Bmp1 cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bmp1
Entrez Gene ID	103748138
Full Name	bone morphogenetic protein 1
Gene Type	protein-coding
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017802522.1	XP_017658011.1	bone morphogenetic protein 1
XM_029554602.1	XP_029410462.1	bone morphogenetic protein 1

Pathways from BioSystems
Gene Ontology

The following Bmp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bmp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONa137774	XM_029554602.1 Latest version!	Nannospalax galili bone morphogenetic protein 1 (Bmp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ONa37824	XM_017802522.1 Latest version!	Nannospalax galili bone morphogenetic protein 1 (Bmp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bmp1 ( XM_029554602.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029410462.1 Nannospalax galili bone morphogenetic protein 1 (Bmp1), mRNA.

Price & Availability↑

CloneID	ONa137774
Clone ID Related Accession (Same CDS sequence)	XM_029554602.1
Accession Version	XM_029554602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	bone morphogenetic protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008330588.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCACGGAG GCTCGGAGAC CGCGCTGACA GCGTGTCCGC GCCGTGAGGC ACTCGGCCTG 
GCACCCCGGG CACCGGCCGC TAGGGGGCCC CTCGAGGGAG AGGCGGCCGC CGGGCATCCC 
CGGGGCTCGC GCCCCCTCCC CGGCCCCGGC CCCAGGCAGC CGATCCCCTC CGGAGCGGAC 
TGCGCGCCTC CCGCCCTCCC CGCCCGGCTG GCCCGCTTCC CGGCCCGGCC GAGCACCGTC 
CCGGCCCCGA GGAGGGCCGA GCTGGGCGAA AACCCGCCCT CCAGCGAGCT CATTTCCCTA 
AAAAGGGAGG GGGGAGAGCG GGAGGGCGGC GAGCAAAGAA AGGCTGCGAG CGCAACGAGG 
GTGAGGGGAC CCGGCTGCCG AGGACCAGAG CGAGCCGGGC CGAGCGGGGG GCGGGAGACA 
GGAAGCGGGA AGGGAAGAGG TAGCGACGCG GGGGGAGCGG GCGGCCAGCG CCGGGAGCAC 
CTCCCCTTCG CCTCCAGGGC TCCGCTTCCC TGTCGCCCCG CTCCCGCGTC CGGGCGCGAG 
CCCGGCGTGG CCCGCCCGCC GCTGCCGCCG CCGCTGTCGC TGCCGCTGCT GCTGCTGCTG 
CTGCTCCCGC GCCCCGGGCG GCCGCTGGAC TTGGCCGACT ATACCTACGA CCTGGGCGAG 
GAGGATGCCC CCGAGCTCCT CAACTACAAA GACCCCTGCA AGGCGGCTGC CTTTCTTGGG 
GACATTGCCC TGGATGAGGA AGACTTGAGG GCCTTCCAGG TGCAGGCTAC AGTTCTCAGA 
CAGCAAACAG CCCGAAGATC ATCCATCAGA GCTGCAGGAA ACTTTTCTGC CCCTGGTGGC 
CAGAGCGCTA GCGGGCAGCC ACAGAGGGAA ACCCAGCAGA GATGGAGAGG CAGACCTCGG 
AGCCGGAGTC GTCGGGCAGC AACATCCAGA CCTGAGCGTG TGTGGCCTGA TGGGGTCATC 
CCTTTTGTCA TTGGAGGGAA CTTCACTGGC AGCCAGAGGG CAGTCTTCCG GCAAGCCATG 
AGGCACTGGG AGAAGCACAC TTGTGTCACC TTCTTGGAGC GCACAGACGA GGACAGCTAT 
ATTGTATTCA CCTATCGACC CTGCGGGTGC TGCTCCTACG TCGGTCGCCG TGGTGGGGGC 
CCCCAGGCCA TCTCCATCGG CAAGAACTGT GACAAGTTCG GCATCGTGGT CCATGAGCTG 
GGCCACGTCA TTGGCTTCTG GCACGAGCAT ACGCGGCCTG ACCGGGACCG CCACGTCTCT 
ATAATACGAG AGAATATACA GCCAGGGCAG GAGTATAATT TCCTGAAGAT GGAGGTTCAG 
GAGGTGGAGT CCTTGGGAGA GACCTATGAC TTTGACAGTA TCATGCACTA TGCCCGGAAC 
ACGTTCTCCA GGGGCATCTT CCTGGACACC ATTGTCCCCA AGTATGAGGT GAATGGGGTG 
AAGCCTTCCA TCGGCCAAAG GACCCGACTT AGCAAGGGGG ACATCACCCA GGCCCGAAAG 
CTCTACAAGT GCCCAGCCTG CGGTGAGACC CTCCAAGACA GCACGGGCAA CTTCTCCTCC 
CCTGAGTACC CCAACGGCTA CTCTGCCCAC ATGCACTGTG TATGGCGCAT CTCGGTTACA 
CCTGGGGAGA AGATCATCCT GAACTTCACA TCCATGGACC TATACCGTAG CCGCCTATGC 
TGGTATGATT ACGTGGAGGT ACGCGATGGC TTCTGGAGGA AGGCGCCCCT CCGAGGACGG 
TTCTGCGGGG GCAAACTCCC TGAACCCATC GTCTCCACCG ACAGCCGCCT CTGGGTGGAA 
TTTCGCAGCA GCAGCAATTG GGTCGGAAAG GGCTTCTTTG CAGTCTATGA AGCTATCTGT 
GGTGGTGATG TGAAAAAAGA TAACGGCCAC ATCCAGTCAC CCAATTACCC AGATGACTAC 
CGGCCCAGCA AAGTCTGCAT CTGGCGGATC CAGGTGTCCG AAGGCTTCCA CGTGGGCCTC 
ACGTTCCAGT CCTTTGAGAT TGAGCGTCAT GACAGCTGTG CCTACGACTA CTTGGAAGTT 
CGTGATGGGC ACAGTGAGAG CAGCAACCTC ATTGGGCGCT ACTGCGGCTA TGAGAAGCCC 
GATGACATCA AAAGCACATC TAGCCGTCTC TGGCTCAAGT TCGTCTCCGA TGGATCCATT 
AACAAAGCTG GCTTCGCAGT CAATTTTTTC AAAGAGGTGG ATGAATGCTC ACGGCCCAAC 
CGTGGAGGCT GTGAGCAGCG GTGCCTGAAC ACCCTGGGCA GCTACAAGTG CAGCTGTGAC 
CCGGGGTACG AGCTGGCCCC CGACAAGCGC CGATGTGAGG CTGCCTGTGG TGGATTCCTC 
ACCAAGCTCA ACGGCTCCAT CACCAGCCCA GGCTGGCCTA AGGAATACCC TCCCAACAAA 
AACTGCATCT GGCAGTTGGT GGCCCCTACC CAGTACCGTA TCTCCTTGCA GTTTGACTTC 
TTTGAGACGG AGGGCAATGA CGTATGCAAG TATGACTTTG TGGAGGTACG CAGTGGACTC 
ACGGCTGACT CTAAGCTGCA TGGCAAGTTC TGTGGCTCTG AGAAACCAGA AGTCATCACT 
TCCCAATACA ACAACATGCG TGTGGAATTC AAGTCCGACA ATACTGTATC CAAAAAGGGC 
TTCAAGGCCC ACTTCTTCTC AGACAAGGAT GAGTGTTCCA AGGACAATGG TGGCTGCCAG 
CAAGACTGCG TGAACACGTT TGGCAGCTAT GAGTGTCAGT GTCGCAGCGG CTTCGTCCTC 
CATGACAACA AACATGACTG CAAGGAAGCC GGCTGTGACC ACAAGGTGAC ATCTACCAGT 
GGCACCATCA CCAGCCCCAA CTGGCCTGAC AAATACCCCA GCAAGAAGGA ATGCACGTGG 
GCTATCTCCA GCACCCCTGG GCATCGGGTC AAGCTGACCT TCATGGAGAT GGACATTGAG 
TCTCAGCCCG AGTGTGCTTA TGACCACCTT GAGGTGTTTG ATGGGCGTGA CACCAAGGCA 
CCAGTCCTCG GCCGTTTCTG TGGCAGCAAG AAGCCAGAAC CTGTCCTGGC TACAGGCAGT 
CGCATGTTCC TGCGCTTCTA CTCGGACAAC TCTGTACAGA GGAAAGGCTT CCAGGCATCT 
CATTCCACAG AGTGTGGAGG CCAAGTTCGA GCAGATGTGA AGACCAAAGA CCTTTATTCC 
CATGCCCAGT TTGGTGACAA CAACTACCCT GGAGGGGTGG ACTGCGAGTG GGTCATTGTG 
GCTGAGGAGG GCTATGGTGT AGAACTGGTG TTCCAGACCT TCGAGGTGGA GGAAGAGACC 
GACTGTGGCT ATGACTACAT GGAGCTCTTT GATGGCTACG ACAGCACAGC CCCAAGACTG 
GGGCGCTACT GTGGCTCTGG GCCTCCTGAG GAAGTGTACT CGGCTGGAGA TTCTGTCCTG 
GTGAAGTTCC ATTCTGATGA CACCATCACC AAGAAAGGCT TCCACCTGCG GTACACAAGC 
ACCAAGTTTC AGGACACACT CCACAGCAGG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029410462.1
CDS	1..3516
Translation	MHGGSETALTACPRREALGLAPRAPAARGPLEGEAAAGHPRGSRPLPGPGPRQPIPSGADCAPPALPARLARFPARPSTVPAPRRAELGENPPSSELISLKREGGEREGGEQRKAASATRVRGPGCRGPERAGPSGGRETGSGKGRGSDAGGAGGQRREHLPFASRAPLPCRPAPASGREPGVARPPLPPPLSLPLLLLLLLPRPGRPLDLADYTYDLGEEDAPELLNYKDPCKAAAFLGDIALDEEDLRAFQVQATVLRQQTARRSSIRAAGNFSAPGGQSASGQPQRETQQRWRGRPRSRSRRAATSRPERVWPDGVIPFVIGGNFTGSQRAVFRQAMRHWEKHTCVTFLERTDEDSYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDRHVSIIRENIQPGQEYNFLKMEVQEVESLGETYDFDSIMHYARNTFSRGIFLDTIVPKYEVNGVKPSIGQRTRLSKGDITQARKLYKCPACGETLQDSTGNFSSPEYPNGYSAHMHCVWRISVTPGEKIILNFTSMDLYRSRLCWYDYVEVRDGFWRKAPLRGRFCGGKLPEPIVSTDSRLWVEFRSSSNWVGKGFFAVYEAICGGDVKKDNGHIQSPNYPDDYRPSKVCIWRIQVSEGFHVGLTFQSFEIERHDSCAYDYLEVRDGHSESSNLIGRYCGYEKPDDIKSTSSRLWLKFVSDGSINKAGFAVNFFKEVDECSRPNRGGCEQRCLNTLGSYKCSCDPGYELAPDKRRCEAACGGFLTKLNGSITSPGWPKEYPPNKNCIWQLVAPTQYRISLQFDFFETEGNDVCKYDFVEVRSGLTADSKLHGKFCGSEKPEVITSQYNNMRVEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQQDCVNTFGSYECQCRSGFVLHDNKHDCKEAGCDHKVTSTSGTITSPNWPDKYPSKKECTWAISSTPGHRVKLTFMEMDIESQPECAYDHLEVFDGRDTKAPVLGRFCGSKKPEPVLATGSRMFLRFYSDNSVQRKGFQASHSTECGGQVRADVKTKDLYSHAQFGDNNYPGGVDCEWVIVAEEGYGVELVFQTFEVEEETDCGYDYMELFDGYDSTAPRLGRYCGSGPPEEVYSAGDSVLVKFHSDDTITKKGFHLRYTSTKFQDTLHSRK

Target ORF information:

RefSeq Version	XM_029554602.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili bone morphogenetic protein 1 (Bmp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029554602.1

ORF Insert Sequence:

ATGCACGGAG GCTCGGAGAC CGCGCTGACA GCGTGTCCGC GCCGTGAGGC ACTCGGCCTG 
GCACCCCGGG CACCGGCCGC TAGGGGGCCC CTCGAGGGAG AGGCGGCCGC CGGGCATCCC 
CGGGGCTCGC GCCCCCTCCC CGGCCCCGGC CCCAGGCAGC CGATCCCCTC CGGAGCGGAC 
TGCGCGCCTC CCGCCCTCCC CGCCCGGCTG GCCCGCTTCC CGGCCCGGCC GAGCACCGTC 
CCGGCCCCGA GGAGGGCCGA GCTGGGCGAA AACCCGCCCT CCAGCGAGCT CATTTCCCTA 
AAAAGGGAGG GGGGAGAGCG GGAGGGCGGC GAGCAAAGAA AGGCTGCGAG CGCAACGAGG 
GTGAGGGGAC CCGGCTGCCG AGGACCAGAG CGAGCCGGGC CGAGCGGGGG GCGGGAGACA 
GGAAGCGGGA AGGGAAGAGG TAGCGACGCG GGGGGAGCGG GCGGCCAGCG CCGGGAGCAC 
CTCCCCTTCG CCTCCAGGGC TCCGCTTCCC TGTCGCCCCG CTCCCGCGTC CGGGCGCGAG 
CCCGGCGTGG CCCGCCCGCC GCTGCCGCCG CCGCTGTCGC TGCCGCTGCT GCTGCTGCTG 
CTGCTCCCGC GCCCCGGGCG GCCGCTGGAC TTGGCCGACT ATACCTACGA CCTGGGCGAG 
GAGGATGCCC CCGAGCTCCT CAACTACAAA GACCCCTGCA AGGCGGCTGC CTTTCTTGGG 
GACATTGCCC TGGATGAGGA AGACTTGAGG GCCTTCCAGG TGCAGGCTAC AGTTCTCAGA 
CAGCAAACAG CCCGAAGATC ATCCATCAGA GCTGCAGGAA ACTTTTCTGC CCCTGGTGGC 
CAGAGCGCTA GCGGGCAGCC ACAGAGGGAA ACCCAGCAGA GATGGAGAGG CAGACCTCGG 
AGCCGGAGTC GTCGGGCAGC AACATCCAGA CCTGAGCGTG TGTGGCCTGA TGGGGTCATC 
CCTTTTGTCA TTGGAGGGAA CTTCACTGGC AGCCAGAGGG CAGTCTTCCG GCAAGCCATG 
AGGCACTGGG AGAAGCACAC TTGTGTCACC TTCTTGGAGC GCACAGACGA GGACAGCTAT 
ATTGTATTCA CCTATCGACC CTGCGGGTGC TGCTCCTACG TCGGTCGCCG TGGTGGGGGC 
CCCCAGGCCA TCTCCATCGG CAAGAACTGT GACAAGTTCG GCATCGTGGT CCATGAGCTG 
GGCCACGTCA TTGGCTTCTG GCACGAGCAT ACGCGGCCTG ACCGGGACCG CCACGTCTCT 
ATAATACGAG AGAATATACA GCCAGGGCAG GAGTATAATT TCCTGAAGAT GGAGGTTCAG 
GAGGTGGAGT CCTTGGGAGA GACCTATGAC TTTGACAGTA TCATGCACTA TGCCCGGAAC 
ACGTTCTCCA GGGGCATCTT CCTGGACACC ATTGTCCCCA AGTATGAGGT GAATGGGGTG 
AAGCCTTCCA TCGGCCAAAG GACCCGACTT AGCAAGGGGG ACATCACCCA GGCCCGAAAG 
CTCTACAAGT GCCCAGCCTG CGGTGAGACC CTCCAAGACA GCACGGGCAA CTTCTCCTCC 
CCTGAGTACC CCAACGGCTA CTCTGCCCAC ATGCACTGTG TATGGCGCAT CTCGGTTACA 
CCTGGGGAGA AGATCATCCT GAACTTCACA TCCATGGACC TATACCGTAG CCGCCTATGC 
TGGTATGATT ACGTGGAGGT ACGCGATGGC TTCTGGAGGA AGGCGCCCCT CCGAGGACGG 
TTCTGCGGGG GCAAACTCCC TGAACCCATC GTCTCCACCG ACAGCCGCCT CTGGGTGGAA 
TTTCGCAGCA GCAGCAATTG GGTCGGAAAG GGCTTCTTTG CAGTCTATGA AGCTATCTGT 
GGTGGTGATG TGAAAAAAGA TAACGGCCAC ATCCAGTCAC CCAATTACCC AGATGACTAC 
CGGCCCAGCA AAGTCTGCAT CTGGCGGATC CAGGTGTCCG AAGGCTTCCA CGTGGGCCTC 
ACGTTCCAGT CCTTTGAGAT TGAGCGTCAT GACAGCTGTG CCTACGACTA CTTGGAAGTT 
CGTGATGGGC ACAGTGAGAG CAGCAACCTC ATTGGGCGCT ACTGCGGCTA TGAGAAGCCC 
GATGACATCA AAAGCACATC TAGCCGTCTC TGGCTCAAGT TCGTCTCCGA TGGATCCATT 
AACAAAGCTG GCTTCGCAGT CAATTTTTTC AAAGAGGTGG ATGAATGCTC ACGGCCCAAC 
CGTGGAGGCT GTGAGCAGCG GTGCCTGAAC ACCCTGGGCA GCTACAAGTG CAGCTGTGAC 
CCGGGGTACG AGCTGGCCCC CGACAAGCGC CGATGTGAGG CTGCCTGTGG TGGATTCCTC 
ACCAAGCTCA ACGGCTCCAT CACCAGCCCA GGCTGGCCTA AGGAATACCC TCCCAACAAA 
AACTGCATCT GGCAGTTGGT GGCCCCTACC CAGTACCGTA TCTCCTTGCA GTTTGACTTC 
TTTGAGACGG AGGGCAATGA CGTATGCAAG TATGACTTTG TGGAGGTACG CAGTGGACTC 
ACGGCTGACT CTAAGCTGCA TGGCAAGTTC TGTGGCTCTG AGAAACCAGA AGTCATCACT 
TCCCAATACA ACAACATGCG TGTGGAATTC AAGTCCGACA ATACTGTATC CAAAAAGGGC 
TTCAAGGCCC ACTTCTTCTC AGACAAGGAT GAGTGTTCCA AGGACAATGG TGGCTGCCAG 
CAAGACTGCG TGAACACGTT TGGCAGCTAT GAGTGTCAGT GTCGCAGCGG CTTCGTCCTC 
CATGACAACA AACATGACTG CAAGGAAGCC GGCTGTGACC ACAAGGTGAC ATCTACCAGT 
GGCACCATCA CCAGCCCCAA CTGGCCTGAC AAATACCCCA GCAAGAAGGA ATGCACGTGG 
GCTATCTCCA GCACCCCTGG GCATCGGGTC AAGCTGACCT TCATGGAGAT GGACATTGAG 
TCTCAGCCCG AGTGTGCTTA TGACCACCTT GAGGTGTTTG ATGGGCGTGA CACCAAGGCA 
CCAGTCCTCG GCCGTTTCTG TGGCAGCAAG AAGCCAGAAC CTGTCCTGGC TACAGGCAGT 
CGCATGTTCC TGCGCTTCTA CTCGGACAAC TCTGTACAGA GGAAAGGCTT CCAGGCATCT 
CATTCCACAG AGTGTGGAGG CCAAGTTCGA GCAGATGTGA AGACCAAAGA CCTTTATTCC 
CATGCCCAGT TTGGTGACAA CAACTACCCT GGAGGGGTGG ACTGCGAGTG GGTCATTGTG 
GCTGAGGAGG GCTATGGTGT AGAACTGGTG TTCCAGACCT TCGAGGTGGA GGAAGAGACC 
GACTGTGGCT ATGACTACAT GGAGCTCTTT GATGGCTACG ACAGCACAGC CCCAAGACTG 
GGGCGCTACT GTGGCTCTGG GCCTCCTGAG GAAGTGTACT CGGCTGGAGA TTCTGTCCTG 
GTGAAGTTCC ATTCTGATGA CACCATCACC AAGAAAGGCT TCCACCTGCG GTACACAAGC 
ACCAAGTTTC AGGACACACT CCACAGCAGG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bmp1 ( XM_017802522.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017658011.1 Nannospalax galili bone morphogenetic protein 1 (Bmp1), mRNA.

Price & Availability↑

CloneID	ONa37824
Clone ID Related Accession (Same CDS sequence)	XM_017802522.1
Accession Version	XM_017802522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	bone morphogenetic protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008330588.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 16% of CDS bases ##RefSeq-Attributes-END##

ATGGAAGGGG AGCAGGGGGC CTTGCCACGC GTCCTTTCTG GGGGATTTGG TCTGCCGCAG 
GTCCTGGGAC GCAGAGGCTC GGAGACCGCG CTGACAGCGT GTCCGCGCCG TGAGGCACTC 
GGCCTGGCAC CCCGGGCACC GGCCGCTAGG GGGCCCCTCG AGGGAGAGGC GGCCGCCGGG 
CATCCCCGGG GCTCGCGCCC CCTCCCCGGC CCCGGCCCCA GGCAGCCGAT CCCCTCCGGA 
GCGGACTGCG CGCCTCCCGC CCTCCCCGCC CGGCTGGCCC GCTTCCCGGC CCGGCCGAGC 
ACCGTCCCGG CCCCGAGGAG GGCCGAGCTG GGCGAAAACC CGCCCTCCAG CGAGCTCATT 
TCCCTAAAAA GGGAGGGGGG AGAGCGGGAG GGCGGCGAGC AAAGAAAGGC TGCGAGCGCA 
ACGAGGGTGA GGGGACCCGG CTGCCGAGGA CCAGAGCGAG CCGGGCCGAG CGGGGGGCGG 
GAGACAGGAA GCGGGAAGGG AAGAGGTAGC GACGCGGGGG GAGCGGGCGG CCAGCGCCGG 
GAGCACCTCC CCTTCGCCTC CAGGGCTCCG CTTCCCTGTC GCCCCGCTCC CGCGTCCGGG 
CGCGAGCCCG GCGTGGCCCG CCCGCCGCTG CCGCCGCCGC TGTCGCTGCC GCTGCTGCTG 
CTGCTGCTGC TCCCGCGCCC CGGGCGGCCG CTGGACTTGG CCGACTATAC CTACGACCTG 
GGCGAGGAGG ATGCCCCCGA GCTCCTCAAC TACAAAGACC CCTGCAAGGC GGCTGCCTTT 
CTTGGGGACA TTGCCCTGGA TGAGGAAGAC TTGAGGGCCT TCCAGGTGCA GGCTACAGTT 
CTCAGACAGC AAACAGCCCG AAGATCATCC ATCAGAGCTG CAGGAAACTT TTCTGCCCCT 
GGTGGCCAGA GCGCTAGCGG GCAGCCACAG AGGGAAACCC AGCAGAGATG GAGAGGCAGA 
CCTCGGAGCC GGAGTCGTCG GGCAGCAACA TCCAGACCTG AGCGTGTGTG GCCTGATGGG 
GTCATCCCTT TTGTCATTGG AGGGAACTTC ACTGGCAGCC AGAGGGCAGT CTTCCGGCAA 
GCCATGAGGC ACTGGGAGAA GCACACTTGT GTCACCTTCT TGGAGCGCAC AGACGAGGAC 
AGCTATATTG TATTCACCTA TCGACCCTGC GGGTGCTGCT CCTACGTCGG TCGCCGTGGT 
GGGGGCCCCC AGGCCATCTC CATCGGCAAG AACTGTGACA AGTTCGGCAT CGTGGTCCAT 
GAGCTGGGCC ACGTCATTGG CTTCTGGCAC GAGCATACGC GGCCTGACCG GGACCGCCAC 
GTCTCTATAA TACGAGAGAA TATACAGCCA GGGCAGGAGT ATAATTTCCT GAAGATGGAG 
GTTCAGGAGG TGGAGTCCTT GGGAGAGACC TATGACTTTG ACAGTATCAT GCACTATGCC 
CGGAACACGT TCTCCAGGGG CATCTTCCTG GACACCATTG TCCCCAAGTA TGAGGTGAAT 
GGGGTGAAGC CTTCCATCGG CCAAAGGACC CGACTTAGCA AGGGGGACAT CACCCAGGCC 
CGAAAGCTCT ACAAGTGCCC AGCCTGCGGT GAGACCCTCC AAGACAGCAC GGGCAACTTC 
TCCTCCCCTG AGTACCCCAA CGGCTACTCT GCCCACATGC ACTGTGTATG GCGCATCTCG 
GTTACACCTG GGGAGAAGAT CATCCTGAAC TTCACATCCA TGGACCTATA CCGTAGCCGC 
CTATGCTGGT ATGATTACGT GGAGGTACGC GATGGCTTCT GGAGGAAGGC GCCCCTCCGA 
GGACGGTTCT GCGGGGGCAA ACTCCCTGAA CCCATCGTCT CCACCGACAG CCGCCTCTGG 
GTGGAATTTC GCAGCAGCAG CAATTGGGTC GGAAAGGGCT TCTTTGCAGT CTATGAAGCT 
ATCTGTGGTG GTGATGTGAA AAAAGATAAC GGCCACATCC AGTCACCCAA TTACCCAGAT 
GACTACCGGC CCAGCAAAGT CTGCATCTGG CGGATCCAGG TGTCCGAAGG CTTCCACGTG 
GGCCTCACGT TCCAGTCCTT TGAGATTGAG CGTCATGACA GCTGTGCCTA CGACTACTTG 
GAAGTTCGTG ATGGGCACAG TGAGAGCAGC AACCTCATTG GGCGCTACTG CGGCTATGAG 
AAGCCCGATG ACATCAAAAG CACATCTAGC CGTCTCTGGC TCAAGTTCGT CTCCGATGGA 
TCCATTAACA AAGCTGGCTT CGCAGTCAAT TTTTTCAAAG AGGTGGATGA ATGCTCACGG 
CCCAACCGTG GAGGCTGTGA GCAGCGGTGC CTGAACACCC TGGGCAGCTA CAAGTGCAGC 
TGTGACCCGG GGTACGAGCT GGCCCCCGAC AAGCGCCGAT GTGAGGCTGC CTGTGGTGGA 
TTCCTCACCA AGCTCAACGG CTCCATCACC AGCCCAGGCT GGCCTAAGGA ATACCCTCCC 
AACAAAAACT GCATCTGGCA GTTGGTGGCC CCTACCCAGT ACCGTATCTC CTTGCAGTTT 
GACTTCTTTG AGACGGAGGG CAATGACGTA TGCAAGTATG ACTTTGTGGA GGTACGCAGT 
GGACTCACGG CTGACTCTAA GCTGCATGGC AAGTTCTGTG GCTCTGAGAA ACCAGAAGTC 
ATCACTTCCC AATACAACAA CATGCGTGTG GAATTCAAGT CCGACAATAC TGTATCCAAA 
AAGGGCTTCA AGGCCCACTT CTTCTCAGAC AAGGATGAGT GTTCCAAGGA CAATGGTGGC 
TGCCAGCAAG ACTGCGTGAA CACGTTTGGC AGCTATGAGT GTCAGTGTCG CAGCGGCTTC 
GTCCTCCATG ACAACAAACA TGACTGCAAG GAAGCCGGCT GTGACCACAA GGTGACATCT 
ACCAGTGGCA CCATCACCAG CCCCAACTGG CCTGACAAAT ACCCCAGCAA GAAGGAATGC 
ACGTGGGCTA TCTCCAGCAC CCCTGGGCAT CGGGTCAAGC TGACCTTCAT GGAGATGGAC 
ATTGAGTCTC AGCCCGAGTG TGCTTATGAC CACCTTGAGG TGTTTGATGG GCGTGACACC 
AAGGCACCAG TCCTCGGCCG TTTCTGTGGC AGCAAGAAGC CAGAACCTGT CCTGGCTACA 
GGCAGTCGCA TGTTCCTGCG CTTCTACTCG GACAACTCTG TACAGAGGAA AGGCTTCCAG 
GCATCTCATT CCACAGAGTG TGGAGGCCAA GTTCGAGCAG ATGTGAAGAC CAAAGACCTT 
TATTCCCATG CCCAGTTTGG TGACAACAAC TACCCTGGAG GGGTGGACTG CGAGTGGGTC 
ATTGTGGCTG AGGAGGGCTA TGGTGTAGAA CTGGTGTTCC AGACCTTCGA GGTGGAGGAA 
GAGACCGACT GTGGCTATGA CTACATGGAG CTCTTTGATG GCTACGACAG CACAGCCCCA 
AGACTGGGGC GCTACTGTGG CTCTGGGCCT CCTGAGGAAG TGTACTCGGC TGGAGATTCT 
GTCCTGGTGA AGTTCCATTC TGATGACACC ATCACCAAGA AAGGCTTCCA CCTGCGGTAC 
ACAAGCACCA AGTTTCAGGA CACACTCCAC AGCAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017658011.1
CDS	1..3582
Translation	MEGEQGALPRVLSGGFGLPQVLGRRGSETALTACPRREALGLAPRAPAARGPLEGEAAAGHPRGSRPLPGPGPRQPIPSGADCAPPALPARLARFPARPSTVPAPRRAELGENPPSSELISLKREGGEREGGEQRKAASATRVRGPGCRGPERAGPSGGRETGSGKGRGSDAGGAGGQRREHLPFASRAPLPCRPAPASGREPGVARPPLPPPLSLPLLLLLLLPRPGRPLDLADYTYDLGEEDAPELLNYKDPCKAAAFLGDIALDEEDLRAFQVQATVLRQQTARRSSIRAAGNFSAPGGQSASGQPQRETQQRWRGRPRSRSRRAATSRPERVWPDGVIPFVIGGNFTGSQRAVFRQAMRHWEKHTCVTFLERTDEDSYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDRHVSIIRENIQPGQEYNFLKMEVQEVESLGETYDFDSIMHYARNTFSRGIFLDTIVPKYEVNGVKPSIGQRTRLSKGDITQARKLYKCPACGETLQDSTGNFSSPEYPNGYSAHMHCVWRISVTPGEKIILNFTSMDLYRSRLCWYDYVEVRDGFWRKAPLRGRFCGGKLPEPIVSTDSRLWVEFRSSSNWVGKGFFAVYEAICGGDVKKDNGHIQSPNYPDDYRPSKVCIWRIQVSEGFHVGLTFQSFEIERHDSCAYDYLEVRDGHSESSNLIGRYCGYEKPDDIKSTSSRLWLKFVSDGSINKAGFAVNFFKEVDECSRPNRGGCEQRCLNTLGSYKCSCDPGYELAPDKRRCEAACGGFLTKLNGSITSPGWPKEYPPNKNCIWQLVAPTQYRISLQFDFFETEGNDVCKYDFVEVRSGLTADSKLHGKFCGSEKPEVITSQYNNMRVEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQQDCVNTFGSYECQCRSGFVLHDNKHDCKEAGCDHKVTSTSGTITSPNWPDKYPSKKECTWAISSTPGHRVKLTFMEMDIESQPECAYDHLEVFDGRDTKAPVLGRFCGSKKPEPVLATGSRMFLRFYSDNSVQRKGFQASHSTECGGQVRADVKTKDLYSHAQFGDNNYPGGVDCEWVIVAEEGYGVELVFQTFEVEEETDCGYDYMELFDGYDSTAPRLGRYCGSGPPEEVYSAGDSVLVKFHSDDTITKKGFHLRYTSTKFQDTLHSRK

Target ORF information:

RefSeq Version	XM_017802522.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili bone morphogenetic protein 1 (Bmp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017802522.1

ORF Insert Sequence:

ATGGAAGGGG AGCAGGGGGC CTTGCCACGC GTCCTTTCTG GGGGATTTGG TCTGCCGCAG 
GTCCTGGGAC GCAGAGGCTC GGAGACCGCG CTGACAGCGT GTCCGCGCCG TGAGGCACTC 
GGCCTGGCAC CCCGGGCACC GGCCGCTAGG GGGCCCCTCG AGGGAGAGGC GGCCGCCGGG 
CATCCCCGGG GCTCGCGCCC CCTCCCCGGC CCCGGCCCCA GGCAGCCGAT CCCCTCCGGA 
GCGGACTGCG CGCCTCCCGC CCTCCCCGCC CGGCTGGCCC GCTTCCCGGC CCGGCCGAGC 
ACCGTCCCGG CCCCGAGGAG GGCCGAGCTG GGCGAAAACC CGCCCTCCAG CGAGCTCATT 
TCCCTAAAAA GGGAGGGGGG AGAGCGGGAG GGCGGCGAGC AAAGAAAGGC TGCGAGCGCA 
ACGAGGGTGA GGGGACCCGG CTGCCGAGGA CCAGAGCGAG CCGGGCCGAG CGGGGGGCGG 
GAGACAGGAA GCGGGAAGGG AAGAGGTAGC GACGCGGGGG GAGCGGGCGG CCAGCGCCGG 
GAGCACCTCC CCTTCGCCTC CAGGGCTCCG CTTCCCTGTC GCCCCGCTCC CGCGTCCGGG 
CGCGAGCCCG GCGTGGCCCG CCCGCCGCTG CCGCCGCCGC TGTCGCTGCC GCTGCTGCTG 
CTGCTGCTGC TCCCGCGCCC CGGGCGGCCG CTGGACTTGG CCGACTATAC CTACGACCTG 
GGCGAGGAGG ATGCCCCCGA GCTCCTCAAC TACAAAGACC CCTGCAAGGC GGCTGCCTTT 
CTTGGGGACA TTGCCCTGGA TGAGGAAGAC TTGAGGGCCT TCCAGGTGCA GGCTACAGTT 
CTCAGACAGC AAACAGCCCG AAGATCATCC ATCAGAGCTG CAGGAAACTT TTCTGCCCCT 
GGTGGCCAGA GCGCTAGCGG GCAGCCACAG AGGGAAACCC AGCAGAGATG GAGAGGCAGA 
CCTCGGAGCC GGAGTCGTCG GGCAGCAACA TCCAGACCTG AGCGTGTGTG GCCTGATGGG 
GTCATCCCTT TTGTCATTGG AGGGAACTTC ACTGGCAGCC AGAGGGCAGT CTTCCGGCAA 
GCCATGAGGC ACTGGGAGAA GCACACTTGT GTCACCTTCT TGGAGCGCAC AGACGAGGAC 
AGCTATATTG TATTCACCTA TCGACCCTGC GGGTGCTGCT CCTACGTCGG TCGCCGTGGT 
GGGGGCCCCC AGGCCATCTC CATCGGCAAG AACTGTGACA AGTTCGGCAT CGTGGTCCAT 
GAGCTGGGCC ACGTCATTGG CTTCTGGCAC GAGCATACGC GGCCTGACCG GGACCGCCAC 
GTCTCTATAA TACGAGAGAA TATACAGCCA GGGCAGGAGT ATAATTTCCT GAAGATGGAG 
GTTCAGGAGG TGGAGTCCTT GGGAGAGACC TATGACTTTG ACAGTATCAT GCACTATGCC 
CGGAACACGT TCTCCAGGGG CATCTTCCTG GACACCATTG TCCCCAAGTA TGAGGTGAAT 
GGGGTGAAGC CTTCCATCGG CCAAAGGACC CGACTTAGCA AGGGGGACAT CACCCAGGCC 
CGAAAGCTCT ACAAGTGCCC AGCCTGCGGT GAGACCCTCC AAGACAGCAC GGGCAACTTC 
TCCTCCCCTG AGTACCCCAA CGGCTACTCT GCCCACATGC ACTGTGTATG GCGCATCTCG 
GTTACACCTG GGGAGAAGAT CATCCTGAAC TTCACATCCA TGGACCTATA CCGTAGCCGC 
CTATGCTGGT ATGATTACGT GGAGGTACGC GATGGCTTCT GGAGGAAGGC GCCCCTCCGA 
GGACGGTTCT GCGGGGGCAA ACTCCCTGAA CCCATCGTCT CCACCGACAG CCGCCTCTGG 
GTGGAATTTC GCAGCAGCAG CAATTGGGTC GGAAAGGGCT TCTTTGCAGT CTATGAAGCT 
ATCTGTGGTG GTGATGTGAA AAAAGATAAC GGCCACATCC AGTCACCCAA TTACCCAGAT 
GACTACCGGC CCAGCAAAGT CTGCATCTGG CGGATCCAGG TGTCCGAAGG CTTCCACGTG 
GGCCTCACGT TCCAGTCCTT TGAGATTGAG CGTCATGACA GCTGTGCCTA CGACTACTTG 
GAAGTTCGTG ATGGGCACAG TGAGAGCAGC AACCTCATTG GGCGCTACTG CGGCTATGAG 
AAGCCCGATG ACATCAAAAG CACATCTAGC CGTCTCTGGC TCAAGTTCGT CTCCGATGGA 
TCCATTAACA AAGCTGGCTT CGCAGTCAAT TTTTTCAAAG AGGTGGATGA ATGCTCACGG 
CCCAACCGTG GAGGCTGTGA GCAGCGGTGC CTGAACACCC TGGGCAGCTA CAAGTGCAGC 
TGTGACCCGG GGTACGAGCT GGCCCCCGAC AAGCGCCGAT GTGAGGCTGC CTGTGGTGGA 
TTCCTCACCA AGCTCAACGG CTCCATCACC AGCCCAGGCT GGCCTAAGGA ATACCCTCCC 
AACAAAAACT GCATCTGGCA GTTGGTGGCC CCTACCCAGT ACCGTATCTC CTTGCAGTTT 
GACTTCTTTG AGACGGAGGG CAATGACGTA TGCAAGTATG ACTTTGTGGA GGTACGCAGT 
GGACTCACGG CTGACTCTAA GCTGCATGGC AAGTTCTGTG GCTCTGAGAA ACCAGAAGTC 
ATCACTTCCC AATACAACAA CATGCGTGTG GAATTCAAGT CCGACAATAC TGTATCCAAA 
AAGGGCTTCA AGGCCCACTT CTTCTCAGAC AAGGATGAGT GTTCCAAGGA CAATGGTGGC 
TGCCAGCAAG ACTGCGTGAA CACGTTTGGC AGCTATGAGT GTCAGTGTCG CAGCGGCTTC 
GTCCTCCATG ACAACAAACA TGACTGCAAG GAAGCCGGCT GTGACCACAA GGTGACATCT 
ACCAGTGGCA CCATCACCAG CCCCAACTGG CCTGACAAAT ACCCCAGCAA GAAGGAATGC 
ACGTGGGCTA TCTCCAGCAC CCCTGGGCAT CGGGTCAAGC TGACCTTCAT GGAGATGGAC 
ATTGAGTCTC AGCCCGAGTG TGCTTATGAC CACCTTGAGG TGTTTGATGG GCGTGACACC 
AAGGCACCAG TCCTCGGCCG TTTCTGTGGC AGCAAGAAGC CAGAACCTGT CCTGGCTACA 
GGCAGTCGCA TGTTCCTGCG CTTCTACTCG GACAACTCTG TACAGAGGAA AGGCTTCCAG 
GCATCTCATT CCACAGAGTG TGGAGGCCAA GTTCGAGCAG ATGTGAAGAC CAAAGACCTT 
TATTCCCATG CCCAGTTTGG TGACAACAAC TACCCTGGAG GGGTGGACTG CGAGTGGGTC 
ATTGTGGCTG AGGAGGGCTA TGGTGTAGAA CTGGTGTTCC AGACCTTCGA GGTGGAGGAA 
GAGACCGACT GTGGCTATGA CTACATGGAG CTCTTTGATG GCTACGACAG CACAGCCCCA 
AGACTGGGGC GCTACTGTGG CTCTGGGCCT CCTGAGGAAG TGTACTCGGC TGGAGATTCT 
GTCCTGGTGA AGTTCCATTC TGATGACACC ATCACCAAGA AAGGCTTCCA CCTGCGGTAC 
ACAAGCACCA AGTTTCAGGA CACACTCCAC AGCAGGAAGT GA