NCU02481 cDNA ORF clone, Neurospora crassa OR74A

The following NCU02481 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU02481 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf08357 XM_959982.2
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Neurospora crassa OR74A mitochondrial 2-methylisocitrate lyase (NCU02481), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf08357
    Clone ID Related Accession (Same CDS sequence) XM_959982.2
    Accession Version XM_959982.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1857bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product mitochondrial 2-methylisocitrate lyase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_959982.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCTTCGCA GGGCAATCCC CAGAATCTCC CGGCGAACTG TCCCGGCGCA GACTTCATCC 
    CCCCTCTTCG GTCTTTGCGC CCGCCCCGTG AGAACCAGCA CTACCACCTC CGCGCACGCT
    CGGCTCCTCT CGACATCAGC TGCTAGGATG ACCGCTCCCT CGCCTCTGGC TCCTGTTGAG
    CATCCTTTCT CCTCGACCAT TCCTTTTGAG TCCTTCCAGC TTTTGCCCGA GGAGCAGAAA
    GCCGGCGCGG CCGAAGATGC CCTCCTCCAA CAACAGATCA AGGAAGTCGA GGCTTGGTGG
    GCGTCGCCGC GCTTCAAGGG CATCAAGCGC GACTGGACTG CTGCCGATGT CGTGAGCAAG
    CGCGGTTCGC TCCAGCAGAC CTACACTTCG TCCGTCATGG CGCGCAAGCT CTGGAACTTG
    GTGCAAGAGC GCTACGCCCA GGGCAAGCCC ATCCATACTC TGGGTGCCAT CGATCCCGTT
    CAGATGACCC AGCAGGCGCC TCATCAGGAG GTCCTCTACA TTTCTGGTTG GGCATGCTCC
    TCGGTTCTGA CTTCCACCAA CGAGGTATCG CCCGACTTTG GCGACTACCC CTACAACACT
    GTTCCCAACC AGGTCCAGCG TCTCCACAAG GCCCAGAGCA TGCATGACCG CAAGCAATGG
    TTCCTGCGCA GCAAGATGAC TCCTGAGGAG CGGGCCAAGA CCCCTTATAC CGACTACTTC
    CGCCCCATCA TTGCCGACGG CGACACCGGC CACGGCGGCT TGACTGCCGT CATGAAGCTT
    GCCAAGCTGT TCGCCGAGAA TGGTGCCGCC GGCGTTCACT TTGAGGATCA GATGCACGGT
    GGCAAGAAGT GCGGTCACCT TGCCGGCAAG GTTCTCGTCC CCACCGGCGA GCACATCAAC
    CGCCTCAAGG CCGCCCGCTT CCAGTGGGAC GTCATGGGCA CCGAGAACCT TGTGATCGCT
    CGCACGGACT CCGAGAGTGG TAAGCTGCTT TCTTCCTCCA TCGACGCCCG CGATCACGAG
    TTCATCCTCG GTGTCGCCGA CCCCTCCATC ACCTCCCTCG CCGAGACCCT CGCCGACATG
    GAAGCCCGTG GTGCTCCCGG TTCCGAGATC GACGCCTATG AGGCCGAGTG GGTCAAGAAC
    ACGAAGCTCG TCACCTTCGA CGAGGCTGCT CTTGCCCACT TTGAGCACCA CAACGTCCGC
    CAGGACCTCT TCGCCACCTA CAAGGCCACC ATTGCCCAGA ACCCCAACAT GGGCATCACG
    GCCCGCCGTG CCCTGGCCCA ATCCATCACC CCCGAACACC CGGTGTACTG GGACTGGGAC
    GTGCCGCGCA CGCGCGAGGG CTTCTACCAC TTCCGTAACG GCATGGCGGC CGCCACCAAG
    CGCGCCCTCG CCTTCGGTCC CTACGCCGAC CTCCTCTGGG TCGAGACGGG CGACCCGTCC
    GTCGAGGTGG CCACGGAGCT CGGCCGCGCC GTGCGCGAAC AGTTCCCCGG CAAGGGTCTC
    GTATACAACC TTTCCCCTTC GTTCAACTGG ATGGCCCATG GCTTCACCCA GGACACGCTC
    AAGTCGTTCG TCTGGGACAT TGCGAAGGAA GGTTTCACCC TTCAGCTCGT CTCGCTCGCG
    GGTCTGCACA GCACCGCCAC CATCAGCAAC GAGCTGGCCC GCCGCTACAA GAACGAGGGC
    ATGCAGGCGT ATGTTGAGCT GGTGCAGAAG CGCGAGAAGG AGCTCGGTGT TGATGTGCTC
    ACGCACCAGA AGTGGAGCGG CGCCAACTAT GTTGATGCCA TTCTGGGACA CATTCAGAGC
    GGTAGCTCGA GTAGCAAGAG CATGGGCGAG GGTAACACTG AGAACCAGTT CGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_965075.1
    CDS545..2401
    Translation

    Target ORF information:

    RefSeq Version XM_959982.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A mitochondrial 2-methylisocitrate lyase (NCU02481), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_959982.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCTTCGCA GGGCAATCCC CAGAATCTCC CGGCGAACTG TCCCGGCGCA GACTTCATCC 
    CCCCTCTTCG GTCTTTGCGC CCGCCCCGTG AGAACCAGCA CTACCACCTC CGCGCACGCT
    CGGCTCCTCT CGACATCAGC TGCTAGGATG ACCGCTCCCT CGCCTCTGGC TCCTGTTGAG
    CATCCTTTCT CCTCGACCAT TCCTTTTGAG TCCTTCCAGC TTTTGCCCGA GGAGCAGAAA
    GCCGGCGCGG CCGAAGATGC CCTCCTCCAA CAACAGATCA AGGAAGTCGA GGCTTGGTGG
    GCGTCGCCGC GCTTCAAGGG CATCAAGCGC GACTGGACTG CTGCCGATGT CGTGAGCAAG
    CGCGGTTCGC TCCAGCAGAC CTACACTTCG TCCGTCATGG CGCGCAAGCT CTGGAACTTG
    GTGCAAGAGC GCTACGCCCA GGGCAAGCCC ATCCATACTC TGGGTGCCAT CGATCCCGTT
    CAGATGACCC AGCAGGCGCC TCATCAGGAG GTCCTCTACA TTTCTGGTTG GGCATGCTCC
    TCGGTTCTGA CTTCCACCAA CGAGGTATCG CCCGACTTTG GCGACTACCC CTACAACACT
    GTTCCCAACC AGGTCCAGCG TCTCCACAAG GCCCAGAGCA TGCATGACCG CAAGCAATGG
    TTCCTGCGCA GCAAGATGAC TCCTGAGGAG CGGGCCAAGA CCCCTTATAC CGACTACTTC
    CGCCCCATCA TTGCCGACGG CGACACCGGC CACGGCGGCT TGACTGCCGT CATGAAGCTT
    GCCAAGCTGT TCGCCGAGAA TGGTGCCGCC GGCGTTCACT TTGAGGATCA GATGCACGGT
    GGCAAGAAGT GCGGTCACCT TGCCGGCAAG GTTCTCGTCC CCACCGGCGA GCACATCAAC
    CGCCTCAAGG CCGCCCGCTT CCAGTGGGAC GTCATGGGCA CCGAGAACCT TGTGATCGCT
    CGCACGGACT CCGAGAGTGG TAAGCTGCTT TCTTCCTCCA TCGACGCCCG CGATCACGAG
    TTCATCCTCG GTGTCGCCGA CCCCTCCATC ACCTCCCTCG CCGAGACCCT CGCCGACATG
    GAAGCCCGTG GTGCTCCCGG TTCCGAGATC GACGCCTATG AGGCCGAGTG GGTCAAGAAC
    ACGAAGCTCG TCACCTTCGA CGAGGCTGCT CTTGCCCACT TTGAGCACCA CAACGTCCGC
    CAGGACCTCT TCGCCACCTA CAAGGCCACC ATTGCCCAGA ACCCCAACAT GGGCATCACG
    GCCCGCCGTG CCCTGGCCCA ATCCATCACC CCCGAACACC CGGTGTACTG GGACTGGGAC
    GTGCCGCGCA CGCGCGAGGG CTTCTACCAC TTCCGTAACG GCATGGCGGC CGCCACCAAG
    CGCGCCCTCG CCTTCGGTCC CTACGCCGAC CTCCTCTGGG TCGAGACGGG CGACCCGTCC
    GTCGAGGTGG CCACGGAGCT CGGCCGCGCC GTGCGCGAAC AGTTCCCCGG CAAGGGTCTC
    GTATACAACC TTTCCCCTTC GTTCAACTGG ATGGCCCATG GCTTCACCCA GGACACGCTC
    AAGTCGTTCG TCTGGGACAT TGCGAAGGAA GGTTTCACCC TTCAGCTCGT CTCGCTCGCG
    GGTCTGCACA GCACCGCCAC CATCAGCAAC GAGCTGGCCC GCCGCTACAA GAACGAGGGC
    ATGCAGGCGT ATGTTGAGCT GGTGCAGAAG CGCGAGAAGG AGCTCGGTGT TGATGTGCTC
    ACGCACCAGA AGTGGAGCGG CGCCAACTAT GTTGATGCCA TTCTGGGACA CATTCAGAGC
    GGTAGCTCGA GTAGCAAGAG CATGGGCGAG GGTAACACTG AGAACCAGTT CGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B