Phrf1 cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Phrf1
Entrez Gene ID	103751079
Full Name	PHD and ring finger domains 1
Gene Type	protein-coding
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029556781.1	XP_029412641.1	PHD and RING finger domain-containing protein 1 isoform X1
XM_008854395.2	XP_008852617.1	PHD and RING finger domain-containing protein 1 isoform X2
XM_008854395.3	XP_008852617.1	PHD and RING finger domain-containing protein 1 isoform X3
XM_017803574.1	XP_017659063.1	PHD and RING finger domain-containing protein 1 isoform X3
XM_008854394.3	XP_008852616.1	PHD and RING finger domain-containing protein 1 isoform X2
XM_008854394.2	XP_008852616.1	PHD and RING finger domain-containing protein 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following Phrf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Phrf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONa137136	XM_029556781.1 Latest version!	Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ONa36152	XM_008854394.3 Latest version!	Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ONa36153	XM_008854395.3 Latest version!	Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ONa36154	XM_017803574.1 Latest version!	Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
View Old Accession Versions >>
ONa36153	XM_008854395.2	Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ONa36152	XM_008854394.2	Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Phrf1 ( XM_029556781.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029412641.1 Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X1, mRNA.

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CloneID	ONa137136
Clone ID Related Accession (Same CDS sequence)	XM_029556781.1
Accession Version	XM_029556781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	PHD and RING finger domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008356261.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGTTTGGA TGAGCTTGTG GCCCACAGCC CTGGGCCAAA TGGACCTCCA 
AGAGTCGTCC CCTCAGAACT GGCCAGTGAA GCTGCGTGGT CCTGGCTCTC TGCTGCCTGG 
GTTGTGACAC TGCTTCCTGG GGCCTATGTT TTTTCACTTT GTGGTCTAGA AGAAAGTGGC 
AATGGTCACA GTGGAGACTC TGAAGACTAC AGTGGCAGTG AGCATGAAGA CAACACAGAT 
GGGGAAGAAA CAGAGGGGTT GTCTGAGGAG GAAGACCCAG AAGATAGATC TGGTTCTGAA 
GATTCAGAAG ATGGAATAGA AACATCAGTG GCAGCAGTGG GTACTCAAGG GAAGCTGGAA 
ACTAGCAGCG CACTCAATTC TGATGATGAT GCAGAGAGCT GCCCTATTTG CCTCAATGCA 
TTCAGAGACC AGGCTGTGGG CACTCCAGAG ACCTGTGCCC ACTATTTCTG CTTGGACTGT 
ATTGTCGAAT GGTCTAGGAA TGCCAACTCC TGTCCAGTTG ATCGAACAGT ATTTAAATGT 
ATTTGTATTC GAGCTCGACT TGATGGTAAA ATTTTAAAGA AGATTCCAGT GGAAAACACT 
AGAGCCCGTG AGGATGAGGA GGCTGAGGAG GAGGACCCGA CCTTCTGTGA GGTGTGTGGT 
AGGAGTGACC GTGAGGACCG GCTCCTGCTC TGTGATGGCT GTGATGCTGG GTACCACATG 
GAATGTTTAG ATCCTCCTCT CCAGGAGGTA CCTGTGGATG AGTGGTTCTG CCCAGAGTGT 
GTCACCCCTG GTGCTGCCTC CACTCATGAC ACAGCTCCTG TGAGTGATGA GGAGGTCTCA 
CTGTTACTGG CCGACGTGGT ACCCACCACC AGCAGGCTGC GGCCTCGTGT GGGCAGGACC 
CGAGCCATCG CCAGGACACG ACAGAGTGAG AGAGTGAGGG CCACTGTAAA CCGGAACAGG 
ATCTCATCTG CCAGAAGGGT TCAGCATGTG CCAAGATACC TCATGTCTTC TCTGCTGGAT 
GAGACTATCG AGGCTGTCGC CACTGGTCTG AGCACTGCTG TGTACCAGCG CTCCCTGACT 
CCTCGTGTCC CTGCAAAACG GAAAAGGAAG GCAGGGAGAC GGAAAAAAGT GCTTGGAAGA 
AAGACGACCC GGTCTAGGTC ATCTGTGAAA AGGAAGAGTG GGGGTCTGAG AGCCAAGAAA 
CGCCAGCACC GTGTGAGGAA GACAAAAGGG AGAAAGATGA AGAATGAGGT TACAGCTCGT 
TCTCGAATTG CACGGACACT GGGCCTCCGC AGGCCTGTCC GTGGCACCAG CATGCCATCA 
GTGTACAAAC CAGTAGACCC CTCCCTGGGG CTAATGAGGG CAGATATCGG AGCAGCTTCT 
CTGTCACTGT TTGGAGACCC CTACGAGCTG GACCCTTTTG ACAGCAATGA AGAACTGTCT 
GCAAATCTGC CTTCCCCTCT GAGTGCCAAG AGGAGAGTGC TGTCCCGCTC GGCCTTGCAG 
TCTCACCAGC CTGTAGCCAG GCCTGTTGCT ATGGGGCTCT CCAGAAGGCA GCTCCCAGCT 
GTGGCCCCAG AGCCCAGTGT GGAGGAGGCT CCTGTTCCTG ATCTGTTGGG GAGCATCCTG 
ACTGGCCAGA GCCTCCTGAT GATGAGCAGC GCTGATGTTG TCATCCACCG GGATGGCTCT 
CTTAGCGCAA AGAGGACAGC TCCTGTTTCT CTTCAACGAA ATTCTGTGAC TCAATCCAGA 
GGAGAAGATG AGTCCAGGCG CAGAGACAGC CTACAGCCTG AGGCATCACC CTCAGGGAGC 
CCATCCAATG TCGGACTCAC AGGAAGCAGG CCACAGAGCT CAGGGATAAG CTGTGGGGAC 
AGGTCGGCCC TACGCTGTAT CCCCGCCTAC TCAGTGGGGA CACCTGCGAG GCCGGAGTCA 
TCATCAACCC CTGGTTCAAG TCAGGCTGGG AAGCTTTCAA ATGGGAGCAG GCCTAGCCTG 
AAGCAGAATA ACAGCCCCCA GTTTAATGGC GCGAACATGC AGGCCTTGCC TCTTCGTTCT 
GCCTCCTCTA AGATCTCAGC CACTCACTCT AACTCTTCAT CTAGAAATAT AGTCCTTAGA 
CAGTCCCTGA AAACAGCTCC CAGGAGAGCC GACATCTCAC AGTTGCCCAG AATACCAAAG 
ATCCGTAGGA ATGGCAGTGG CAGCCGACAA GACCCTGCCC CAGCCCATGG GCAGAGCGTG 
GAGCTCCCCA GCACCTGCAT TAGCCGCCTG ACAGGAAGGG AGGGTGCTGG ACAGCCAGGG 
CGAGGCCGAG CTGAGAATGA GCCCAGAAAC AGGGGCCCTC AGGAGCCTGG TTCACACACG 
GGTGGCTCTC GGCCTCCTGC CCCCAGCTCC CATGGCAACT TGGCCCCTCT AGGGCCTTCA 
AGAGGGAAAG GCATTGGGTC AACCTTTGAG AGTTTCAGGA TCAACATCCC CGGGAATACT 
GCACACAGCA GCCAGCTGTC CAGCCCTGGC TTCTGTAACA CATTCCGGCC AGTGGATAGC 
AAAGTGCAGA GGAAGGAGAA CCCTTCGCCA CTCTTCTCCA TCAAGAAGCC GAAGCAACTC 
AAGAGTGAAA TCTATGACCC CTTTGACCCC ACTGGCTCTG ACTCTAGTCC TCCTACCAGC 
AGCCCAGAGA GTATTGGCCC AGGCCTTCTG CCATCTGAGA TCACGAGAAC AATCTCCATC 
AACAGCCCAA AAGCCCCAGC CTTCCAGGCT GTGCGCTGTG TTACCTCCTA CAGGGTGGAA 
AGCATCTTTG ATACTGAGGT GGAACCTGAG CCCCAGCCCC CTGGTGAGCC CATGTCTGGC 
ATGTTCAAGT TCCTGGGCAA GGGGCCTGCT GAGGGAGCCT CTGACTTGGA GCAAGAAAGG 
CTAGGTGAGG TGGAGCATCC AGAAATCGAG GGTTCCATAG CCCAGACACA CAGGCCATCC 
CCACAAGACC CCTGGGAAGA TGAGGATGGA ATGTCCTGTA CACCCTTCTT TGGTTCTGAG 
GAGCGGACAG TGACATGTGT GACTGTCATG GAGCCAGATG TTCCACAGGT CACCACACAT 
AGGATTGTAG AGCTGAGAAC CCCATCCCGA TCCCGTTCCA CCTCTAGCTC CCGAAGCAAG 
AAGAAAACCA AGAAGAAGAG GGCTGCCAGG GAGCACCAGA GGACACGCTC TAGCACTCGC 
TCTGGCTCCA GGGACAGGAC CTCACGTTCG GTATCGCCCG TGGTCGAGGA GCACACCAAG 
AGGCACAGGG CCAAGACCAA GAGCCGGAGG TCTTCCAGCG ACCGTGCCAG CAGCCAGGAG 
AGAGTGAAGA GGAGGAAGAC CAGGGACAGG GACAGGGAGC GGAGACGGGG CTCCTGGAGC 
CATGGCAGGT GCCGCAGGAA GTCCAGGTCT CACTCAGGCA GTCCTGGCAG TTCTTCCTGT 
GAGCGCCATG AAAGCAGGAA GCGGAAGAGG CGACGCTCAG GCTCCAGGTC TCGGGGCAAG 
GACTGCTCTT CCCCCAGCAA CCTAGAGCGG GCCAGGAGAC ACAAGCACCG GGAACGGAGC 
CGGAGCCGAG ACAAGAAAGA CAGTATGACC TGGTCCCGGG AGAGGAGGAG GTGGAGGTCT 
AGATCACCAA GCTTGGAGCA TAGGCCACGG CGGCCACGTT CCCGAGAGAA GCGGCCCCGT 
TCCCCAGAGA AGGCTCCAGC TGTGAAGGAA GCTTCCCCAG CCCCCACTGC TCAGGAGGAG 
CTGAGGCAAG ATGAAGACCG CCCGGCTAAA CCTTCAGTCT CCGAGGAAGT GAGTGTCCTG 
CCAGAGGTGG TTGTGGCTGA CCTGAACCCC CCAGAAATGC CCCCTGTCCT GGCAGTGCCA 
GCTGAATCTG TGCCTGAGGA CTTGGACTAT GGTGACTCTG TGGAAGCAGG GCACGTCTTT 
GAGGATTTTT CAAGTGAAGC CATCTTTATC CAGCTTGATG ACATGAGTTC ACCTCCTTCC 
CCTGAGAGTA CAGACTCCTC CCCAGAACGA GACTTCCCAC CAAATCCTGT GGTGCCCCCA 
GCCATCCAGC CACAGGATGC CAGTCTGGCT GCCATCCAGA GGGAAGTGTC CCTGATCCAC 
AGTGAAGACA TCTCTCAGCC CACACCACAG GCAGAGGGCC CTTCAGACCA GTACCTGCTC 
AGGCAGGACG CTGTAGAGAC CATTGCCATG ACTCCCAGCA CCATGGGTGT GGTACCCATG 
GGAAAAGACA GCCCCTTGCT GAGTGGGAGA GGGTGTGAAG CAGTCAGGTC CAAGGAGACA 
GTGGTCCAGG CCCCTCTGCT ACGGGCCAGG GCCCTTGTAA AAAGAGTCAC CTGGAACCTA 
CAGGAGGCAG AGGATGGTAC TCCAGCCCTG GACAGAGTTC CAAGGACACC ACTTCAGAGG 
CCACAGAGGC CCCGGGAAGG AGACTGGGAT GCAGAGGACG GGGCCCTCGT AGGAGTTCAG 
CAGGCACCAT TCTCCGAGCT GCCCCCTCCC ATCCACGTGC TCCAGGAGTC TGGGTTACCT 
GATGCAGACC CCTCTCAGCC CCCTGGTGCT TCCAGGGCAG AAGAGGTGCC AGCTGTTGGG 
ACTCTGCACT CAGCAGAGGG TATTCTTGCC CAAGTTTACA ACCCCAACAT GCCACCACCC 
CTGGCTCAGC CATCAAGCAT CCTGCCCTAC ACCCTCGTCA GTCAGCCCTC GGTCCAGTTA 
ATCCTGCAGG GGACCCTTCC CCTGGCTGGC TGTGGCACAG CACAGACCCT GGCCCCAGTG 
CCTAATGTTC CAGCTGTAGC CTCAGAGCTG GCTGTTCTAA CCAACAACAG CAGCTCTGAG 
GAGAGGACCA GCACTCCCAA GCCAGCCGCC GAGAAGACCA AGAAGGAAGA GTACTTGAAG 
AAGCTGCACA TGCAGGAGCG GGCTGTGGAG GAGGTGAAGC TGGCCATTAA GCCCTTCTAC 
CAGAAGAGAG AGGTGACCAA GGAGGAATAC AAGGACATCC TGCGGAAAGC TGTGCAGAAG 
ATCTGCCACA GCAAGAGCGG CGAGATCAAC CCTGTGAAGG TGGCCAATCT GGTAAAGGCC 
TATGTGGACA AGTATAGGCA CATGCGCAGG CACAAGAAGA CTGAAGCTGG GGAGGAGCCA 
CCTGCCCAGG GTGCCGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029412641.1
CDS	388..5511
Translation	MDDDSLDELVAHSPGPNGPPRVVPSELASEAAWSWLSAAWVVTLLPGAYVFSLCGLEESGNGHSGDSEDYSGSEHEDNTDGEETEGLSEEEDPEDRSGSEDSEDGIETSVAAVGTQGKLETSSALNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIVEWSRNANSCPVDRTVFKCICIRARLDGKILKKIPVENTRAREDEEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECVTPGAASTHDTAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRSLTPRVPAKRKRKAGRRKKVLGRKTTRSRSSVKRKSGGLRAKKRQHRVRKTKGRKMKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEELSANLPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILTGQSLLMMSSADVVIHRDGSLSAKRTAPVSLQRNSVTQSRGEDESRRRDSLQPEASPSGSPSNVGLTGSRPQSSGISCGDRSALRCIPAYSVGTPARPESSSTPGSSQAGKLSNGSRPSLKQNNSPQFNGANMQALPLRSASSKISATHSNSSSRNIVLRQSLKTAPRRADISQLPRIPKIRRNGSGSRQDPAPAHGQSVELPSTCISRLTGREGAGQPGRGRAENEPRNRGPQEPGSHTGGSRPPAPSSHGNLAPLGPSRGKGIGSTFESFRINIPGNTAHSSQLSSPGFCNTFRPVDSKVQRKENPSPLFSIKKPKQLKSEIYDPFDPTGSDSSPPTSSPESIGPGLLPSEITRTISINSPKAPAFQAVRCVTSYRVESIFDTEVEPEPQPPGEPMSGMFKFLGKGPAEGASDLEQERLGEVEHPEIEGSIAQTHRPSPQDPWEDEDGMSCTPFFGSEERTVTCVTVMEPDVPQVTTHRIVELRTPSRSRSTSSSRSKKKTKKKRAAREHQRTRSSTRSGSRDRTSRSVSPVVEEHTKRHRAKTKSRRSSSDRASSQERVKRRKTRDRDRERRRGSWSHGRCRRKSRSHSGSPGSSSCERHESRKRKRRRSGSRSRGKDCSSPSNLERARRHKHRERSRSRDKKDSMTWSRERRRWRSRSPSLEHRPRRPRSREKRPRSPEKAPAVKEASPAPTAQEELRQDEDRPAKPSVSEEVSVLPEVVVADLNPPEMPPVLAVPAESVPEDLDYGDSVEAGHVFEDFSSEAIFIQLDDMSSPPSPESTDSSPERDFPPNPVVPPAIQPQDASLAAIQREVSLIHSEDISQPTPQAEGPSDQYLLRQDAVETIAMTPSTMGVVPMGKDSPLLSGRGCEAVRSKETVVQAPLLRARALVKRVTWNLQEAEDGTPALDRVPRTPLQRPQRPREGDWDAEDGALVGVQQAPFSELPPPIHVLQESGLPDADPSQPPGASRAEEVPAVGTLHSAEGILAQVYNPNMPPPLAQPSSILPYTLVSQPSVQLILQGTLPLAGCGTAQTLAPVPNVPAVASELAVLTNNSSSEERTSTPKPAAEKTKKEEYLKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEAGEEPPAQGAET

Target ORF information:

RefSeq Version	XM_029556781.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029556781.1

ORF Insert Sequence:

ATGGATGACG ACAGTTTGGA TGAGCTTGTG GCCCACAGCC CTGGGCCAAA TGGACCTCCA 
AGAGTCGTCC CCTCAGAACT GGCCAGTGAA GCTGCGTGGT CCTGGCTCTC TGCTGCCTGG 
GTTGTGACAC TGCTTCCTGG GGCCTATGTT TTTTCACTTT GTGGTCTAGA AGAAAGTGGC 
AATGGTCACA GTGGAGACTC TGAAGACTAC AGTGGCAGTG AGCATGAAGA CAACACAGAT 
GGGGAAGAAA CAGAGGGGTT GTCTGAGGAG GAAGACCCAG AAGATAGATC TGGTTCTGAA 
GATTCAGAAG ATGGAATAGA AACATCAGTG GCAGCAGTGG GTACTCAAGG GAAGCTGGAA 
ACTAGCAGCG CACTCAATTC TGATGATGAT GCAGAGAGCT GCCCTATTTG CCTCAATGCA 
TTCAGAGACC AGGCTGTGGG CACTCCAGAG ACCTGTGCCC ACTATTTCTG CTTGGACTGT 
ATTGTCGAAT GGTCTAGGAA TGCCAACTCC TGTCCAGTTG ATCGAACAGT ATTTAAATGT 
ATTTGTATTC GAGCTCGACT TGATGGTAAA ATTTTAAAGA AGATTCCAGT GGAAAACACT 
AGAGCCCGTG AGGATGAGGA GGCTGAGGAG GAGGACCCGA CCTTCTGTGA GGTGTGTGGT 
AGGAGTGACC GTGAGGACCG GCTCCTGCTC TGTGATGGCT GTGATGCTGG GTACCACATG 
GAATGTTTAG ATCCTCCTCT CCAGGAGGTA CCTGTGGATG AGTGGTTCTG CCCAGAGTGT 
GTCACCCCTG GTGCTGCCTC CACTCATGAC ACAGCTCCTG TGAGTGATGA GGAGGTCTCA 
CTGTTACTGG CCGACGTGGT ACCCACCACC AGCAGGCTGC GGCCTCGTGT GGGCAGGACC 
CGAGCCATCG CCAGGACACG ACAGAGTGAG AGAGTGAGGG CCACTGTAAA CCGGAACAGG 
ATCTCATCTG CCAGAAGGGT TCAGCATGTG CCAAGATACC TCATGTCTTC TCTGCTGGAT 
GAGACTATCG AGGCTGTCGC CACTGGTCTG AGCACTGCTG TGTACCAGCG CTCCCTGACT 
CCTCGTGTCC CTGCAAAACG GAAAAGGAAG GCAGGGAGAC GGAAAAAAGT GCTTGGAAGA 
AAGACGACCC GGTCTAGGTC ATCTGTGAAA AGGAAGAGTG GGGGTCTGAG AGCCAAGAAA 
CGCCAGCACC GTGTGAGGAA GACAAAAGGG AGAAAGATGA AGAATGAGGT TACAGCTCGT 
TCTCGAATTG CACGGACACT GGGCCTCCGC AGGCCTGTCC GTGGCACCAG CATGCCATCA 
GTGTACAAAC CAGTAGACCC CTCCCTGGGG CTAATGAGGG CAGATATCGG AGCAGCTTCT 
CTGTCACTGT TTGGAGACCC CTACGAGCTG GACCCTTTTG ACAGCAATGA AGAACTGTCT 
GCAAATCTGC CTTCCCCTCT GAGTGCCAAG AGGAGAGTGC TGTCCCGCTC GGCCTTGCAG 
TCTCACCAGC CTGTAGCCAG GCCTGTTGCT ATGGGGCTCT CCAGAAGGCA GCTCCCAGCT 
GTGGCCCCAG AGCCCAGTGT GGAGGAGGCT CCTGTTCCTG ATCTGTTGGG GAGCATCCTG 
ACTGGCCAGA GCCTCCTGAT GATGAGCAGC GCTGATGTTG TCATCCACCG GGATGGCTCT 
CTTAGCGCAA AGAGGACAGC TCCTGTTTCT CTTCAACGAA ATTCTGTGAC TCAATCCAGA 
GGAGAAGATG AGTCCAGGCG CAGAGACAGC CTACAGCCTG AGGCATCACC CTCAGGGAGC 
CCATCCAATG TCGGACTCAC AGGAAGCAGG CCACAGAGCT CAGGGATAAG CTGTGGGGAC 
AGGTCGGCCC TACGCTGTAT CCCCGCCTAC TCAGTGGGGA CACCTGCGAG GCCGGAGTCA 
TCATCAACCC CTGGTTCAAG TCAGGCTGGG AAGCTTTCAA ATGGGAGCAG GCCTAGCCTG 
AAGCAGAATA ACAGCCCCCA GTTTAATGGC GCGAACATGC AGGCCTTGCC TCTTCGTTCT 
GCCTCCTCTA AGATCTCAGC CACTCACTCT AACTCTTCAT CTAGAAATAT AGTCCTTAGA 
CAGTCCCTGA AAACAGCTCC CAGGAGAGCC GACATCTCAC AGTTGCCCAG AATACCAAAG 
ATCCGTAGGA ATGGCAGTGG CAGCCGACAA GACCCTGCCC CAGCCCATGG GCAGAGCGTG 
GAGCTCCCCA GCACCTGCAT TAGCCGCCTG ACAGGAAGGG AGGGTGCTGG ACAGCCAGGG 
CGAGGCCGAG CTGAGAATGA GCCCAGAAAC AGGGGCCCTC AGGAGCCTGG TTCACACACG 
GGTGGCTCTC GGCCTCCTGC CCCCAGCTCC CATGGCAACT TGGCCCCTCT AGGGCCTTCA 
AGAGGGAAAG GCATTGGGTC AACCTTTGAG AGTTTCAGGA TCAACATCCC CGGGAATACT 
GCACACAGCA GCCAGCTGTC CAGCCCTGGC TTCTGTAACA CATTCCGGCC AGTGGATAGC 
AAAGTGCAGA GGAAGGAGAA CCCTTCGCCA CTCTTCTCCA TCAAGAAGCC GAAGCAACTC 
AAGAGTGAAA TCTATGACCC CTTTGACCCC ACTGGCTCTG ACTCTAGTCC TCCTACCAGC 
AGCCCAGAGA GTATTGGCCC AGGCCTTCTG CCATCTGAGA TCACGAGAAC AATCTCCATC 
AACAGCCCAA AAGCCCCAGC CTTCCAGGCT GTGCGCTGTG TTACCTCCTA CAGGGTGGAA 
AGCATCTTTG ATACTGAGGT GGAACCTGAG CCCCAGCCCC CTGGTGAGCC CATGTCTGGC 
ATGTTCAAGT TCCTGGGCAA GGGGCCTGCT GAGGGAGCCT CTGACTTGGA GCAAGAAAGG 
CTAGGTGAGG TGGAGCATCC AGAAATCGAG GGTTCCATAG CCCAGACACA CAGGCCATCC 
CCACAAGACC CCTGGGAAGA TGAGGATGGA ATGTCCTGTA CACCCTTCTT TGGTTCTGAG 
GAGCGGACAG TGACATGTGT GACTGTCATG GAGCCAGATG TTCCACAGGT CACCACACAT 
AGGATTGTAG AGCTGAGAAC CCCATCCCGA TCCCGTTCCA CCTCTAGCTC CCGAAGCAAG 
AAGAAAACCA AGAAGAAGAG GGCTGCCAGG GAGCACCAGA GGACACGCTC TAGCACTCGC 
TCTGGCTCCA GGGACAGGAC CTCACGTTCG GTATCGCCCG TGGTCGAGGA GCACACCAAG 
AGGCACAGGG CCAAGACCAA GAGCCGGAGG TCTTCCAGCG ACCGTGCCAG CAGCCAGGAG 
AGAGTGAAGA GGAGGAAGAC CAGGGACAGG GACAGGGAGC GGAGACGGGG CTCCTGGAGC 
CATGGCAGGT GCCGCAGGAA GTCCAGGTCT CACTCAGGCA GTCCTGGCAG TTCTTCCTGT 
GAGCGCCATG AAAGCAGGAA GCGGAAGAGG CGACGCTCAG GCTCCAGGTC TCGGGGCAAG 
GACTGCTCTT CCCCCAGCAA CCTAGAGCGG GCCAGGAGAC ACAAGCACCG GGAACGGAGC 
CGGAGCCGAG ACAAGAAAGA CAGTATGACC TGGTCCCGGG AGAGGAGGAG GTGGAGGTCT 
AGATCACCAA GCTTGGAGCA TAGGCCACGG CGGCCACGTT CCCGAGAGAA GCGGCCCCGT 
TCCCCAGAGA AGGCTCCAGC TGTGAAGGAA GCTTCCCCAG CCCCCACTGC TCAGGAGGAG 
CTGAGGCAAG ATGAAGACCG CCCGGCTAAA CCTTCAGTCT CCGAGGAAGT GAGTGTCCTG 
CCAGAGGTGG TTGTGGCTGA CCTGAACCCC CCAGAAATGC CCCCTGTCCT GGCAGTGCCA 
GCTGAATCTG TGCCTGAGGA CTTGGACTAT GGTGACTCTG TGGAAGCAGG GCACGTCTTT 
GAGGATTTTT CAAGTGAAGC CATCTTTATC CAGCTTGATG ACATGAGTTC ACCTCCTTCC 
CCTGAGAGTA CAGACTCCTC CCCAGAACGA GACTTCCCAC CAAATCCTGT GGTGCCCCCA 
GCCATCCAGC CACAGGATGC CAGTCTGGCT GCCATCCAGA GGGAAGTGTC CCTGATCCAC 
AGTGAAGACA TCTCTCAGCC CACACCACAG GCAGAGGGCC CTTCAGACCA GTACCTGCTC 
AGGCAGGACG CTGTAGAGAC CATTGCCATG ACTCCCAGCA CCATGGGTGT GGTACCCATG 
GGAAAAGACA GCCCCTTGCT GAGTGGGAGA GGGTGTGAAG CAGTCAGGTC CAAGGAGACA 
GTGGTCCAGG CCCCTCTGCT ACGGGCCAGG GCCCTTGTAA AAAGAGTCAC CTGGAACCTA 
CAGGAGGCAG AGGATGGTAC TCCAGCCCTG GACAGAGTTC CAAGGACACC ACTTCAGAGG 
CCACAGAGGC CCCGGGAAGG AGACTGGGAT GCAGAGGACG GGGCCCTCGT AGGAGTTCAG 
CAGGCACCAT TCTCCGAGCT GCCCCCTCCC ATCCACGTGC TCCAGGAGTC TGGGTTACCT 
GATGCAGACC CCTCTCAGCC CCCTGGTGCT TCCAGGGCAG AAGAGGTGCC AGCTGTTGGG 
ACTCTGCACT CAGCAGAGGG TATTCTTGCC CAAGTTTACA ACCCCAACAT GCCACCACCC 
CTGGCTCAGC CATCAAGCAT CCTGCCCTAC ACCCTCGTCA GTCAGCCCTC GGTCCAGTTA 
ATCCTGCAGG GGACCCTTCC CCTGGCTGGC TGTGGCACAG CACAGACCCT GGCCCCAGTG 
CCTAATGTTC CAGCTGTAGC CTCAGAGCTG GCTGTTCTAA CCAACAACAG CAGCTCTGAG 
GAGAGGACCA GCACTCCCAA GCCAGCCGCC GAGAAGACCA AGAAGGAAGA GTACTTGAAG 
AAGCTGCACA TGCAGGAGCG GGCTGTGGAG GAGGTGAAGC TGGCCATTAA GCCCTTCTAC 
CAGAAGAGAG AGGTGACCAA GGAGGAATAC AAGGACATCC TGCGGAAAGC TGTGCAGAAG 
ATCTGCCACA GCAAGAGCGG CGAGATCAAC CCTGTGAAGG TGGCCAATCT GGTAAAGGCC 
TATGTGGACA AGTATAGGCA CATGCGCAGG CACAAGAAGA CTGAAGCTGG GGAGGAGCCA 
CCTGCCCAGG GTGCCGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_008854394.3 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008852616.1 Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONa36152
Clone ID Related Accession (Same CDS sequence)	XM_008854394.3 , XM_008854394.2
Accession Version	XM_008854394.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	PHD and RING finger domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008356261.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008854394.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGTTTGGA TGAGCTTGTG GCCCACAGCC CTGGGCCAAA TGGACCTCCA 
AGAGTCGTCC CCTCAGAACT GGCCAGTGAA GCTGAAGAAA GTGGCAATGG TCACAGTGGA 
GACTCTGAAG ACTACAGTGG CAGTGAGCAT GAAGACAACA CAGATGGGGA AGAAACAGAG 
GGGTTGTCTG AGGAGGAAGA CCCAGAAGAT AGATCTGGTT CTGAAGATTC AGAAGATGGA 
ATAGAAACAT CAGTGGCAGC AGTGGGTACT CAAGGGAAGC TGGAAACTAG CAGCGCACTC 
AATTCTGATG ATGATGCAGA GAGCTGCCCT ATTTGCCTCA ATGCATTCAG AGACCAGGCT 
GTGGGCACTC CAGAGACCTG TGCCCACTAT TTCTGCTTGG ACTGTATTGT CGAATGGTCT 
AGGAATGCCA ACTCCTGTCC AGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGAGCT 
CGACTTGATG GTAAAATTTT AAAGAAGATT CCAGTGGAAA ACACTAGAGC CCGTGAGGAT 
GAGGAGGCTG AGGAGGAGGA CCCGACCTTC TGTGAGGTGT GTGGTAGGAG TGACCGTGAG 
GACCGGCTCC TGCTCTGTGA TGGCTGTGAT GCTGGGTACC ACATGGAATG TTTAGATCCT 
CCTCTCCAGG AGGTACCTGT GGATGAGTGG TTCTGCCCAG AGTGTGTCAC CCCTGGTGCT 
GCCTCCACTC ATGACACAGC TCCTGTGAGT GATGAGGAGG TCTCACTGTT ACTGGCCGAC 
GTGGTACCCA CCACCAGCAG GCTGCGGCCT CGTGTGGGCA GGACCCGAGC CATCGCCAGG 
ACACGACAGA GTGAGAGAGT GAGGGCCACT GTAAACCGGA ACAGGATCTC ATCTGCCAGA 
AGGGTTCAGC ATGTGCCAAG ATACCTCATG TCTTCTCTGC TGGATGAGAC TATCGAGGCT 
GTCGCCACTG GTCTGAGCAC TGCTGTGTAC CAGCGCTCCC TGACTCCTCG TGTCCCTGCA 
AAACGGAAAA GGAAGGCAGG GAGACGGAAA AAAGTGCTTG GAAGAAAGAC GACCCGGTCT 
AGGTCATCTG TGAAAAGGAA GAGTGGGGGT CTGAGAGCCA AGAAACGCCA GCACCGTGTG 
AGGAAGACAA AAGGGAGAAA GATGAAGAAT GAGGTTACAG CTCGTTCTCG AATTGCACGG 
ACACTGGGCC TCCGCAGGCC TGTCCGTGGC ACCAGCATGC CATCAGTGTA CAAACCAGTA 
GACCCCTCCC TGGGGCTAAT GAGGGCAGAT ATCGGAGCAG CTTCTCTGTC ACTGTTTGGA 
GACCCCTACG AGCTGGACCC TTTTGACAGC AATGAAGAAC TGTCTGCAAA TCTGCCTTCC 
CCTCTGAGTG CCAAGAGGAG AGTGCTGTCC CGCTCGGCCT TGCAGTCTCA CCAGCCTGTA 
GCCAGGCCTG TTGCTATGGG GCTCTCCAGA AGGCAGCTCC CAGCTGTGGC CCCAGAGCCC 
AGTGTGGAGG AGGCTCCTGT TCCTGATCTG TTGGGGAGCA TCCTGACTGG CCAGAGCCTC 
CTGATGATGA GCAGCGCTGA TGTTGTCATC CACCGGGATG GCTCTCTTAG CGCAAAGAGG 
ACAGCTCCTG TTTCTCTTCA ACGAAATTCT GTGACTCAAT CCAGAGGAGA AGATGAGTCC 
AGGCGCAGAG ACAGCCTACA GCCTGAGGCA TCACCCTCAG GGAGCCCATC CAATGTCGGA 
CTCACAGGAA GCAGGCCACA GAGCTCAGGG ATAAGCTGTG GGGACAGGTC GGCCCTACGC 
TGTATCCCCG CCTACTCAGT GGGGACACCT GCGAGGCCGG AGTCATCATC AACCCCTGGT 
TCAAGTCAGG CTGGGAAGCT TTCAAATGGG AGCAGGCCTA GCCTGAAGCA GAATAACAGC 
CCCCAGTTTA ATGGCGCGAA CATGCAGGCC TTGCCTCTTC GTTCTGCCTC CTCTAAGATC 
TCAGCCACTC ACTCTAACTC TTCATCTAGA AATATAGTCC TTAGACAGTC CCTGAAAACA 
GCTCCCAGGA GAGCCGACAT CTCACAGTTG CCCAGAATAC CAAAGATCCG TAGGAATGGC 
AGTGGCAGCC GACAAGACCC TGCCCCAGCC CATGGGCAGA GCGTGGAGCT CCCCAGCACC 
TGCATTAGCC GCCTGACAGG AAGGGAGGGT GCTGGACAGC CAGGGCGAGG CCGAGCTGAG 
AATGAGCCCA GAAACAGGGG CCCTCAGGAG CCTGGTTCAC ACACGGGTGG CTCTCGGCCT 
CCTGCCCCCA GCTCCCATGG CAACTTGGCC CCTCTAGGGC CTTCAAGAGG GAAAGGCATT 
GGGTCAACCT TTGAGAGTTT CAGGATCAAC ATCCCCGGGA ATACTGCACA CAGCAGCCAG 
CTGTCCAGCC CTGGCTTCTG TAACACATTC CGGCCAGTGG ATAGCAAAGT GCAGAGGAAG 
GAGAACCCTT CGCCACTCTT CTCCATCAAG AAGCCGAAGC AACTCAAGAG TGAAATCTAT 
GACCCCTTTG ACCCCACTGG CTCTGACTCT AGTCCTCCTA CCAGCAGCCC AGAGAGTATT 
GGCCCAGGCC TTCTGCCATC TGAGATCACG AGAACAATCT CCATCAACAG CCCAAAAGCC 
CCAGCCTTCC AGGCTGTGCG CTGTGTTACC TCCTACAGGG TGGAAAGCAT CTTTGATACT 
GAGGTGGAAC CTGAGCCCCA GCCCCCTGGT GAGCCCATGT CTGGCATGTT CAAGTTCCTG 
GGCAAGGGGC CTGCTGAGGG AGCCTCTGAC TTGGAGCAAG AAAGGCTAGG TGAGGTGGAG 
CATCCAGAAA TCGAGGGTTC CATAGCCCAG ACACACAGGC CATCCCCACA AGACCCCTGG 
GAAGATGAGG ATGGAATGTC CTGTACACCC TTCTTTGGTT CTGAGGAGCG GACAGTGACA 
TGTGTGACTG TCATGGAGCC AGATGTTCCA CAGGTCACCA CACATAGGAT TGTAGAGCTG 
AGAACCCCAT CCCGATCCCG TTCCACCTCT AGCTCCCGAA GCAAGAAGAA AACCAAGAAG 
AAGAGGGCTG CCAGGGAGCA CCAGAGGACA CGCTCTAGCA CTCGCTCTGG CTCCAGGGAC 
AGGACCTCAC GTTCGGTATC GCCCGTGGTC GAGGAGCACA CCAAGAGGCA CAGGGCCAAG 
ACCAAGAGCC GGAGGTCTTC CAGCGACCGT GCCAGCAGCC AGGAGAGAGT GAAGAGGAGG 
AAGACCAGGG ACAGGGACAG GGAGCGGAGA CGGGGCTCCT GGAGCCATGG CAGGTGCCGC 
AGGAAGTCCA GGTCTCACTC AGGCAGTCCT GGCAGTTCTT CCTGTGAGCG CCATGAAAGC 
AGGAAGCGGA AGAGGCGACG CTCAGGCTCC AGGTCTCGGG GCAAGGACTG CTCTTCCCCC 
AGCAACCTAG AGCGGGCCAG GAGACACAAG CACCGGGAAC GGAGCCGGAG CCGAGACAAG 
AAAGACAGTA TGACCTGGTC CCGGGAGAGG AGGAGGTGGA GGTCTAGATC ACCAAGCTTG 
GAGCATAGGC CACGGCGGCC ACGTTCCCGA GAGAAGCGGC CCCGTTCCCC AGAGAAGGCT 
CCAGCTGTGA AGGAAGCTTC CCCAGCCCCC ACTGCTCAGG AGGAGCTGAG GCAAGATGAA 
GACCGCCCGG CTAAACCTTC AGTCTCCGAG GAAGTGAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCCCCAGA AATGCCCCCT GTCCTGGCAG TGCCAGCTGA ATCTGTGCCT 
GAGGACTTGG ACTATGGTGA CTCTGTGGAA GCAGGGCACG TCTTTGAGGA TTTTTCAAGT 
GAAGCCATCT TTATCCAGCT TGATGACATG AGTTCACCTC CTTCCCCTGA GAGTACAGAC 
TCCTCCCCAG AACGAGACTT CCCACCAAAT CCTGTGGTGC CCCCAGCCAT CCAGCCACAG 
GATGCCAGTC TGGCTGCCAT CCAGAGGGAA GTGTCCCTGA TCCACAGTGA AGACATCTCT 
CAGCCCACAC CACAGGCAGA GGGCCCTTCA GACCAGTACC TGCTCAGGCA GGACGCTGTA 
GAGACCATTG CCATGACTCC CAGCACCATG GGTGTGGTAC CCATGGGAAA AGACAGCCCC 
TTGCTGAGTG GGAGAGGGTG TGAAGCAGTC AGGTCCAAGG AGACAGTGGT CCAGGCCCCT 
CTGCTACGGG CCAGGGCCCT TGTAAAAAGA GTCACCTGGA ACCTACAGGA GGCAGAGGAT 
GGTACTCCAG CCCTGGACAG AGTTCCAAGG ACACCACTTC AGAGGCCACA GAGGCCCCGG 
GAAGGAGACT GGGATGCAGA GGACGGGGCC CTCGTAGGAG TTCAGCAGGC ACCATTCTCC 
GAGCTGCCCC CTCCCATCCA CGTGCTCCAG GAGTCTGGGT TACCTGATGC AGACCCCTCT 
CAGCCCCCTG GTGCTTCCAG GGCAGAAGAG GTGCCAGCTG TTGGGACTCT GCACTCAGCA 
GAGGGTATTC TTGCCCAAGT TTACAACCCC AACATGCCAC CACCCCTGGC TCAGCCATCA 
AGCATCCTGC CCTACACCCT CGTCAGTCAG CCCTCGGTCC AGTTAATCCT GCAGGGGACC 
CTTCCCCTGG CTGGCTGTGG CACAGCACAG ACCCTGGCCC CAGTGCCTAA TGTTCCAGCT 
GTAGCCTCAG AGCTGGCTGT TCTAACCAAC AACAGCAGCT CTGAGGAGAG GACCAGCACT 
CCCAAGCCAG CCGCCGAGAA GACCAAGAAG GAAGAGTACT TGAAGAAGCT GCACATGCAG 
GAGCGGGCTG TGGAGGAGGT GAAGCTGGCC ATTAAGCCCT TCTACCAGAA GAGAGAGGTG 
ACCAAGGAGG AATACAAGGA CATCCTGCGG AAAGCTGTGC AGAAGATCTG CCACAGCAAG 
AGCGGCGAGA TCAACCCTGT GAAGGTGGCC AATCTGGTAA AGGCCTATGT GGACAAGTAT 
AGGCACATGC GCAGGCACAA GAAGACTGAA GCTGGGGAGG AGCCACCTGC CCAGGGTGCC 
GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008852616.1
CDS	387..5435
Translation	MDDDSLDELVAHSPGPNGPPRVVPSELASEAEESGNGHSGDSEDYSGSEHEDNTDGEETEGLSEEEDPEDRSGSEDSEDGIETSVAAVGTQGKLETSSALNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIVEWSRNANSCPVDRTVFKCICIRARLDGKILKKIPVENTRAREDEEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECVTPGAASTHDTAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRSLTPRVPAKRKRKAGRRKKVLGRKTTRSRSSVKRKSGGLRAKKRQHRVRKTKGRKMKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEELSANLPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILTGQSLLMMSSADVVIHRDGSLSAKRTAPVSLQRNSVTQSRGEDESRRRDSLQPEASPSGSPSNVGLTGSRPQSSGISCGDRSALRCIPAYSVGTPARPESSSTPGSSQAGKLSNGSRPSLKQNNSPQFNGANMQALPLRSASSKISATHSNSSSRNIVLRQSLKTAPRRADISQLPRIPKIRRNGSGSRQDPAPAHGQSVELPSTCISRLTGREGAGQPGRGRAENEPRNRGPQEPGSHTGGSRPPAPSSHGNLAPLGPSRGKGIGSTFESFRINIPGNTAHSSQLSSPGFCNTFRPVDSKVQRKENPSPLFSIKKPKQLKSEIYDPFDPTGSDSSPPTSSPESIGPGLLPSEITRTISINSPKAPAFQAVRCVTSYRVESIFDTEVEPEPQPPGEPMSGMFKFLGKGPAEGASDLEQERLGEVEHPEIEGSIAQTHRPSPQDPWEDEDGMSCTPFFGSEERTVTCVTVMEPDVPQVTTHRIVELRTPSRSRSTSSSRSKKKTKKKRAAREHQRTRSSTRSGSRDRTSRSVSPVVEEHTKRHRAKTKSRRSSSDRASSQERVKRRKTRDRDRERRRGSWSHGRCRRKSRSHSGSPGSSSCERHESRKRKRRRSGSRSRGKDCSSPSNLERARRHKHRERSRSRDKKDSMTWSRERRRWRSRSPSLEHRPRRPRSREKRPRSPEKAPAVKEASPAPTAQEELRQDEDRPAKPSVSEEVSVLPEVVVADLNPPEMPPVLAVPAESVPEDLDYGDSVEAGHVFEDFSSEAIFIQLDDMSSPPSPESTDSSPERDFPPNPVVPPAIQPQDASLAAIQREVSLIHSEDISQPTPQAEGPSDQYLLRQDAVETIAMTPSTMGVVPMGKDSPLLSGRGCEAVRSKETVVQAPLLRARALVKRVTWNLQEAEDGTPALDRVPRTPLQRPQRPREGDWDAEDGALVGVQQAPFSELPPPIHVLQESGLPDADPSQPPGASRAEEVPAVGTLHSAEGILAQVYNPNMPPPLAQPSSILPYTLVSQPSVQLILQGTLPLAGCGTAQTLAPVPNVPAVASELAVLTNNSSSEERTSTPKPAAEKTKKEEYLKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEAGEEPPAQGAET

Target ORF information:

RefSeq Version	XM_008854394.3
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008854394.3

ORF Insert Sequence:

ATGGATGACG ACAGTTTGGA TGAGCTTGTG GCCCACAGCC CTGGGCCAAA TGGACCTCCA 
AGAGTCGTCC CCTCAGAACT GGCCAGTGAA GCTGAAGAAA GTGGCAATGG TCACAGTGGA 
GACTCTGAAG ACTACAGTGG CAGTGAGCAT GAAGACAACA CAGATGGGGA AGAAACAGAG 
GGGTTGTCTG AGGAGGAAGA CCCAGAAGAT AGATCTGGTT CTGAAGATTC AGAAGATGGA 
ATAGAAACAT CAGTGGCAGC AGTGGGTACT CAAGGGAAGC TGGAAACTAG CAGCGCACTC 
AATTCTGATG ATGATGCAGA GAGCTGCCCT ATTTGCCTCA ATGCATTCAG AGACCAGGCT 
GTGGGCACTC CAGAGACCTG TGCCCACTAT TTCTGCTTGG ACTGTATTGT CGAATGGTCT 
AGGAATGCCA ACTCCTGTCC AGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGAGCT 
CGACTTGATG GTAAAATTTT AAAGAAGATT CCAGTGGAAA ACACTAGAGC CCGTGAGGAT 
GAGGAGGCTG AGGAGGAGGA CCCGACCTTC TGTGAGGTGT GTGGTAGGAG TGACCGTGAG 
GACCGGCTCC TGCTCTGTGA TGGCTGTGAT GCTGGGTACC ACATGGAATG TTTAGATCCT 
CCTCTCCAGG AGGTACCTGT GGATGAGTGG TTCTGCCCAG AGTGTGTCAC CCCTGGTGCT 
GCCTCCACTC ATGACACAGC TCCTGTGAGT GATGAGGAGG TCTCACTGTT ACTGGCCGAC 
GTGGTACCCA CCACCAGCAG GCTGCGGCCT CGTGTGGGCA GGACCCGAGC CATCGCCAGG 
ACACGACAGA GTGAGAGAGT GAGGGCCACT GTAAACCGGA ACAGGATCTC ATCTGCCAGA 
AGGGTTCAGC ATGTGCCAAG ATACCTCATG TCTTCTCTGC TGGATGAGAC TATCGAGGCT 
GTCGCCACTG GTCTGAGCAC TGCTGTGTAC CAGCGCTCCC TGACTCCTCG TGTCCCTGCA 
AAACGGAAAA GGAAGGCAGG GAGACGGAAA AAAGTGCTTG GAAGAAAGAC GACCCGGTCT 
AGGTCATCTG TGAAAAGGAA GAGTGGGGGT CTGAGAGCCA AGAAACGCCA GCACCGTGTG 
AGGAAGACAA AAGGGAGAAA GATGAAGAAT GAGGTTACAG CTCGTTCTCG AATTGCACGG 
ACACTGGGCC TCCGCAGGCC TGTCCGTGGC ACCAGCATGC CATCAGTGTA CAAACCAGTA 
GACCCCTCCC TGGGGCTAAT GAGGGCAGAT ATCGGAGCAG CTTCTCTGTC ACTGTTTGGA 
GACCCCTACG AGCTGGACCC TTTTGACAGC AATGAAGAAC TGTCTGCAAA TCTGCCTTCC 
CCTCTGAGTG CCAAGAGGAG AGTGCTGTCC CGCTCGGCCT TGCAGTCTCA CCAGCCTGTA 
GCCAGGCCTG TTGCTATGGG GCTCTCCAGA AGGCAGCTCC CAGCTGTGGC CCCAGAGCCC 
AGTGTGGAGG AGGCTCCTGT TCCTGATCTG TTGGGGAGCA TCCTGACTGG CCAGAGCCTC 
CTGATGATGA GCAGCGCTGA TGTTGTCATC CACCGGGATG GCTCTCTTAG CGCAAAGAGG 
ACAGCTCCTG TTTCTCTTCA ACGAAATTCT GTGACTCAAT CCAGAGGAGA AGATGAGTCC 
AGGCGCAGAG ACAGCCTACA GCCTGAGGCA TCACCCTCAG GGAGCCCATC CAATGTCGGA 
CTCACAGGAA GCAGGCCACA GAGCTCAGGG ATAAGCTGTG GGGACAGGTC GGCCCTACGC 
TGTATCCCCG CCTACTCAGT GGGGACACCT GCGAGGCCGG AGTCATCATC AACCCCTGGT 
TCAAGTCAGG CTGGGAAGCT TTCAAATGGG AGCAGGCCTA GCCTGAAGCA GAATAACAGC 
CCCCAGTTTA ATGGCGCGAA CATGCAGGCC TTGCCTCTTC GTTCTGCCTC CTCTAAGATC 
TCAGCCACTC ACTCTAACTC TTCATCTAGA AATATAGTCC TTAGACAGTC CCTGAAAACA 
GCTCCCAGGA GAGCCGACAT CTCACAGTTG CCCAGAATAC CAAAGATCCG TAGGAATGGC 
AGTGGCAGCC GACAAGACCC TGCCCCAGCC CATGGGCAGA GCGTGGAGCT CCCCAGCACC 
TGCATTAGCC GCCTGACAGG AAGGGAGGGT GCTGGACAGC CAGGGCGAGG CCGAGCTGAG 
AATGAGCCCA GAAACAGGGG CCCTCAGGAG CCTGGTTCAC ACACGGGTGG CTCTCGGCCT 
CCTGCCCCCA GCTCCCATGG CAACTTGGCC CCTCTAGGGC CTTCAAGAGG GAAAGGCATT 
GGGTCAACCT TTGAGAGTTT CAGGATCAAC ATCCCCGGGA ATACTGCACA CAGCAGCCAG 
CTGTCCAGCC CTGGCTTCTG TAACACATTC CGGCCAGTGG ATAGCAAAGT GCAGAGGAAG 
GAGAACCCTT CGCCACTCTT CTCCATCAAG AAGCCGAAGC AACTCAAGAG TGAAATCTAT 
GACCCCTTTG ACCCCACTGG CTCTGACTCT AGTCCTCCTA CCAGCAGCCC AGAGAGTATT 
GGCCCAGGCC TTCTGCCATC TGAGATCACG AGAACAATCT CCATCAACAG CCCAAAAGCC 
CCAGCCTTCC AGGCTGTGCG CTGTGTTACC TCCTACAGGG TGGAAAGCAT CTTTGATACT 
GAGGTGGAAC CTGAGCCCCA GCCCCCTGGT GAGCCCATGT CTGGCATGTT CAAGTTCCTG 
GGCAAGGGGC CTGCTGAGGG AGCCTCTGAC TTGGAGCAAG AAAGGCTAGG TGAGGTGGAG 
CATCCAGAAA TCGAGGGTTC CATAGCCCAG ACACACAGGC CATCCCCACA AGACCCCTGG 
GAAGATGAGG ATGGAATGTC CTGTACACCC TTCTTTGGTT CTGAGGAGCG GACAGTGACA 
TGTGTGACTG TCATGGAGCC AGATGTTCCA CAGGTCACCA CACATAGGAT TGTAGAGCTG 
AGAACCCCAT CCCGATCCCG TTCCACCTCT AGCTCCCGAA GCAAGAAGAA AACCAAGAAG 
AAGAGGGCTG CCAGGGAGCA CCAGAGGACA CGCTCTAGCA CTCGCTCTGG CTCCAGGGAC 
AGGACCTCAC GTTCGGTATC GCCCGTGGTC GAGGAGCACA CCAAGAGGCA CAGGGCCAAG 
ACCAAGAGCC GGAGGTCTTC CAGCGACCGT GCCAGCAGCC AGGAGAGAGT GAAGAGGAGG 
AAGACCAGGG ACAGGGACAG GGAGCGGAGA CGGGGCTCCT GGAGCCATGG CAGGTGCCGC 
AGGAAGTCCA GGTCTCACTC AGGCAGTCCT GGCAGTTCTT CCTGTGAGCG CCATGAAAGC 
AGGAAGCGGA AGAGGCGACG CTCAGGCTCC AGGTCTCGGG GCAAGGACTG CTCTTCCCCC 
AGCAACCTAG AGCGGGCCAG GAGACACAAG CACCGGGAAC GGAGCCGGAG CCGAGACAAG 
AAAGACAGTA TGACCTGGTC CCGGGAGAGG AGGAGGTGGA GGTCTAGATC ACCAAGCTTG 
GAGCATAGGC CACGGCGGCC ACGTTCCCGA GAGAAGCGGC CCCGTTCCCC AGAGAAGGCT 
CCAGCTGTGA AGGAAGCTTC CCCAGCCCCC ACTGCTCAGG AGGAGCTGAG GCAAGATGAA 
GACCGCCCGG CTAAACCTTC AGTCTCCGAG GAAGTGAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCCCCAGA AATGCCCCCT GTCCTGGCAG TGCCAGCTGA ATCTGTGCCT 
GAGGACTTGG ACTATGGTGA CTCTGTGGAA GCAGGGCACG TCTTTGAGGA TTTTTCAAGT 
GAAGCCATCT TTATCCAGCT TGATGACATG AGTTCACCTC CTTCCCCTGA GAGTACAGAC 
TCCTCCCCAG AACGAGACTT CCCACCAAAT CCTGTGGTGC CCCCAGCCAT CCAGCCACAG 
GATGCCAGTC TGGCTGCCAT CCAGAGGGAA GTGTCCCTGA TCCACAGTGA AGACATCTCT 
CAGCCCACAC CACAGGCAGA GGGCCCTTCA GACCAGTACC TGCTCAGGCA GGACGCTGTA 
GAGACCATTG CCATGACTCC CAGCACCATG GGTGTGGTAC CCATGGGAAA AGACAGCCCC 
TTGCTGAGTG GGAGAGGGTG TGAAGCAGTC AGGTCCAAGG AGACAGTGGT CCAGGCCCCT 
CTGCTACGGG CCAGGGCCCT TGTAAAAAGA GTCACCTGGA ACCTACAGGA GGCAGAGGAT 
GGTACTCCAG CCCTGGACAG AGTTCCAAGG ACACCACTTC AGAGGCCACA GAGGCCCCGG 
GAAGGAGACT GGGATGCAGA GGACGGGGCC CTCGTAGGAG TTCAGCAGGC ACCATTCTCC 
GAGCTGCCCC CTCCCATCCA CGTGCTCCAG GAGTCTGGGT TACCTGATGC AGACCCCTCT 
CAGCCCCCTG GTGCTTCCAG GGCAGAAGAG GTGCCAGCTG TTGGGACTCT GCACTCAGCA 
GAGGGTATTC TTGCCCAAGT TTACAACCCC AACATGCCAC CACCCCTGGC TCAGCCATCA 
AGCATCCTGC CCTACACCCT CGTCAGTCAG CCCTCGGTCC AGTTAATCCT GCAGGGGACC 
CTTCCCCTGG CTGGCTGTGG CACAGCACAG ACCCTGGCCC CAGTGCCTAA TGTTCCAGCT 
GTAGCCTCAG AGCTGGCTGT TCTAACCAAC AACAGCAGCT CTGAGGAGAG GACCAGCACT 
CCCAAGCCAG CCGCCGAGAA GACCAAGAAG GAAGAGTACT TGAAGAAGCT GCACATGCAG 
GAGCGGGCTG TGGAGGAGGT GAAGCTGGCC ATTAAGCCCT TCTACCAGAA GAGAGAGGTG 
ACCAAGGAGG AATACAAGGA CATCCTGCGG AAAGCTGTGC AGAAGATCTG CCACAGCAAG 
AGCGGCGAGA TCAACCCTGT GAAGGTGGCC AATCTGGTAA AGGCCTATGT GGACAAGTAT 
AGGCACATGC GCAGGCACAA GAAGACTGAA GCTGGGGAGG AGCCACCTGC CCAGGGTGCC 
GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_008854394.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008852616.1 Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X1, mRNA.

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CloneID	ONa36152
Clone ID Related Accession (Same CDS sequence)	XM_008854394.3 , XM_008854394.2
Accession Version	XM_008854394.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	PHD and RING finger domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008356261.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008854394.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGTTTGGA TGAGCTTGTG GCCCACAGCC CTGGGCCAAA TGGACCTCCA 
AGAGTCGTCC CCTCAGAACT GGCCAGTGAA GCTGAAGAAA GTGGCAATGG TCACAGTGGA 
GACTCTGAAG ACTACAGTGG CAGTGAGCAT GAAGACAACA CAGATGGGGA AGAAACAGAG 
GGGTTGTCTG AGGAGGAAGA CCCAGAAGAT AGATCTGGTT CTGAAGATTC AGAAGATGGA 
ATAGAAACAT CAGTGGCAGC AGTGGGTACT CAAGGGAAGC TGGAAACTAG CAGCGCACTC 
AATTCTGATG ATGATGCAGA GAGCTGCCCT ATTTGCCTCA ATGCATTCAG AGACCAGGCT 
GTGGGCACTC CAGAGACCTG TGCCCACTAT TTCTGCTTGG ACTGTATTGT CGAATGGTCT 
AGGAATGCCA ACTCCTGTCC AGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGAGCT 
CGACTTGATG GTAAAATTTT AAAGAAGATT CCAGTGGAAA ACACTAGAGC CCGTGAGGAT 
GAGGAGGCTG AGGAGGAGGA CCCGACCTTC TGTGAGGTGT GTGGTAGGAG TGACCGTGAG 
GACCGGCTCC TGCTCTGTGA TGGCTGTGAT GCTGGGTACC ACATGGAATG TTTAGATCCT 
CCTCTCCAGG AGGTACCTGT GGATGAGTGG TTCTGCCCAG AGTGTGTCAC CCCTGGTGCT 
GCCTCCACTC ATGACACAGC TCCTGTGAGT GATGAGGAGG TCTCACTGTT ACTGGCCGAC 
GTGGTACCCA CCACCAGCAG GCTGCGGCCT CGTGTGGGCA GGACCCGAGC CATCGCCAGG 
ACACGACAGA GTGAGAGAGT GAGGGCCACT GTAAACCGGA ACAGGATCTC ATCTGCCAGA 
AGGGTTCAGC ATGTGCCAAG ATACCTCATG TCTTCTCTGC TGGATGAGAC TATCGAGGCT 
GTCGCCACTG GTCTGAGCAC TGCTGTGTAC CAGCGCTCCC TGACTCCTCG TGTCCCTGCA 
AAACGGAAAA GGAAGGCAGG GAGACGGAAA AAAGTGCTTG GAAGAAAGAC GACCCGGTCT 
AGGTCATCTG TGAAAAGGAA GAGTGGGGGT CTGAGAGCCA AGAAACGCCA GCACCGTGTG 
AGGAAGACAA AAGGGAGAAA GATGAAGAAT GAGGTTACAG CTCGTTCTCG AATTGCACGG 
ACACTGGGCC TCCGCAGGCC TGTCCGTGGC ACCAGCATGC CATCAGTGTA CAAACCAGTA 
GACCCCTCCC TGGGGCTAAT GAGGGCAGAT ATCGGAGCAG CTTCTCTGTC ACTGTTTGGA 
GACCCCTACG AGCTGGACCC TTTTGACAGC AATGAAGAAC TGTCTGCAAA TCTGCCTTCC 
CCTCTGAGTG CCAAGAGGAG AGTGCTGTCC CGCTCGGCCT TGCAGTCTCA CCAGCCTGTA 
GCCAGGCCTG TTGCTATGGG GCTCTCCAGA AGGCAGCTCC CAGCTGTGGC CCCAGAGCCC 
AGTGTGGAGG AGGCTCCTGT TCCTGATCTG TTGGGGAGCA TCCTGACTGG CCAGAGCCTC 
CTGATGATGA GCAGCGCTGA TGTTGTCATC CACCGGGATG GCTCTCTTAG CGCAAAGAGG 
ACAGCTCCTG TTTCTCTTCA ACGAAATTCT GTGACTCAAT CCAGAGGAGA AGATGAGTCC 
AGGCGCAGAG ACAGCCTACA GCCTGAGGCA TCACCCTCAG GGAGCCCATC CAATGTCGGA 
CTCACAGGAA GCAGGCCACA GAGCTCAGGG ATAAGCTGTG GGGACAGGTC GGCCCTACGC 
TGTATCCCCG CCTACTCAGT GGGGACACCT GCGAGGCCGG AGTCATCATC AACCCCTGGT 
TCAAGTCAGG CTGGGAAGCT TTCAAATGGG AGCAGGCCTA GCCTGAAGCA GAATAACAGC 
CCCCAGTTTA ATGGCGCGAA CATGCAGGCC TTGCCTCTTC GTTCTGCCTC CTCTAAGATC 
TCAGCCACTC ACTCTAACTC TTCATCTAGA AATATAGTCC TTAGACAGTC CCTGAAAACA 
GCTCCCAGGA GAGCCGACAT CTCACAGTTG CCCAGAATAC CAAAGATCCG TAGGAATGGC 
AGTGGCAGCC GACAAGACCC TGCCCCAGCC CATGGGCAGA GCGTGGAGCT CCCCAGCACC 
TGCATTAGCC GCCTGACAGG AAGGGAGGGT GCTGGACAGC CAGGGCGAGG CCGAGCTGAG 
AATGAGCCCA GAAACAGGGG CCCTCAGGAG CCTGGTTCAC ACACGGGTGG CTCTCGGCCT 
CCTGCCCCCA GCTCCCATGG CAACTTGGCC CCTCTAGGGC CTTCAAGAGG GAAAGGCATT 
GGGTCAACCT TTGAGAGTTT CAGGATCAAC ATCCCCGGGA ATACTGCACA CAGCAGCCAG 
CTGTCCAGCC CTGGCTTCTG TAACACATTC CGGCCAGTGG ATAGCAAAGT GCAGAGGAAG 
GAGAACCCTT CGCCACTCTT CTCCATCAAG AAGCCGAAGC AACTCAAGAG TGAAATCTAT 
GACCCCTTTG ACCCCACTGG CTCTGACTCT AGTCCTCCTA CCAGCAGCCC AGAGAGTATT 
GGCCCAGGCC TTCTGCCATC TGAGATCACG AGAACAATCT CCATCAACAG CCCAAAAGCC 
CCAGCCTTCC AGGCTGTGCG CTGTGTTACC TCCTACAGGG TGGAAAGCAT CTTTGATACT 
GAGGTGGAAC CTGAGCCCCA GCCCCCTGGT GAGCCCATGT CTGGCATGTT CAAGTTCCTG 
GGCAAGGGGC CTGCTGAGGG AGCCTCTGAC TTGGAGCAAG AAAGGCTAGG TGAGGTGGAG 
CATCCAGAAA TCGAGGGTTC CATAGCCCAG ACACACAGGC CATCCCCACA AGACCCCTGG 
GAAGATGAGG ATGGAATGTC CTGTACACCC TTCTTTGGTT CTGAGGAGCG GACAGTGACA 
TGTGTGACTG TCATGGAGCC AGATGTTCCA CAGGTCACCA CACATAGGAT TGTAGAGCTG 
AGAACCCCAT CCCGATCCCG TTCCACCTCT AGCTCCCGAA GCAAGAAGAA AACCAAGAAG 
AAGAGGGCTG CCAGGGAGCA CCAGAGGACA CGCTCTAGCA CTCGCTCTGG CTCCAGGGAC 
AGGACCTCAC GTTCGGTATC GCCCGTGGTC GAGGAGCACA CCAAGAGGCA CAGGGCCAAG 
ACCAAGAGCC GGAGGTCTTC CAGCGACCGT GCCAGCAGCC AGGAGAGAGT GAAGAGGAGG 
AAGACCAGGG ACAGGGACAG GGAGCGGAGA CGGGGCTCCT GGAGCCATGG CAGGTGCCGC 
AGGAAGTCCA GGTCTCACTC AGGCAGTCCT GGCAGTTCTT CCTGTGAGCG CCATGAAAGC 
AGGAAGCGGA AGAGGCGACG CTCAGGCTCC AGGTCTCGGG GCAAGGACTG CTCTTCCCCC 
AGCAACCTAG AGCGGGCCAG GAGACACAAG CACCGGGAAC GGAGCCGGAG CCGAGACAAG 
AAAGACAGTA TGACCTGGTC CCGGGAGAGG AGGAGGTGGA GGTCTAGATC ACCAAGCTTG 
GAGCATAGGC CACGGCGGCC ACGTTCCCGA GAGAAGCGGC CCCGTTCCCC AGAGAAGGCT 
CCAGCTGTGA AGGAAGCTTC CCCAGCCCCC ACTGCTCAGG AGGAGCTGAG GCAAGATGAA 
GACCGCCCGG CTAAACCTTC AGTCTCCGAG GAAGTGAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCCCCAGA AATGCCCCCT GTCCTGGCAG TGCCAGCTGA ATCTGTGCCT 
GAGGACTTGG ACTATGGTGA CTCTGTGGAA GCAGGGCACG TCTTTGAGGA TTTTTCAAGT 
GAAGCCATCT TTATCCAGCT TGATGACATG AGTTCACCTC CTTCCCCTGA GAGTACAGAC 
TCCTCCCCAG AACGAGACTT CCCACCAAAT CCTGTGGTGC CCCCAGCCAT CCAGCCACAG 
GATGCCAGTC TGGCTGCCAT CCAGAGGGAA GTGTCCCTGA TCCACAGTGA AGACATCTCT 
CAGCCCACAC CACAGGCAGA GGGCCCTTCA GACCAGTACC TGCTCAGGCA GGACGCTGTA 
GAGACCATTG CCATGACTCC CAGCACCATG GGTGTGGTAC CCATGGGAAA AGACAGCCCC 
TTGCTGAGTG GGAGAGGGTG TGAAGCAGTC AGGTCCAAGG AGACAGTGGT CCAGGCCCCT 
CTGCTACGGG CCAGGGCCCT TGTAAAAAGA GTCACCTGGA ACCTACAGGA GGCAGAGGAT 
GGTACTCCAG CCCTGGACAG AGTTCCAAGG ACACCACTTC AGAGGCCACA GAGGCCCCGG 
GAAGGAGACT GGGATGCAGA GGACGGGGCC CTCGTAGGAG TTCAGCAGGC ACCATTCTCC 
GAGCTGCCCC CTCCCATCCA CGTGCTCCAG GAGTCTGGGT TACCTGATGC AGACCCCTCT 
CAGCCCCCTG GTGCTTCCAG GGCAGAAGAG GTGCCAGCTG TTGGGACTCT GCACTCAGCA 
GAGGGTATTC TTGCCCAAGT TTACAACCCC AACATGCCAC CACCCCTGGC TCAGCCATCA 
AGCATCCTGC CCTACACCCT CGTCAGTCAG CCCTCGGTCC AGTTAATCCT GCAGGGGACC 
CTTCCCCTGG CTGGCTGTGG CACAGCACAG ACCCTGGCCC CAGTGCCTAA TGTTCCAGCT 
GTAGCCTCAG AGCTGGCTGT TCTAACCAAC AACAGCAGCT CTGAGGAGAG GACCAGCACT 
CCCAAGCCAG CCGCCGAGAA GACCAAGAAG GAAGAGTACT TGAAGAAGCT GCACATGCAG 
GAGCGGGCTG TGGAGGAGGT GAAGCTGGCC ATTAAGCCCT TCTACCAGAA GAGAGAGGTG 
ACCAAGGAGG AATACAAGGA CATCCTGCGG AAAGCTGTGC AGAAGATCTG CCACAGCAAG 
AGCGGCGAGA TCAACCCTGT GAAGGTGGCC AATCTGGTAA AGGCCTATGT GGACAAGTAT 
AGGCACATGC GCAGGCACAA GAAGACTGAA GCTGGGGAGG AGCCACCTGC CCAGGGTGCC 
GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008852616.1
CDS	116..5164
Translation	MDDDSLDELVAHSPGPNGPPRVVPSELASEAEESGNGHSGDSEDYSGSEHEDNTDGEETEGLSEEEDPEDRSGSEDSEDGIETSVAAVGTQGKLETSSALNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIVEWSRNANSCPVDRTVFKCICIRARLDGKILKKIPVENTRAREDEEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECVTPGAASTHDTAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRSLTPRVPAKRKRKAGRRKKVLGRKTTRSRSSVKRKSGGLRAKKRQHRVRKTKGRKMKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEELSANLPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILTGQSLLMMSSADVVIHRDGSLSAKRTAPVSLQRNSVTQSRGEDESRRRDSLQPEASPSGSPSNVGLTGSRPQSSGISCGDRSALRCIPAYSVGTPARPESSSTPGSSQAGKLSNGSRPSLKQNNSPQFNGANMQALPLRSASSKISATHSNSSSRNIVLRQSLKTAPRRADISQLPRIPKIRRNGSGSRQDPAPAHGQSVELPSTCISRLTGREGAGQPGRGRAENEPRNRGPQEPGSHTGGSRPPAPSSHGNLAPLGPSRGKGIGSTFESFRINIPGNTAHSSQLSSPGFCNTFRPVDSKVQRKENPSPLFSIKKPKQLKSEIYDPFDPTGSDSSPPTSSPESIGPGLLPSEITRTISINSPKAPAFQAVRCVTSYRVESIFDTEVEPEPQPPGEPMSGMFKFLGKGPAEGASDLEQERLGEVEHPEIEGSIAQTHRPSPQDPWEDEDGMSCTPFFGSEERTVTCVTVMEPDVPQVTTHRIVELRTPSRSRSTSSSRSKKKTKKKRAAREHQRTRSSTRSGSRDRTSRSVSPVVEEHTKRHRAKTKSRRSSSDRASSQERVKRRKTRDRDRERRRGSWSHGRCRRKSRSHSGSPGSSSCERHESRKRKRRRSGSRSRGKDCSSPSNLERARRHKHRERSRSRDKKDSMTWSRERRRWRSRSPSLEHRPRRPRSREKRPRSPEKAPAVKEASPAPTAQEELRQDEDRPAKPSVSEEVSVLPEVVVADLNPPEMPPVLAVPAESVPEDLDYGDSVEAGHVFEDFSSEAIFIQLDDMSSPPSPESTDSSPERDFPPNPVVPPAIQPQDASLAAIQREVSLIHSEDISQPTPQAEGPSDQYLLRQDAVETIAMTPSTMGVVPMGKDSPLLSGRGCEAVRSKETVVQAPLLRARALVKRVTWNLQEAEDGTPALDRVPRTPLQRPQRPREGDWDAEDGALVGVQQAPFSELPPPIHVLQESGLPDADPSQPPGASRAEEVPAVGTLHSAEGILAQVYNPNMPPPLAQPSSILPYTLVSQPSVQLILQGTLPLAGCGTAQTLAPVPNVPAVASELAVLTNNSSSEERTSTPKPAAEKTKKEEYLKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEAGEEPPAQGAET

Target ORF information:

RefSeq Version	XM_008854394.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008854394.2

ORF Insert Sequence:

ATGGATGACG ACAGTTTGGA TGAGCTTGTG GCCCACAGCC CTGGGCCAAA TGGACCTCCA 
AGAGTCGTCC CCTCAGAACT GGCCAGTGAA GCTGAAGAAA GTGGCAATGG TCACAGTGGA 
GACTCTGAAG ACTACAGTGG CAGTGAGCAT GAAGACAACA CAGATGGGGA AGAAACAGAG 
GGGTTGTCTG AGGAGGAAGA CCCAGAAGAT AGATCTGGTT CTGAAGATTC AGAAGATGGA 
ATAGAAACAT CAGTGGCAGC AGTGGGTACT CAAGGGAAGC TGGAAACTAG CAGCGCACTC 
AATTCTGATG ATGATGCAGA GAGCTGCCCT ATTTGCCTCA ATGCATTCAG AGACCAGGCT 
GTGGGCACTC CAGAGACCTG TGCCCACTAT TTCTGCTTGG ACTGTATTGT CGAATGGTCT 
AGGAATGCCA ACTCCTGTCC AGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGAGCT 
CGACTTGATG GTAAAATTTT AAAGAAGATT CCAGTGGAAA ACACTAGAGC CCGTGAGGAT 
GAGGAGGCTG AGGAGGAGGA CCCGACCTTC TGTGAGGTGT GTGGTAGGAG TGACCGTGAG 
GACCGGCTCC TGCTCTGTGA TGGCTGTGAT GCTGGGTACC ACATGGAATG TTTAGATCCT 
CCTCTCCAGG AGGTACCTGT GGATGAGTGG TTCTGCCCAG AGTGTGTCAC CCCTGGTGCT 
GCCTCCACTC ATGACACAGC TCCTGTGAGT GATGAGGAGG TCTCACTGTT ACTGGCCGAC 
GTGGTACCCA CCACCAGCAG GCTGCGGCCT CGTGTGGGCA GGACCCGAGC CATCGCCAGG 
ACACGACAGA GTGAGAGAGT GAGGGCCACT GTAAACCGGA ACAGGATCTC ATCTGCCAGA 
AGGGTTCAGC ATGTGCCAAG ATACCTCATG TCTTCTCTGC TGGATGAGAC TATCGAGGCT 
GTCGCCACTG GTCTGAGCAC TGCTGTGTAC CAGCGCTCCC TGACTCCTCG TGTCCCTGCA 
AAACGGAAAA GGAAGGCAGG GAGACGGAAA AAAGTGCTTG GAAGAAAGAC GACCCGGTCT 
AGGTCATCTG TGAAAAGGAA GAGTGGGGGT CTGAGAGCCA AGAAACGCCA GCACCGTGTG 
AGGAAGACAA AAGGGAGAAA GATGAAGAAT GAGGTTACAG CTCGTTCTCG AATTGCACGG 
ACACTGGGCC TCCGCAGGCC TGTCCGTGGC ACCAGCATGC CATCAGTGTA CAAACCAGTA 
GACCCCTCCC TGGGGCTAAT GAGGGCAGAT ATCGGAGCAG CTTCTCTGTC ACTGTTTGGA 
GACCCCTACG AGCTGGACCC TTTTGACAGC AATGAAGAAC TGTCTGCAAA TCTGCCTTCC 
CCTCTGAGTG CCAAGAGGAG AGTGCTGTCC CGCTCGGCCT TGCAGTCTCA CCAGCCTGTA 
GCCAGGCCTG TTGCTATGGG GCTCTCCAGA AGGCAGCTCC CAGCTGTGGC CCCAGAGCCC 
AGTGTGGAGG AGGCTCCTGT TCCTGATCTG TTGGGGAGCA TCCTGACTGG CCAGAGCCTC 
CTGATGATGA GCAGCGCTGA TGTTGTCATC CACCGGGATG GCTCTCTTAG CGCAAAGAGG 
ACAGCTCCTG TTTCTCTTCA ACGAAATTCT GTGACTCAAT CCAGAGGAGA AGATGAGTCC 
AGGCGCAGAG ACAGCCTACA GCCTGAGGCA TCACCCTCAG GGAGCCCATC CAATGTCGGA 
CTCACAGGAA GCAGGCCACA GAGCTCAGGG ATAAGCTGTG GGGACAGGTC GGCCCTACGC 
TGTATCCCCG CCTACTCAGT GGGGACACCT GCGAGGCCGG AGTCATCATC AACCCCTGGT 
TCAAGTCAGG CTGGGAAGCT TTCAAATGGG AGCAGGCCTA GCCTGAAGCA GAATAACAGC 
CCCCAGTTTA ATGGCGCGAA CATGCAGGCC TTGCCTCTTC GTTCTGCCTC CTCTAAGATC 
TCAGCCACTC ACTCTAACTC TTCATCTAGA AATATAGTCC TTAGACAGTC CCTGAAAACA 
GCTCCCAGGA GAGCCGACAT CTCACAGTTG CCCAGAATAC CAAAGATCCG TAGGAATGGC 
AGTGGCAGCC GACAAGACCC TGCCCCAGCC CATGGGCAGA GCGTGGAGCT CCCCAGCACC 
TGCATTAGCC GCCTGACAGG AAGGGAGGGT GCTGGACAGC CAGGGCGAGG CCGAGCTGAG 
AATGAGCCCA GAAACAGGGG CCCTCAGGAG CCTGGTTCAC ACACGGGTGG CTCTCGGCCT 
CCTGCCCCCA GCTCCCATGG CAACTTGGCC CCTCTAGGGC CTTCAAGAGG GAAAGGCATT 
GGGTCAACCT TTGAGAGTTT CAGGATCAAC ATCCCCGGGA ATACTGCACA CAGCAGCCAG 
CTGTCCAGCC CTGGCTTCTG TAACACATTC CGGCCAGTGG ATAGCAAAGT GCAGAGGAAG 
GAGAACCCTT CGCCACTCTT CTCCATCAAG AAGCCGAAGC AACTCAAGAG TGAAATCTAT 
GACCCCTTTG ACCCCACTGG CTCTGACTCT AGTCCTCCTA CCAGCAGCCC AGAGAGTATT 
GGCCCAGGCC TTCTGCCATC TGAGATCACG AGAACAATCT CCATCAACAG CCCAAAAGCC 
CCAGCCTTCC AGGCTGTGCG CTGTGTTACC TCCTACAGGG TGGAAAGCAT CTTTGATACT 
GAGGTGGAAC CTGAGCCCCA GCCCCCTGGT GAGCCCATGT CTGGCATGTT CAAGTTCCTG 
GGCAAGGGGC CTGCTGAGGG AGCCTCTGAC TTGGAGCAAG AAAGGCTAGG TGAGGTGGAG 
CATCCAGAAA TCGAGGGTTC CATAGCCCAG ACACACAGGC CATCCCCACA AGACCCCTGG 
GAAGATGAGG ATGGAATGTC CTGTACACCC TTCTTTGGTT CTGAGGAGCG GACAGTGACA 
TGTGTGACTG TCATGGAGCC AGATGTTCCA CAGGTCACCA CACATAGGAT TGTAGAGCTG 
AGAACCCCAT CCCGATCCCG TTCCACCTCT AGCTCCCGAA GCAAGAAGAA AACCAAGAAG 
AAGAGGGCTG CCAGGGAGCA CCAGAGGACA CGCTCTAGCA CTCGCTCTGG CTCCAGGGAC 
AGGACCTCAC GTTCGGTATC GCCCGTGGTC GAGGAGCACA CCAAGAGGCA CAGGGCCAAG 
ACCAAGAGCC GGAGGTCTTC CAGCGACCGT GCCAGCAGCC AGGAGAGAGT GAAGAGGAGG 
AAGACCAGGG ACAGGGACAG GGAGCGGAGA CGGGGCTCCT GGAGCCATGG CAGGTGCCGC 
AGGAAGTCCA GGTCTCACTC AGGCAGTCCT GGCAGTTCTT CCTGTGAGCG CCATGAAAGC 
AGGAAGCGGA AGAGGCGACG CTCAGGCTCC AGGTCTCGGG GCAAGGACTG CTCTTCCCCC 
AGCAACCTAG AGCGGGCCAG GAGACACAAG CACCGGGAAC GGAGCCGGAG CCGAGACAAG 
AAAGACAGTA TGACCTGGTC CCGGGAGAGG AGGAGGTGGA GGTCTAGATC ACCAAGCTTG 
GAGCATAGGC CACGGCGGCC ACGTTCCCGA GAGAAGCGGC CCCGTTCCCC AGAGAAGGCT 
CCAGCTGTGA AGGAAGCTTC CCCAGCCCCC ACTGCTCAGG AGGAGCTGAG GCAAGATGAA 
GACCGCCCGG CTAAACCTTC AGTCTCCGAG GAAGTGAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCCCCAGA AATGCCCCCT GTCCTGGCAG TGCCAGCTGA ATCTGTGCCT 
GAGGACTTGG ACTATGGTGA CTCTGTGGAA GCAGGGCACG TCTTTGAGGA TTTTTCAAGT 
GAAGCCATCT TTATCCAGCT TGATGACATG AGTTCACCTC CTTCCCCTGA GAGTACAGAC 
TCCTCCCCAG AACGAGACTT CCCACCAAAT CCTGTGGTGC CCCCAGCCAT CCAGCCACAG 
GATGCCAGTC TGGCTGCCAT CCAGAGGGAA GTGTCCCTGA TCCACAGTGA AGACATCTCT 
CAGCCCACAC CACAGGCAGA GGGCCCTTCA GACCAGTACC TGCTCAGGCA GGACGCTGTA 
GAGACCATTG CCATGACTCC CAGCACCATG GGTGTGGTAC CCATGGGAAA AGACAGCCCC 
TTGCTGAGTG GGAGAGGGTG TGAAGCAGTC AGGTCCAAGG AGACAGTGGT CCAGGCCCCT 
CTGCTACGGG CCAGGGCCCT TGTAAAAAGA GTCACCTGGA ACCTACAGGA GGCAGAGGAT 
GGTACTCCAG CCCTGGACAG AGTTCCAAGG ACACCACTTC AGAGGCCACA GAGGCCCCGG 
GAAGGAGACT GGGATGCAGA GGACGGGGCC CTCGTAGGAG TTCAGCAGGC ACCATTCTCC 
GAGCTGCCCC CTCCCATCCA CGTGCTCCAG GAGTCTGGGT TACCTGATGC AGACCCCTCT 
CAGCCCCCTG GTGCTTCCAG GGCAGAAGAG GTGCCAGCTG TTGGGACTCT GCACTCAGCA 
GAGGGTATTC TTGCCCAAGT TTACAACCCC AACATGCCAC CACCCCTGGC TCAGCCATCA 
AGCATCCTGC CCTACACCCT CGTCAGTCAG CCCTCGGTCC AGTTAATCCT GCAGGGGACC 
CTTCCCCTGG CTGGCTGTGG CACAGCACAG ACCCTGGCCC CAGTGCCTAA TGTTCCAGCT 
GTAGCCTCAG AGCTGGCTGT TCTAACCAAC AACAGCAGCT CTGAGGAGAG GACCAGCACT 
CCCAAGCCAG CCGCCGAGAA GACCAAGAAG GAAGAGTACT TGAAGAAGCT GCACATGCAG 
GAGCGGGCTG TGGAGGAGGT GAAGCTGGCC ATTAAGCCCT TCTACCAGAA GAGAGAGGTG 
ACCAAGGAGG AATACAAGGA CATCCTGCGG AAAGCTGTGC AGAAGATCTG CCACAGCAAG 
AGCGGCGAGA TCAACCCTGT GAAGGTGGCC AATCTGGTAA AGGCCTATGT GGACAAGTAT 
AGGCACATGC GCAGGCACAA GAAGACTGAA GCTGGGGAGG AGCCACCTGC CCAGGGTGCC 
GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_008854395.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008852617.1 Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X2, mRNA.

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CloneID	ONa36153
Clone ID Related Accession (Same CDS sequence)	XM_008854395.2 , XM_008854395.3
Accession Version	XM_008854395.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	PHD and RING finger domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008356261.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008854395.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGTTTGGA TGAGCTTGTG GCCCACAGCC CTGGGCCAAA TGGACCTCCA 
AGAGTCGTCC CCTCAGAACT GGCCAGTGAA GCTGAAAGTG GCAATGGTCA CAGTGGAGAC 
TCTGAAGACT ACAGTGGCAG TGAGCATGAA GACAACACAG ATGGGGAAGA AACAGAGGGG 
TTGTCTGAGG AGGAAGACCC AGAAGATAGA TCTGGTTCTG AAGATTCAGA AGATGGAATA 
GAAACATCAG TGGCAGCAGT GGGTACTCAA GGGAAGCTGG AAACTAGCAG CGCACTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCTATT TGCCTCAATG CATTCAGAGA CCAGGCTGTG 
GGCACTCCAG AGACCTGTGC CCACTATTTC TGCTTGGACT GTATTGTCGA ATGGTCTAGG 
AATGCCAACT CCTGTCCAGT TGATCGAACA GTATTTAAAT GTATTTGTAT TCGAGCTCGA 
CTTGATGGTA AAATTTTAAA GAAGATTCCA GTGGAAAACA CTAGAGCCCG TGAGGATGAG 
GAGGCTGAGG AGGAGGACCC GACCTTCTGT GAGGTGTGTG GTAGGAGTGA CCGTGAGGAC 
CGGCTCCTGC TCTGTGATGG CTGTGATGCT GGGTACCACA TGGAATGTTT AGATCCTCCT 
CTCCAGGAGG TACCTGTGGA TGAGTGGTTC TGCCCAGAGT GTGTCACCCC TGGTGCTGCC 
TCCACTCATG ACACAGCTCC TGTGAGTGAT GAGGAGGTCT CACTGTTACT GGCCGACGTG 
GTACCCACCA CCAGCAGGCT GCGGCCTCGT GTGGGCAGGA CCCGAGCCAT CGCCAGGACA 
CGACAGAGTG AGAGAGTGAG GGCCACTGTA AACCGGAACA GGATCTCATC TGCCAGAAGG 
GTTCAGCATG TGCCAAGATA CCTCATGTCT TCTCTGCTGG ATGAGACTAT CGAGGCTGTC 
GCCACTGGTC TGAGCACTGC TGTGTACCAG CGCTCCCTGA CTCCTCGTGT CCCTGCAAAA 
CGGAAAAGGA AGGCAGGGAG ACGGAAAAAA GTGCTTGGAA GAAAGACGAC CCGGTCTAGG 
TCATCTGTGA AAAGGAAGAG TGGGGGTCTG AGAGCCAAGA AACGCCAGCA CCGTGTGAGG 
AAGACAAAAG GGAGAAAGAT GAAGAATGAG GTTACAGCTC GTTCTCGAAT TGCACGGACA 
CTGGGCCTCC GCAGGCCTGT CCGTGGCACC AGCATGCCAT CAGTGTACAA ACCAGTAGAC 
CCCTCCCTGG GGCTAATGAG GGCAGATATC GGAGCAGCTT CTCTGTCACT GTTTGGAGAC 
CCCTACGAGC TGGACCCTTT TGACAGCAAT GAAGAACTGT CTGCAAATCT GCCTTCCCCT 
CTGAGTGCCA AGAGGAGAGT GCTGTCCCGC TCGGCCTTGC AGTCTCACCA GCCTGTAGCC 
AGGCCTGTTG CTATGGGGCT CTCCAGAAGG CAGCTCCCAG CTGTGGCCCC AGAGCCCAGT 
GTGGAGGAGG CTCCTGTTCC TGATCTGTTG GGGAGCATCC TGACTGGCCA GAGCCTCCTG 
ATGATGAGCA GCGCTGATGT TGTCATCCAC CGGGATGGCT CTCTTAGCGC AAAGAGGACA 
GCTCCTGTTT CTCTTCAACG AAATTCTGTG ACTCAATCCA GAGGAGAAGA TGAGTCCAGG 
CGCAGAGACA GCCTACAGCC TGAGGCATCA CCCTCAGGGA GCCCATCCAA TGTCGGACTC 
ACAGGAAGCA GGCCACAGAG CTCAGGGATA AGCTGTGGGG ACAGGTCGGC CCTACGCTGT 
ATCCCCGCCT ACTCAGTGGG GACACCTGCG AGGCCGGAGT CATCATCAAC CCCTGGTTCA 
AGTCAGGCTG GGAAGCTTTC AAATGGGAGC AGGCCTAGCC TGAAGCAGAA TAACAGCCCC 
CAGTTTAATG GCGCGAACAT GCAGGCCTTG CCTCTTCGTT CTGCCTCCTC TAAGATCTCA 
GCCACTCACT CTAACTCTTC ATCTAGAAAT ATAGTCCTTA GACAGTCCCT GAAAACAGCT 
CCCAGGAGAG CCGACATCTC ACAGTTGCCC AGAATACCAA AGATCCGTAG GAATGGCAGT 
GGCAGCCGAC AAGACCCTGC CCCAGCCCAT GGGCAGAGCG TGGAGCTCCC CAGCACCTGC 
ATTAGCCGCC TGACAGGAAG GGAGGGTGCT GGACAGCCAG GGCGAGGCCG AGCTGAGAAT 
GAGCCCAGAA ACAGGGGCCC TCAGGAGCCT GGTTCACACA CGGGTGGCTC TCGGCCTCCT 
GCCCCCAGCT CCCATGGCAA CTTGGCCCCT CTAGGGCCTT CAAGAGGGAA AGGCATTGGG 
TCAACCTTTG AGAGTTTCAG GATCAACATC CCCGGGAATA CTGCACACAG CAGCCAGCTG 
TCCAGCCCTG GCTTCTGTAA CACATTCCGG CCAGTGGATA GCAAAGTGCA GAGGAAGGAG 
AACCCTTCGC CACTCTTCTC CATCAAGAAG CCGAAGCAAC TCAAGAGTGA AATCTATGAC 
CCCTTTGACC CCACTGGCTC TGACTCTAGT CCTCCTACCA GCAGCCCAGA GAGTATTGGC 
CCAGGCCTTC TGCCATCTGA GATCACGAGA ACAATCTCCA TCAACAGCCC AAAAGCCCCA 
GCCTTCCAGG CTGTGCGCTG TGTTACCTCC TACAGGGTGG AAAGCATCTT TGATACTGAG 
GTGGAACCTG AGCCCCAGCC CCCTGGTGAG CCCATGTCTG GCATGTTCAA GTTCCTGGGC 
AAGGGGCCTG CTGAGGGAGC CTCTGACTTG GAGCAAGAAA GGCTAGGTGA GGTGGAGCAT 
CCAGAAATCG AGGGTTCCAT AGCCCAGACA CACAGGCCAT CCCCACAAGA CCCCTGGGAA 
GATGAGGATG GAATGTCCTG TACACCCTTC TTTGGTTCTG AGGAGCGGAC AGTGACATGT 
GTGACTGTCA TGGAGCCAGA TGTTCCACAG GTCACCACAC ATAGGATTGT AGAGCTGAGA 
ACCCCATCCC GATCCCGTTC CACCTCTAGC TCCCGAAGCA AGAAGAAAAC CAAGAAGAAG 
AGGGCTGCCA GGGAGCACCA GAGGACACGC TCTAGCACTC GCTCTGGCTC CAGGGACAGG 
ACCTCACGTT CGGTATCGCC CGTGGTCGAG GAGCACACCA AGAGGCACAG GGCCAAGACC 
AAGAGCCGGA GGTCTTCCAG CGACCGTGCC AGCAGCCAGG AGAGAGTGAA GAGGAGGAAG 
ACCAGGGACA GGGACAGGGA GCGGAGACGG GGCTCCTGGA GCCATGGCAG GTGCCGCAGG 
AAGTCCAGGT CTCACTCAGG CAGTCCTGGC AGTTCTTCCT GTGAGCGCCA TGAAAGCAGG 
AAGCGGAAGA GGCGACGCTC AGGCTCCAGG TCTCGGGGCA AGGACTGCTC TTCCCCCAGC 
AACCTAGAGC GGGCCAGGAG ACACAAGCAC CGGGAACGGA GCCGGAGCCG AGACAAGAAA 
GACAGTATGA CCTGGTCCCG GGAGAGGAGG AGGTGGAGGT CTAGATCACC AAGCTTGGAG 
CATAGGCCAC GGCGGCCACG TTCCCGAGAG AAGCGGCCCC GTTCCCCAGA GAAGGCTCCA 
GCTGTGAAGG AAGCTTCCCC AGCCCCCACT GCTCAGGAGG AGCTGAGGCA AGATGAAGAC 
CGCCCGGCTA AACCTTCAGT CTCCGAGGAA GTGAGTGTCC TGCCAGAGGT GGTTGTGGCT 
GACCTGAACC CCCCAGAAAT GCCCCCTGTC CTGGCAGTGC CAGCTGAATC TGTGCCTGAG 
GACTTGGACT ATGGTGACTC TGTGGAAGCA GGGCACGTCT TTGAGGATTT TTCAAGTGAA 
GCCATCTTTA TCCAGCTTGA TGACATGAGT TCACCTCCTT CCCCTGAGAG TACAGACTCC 
TCCCCAGAAC GAGACTTCCC ACCAAATCCT GTGGTGCCCC CAGCCATCCA GCCACAGGAT 
GCCAGTCTGG CTGCCATCCA GAGGGAAGTG TCCCTGATCC ACAGTGAAGA CATCTCTCAG 
CCCACACCAC AGGCAGAGGG CCCTTCAGAC CAGTACCTGC TCAGGCAGGA CGCTGTAGAG 
ACCATTGCCA TGACTCCCAG CACCATGGGT GTGGTACCCA TGGGAAAAGA CAGCCCCTTG 
CTGAGTGGGA GAGGGTGTGA AGCAGTCAGG TCCAAGGAGA CAGTGGTCCA GGCCCCTCTG 
CTACGGGCCA GGGCCCTTGT AAAAAGAGTC ACCTGGAACC TACAGGAGGC AGAGGATGGT 
ACTCCAGCCC TGGACAGAGT TCCAAGGACA CCACTTCAGA GGCCACAGAG GCCCCGGGAA 
GGAGACTGGG ATGCAGAGGA CGGGGCCCTC GTAGGAGTTC AGCAGGCACC ATTCTCCGAG 
CTGCCCCCTC CCATCCACGT GCTCCAGGAG TCTGGGTTAC CTGATGCAGA CCCCTCTCAG 
CCCCCTGGTG CTTCCAGGGC AGAAGAGGTG CCAGCTGTTG GGACTCTGCA CTCAGCAGAG 
GGTATTCTTG CCCAAGTTTA CAACCCCAAC ATGCCACCAC CCCTGGCTCA GCCATCAAGC 
ATCCTGCCCT ACACCCTCGT CAGTCAGCCC TCGGTCCAGT TAATCCTGCA GGGGACCCTT 
CCCCTGGCTG GCTGTGGCAC AGCACAGACC CTGGCCCCAG TGCCTAATGT TCCAGCTGTA 
GCCTCAGAGC TGGCTGTTCT AACCAACAAC AGCAGCTCTG AGGAGAGGAC CAGCACTCCC 
AAGCCAGCCG CCGAGAAGAC CAAGAAGGAA GAGTACTTGA AGAAGCTGCA CATGCAGGAG 
CGGGCTGTGG AGGAGGTGAA GCTGGCCATT AAGCCCTTCT ACCAGAAGAG AGAGGTGACC 
AAGGAGGAAT ACAAGGACAT CCTGCGGAAA GCTGTGCAGA AGATCTGCCA CAGCAAGAGC 
GGCGAGATCA ACCCTGTGAA GGTGGCCAAT CTGGTAAAGG CCTATGTGGA CAAGTATAGG 
CACATGCGCA GGCACAAGAA GACTGAAGCT GGGGAGGAGC CACCTGCCCA GGGTGCCGAG 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008852617.1
CDS	116..5161
Translation	MDDDSLDELVAHSPGPNGPPRVVPSELASEAESGNGHSGDSEDYSGSEHEDNTDGEETEGLSEEEDPEDRSGSEDSEDGIETSVAAVGTQGKLETSSALNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIVEWSRNANSCPVDRTVFKCICIRARLDGKILKKIPVENTRAREDEEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECVTPGAASTHDTAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRSLTPRVPAKRKRKAGRRKKVLGRKTTRSRSSVKRKSGGLRAKKRQHRVRKTKGRKMKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEELSANLPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILTGQSLLMMSSADVVIHRDGSLSAKRTAPVSLQRNSVTQSRGEDESRRRDSLQPEASPSGSPSNVGLTGSRPQSSGISCGDRSALRCIPAYSVGTPARPESSSTPGSSQAGKLSNGSRPSLKQNNSPQFNGANMQALPLRSASSKISATHSNSSSRNIVLRQSLKTAPRRADISQLPRIPKIRRNGSGSRQDPAPAHGQSVELPSTCISRLTGREGAGQPGRGRAENEPRNRGPQEPGSHTGGSRPPAPSSHGNLAPLGPSRGKGIGSTFESFRINIPGNTAHSSQLSSPGFCNTFRPVDSKVQRKENPSPLFSIKKPKQLKSEIYDPFDPTGSDSSPPTSSPESIGPGLLPSEITRTISINSPKAPAFQAVRCVTSYRVESIFDTEVEPEPQPPGEPMSGMFKFLGKGPAEGASDLEQERLGEVEHPEIEGSIAQTHRPSPQDPWEDEDGMSCTPFFGSEERTVTCVTVMEPDVPQVTTHRIVELRTPSRSRSTSSSRSKKKTKKKRAAREHQRTRSSTRSGSRDRTSRSVSPVVEEHTKRHRAKTKSRRSSSDRASSQERVKRRKTRDRDRERRRGSWSHGRCRRKSRSHSGSPGSSSCERHESRKRKRRRSGSRSRGKDCSSPSNLERARRHKHRERSRSRDKKDSMTWSRERRRWRSRSPSLEHRPRRPRSREKRPRSPEKAPAVKEASPAPTAQEELRQDEDRPAKPSVSEEVSVLPEVVVADLNPPEMPPVLAVPAESVPEDLDYGDSVEAGHVFEDFSSEAIFIQLDDMSSPPSPESTDSSPERDFPPNPVVPPAIQPQDASLAAIQREVSLIHSEDISQPTPQAEGPSDQYLLRQDAVETIAMTPSTMGVVPMGKDSPLLSGRGCEAVRSKETVVQAPLLRARALVKRVTWNLQEAEDGTPALDRVPRTPLQRPQRPREGDWDAEDGALVGVQQAPFSELPPPIHVLQESGLPDADPSQPPGASRAEEVPAVGTLHSAEGILAQVYNPNMPPPLAQPSSILPYTLVSQPSVQLILQGTLPLAGCGTAQTLAPVPNVPAVASELAVLTNNSSSEERTSTPKPAAEKTKKEEYLKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEAGEEPPAQGAET

Target ORF information:

RefSeq Version	XM_008854395.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008854395.2

ORF Insert Sequence:

ATGGATGACG ACAGTTTGGA TGAGCTTGTG GCCCACAGCC CTGGGCCAAA TGGACCTCCA 
AGAGTCGTCC CCTCAGAACT GGCCAGTGAA GCTGAAAGTG GCAATGGTCA CAGTGGAGAC 
TCTGAAGACT ACAGTGGCAG TGAGCATGAA GACAACACAG ATGGGGAAGA AACAGAGGGG 
TTGTCTGAGG AGGAAGACCC AGAAGATAGA TCTGGTTCTG AAGATTCAGA AGATGGAATA 
GAAACATCAG TGGCAGCAGT GGGTACTCAA GGGAAGCTGG AAACTAGCAG CGCACTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCTATT TGCCTCAATG CATTCAGAGA CCAGGCTGTG 
GGCACTCCAG AGACCTGTGC CCACTATTTC TGCTTGGACT GTATTGTCGA ATGGTCTAGG 
AATGCCAACT CCTGTCCAGT TGATCGAACA GTATTTAAAT GTATTTGTAT TCGAGCTCGA 
CTTGATGGTA AAATTTTAAA GAAGATTCCA GTGGAAAACA CTAGAGCCCG TGAGGATGAG 
GAGGCTGAGG AGGAGGACCC GACCTTCTGT GAGGTGTGTG GTAGGAGTGA CCGTGAGGAC 
CGGCTCCTGC TCTGTGATGG CTGTGATGCT GGGTACCACA TGGAATGTTT AGATCCTCCT 
CTCCAGGAGG TACCTGTGGA TGAGTGGTTC TGCCCAGAGT GTGTCACCCC TGGTGCTGCC 
TCCACTCATG ACACAGCTCC TGTGAGTGAT GAGGAGGTCT CACTGTTACT GGCCGACGTG 
GTACCCACCA CCAGCAGGCT GCGGCCTCGT GTGGGCAGGA CCCGAGCCAT CGCCAGGACA 
CGACAGAGTG AGAGAGTGAG GGCCACTGTA AACCGGAACA GGATCTCATC TGCCAGAAGG 
GTTCAGCATG TGCCAAGATA CCTCATGTCT TCTCTGCTGG ATGAGACTAT CGAGGCTGTC 
GCCACTGGTC TGAGCACTGC TGTGTACCAG CGCTCCCTGA CTCCTCGTGT CCCTGCAAAA 
CGGAAAAGGA AGGCAGGGAG ACGGAAAAAA GTGCTTGGAA GAAAGACGAC CCGGTCTAGG 
TCATCTGTGA AAAGGAAGAG TGGGGGTCTG AGAGCCAAGA AACGCCAGCA CCGTGTGAGG 
AAGACAAAAG GGAGAAAGAT GAAGAATGAG GTTACAGCTC GTTCTCGAAT TGCACGGACA 
CTGGGCCTCC GCAGGCCTGT CCGTGGCACC AGCATGCCAT CAGTGTACAA ACCAGTAGAC 
CCCTCCCTGG GGCTAATGAG GGCAGATATC GGAGCAGCTT CTCTGTCACT GTTTGGAGAC 
CCCTACGAGC TGGACCCTTT TGACAGCAAT GAAGAACTGT CTGCAAATCT GCCTTCCCCT 
CTGAGTGCCA AGAGGAGAGT GCTGTCCCGC TCGGCCTTGC AGTCTCACCA GCCTGTAGCC 
AGGCCTGTTG CTATGGGGCT CTCCAGAAGG CAGCTCCCAG CTGTGGCCCC AGAGCCCAGT 
GTGGAGGAGG CTCCTGTTCC TGATCTGTTG GGGAGCATCC TGACTGGCCA GAGCCTCCTG 
ATGATGAGCA GCGCTGATGT TGTCATCCAC CGGGATGGCT CTCTTAGCGC AAAGAGGACA 
GCTCCTGTTT CTCTTCAACG AAATTCTGTG ACTCAATCCA GAGGAGAAGA TGAGTCCAGG 
CGCAGAGACA GCCTACAGCC TGAGGCATCA CCCTCAGGGA GCCCATCCAA TGTCGGACTC 
ACAGGAAGCA GGCCACAGAG CTCAGGGATA AGCTGTGGGG ACAGGTCGGC CCTACGCTGT 
ATCCCCGCCT ACTCAGTGGG GACACCTGCG AGGCCGGAGT CATCATCAAC CCCTGGTTCA 
AGTCAGGCTG GGAAGCTTTC AAATGGGAGC AGGCCTAGCC TGAAGCAGAA TAACAGCCCC 
CAGTTTAATG GCGCGAACAT GCAGGCCTTG CCTCTTCGTT CTGCCTCCTC TAAGATCTCA 
GCCACTCACT CTAACTCTTC ATCTAGAAAT ATAGTCCTTA GACAGTCCCT GAAAACAGCT 
CCCAGGAGAG CCGACATCTC ACAGTTGCCC AGAATACCAA AGATCCGTAG GAATGGCAGT 
GGCAGCCGAC AAGACCCTGC CCCAGCCCAT GGGCAGAGCG TGGAGCTCCC CAGCACCTGC 
ATTAGCCGCC TGACAGGAAG GGAGGGTGCT GGACAGCCAG GGCGAGGCCG AGCTGAGAAT 
GAGCCCAGAA ACAGGGGCCC TCAGGAGCCT GGTTCACACA CGGGTGGCTC TCGGCCTCCT 
GCCCCCAGCT CCCATGGCAA CTTGGCCCCT CTAGGGCCTT CAAGAGGGAA AGGCATTGGG 
TCAACCTTTG AGAGTTTCAG GATCAACATC CCCGGGAATA CTGCACACAG CAGCCAGCTG 
TCCAGCCCTG GCTTCTGTAA CACATTCCGG CCAGTGGATA GCAAAGTGCA GAGGAAGGAG 
AACCCTTCGC CACTCTTCTC CATCAAGAAG CCGAAGCAAC TCAAGAGTGA AATCTATGAC 
CCCTTTGACC CCACTGGCTC TGACTCTAGT CCTCCTACCA GCAGCCCAGA GAGTATTGGC 
CCAGGCCTTC TGCCATCTGA GATCACGAGA ACAATCTCCA TCAACAGCCC AAAAGCCCCA 
GCCTTCCAGG CTGTGCGCTG TGTTACCTCC TACAGGGTGG AAAGCATCTT TGATACTGAG 
GTGGAACCTG AGCCCCAGCC CCCTGGTGAG CCCATGTCTG GCATGTTCAA GTTCCTGGGC 
AAGGGGCCTG CTGAGGGAGC CTCTGACTTG GAGCAAGAAA GGCTAGGTGA GGTGGAGCAT 
CCAGAAATCG AGGGTTCCAT AGCCCAGACA CACAGGCCAT CCCCACAAGA CCCCTGGGAA 
GATGAGGATG GAATGTCCTG TACACCCTTC TTTGGTTCTG AGGAGCGGAC AGTGACATGT 
GTGACTGTCA TGGAGCCAGA TGTTCCACAG GTCACCACAC ATAGGATTGT AGAGCTGAGA 
ACCCCATCCC GATCCCGTTC CACCTCTAGC TCCCGAAGCA AGAAGAAAAC CAAGAAGAAG 
AGGGCTGCCA GGGAGCACCA GAGGACACGC TCTAGCACTC GCTCTGGCTC CAGGGACAGG 
ACCTCACGTT CGGTATCGCC CGTGGTCGAG GAGCACACCA AGAGGCACAG GGCCAAGACC 
AAGAGCCGGA GGTCTTCCAG CGACCGTGCC AGCAGCCAGG AGAGAGTGAA GAGGAGGAAG 
ACCAGGGACA GGGACAGGGA GCGGAGACGG GGCTCCTGGA GCCATGGCAG GTGCCGCAGG 
AAGTCCAGGT CTCACTCAGG CAGTCCTGGC AGTTCTTCCT GTGAGCGCCA TGAAAGCAGG 
AAGCGGAAGA GGCGACGCTC AGGCTCCAGG TCTCGGGGCA AGGACTGCTC TTCCCCCAGC 
AACCTAGAGC GGGCCAGGAG ACACAAGCAC CGGGAACGGA GCCGGAGCCG AGACAAGAAA 
GACAGTATGA CCTGGTCCCG GGAGAGGAGG AGGTGGAGGT CTAGATCACC AAGCTTGGAG 
CATAGGCCAC GGCGGCCACG TTCCCGAGAG AAGCGGCCCC GTTCCCCAGA GAAGGCTCCA 
GCTGTGAAGG AAGCTTCCCC AGCCCCCACT GCTCAGGAGG AGCTGAGGCA AGATGAAGAC 
CGCCCGGCTA AACCTTCAGT CTCCGAGGAA GTGAGTGTCC TGCCAGAGGT GGTTGTGGCT 
GACCTGAACC CCCCAGAAAT GCCCCCTGTC CTGGCAGTGC CAGCTGAATC TGTGCCTGAG 
GACTTGGACT ATGGTGACTC TGTGGAAGCA GGGCACGTCT TTGAGGATTT TTCAAGTGAA 
GCCATCTTTA TCCAGCTTGA TGACATGAGT TCACCTCCTT CCCCTGAGAG TACAGACTCC 
TCCCCAGAAC GAGACTTCCC ACCAAATCCT GTGGTGCCCC CAGCCATCCA GCCACAGGAT 
GCCAGTCTGG CTGCCATCCA GAGGGAAGTG TCCCTGATCC ACAGTGAAGA CATCTCTCAG 
CCCACACCAC AGGCAGAGGG CCCTTCAGAC CAGTACCTGC TCAGGCAGGA CGCTGTAGAG 
ACCATTGCCA TGACTCCCAG CACCATGGGT GTGGTACCCA TGGGAAAAGA CAGCCCCTTG 
CTGAGTGGGA GAGGGTGTGA AGCAGTCAGG TCCAAGGAGA CAGTGGTCCA GGCCCCTCTG 
CTACGGGCCA GGGCCCTTGT AAAAAGAGTC ACCTGGAACC TACAGGAGGC AGAGGATGGT 
ACTCCAGCCC TGGACAGAGT TCCAAGGACA CCACTTCAGA GGCCACAGAG GCCCCGGGAA 
GGAGACTGGG ATGCAGAGGA CGGGGCCCTC GTAGGAGTTC AGCAGGCACC ATTCTCCGAG 
CTGCCCCCTC CCATCCACGT GCTCCAGGAG TCTGGGTTAC CTGATGCAGA CCCCTCTCAG 
CCCCCTGGTG CTTCCAGGGC AGAAGAGGTG CCAGCTGTTG GGACTCTGCA CTCAGCAGAG 
GGTATTCTTG CCCAAGTTTA CAACCCCAAC ATGCCACCAC CCCTGGCTCA GCCATCAAGC 
ATCCTGCCCT ACACCCTCGT CAGTCAGCCC TCGGTCCAGT TAATCCTGCA GGGGACCCTT 
CCCCTGGCTG GCTGTGGCAC AGCACAGACC CTGGCCCCAG TGCCTAATGT TCCAGCTGTA 
GCCTCAGAGC TGGCTGTTCT AACCAACAAC AGCAGCTCTG AGGAGAGGAC CAGCACTCCC 
AAGCCAGCCG CCGAGAAGAC CAAGAAGGAA GAGTACTTGA AGAAGCTGCA CATGCAGGAG 
CGGGCTGTGG AGGAGGTGAA GCTGGCCATT AAGCCCTTCT ACCAGAAGAG AGAGGTGACC 
AAGGAGGAAT ACAAGGACAT CCTGCGGAAA GCTGTGCAGA AGATCTGCCA CAGCAAGAGC 
GGCGAGATCA ACCCTGTGAA GGTGGCCAAT CTGGTAAAGG CCTATGTGGA CAAGTATAGG 
CACATGCGCA GGCACAAGAA GACTGAAGCT GGGGAGGAGC CACCTGCCCA GGGTGCCGAG 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_008854395.3 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008852617.1 Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X3, mRNA.

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CloneID	ONa36153
Clone ID Related Accession (Same CDS sequence)	XM_008854395.2 , XM_008854395.3
Accession Version	XM_008854395.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	PHD and RING finger domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008356261.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008854395.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGTTTGGA TGAGCTTGTG GCCCACAGCC CTGGGCCAAA TGGACCTCCA 
AGAGTCGTCC CCTCAGAACT GGCCAGTGAA GCTGAAAGTG GCAATGGTCA CAGTGGAGAC 
TCTGAAGACT ACAGTGGCAG TGAGCATGAA GACAACACAG ATGGGGAAGA AACAGAGGGG 
TTGTCTGAGG AGGAAGACCC AGAAGATAGA TCTGGTTCTG AAGATTCAGA AGATGGAATA 
GAAACATCAG TGGCAGCAGT GGGTACTCAA GGGAAGCTGG AAACTAGCAG CGCACTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCTATT TGCCTCAATG CATTCAGAGA CCAGGCTGTG 
GGCACTCCAG AGACCTGTGC CCACTATTTC TGCTTGGACT GTATTGTCGA ATGGTCTAGG 
AATGCCAACT CCTGTCCAGT TGATCGAACA GTATTTAAAT GTATTTGTAT TCGAGCTCGA 
CTTGATGGTA AAATTTTAAA GAAGATTCCA GTGGAAAACA CTAGAGCCCG TGAGGATGAG 
GAGGCTGAGG AGGAGGACCC GACCTTCTGT GAGGTGTGTG GTAGGAGTGA CCGTGAGGAC 
CGGCTCCTGC TCTGTGATGG CTGTGATGCT GGGTACCACA TGGAATGTTT AGATCCTCCT 
CTCCAGGAGG TACCTGTGGA TGAGTGGTTC TGCCCAGAGT GTGTCACCCC TGGTGCTGCC 
TCCACTCATG ACACAGCTCC TGTGAGTGAT GAGGAGGTCT CACTGTTACT GGCCGACGTG 
GTACCCACCA CCAGCAGGCT GCGGCCTCGT GTGGGCAGGA CCCGAGCCAT CGCCAGGACA 
CGACAGAGTG AGAGAGTGAG GGCCACTGTA AACCGGAACA GGATCTCATC TGCCAGAAGG 
GTTCAGCATG TGCCAAGATA CCTCATGTCT TCTCTGCTGG ATGAGACTAT CGAGGCTGTC 
GCCACTGGTC TGAGCACTGC TGTGTACCAG CGCTCCCTGA CTCCTCGTGT CCCTGCAAAA 
CGGAAAAGGA AGGCAGGGAG ACGGAAAAAA GTGCTTGGAA GAAAGACGAC CCGGTCTAGG 
TCATCTGTGA AAAGGAAGAG TGGGGGTCTG AGAGCCAAGA AACGCCAGCA CCGTGTGAGG 
AAGACAAAAG GGAGAAAGAT GAAGAATGAG GTTACAGCTC GTTCTCGAAT TGCACGGACA 
CTGGGCCTCC GCAGGCCTGT CCGTGGCACC AGCATGCCAT CAGTGTACAA ACCAGTAGAC 
CCCTCCCTGG GGCTAATGAG GGCAGATATC GGAGCAGCTT CTCTGTCACT GTTTGGAGAC 
CCCTACGAGC TGGACCCTTT TGACAGCAAT GAAGAACTGT CTGCAAATCT GCCTTCCCCT 
CTGAGTGCCA AGAGGAGAGT GCTGTCCCGC TCGGCCTTGC AGTCTCACCA GCCTGTAGCC 
AGGCCTGTTG CTATGGGGCT CTCCAGAAGG CAGCTCCCAG CTGTGGCCCC AGAGCCCAGT 
GTGGAGGAGG CTCCTGTTCC TGATCTGTTG GGGAGCATCC TGACTGGCCA GAGCCTCCTG 
ATGATGAGCA GCGCTGATGT TGTCATCCAC CGGGATGGCT CTCTTAGCGC AAAGAGGACA 
GCTCCTGTTT CTCTTCAACG AAATTCTGTG ACTCAATCCA GAGGAGAAGA TGAGTCCAGG 
CGCAGAGACA GCCTACAGCC TGAGGCATCA CCCTCAGGGA GCCCATCCAA TGTCGGACTC 
ACAGGAAGCA GGCCACAGAG CTCAGGGATA AGCTGTGGGG ACAGGTCGGC CCTACGCTGT 
ATCCCCGCCT ACTCAGTGGG GACACCTGCG AGGCCGGAGT CATCATCAAC CCCTGGTTCA 
AGTCAGGCTG GGAAGCTTTC AAATGGGAGC AGGCCTAGCC TGAAGCAGAA TAACAGCCCC 
CAGTTTAATG GCGCGAACAT GCAGGCCTTG CCTCTTCGTT CTGCCTCCTC TAAGATCTCA 
GCCACTCACT CTAACTCTTC ATCTAGAAAT ATAGTCCTTA GACAGTCCCT GAAAACAGCT 
CCCAGGAGAG CCGACATCTC ACAGTTGCCC AGAATACCAA AGATCCGTAG GAATGGCAGT 
GGCAGCCGAC AAGACCCTGC CCCAGCCCAT GGGCAGAGCG TGGAGCTCCC CAGCACCTGC 
ATTAGCCGCC TGACAGGAAG GGAGGGTGCT GGACAGCCAG GGCGAGGCCG AGCTGAGAAT 
GAGCCCAGAA ACAGGGGCCC TCAGGAGCCT GGTTCACACA CGGGTGGCTC TCGGCCTCCT 
GCCCCCAGCT CCCATGGCAA CTTGGCCCCT CTAGGGCCTT CAAGAGGGAA AGGCATTGGG 
TCAACCTTTG AGAGTTTCAG GATCAACATC CCCGGGAATA CTGCACACAG CAGCCAGCTG 
TCCAGCCCTG GCTTCTGTAA CACATTCCGG CCAGTGGATA GCAAAGTGCA GAGGAAGGAG 
AACCCTTCGC CACTCTTCTC CATCAAGAAG CCGAAGCAAC TCAAGAGTGA AATCTATGAC 
CCCTTTGACC CCACTGGCTC TGACTCTAGT CCTCCTACCA GCAGCCCAGA GAGTATTGGC 
CCAGGCCTTC TGCCATCTGA GATCACGAGA ACAATCTCCA TCAACAGCCC AAAAGCCCCA 
GCCTTCCAGG CTGTGCGCTG TGTTACCTCC TACAGGGTGG AAAGCATCTT TGATACTGAG 
GTGGAACCTG AGCCCCAGCC CCCTGGTGAG CCCATGTCTG GCATGTTCAA GTTCCTGGGC 
AAGGGGCCTG CTGAGGGAGC CTCTGACTTG GAGCAAGAAA GGCTAGGTGA GGTGGAGCAT 
CCAGAAATCG AGGGTTCCAT AGCCCAGACA CACAGGCCAT CCCCACAAGA CCCCTGGGAA 
GATGAGGATG GAATGTCCTG TACACCCTTC TTTGGTTCTG AGGAGCGGAC AGTGACATGT 
GTGACTGTCA TGGAGCCAGA TGTTCCACAG GTCACCACAC ATAGGATTGT AGAGCTGAGA 
ACCCCATCCC GATCCCGTTC CACCTCTAGC TCCCGAAGCA AGAAGAAAAC CAAGAAGAAG 
AGGGCTGCCA GGGAGCACCA GAGGACACGC TCTAGCACTC GCTCTGGCTC CAGGGACAGG 
ACCTCACGTT CGGTATCGCC CGTGGTCGAG GAGCACACCA AGAGGCACAG GGCCAAGACC 
AAGAGCCGGA GGTCTTCCAG CGACCGTGCC AGCAGCCAGG AGAGAGTGAA GAGGAGGAAG 
ACCAGGGACA GGGACAGGGA GCGGAGACGG GGCTCCTGGA GCCATGGCAG GTGCCGCAGG 
AAGTCCAGGT CTCACTCAGG CAGTCCTGGC AGTTCTTCCT GTGAGCGCCA TGAAAGCAGG 
AAGCGGAAGA GGCGACGCTC AGGCTCCAGG TCTCGGGGCA AGGACTGCTC TTCCCCCAGC 
AACCTAGAGC GGGCCAGGAG ACACAAGCAC CGGGAACGGA GCCGGAGCCG AGACAAGAAA 
GACAGTATGA CCTGGTCCCG GGAGAGGAGG AGGTGGAGGT CTAGATCACC AAGCTTGGAG 
CATAGGCCAC GGCGGCCACG TTCCCGAGAG AAGCGGCCCC GTTCCCCAGA GAAGGCTCCA 
GCTGTGAAGG AAGCTTCCCC AGCCCCCACT GCTCAGGAGG AGCTGAGGCA AGATGAAGAC 
CGCCCGGCTA AACCTTCAGT CTCCGAGGAA GTGAGTGTCC TGCCAGAGGT GGTTGTGGCT 
GACCTGAACC CCCCAGAAAT GCCCCCTGTC CTGGCAGTGC CAGCTGAATC TGTGCCTGAG 
GACTTGGACT ATGGTGACTC TGTGGAAGCA GGGCACGTCT TTGAGGATTT TTCAAGTGAA 
GCCATCTTTA TCCAGCTTGA TGACATGAGT TCACCTCCTT CCCCTGAGAG TACAGACTCC 
TCCCCAGAAC GAGACTTCCC ACCAAATCCT GTGGTGCCCC CAGCCATCCA GCCACAGGAT 
GCCAGTCTGG CTGCCATCCA GAGGGAAGTG TCCCTGATCC ACAGTGAAGA CATCTCTCAG 
CCCACACCAC AGGCAGAGGG CCCTTCAGAC CAGTACCTGC TCAGGCAGGA CGCTGTAGAG 
ACCATTGCCA TGACTCCCAG CACCATGGGT GTGGTACCCA TGGGAAAAGA CAGCCCCTTG 
CTGAGTGGGA GAGGGTGTGA AGCAGTCAGG TCCAAGGAGA CAGTGGTCCA GGCCCCTCTG 
CTACGGGCCA GGGCCCTTGT AAAAAGAGTC ACCTGGAACC TACAGGAGGC AGAGGATGGT 
ACTCCAGCCC TGGACAGAGT TCCAAGGACA CCACTTCAGA GGCCACAGAG GCCCCGGGAA 
GGAGACTGGG ATGCAGAGGA CGGGGCCCTC GTAGGAGTTC AGCAGGCACC ATTCTCCGAG 
CTGCCCCCTC CCATCCACGT GCTCCAGGAG TCTGGGTTAC CTGATGCAGA CCCCTCTCAG 
CCCCCTGGTG CTTCCAGGGC AGAAGAGGTG CCAGCTGTTG GGACTCTGCA CTCAGCAGAG 
GGTATTCTTG CCCAAGTTTA CAACCCCAAC ATGCCACCAC CCCTGGCTCA GCCATCAAGC 
ATCCTGCCCT ACACCCTCGT CAGTCAGCCC TCGGTCCAGT TAATCCTGCA GGGGACCCTT 
CCCCTGGCTG GCTGTGGCAC AGCACAGACC CTGGCCCCAG TGCCTAATGT TCCAGCTGTA 
GCCTCAGAGC TGGCTGTTCT AACCAACAAC AGCAGCTCTG AGGAGAGGAC CAGCACTCCC 
AAGCCAGCCG CCGAGAAGAC CAAGAAGGAA GAGTACTTGA AGAAGCTGCA CATGCAGGAG 
CGGGCTGTGG AGGAGGTGAA GCTGGCCATT AAGCCCTTCT ACCAGAAGAG AGAGGTGACC 
AAGGAGGAAT ACAAGGACAT CCTGCGGAAA GCTGTGCAGA AGATCTGCCA CAGCAAGAGC 
GGCGAGATCA ACCCTGTGAA GGTGGCCAAT CTGGTAAAGG CCTATGTGGA CAAGTATAGG 
CACATGCGCA GGCACAAGAA GACTGAAGCT GGGGAGGAGC CACCTGCCCA GGGTGCCGAG 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008852617.1
CDS	388..5433
Translation	MDDDSLDELVAHSPGPNGPPRVVPSELASEAESGNGHSGDSEDYSGSEHEDNTDGEETEGLSEEEDPEDRSGSEDSEDGIETSVAAVGTQGKLETSSALNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIVEWSRNANSCPVDRTVFKCICIRARLDGKILKKIPVENTRAREDEEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECVTPGAASTHDTAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRSLTPRVPAKRKRKAGRRKKVLGRKTTRSRSSVKRKSGGLRAKKRQHRVRKTKGRKMKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEELSANLPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILTGQSLLMMSSADVVIHRDGSLSAKRTAPVSLQRNSVTQSRGEDESRRRDSLQPEASPSGSPSNVGLTGSRPQSSGISCGDRSALRCIPAYSVGTPARPESSSTPGSSQAGKLSNGSRPSLKQNNSPQFNGANMQALPLRSASSKISATHSNSSSRNIVLRQSLKTAPRRADISQLPRIPKIRRNGSGSRQDPAPAHGQSVELPSTCISRLTGREGAGQPGRGRAENEPRNRGPQEPGSHTGGSRPPAPSSHGNLAPLGPSRGKGIGSTFESFRINIPGNTAHSSQLSSPGFCNTFRPVDSKVQRKENPSPLFSIKKPKQLKSEIYDPFDPTGSDSSPPTSSPESIGPGLLPSEITRTISINSPKAPAFQAVRCVTSYRVESIFDTEVEPEPQPPGEPMSGMFKFLGKGPAEGASDLEQERLGEVEHPEIEGSIAQTHRPSPQDPWEDEDGMSCTPFFGSEERTVTCVTVMEPDVPQVTTHRIVELRTPSRSRSTSSSRSKKKTKKKRAAREHQRTRSSTRSGSRDRTSRSVSPVVEEHTKRHRAKTKSRRSSSDRASSQERVKRRKTRDRDRERRRGSWSHGRCRRKSRSHSGSPGSSSCERHESRKRKRRRSGSRSRGKDCSSPSNLERARRHKHRERSRSRDKKDSMTWSRERRRWRSRSPSLEHRPRRPRSREKRPRSPEKAPAVKEASPAPTAQEELRQDEDRPAKPSVSEEVSVLPEVVVADLNPPEMPPVLAVPAESVPEDLDYGDSVEAGHVFEDFSSEAIFIQLDDMSSPPSPESTDSSPERDFPPNPVVPPAIQPQDASLAAIQREVSLIHSEDISQPTPQAEGPSDQYLLRQDAVETIAMTPSTMGVVPMGKDSPLLSGRGCEAVRSKETVVQAPLLRARALVKRVTWNLQEAEDGTPALDRVPRTPLQRPQRPREGDWDAEDGALVGVQQAPFSELPPPIHVLQESGLPDADPSQPPGASRAEEVPAVGTLHSAEGILAQVYNPNMPPPLAQPSSILPYTLVSQPSVQLILQGTLPLAGCGTAQTLAPVPNVPAVASELAVLTNNSSSEERTSTPKPAAEKTKKEEYLKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEAGEEPPAQGAET

Target ORF information:

RefSeq Version	XM_008854395.3
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008854395.3

ORF Insert Sequence:

ATGGATGACG ACAGTTTGGA TGAGCTTGTG GCCCACAGCC CTGGGCCAAA TGGACCTCCA 
AGAGTCGTCC CCTCAGAACT GGCCAGTGAA GCTGAAAGTG GCAATGGTCA CAGTGGAGAC 
TCTGAAGACT ACAGTGGCAG TGAGCATGAA GACAACACAG ATGGGGAAGA AACAGAGGGG 
TTGTCTGAGG AGGAAGACCC AGAAGATAGA TCTGGTTCTG AAGATTCAGA AGATGGAATA 
GAAACATCAG TGGCAGCAGT GGGTACTCAA GGGAAGCTGG AAACTAGCAG CGCACTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCTATT TGCCTCAATG CATTCAGAGA CCAGGCTGTG 
GGCACTCCAG AGACCTGTGC CCACTATTTC TGCTTGGACT GTATTGTCGA ATGGTCTAGG 
AATGCCAACT CCTGTCCAGT TGATCGAACA GTATTTAAAT GTATTTGTAT TCGAGCTCGA 
CTTGATGGTA AAATTTTAAA GAAGATTCCA GTGGAAAACA CTAGAGCCCG TGAGGATGAG 
GAGGCTGAGG AGGAGGACCC GACCTTCTGT GAGGTGTGTG GTAGGAGTGA CCGTGAGGAC 
CGGCTCCTGC TCTGTGATGG CTGTGATGCT GGGTACCACA TGGAATGTTT AGATCCTCCT 
CTCCAGGAGG TACCTGTGGA TGAGTGGTTC TGCCCAGAGT GTGTCACCCC TGGTGCTGCC 
TCCACTCATG ACACAGCTCC TGTGAGTGAT GAGGAGGTCT CACTGTTACT GGCCGACGTG 
GTACCCACCA CCAGCAGGCT GCGGCCTCGT GTGGGCAGGA CCCGAGCCAT CGCCAGGACA 
CGACAGAGTG AGAGAGTGAG GGCCACTGTA AACCGGAACA GGATCTCATC TGCCAGAAGG 
GTTCAGCATG TGCCAAGATA CCTCATGTCT TCTCTGCTGG ATGAGACTAT CGAGGCTGTC 
GCCACTGGTC TGAGCACTGC TGTGTACCAG CGCTCCCTGA CTCCTCGTGT CCCTGCAAAA 
CGGAAAAGGA AGGCAGGGAG ACGGAAAAAA GTGCTTGGAA GAAAGACGAC CCGGTCTAGG 
TCATCTGTGA AAAGGAAGAG TGGGGGTCTG AGAGCCAAGA AACGCCAGCA CCGTGTGAGG 
AAGACAAAAG GGAGAAAGAT GAAGAATGAG GTTACAGCTC GTTCTCGAAT TGCACGGACA 
CTGGGCCTCC GCAGGCCTGT CCGTGGCACC AGCATGCCAT CAGTGTACAA ACCAGTAGAC 
CCCTCCCTGG GGCTAATGAG GGCAGATATC GGAGCAGCTT CTCTGTCACT GTTTGGAGAC 
CCCTACGAGC TGGACCCTTT TGACAGCAAT GAAGAACTGT CTGCAAATCT GCCTTCCCCT 
CTGAGTGCCA AGAGGAGAGT GCTGTCCCGC TCGGCCTTGC AGTCTCACCA GCCTGTAGCC 
AGGCCTGTTG CTATGGGGCT CTCCAGAAGG CAGCTCCCAG CTGTGGCCCC AGAGCCCAGT 
GTGGAGGAGG CTCCTGTTCC TGATCTGTTG GGGAGCATCC TGACTGGCCA GAGCCTCCTG 
ATGATGAGCA GCGCTGATGT TGTCATCCAC CGGGATGGCT CTCTTAGCGC AAAGAGGACA 
GCTCCTGTTT CTCTTCAACG AAATTCTGTG ACTCAATCCA GAGGAGAAGA TGAGTCCAGG 
CGCAGAGACA GCCTACAGCC TGAGGCATCA CCCTCAGGGA GCCCATCCAA TGTCGGACTC 
ACAGGAAGCA GGCCACAGAG CTCAGGGATA AGCTGTGGGG ACAGGTCGGC CCTACGCTGT 
ATCCCCGCCT ACTCAGTGGG GACACCTGCG AGGCCGGAGT CATCATCAAC CCCTGGTTCA 
AGTCAGGCTG GGAAGCTTTC AAATGGGAGC AGGCCTAGCC TGAAGCAGAA TAACAGCCCC 
CAGTTTAATG GCGCGAACAT GCAGGCCTTG CCTCTTCGTT CTGCCTCCTC TAAGATCTCA 
GCCACTCACT CTAACTCTTC ATCTAGAAAT ATAGTCCTTA GACAGTCCCT GAAAACAGCT 
CCCAGGAGAG CCGACATCTC ACAGTTGCCC AGAATACCAA AGATCCGTAG GAATGGCAGT 
GGCAGCCGAC AAGACCCTGC CCCAGCCCAT GGGCAGAGCG TGGAGCTCCC CAGCACCTGC 
ATTAGCCGCC TGACAGGAAG GGAGGGTGCT GGACAGCCAG GGCGAGGCCG AGCTGAGAAT 
GAGCCCAGAA ACAGGGGCCC TCAGGAGCCT GGTTCACACA CGGGTGGCTC TCGGCCTCCT 
GCCCCCAGCT CCCATGGCAA CTTGGCCCCT CTAGGGCCTT CAAGAGGGAA AGGCATTGGG 
TCAACCTTTG AGAGTTTCAG GATCAACATC CCCGGGAATA CTGCACACAG CAGCCAGCTG 
TCCAGCCCTG GCTTCTGTAA CACATTCCGG CCAGTGGATA GCAAAGTGCA GAGGAAGGAG 
AACCCTTCGC CACTCTTCTC CATCAAGAAG CCGAAGCAAC TCAAGAGTGA AATCTATGAC 
CCCTTTGACC CCACTGGCTC TGACTCTAGT CCTCCTACCA GCAGCCCAGA GAGTATTGGC 
CCAGGCCTTC TGCCATCTGA GATCACGAGA ACAATCTCCA TCAACAGCCC AAAAGCCCCA 
GCCTTCCAGG CTGTGCGCTG TGTTACCTCC TACAGGGTGG AAAGCATCTT TGATACTGAG 
GTGGAACCTG AGCCCCAGCC CCCTGGTGAG CCCATGTCTG GCATGTTCAA GTTCCTGGGC 
AAGGGGCCTG CTGAGGGAGC CTCTGACTTG GAGCAAGAAA GGCTAGGTGA GGTGGAGCAT 
CCAGAAATCG AGGGTTCCAT AGCCCAGACA CACAGGCCAT CCCCACAAGA CCCCTGGGAA 
GATGAGGATG GAATGTCCTG TACACCCTTC TTTGGTTCTG AGGAGCGGAC AGTGACATGT 
GTGACTGTCA TGGAGCCAGA TGTTCCACAG GTCACCACAC ATAGGATTGT AGAGCTGAGA 
ACCCCATCCC GATCCCGTTC CACCTCTAGC TCCCGAAGCA AGAAGAAAAC CAAGAAGAAG 
AGGGCTGCCA GGGAGCACCA GAGGACACGC TCTAGCACTC GCTCTGGCTC CAGGGACAGG 
ACCTCACGTT CGGTATCGCC CGTGGTCGAG GAGCACACCA AGAGGCACAG GGCCAAGACC 
AAGAGCCGGA GGTCTTCCAG CGACCGTGCC AGCAGCCAGG AGAGAGTGAA GAGGAGGAAG 
ACCAGGGACA GGGACAGGGA GCGGAGACGG GGCTCCTGGA GCCATGGCAG GTGCCGCAGG 
AAGTCCAGGT CTCACTCAGG CAGTCCTGGC AGTTCTTCCT GTGAGCGCCA TGAAAGCAGG 
AAGCGGAAGA GGCGACGCTC AGGCTCCAGG TCTCGGGGCA AGGACTGCTC TTCCCCCAGC 
AACCTAGAGC GGGCCAGGAG ACACAAGCAC CGGGAACGGA GCCGGAGCCG AGACAAGAAA 
GACAGTATGA CCTGGTCCCG GGAGAGGAGG AGGTGGAGGT CTAGATCACC AAGCTTGGAG 
CATAGGCCAC GGCGGCCACG TTCCCGAGAG AAGCGGCCCC GTTCCCCAGA GAAGGCTCCA 
GCTGTGAAGG AAGCTTCCCC AGCCCCCACT GCTCAGGAGG AGCTGAGGCA AGATGAAGAC 
CGCCCGGCTA AACCTTCAGT CTCCGAGGAA GTGAGTGTCC TGCCAGAGGT GGTTGTGGCT 
GACCTGAACC CCCCAGAAAT GCCCCCTGTC CTGGCAGTGC CAGCTGAATC TGTGCCTGAG 
GACTTGGACT ATGGTGACTC TGTGGAAGCA GGGCACGTCT TTGAGGATTT TTCAAGTGAA 
GCCATCTTTA TCCAGCTTGA TGACATGAGT TCACCTCCTT CCCCTGAGAG TACAGACTCC 
TCCCCAGAAC GAGACTTCCC ACCAAATCCT GTGGTGCCCC CAGCCATCCA GCCACAGGAT 
GCCAGTCTGG CTGCCATCCA GAGGGAAGTG TCCCTGATCC ACAGTGAAGA CATCTCTCAG 
CCCACACCAC AGGCAGAGGG CCCTTCAGAC CAGTACCTGC TCAGGCAGGA CGCTGTAGAG 
ACCATTGCCA TGACTCCCAG CACCATGGGT GTGGTACCCA TGGGAAAAGA CAGCCCCTTG 
CTGAGTGGGA GAGGGTGTGA AGCAGTCAGG TCCAAGGAGA CAGTGGTCCA GGCCCCTCTG 
CTACGGGCCA GGGCCCTTGT AAAAAGAGTC ACCTGGAACC TACAGGAGGC AGAGGATGGT 
ACTCCAGCCC TGGACAGAGT TCCAAGGACA CCACTTCAGA GGCCACAGAG GCCCCGGGAA 
GGAGACTGGG ATGCAGAGGA CGGGGCCCTC GTAGGAGTTC AGCAGGCACC ATTCTCCGAG 
CTGCCCCCTC CCATCCACGT GCTCCAGGAG TCTGGGTTAC CTGATGCAGA CCCCTCTCAG 
CCCCCTGGTG CTTCCAGGGC AGAAGAGGTG CCAGCTGTTG GGACTCTGCA CTCAGCAGAG 
GGTATTCTTG CCCAAGTTTA CAACCCCAAC ATGCCACCAC CCCTGGCTCA GCCATCAAGC 
ATCCTGCCCT ACACCCTCGT CAGTCAGCCC TCGGTCCAGT TAATCCTGCA GGGGACCCTT 
CCCCTGGCTG GCTGTGGCAC AGCACAGACC CTGGCCCCAG TGCCTAATGT TCCAGCTGTA 
GCCTCAGAGC TGGCTGTTCT AACCAACAAC AGCAGCTCTG AGGAGAGGAC CAGCACTCCC 
AAGCCAGCCG CCGAGAAGAC CAAGAAGGAA GAGTACTTGA AGAAGCTGCA CATGCAGGAG 
CGGGCTGTGG AGGAGGTGAA GCTGGCCATT AAGCCCTTCT ACCAGAAGAG AGAGGTGACC 
AAGGAGGAAT ACAAGGACAT CCTGCGGAAA GCTGTGCAGA AGATCTGCCA CAGCAAGAGC 
GGCGAGATCA ACCCTGTGAA GGTGGCCAAT CTGGTAAAGG CCTATGTGGA CAAGTATAGG 
CACATGCGCA GGCACAAGAA GACTGAAGCT GGGGAGGAGC CACCTGCCCA GGGTGCCGAG 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_017803574.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017659063.1 Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X3, mRNA.

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CloneID	ONa36154
Clone ID Related Accession (Same CDS sequence)	XM_017803574.1
Accession Version	XM_017803574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	PHD and RING finger domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008356261.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGTTTGGA TGAGCTTGTG GCCCACAGCC CTGGGCCAAA TGGACCTCCA 
AGAGTCGTCC CCTCAGAACT GGCCAGTGAA GCTGAAGAAA GTGGCAATGG TCACAGTGGA 
GACTCTGAAG ACTACAGTGG CAGTGAGCAT GAAGACAACA CAGATGGGGA AGAAACAGAG 
GGGTTGTCTG AGGAGGAAGA CCCAGAAGAT AGATCTGGTT CTGAAGATTC AGAAGATGGA 
ATAGAAACAT CAGTGGCAGC AGTGGGTACT CAAGGGAAGC TGGAAACTAG CAGCGCACTC 
AATTCTGATG ATGATGCAGA GAGCTGCCCT ATTTGCCTCA ATGCATTCAG AGACCAGGCT 
GTGGGCACTC CAGAGACCTG TGCCCACTAT TTCTGCTTGG ACTGTATTGT CGAATGGTCT 
AGGAATGCCA ACTCCTGTCC AGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGAGCT 
CGACTTGATG GTAAAATTTT AAAGAAGATT CCAGTGGAAA ACACTAGAGC CCGTGAGGAT 
GAGGAGGCTG AGGAGGAGGA CCCGACCTTC TGTGAGGTGT GTGGTAGGAG TGACCGTGAG 
GACCGGCTCC TGCTCTGTGA TGGCTGTGAT GCTGGGTACC ACATGGAATG TTTAGATCCT 
CCTCTCCAGG AGGTACCTGT GGATGAGTGG TTCTGCCCAG AGTGTGTCAC CCCTGGTGCT 
GCCTCCACTC ATGCTCCTGT GAGTGATGAG GAGGTCTCAC TGTTACTGGC CGACGTGGTA 
CCCACCACCA GCAGGCTGCG GCCTCGTGTG GGCAGGACCC GAGCCATCGC CAGGACACGA 
CAGAGTGAGA GAGTGAGGGC CACTGTAAAC CGGAACAGGA TCTCATCTGC CAGAAGGGTT 
CAGCATGTGC CAAGATACCT CATGTCTTCT CTGCTGGATG AGACTATCGA GGCTGTCGCC 
ACTGGTCTGA GCACTGCTGT GTACCAGCGC TCCCTGACTC CTCGTGTCCC TGCAAAACGG 
AAAAGGAAGG CAGGGAGACG GAAAAAAGTG CTTGGAAGAA AGACGACCCG GTCTAGGTCA 
TCTGTGAAAA GGAAGAGTGG GGGTCTGAGA GCCAAGAAAC GCCAGCACCG TGTGAGGAAG 
ACAAAAGGGA GAAAGATGAA GAATGAGGTT ACAGCTCGTT CTCGAATTGC ACGGACACTG 
GGCCTCCGCA GGCCTGTCCG TGGCACCAGC ATGCCATCAG TGTACAAACC AGTAGACCCC 
TCCCTGGGGC TAATGAGGGC AGATATCGGA GCAGCTTCTC TGTCACTGTT TGGAGACCCC 
TACGAGCTGG ACCCTTTTGA CAGCAATGAA GAACTGTCTG CAAATCTGCC TTCCCCTCTG 
AGTGCCAAGA GGAGAGTGCT GTCCCGCTCG GCCTTGCAGT CTCACCAGCC TGTAGCCAGG 
CCTGTTGCTA TGGGGCTCTC CAGAAGGCAG CTCCCAGCTG TGGCCCCAGA GCCCAGTGTG 
GAGGAGGCTC CTGTTCCTGA TCTGTTGGGG AGCATCCTGA CTGGCCAGAG CCTCCTGATG 
ATGAGCAGCG CTGATGTTGT CATCCACCGG GATGGCTCTC TTAGCGCAAA GAGGACAGCT 
CCTGTTTCTC TTCAACGAAA TTCTGTGACT CAATCCAGAG GAGAAGATGA GTCCAGGCGC 
AGAGACAGCC TACAGCCTGA GGCATCACCC TCAGGGAGCC CATCCAATGT CGGACTCACA 
GGAAGCAGGC CACAGAGCTC AGGGATAAGC TGTGGGGACA GGTCGGCCCT ACGCTGTATC 
CCCGCCTACT CAGTGGGGAC ACCTGCGAGG CCGGAGTCAT CATCAACCCC TGGTTCAAGT 
CAGGCTGGGA AGCTTTCAAA TGGGAGCAGG CCTAGCCTGA AGCAGAATAA CAGCCCCCAG 
TTTAATGGCG CGAACATGCA GGCCTTGCCT CTTCGTTCTG CCTCCTCTAA GATCTCAGCC 
ACTCACTCTA ACTCTTCATC TAGAAATATA GTCCTTAGAC AGTCCCTGAA AACAGCTCCC 
AGGAGAGCCG ACATCTCACA GTTGCCCAGA ATACCAAAGA TCCGTAGGAA TGGCAGTGGC 
AGCCGACAAG ACCCTGCCCC AGCCCATGGG CAGAGCGTGG AGCTCCCCAG CACCTGCATT 
AGCCGCCTGA CAGGAAGGGA GGGTGCTGGA CAGCCAGGGC GAGGCCGAGC TGAGAATGAG 
CCCAGAAACA GGGGCCCTCA GGAGCCTGGT TCACACACGG GTGGCTCTCG GCCTCCTGCC 
CCCAGCTCCC ATGGCAACTT GGCCCCTCTA GGGCCTTCAA GAGGGAAAGG CATTGGGTCA 
ACCTTTGAGA GTTTCAGGAT CAACATCCCC GGGAATACTG CACACAGCAG CCAGCTGTCC 
AGCCCTGGCT TCTGTAACAC ATTCCGGCCA GTGGATAGCA AAGTGCAGAG GAAGGAGAAC 
CCTTCGCCAC TCTTCTCCAT CAAGAAGCCG AAGCAACTCA AGAGTGAAAT CTATGACCCC 
TTTGACCCCA CTGGCTCTGA CTCTAGTCCT CCTACCAGCA GCCCAGAGAG TATTGGCCCA 
GGCCTTCTGC CATCTGAGAT CACGAGAACA ATCTCCATCA ACAGCCCAAA AGCCCCAGCC 
TTCCAGGCTG TGCGCTGTGT TACCTCCTAC AGGGTGGAAA GCATCTTTGA TACTGAGGTG 
GAACCTGAGC CCCAGCCCCC TGGTGAGCCC ATGTCTGGCA TGTTCAAGTT CCTGGGCAAG 
GGGCCTGCTG AGGGAGCCTC TGACTTGGAG CAAGAAAGGC TAGGTGAGGT GGAGCATCCA 
GAAATCGAGG GTTCCATAGC CCAGACACAC AGGCCATCCC CACAAGACCC CTGGGAAGAT 
GAGGATGGAA TGTCCTGTAC ACCCTTCTTT GGTTCTGAGG AGCGGACAGT GACATGTGTG 
ACTGTCATGG AGCCAGATGT TCCACAGGTC ACCACACATA GGATTGTAGA GCTGAGAACC 
CCATCCCGAT CCCGTTCCAC CTCTAGCTCC CGAAGCAAGA AGAAAACCAA GAAGAAGAGG 
GCTGCCAGGG AGCACCAGAG GACACGCTCT AGCACTCGCT CTGGCTCCAG GGACAGGACC 
TCACGTTCGG TATCGCCCGT GGTCGAGGAG CACACCAAGA GGCACAGGGC CAAGACCAAG 
AGCCGGAGGT CTTCCAGCGA CCGTGCCAGC AGCCAGGAGA GAGTGAAGAG GAGGAAGACC 
AGGGACAGGG ACAGGGAGCG GAGACGGGGC TCCTGGAGCC ATGGCAGGTG CCGCAGGAAG 
TCCAGGTCTC ACTCAGGCAG TCCTGGCAGT TCTTCCTGTG AGCGCCATGA AAGCAGGAAG 
CGGAAGAGGC GACGCTCAGG CTCCAGGTCT CGGGGCAAGG ACTGCTCTTC CCCCAGCAAC 
CTAGAGCGGG CCAGGAGACA CAAGCACCGG GAACGGAGCC GGAGCCGAGA CAAGAAAGAC 
AGTATGACCT GGTCCCGGGA GAGGAGGAGG TGGAGGTCTA GATCACCAAG CTTGGAGCAT 
AGGCCACGGC GGCCACGTTC CCGAGAGAAG CGGCCCCGTT CCCCAGAGAA GGCTCCAGCT 
GTGAAGGAAG CTTCCCCAGC CCCCACTGCT CAGGAGGAGC TGAGGCAAGA TGAAGACCGC 
CCGGCTAAAC CTTCAGTCTC CGAGGAAGTG AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCCC CAGAAATGCC CCCTGTCCTG GCAGTGCCAG CTGAATCTGT GCCTGAGGAC 
TTGGACTATG GTGACTCTGT GGAAGCAGGG CACGTCTTTG AGGATTTTTC AAGTGAAGCC 
ATCTTTATCC AGCTTGATGA CATGAGTTCA CCTCCTTCCC CTGAGAGTAC AGACTCCTCC 
CCAGAACGAG ACTTCCCACC AAATCCTGTG GTGCCCCCAG CCATCCAGCC ACAGGATGCC 
AGTCTGGCTG CCATCCAGAG GGAAGTGTCC CTGATCCACA GTGAAGACAT CTCTCAGCCC 
ACACCACAGG CAGAGGGCCC TTCAGACCAG TACCTGCTCA GGCAGGACGC TGTAGAGACC 
ATTGCCATGA CTCCCAGCAC CATGGGTGTG GTACCCATGG GAAAAGACAG CCCCTTGCTG 
AGTGGGAGAG GGTGTGAAGC AGTCAGGTCC AAGGAGACAG TGGTCCAGGC CCCTCTGCTA 
CGGGCCAGGG CCCTTGTAAA AAGAGTCACC TGGAACCTAC AGGAGGCAGA GGATGGTACT 
CCAGCCCTGG ACAGAGTTCC AAGGACACCA CTTCAGAGGC CACAGAGGCC CCGGGAAGGA 
GACTGGGATG CAGAGGACGG GGCCCTCGTA GGAGTTCAGC AGGCACCATT CTCCGAGCTG 
CCCCCTCCCA TCCACGTGCT CCAGGAGTCT GGGTTACCTG ATGCAGACCC CTCTCAGCCC 
CCTGGTGCTT CCAGGGCAGA AGAGGTGCCA GCTGTTGGGA CTCTGCACTC AGCAGAGGGT 
ATTCTTGCCC AAGTTTACAA CCCCAACATG CCACCACCCC TGGCTCAGCC ATCAAGCATC 
CTGCCCTACA CCCTCGTCAG TCAGCCCTCG GTCCAGTTAA TCCTGCAGGG GACCCTTCCC 
CTGGCTGGCT GTGGCACAGC ACAGACCCTG GCCCCAGTGC CTAATGTTCC AGCTGTAGCC 
TCAGAGCTGG CTGTTCTAAC CAACAACAGC AGCTCTGAGG AGAGGACCAG CACTCCCAAG 
CCAGCCGCCG AGAAGACCAA GAAGGAAGAG TACTTGAAGA AGCTGCACAT GCAGGAGCGG 
GCTGTGGAGG AGGTGAAGCT GGCCATTAAG CCCTTCTACC AGAAGAGAGA GGTGACCAAG 
GAGGAATACA AGGACATCCT GCGGAAAGCT GTGCAGAAGA TCTGCCACAG CAAGAGCGGC 
GAGATCAACC CTGTGAAGGT GGCCAATCTG GTAAAGGCCT ATGTGGACAA GTATAGGCAC 
ATGCGCAGGC ACAAGAAGAC TGAAGCTGGG GAGGAGCCAC CTGCCCAGGG TGCCGAGACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017659063.1
CDS	116..5158
Translation	MDDDSLDELVAHSPGPNGPPRVVPSELASEAEESGNGHSGDSEDYSGSEHEDNTDGEETEGLSEEEDPEDRSGSEDSEDGIETSVAAVGTQGKLETSSALNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIVEWSRNANSCPVDRTVFKCICIRARLDGKILKKIPVENTRAREDEEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECVTPGAASTHAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRSLTPRVPAKRKRKAGRRKKVLGRKTTRSRSSVKRKSGGLRAKKRQHRVRKTKGRKMKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEELSANLPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILTGQSLLMMSSADVVIHRDGSLSAKRTAPVSLQRNSVTQSRGEDESRRRDSLQPEASPSGSPSNVGLTGSRPQSSGISCGDRSALRCIPAYSVGTPARPESSSTPGSSQAGKLSNGSRPSLKQNNSPQFNGANMQALPLRSASSKISATHSNSSSRNIVLRQSLKTAPRRADISQLPRIPKIRRNGSGSRQDPAPAHGQSVELPSTCISRLTGREGAGQPGRGRAENEPRNRGPQEPGSHTGGSRPPAPSSHGNLAPLGPSRGKGIGSTFESFRINIPGNTAHSSQLSSPGFCNTFRPVDSKVQRKENPSPLFSIKKPKQLKSEIYDPFDPTGSDSSPPTSSPESIGPGLLPSEITRTISINSPKAPAFQAVRCVTSYRVESIFDTEVEPEPQPPGEPMSGMFKFLGKGPAEGASDLEQERLGEVEHPEIEGSIAQTHRPSPQDPWEDEDGMSCTPFFGSEERTVTCVTVMEPDVPQVTTHRIVELRTPSRSRSTSSSRSKKKTKKKRAAREHQRTRSSTRSGSRDRTSRSVSPVVEEHTKRHRAKTKSRRSSSDRASSQERVKRRKTRDRDRERRRGSWSHGRCRRKSRSHSGSPGSSSCERHESRKRKRRRSGSRSRGKDCSSPSNLERARRHKHRERSRSRDKKDSMTWSRERRRWRSRSPSLEHRPRRPRSREKRPRSPEKAPAVKEASPAPTAQEELRQDEDRPAKPSVSEEVSVLPEVVVADLNPPEMPPVLAVPAESVPEDLDYGDSVEAGHVFEDFSSEAIFIQLDDMSSPPSPESTDSSPERDFPPNPVVPPAIQPQDASLAAIQREVSLIHSEDISQPTPQAEGPSDQYLLRQDAVETIAMTPSTMGVVPMGKDSPLLSGRGCEAVRSKETVVQAPLLRARALVKRVTWNLQEAEDGTPALDRVPRTPLQRPQRPREGDWDAEDGALVGVQQAPFSELPPPIHVLQESGLPDADPSQPPGASRAEEVPAVGTLHSAEGILAQVYNPNMPPPLAQPSSILPYTLVSQPSVQLILQGTLPLAGCGTAQTLAPVPNVPAVASELAVLTNNSSSEERTSTPKPAAEKTKKEEYLKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEAGEEPPAQGAET

Target ORF information:

RefSeq Version	XM_017803574.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili PHD and ring finger domains 1 (Phrf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017803574.1

ORF Insert Sequence:

ATGGATGACG ACAGTTTGGA TGAGCTTGTG GCCCACAGCC CTGGGCCAAA TGGACCTCCA 
AGAGTCGTCC CCTCAGAACT GGCCAGTGAA GCTGAAGAAA GTGGCAATGG TCACAGTGGA 
GACTCTGAAG ACTACAGTGG CAGTGAGCAT GAAGACAACA CAGATGGGGA AGAAACAGAG 
GGGTTGTCTG AGGAGGAAGA CCCAGAAGAT AGATCTGGTT CTGAAGATTC AGAAGATGGA 
ATAGAAACAT CAGTGGCAGC AGTGGGTACT CAAGGGAAGC TGGAAACTAG CAGCGCACTC 
AATTCTGATG ATGATGCAGA GAGCTGCCCT ATTTGCCTCA ATGCATTCAG AGACCAGGCT 
GTGGGCACTC CAGAGACCTG TGCCCACTAT TTCTGCTTGG ACTGTATTGT CGAATGGTCT 
AGGAATGCCA ACTCCTGTCC AGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGAGCT 
CGACTTGATG GTAAAATTTT AAAGAAGATT CCAGTGGAAA ACACTAGAGC CCGTGAGGAT 
GAGGAGGCTG AGGAGGAGGA CCCGACCTTC TGTGAGGTGT GTGGTAGGAG TGACCGTGAG 
GACCGGCTCC TGCTCTGTGA TGGCTGTGAT GCTGGGTACC ACATGGAATG TTTAGATCCT 
CCTCTCCAGG AGGTACCTGT GGATGAGTGG TTCTGCCCAG AGTGTGTCAC CCCTGGTGCT 
GCCTCCACTC ATGCTCCTGT GAGTGATGAG GAGGTCTCAC TGTTACTGGC CGACGTGGTA 
CCCACCACCA GCAGGCTGCG GCCTCGTGTG GGCAGGACCC GAGCCATCGC CAGGACACGA 
CAGAGTGAGA GAGTGAGGGC CACTGTAAAC CGGAACAGGA TCTCATCTGC CAGAAGGGTT 
CAGCATGTGC CAAGATACCT CATGTCTTCT CTGCTGGATG AGACTATCGA GGCTGTCGCC 
ACTGGTCTGA GCACTGCTGT GTACCAGCGC TCCCTGACTC CTCGTGTCCC TGCAAAACGG 
AAAAGGAAGG CAGGGAGACG GAAAAAAGTG CTTGGAAGAA AGACGACCCG GTCTAGGTCA 
TCTGTGAAAA GGAAGAGTGG GGGTCTGAGA GCCAAGAAAC GCCAGCACCG TGTGAGGAAG 
ACAAAAGGGA GAAAGATGAA GAATGAGGTT ACAGCTCGTT CTCGAATTGC ACGGACACTG 
GGCCTCCGCA GGCCTGTCCG TGGCACCAGC ATGCCATCAG TGTACAAACC AGTAGACCCC 
TCCCTGGGGC TAATGAGGGC AGATATCGGA GCAGCTTCTC TGTCACTGTT TGGAGACCCC 
TACGAGCTGG ACCCTTTTGA CAGCAATGAA GAACTGTCTG CAAATCTGCC TTCCCCTCTG 
AGTGCCAAGA GGAGAGTGCT GTCCCGCTCG GCCTTGCAGT CTCACCAGCC TGTAGCCAGG 
CCTGTTGCTA TGGGGCTCTC CAGAAGGCAG CTCCCAGCTG TGGCCCCAGA GCCCAGTGTG 
GAGGAGGCTC CTGTTCCTGA TCTGTTGGGG AGCATCCTGA CTGGCCAGAG CCTCCTGATG 
ATGAGCAGCG CTGATGTTGT CATCCACCGG GATGGCTCTC TTAGCGCAAA GAGGACAGCT 
CCTGTTTCTC TTCAACGAAA TTCTGTGACT CAATCCAGAG GAGAAGATGA GTCCAGGCGC 
AGAGACAGCC TACAGCCTGA GGCATCACCC TCAGGGAGCC CATCCAATGT CGGACTCACA 
GGAAGCAGGC CACAGAGCTC AGGGATAAGC TGTGGGGACA GGTCGGCCCT ACGCTGTATC 
CCCGCCTACT CAGTGGGGAC ACCTGCGAGG CCGGAGTCAT CATCAACCCC TGGTTCAAGT 
CAGGCTGGGA AGCTTTCAAA TGGGAGCAGG CCTAGCCTGA AGCAGAATAA CAGCCCCCAG 
TTTAATGGCG CGAACATGCA GGCCTTGCCT CTTCGTTCTG CCTCCTCTAA GATCTCAGCC 
ACTCACTCTA ACTCTTCATC TAGAAATATA GTCCTTAGAC AGTCCCTGAA AACAGCTCCC 
AGGAGAGCCG ACATCTCACA GTTGCCCAGA ATACCAAAGA TCCGTAGGAA TGGCAGTGGC 
AGCCGACAAG ACCCTGCCCC AGCCCATGGG CAGAGCGTGG AGCTCCCCAG CACCTGCATT 
AGCCGCCTGA CAGGAAGGGA GGGTGCTGGA CAGCCAGGGC GAGGCCGAGC TGAGAATGAG 
CCCAGAAACA GGGGCCCTCA GGAGCCTGGT TCACACACGG GTGGCTCTCG GCCTCCTGCC 
CCCAGCTCCC ATGGCAACTT GGCCCCTCTA GGGCCTTCAA GAGGGAAAGG CATTGGGTCA 
ACCTTTGAGA GTTTCAGGAT CAACATCCCC GGGAATACTG CACACAGCAG CCAGCTGTCC 
AGCCCTGGCT TCTGTAACAC ATTCCGGCCA GTGGATAGCA AAGTGCAGAG GAAGGAGAAC 
CCTTCGCCAC TCTTCTCCAT CAAGAAGCCG AAGCAACTCA AGAGTGAAAT CTATGACCCC 
TTTGACCCCA CTGGCTCTGA CTCTAGTCCT CCTACCAGCA GCCCAGAGAG TATTGGCCCA 
GGCCTTCTGC CATCTGAGAT CACGAGAACA ATCTCCATCA ACAGCCCAAA AGCCCCAGCC 
TTCCAGGCTG TGCGCTGTGT TACCTCCTAC AGGGTGGAAA GCATCTTTGA TACTGAGGTG 
GAACCTGAGC CCCAGCCCCC TGGTGAGCCC ATGTCTGGCA TGTTCAAGTT CCTGGGCAAG 
GGGCCTGCTG AGGGAGCCTC TGACTTGGAG CAAGAAAGGC TAGGTGAGGT GGAGCATCCA 
GAAATCGAGG GTTCCATAGC CCAGACACAC AGGCCATCCC CACAAGACCC CTGGGAAGAT 
GAGGATGGAA TGTCCTGTAC ACCCTTCTTT GGTTCTGAGG AGCGGACAGT GACATGTGTG 
ACTGTCATGG AGCCAGATGT TCCACAGGTC ACCACACATA GGATTGTAGA GCTGAGAACC 
CCATCCCGAT CCCGTTCCAC CTCTAGCTCC CGAAGCAAGA AGAAAACCAA GAAGAAGAGG 
GCTGCCAGGG AGCACCAGAG GACACGCTCT AGCACTCGCT CTGGCTCCAG GGACAGGACC 
TCACGTTCGG TATCGCCCGT GGTCGAGGAG CACACCAAGA GGCACAGGGC CAAGACCAAG 
AGCCGGAGGT CTTCCAGCGA CCGTGCCAGC AGCCAGGAGA GAGTGAAGAG GAGGAAGACC 
AGGGACAGGG ACAGGGAGCG GAGACGGGGC TCCTGGAGCC ATGGCAGGTG CCGCAGGAAG 
TCCAGGTCTC ACTCAGGCAG TCCTGGCAGT TCTTCCTGTG AGCGCCATGA AAGCAGGAAG 
CGGAAGAGGC GACGCTCAGG CTCCAGGTCT CGGGGCAAGG ACTGCTCTTC CCCCAGCAAC 
CTAGAGCGGG CCAGGAGACA CAAGCACCGG GAACGGAGCC GGAGCCGAGA CAAGAAAGAC 
AGTATGACCT GGTCCCGGGA GAGGAGGAGG TGGAGGTCTA GATCACCAAG CTTGGAGCAT 
AGGCCACGGC GGCCACGTTC CCGAGAGAAG CGGCCCCGTT CCCCAGAGAA GGCTCCAGCT 
GTGAAGGAAG CTTCCCCAGC CCCCACTGCT CAGGAGGAGC TGAGGCAAGA TGAAGACCGC 
CCGGCTAAAC CTTCAGTCTC CGAGGAAGTG AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCCC CAGAAATGCC CCCTGTCCTG GCAGTGCCAG CTGAATCTGT GCCTGAGGAC 
TTGGACTATG GTGACTCTGT GGAAGCAGGG CACGTCTTTG AGGATTTTTC AAGTGAAGCC 
ATCTTTATCC AGCTTGATGA CATGAGTTCA CCTCCTTCCC CTGAGAGTAC AGACTCCTCC 
CCAGAACGAG ACTTCCCACC AAATCCTGTG GTGCCCCCAG CCATCCAGCC ACAGGATGCC 
AGTCTGGCTG CCATCCAGAG GGAAGTGTCC CTGATCCACA GTGAAGACAT CTCTCAGCCC 
ACACCACAGG CAGAGGGCCC TTCAGACCAG TACCTGCTCA GGCAGGACGC TGTAGAGACC 
ATTGCCATGA CTCCCAGCAC CATGGGTGTG GTACCCATGG GAAAAGACAG CCCCTTGCTG 
AGTGGGAGAG GGTGTGAAGC AGTCAGGTCC AAGGAGACAG TGGTCCAGGC CCCTCTGCTA 
CGGGCCAGGG CCCTTGTAAA AAGAGTCACC TGGAACCTAC AGGAGGCAGA GGATGGTACT 
CCAGCCCTGG ACAGAGTTCC AAGGACACCA CTTCAGAGGC CACAGAGGCC CCGGGAAGGA 
GACTGGGATG CAGAGGACGG GGCCCTCGTA GGAGTTCAGC AGGCACCATT CTCCGAGCTG 
CCCCCTCCCA TCCACGTGCT CCAGGAGTCT GGGTTACCTG ATGCAGACCC CTCTCAGCCC 
CCTGGTGCTT CCAGGGCAGA AGAGGTGCCA GCTGTTGGGA CTCTGCACTC AGCAGAGGGT 
ATTCTTGCCC AAGTTTACAA CCCCAACATG CCACCACCCC TGGCTCAGCC ATCAAGCATC 
CTGCCCTACA CCCTCGTCAG TCAGCCCTCG GTCCAGTTAA TCCTGCAGGG GACCCTTCCC 
CTGGCTGGCT GTGGCACAGC ACAGACCCTG GCCCCAGTGC CTAATGTTCC AGCTGTAGCC 
TCAGAGCTGG CTGTTCTAAC CAACAACAGC AGCTCTGAGG AGAGGACCAG CACTCCCAAG 
CCAGCCGCCG AGAAGACCAA GAAGGAAGAG TACTTGAAGA AGCTGCACAT GCAGGAGCGG 
GCTGTGGAGG AGGTGAAGCT GGCCATTAAG CCCTTCTACC AGAAGAGAGA GGTGACCAAG 
GAGGAATACA AGGACATCCT GCGGAAAGCT GTGCAGAAGA TCTGCCACAG CAAGAGCGGC 
GAGATCAACC CTGTGAAGGT GGCCAATCTG GTAAAGGCCT ATGTGGACAA GTATAGGCAC 
ATGCGCAGGC ACAAGAAGAC TGAAGCTGGG GAGGAGCCAC CTGCCCAGGG TGCCGAGACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Phrf1 cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム