGTCCAAGTC CTCCAACCAC CATACACTCA AGATCGTGTC ATCCGAGAGG 
AAGATCAAGC TCTTCTCCTC CAGCCAGCAT CTGATACAGC AGTTCGAGGA ATCCATTTTG 
GAGATGCTCA AAACTACACC ATGGCATCAG CGGAATCGCT TTGGAAGCTA TGCGCCTGTT 
CGAACTGGCG TGTTCGCCCA ATGGCTGGTC GACGGTCGGG ACTACATGTG GAACGTCTCA 
AGAGCCATCA GTATGGCCAA GGACGTTATC TACATTCACG ACTGGTGGCT GAGCCCAGAA 
TTGTACATGA GGCGGCCCGC TTGTATCAGT CAAAAATGGC GTCTGGATCG TCTACTCCAG 
AGAAAGGCAC AGGAAGGTGT CAAGATTTTT GTCATTGTCT ATCGAAATGT CGAAGCCGCA 
GTTCCCATTG ACTCGGAGTA CACCAAGTTT TCCCTGCTGA ACCTCCATCC TAACATTTTC 
GTTCAAAGAT CACCTAACCA GTTCAAGAAG AACCAGTTCT TCTTCGCTCA CCATGAAAAG 
CTGGTCATAG TCGACCACGA TATTGCATTT GTGGGCGGTA TCGATCTGTG TTTCGGCCGA 
TGGGACACGC CCCAGCATCC AGTGACCGAT GACAAACCCA CTGGATTCGA GCCTGATTCG 
TACAATTTAC CCAAGGATGC AGAGCATTGT CAAATGTTCC CGGGAAAGGA TTACTCGAAT 
CCTCGTGTCC AGGACTTTGT CAGGCTCCAT GAGCCATATG AGGAGATGTA TGACCGGTCC 
AAGGTTCCCA GAATGCCGTG GCATGATATC GCCATGCAGG TTGTTGGCCA GCCAGCCAGA 
GATCTTACCC GCCACTTCGT TCAGAGATGG AATTATGTGA GACGTGGCCG TAAGCCAACA 
CGGCCTACAC CATTCTTGCT GCCACCTCCT GACTGCAGCA GAGAGGAACT CGAGGCCGCA 
GGCCTCAATG GAACTTGCGA GGTACAAATG CTCCGTTCAG CATCCACGTG GTCGATCGGT 
ATCGATGAGA CCGAACACAG CATCCAGTCA GCATATGTCA AGATGATCGA AGAGTCTGAT 
CACTTTGTGT ACATGGAGAA CCAGTTCTTC GTGACCAGCA CAGAAACATT GAACGTGAAA 
ATCGTCAATC ATATCGGCGA TGCTCTGGTT GAGCGCGCTA TCCGTGCGCA CGAAAAGGGC 
GAGGACTGGC GCGCAGTCAT TATTATTCCC CTGATGCCAG GTTTCCAAAA CGAGGTCAAT 
GACCAGGACG GAACCAGTGT TCGCTTGATT CTCCAATGTC AGTATCGGAG TATCTGCCGC 
GGAGAACACT CCATCTTTGG CCGTCTTAGG GCTGCCGGCA TCAACCCGGA GGACTACATC 
CAGTTCTTTA GTCTGCGTCA ATGGGGTAAA CTCAAGAACA ACTCTCTGAC GACCGAACAA 
CTCTATATCC ACGCCAAGTG TATCATTGTC GATGACCGTA TTGCCTTGAT CGGATCAGCC 
AACATCAATG AGCGGTCTAT GCTTGGCAAT CGTGATTCGG AATGCGCGGC CGTTGTTCGT 
GACACTGACA TGATCTGGTC TACCATGGGT GGGAAGCCTT ACCAGGTTGG TCGTTTTGCT 
CACACCCTGC GTCTCCGACT GATGAGGGAA CACTTGGGCT TGGATGTCGA TGAGATCCAA 
GAGGAAGAAC GTCAAGCAGA GCTTGACGAG GCTGAGGCAA GAGCTAAGAT GGAACAACGC 
ACGGAACACC ACATGGAGCA CCCGAACTTG GAACATCGCG ACTTCACTGA CGACTCGGAA 
GATTCCATGG TCCCAGAAAG CTCGTCCAGG AGACGGAGGT CATCTCATAT GTCGTCTCAT 
ATTCTACCTA GCACGCCGCC GCCGCCGCAA CTCCCTCGTC ATAGAGCGCA TAGTTTCAAT 
CATGACGTTG AAATGCAGGA ACTCCCAAGG CCAGACTCTA AGGGCAAATC CGTTGCCGGC 
AGAGAAGACC AGGAGCATGT GCATGCCGGT GAAAGGCACA GGAGGGATGT GGAAGGATTG 
GGTCCTGATC ATTGGAAGCT TGCTCAAATG CAAGGTATTG ATGAGGGCAG AGACTCTGTC 
GTGGTGAATG GCCGTGAAGT GCTGGTTCAC GACATCTCTG CGGAGGGCAA GGGTACGCTG 
GACAGCCCGC ATGAGTCTCA TGAGCTTCGC ACCCGGCCTC ATGATGCTGC GTCTCTTTCT 
GAAAGCATCG GCAATGAGCA CATGCCGCCA ATGCCACCGT TCAACAGACG GACTACGGAG 
CAGCTTGGTC TTCCCAGAGC CAATCAGTTG CCTGTCCTGC CTGTTGTTGA CGATACTGAT 
ATTGGTGGAC CGCCTGTTCA GATTACAGCA AACGGGACAC TGTCTCACAG CCAGATCGCC 
ATGAACATTG AGCTGGCCAA CATTGACAAA GACTGCATGC GTGATCCTCT TAATCCAAGT 
TTCTATGAGG ATGTTTGGTC TCGTGTTGCT GAGAACAACA CCAAGATCTA CCGCCGCGTC 
TTCCATGTCA TGCCCGATTC TGCAGTAACG AACTGGGCCG AGTACAAGGA GTTCAAGGCT 
AACGAGCGGG AGTTCAAGAA GCAGTTTGAA GGTGGCAAGG CAAGCGAGGA AGTCAATGAG 
AAGAACGGAG TGACCGCTGA GCATGCGCAA ACCAGTGCTG GAGCCGGTAT CAGCGCACCG 
GGCCCAGCTC AACTCACCAA GAGCTTGACG GAGAAGCTAG TCACTCCTGT CTCCAAGCTC 
AGCGGCGGTG GTGACAAGGG AGCTCGGGAA CGCACAGGCT CAACTACTAC GTTGGACGAG 
AAGGGGGCCA CGAAGGACCA CACACGCAAG GCTCAGACCT TCCCACTCGA TGCTGAGAAG 
GGTGCGATGT TGACGGCGTC GCAGAACCCC GGCGAACGCC CACCCACAGC AACACCCAAA 
GACAGCGCTG ATTCGAACCA CGATGATACC AGGGCGTCTG GAGACTTCCT GAGCGCCAGC 
ATCACCAACA ACAAGGAGCG ACACGCCACC TTCTCCAACA ACCAAGATCC TAACCCTCCC 
CCAACCTCAG CCAGCGGCGG CTCCACCACC ACCATAGCCG ACAACAGCAA CGCAGCCCCC 
AACATTGCCA ACGGCAACGG CGCCGCAGTA TCCTCCGACA ACACAGCAAC CGCATCCGCC 
ACGGGCACTA ATGGTGCTAA TGTCAAACCA GCCGCATCCA CCAGGCAGCA GCGTCGTAGG 
GCGAACACTG GCCGTAGTAA GCGCGGGTAT AATCCATTCG ACGCTCAGAC TCTAAACCCG 
GCAGAGGCCG AGGAGACGCT CAGGTTGACC CAGGGAACGC TGGTCCAGTT TCCTTATGAC 
TGGCTGGTCA CGGAGGAGAA CAACGGGAAT TGGTTGTTCC AGGTTGATCA AGTGGCACCG 
TTGCAGATTT ATAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_957594.3
CDS	525..6182
Translation	MADPTRSSSASLPVPTQSRKESVLQSGTLEELPTITQAQVFGVPSPIGSDQFDFATKASSPKTPRANESPSASDYLNGGQPHNDGVSPVPDQDPPAWLPLQDVNETTTRSSVPSTLPTAGTGPSDVPYSERSVQFANNDVVLEPPPVIHHERHASWDTKDTMNRIRGSSFMTKLKELAGPSASQTFKSPSYPPEINSTASSPTVSRHRQARSVDDRSDEDADAEETADERVGESQIRQKKKRRIRRPKFESFSTPNTPRIEQEPTTPGGHARFGFIRRSTFSDIGDRGGLSEGEGRDHIARQRINRRTNLWMSNRPDGEGDELESPSRIGQHLRRFSNLGGGVSDGDATPRRPFFGTDRAATFGAQKWRQVKSTLRLLRQKRDDQYDYFKSAELMAELTAVTPAVLMFASMIQRDEHGNKRIPVLLEQLRLRIKDSAPLPGKDSERHVLFTIELEYGSGPSQMKWTVKRHIKEIMNLHFKYKINPPKDKLLIHRPEGGVRPKQPNFPISAFPYLRAVRGLEDDEEQESLDPPRIVEDPAGEATAGETAVEGTDVDENGRPQVRKKKSRMGAFGGRRKNSAIGSKGDLNQIALDAAAKKKKYIEQQQRILEKYLQEMVRWLMFRADSNRLCRFLELSALGVRLAAEGSYHGKECYLHIQSSKGLDFRRVLTPGKVIARHSKKWFLVRQSYIVCVESPENMNIYDVYLVDPKFNIVSKKDKNKHLTANGNENEEDLENFDPTTAKKSKSSNHHTLKIVSSERKIKLFSSSQHLIQQFEESILEMLKTTPWHQRNRFGSYAPVRTGVFAQWLVDGRDYMWNVSRAISMAKDVIYIHDWWLSPELYMRRPACISQKWRLDRLLQRKAQEGVKIFVIVYRNVEAAVPIDSEYTKFSLLNLHPNIFVQRSPNQFKKNQFFFAHHEKLVIVDHDIAFVGGIDLCFGRWDTPQHPVTDDKPTGFEPDSYNLPKDAEHCQMFPGKDYSNPRVQDFVRLHEPYEEMYDRSKVPRMPWHDIAMQVVGQPARDLTRHFVQRWNYVRRGRKPTRPTPFLLPPPDCSREELEAAGLNGTCEVQMLRSASTWSIGIDETEHSIQSAYVKMIEESDHFVYMENQFFVTSTETLNVKIVNHIGDALVERAIRAHEKGEDWRAVIIIPLMPGFQNEVNDQDGTSVRLILQCQYRSICRGEHSIFGRLRAAGINPEDYIQFFSLRQWGKLKNNSLTTEQLYIHAKCIIVDDRIALIGSANINERSMLGNRDSECAAVVRDTDMIWSTMGGKPYQVGRFAHTLRLRLMREHLGLDVDEIQEEERQAELDEAEARAKMEQRTEHHMEHPNLEHRDFTDDSEDSMVPESSSRRRRSSHMSSHILPSTPPPPQLPRHRAHSFNHDVEMQELPRPDSKGKSVAGREDQEHVHAGERHRRDVEGLGPDHWKLAQMQGIDEGRDSVVVNGREVLVHDISAEGKGTLDSPHESHELRTRPHDAASLSESIGNEHMPPMPPFNRRTTEQLGLPRANQLPVLPVVDDTDIGGPPVQITANGTLSHSQIAMNIELANIDKDCMRDPLNPSFYEDVWSRVAENNTKIYRRVFHVMPDSAVTNWAEYKEFKANEREFKKQFEGGKASEEVNEKNGVTAEHAQTSAGAGISAPGPAQLTKSLTEKLVTPVSKLSGGGDKGARERTGSTTTLDEKGATKDHTRKAQTFPLDAEKGAMLTASQNPGERPPTATPKDSADSNHDDTRASGDFLSASITNNKERHATFSNNQDPNPPPTSASGGSTTTIADNSNAAPNIANGNGAAVSSDNTATASATGTNGANVKPAASTRQQRRRANTGRSKRGYNPFDAQTLNPAEAEETLRLTQGTLVQFPYDWLVTEENNGNWLFQVDQVAPLQIYN

Target ORF information:

RefSeq Version	XM_952501.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A phospholipase D1 (NCU03955), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_952501.3

ORF Insert Sequence:

ATGGCCGACC CGACCCGATC CAGTTCGGCC AGTCTGCCTG TGCCAACACA GTCGCGCAAA 
GAGAGTGTTC TGCAATCAGG GACCCTCGAG GAACTACCGA CAATCACTCA GGCCCAAGTG 
TTTGGCGTTC CGTCGCCCAT CGGCTCCGAC CAGTTCGACT TCGCCACTAA GGCGAGCTCT 
CCGAAGACAC CGAGAGCTAA CGAAAGCCCG TCCGCCTCGG ACTACCTAAA CGGAGGCCAG 
CCACACAACG ACGGTGTCTC CCCTGTACCC GATCAAGACC CACCCGCCTG GCTGCCGCTC 
CAAGATGTCA ACGAAACCAC TACTCGCTCC TCCGTGCCCT CGACCCTTCC GACAGCTGGC 
ACAGGCCCTT CCGATGTGCC CTACTCCGAG CGCAGCGTAC AGTTTGCCAA CAACGATGTC 
GTTCTCGAAC CCCCACCCGT CATACACCAC GAGCGCCATG CCTCATGGGA CACCAAAGAT 
ACCATGAACA GGATTCGAGG GTCCAGCTTC ATGACCAAGT TGAAGGAGCT CGCCGGCCCC 
AGCGCCTCGC AGACCTTCAA GTCTCCAAGT TACCCTCCCG AAATTAACTC TACAGCTAGC 
TCCCCAACCG TGTCACGCCA TCGTCAAGCC CGGTCAGTCG ACGACCGTAG CGACGAAGAC 
GCCGACGCAG AGGAGACTGC CGATGAGAGG GTGGGTGAGT CACAGATTCG ACAAAAGAAA 
AAGAGACGGA TTCGACGACC AAAGTTCGAA TCGTTCTCGA CCCCCAACAC GCCTCGAATC 
GAGCAAGAGC CGACTACACC CGGTGGCCAT GCCCGCTTCG GCTTTATCCG CCGTTCCACG 
TTCTCAGATA TTGGAGACCG TGGAGGTCTT TCCGAGGGTG AAGGTAGGGA TCATATTGCC 
AGACAGCGCA TCAACAGAAG GACGAATCTG TGGATGTCGA ATCGGCCGGA TGGTGAGGGC 
GACGAACTCG AATCTCCCAG TAGGATCGGT CAGCATCTTC GGCGCTTCTC CAACCTCGGC 
GGCGGGGTTT CTGACGGTGA TGCCACCCCG AGGAGGCCCT TTTTCGGCAC GGACAGGGCT 
GCAACTTTTG GTGCTCAGAA ATGGCGTCAA GTCAAGAGCA CATTGCGGCT GTTGCGACAG 
AAGAGGGACG ACCAATACGA CTATTTCAAA TCTGCCGAGC TCATGGCCGA ACTTACCGCA 
GTTACACCGG CTGTCTTGAT GTTTGCCAGC ATGATCCAGC GCGACGAACA TGGAAACAAA 
AGAATCCCCG TCCTTCTTGA ACAGCTAAGA CTCCGAATCA AGGACAGTGC ACCTCTTCCC 
GGCAAGGATA GCGAGAGACA TGTGCTCTTT ACCATTGAGC TTGAGTACGG AAGCGGTCCC 
AGCCAGATGA AGTGGACGGT CAAACGTCAC ATAAAGGAGA TCATGAACCT CCACTTCAAA 
TACAAGATCA ACCCCCCTAA AGACAAGCTG TTGATACATC GGCCAGAGGG CGGTGTTCGG 
CCCAAACAGC CAAATTTCCC CATCTCGGCT TTCCCTTATT TGCGCGCAGT AAGAGGCTTG 
GAGGACGACG AGGAACAAGA ATCACTTGAC CCCCCTCGTA TAGTCGAAGA TCCCGCCGGC 
GAGGCCACGG CCGGGGAAAC AGCTGTTGAG GGCACTGATG TTGATGAAAA TGGCAGGCCG 
CAGGTACGGA AAAAGAAGTC TCGCATGGGT GCTTTTGGTG GGCGCCGCAA GAACTCGGCC 
ATAGGCTCAA AGGGTGATCT GAATCAAATA GCTCTTGACG CCGCCGCCAA GAAGAAAAAG 
TATATCGAAC AGCAGCAGAG AATCCTGGAA AAGTATCTGC AAGAGATGGT TCGTTGGCTC 
ATGTTCCGGG CAGACAGTAA CCGACTTTGT CGATTTTTGG AGCTTTCTGC TCTTGGAGTT 
CGCCTTGCTG CCGAGGGAAG TTATCACGGC AAGGAATGTT ACCTCCACAT CCAGTCCTCC 
AAAGGGCTCG ATTTCCGGCG GGTCCTGACA CCGGGCAAGG TTATTGCGAG GCACAGCAAG 
AAGTGGTTCC TTGTTCGTCA GAGTTATATC GTCTGTGTCG AATCGCCGGA AAACATGAAC 
ATCTACGACG TTTATCTTGT TGACCCCAAA TTCAACATTG TTTCTAAGAA GGACAAGAAC 
AAGCACCTCA CAGCAAACGG CAATGAAAAT GAGGAAGACC TCGAAAATTT CGACCCGACC 
ACGGCCAAGA AGTCCAAGTC CTCCAACCAC CATACACTCA AGATCGTGTC ATCCGAGAGG 
AAGATCAAGC TCTTCTCCTC CAGCCAGCAT CTGATACAGC AGTTCGAGGA ATCCATTTTG 
GAGATGCTCA AAACTACACC ATGGCATCAG CGGAATCGCT TTGGAAGCTA TGCGCCTGTT 
CGAACTGGCG TGTTCGCCCA ATGGCTGGTC GACGGTCGGG ACTACATGTG GAACGTCTCA 
AGAGCCATCA GTATGGCCAA GGACGTTATC TACATTCACG ACTGGTGGCT GAGCCCAGAA 
TTGTACATGA GGCGGCCCGC TTGTATCAGT CAAAAATGGC GTCTGGATCG TCTACTCCAG 
AGAAAGGCAC AGGAAGGTGT CAAGATTTTT GTCATTGTCT ATCGAAATGT CGAAGCCGCA 
GTTCCCATTG ACTCGGAGTA CACCAAGTTT TCCCTGCTGA ACCTCCATCC TAACATTTTC 
GTTCAAAGAT CACCTAACCA GTTCAAGAAG AACCAGTTCT TCTTCGCTCA CCATGAAAAG 
CTGGTCATAG TCGACCACGA TATTGCATTT GTGGGCGGTA TCGATCTGTG TTTCGGCCGA 
TGGGACACGC CCCAGCATCC AGTGACCGAT GACAAACCCA CTGGATTCGA GCCTGATTCG 
TACAATTTAC CCAAGGATGC AGAGCATTGT CAAATGTTCC CGGGAAAGGA TTACTCGAAT 
CCTCGTGTCC AGGACTTTGT CAGGCTCCAT GAGCCATATG AGGAGATGTA TGACCGGTCC 
AAGGTTCCCA GAATGCCGTG GCATGATATC GCCATGCAGG TTGTTGGCCA GCCAGCCAGA 
GATCTTACCC GCCACTTCGT TCAGAGATGG AATTATGTGA GACGTGGCCG TAAGCCAACA 
CGGCCTACAC CATTCTTGCT GCCACCTCCT GACTGCAGCA GAGAGGAACT CGAGGCCGCA 
GGCCTCAATG GAACTTGCGA GGTACAAATG CTCCGTTCAG CATCCACGTG GTCGATCGGT 
ATCGATGAGA CCGAACACAG CATCCAGTCA GCATATGTCA AGATGATCGA AGAGTCTGAT 
CACTTTGTGT ACATGGAGAA CCAGTTCTTC GTGACCAGCA CAGAAACATT GAACGTGAAA 
ATCGTCAATC ATATCGGCGA TGCTCTGGTT GAGCGCGCTA TCCGTGCGCA CGAAAAGGGC 
GAGGACTGGC GCGCAGTCAT TATTATTCCC CTGATGCCAG GTTTCCAAAA CGAGGTCAAT 
GACCAGGACG GAACCAGTGT TCGCTTGATT CTCCAATGTC AGTATCGGAG TATCTGCCGC 
GGAGAACACT CCATCTTTGG CCGTCTTAGG GCTGCCGGCA TCAACCCGGA GGACTACATC 
CAGTTCTTTA GTCTGCGTCA ATGGGGTAAA CTCAAGAACA ACTCTCTGAC GACCGAACAA 
CTCTATATCC ACGCCAAGTG TATCATTGTC GATGACCGTA TTGCCTTGAT CGGATCAGCC 
AACATCAATG AGCGGTCTAT GCTTGGCAAT CGTGATTCGG AATGCGCGGC CGTTGTTCGT 
GACACTGACA TGATCTGGTC TACCATGGGT GGGAAGCCTT ACCAGGTTGG TCGTTTTGCT 
CACACCCTGC GTCTCCGACT GATGAGGGAA CACTTGGGCT TGGATGTCGA TGAGATCCAA 
GAGGAAGAAC GTCAAGCAGA GCTTGACGAG GCTGAGGCAA GAGCTAAGAT GGAACAACGC 
ACGGAACACC ACATGGAGCA CCCGAACTTG GAACATCGCG ACTTCACTGA CGACTCGGAA 
GATTCCATGG TCCCAGAAAG CTCGTCCAGG AGACGGAGGT CATCTCATAT GTCGTCTCAT 
ATTCTACCTA GCACGCCGCC GCCGCCGCAA CTCCCTCGTC ATAGAGCGCA TAGTTTCAAT 
CATGACGTTG AAATGCAGGA ACTCCCAAGG CCAGACTCTA AGGGCAAATC CGTTGCCGGC 
AGAGAAGACC AGGAGCATGT GCATGCCGGT GAAAGGCACA GGAGGGATGT GGAAGGATTG 
GGTCCTGATC ATTGGAAGCT TGCTCAAATG CAAGGTATTG ATGAGGGCAG AGACTCTGTC 
GTGGTGAATG GCCGTGAAGT GCTGGTTCAC GACATCTCTG CGGAGGGCAA GGGTACGCTG 
GACAGCCCGC ATGAGTCTCA TGAGCTTCGC ACCCGGCCTC ATGATGCTGC GTCTCTTTCT 
GAAAGCATCG GCAATGAGCA CATGCCGCCA ATGCCACCGT TCAACAGACG GACTACGGAG 
CAGCTTGGTC TTCCCAGAGC CAATCAGTTG CCTGTCCTGC CTGTTGTTGA CGATACTGAT 
ATTGGTGGAC CGCCTGTTCA GATTACAGCA AACGGGACAC TGTCTCACAG CCAGATCGCC 
ATGAACATTG AGCTGGCCAA CATTGACAAA GACTGCATGC GTGATCCTCT TAATCCAAGT 
TTCTATGAGG ATGTTTGGTC TCGTGTTGCT GAGAACAACA CCAAGATCTA CCGCCGCGTC 
TTCCATGTCA TGCCCGATTC TGCAGTAACG AACTGGGCCG AGTACAAGGA GTTCAAGGCT 
AACGAGCGGG AGTTCAAGAA GCAGTTTGAA GGTGGCAAGG CAAGCGAGGA AGTCAATGAG 
AAGAACGGAG TGACCGCTGA GCATGCGCAA ACCAGTGCTG GAGCCGGTAT CAGCGCACCG 
GGCCCAGCTC AACTCACCAA GAGCTTGACG GAGAAGCTAG TCACTCCTGT CTCCAAGCTC 
AGCGGCGGTG GTGACAAGGG AGCTCGGGAA CGCACAGGCT CAACTACTAC GTTGGACGAG 
AAGGGGGCCA CGAAGGACCA CACACGCAAG GCTCAGACCT TCCCACTCGA TGCTGAGAAG 
GGTGCGATGT TGACGGCGTC GCAGAACCCC GGCGAACGCC CACCCACAGC AACACCCAAA 
GACAGCGCTG ATTCGAACCA CGATGATACC AGGGCGTCTG GAGACTTCCT GAGCGCCAGC 
ATCACCAACA ACAAGGAGCG ACACGCCACC TTCTCCAACA ACCAAGATCC TAACCCTCCC 
CCAACCTCAG CCAGCGGCGG CTCCACCACC ACCATAGCCG ACAACAGCAA CGCAGCCCCC 
AACATTGCCA ACGGCAACGG CGCCGCAGTA TCCTCCGACA ACACAGCAAC CGCATCCGCC 
ACGGGCACTA ATGGTGCTAA TGTCAAACCA GCCGCATCCA CCAGGCAGCA GCGTCGTAGG 
GCGAACACTG GCCGTAGTAA GCGCGGGTAT AATCCATTCG ACGCTCAGAC TCTAAACCCG 
GCAGAGGCCG AGGAGACGCT CAGGTTGACC CAGGGAACGC TGGTCCAGTT TCCTTATGAC 
TGGCTGGTCA CGGAGGAGAA CAACGGGAAT TGGTTGTTCC AGGTTGATCA AGTGGCACCG 
TTGCAGATTT ATAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU03955 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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