Mcf2l gene cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

mRNA	Protein	Name
XM_008848407.1	XP_008846629.1	guanine nucleotide exchange factor DBS isoform X1
XM_008848416.1	XP_008846638.1	guanine nucleotide exchange factor DBS isoform X8
XM_008848417.1	XP_008846639.1	guanine nucleotide exchange factor DBS isoform X9
XM_029553701.1	XP_029409561.1	guanine nucleotide exchange factor DBS isoform X12
XM_029553703.1	XP_029409563.1	guanine nucleotide exchange factor DBS isoform X12
XM_008848408.1	XP_008846630.1	guanine nucleotide exchange factor DBS isoform X2
XM_008848409.2	XP_008846631.1	guanine nucleotide exchange factor DBS isoform X3
XM_008848411.2	XP_008846633.1	guanine nucleotide exchange factor DBS isoform X6
XM_008848414.1	XP_008846636.1	guanine nucleotide exchange factor DBS isoform X7
XM_008848410.2	XP_008846632.1	guanine nucleotide exchange factor DBS isoform X4
XM_008848416.2	XP_008846638.1	guanine nucleotide exchange factor DBS isoform X8
XM_008848417.2	XP_008846639.1	guanine nucleotide exchange factor DBS isoform X9
XM_008848418.2	XP_008846640.1	guanine nucleotide exchange factor DBS isoform X10
XM_029553705.1	XP_029409565.1	guanine nucleotide exchange factor DBS isoform X13
XM_029553706.1	XP_029409566.1	guanine nucleotide exchange factor DBS isoform X14
XM_008848409.3	XP_008846631.1	guanine nucleotide exchange factor DBS isoform X3
XM_008848407.2	XP_008846629.1	guanine nucleotide exchange factor DBS isoform X1
XM_029553699.1	XP_029409559.1	guanine nucleotide exchange factor DBS isoform X1
XM_008848408.2	XP_008846630.1	guanine nucleotide exchange factor DBS isoform X2
XM_008848413.2	XP_008846635.1	guanine nucleotide exchange factor DBS isoform X5
XM_008848411.3	XP_008846633.1	guanine nucleotide exchange factor DBS isoform X6
XM_008848414.2	XP_008846636.1	guanine nucleotide exchange factor DBS isoform X7
XM_029553700.1	XP_029409560.1	guanine nucleotide exchange factor DBS isoform X11
XM_029553702.1	XP_029409562.1	guanine nucleotide exchange factor DBS isoform X12
XM_029553704.1	XP_029409564.1	guanine nucleotide exchange factor DBS isoform X12
XM_008848413.1	XP_008846635.1	guanine nucleotide exchange factor DBS isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONa135171	XM_029553700.1 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ONa135172	XM_029553704.1 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ONa135172	XM_029553702.1 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ONa135172	XM_029553701.1 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ONa135172	XM_029553703.1 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ONa135173	XM_029553705.1 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ONa135174	XM_029553706.1 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ONa31072	XM_008848407.2 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa31072	XM_029553699.1 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa31073	XM_008848408.2 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa31074	XM_008848409.3 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa31075	XM_008848410.2 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa31076	XM_008848413.2 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa31077	XM_008848411.3 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa31078	XM_008848414.2 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa31079	XM_008848416.2 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ONa31080	XM_008848417.2 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ONa31081	XM_008848418.2 Latest version!	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
ONa31079	XM_008848416.1	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ONa31080	XM_008848417.1	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ONa31074	XM_008848409.2	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa31072	XM_008848407.1	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa31073	XM_008848408.1	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa31077	XM_008848411.2	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa31078	XM_008848414.1	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa31076	XM_008848413.1	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

Mcf2l ( XM_029553700.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029409560.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X12, mRNA.

Price & Availability↑

CloneID	ONa135171
Clone ID Related Accession (Same CDS sequence)	XM_029553700.1
Accession Version	XM_029553700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGAAA GGAGGTGGCT TGGCTCTTGT AGTGCAGTAA GTTTGCAAGA TGCTGGCATT 
GGATTCATTC TGGTCATAGA CCGAAGGCAG GACAAATGGA CCTCCGTGAA GGCGTCGGTC 
CTGCGAATAG CCGCATCTTT CCCAGCAAAC CTGCAGCTCG TCCTTGTGCT TCGCCCAACG 
GGCTTTTTCC AGAGGACTCT CTCTGACCTC GCCTTCAAGT TCAACAGAGA TGACTTTAAG 
ATGAAGGTGC CGGTCATGAT GCTGAGCTCA GTGCCAGAAC TACACGGCTA CATTGATAAG 
TCACAGCTGA CCGAGGACCT CGGGGGGACC CTGGACTACT GCCATTCCAG GTGGCTGTGC 
CACCGCACGG CGATCGAAAG CTTCGCCCTG ATGGTGAAGC AGACAGCTCA GATGCTGCAG 
GCCTTCGGGA CCGAGCTGGC CGAAACAGAA CTGCCCAACG ATGTCCAATC AACAAGCTCG 
GTGCTGAGCA CACACACGGA GAAGAAGGAC AAAGCAAAGG AGGATCTGCA GCTGGCACGG 
AAAGAGGGGA ACAGCATCCT TGAGAGCCTC AGGGAGCCAC TGGCTGAAAG CACTGCCCAC 
AGTGTAAACC AAGACCAGCT GGACAATCAG GCCACAGTGC AGAGGCTCCT GGCTCAGCTG 
AATGAAACGG AGGCCGCCTT TGACGAGTTC TGGGCAAAGC ATCAGCAGAA GCTGGAACAG 
TGCCTGCAGC TGCGGCATTT TGAGCAGGGT TTCCGGGAGG TCAAAACTAC CTTGGATACC 
ATGTCCCACA AGATAGCCAC CTTCACTGAC GTTGGTAACA GCCTGGCACA TGTGCAACAC 
CTGCTGAAGG ACCTGGCCAG CTTCGAAGAG AAGTCTAGCG TGGCTGTAGA CAGGGCCCGA 
GCCCTGTCAC TGGAGGGTCA GCAGCTGATT GAGAACAAGC ACTATGCTGT GGACTCCATC 
CACCCTAAGT GTGAGGAGCT CCAGCACCTC TGCGATCACT TCACCGCTGA GATCAGCAGG 
AGGAGAGGCT TGCTCAGCAA GTCCCTGGAG CTACACAGCC TCTTGGAGAC GTCCATGAAG 
TGGTGCGATG AGGGCATCTT CCTGCTGGCC TCGCAACCTG TGGACAAGTG TCAGTCCCAG 
GATGGTGCTG AGGCAGCCCT CCAGGAGATC GAGAAATTTT TGGAGACTGG TGCAGAAAAT 
AAGATTCAAG AACTCAATAA AATTTACAAG GAATATGAGT GCATCCTCAA CCAGGACCTA 
CTGGAACACG TGCAAAAGGT CTTTCAGAAG CAAGAAAGTA TGCAAGAGAT GTTCCACCGC 
AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC AAGCAGACAC GGCCTGTGCA GCCCGTGGCC 
CCACGGCCAG AGGCACTCAC CAAATCACCC TGCCCCTCCC CAGGCTCCTG GAGAGGATCT 
GAGAACTCTA GCTCTGAGGG CAGTGCACTC CGTAGAGGGC CCTACAGGAG AGCCAAGAGT 
GAAATGAGTG AGACCCGGCA GGGCCGTGCC AGCTCCACAG GGGACGAGGA GGAGAGCCTA 
GCCATCCTGC GCAGGCATGT GATGAATGAG CTCCTAGACA CGGAACGGGC CTACGTGGAG 
GAGCTGCTCT GTGTACTAGA GGGCTATGCT GCAGAGATGG ACAACCCCCT GATGGCACAC 
CTCATTTCAG CAGGCCTGCA AAACAAGAAG AACATTCTGT TTGGAAACAT GGAGGAGATT 
TATCACTTTC ATAACAGGAT ATTCCTGCGG GAGCTAGAGA GCTGCATAGA CTGCCCGGAA 
CTGGTGGGGA GGTGCTTTCT GGAGAGGATG GAGGAGTTCC AGATCTACGA GAAGTACTGT 
CAGAACAAGC CACGATCTGA GAGCCTGTGG AGACAGTGCT CTGACTGTCC CTTCTTCCAG 
GAGTGCCAGA AGAAGTTGGA CCACAAACTG AGCCTTGATT CCTACCTGCT GAAGCCAGTG 
CAGAGAATAA CTAAGTACCA GTTGCTGCTT AAGGAAATGT TGAAATACAG CAAGAACTGT 
GAGGGGGCAG AGGACCTACA GGAGGCACTG AGCTCCATCC TGGGCATCCT CAAGGCAGTG 
AATGACTCCA TGCACCTCAT CGCCATCACT GGCTACGACG GTAACCTGGG TGATCTGGGG 
AAGCTGCTGA TGCAAGGTTC CTTCAGTGTA TGGACAGACC ACAAGAAGGG CCACACCAAG 
GTCAAGGAGC TGGCCAGATT CAAGCCCATG CAGCGGCACC TTTTCCTGCA TGAGAAGGCT 
GTGCTGTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAGGC CCCTTCCTAC 
AGCTACAAGC AGTCATTGAA TATGACTGCT GTTGGCATCA CAGAGAATGT GAAGGGTGAT 
GCCAAGAAGT TCGAGATCTG GTACAACGCA AGAGAGGAGG TTTACATCAT CCAGGCACCA 
ACCCCTGAGA TTAAAGCAGC ATGGGTGAGT GAGATTCGGA AGGTGCTGAC CAGCCAGCTG 
CAGGCCTGCC GGGAAGCCAG CCAGCACAGG GCCCTGGAGC AGTCCCACAG CCTGCCTTTG 
CCCACACCAT CCAACACCAG CCCCTCAAAA GGGAACACAA GGAATGTCAA GAAGTCAGAA 
GAAAGAAAAA CGGACCCCCT GAGCCTGGAA GGATATGTGA GCTCATCGTT GCCGAAGCCC 
CCTGAGAAAG GCAAAGACGA TGCGGTCCCT AGTTCTACCT CTGAAAGCTC TGCGCTTTCC 
AGAAAGCGCT TCACGCTGCA GGGCTTGGCT AACCTCAAAG GTCAGAAAGC CTCTCCGACC 
AGTCCTGACA AAAAAGCTAA GCGGCACGAA GTAAAGAGCG ACCCGACTCC CTTTGGTTTG 
CGAGGCTGGA GCAAAACATC CCACTCCCTG GAAGCCCCTG AGGAGGACGG AGGTTGGTCC 
AGTGCTGAGG AGCTTGTCAA TTCATCTGAT GCAGAGGAAG ATGGAGTGGG CCCCAAGAAG 
CTGGTTCCTG GTAAATACAC AGTGGTGATG GACGATGAGA AAGGGGGCCT TGACACCCTT 
GCTGTGAGGA GCGGGGACAT GGTAGAGGTG GTGGAAGAGG GCGCAGAGGG GCTCTGGTAT 
GTCCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT TTCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC 
GTGCTGAGCA ACTCCTCCAG CTGCAGCGAG GGCTGCCCCA CTCCCTTCTC CGACCTCCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029409560.1
CDS	16..3321
Translation	MHERRWLGSCSAVSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_029553700.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029553700.1

ORF Insert Sequence:

ATGCACGAAA GGAGGTGGCT TGGCTCTTGT AGTGCAGTAA GTTTGCAAGA TGCTGGCATT 
GGATTCATTC TGGTCATAGA CCGAAGGCAG GACAAATGGA CCTCCGTGAA GGCGTCGGTC 
CTGCGAATAG CCGCATCTTT CCCAGCAAAC CTGCAGCTCG TCCTTGTGCT TCGCCCAACG 
GGCTTTTTCC AGAGGACTCT CTCTGACCTC GCCTTCAAGT TCAACAGAGA TGACTTTAAG 
ATGAAGGTGC CGGTCATGAT GCTGAGCTCA GTGCCAGAAC TACACGGCTA CATTGATAAG 
TCACAGCTGA CCGAGGACCT CGGGGGGACC CTGGACTACT GCCATTCCAG GTGGCTGTGC 
CACCGCACGG CGATCGAAAG CTTCGCCCTG ATGGTGAAGC AGACAGCTCA GATGCTGCAG 
GCCTTCGGGA CCGAGCTGGC CGAAACAGAA CTGCCCAACG ATGTCCAATC AACAAGCTCG 
GTGCTGAGCA CACACACGGA GAAGAAGGAC AAAGCAAAGG AGGATCTGCA GCTGGCACGG 
AAAGAGGGGA ACAGCATCCT TGAGAGCCTC AGGGAGCCAC TGGCTGAAAG CACTGCCCAC 
AGTGTAAACC AAGACCAGCT GGACAATCAG GCCACAGTGC AGAGGCTCCT GGCTCAGCTG 
AATGAAACGG AGGCCGCCTT TGACGAGTTC TGGGCAAAGC ATCAGCAGAA GCTGGAACAG 
TGCCTGCAGC TGCGGCATTT TGAGCAGGGT TTCCGGGAGG TCAAAACTAC CTTGGATACC 
ATGTCCCACA AGATAGCCAC CTTCACTGAC GTTGGTAACA GCCTGGCACA TGTGCAACAC 
CTGCTGAAGG ACCTGGCCAG CTTCGAAGAG AAGTCTAGCG TGGCTGTAGA CAGGGCCCGA 
GCCCTGTCAC TGGAGGGTCA GCAGCTGATT GAGAACAAGC ACTATGCTGT GGACTCCATC 
CACCCTAAGT GTGAGGAGCT CCAGCACCTC TGCGATCACT TCACCGCTGA GATCAGCAGG 
AGGAGAGGCT TGCTCAGCAA GTCCCTGGAG CTACACAGCC TCTTGGAGAC GTCCATGAAG 
TGGTGCGATG AGGGCATCTT CCTGCTGGCC TCGCAACCTG TGGACAAGTG TCAGTCCCAG 
GATGGTGCTG AGGCAGCCCT CCAGGAGATC GAGAAATTTT TGGAGACTGG TGCAGAAAAT 
AAGATTCAAG AACTCAATAA AATTTACAAG GAATATGAGT GCATCCTCAA CCAGGACCTA 
CTGGAACACG TGCAAAAGGT CTTTCAGAAG CAAGAAAGTA TGCAAGAGAT GTTCCACCGC 
AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC AAGCAGACAC GGCCTGTGCA GCCCGTGGCC 
CCACGGCCAG AGGCACTCAC CAAATCACCC TGCCCCTCCC CAGGCTCCTG GAGAGGATCT 
GAGAACTCTA GCTCTGAGGG CAGTGCACTC CGTAGAGGGC CCTACAGGAG AGCCAAGAGT 
GAAATGAGTG AGACCCGGCA GGGCCGTGCC AGCTCCACAG GGGACGAGGA GGAGAGCCTA 
GCCATCCTGC GCAGGCATGT GATGAATGAG CTCCTAGACA CGGAACGGGC CTACGTGGAG 
GAGCTGCTCT GTGTACTAGA GGGCTATGCT GCAGAGATGG ACAACCCCCT GATGGCACAC 
CTCATTTCAG CAGGCCTGCA AAACAAGAAG AACATTCTGT TTGGAAACAT GGAGGAGATT 
TATCACTTTC ATAACAGGAT ATTCCTGCGG GAGCTAGAGA GCTGCATAGA CTGCCCGGAA 
CTGGTGGGGA GGTGCTTTCT GGAGAGGATG GAGGAGTTCC AGATCTACGA GAAGTACTGT 
CAGAACAAGC CACGATCTGA GAGCCTGTGG AGACAGTGCT CTGACTGTCC CTTCTTCCAG 
GAGTGCCAGA AGAAGTTGGA CCACAAACTG AGCCTTGATT CCTACCTGCT GAAGCCAGTG 
CAGAGAATAA CTAAGTACCA GTTGCTGCTT AAGGAAATGT TGAAATACAG CAAGAACTGT 
GAGGGGGCAG AGGACCTACA GGAGGCACTG AGCTCCATCC TGGGCATCCT CAAGGCAGTG 
AATGACTCCA TGCACCTCAT CGCCATCACT GGCTACGACG GTAACCTGGG TGATCTGGGG 
AAGCTGCTGA TGCAAGGTTC CTTCAGTGTA TGGACAGACC ACAAGAAGGG CCACACCAAG 
GTCAAGGAGC TGGCCAGATT CAAGCCCATG CAGCGGCACC TTTTCCTGCA TGAGAAGGCT 
GTGCTGTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAGGC CCCTTCCTAC 
AGCTACAAGC AGTCATTGAA TATGACTGCT GTTGGCATCA CAGAGAATGT GAAGGGTGAT 
GCCAAGAAGT TCGAGATCTG GTACAACGCA AGAGAGGAGG TTTACATCAT CCAGGCACCA 
ACCCCTGAGA TTAAAGCAGC ATGGGTGAGT GAGATTCGGA AGGTGCTGAC CAGCCAGCTG 
CAGGCCTGCC GGGAAGCCAG CCAGCACAGG GCCCTGGAGC AGTCCCACAG CCTGCCTTTG 
CCCACACCAT CCAACACCAG CCCCTCAAAA GGGAACACAA GGAATGTCAA GAAGTCAGAA 
GAAAGAAAAA CGGACCCCCT GAGCCTGGAA GGATATGTGA GCTCATCGTT GCCGAAGCCC 
CCTGAGAAAG GCAAAGACGA TGCGGTCCCT AGTTCTACCT CTGAAAGCTC TGCGCTTTCC 
AGAAAGCGCT TCACGCTGCA GGGCTTGGCT AACCTCAAAG GTCAGAAAGC CTCTCCGACC 
AGTCCTGACA AAAAAGCTAA GCGGCACGAA GTAAAGAGCG ACCCGACTCC CTTTGGTTTG 
CGAGGCTGGA GCAAAACATC CCACTCCCTG GAAGCCCCTG AGGAGGACGG AGGTTGGTCC 
AGTGCTGAGG AGCTTGTCAA TTCATCTGAT GCAGAGGAAG ATGGAGTGGG CCCCAAGAAG 
CTGGTTCCTG GTAAATACAC AGTGGTGATG GACGATGAGA AAGGGGGCCT TGACACCCTT 
GCTGTGAGGA GCGGGGACAT GGTAGAGGTG GTGGAAGAGG GCGCAGAGGG GCTCTGGTAT 
GTCCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT TTCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC 
GTGCTGAGCA ACTCCTCCAG CTGCAGCGAG GGCTGCCCCA CTCCCTTCTC CGACCTCCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_029553701.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029409561.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X13, mRNA.

Price & Availability↑

CloneID	ONa135172
Clone ID Related Accession (Same CDS sequence)	XM_029553701.1 , XM_029553703.1 , XM_029553702.1 , XM_029553704.1
Accession Version	XM_029553701.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTGC CGGTCATGAT GCTGAGCTCA GTGCCAGAAC TACACGGCTA CATTGATAAG 
TCACAGCTGA CCGAGGACCT CGGGGGGACC CTGGACTACT GCCATTCCAG GTGGCTGTGC 
CACCGCACGG CGATCGAAAG CTTCGCCCTG ATGGTGAAGC AGACAGCTCA GATGCTGCAG 
GCCTTCGGGA CCGAGCTGGC CGAAACAGAA CTGCCCAACG ATGTCCAATC AACAAGCTCG 
GTGCTGAGCA CACACACGGA GAAGAAGGAC AAAGCAAAGG AGGATCTGCA GCTGGCACGG 
AAAGAGGGGA ACAGCATCCT TGAGAGCCTC AGGGAGCCAC TGGCTGAAAG CACTGCCCAC 
AGTGTAAACC AAGACCAGCT GGACAATCAG GCCACAGTGC AGAGGCTCCT GGCTCAGCTG 
AATGAAACGG AGGCCGCCTT TGACGAGTTC TGGGCAAAGC ATCAGCAGAA GCTGGAACAG 
TGCCTGCAGC TGCGGCATTT TGAGCAGGGT TTCCGGGAGG TCAAAACTAC CTTGGATACC 
ATGTCCCACA AGATAGCCAC CTTCACTGAC GTTGGTAACA GCCTGGCACA TGTGCAACAC 
CTGCTGAAGG ACCTGGCCAG CTTCGAAGAG AAGTCTAGCG TGGCTGTAGA CAGGGCCCGA 
GCCCTGTCAC TGGAGGGTCA GCAGCTGATT GAGAACAAGC ACTATGCTGT GGACTCCATC 
CACCCTAAGT GTGAGGAGCT CCAGCACCTC TGCGATCACT TCACCGCTGA GATCAGCAGG 
AGGAGAGGCT TGCTCAGCAA GTCCCTGGAG CTACACAGCC TCTTGGAGAC GTCCATGAAG 
TGGTGCGATG AGGGCATCTT CCTGCTGGCC TCGCAACCTG TGGACAAGTG TCAGTCCCAG 
GATGGTGCTG AGGCAGCCCT CCAGGAGATC GAGAAATTTT TGGAGACTGG TGCAGAAAAT 
AAGATTCAAG AACTCAATAA AATTTACAAG GAATATGAGT GCATCCTCAA CCAGGACCTA 
CTGGAACACG TGCAAAAGGT CTTTCAGAAG CAAGAAAGTA TGCAAGAGAT GTTCCACCGC 
AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC AAGCAGACAC GGCCTGTGCA GCCCGTGGCC 
CCACGGCCAG AGGCACTCAC CAAATCACCC TGCCCCTCCC CAGGCTCCTG GAGAGGATCT 
GAGAACTCTA GCTCTGAGGG CAGTGCACTC CGTAGAGGGC CCTACAGGAG AGCCAAGAGT 
GAAATGAGTG AGACCCGGCA GGGCCGTGCC AGCTCCACAG GGGACGAGGA GGAGAGCCTA 
GCCATCCTGC GCAGGCATGT GATGAATGAG CTCCTAGACA CGGAACGGGC CTACGTGGAG 
GAGCTGCTCT GTGTACTAGA GGGCTATGCT GCAGAGATGG ACAACCCCCT GATGGCACAC 
CTCATTTCAG CAGGCCTGCA AAACAAGAAG AACATTCTGT TTGGAAACAT GGAGGAGATT 
TATCACTTTC ATAACAGGAT ATTCCTGCGG GAGCTAGAGA GCTGCATAGA CTGCCCGGAA 
CTGGTGGGGA GGTGCTTTCT GGAGAGGATG GAGGAGTTCC AGATCTACGA GAAGTACTGT 
CAGAACAAGC CACGATCTGA GAGCCTGTGG AGACAGTGCT CTGACTGTCC CTTCTTCCAG 
GAGTGCCAGA AGAAGTTGGA CCACAAACTG AGCCTTGATT CCTACCTGCT GAAGCCAGTG 
CAGAGAATAA CTAAGTACCA GTTGCTGCTT AAGGAAATGT TGAAATACAG CAAGAACTGT 
GAGGGGGCAG AGGACCTACA GGAGGCACTG AGCTCCATCC TGGGCATCCT CAAGGCAGTG 
AATGACTCCA TGCACCTCAT CGCCATCACT GGCTACGACG GTAACCTGGG TGATCTGGGG 
AAGCTGCTGA TGCAAGGTTC CTTCAGTGTA TGGACAGACC ACAAGAAGGG CCACACCAAG 
GTCAAGGAGC TGGCCAGATT CAAGCCCATG CAGCGGCACC TTTTCCTGCA TGAGAAGGCT 
GTGCTGTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAGGC CCCTTCCTAC 
AGCTACAAGC AGTCATTGAA TATGACTGCT GTTGGCATCA CAGAGAATGT GAAGGGTGAT 
GCCAAGAAGT TCGAGATCTG GTACAACGCA AGAGAGGAGG TTTACATCAT CCAGGCACCA 
ACCCCTGAGA TTAAAGCAGC ATGGGTGAGT GAGATTCGGA AGGTGCTGAC CAGCCAGCTG 
CAGGCCTGCC GGGAAGCCAG CCAGCACAGG GCCCTGGAGC AGTCCCACAG CCTGCCTTTG 
CCCACACCAT CCAACACCAG CCCCTCAAAA GGGAACACAA GGAATGTCAA GAAGTCAGAA 
GAAAGAAAAA CGGACCCCCT GAGCCTGGAA GGATATGTGA GCTCATCGTT GCCGAAGCCC 
CCTGAGAAAG GCAAAGACGA TGCGGTCCCT AGTTCTACCT CTGAAAGCTC TGCGCTTTCC 
AGAAAGCGCT TCACGCTGCA GGGCTTGGCT AACCTCAAAG GTCAGAAAGC CTCTCCGACC 
AGTCCTGACA AAAAAGCTAA GCGGCACGAA GTAAAGAGCG ACCCGACTCC CTTTGGTTTG 
CGAGGCTGGA GCAAAACATC CCACTCCCTG GAAGCCCCTG AGGAGGACGG AGGTTGGTCC 
AGTGCTGAGG AGCTTGTCAA TTCATCTGAT GCAGAGGAAG ATGGAGTGGG CCCCAAGAAG 
CTGGTTCCTG GTAAATACAC AGTGGTGATG GACGATGAGA AAGGGGGCCT TGACACCCTT 
GCTGTGAGGA GCGGGGACAT GGTAGAGGTG GTGGAAGAGG GCGCAGAGGG GCTCTGGTAT 
GTCCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT TTCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC 
GTGCTGAGCA ACTCCTCCAG CTGCAGCGAG GGCTGCCCCA CTCCCTTCTC CGACCTCCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029409561.1
CDS	379..3444
Translation	MKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_029553701.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029553701.1

ORF Insert Sequence:

ATGAAGGTGC CGGTCATGAT GCTGAGCTCA GTGCCAGAAC TACACGGCTA CATTGATAAG 
TCACAGCTGA CCGAGGACCT CGGGGGGACC CTGGACTACT GCCATTCCAG GTGGCTGTGC 
CACCGCACGG CGATCGAAAG CTTCGCCCTG ATGGTGAAGC AGACAGCTCA GATGCTGCAG 
GCCTTCGGGA CCGAGCTGGC CGAAACAGAA CTGCCCAACG ATGTCCAATC AACAAGCTCG 
GTGCTGAGCA CACACACGGA GAAGAAGGAC AAAGCAAAGG AGGATCTGCA GCTGGCACGG 
AAAGAGGGGA ACAGCATCCT TGAGAGCCTC AGGGAGCCAC TGGCTGAAAG CACTGCCCAC 
AGTGTAAACC AAGACCAGCT GGACAATCAG GCCACAGTGC AGAGGCTCCT GGCTCAGCTG 
AATGAAACGG AGGCCGCCTT TGACGAGTTC TGGGCAAAGC ATCAGCAGAA GCTGGAACAG 
TGCCTGCAGC TGCGGCATTT TGAGCAGGGT TTCCGGGAGG TCAAAACTAC CTTGGATACC 
ATGTCCCACA AGATAGCCAC CTTCACTGAC GTTGGTAACA GCCTGGCACA TGTGCAACAC 
CTGCTGAAGG ACCTGGCCAG CTTCGAAGAG AAGTCTAGCG TGGCTGTAGA CAGGGCCCGA 
GCCCTGTCAC TGGAGGGTCA GCAGCTGATT GAGAACAAGC ACTATGCTGT GGACTCCATC 
CACCCTAAGT GTGAGGAGCT CCAGCACCTC TGCGATCACT TCACCGCTGA GATCAGCAGG 
AGGAGAGGCT TGCTCAGCAA GTCCCTGGAG CTACACAGCC TCTTGGAGAC GTCCATGAAG 
TGGTGCGATG AGGGCATCTT CCTGCTGGCC TCGCAACCTG TGGACAAGTG TCAGTCCCAG 
GATGGTGCTG AGGCAGCCCT CCAGGAGATC GAGAAATTTT TGGAGACTGG TGCAGAAAAT 
AAGATTCAAG AACTCAATAA AATTTACAAG GAATATGAGT GCATCCTCAA CCAGGACCTA 
CTGGAACACG TGCAAAAGGT CTTTCAGAAG CAAGAAAGTA TGCAAGAGAT GTTCCACCGC 
AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC AAGCAGACAC GGCCTGTGCA GCCCGTGGCC 
CCACGGCCAG AGGCACTCAC CAAATCACCC TGCCCCTCCC CAGGCTCCTG GAGAGGATCT 
GAGAACTCTA GCTCTGAGGG CAGTGCACTC CGTAGAGGGC CCTACAGGAG AGCCAAGAGT 
GAAATGAGTG AGACCCGGCA GGGCCGTGCC AGCTCCACAG GGGACGAGGA GGAGAGCCTA 
GCCATCCTGC GCAGGCATGT GATGAATGAG CTCCTAGACA CGGAACGGGC CTACGTGGAG 
GAGCTGCTCT GTGTACTAGA GGGCTATGCT GCAGAGATGG ACAACCCCCT GATGGCACAC 
CTCATTTCAG CAGGCCTGCA AAACAAGAAG AACATTCTGT TTGGAAACAT GGAGGAGATT 
TATCACTTTC ATAACAGGAT ATTCCTGCGG GAGCTAGAGA GCTGCATAGA CTGCCCGGAA 
CTGGTGGGGA GGTGCTTTCT GGAGAGGATG GAGGAGTTCC AGATCTACGA GAAGTACTGT 
CAGAACAAGC CACGATCTGA GAGCCTGTGG AGACAGTGCT CTGACTGTCC CTTCTTCCAG 
GAGTGCCAGA AGAAGTTGGA CCACAAACTG AGCCTTGATT CCTACCTGCT GAAGCCAGTG 
CAGAGAATAA CTAAGTACCA GTTGCTGCTT AAGGAAATGT TGAAATACAG CAAGAACTGT 
GAGGGGGCAG AGGACCTACA GGAGGCACTG AGCTCCATCC TGGGCATCCT CAAGGCAGTG 
AATGACTCCA TGCACCTCAT CGCCATCACT GGCTACGACG GTAACCTGGG TGATCTGGGG 
AAGCTGCTGA TGCAAGGTTC CTTCAGTGTA TGGACAGACC ACAAGAAGGG CCACACCAAG 
GTCAAGGAGC TGGCCAGATT CAAGCCCATG CAGCGGCACC TTTTCCTGCA TGAGAAGGCT 
GTGCTGTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAGGC CCCTTCCTAC 
AGCTACAAGC AGTCATTGAA TATGACTGCT GTTGGCATCA CAGAGAATGT GAAGGGTGAT 
GCCAAGAAGT TCGAGATCTG GTACAACGCA AGAGAGGAGG TTTACATCAT CCAGGCACCA 
ACCCCTGAGA TTAAAGCAGC ATGGGTGAGT GAGATTCGGA AGGTGCTGAC CAGCCAGCTG 
CAGGCCTGCC GGGAAGCCAG CCAGCACAGG GCCCTGGAGC AGTCCCACAG CCTGCCTTTG 
CCCACACCAT CCAACACCAG CCCCTCAAAA GGGAACACAA GGAATGTCAA GAAGTCAGAA 
GAAAGAAAAA CGGACCCCCT GAGCCTGGAA GGATATGTGA GCTCATCGTT GCCGAAGCCC 
CCTGAGAAAG GCAAAGACGA TGCGGTCCCT AGTTCTACCT CTGAAAGCTC TGCGCTTTCC 
AGAAAGCGCT TCACGCTGCA GGGCTTGGCT AACCTCAAAG GTCAGAAAGC CTCTCCGACC 
AGTCCTGACA AAAAAGCTAA GCGGCACGAA GTAAAGAGCG ACCCGACTCC CTTTGGTTTG 
CGAGGCTGGA GCAAAACATC CCACTCCCTG GAAGCCCCTG AGGAGGACGG AGGTTGGTCC 
AGTGCTGAGG AGCTTGTCAA TTCATCTGAT GCAGAGGAAG ATGGAGTGGG CCCCAAGAAG 
CTGGTTCCTG GTAAATACAC AGTGGTGATG GACGATGAGA AAGGGGGCCT TGACACCCTT 
GCTGTGAGGA GCGGGGACAT GGTAGAGGTG GTGGAAGAGG GCGCAGAGGG GCTCTGGTAT 
GTCCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT TTCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC 
GTGCTGAGCA ACTCCTCCAG CTGCAGCGAG GGCTGCCCCA CTCCCTTCTC CGACCTCCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_029553703.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029409563.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X15, mRNA.

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CloneID	ONa135172
Clone ID Related Accession (Same CDS sequence)	XM_029553701.1 , XM_029553703.1 , XM_029553702.1 , XM_029553704.1
Accession Version	XM_029553703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTGC CGGTCATGAT GCTGAGCTCA GTGCCAGAAC TACACGGCTA CATTGATAAG 
TCACAGCTGA CCGAGGACCT CGGGGGGACC CTGGACTACT GCCATTCCAG GTGGCTGTGC 
CACCGCACGG CGATCGAAAG CTTCGCCCTG ATGGTGAAGC AGACAGCTCA GATGCTGCAG 
GCCTTCGGGA CCGAGCTGGC CGAAACAGAA CTGCCCAACG ATGTCCAATC AACAAGCTCG 
GTGCTGAGCA CACACACGGA GAAGAAGGAC AAAGCAAAGG AGGATCTGCA GCTGGCACGG 
AAAGAGGGGA ACAGCATCCT TGAGAGCCTC AGGGAGCCAC TGGCTGAAAG CACTGCCCAC 
AGTGTAAACC AAGACCAGCT GGACAATCAG GCCACAGTGC AGAGGCTCCT GGCTCAGCTG 
AATGAAACGG AGGCCGCCTT TGACGAGTTC TGGGCAAAGC ATCAGCAGAA GCTGGAACAG 
TGCCTGCAGC TGCGGCATTT TGAGCAGGGT TTCCGGGAGG TCAAAACTAC CTTGGATACC 
ATGTCCCACA AGATAGCCAC CTTCACTGAC GTTGGTAACA GCCTGGCACA TGTGCAACAC 
CTGCTGAAGG ACCTGGCCAG CTTCGAAGAG AAGTCTAGCG TGGCTGTAGA CAGGGCCCGA 
GCCCTGTCAC TGGAGGGTCA GCAGCTGATT GAGAACAAGC ACTATGCTGT GGACTCCATC 
CACCCTAAGT GTGAGGAGCT CCAGCACCTC TGCGATCACT TCACCGCTGA GATCAGCAGG 
AGGAGAGGCT TGCTCAGCAA GTCCCTGGAG CTACACAGCC TCTTGGAGAC GTCCATGAAG 
TGGTGCGATG AGGGCATCTT CCTGCTGGCC TCGCAACCTG TGGACAAGTG TCAGTCCCAG 
GATGGTGCTG AGGCAGCCCT CCAGGAGATC GAGAAATTTT TGGAGACTGG TGCAGAAAAT 
AAGATTCAAG AACTCAATAA AATTTACAAG GAATATGAGT GCATCCTCAA CCAGGACCTA 
CTGGAACACG TGCAAAAGGT CTTTCAGAAG CAAGAAAGTA TGCAAGAGAT GTTCCACCGC 
AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC AAGCAGACAC GGCCTGTGCA GCCCGTGGCC 
CCACGGCCAG AGGCACTCAC CAAATCACCC TGCCCCTCCC CAGGCTCCTG GAGAGGATCT 
GAGAACTCTA GCTCTGAGGG CAGTGCACTC CGTAGAGGGC CCTACAGGAG AGCCAAGAGT 
GAAATGAGTG AGACCCGGCA GGGCCGTGCC AGCTCCACAG GGGACGAGGA GGAGAGCCTA 
GCCATCCTGC GCAGGCATGT GATGAATGAG CTCCTAGACA CGGAACGGGC CTACGTGGAG 
GAGCTGCTCT GTGTACTAGA GGGCTATGCT GCAGAGATGG ACAACCCCCT GATGGCACAC 
CTCATTTCAG CAGGCCTGCA AAACAAGAAG AACATTCTGT TTGGAAACAT GGAGGAGATT 
TATCACTTTC ATAACAGGAT ATTCCTGCGG GAGCTAGAGA GCTGCATAGA CTGCCCGGAA 
CTGGTGGGGA GGTGCTTTCT GGAGAGGATG GAGGAGTTCC AGATCTACGA GAAGTACTGT 
CAGAACAAGC CACGATCTGA GAGCCTGTGG AGACAGTGCT CTGACTGTCC CTTCTTCCAG 
GAGTGCCAGA AGAAGTTGGA CCACAAACTG AGCCTTGATT CCTACCTGCT GAAGCCAGTG 
CAGAGAATAA CTAAGTACCA GTTGCTGCTT AAGGAAATGT TGAAATACAG CAAGAACTGT 
GAGGGGGCAG AGGACCTACA GGAGGCACTG AGCTCCATCC TGGGCATCCT CAAGGCAGTG 
AATGACTCCA TGCACCTCAT CGCCATCACT GGCTACGACG GTAACCTGGG TGATCTGGGG 
AAGCTGCTGA TGCAAGGTTC CTTCAGTGTA TGGACAGACC ACAAGAAGGG CCACACCAAG 
GTCAAGGAGC TGGCCAGATT CAAGCCCATG CAGCGGCACC TTTTCCTGCA TGAGAAGGCT 
GTGCTGTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAGGC CCCTTCCTAC 
AGCTACAAGC AGTCATTGAA TATGACTGCT GTTGGCATCA CAGAGAATGT GAAGGGTGAT 
GCCAAGAAGT TCGAGATCTG GTACAACGCA AGAGAGGAGG TTTACATCAT CCAGGCACCA 
ACCCCTGAGA TTAAAGCAGC ATGGGTGAGT GAGATTCGGA AGGTGCTGAC CAGCCAGCTG 
CAGGCCTGCC GGGAAGCCAG CCAGCACAGG GCCCTGGAGC AGTCCCACAG CCTGCCTTTG 
CCCACACCAT CCAACACCAG CCCCTCAAAA GGGAACACAA GGAATGTCAA GAAGTCAGAA 
GAAAGAAAAA CGGACCCCCT GAGCCTGGAA GGATATGTGA GCTCATCGTT GCCGAAGCCC 
CCTGAGAAAG GCAAAGACGA TGCGGTCCCT AGTTCTACCT CTGAAAGCTC TGCGCTTTCC 
AGAAAGCGCT TCACGCTGCA GGGCTTGGCT AACCTCAAAG GTCAGAAAGC CTCTCCGACC 
AGTCCTGACA AAAAAGCTAA GCGGCACGAA GTAAAGAGCG ACCCGACTCC CTTTGGTTTG 
CGAGGCTGGA GCAAAACATC CCACTCCCTG GAAGCCCCTG AGGAGGACGG AGGTTGGTCC 
AGTGCTGAGG AGCTTGTCAA TTCATCTGAT GCAGAGGAAG ATGGAGTGGG CCCCAAGAAG 
CTGGTTCCTG GTAAATACAC AGTGGTGATG GACGATGAGA AAGGGGGCCT TGACACCCTT 
GCTGTGAGGA GCGGGGACAT GGTAGAGGTG GTGGAAGAGG GCGCAGAGGG GCTCTGGTAT 
GTCCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT TTCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC 
GTGCTGAGCA ACTCCTCCAG CTGCAGCGAG GGCTGCCCCA CTCCCTTCTC CGACCTCCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029409563.1
CDS	376..3441
Translation	MKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_029553703.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029553703.1

ORF Insert Sequence:

ATGAAGGTGC CGGTCATGAT GCTGAGCTCA GTGCCAGAAC TACACGGCTA CATTGATAAG 
TCACAGCTGA CCGAGGACCT CGGGGGGACC CTGGACTACT GCCATTCCAG GTGGCTGTGC 
CACCGCACGG CGATCGAAAG CTTCGCCCTG ATGGTGAAGC AGACAGCTCA GATGCTGCAG 
GCCTTCGGGA CCGAGCTGGC CGAAACAGAA CTGCCCAACG ATGTCCAATC AACAAGCTCG 
GTGCTGAGCA CACACACGGA GAAGAAGGAC AAAGCAAAGG AGGATCTGCA GCTGGCACGG 
AAAGAGGGGA ACAGCATCCT TGAGAGCCTC AGGGAGCCAC TGGCTGAAAG CACTGCCCAC 
AGTGTAAACC AAGACCAGCT GGACAATCAG GCCACAGTGC AGAGGCTCCT GGCTCAGCTG 
AATGAAACGG AGGCCGCCTT TGACGAGTTC TGGGCAAAGC ATCAGCAGAA GCTGGAACAG 
TGCCTGCAGC TGCGGCATTT TGAGCAGGGT TTCCGGGAGG TCAAAACTAC CTTGGATACC 
ATGTCCCACA AGATAGCCAC CTTCACTGAC GTTGGTAACA GCCTGGCACA TGTGCAACAC 
CTGCTGAAGG ACCTGGCCAG CTTCGAAGAG AAGTCTAGCG TGGCTGTAGA CAGGGCCCGA 
GCCCTGTCAC TGGAGGGTCA GCAGCTGATT GAGAACAAGC ACTATGCTGT GGACTCCATC 
CACCCTAAGT GTGAGGAGCT CCAGCACCTC TGCGATCACT TCACCGCTGA GATCAGCAGG 
AGGAGAGGCT TGCTCAGCAA GTCCCTGGAG CTACACAGCC TCTTGGAGAC GTCCATGAAG 
TGGTGCGATG AGGGCATCTT CCTGCTGGCC TCGCAACCTG TGGACAAGTG TCAGTCCCAG 
GATGGTGCTG AGGCAGCCCT CCAGGAGATC GAGAAATTTT TGGAGACTGG TGCAGAAAAT 
AAGATTCAAG AACTCAATAA AATTTACAAG GAATATGAGT GCATCCTCAA CCAGGACCTA 
CTGGAACACG TGCAAAAGGT CTTTCAGAAG CAAGAAAGTA TGCAAGAGAT GTTCCACCGC 
AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC AAGCAGACAC GGCCTGTGCA GCCCGTGGCC 
CCACGGCCAG AGGCACTCAC CAAATCACCC TGCCCCTCCC CAGGCTCCTG GAGAGGATCT 
GAGAACTCTA GCTCTGAGGG CAGTGCACTC CGTAGAGGGC CCTACAGGAG AGCCAAGAGT 
GAAATGAGTG AGACCCGGCA GGGCCGTGCC AGCTCCACAG GGGACGAGGA GGAGAGCCTA 
GCCATCCTGC GCAGGCATGT GATGAATGAG CTCCTAGACA CGGAACGGGC CTACGTGGAG 
GAGCTGCTCT GTGTACTAGA GGGCTATGCT GCAGAGATGG ACAACCCCCT GATGGCACAC 
CTCATTTCAG CAGGCCTGCA AAACAAGAAG AACATTCTGT TTGGAAACAT GGAGGAGATT 
TATCACTTTC ATAACAGGAT ATTCCTGCGG GAGCTAGAGA GCTGCATAGA CTGCCCGGAA 
CTGGTGGGGA GGTGCTTTCT GGAGAGGATG GAGGAGTTCC AGATCTACGA GAAGTACTGT 
CAGAACAAGC CACGATCTGA GAGCCTGTGG AGACAGTGCT CTGACTGTCC CTTCTTCCAG 
GAGTGCCAGA AGAAGTTGGA CCACAAACTG AGCCTTGATT CCTACCTGCT GAAGCCAGTG 
CAGAGAATAA CTAAGTACCA GTTGCTGCTT AAGGAAATGT TGAAATACAG CAAGAACTGT 
GAGGGGGCAG AGGACCTACA GGAGGCACTG AGCTCCATCC TGGGCATCCT CAAGGCAGTG 
AATGACTCCA TGCACCTCAT CGCCATCACT GGCTACGACG GTAACCTGGG TGATCTGGGG 
AAGCTGCTGA TGCAAGGTTC CTTCAGTGTA TGGACAGACC ACAAGAAGGG CCACACCAAG 
GTCAAGGAGC TGGCCAGATT CAAGCCCATG CAGCGGCACC TTTTCCTGCA TGAGAAGGCT 
GTGCTGTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAGGC CCCTTCCTAC 
AGCTACAAGC AGTCATTGAA TATGACTGCT GTTGGCATCA CAGAGAATGT GAAGGGTGAT 
GCCAAGAAGT TCGAGATCTG GTACAACGCA AGAGAGGAGG TTTACATCAT CCAGGCACCA 
ACCCCTGAGA TTAAAGCAGC ATGGGTGAGT GAGATTCGGA AGGTGCTGAC CAGCCAGCTG 
CAGGCCTGCC GGGAAGCCAG CCAGCACAGG GCCCTGGAGC AGTCCCACAG CCTGCCTTTG 
CCCACACCAT CCAACACCAG CCCCTCAAAA GGGAACACAA GGAATGTCAA GAAGTCAGAA 
GAAAGAAAAA CGGACCCCCT GAGCCTGGAA GGATATGTGA GCTCATCGTT GCCGAAGCCC 
CCTGAGAAAG GCAAAGACGA TGCGGTCCCT AGTTCTACCT CTGAAAGCTC TGCGCTTTCC 
AGAAAGCGCT TCACGCTGCA GGGCTTGGCT AACCTCAAAG GTCAGAAAGC CTCTCCGACC 
AGTCCTGACA AAAAAGCTAA GCGGCACGAA GTAAAGAGCG ACCCGACTCC CTTTGGTTTG 
CGAGGCTGGA GCAAAACATC CCACTCCCTG GAAGCCCCTG AGGAGGACGG AGGTTGGTCC 
AGTGCTGAGG AGCTTGTCAA TTCATCTGAT GCAGAGGAAG ATGGAGTGGG CCCCAAGAAG 
CTGGTTCCTG GTAAATACAC AGTGGTGATG GACGATGAGA AAGGGGGCCT TGACACCCTT 
GCTGTGAGGA GCGGGGACAT GGTAGAGGTG GTGGAAGAGG GCGCAGAGGG GCTCTGGTAT 
GTCCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT TTCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC 
GTGCTGAGCA ACTCCTCCAG CTGCAGCGAG GGCTGCCCCA CTCCCTTCTC CGACCTCCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_029553702.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029409562.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ONa135172
Clone ID Related Accession (Same CDS sequence)	XM_029553701.1 , XM_029553703.1 , XM_029553702.1 , XM_029553704.1
Accession Version	XM_029553702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTGC CGGTCATGAT GCTGAGCTCA GTGCCAGAAC TACACGGCTA CATTGATAAG 
TCACAGCTGA CCGAGGACCT CGGGGGGACC CTGGACTACT GCCATTCCAG GTGGCTGTGC 
CACCGCACGG CGATCGAAAG CTTCGCCCTG ATGGTGAAGC AGACAGCTCA GATGCTGCAG 
GCCTTCGGGA CCGAGCTGGC CGAAACAGAA CTGCCCAACG ATGTCCAATC AACAAGCTCG 
GTGCTGAGCA CACACACGGA GAAGAAGGAC AAAGCAAAGG AGGATCTGCA GCTGGCACGG 
AAAGAGGGGA ACAGCATCCT TGAGAGCCTC AGGGAGCCAC TGGCTGAAAG CACTGCCCAC 
AGTGTAAACC AAGACCAGCT GGACAATCAG GCCACAGTGC AGAGGCTCCT GGCTCAGCTG 
AATGAAACGG AGGCCGCCTT TGACGAGTTC TGGGCAAAGC ATCAGCAGAA GCTGGAACAG 
TGCCTGCAGC TGCGGCATTT TGAGCAGGGT TTCCGGGAGG TCAAAACTAC CTTGGATACC 
ATGTCCCACA AGATAGCCAC CTTCACTGAC GTTGGTAACA GCCTGGCACA TGTGCAACAC 
CTGCTGAAGG ACCTGGCCAG CTTCGAAGAG AAGTCTAGCG TGGCTGTAGA CAGGGCCCGA 
GCCCTGTCAC TGGAGGGTCA GCAGCTGATT GAGAACAAGC ACTATGCTGT GGACTCCATC 
CACCCTAAGT GTGAGGAGCT CCAGCACCTC TGCGATCACT TCACCGCTGA GATCAGCAGG 
AGGAGAGGCT TGCTCAGCAA GTCCCTGGAG CTACACAGCC TCTTGGAGAC GTCCATGAAG 
TGGTGCGATG AGGGCATCTT CCTGCTGGCC TCGCAACCTG TGGACAAGTG TCAGTCCCAG 
GATGGTGCTG AGGCAGCCCT CCAGGAGATC GAGAAATTTT TGGAGACTGG TGCAGAAAAT 
AAGATTCAAG AACTCAATAA AATTTACAAG GAATATGAGT GCATCCTCAA CCAGGACCTA 
CTGGAACACG TGCAAAAGGT CTTTCAGAAG CAAGAAAGTA TGCAAGAGAT GTTCCACCGC 
AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC AAGCAGACAC GGCCTGTGCA GCCCGTGGCC 
CCACGGCCAG AGGCACTCAC CAAATCACCC TGCCCCTCCC CAGGCTCCTG GAGAGGATCT 
GAGAACTCTA GCTCTGAGGG CAGTGCACTC CGTAGAGGGC CCTACAGGAG AGCCAAGAGT 
GAAATGAGTG AGACCCGGCA GGGCCGTGCC AGCTCCACAG GGGACGAGGA GGAGAGCCTA 
GCCATCCTGC GCAGGCATGT GATGAATGAG CTCCTAGACA CGGAACGGGC CTACGTGGAG 
GAGCTGCTCT GTGTACTAGA GGGCTATGCT GCAGAGATGG ACAACCCCCT GATGGCACAC 
CTCATTTCAG CAGGCCTGCA AAACAAGAAG AACATTCTGT TTGGAAACAT GGAGGAGATT 
TATCACTTTC ATAACAGGAT ATTCCTGCGG GAGCTAGAGA GCTGCATAGA CTGCCCGGAA 
CTGGTGGGGA GGTGCTTTCT GGAGAGGATG GAGGAGTTCC AGATCTACGA GAAGTACTGT 
CAGAACAAGC CACGATCTGA GAGCCTGTGG AGACAGTGCT CTGACTGTCC CTTCTTCCAG 
GAGTGCCAGA AGAAGTTGGA CCACAAACTG AGCCTTGATT CCTACCTGCT GAAGCCAGTG 
CAGAGAATAA CTAAGTACCA GTTGCTGCTT AAGGAAATGT TGAAATACAG CAAGAACTGT 
GAGGGGGCAG AGGACCTACA GGAGGCACTG AGCTCCATCC TGGGCATCCT CAAGGCAGTG 
AATGACTCCA TGCACCTCAT CGCCATCACT GGCTACGACG GTAACCTGGG TGATCTGGGG 
AAGCTGCTGA TGCAAGGTTC CTTCAGTGTA TGGACAGACC ACAAGAAGGG CCACACCAAG 
GTCAAGGAGC TGGCCAGATT CAAGCCCATG CAGCGGCACC TTTTCCTGCA TGAGAAGGCT 
GTGCTGTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAGGC CCCTTCCTAC 
AGCTACAAGC AGTCATTGAA TATGACTGCT GTTGGCATCA CAGAGAATGT GAAGGGTGAT 
GCCAAGAAGT TCGAGATCTG GTACAACGCA AGAGAGGAGG TTTACATCAT CCAGGCACCA 
ACCCCTGAGA TTAAAGCAGC ATGGGTGAGT GAGATTCGGA AGGTGCTGAC CAGCCAGCTG 
CAGGCCTGCC GGGAAGCCAG CCAGCACAGG GCCCTGGAGC AGTCCCACAG CCTGCCTTTG 
CCCACACCAT CCAACACCAG CCCCTCAAAA GGGAACACAA GGAATGTCAA GAAGTCAGAA 
GAAAGAAAAA CGGACCCCCT GAGCCTGGAA GGATATGTGA GCTCATCGTT GCCGAAGCCC 
CCTGAGAAAG GCAAAGACGA TGCGGTCCCT AGTTCTACCT CTGAAAGCTC TGCGCTTTCC 
AGAAAGCGCT TCACGCTGCA GGGCTTGGCT AACCTCAAAG GTCAGAAAGC CTCTCCGACC 
AGTCCTGACA AAAAAGCTAA GCGGCACGAA GTAAAGAGCG ACCCGACTCC CTTTGGTTTG 
CGAGGCTGGA GCAAAACATC CCACTCCCTG GAAGCCCCTG AGGAGGACGG AGGTTGGTCC 
AGTGCTGAGG AGCTTGTCAA TTCATCTGAT GCAGAGGAAG ATGGAGTGGG CCCCAAGAAG 
CTGGTTCCTG GTAAATACAC AGTGGTGATG GACGATGAGA AAGGGGGCCT TGACACCCTT 
GCTGTGAGGA GCGGGGACAT GGTAGAGGTG GTGGAAGAGG GCGCAGAGGG GCTCTGGTAT 
GTCCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT TTCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC 
GTGCTGAGCA ACTCCTCCAG CTGCAGCGAG GGCTGCCCCA CTCCCTTCTC CGACCTCCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029409562.1
CDS	413..3478
Translation	MKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_029553702.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029553702.1

ORF Insert Sequence:

ATGAAGGTGC CGGTCATGAT GCTGAGCTCA GTGCCAGAAC TACACGGCTA CATTGATAAG 
TCACAGCTGA CCGAGGACCT CGGGGGGACC CTGGACTACT GCCATTCCAG GTGGCTGTGC 
CACCGCACGG CGATCGAAAG CTTCGCCCTG ATGGTGAAGC AGACAGCTCA GATGCTGCAG 
GCCTTCGGGA CCGAGCTGGC CGAAACAGAA CTGCCCAACG ATGTCCAATC AACAAGCTCG 
GTGCTGAGCA CACACACGGA GAAGAAGGAC AAAGCAAAGG AGGATCTGCA GCTGGCACGG 
AAAGAGGGGA ACAGCATCCT TGAGAGCCTC AGGGAGCCAC TGGCTGAAAG CACTGCCCAC 
AGTGTAAACC AAGACCAGCT GGACAATCAG GCCACAGTGC AGAGGCTCCT GGCTCAGCTG 
AATGAAACGG AGGCCGCCTT TGACGAGTTC TGGGCAAAGC ATCAGCAGAA GCTGGAACAG 
TGCCTGCAGC TGCGGCATTT TGAGCAGGGT TTCCGGGAGG TCAAAACTAC CTTGGATACC 
ATGTCCCACA AGATAGCCAC CTTCACTGAC GTTGGTAACA GCCTGGCACA TGTGCAACAC 
CTGCTGAAGG ACCTGGCCAG CTTCGAAGAG AAGTCTAGCG TGGCTGTAGA CAGGGCCCGA 
GCCCTGTCAC TGGAGGGTCA GCAGCTGATT GAGAACAAGC ACTATGCTGT GGACTCCATC 
CACCCTAAGT GTGAGGAGCT CCAGCACCTC TGCGATCACT TCACCGCTGA GATCAGCAGG 
AGGAGAGGCT TGCTCAGCAA GTCCCTGGAG CTACACAGCC TCTTGGAGAC GTCCATGAAG 
TGGTGCGATG AGGGCATCTT CCTGCTGGCC TCGCAACCTG TGGACAAGTG TCAGTCCCAG 
GATGGTGCTG AGGCAGCCCT CCAGGAGATC GAGAAATTTT TGGAGACTGG TGCAGAAAAT 
AAGATTCAAG AACTCAATAA AATTTACAAG GAATATGAGT GCATCCTCAA CCAGGACCTA 
CTGGAACACG TGCAAAAGGT CTTTCAGAAG CAAGAAAGTA TGCAAGAGAT GTTCCACCGC 
AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC AAGCAGACAC GGCCTGTGCA GCCCGTGGCC 
CCACGGCCAG AGGCACTCAC CAAATCACCC TGCCCCTCCC CAGGCTCCTG GAGAGGATCT 
GAGAACTCTA GCTCTGAGGG CAGTGCACTC CGTAGAGGGC CCTACAGGAG AGCCAAGAGT 
GAAATGAGTG AGACCCGGCA GGGCCGTGCC AGCTCCACAG GGGACGAGGA GGAGAGCCTA 
GCCATCCTGC GCAGGCATGT GATGAATGAG CTCCTAGACA CGGAACGGGC CTACGTGGAG 
GAGCTGCTCT GTGTACTAGA GGGCTATGCT GCAGAGATGG ACAACCCCCT GATGGCACAC 
CTCATTTCAG CAGGCCTGCA AAACAAGAAG AACATTCTGT TTGGAAACAT GGAGGAGATT 
TATCACTTTC ATAACAGGAT ATTCCTGCGG GAGCTAGAGA GCTGCATAGA CTGCCCGGAA 
CTGGTGGGGA GGTGCTTTCT GGAGAGGATG GAGGAGTTCC AGATCTACGA GAAGTACTGT 
CAGAACAAGC CACGATCTGA GAGCCTGTGG AGACAGTGCT CTGACTGTCC CTTCTTCCAG 
GAGTGCCAGA AGAAGTTGGA CCACAAACTG AGCCTTGATT CCTACCTGCT GAAGCCAGTG 
CAGAGAATAA CTAAGTACCA GTTGCTGCTT AAGGAAATGT TGAAATACAG CAAGAACTGT 
GAGGGGGCAG AGGACCTACA GGAGGCACTG AGCTCCATCC TGGGCATCCT CAAGGCAGTG 
AATGACTCCA TGCACCTCAT CGCCATCACT GGCTACGACG GTAACCTGGG TGATCTGGGG 
AAGCTGCTGA TGCAAGGTTC CTTCAGTGTA TGGACAGACC ACAAGAAGGG CCACACCAAG 
GTCAAGGAGC TGGCCAGATT CAAGCCCATG CAGCGGCACC TTTTCCTGCA TGAGAAGGCT 
GTGCTGTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAGGC CCCTTCCTAC 
AGCTACAAGC AGTCATTGAA TATGACTGCT GTTGGCATCA CAGAGAATGT GAAGGGTGAT 
GCCAAGAAGT TCGAGATCTG GTACAACGCA AGAGAGGAGG TTTACATCAT CCAGGCACCA 
ACCCCTGAGA TTAAAGCAGC ATGGGTGAGT GAGATTCGGA AGGTGCTGAC CAGCCAGCTG 
CAGGCCTGCC GGGAAGCCAG CCAGCACAGG GCCCTGGAGC AGTCCCACAG CCTGCCTTTG 
CCCACACCAT CCAACACCAG CCCCTCAAAA GGGAACACAA GGAATGTCAA GAAGTCAGAA 
GAAAGAAAAA CGGACCCCCT GAGCCTGGAA GGATATGTGA GCTCATCGTT GCCGAAGCCC 
CCTGAGAAAG GCAAAGACGA TGCGGTCCCT AGTTCTACCT CTGAAAGCTC TGCGCTTTCC 
AGAAAGCGCT TCACGCTGCA GGGCTTGGCT AACCTCAAAG GTCAGAAAGC CTCTCCGACC 
AGTCCTGACA AAAAAGCTAA GCGGCACGAA GTAAAGAGCG ACCCGACTCC CTTTGGTTTG 
CGAGGCTGGA GCAAAACATC CCACTCCCTG GAAGCCCCTG AGGAGGACGG AGGTTGGTCC 
AGTGCTGAGG AGCTTGTCAA TTCATCTGAT GCAGAGGAAG ATGGAGTGGG CCCCAAGAAG 
CTGGTTCCTG GTAAATACAC AGTGGTGATG GACGATGAGA AAGGGGGCCT TGACACCCTT 
GCTGTGAGGA GCGGGGACAT GGTAGAGGTG GTGGAAGAGG GCGCAGAGGG GCTCTGGTAT 
GTCCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT TTCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC 
GTGCTGAGCA ACTCCTCCAG CTGCAGCGAG GGCTGCCCCA CTCCCTTCTC CGACCTCCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_029553704.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029409564.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X16, mRNA.

Price & Availability↑

CloneID	ONa135172
Clone ID Related Accession (Same CDS sequence)	XM_029553701.1 , XM_029553703.1 , XM_029553702.1 , XM_029553704.1
Accession Version	XM_029553704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTGC CGGTCATGAT GCTGAGCTCA GTGCCAGAAC TACACGGCTA CATTGATAAG 
TCACAGCTGA CCGAGGACCT CGGGGGGACC CTGGACTACT GCCATTCCAG GTGGCTGTGC 
CACCGCACGG CGATCGAAAG CTTCGCCCTG ATGGTGAAGC AGACAGCTCA GATGCTGCAG 
GCCTTCGGGA CCGAGCTGGC CGAAACAGAA CTGCCCAACG ATGTCCAATC AACAAGCTCG 
GTGCTGAGCA CACACACGGA GAAGAAGGAC AAAGCAAAGG AGGATCTGCA GCTGGCACGG 
AAAGAGGGGA ACAGCATCCT TGAGAGCCTC AGGGAGCCAC TGGCTGAAAG CACTGCCCAC 
AGTGTAAACC AAGACCAGCT GGACAATCAG GCCACAGTGC AGAGGCTCCT GGCTCAGCTG 
AATGAAACGG AGGCCGCCTT TGACGAGTTC TGGGCAAAGC ATCAGCAGAA GCTGGAACAG 
TGCCTGCAGC TGCGGCATTT TGAGCAGGGT TTCCGGGAGG TCAAAACTAC CTTGGATACC 
ATGTCCCACA AGATAGCCAC CTTCACTGAC GTTGGTAACA GCCTGGCACA TGTGCAACAC 
CTGCTGAAGG ACCTGGCCAG CTTCGAAGAG AAGTCTAGCG TGGCTGTAGA CAGGGCCCGA 
GCCCTGTCAC TGGAGGGTCA GCAGCTGATT GAGAACAAGC ACTATGCTGT GGACTCCATC 
CACCCTAAGT GTGAGGAGCT CCAGCACCTC TGCGATCACT TCACCGCTGA GATCAGCAGG 
AGGAGAGGCT TGCTCAGCAA GTCCCTGGAG CTACACAGCC TCTTGGAGAC GTCCATGAAG 
TGGTGCGATG AGGGCATCTT CCTGCTGGCC TCGCAACCTG TGGACAAGTG TCAGTCCCAG 
GATGGTGCTG AGGCAGCCCT CCAGGAGATC GAGAAATTTT TGGAGACTGG TGCAGAAAAT 
AAGATTCAAG AACTCAATAA AATTTACAAG GAATATGAGT GCATCCTCAA CCAGGACCTA 
CTGGAACACG TGCAAAAGGT CTTTCAGAAG CAAGAAAGTA TGCAAGAGAT GTTCCACCGC 
AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC AAGCAGACAC GGCCTGTGCA GCCCGTGGCC 
CCACGGCCAG AGGCACTCAC CAAATCACCC TGCCCCTCCC CAGGCTCCTG GAGAGGATCT 
GAGAACTCTA GCTCTGAGGG CAGTGCACTC CGTAGAGGGC CCTACAGGAG AGCCAAGAGT 
GAAATGAGTG AGACCCGGCA GGGCCGTGCC AGCTCCACAG GGGACGAGGA GGAGAGCCTA 
GCCATCCTGC GCAGGCATGT GATGAATGAG CTCCTAGACA CGGAACGGGC CTACGTGGAG 
GAGCTGCTCT GTGTACTAGA GGGCTATGCT GCAGAGATGG ACAACCCCCT GATGGCACAC 
CTCATTTCAG CAGGCCTGCA AAACAAGAAG AACATTCTGT TTGGAAACAT GGAGGAGATT 
TATCACTTTC ATAACAGGAT ATTCCTGCGG GAGCTAGAGA GCTGCATAGA CTGCCCGGAA 
CTGGTGGGGA GGTGCTTTCT GGAGAGGATG GAGGAGTTCC AGATCTACGA GAAGTACTGT 
CAGAACAAGC CACGATCTGA GAGCCTGTGG AGACAGTGCT CTGACTGTCC CTTCTTCCAG 
GAGTGCCAGA AGAAGTTGGA CCACAAACTG AGCCTTGATT CCTACCTGCT GAAGCCAGTG 
CAGAGAATAA CTAAGTACCA GTTGCTGCTT AAGGAAATGT TGAAATACAG CAAGAACTGT 
GAGGGGGCAG AGGACCTACA GGAGGCACTG AGCTCCATCC TGGGCATCCT CAAGGCAGTG 
AATGACTCCA TGCACCTCAT CGCCATCACT GGCTACGACG GTAACCTGGG TGATCTGGGG 
AAGCTGCTGA TGCAAGGTTC CTTCAGTGTA TGGACAGACC ACAAGAAGGG CCACACCAAG 
GTCAAGGAGC TGGCCAGATT CAAGCCCATG CAGCGGCACC TTTTCCTGCA TGAGAAGGCT 
GTGCTGTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAGGC CCCTTCCTAC 
AGCTACAAGC AGTCATTGAA TATGACTGCT GTTGGCATCA CAGAGAATGT GAAGGGTGAT 
GCCAAGAAGT TCGAGATCTG GTACAACGCA AGAGAGGAGG TTTACATCAT CCAGGCACCA 
ACCCCTGAGA TTAAAGCAGC ATGGGTGAGT GAGATTCGGA AGGTGCTGAC CAGCCAGCTG 
CAGGCCTGCC GGGAAGCCAG CCAGCACAGG GCCCTGGAGC AGTCCCACAG CCTGCCTTTG 
CCCACACCAT CCAACACCAG CCCCTCAAAA GGGAACACAA GGAATGTCAA GAAGTCAGAA 
GAAAGAAAAA CGGACCCCCT GAGCCTGGAA GGATATGTGA GCTCATCGTT GCCGAAGCCC 
CCTGAGAAAG GCAAAGACGA TGCGGTCCCT AGTTCTACCT CTGAAAGCTC TGCGCTTTCC 
AGAAAGCGCT TCACGCTGCA GGGCTTGGCT AACCTCAAAG GTCAGAAAGC CTCTCCGACC 
AGTCCTGACA AAAAAGCTAA GCGGCACGAA GTAAAGAGCG ACCCGACTCC CTTTGGTTTG 
CGAGGCTGGA GCAAAACATC CCACTCCCTG GAAGCCCCTG AGGAGGACGG AGGTTGGTCC 
AGTGCTGAGG AGCTTGTCAA TTCATCTGAT GCAGAGGAAG ATGGAGTGGG CCCCAAGAAG 
CTGGTTCCTG GTAAATACAC AGTGGTGATG GACGATGAGA AAGGGGGCCT TGACACCCTT 
GCTGTGAGGA GCGGGGACAT GGTAGAGGTG GTGGAAGAGG GCGCAGAGGG GCTCTGGTAT 
GTCCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT TTCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC 
GTGCTGAGCA ACTCCTCCAG CTGCAGCGAG GGCTGCCCCA CTCCCTTCTC CGACCTCCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029409564.1
CDS	247..3312
Translation	MKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_029553704.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029553704.1

ORF Insert Sequence:

ATGAAGGTGC CGGTCATGAT GCTGAGCTCA GTGCCAGAAC TACACGGCTA CATTGATAAG 
TCACAGCTGA CCGAGGACCT CGGGGGGACC CTGGACTACT GCCATTCCAG GTGGCTGTGC 
CACCGCACGG CGATCGAAAG CTTCGCCCTG ATGGTGAAGC AGACAGCTCA GATGCTGCAG 
GCCTTCGGGA CCGAGCTGGC CGAAACAGAA CTGCCCAACG ATGTCCAATC AACAAGCTCG 
GTGCTGAGCA CACACACGGA GAAGAAGGAC AAAGCAAAGG AGGATCTGCA GCTGGCACGG 
AAAGAGGGGA ACAGCATCCT TGAGAGCCTC AGGGAGCCAC TGGCTGAAAG CACTGCCCAC 
AGTGTAAACC AAGACCAGCT GGACAATCAG GCCACAGTGC AGAGGCTCCT GGCTCAGCTG 
AATGAAACGG AGGCCGCCTT TGACGAGTTC TGGGCAAAGC ATCAGCAGAA GCTGGAACAG 
TGCCTGCAGC TGCGGCATTT TGAGCAGGGT TTCCGGGAGG TCAAAACTAC CTTGGATACC 
ATGTCCCACA AGATAGCCAC CTTCACTGAC GTTGGTAACA GCCTGGCACA TGTGCAACAC 
CTGCTGAAGG ACCTGGCCAG CTTCGAAGAG AAGTCTAGCG TGGCTGTAGA CAGGGCCCGA 
GCCCTGTCAC TGGAGGGTCA GCAGCTGATT GAGAACAAGC ACTATGCTGT GGACTCCATC 
CACCCTAAGT GTGAGGAGCT CCAGCACCTC TGCGATCACT TCACCGCTGA GATCAGCAGG 
AGGAGAGGCT TGCTCAGCAA GTCCCTGGAG CTACACAGCC TCTTGGAGAC GTCCATGAAG 
TGGTGCGATG AGGGCATCTT CCTGCTGGCC TCGCAACCTG TGGACAAGTG TCAGTCCCAG 
GATGGTGCTG AGGCAGCCCT CCAGGAGATC GAGAAATTTT TGGAGACTGG TGCAGAAAAT 
AAGATTCAAG AACTCAATAA AATTTACAAG GAATATGAGT GCATCCTCAA CCAGGACCTA 
CTGGAACACG TGCAAAAGGT CTTTCAGAAG CAAGAAAGTA TGCAAGAGAT GTTCCACCGC 
AGGCAGGCCA GCCTGAAGAA GCTGGCGGCC AAGCAGACAC GGCCTGTGCA GCCCGTGGCC 
CCACGGCCAG AGGCACTCAC CAAATCACCC TGCCCCTCCC CAGGCTCCTG GAGAGGATCT 
GAGAACTCTA GCTCTGAGGG CAGTGCACTC CGTAGAGGGC CCTACAGGAG AGCCAAGAGT 
GAAATGAGTG AGACCCGGCA GGGCCGTGCC AGCTCCACAG GGGACGAGGA GGAGAGCCTA 
GCCATCCTGC GCAGGCATGT GATGAATGAG CTCCTAGACA CGGAACGGGC CTACGTGGAG 
GAGCTGCTCT GTGTACTAGA GGGCTATGCT GCAGAGATGG ACAACCCCCT GATGGCACAC 
CTCATTTCAG CAGGCCTGCA AAACAAGAAG AACATTCTGT TTGGAAACAT GGAGGAGATT 
TATCACTTTC ATAACAGGAT ATTCCTGCGG GAGCTAGAGA GCTGCATAGA CTGCCCGGAA 
CTGGTGGGGA GGTGCTTTCT GGAGAGGATG GAGGAGTTCC AGATCTACGA GAAGTACTGT 
CAGAACAAGC CACGATCTGA GAGCCTGTGG AGACAGTGCT CTGACTGTCC CTTCTTCCAG 
GAGTGCCAGA AGAAGTTGGA CCACAAACTG AGCCTTGATT CCTACCTGCT GAAGCCAGTG 
CAGAGAATAA CTAAGTACCA GTTGCTGCTT AAGGAAATGT TGAAATACAG CAAGAACTGT 
GAGGGGGCAG AGGACCTACA GGAGGCACTG AGCTCCATCC TGGGCATCCT CAAGGCAGTG 
AATGACTCCA TGCACCTCAT CGCCATCACT GGCTACGACG GTAACCTGGG TGATCTGGGG 
AAGCTGCTGA TGCAAGGTTC CTTCAGTGTA TGGACAGACC ACAAGAAGGG CCACACCAAG 
GTCAAGGAGC TGGCCAGATT CAAGCCCATG CAGCGGCACC TTTTCCTGCA TGAGAAGGCT 
GTGCTGTTCT GCAAGAAGAG GGAGGAGAAT GGGGAGGGGT ATGAGAAGGC CCCTTCCTAC 
AGCTACAAGC AGTCATTGAA TATGACTGCT GTTGGCATCA CAGAGAATGT GAAGGGTGAT 
GCCAAGAAGT TCGAGATCTG GTACAACGCA AGAGAGGAGG TTTACATCAT CCAGGCACCA 
ACCCCTGAGA TTAAAGCAGC ATGGGTGAGT GAGATTCGGA AGGTGCTGAC CAGCCAGCTG 
CAGGCCTGCC GGGAAGCCAG CCAGCACAGG GCCCTGGAGC AGTCCCACAG CCTGCCTTTG 
CCCACACCAT CCAACACCAG CCCCTCAAAA GGGAACACAA GGAATGTCAA GAAGTCAGAA 
GAAAGAAAAA CGGACCCCCT GAGCCTGGAA GGATATGTGA GCTCATCGTT GCCGAAGCCC 
CCTGAGAAAG GCAAAGACGA TGCGGTCCCT AGTTCTACCT CTGAAAGCTC TGCGCTTTCC 
AGAAAGCGCT TCACGCTGCA GGGCTTGGCT AACCTCAAAG GTCAGAAAGC CTCTCCGACC 
AGTCCTGACA AAAAAGCTAA GCGGCACGAA GTAAAGAGCG ACCCGACTCC CTTTGGTTTG 
CGAGGCTGGA GCAAAACATC CCACTCCCTG GAAGCCCCTG AGGAGGACGG AGGTTGGTCC 
AGTGCTGAGG AGCTTGTCAA TTCATCTGAT GCAGAGGAAG ATGGAGTGGG CCCCAAGAAG 
CTGGTTCCTG GTAAATACAC AGTGGTGATG GACGATGAGA AAGGGGGCCT TGACACCCTT 
GCTGTGAGGA GCGGGGACAT GGTAGAGGTG GTGGAAGAGG GCGCAGAGGG GCTCTGGTAT 
GTCCGGGACC TGACCAGCAG CAAGGAGGGA TGGGTGCCAG CCAGCAGCCT TTCCACCCTC 
CTGGGCAAAT CCAGTTCAGC CCAGTGTCTG AGCAGCTCAG ACTCCAGCCC AGGGTCAGCC 
GTGCTGAGCA ACTCCTCCAG CTGCAGCGAG GGCTGCCCCA CTCCCTTCTC CGACCTCCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_029553705.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029409565.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X17, mRNA.

Price & Availability↑

CloneID	ONa135173
Clone ID Related Accession (Same CDS sequence)	XM_029553705.1
Accession Version	XM_029553705.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTAT CCTGGAGGGC GAAAACTCAT GCCCCACACC CCACAGACGC CAGCGAGCCT 
TCAGAAGCCT TGGGGATTTT TACTCAAAGT TGTAATGATG TCATCAGGAG CCATTTTTTA 
ACGTGGATGA ACACGGGGAC CTCAGATACT AGGATAACTT TGCAAGATGC TGGCATTGGA 
TTCATTCTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CCGTGAAGGC GTCGGTCCTG 
CGAATAGCCG CATCTTTCCC AGCAAACCTG CAGCTCGTCC TTGTGCTTCG CCCAACGGGC 
TTTTTCCAGA GGACTCTCTC TGACCTCGCC TTCAAGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATGATGCT GAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCA 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ATTCCAGGTG GCTGTGCCAC 
CGCACGGCGA TCGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCTCAGAT GCTGCAGGCC 
TTCGGGACCG AGCTGGCCGA AACAGAACTG CCCAACGATG TCCAATCAAC AAGCTCGGTG 
CTGAGCACAC ACACGGAGAA GAAGGACAAA GCAAAGGAGG ATCTGCAGCT GGCACGGAAA 
GAGGGGAACA GCATCCTTGA GAGCCTCAGG GAGCCACTGG CTGAAAGCAC TGCCCACAGT 
GTAAACCAAG ACCAGCTGGA CAATCAGGCC ACAGTGCAGA GGCTCCTGGC TCAGCTGAAT 
GAAACGGAGG CCGCCTTTGA CGAGTTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAGGGTTTC CGGGAGGTCA AAACTACCTT GGATACCATG 
TCCCACAAGA TAGCCACCTT CACTGACGTT GGTAACAGCC TGGCACATGT GCAACACCTG 
CTGAAGGACC TGGCCAGCTT CGAAGAGAAG TCTAGCGTGG CTGTAGACAG GGCCCGAGCC 
CTGTCACTGG AGGGTCAGCA GCTGATTGAG AACAAGCACT ATGCTGTGGA CTCCATCCAC 
CCTAAGTGTG AGGAGCTCCA GCACCTCTGC GATCACTTCA CCGCTGAGAT CAGCAGGAGG 
AGAGGCTTGC TCAGCAAGTC CCTGGAGCTA CACAGCCTCT TGGAGACGTC CATGAAGTGG 
TGCGATGAGG GCATCTTCCT GCTGGCCTCG CAACCTGTGG ACAAGTGTCA GTCCCAGGAT 
GGTGCTGAGG CAGCCCTCCA GGAGATCGAG AAATTTTTGG AGACTGGTGC AGAAAATAAG 
ATTCAAGAAC TCAATAAAAT TTACAAGGAA TATGAGTGCA TCCTCAACCA GGACCTACTG 
GAACACGTGC AAAAGGTCTT TCAGAAGCAA GAAAGTATGC AAGAGATGTT CCACCGCAGG 
CAGGCCAGCC TGAAGAAGCT GGCGGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCA 
CGGCCAGAGG CACTCACCAA ATCACCCTGC CCCTCCCCAG GCTCCTGGAG AGGATCTGAG 
AACTCTAGCT CTGAGGGCAG TGCACTCCGT AGAGGGCCCT ACAGGAGAGC CAAGAGTGAA 
ATGAGTGAGA CCCGGCAGGG CCGTGCCAGC TCCACAGGGG ACGAGGAGGA GAGCCTAGCC 
ATCCTGCGCA GGCATGTGAT GAATGAGCTC CTAGACACGG AACGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TACTAGAGGG CTATGCTGCA GAGATGGACA ACCCCCTGAT GGCACACCTC 
ATTTCAGCAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAGATTTAT 
CACTTTCATA ACAGGATATT CCTGCGGGAG CTAGAGAGCT GCATAGACTG CCCGGAACTG 
GTGGGGAGGT GCTTTCTGGA GAGGATGGAG GAGTTCCAGA TCTACGAGAA GTACTGTCAG 
AACAAGCCAC GATCTGAGAG CCTGTGGAGA CAGTGCTCTG ACTGTCCCTT CTTCCAGGAG 
TGCCAGAAGA AGTTGGACCA CAAACTGAGC CTTGATTCCT ACCTGCTGAA GCCAGTGCAG 
AGAATAACTA AGTACCAGTT GCTGCTTAAG GAAATGTTGA AATACAGCAA GAACTGTGAG 
GGGGCAGAGG ACCTACAGGA GGCACTGAGC TCCATCCTGG GCATCCTCAA GGCAGTGAAT 
GACTCCATGC ACCTCATCGC CATCACTGGC TACGACGGTA ACCTGGGTGA TCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGTGTATGG ACAGACCACA AGAAGGGCCA CACCAAGGTC 
AAGGAGCTGG CCAGATTCAA GCCCATGCAG CGGCACCTTT TCCTGCATGA GAAGGCTGTG 
CTGTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGGTATG AGAAGGCCCC TTCCTACAGC 
TACAAGCAGT CATTGAATAT GACTGCTGTT GGCATCACAG AGAATGTGAA GGGTGATGCC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGA GAGGAGGTTT ACATCATCCA GGCACCAACC 
CCTGAGATTA AAGCAGCATG GGTGAGTGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCCTGCCGGG AAGCCAGCCA GCACAGGGCC CTGGAGCAGT CCCACAGCCT GCCTTTGCCC 
ACACCATCCA ACACCAGCCC CTCAAAAGGG AACACAAGGA ATGTCAAGAA GTCAGAAGAA 
AGAAAAACGG ACCCCCTGAG CCTGGAAGGA TATGTGAGCT CATCGTTGCC GAAGCCCCCT 
GAGAAAGGCA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAAGCC 
CCTGAGGAGG ACGGAGGTTG GTCCAGTGCT GAGGAGCTTG TCAATTCATC TGATGCAGAG 
GAAGATGGAG TGGGCCCCAA GAAGCTGGTT CCTGGTAAAT ACACAGTGGT GATGGACGAT 
GAGAAAGGGG GCCTTGACAC CCTTGCTGTG AGGAGCGGGG ACATGGTAGA GGTGGTGGAA 
GAGGGCGCAG AGGGGCTCTG GTATGTCCGG GACCTGACCA GCAGCAAGGA GGGATGGGTG 
CCAGCCAGCA GCCTTTCCAC CCTCCTGGGC AAATCCAGTT CAGCCCAGTG TCTGAGCAGC 
TCAGACTCCA GCCCAGGGTC AGCCGTGCTG AGCAACTCCT CCAGCTGCAG CGAGGGCTGC 
CCCACTCCCT TCTCCGACCT CCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029409565.1
CDS	51..3380
Translation	MSLSWRAKTHAPHPTDASEPSEALGIFTQSCNDVIRSHFLTWMNTGTSDTRITLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_029553705.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029553705.1

ORF Insert Sequence:

ATGTCGCTAT CCTGGAGGGC GAAAACTCAT GCCCCACACC CCACAGACGC CAGCGAGCCT 
TCAGAAGCCT TGGGGATTTT TACTCAAAGT TGTAATGATG TCATCAGGAG CCATTTTTTA 
ACGTGGATGA ACACGGGGAC CTCAGATACT AGGATAACTT TGCAAGATGC TGGCATTGGA 
TTCATTCTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CCGTGAAGGC GTCGGTCCTG 
CGAATAGCCG CATCTTTCCC AGCAAACCTG CAGCTCGTCC TTGTGCTTCG CCCAACGGGC 
TTTTTCCAGA GGACTCTCTC TGACCTCGCC TTCAAGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATGATGCT GAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCA 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ATTCCAGGTG GCTGTGCCAC 
CGCACGGCGA TCGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCTCAGAT GCTGCAGGCC 
TTCGGGACCG AGCTGGCCGA AACAGAACTG CCCAACGATG TCCAATCAAC AAGCTCGGTG 
CTGAGCACAC ACACGGAGAA GAAGGACAAA GCAAAGGAGG ATCTGCAGCT GGCACGGAAA 
GAGGGGAACA GCATCCTTGA GAGCCTCAGG GAGCCACTGG CTGAAAGCAC TGCCCACAGT 
GTAAACCAAG ACCAGCTGGA CAATCAGGCC ACAGTGCAGA GGCTCCTGGC TCAGCTGAAT 
GAAACGGAGG CCGCCTTTGA CGAGTTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAGGGTTTC CGGGAGGTCA AAACTACCTT GGATACCATG 
TCCCACAAGA TAGCCACCTT CACTGACGTT GGTAACAGCC TGGCACATGT GCAACACCTG 
CTGAAGGACC TGGCCAGCTT CGAAGAGAAG TCTAGCGTGG CTGTAGACAG GGCCCGAGCC 
CTGTCACTGG AGGGTCAGCA GCTGATTGAG AACAAGCACT ATGCTGTGGA CTCCATCCAC 
CCTAAGTGTG AGGAGCTCCA GCACCTCTGC GATCACTTCA CCGCTGAGAT CAGCAGGAGG 
AGAGGCTTGC TCAGCAAGTC CCTGGAGCTA CACAGCCTCT TGGAGACGTC CATGAAGTGG 
TGCGATGAGG GCATCTTCCT GCTGGCCTCG CAACCTGTGG ACAAGTGTCA GTCCCAGGAT 
GGTGCTGAGG CAGCCCTCCA GGAGATCGAG AAATTTTTGG AGACTGGTGC AGAAAATAAG 
ATTCAAGAAC TCAATAAAAT TTACAAGGAA TATGAGTGCA TCCTCAACCA GGACCTACTG 
GAACACGTGC AAAAGGTCTT TCAGAAGCAA GAAAGTATGC AAGAGATGTT CCACCGCAGG 
CAGGCCAGCC TGAAGAAGCT GGCGGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCA 
CGGCCAGAGG CACTCACCAA ATCACCCTGC CCCTCCCCAG GCTCCTGGAG AGGATCTGAG 
AACTCTAGCT CTGAGGGCAG TGCACTCCGT AGAGGGCCCT ACAGGAGAGC CAAGAGTGAA 
ATGAGTGAGA CCCGGCAGGG CCGTGCCAGC TCCACAGGGG ACGAGGAGGA GAGCCTAGCC 
ATCCTGCGCA GGCATGTGAT GAATGAGCTC CTAGACACGG AACGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TACTAGAGGG CTATGCTGCA GAGATGGACA ACCCCCTGAT GGCACACCTC 
ATTTCAGCAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAGATTTAT 
CACTTTCATA ACAGGATATT CCTGCGGGAG CTAGAGAGCT GCATAGACTG CCCGGAACTG 
GTGGGGAGGT GCTTTCTGGA GAGGATGGAG GAGTTCCAGA TCTACGAGAA GTACTGTCAG 
AACAAGCCAC GATCTGAGAG CCTGTGGAGA CAGTGCTCTG ACTGTCCCTT CTTCCAGGAG 
TGCCAGAAGA AGTTGGACCA CAAACTGAGC CTTGATTCCT ACCTGCTGAA GCCAGTGCAG 
AGAATAACTA AGTACCAGTT GCTGCTTAAG GAAATGTTGA AATACAGCAA GAACTGTGAG 
GGGGCAGAGG ACCTACAGGA GGCACTGAGC TCCATCCTGG GCATCCTCAA GGCAGTGAAT 
GACTCCATGC ACCTCATCGC CATCACTGGC TACGACGGTA ACCTGGGTGA TCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGTGTATGG ACAGACCACA AGAAGGGCCA CACCAAGGTC 
AAGGAGCTGG CCAGATTCAA GCCCATGCAG CGGCACCTTT TCCTGCATGA GAAGGCTGTG 
CTGTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGGTATG AGAAGGCCCC TTCCTACAGC 
TACAAGCAGT CATTGAATAT GACTGCTGTT GGCATCACAG AGAATGTGAA GGGTGATGCC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGA GAGGAGGTTT ACATCATCCA GGCACCAACC 
CCTGAGATTA AAGCAGCATG GGTGAGTGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCCTGCCGGG AAGCCAGCCA GCACAGGGCC CTGGAGCAGT CCCACAGCCT GCCTTTGCCC 
ACACCATCCA ACACCAGCCC CTCAAAAGGG AACACAAGGA ATGTCAAGAA GTCAGAAGAA 
AGAAAAACGG ACCCCCTGAG CCTGGAAGGA TATGTGAGCT CATCGTTGCC GAAGCCCCCT 
GAGAAAGGCA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAAGCC 
CCTGAGGAGG ACGGAGGTTG GTCCAGTGCT GAGGAGCTTG TCAATTCATC TGATGCAGAG 
GAAGATGGAG TGGGCCCCAA GAAGCTGGTT CCTGGTAAAT ACACAGTGGT GATGGACGAT 
GAGAAAGGGG GCCTTGACAC CCTTGCTGTG AGGAGCGGGG ACATGGTAGA GGTGGTGGAA 
GAGGGCGCAG AGGGGCTCTG GTATGTCCGG GACCTGACCA GCAGCAAGGA GGGATGGGTG 
CCAGCCAGCA GCCTTTCCAC CCTCCTGGGC AAATCCAGTT CAGCCCAGTG TCTGAGCAGC 
TCAGACTCCA GCCCAGGGTC AGCCGTGCTG AGCAACTCCT CCAGCTGCAG CGAGGGCTGC 
CCCACTCCCT TCTCCGACCT CCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_029553706.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029409566.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X18, mRNA.

Price & Availability↑

CloneID	ONa135174
Clone ID Related Accession (Same CDS sequence)	XM_029553706.1
Accession Version	XM_029553706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTAT CCTGGAGGGC GAAAACTCAT GCCCCACACC CCACAGACGC CAGCGAGCCT 
TCAGAAGCCT TGGGGATTTT TACTCAAAGT TGTAATGATG TCATCAGGAG CCATTTTTTA 
ACGTGGATGA ACACGGGGAC CTCAGATACT AGGATAACTT TGCAAGATGC TGGCATTGGA 
TTCATTCTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CCGTGAAGGC GTCGGTCCTG 
CGAATAGCCG CATCTTTCCC AGCAAACCTG CAGCTCGTCC TTGTGCTTCG CCCAACGGGC 
TTTTTCCAGA GGACTCTCTC TGACCTCGCC TTCAAGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATGATGCT GAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCA 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ATTCCAGGTG GCTGTGCCAC 
CGCACGGCGA TCGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCTCAGAT GCTGCAGGCC 
TTCGGGACCG AGCTGGCCGA AACAGAACTG CCCAACGATG TCCAATCAAC AAGCTCGGTG 
CTGAGCACAC ACACGGAGAA GAAGGACAAA GCAAAGGAGG ATCTGCAGCT GGCACGGAAA 
GAGGGGAACA GCATCCTTGA GAGCCTCAGG GAGCCACTGG CTGAAAGCAC TGCCCACAGT 
GTAAACCAAG ACCAGCTGGA CAATCAGGCC ACAGTGCAGA GGCTCCTGGC TCAGCTGAAT 
GAAACGGAGG CCGCCTTTGA CGAGTTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAGGGTTTC CGGGAGGTCA AAACTACCTT GGATACCATG 
TCCCACAAGA TAGCCACCTT CACTGACGTT GGTAACAGCC TGGCACATGT GCAACACCTG 
CTGAAGGACC TGGCCAGCTT CGAAGAGAAG TCTAGCGTGG CTGTAGACAG GGCCCGAGCC 
CTGTCACTGG AGGGTCAGCA GCTGATTGAG AACAAGCACT ATGCTGTGGA CTCCATCCAC 
CCTAAGTGTG AGGAGCTCCA GCACCTCTGC GATCACTTCA CCGCTGAGAT CAGCAGGAGG 
AGAGGCTTGC TCAGCAAGTC CCTGGAGCTA CACAGCCTCT TGGAGACGTC CATGAAGTGG 
TGCGATGAGG GCATCTTCCT GCTGGCCTCG CAACCTGTGG ACAAGTGTCA GTCCCAGGAT 
GGTGCTGAGG CAGCCCTCCA GGAGATCGAG AAATTTTTGG AGACTGGTGC AGAAAATAAG 
ATTCAAGAAC TCAATAAAAT TTACAAGGAA TATGAGTGCA TCCTCAACCA GGACCTACTG 
GAACACGTGC AAAAGGTCTT TCAGAAGCAA GAAAGTATGC AAGAGATGTT CCACCGCAGG 
CAGGCCAGCC TGAAGAAGCT GGCGGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCA 
CGGCCAGAGG CACTCACCAA ATCACCCTGC CCCTCCCCAG GCTCCTGGAG AGGATCTGAG 
AACTCTAGCT CTGAGGGCAG TGCACTCCGT AGAGGGCCCT ACAGGAGAGC CAAGAGTGAA 
ATGAGTGAGA CCCGGCAGGG CCGTGCCAGC TCCACAGGGG ACGAGGAGGA GAGCCTAGCC 
ATCCTGCGCA GGCATGTGAT GAATGAGCTC CTAGACACGG AACGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TACTAGAGGG CTATGCTGCA GAGATGGACA ACCCCCTGAT GGCACACCTC 
ATTTCAGCAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAGATTTAT 
CACTTTCATA ACAGGATATT CCTGCGGGAG CTAGAGAGCT GCATAGACTG CCCGGAACTG 
GTGGGGAGGT GCTTTCTGGA GAGGATGGAG GAGTTCCAGA TCTACGAGAA GTACTGTCAG 
AACAAGCCAC GATCTGAGAG CCTGTGGAGA CAGTGCTCTG ACTGTCCCTT CTTCCAGGAG 
TGCCAGAAGA AGTTGGACCA CAAACTGAGC CTTGATTCCT ACCTGCTGAA GCCAGTGCAG 
AGAATAACTA AGTACCAGTT GCTGCTTAAG GAAATGTTGA AATACAGCAA GAACTGTGAG 
GGGGCAGAGG ACCTACAGGA GGCACTGAGC TCCATCCTGG GCATCCTCAA GGCAGTGAAT 
GACTCCATGC ACCTCATCGC CATCACTGGC TACGACGGTA ACCTGGGTGA TCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGTGTATGG ACAGACCACA AGAAGGGCCA CACCAAGGTC 
AAGGAGCTGG CCAGATTCAA GCCCATGCAG CGGCACCTTT TCCTGCATGA GAAGGCTGTG 
CTGTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGGTATG AGAAGGCCCC TTCCTACAGC 
TACAAGCAGT CATTGAATAT GACTGCTGTT GGCATCACAG AGAATGTGAA GGGTGATGCC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGA GAGGAGGTTT ACATCATCCA GGCACCAACC 
CCTGAGATTA AAGCAGCATG GGTGAGTGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCCTGCCGGG AAGCCAGCCA GCACAGGGCC CTGGAGCAGT CCCACAGCCT GCCTTTGCCC 
ACACCATCCA ACACCAGCCC CTCAAAAGGG AACACAAGGA ATGTCAAGAA GTCAGAAGAA 
AGAAAAACGG ACCCCCTGAG CCTGGAAGGA TATGTGAGCT CATCGTTGCC GAAGCCCCCT 
GAGAAAGGCA AAGGCTGGAG CAAAACATCC CACTCCCTGG AAGCCCCTGA GGAGGACGGA 
GGTTGGTCCA GTGCTGAGGA GCTTGTCAAT TCATCTGATG CAGAGGAAGA TGGAGTGGGC 
CCCAAGAAGC TGGTTCCTGG TAAATACACA GTGGTGATGG ACGATGAGAA AGGGGGCCTT 
GACACCCTTG CTGTGAGGAG CGGGGACATG GTAGAGGTGG TGGAAGAGGG CGCAGAGGGG 
CTCTGGTATG TCCGGGACCT GACCAGCAGC AAGGAGGGAT GGGTGCCAGC CAGCAGCCTT 
TCCACCCTCC TGGGCAAATC CAGTTCAGCC CAGTGTCTGA GCAGCTCAGA CTCCAGCCCA 
GGGTCAGCCG TGCTGAGCAA CTCCTCCAGC TGCAGCGAGG GCTGCCCCAC TCCCTTCTCC 
GACCTCCAGG GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029409566.1
CDS	51..3305
Translation	MSLSWRAKTHAPHPTDASEPSEALGIFTQSCNDVIRSHFLTWMNTGTSDTRITLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_029553706.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029553706.1

ORF Insert Sequence:

ATGTCGCTAT CCTGGAGGGC GAAAACTCAT GCCCCACACC CCACAGACGC CAGCGAGCCT 
TCAGAAGCCT TGGGGATTTT TACTCAAAGT TGTAATGATG TCATCAGGAG CCATTTTTTA 
ACGTGGATGA ACACGGGGAC CTCAGATACT AGGATAACTT TGCAAGATGC TGGCATTGGA 
TTCATTCTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CCGTGAAGGC GTCGGTCCTG 
CGAATAGCCG CATCTTTCCC AGCAAACCTG CAGCTCGTCC TTGTGCTTCG CCCAACGGGC 
TTTTTCCAGA GGACTCTCTC TGACCTCGCC TTCAAGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATGATGCT GAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCA 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ATTCCAGGTG GCTGTGCCAC 
CGCACGGCGA TCGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCTCAGAT GCTGCAGGCC 
TTCGGGACCG AGCTGGCCGA AACAGAACTG CCCAACGATG TCCAATCAAC AAGCTCGGTG 
CTGAGCACAC ACACGGAGAA GAAGGACAAA GCAAAGGAGG ATCTGCAGCT GGCACGGAAA 
GAGGGGAACA GCATCCTTGA GAGCCTCAGG GAGCCACTGG CTGAAAGCAC TGCCCACAGT 
GTAAACCAAG ACCAGCTGGA CAATCAGGCC ACAGTGCAGA GGCTCCTGGC TCAGCTGAAT 
GAAACGGAGG CCGCCTTTGA CGAGTTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAGGGTTTC CGGGAGGTCA AAACTACCTT GGATACCATG 
TCCCACAAGA TAGCCACCTT CACTGACGTT GGTAACAGCC TGGCACATGT GCAACACCTG 
CTGAAGGACC TGGCCAGCTT CGAAGAGAAG TCTAGCGTGG CTGTAGACAG GGCCCGAGCC 
CTGTCACTGG AGGGTCAGCA GCTGATTGAG AACAAGCACT ATGCTGTGGA CTCCATCCAC 
CCTAAGTGTG AGGAGCTCCA GCACCTCTGC GATCACTTCA CCGCTGAGAT CAGCAGGAGG 
AGAGGCTTGC TCAGCAAGTC CCTGGAGCTA CACAGCCTCT TGGAGACGTC CATGAAGTGG 
TGCGATGAGG GCATCTTCCT GCTGGCCTCG CAACCTGTGG ACAAGTGTCA GTCCCAGGAT 
GGTGCTGAGG CAGCCCTCCA GGAGATCGAG AAATTTTTGG AGACTGGTGC AGAAAATAAG 
ATTCAAGAAC TCAATAAAAT TTACAAGGAA TATGAGTGCA TCCTCAACCA GGACCTACTG 
GAACACGTGC AAAAGGTCTT TCAGAAGCAA GAAAGTATGC AAGAGATGTT CCACCGCAGG 
CAGGCCAGCC TGAAGAAGCT GGCGGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCA 
CGGCCAGAGG CACTCACCAA ATCACCCTGC CCCTCCCCAG GCTCCTGGAG AGGATCTGAG 
AACTCTAGCT CTGAGGGCAG TGCACTCCGT AGAGGGCCCT ACAGGAGAGC CAAGAGTGAA 
ATGAGTGAGA CCCGGCAGGG CCGTGCCAGC TCCACAGGGG ACGAGGAGGA GAGCCTAGCC 
ATCCTGCGCA GGCATGTGAT GAATGAGCTC CTAGACACGG AACGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TACTAGAGGG CTATGCTGCA GAGATGGACA ACCCCCTGAT GGCACACCTC 
ATTTCAGCAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAGATTTAT 
CACTTTCATA ACAGGATATT CCTGCGGGAG CTAGAGAGCT GCATAGACTG CCCGGAACTG 
GTGGGGAGGT GCTTTCTGGA GAGGATGGAG GAGTTCCAGA TCTACGAGAA GTACTGTCAG 
AACAAGCCAC GATCTGAGAG CCTGTGGAGA CAGTGCTCTG ACTGTCCCTT CTTCCAGGAG 
TGCCAGAAGA AGTTGGACCA CAAACTGAGC CTTGATTCCT ACCTGCTGAA GCCAGTGCAG 
AGAATAACTA AGTACCAGTT GCTGCTTAAG GAAATGTTGA AATACAGCAA GAACTGTGAG 
GGGGCAGAGG ACCTACAGGA GGCACTGAGC TCCATCCTGG GCATCCTCAA GGCAGTGAAT 
GACTCCATGC ACCTCATCGC CATCACTGGC TACGACGGTA ACCTGGGTGA TCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGTGTATGG ACAGACCACA AGAAGGGCCA CACCAAGGTC 
AAGGAGCTGG CCAGATTCAA GCCCATGCAG CGGCACCTTT TCCTGCATGA GAAGGCTGTG 
CTGTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGGTATG AGAAGGCCCC TTCCTACAGC 
TACAAGCAGT CATTGAATAT GACTGCTGTT GGCATCACAG AGAATGTGAA GGGTGATGCC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGA GAGGAGGTTT ACATCATCCA GGCACCAACC 
CCTGAGATTA AAGCAGCATG GGTGAGTGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCCTGCCGGG AAGCCAGCCA GCACAGGGCC CTGGAGCAGT CCCACAGCCT GCCTTTGCCC 
ACACCATCCA ACACCAGCCC CTCAAAAGGG AACACAAGGA ATGTCAAGAA GTCAGAAGAA 
AGAAAAACGG ACCCCCTGAG CCTGGAAGGA TATGTGAGCT CATCGTTGCC GAAGCCCCCT 
GAGAAAGGCA AAGGCTGGAG CAAAACATCC CACTCCCTGG AAGCCCCTGA GGAGGACGGA 
GGTTGGTCCA GTGCTGAGGA GCTTGTCAAT TCATCTGATG CAGAGGAAGA TGGAGTGGGC 
CCCAAGAAGC TGGTTCCTGG TAAATACACA GTGGTGATGG ACGATGAGAA AGGGGGCCTT 
GACACCCTTG CTGTGAGGAG CGGGGACATG GTAGAGGTGG TGGAAGAGGG CGCAGAGGGG 
CTCTGGTATG TCCGGGACCT GACCAGCAGC AAGGAGGGAT GGGTGCCAGC CAGCAGCCTT 
TCCACCCTCC TGGGCAAATC CAGTTCAGCC CAGTGTCTGA GCAGCTCAGA CTCCAGCCCA 
GGGTCAGCCG TGCTGAGCAA CTCCTCCAGC TGCAGCGAGG GCTGCCCCAC TCCCTTCTCC 
GACCTCCAGG GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848407.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846629.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X1, mRNA.

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CloneID	ONa31072
Clone ID Related Accession (Same CDS sequence)	XM_008848407.1 , XM_008848407.2 , XM_029553699.1
Accession Version	XM_008848407.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG ACGATGCGGT CCCTAGTTCT 
ACCTCTGAAA GCTCTGCGCT TTCCAGAAAG CGCTTCACGC TGCAGGGCTT GGCTAACCTC 
AAAGGTCAGA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAAGCC 
CCTGAGGAGG ACGGAGGTTG GTCCAGTGCT GAGGAGCTTG TCAATTCATC TGATGCAGAG 
GAAGATGGAG TGGGCCCCAA GAAGCTGGTT CCTGGTAAAT ACACAGTGGT GATGGACGAT 
GAGAAAGGGG GCCTTGACAC CCTTGCTGTG AGGAGCGGGG ACATGGTAGA GGTGGTGGAA 
GAGGGCGCAG AGGGGCTCTG GTATGTCCGG GACCTGACCA GCAGCAAGGA GGGATGGGTG 
CCAGCCAGCA GCCTTTCCAC CCTCCTGGGC AAATCCAGTT CAGCCCAGTG TCTGAGCAGC 
TCAGACTCCA GCCCAGGGTC AGCCGTGCTG AGCAACTCCT CCAGCTGCAG CGAGGGCTGC 
CCCACTCCCT TCTCCGACCT CCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846629.1
CDS	29..3658
Translation	MSDCWRFILCKDRGRDSPSPQEHNGVPKEEADHQVDVSDGIKLVPDKAEAMVTAPDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_008848407.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848407.1

ORF Insert Sequence:

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG ACGATGCGGT CCCTAGTTCT 
ACCTCTGAAA GCTCTGCGCT TTCCAGAAAG CGCTTCACGC TGCAGGGCTT GGCTAACCTC 
AAAGGTCAGA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAAGCC 
CCTGAGGAGG ACGGAGGTTG GTCCAGTGCT GAGGAGCTTG TCAATTCATC TGATGCAGAG 
GAAGATGGAG TGGGCCCCAA GAAGCTGGTT CCTGGTAAAT ACACAGTGGT GATGGACGAT 
GAGAAAGGGG GCCTTGACAC CCTTGCTGTG AGGAGCGGGG ACATGGTAGA GGTGGTGGAA 
GAGGGCGCAG AGGGGCTCTG GTATGTCCGG GACCTGACCA GCAGCAAGGA GGGATGGGTG 
CCAGCCAGCA GCCTTTCCAC CCTCCTGGGC AAATCCAGTT CAGCCCAGTG TCTGAGCAGC 
TCAGACTCCA GCCCAGGGTC AGCCGTGCTG AGCAACTCCT CCAGCTGCAG CGAGGGCTGC 
CCCACTCCCT TCTCCGACCT CCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848407.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846629.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONa31072
Clone ID Related Accession (Same CDS sequence)	XM_008848407.1 , XM_008848407.2 , XM_029553699.1
Accession Version	XM_008848407.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008848407.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG ACGATGCGGT CCCTAGTTCT 
ACCTCTGAAA GCTCTGCGCT TTCCAGAAAG CGCTTCACGC TGCAGGGCTT GGCTAACCTC 
AAAGGTCAGA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAAGCC 
CCTGAGGAGG ACGGAGGTTG GTCCAGTGCT GAGGAGCTTG TCAATTCATC TGATGCAGAG 
GAAGATGGAG TGGGCCCCAA GAAGCTGGTT CCTGGTAAAT ACACAGTGGT GATGGACGAT 
GAGAAAGGGG GCCTTGACAC CCTTGCTGTG AGGAGCGGGG ACATGGTAGA GGTGGTGGAA 
GAGGGCGCAG AGGGGCTCTG GTATGTCCGG GACCTGACCA GCAGCAAGGA GGGATGGGTG 
CCAGCCAGCA GCCTTTCCAC CCTCCTGGGC AAATCCAGTT CAGCCCAGTG TCTGAGCAGC 
TCAGACTCCA GCCCAGGGTC AGCCGTGCTG AGCAACTCCT CCAGCTGCAG CGAGGGCTGC 
CCCACTCCCT TCTCCGACCT CCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846629.1
CDS	165..3794
Translation	MSDCWRFILCKDRGRDSPSPQEHNGVPKEEADHQVDVSDGIKLVPDKAEAMVTAPDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_008848407.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848407.2

ORF Insert Sequence:

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG ACGATGCGGT CCCTAGTTCT 
ACCTCTGAAA GCTCTGCGCT TTCCAGAAAG CGCTTCACGC TGCAGGGCTT GGCTAACCTC 
AAAGGTCAGA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAAGCC 
CCTGAGGAGG ACGGAGGTTG GTCCAGTGCT GAGGAGCTTG TCAATTCATC TGATGCAGAG 
GAAGATGGAG TGGGCCCCAA GAAGCTGGTT CCTGGTAAAT ACACAGTGGT GATGGACGAT 
GAGAAAGGGG GCCTTGACAC CCTTGCTGTG AGGAGCGGGG ACATGGTAGA GGTGGTGGAA 
GAGGGCGCAG AGGGGCTCTG GTATGTCCGG GACCTGACCA GCAGCAAGGA GGGATGGGTG 
CCAGCCAGCA GCCTTTCCAC CCTCCTGGGC AAATCCAGTT CAGCCCAGTG TCTGAGCAGC 
TCAGACTCCA GCCCAGGGTC AGCCGTGCTG AGCAACTCCT CCAGCTGCAG CGAGGGCTGC 
CCCACTCCCT TCTCCGACCT CCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_029553699.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029409559.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONa31072
Clone ID Related Accession (Same CDS sequence)	XM_008848407.1 , XM_008848407.2 , XM_029553699.1
Accession Version	XM_029553699.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG ACGATGCGGT CCCTAGTTCT 
ACCTCTGAAA GCTCTGCGCT TTCCAGAAAG CGCTTCACGC TGCAGGGCTT GGCTAACCTC 
AAAGGTCAGA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAAGCC 
CCTGAGGAGG ACGGAGGTTG GTCCAGTGCT GAGGAGCTTG TCAATTCATC TGATGCAGAG 
GAAGATGGAG TGGGCCCCAA GAAGCTGGTT CCTGGTAAAT ACACAGTGGT GATGGACGAT 
GAGAAAGGGG GCCTTGACAC CCTTGCTGTG AGGAGCGGGG ACATGGTAGA GGTGGTGGAA 
GAGGGCGCAG AGGGGCTCTG GTATGTCCGG GACCTGACCA GCAGCAAGGA GGGATGGGTG 
CCAGCCAGCA GCCTTTCCAC CCTCCTGGGC AAATCCAGTT CAGCCCAGTG TCTGAGCAGC 
TCAGACTCCA GCCCAGGGTC AGCCGTGCTG AGCAACTCCT CCAGCTGCAG CGAGGGCTGC 
CCCACTCCCT TCTCCGACCT CCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029409559.1
CDS	51..3680
Translation	MSDCWRFILCKDRGRDSPSPQEHNGVPKEEADHQVDVSDGIKLVPDKAEAMVTAPDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_029553699.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029553699.1

ORF Insert Sequence:

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG ACGATGCGGT CCCTAGTTCT 
ACCTCTGAAA GCTCTGCGCT TTCCAGAAAG CGCTTCACGC TGCAGGGCTT GGCTAACCTC 
AAAGGTCAGA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAAGCC 
CCTGAGGAGG ACGGAGGTTG GTCCAGTGCT GAGGAGCTTG TCAATTCATC TGATGCAGAG 
GAAGATGGAG TGGGCCCCAA GAAGCTGGTT CCTGGTAAAT ACACAGTGGT GATGGACGAT 
GAGAAAGGGG GCCTTGACAC CCTTGCTGTG AGGAGCGGGG ACATGGTAGA GGTGGTGGAA 
GAGGGCGCAG AGGGGCTCTG GTATGTCCGG GACCTGACCA GCAGCAAGGA GGGATGGGTG 
CCAGCCAGCA GCCTTTCCAC CCTCCTGGGC AAATCCAGTT CAGCCCAGTG TCTGAGCAGC 
TCAGACTCCA GCCCAGGGTC AGCCGTGCTG AGCAACTCCT CCAGCTGCAG CGAGGGCTGC 
CCCACTCCCT TCTCCGACCT CCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848408.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846630.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONa31073
Clone ID Related Accession (Same CDS sequence)	XM_008848408.1 , XM_008848408.2
Accession Version	XM_008848408.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG ACGATGCGGT CCCTAGTTCT 
ACCTCTGAAA GCTCTGCGCT TTCCAGAAAG CGCTTCACGC TGCAGGGCTT GGCTAACCTC 
AAAGGTCAGA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAAGCC 
CCTGAGGAGG ACGGAGGTTG GTCCAGTGCT GAGGAGCTTG TCAATTCATC TGATGCAGAG 
GAAGATGGAG TGGGCCCCAA GAAGCTGGTT CCTGGTAAAT ACACAGTGGT GATGGACGAT 
GAGAAAGGGG GCCTTGACAC CCTTGCTGTG AGGAGCGGGG ACATGGTAGA GGTGGTGGAA 
GAGGGCGCAG AGGGGCTCTG GTATGTCCGG GACCTGACCA GCAGCAAGGA GGGATGGGTG 
CCAGCCAGCA GCCTTTCCAC CCTCCTGGGC AAATCCAGTT CAGCCCAGTG TCTGAGCAGC 
TCAGGTGTGC AGAGGAAAGA GCCAGAGCCG AAGCCTGTCT CCACTGGTTT CCCGCAGACT 
CCAGCCCAGG GTCAGCCGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846630.1
CDS	29..3652
Translation	MSDCWRFILCKDRGRDSPSPQEHNGVPKEEADHQVDVSDGIKLVPDKAEAMVTAPDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGVQRKEPEPKPVSTGFPQTPAQGQPC

Target ORF information:

RefSeq Version	XM_008848408.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848408.1

ORF Insert Sequence:

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG ACGATGCGGT CCCTAGTTCT 
ACCTCTGAAA GCTCTGCGCT TTCCAGAAAG CGCTTCACGC TGCAGGGCTT GGCTAACCTC 
AAAGGTCAGA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAAGCC 
CCTGAGGAGG ACGGAGGTTG GTCCAGTGCT GAGGAGCTTG TCAATTCATC TGATGCAGAG 
GAAGATGGAG TGGGCCCCAA GAAGCTGGTT CCTGGTAAAT ACACAGTGGT GATGGACGAT 
GAGAAAGGGG GCCTTGACAC CCTTGCTGTG AGGAGCGGGG ACATGGTAGA GGTGGTGGAA 
GAGGGCGCAG AGGGGCTCTG GTATGTCCGG GACCTGACCA GCAGCAAGGA GGGATGGGTG 
CCAGCCAGCA GCCTTTCCAC CCTCCTGGGC AAATCCAGTT CAGCCCAGTG TCTGAGCAGC 
TCAGGTGTGC AGAGGAAAGA GCCAGAGCCG AAGCCTGTCT CCACTGGTTT CCCGCAGACT 
CCAGCCCAGG GTCAGCCGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848408.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846630.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ONa31073
Clone ID Related Accession (Same CDS sequence)	XM_008848408.1 , XM_008848408.2
Accession Version	XM_008848408.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008848408.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG ACGATGCGGT CCCTAGTTCT 
ACCTCTGAAA GCTCTGCGCT TTCCAGAAAG CGCTTCACGC TGCAGGGCTT GGCTAACCTC 
AAAGGTCAGA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAAGCC 
CCTGAGGAGG ACGGAGGTTG GTCCAGTGCT GAGGAGCTTG TCAATTCATC TGATGCAGAG 
GAAGATGGAG TGGGCCCCAA GAAGCTGGTT CCTGGTAAAT ACACAGTGGT GATGGACGAT 
GAGAAAGGGG GCCTTGACAC CCTTGCTGTG AGGAGCGGGG ACATGGTAGA GGTGGTGGAA 
GAGGGCGCAG AGGGGCTCTG GTATGTCCGG GACCTGACCA GCAGCAAGGA GGGATGGGTG 
CCAGCCAGCA GCCTTTCCAC CCTCCTGGGC AAATCCAGTT CAGCCCAGTG TCTGAGCAGC 
TCAGGTGTGC AGAGGAAAGA GCCAGAGCCG AAGCCTGTCT CCACTGGTTT CCCGCAGACT 
CCAGCCCAGG GTCAGCCGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846630.1
CDS	166..3789
Translation	MSDCWRFILCKDRGRDSPSPQEHNGVPKEEADHQVDVSDGIKLVPDKAEAMVTAPDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGVQRKEPEPKPVSTGFPQTPAQGQPC

Target ORF information:

RefSeq Version	XM_008848408.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848408.2

ORF Insert Sequence:

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG ACGATGCGGT CCCTAGTTCT 
ACCTCTGAAA GCTCTGCGCT TTCCAGAAAG CGCTTCACGC TGCAGGGCTT GGCTAACCTC 
AAAGGTCAGA AAGCCTCTCC GACCAGTCCT GACAAAAAAG CTAAGCGGCA CGAAGTAAAG 
AGCGACCCGA CTCCCTTTGG TTTGCGAGGC TGGAGCAAAA CATCCCACTC CCTGGAAGCC 
CCTGAGGAGG ACGGAGGTTG GTCCAGTGCT GAGGAGCTTG TCAATTCATC TGATGCAGAG 
GAAGATGGAG TGGGCCCCAA GAAGCTGGTT CCTGGTAAAT ACACAGTGGT GATGGACGAT 
GAGAAAGGGG GCCTTGACAC CCTTGCTGTG AGGAGCGGGG ACATGGTAGA GGTGGTGGAA 
GAGGGCGCAG AGGGGCTCTG GTATGTCCGG GACCTGACCA GCAGCAAGGA GGGATGGGTG 
CCAGCCAGCA GCCTTTCCAC CCTCCTGGGC AAATCCAGTT CAGCCCAGTG TCTGAGCAGC 
TCAGGTGTGC AGAGGAAAGA GCCAGAGCCG AAGCCTGTCT CCACTGGTTT CCCGCAGACT 
CCAGCCCAGG GTCAGCCGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848409.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846631.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X3, mRNA.

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CloneID	ONa31074
Clone ID Related Accession (Same CDS sequence)	XM_008848409.2 , XM_008848409.3
Accession Version	XM_008848409.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008848409.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGA AGGGTACATC TTGGGGGGCC ATGCGGCGTT TCTGGTCACT GCCAAGAAAG 
CGCCGGGGAG CTTCAGGGCG CCACAAGCCA GATGAAATCA TGCACCAGGA CATCGTCCCA 
CTCTGTGCTG CGGACATCCA GGAGCAGCTG AAGAAGCGCT TTGCTTACCT GTCTGGTGGG 
CGGGGGCAGG ACGGAAGCCC AGTTATCACC TTTCCAGACT ACCCAGCCTT CAGCGAGATC 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TATCTCACCA GCATCCCCAG TTTGCAAGAT 
GCTGGCATTG GATTCATTCT GGTCATAGAC CGAAGGCAGG ACAAATGGAC CTCCGTGAAG 
GCGTCGGTCC TGCGAATAGC CGCATCTTTC CCAGCAAACC TGCAGCTCGT CCTTGTGCTT 
CGCCCAACGG GCTTTTTCCA GAGGACTCTC TCTGACCTCG CCTTCAAGTT CAACAGAGAT 
GACTTTAAGA TGAAGGTGCC GGTCATGATG CTGAGCTCAG TGCCAGAACT ACACGGCTAC 
ATTGATAAGT CACAGCTGAC CGAGGACCTC GGGGGGACCC TGGACTACTG CCATTCCAGG 
TGGCTGTGCC ACCGCACGGC GATCGAAAGC TTCGCCCTGA TGGTGAAGCA GACAGCTCAG 
ATGCTGCAGG CCTTCGGGAC CGAGCTGGCC GAAACAGAAC TGCCCAACGA TGTCCAATCA 
ACAAGCTCGG TGCTGAGCAC ACACACGGAG AAGAAGGACA AAGCAAAGGA GGATCTGCAG 
CTGGCACGGA AAGAGGGGAA CAGCATCCTT GAGAGCCTCA GGGAGCCACT GGCTGAAAGC 
ACTGCCCACA GTGTAAACCA AGACCAGCTG GACAATCAGG CCACAGTGCA GAGGCTCCTG 
GCTCAGCTGA ATGAAACGGA GGCCGCCTTT GACGAGTTCT GGGCAAAGCA TCAGCAGAAG 
CTGGAACAGT GCCTGCAGCT GCGGCATTTT GAGCAGGGTT TCCGGGAGGT CAAAACTACC 
TTGGATACCA TGTCCCACAA GATAGCCACC TTCACTGACG TTGGTAACAG CCTGGCACAT 
GTGCAACACC TGCTGAAGGA CCTGGCCAGC TTCGAAGAGA AGTCTAGCGT GGCTGTAGAC 
AGGGCCCGAG CCCTGTCACT GGAGGGTCAG CAGCTGATTG AGAACAAGCA CTATGCTGTG 
GACTCCATCC ACCCTAAGTG TGAGGAGCTC CAGCACCTCT GCGATCACTT CACCGCTGAG 
ATCAGCAGGA GGAGAGGCTT GCTCAGCAAG TCCCTGGAGC TACACAGCCT CTTGGAGACG 
TCCATGAAGT GGTGCGATGA GGGCATCTTC CTGCTGGCCT CGCAACCTGT GGACAAGTGT 
CAGTCCCAGG ATGGTGCTGA GGCAGCCCTC CAGGAGATCG AGAAATTTTT GGAGACTGGT 
GCAGAAAATA AGATTCAAGA ACTCAATAAA ATTTACAAGG AATATGAGTG CATCCTCAAC 
CAGGACCTAC TGGAACACGT GCAAAAGGTC TTTCAGAAGC AAGAAAGTAT GCAAGAGATG 
TTCCACCGCA GGCAGGCCAG CCTGAAGAAG CTGGCGGCCA AGCAGACACG GCCTGTGCAG 
CCCGTGGCCC CACGGCCAGA GGCACTCACC AAATCACCCT GCCCCTCCCC AGGCTCCTGG 
AGAGGATCTG AGAACTCTAG CTCTGAGGGC AGTGCACTCC GTAGAGGGCC CTACAGGAGA 
GCCAAGAGTG AAATGAGTGA GACCCGGCAG GGCCGTGCCA GCTCCACAGG GGACGAGGAG 
GAGAGCCTAG CCATCCTGCG CAGGCATGTG ATGAATGAGC TCCTAGACAC GGAACGGGCC 
TACGTGGAGG AGCTGCTCTG TGTACTAGAG GGCTATGCTG CAGAGATGGA CAACCCCCTG 
ATGGCACACC TCATTTCAGC AGGCCTGCAA AACAAGAAGA ACATTCTGTT TGGAAACATG 
GAGGAGATTT ATCACTTTCA TAACAGGATA TTCCTGCGGG AGCTAGAGAG CTGCATAGAC 
TGCCCGGAAC TGGTGGGGAG GTGCTTTCTG GAGAGGATGG AGGAGTTCCA GATCTACGAG 
AAGTACTGTC AGAACAAGCC ACGATCTGAG AGCCTGTGGA GACAGTGCTC TGACTGTCCC 
TTCTTCCAGG AGTGCCAGAA GAAGTTGGAC CACAAACTGA GCCTTGATTC CTACCTGCTG 
AAGCCAGTGC AGAGAATAAC TAAGTACCAG TTGCTGCTTA AGGAAATGTT GAAATACAGC 
AAGAACTGTG AGGGGGCAGA GGACCTACAG GAGGCACTGA GCTCCATCCT GGGCATCCTC 
AAGGCAGTGA ATGACTCCAT GCACCTCATC GCCATCACTG GCTACGACGG TAACCTGGGT 
GATCTGGGGA AGCTGCTGAT GCAAGGTTCC TTCAGTGTAT GGACAGACCA CAAGAAGGGC 
CACACCAAGG TCAAGGAGCT GGCCAGATTC AAGCCCATGC AGCGGCACCT TTTCCTGCAT 
GAGAAGGCTG TGCTGTTCTG CAAGAAGAGG GAGGAGAATG GGGAGGGGTA TGAGAAGGCC 
CCTTCCTACA GCTACAAGCA GTCATTGAAT ATGACTGCTG TTGGCATCAC AGAGAATGTG 
AAGGGTGATG CCAAGAAGTT CGAGATCTGG TACAACGCAA GAGAGGAGGT TTACATCATC 
CAGGCACCAA CCCCTGAGAT TAAAGCAGCA TGGGTGAGTG AGATTCGGAA GGTGCTGACC 
AGCCAGCTGC AGGCCTGCCG GGAAGCCAGC CAGCACAGGG CCCTGGAGCA GTCCCACAGC 
CTGCCTTTGC CCACACCATC CAACACCAGC CCCTCAAAAG GGAACACAAG GAATGTCAAG 
AAGTCAGAAG AAAGAAAAAC GGACCCCCTG AGCCTGGAAG GATATGTGAG CTCATCGTTG 
CCGAAGCCCC CTGAGAAAGG CAAAGACGAT GCGGTCCCTA GTTCTACCTC TGAAAGCTCT 
GCGCTTTCCA GAAAGCGCTT CACGCTGCAG GGCTTGGCTA ACCTCAAAGG TCAGAAAGCC 
TCTCCGACCA GTCCTGACAA AAAAGCTAAG CGGCACGAAG TAAAGAGCGA CCCGACTCCC 
TTTGGTTTGC GAGGCTGGAG CAAAACATCC CACTCCCTGG AAGCCCCTGA GGAGGACGGA 
GGTTGGTCCA GTGCTGAGGA GCTTGTCAAT TCATCTGATG CAGAGGAAGA TGGAGTGGGC 
CCCAAGAAGC TGGTTCCTGG TAAATACACA GTGGTGATGG ACGATGAGAA AGGGGGCCTT 
GACACCCTTG CTGTGAGGAG CGGGGACATG GTAGAGGTGG TGGAAGAGGG CGCAGAGGGG 
CTCTGGTATG TCCGGGACCT GACCAGCAGC AAGGAGGGAT GGGTGCCAGC CAGCAGCCTT 
TCCACCCTCC TGGGCAAATC CAGTTCAGCC CAGTGTCTGA GCAGCTCAGA CTCCAGCCCA 
GGGTCAGCCG TGCTGAGCAA CTCCTCCAGC TGCAGCGAGG GCTGCCCCAC TCCCTTCTCC 
GACCTCCAGG GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846631.1
CDS	380..3934
Translation	MAEKGTSWGAMRRFWSLPRKRRGASGRHKPDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_008848409.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848409.2

ORF Insert Sequence:

ATGGCAGAGA AGGGTACATC TTGGGGGGCC ATGCGGCGTT TCTGGTCACT GCCAAGAAAG 
CGCCGGGGAG CTTCAGGGCG CCACAAGCCA GATGAAATCA TGCACCAGGA CATCGTCCCA 
CTCTGTGCTG CGGACATCCA GGAGCAGCTG AAGAAGCGCT TTGCTTACCT GTCTGGTGGG 
CGGGGGCAGG ACGGAAGCCC AGTTATCACC TTTCCAGACT ACCCAGCCTT CAGCGAGATC 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TATCTCACCA GCATCCCCAG TTTGCAAGAT 
GCTGGCATTG GATTCATTCT GGTCATAGAC CGAAGGCAGG ACAAATGGAC CTCCGTGAAG 
GCGTCGGTCC TGCGAATAGC CGCATCTTTC CCAGCAAACC TGCAGCTCGT CCTTGTGCTT 
CGCCCAACGG GCTTTTTCCA GAGGACTCTC TCTGACCTCG CCTTCAAGTT CAACAGAGAT 
GACTTTAAGA TGAAGGTGCC GGTCATGATG CTGAGCTCAG TGCCAGAACT ACACGGCTAC 
ATTGATAAGT CACAGCTGAC CGAGGACCTC GGGGGGACCC TGGACTACTG CCATTCCAGG 
TGGCTGTGCC ACCGCACGGC GATCGAAAGC TTCGCCCTGA TGGTGAAGCA GACAGCTCAG 
ATGCTGCAGG CCTTCGGGAC CGAGCTGGCC GAAACAGAAC TGCCCAACGA TGTCCAATCA 
ACAAGCTCGG TGCTGAGCAC ACACACGGAG AAGAAGGACA AAGCAAAGGA GGATCTGCAG 
CTGGCACGGA AAGAGGGGAA CAGCATCCTT GAGAGCCTCA GGGAGCCACT GGCTGAAAGC 
ACTGCCCACA GTGTAAACCA AGACCAGCTG GACAATCAGG CCACAGTGCA GAGGCTCCTG 
GCTCAGCTGA ATGAAACGGA GGCCGCCTTT GACGAGTTCT GGGCAAAGCA TCAGCAGAAG 
CTGGAACAGT GCCTGCAGCT GCGGCATTTT GAGCAGGGTT TCCGGGAGGT CAAAACTACC 
TTGGATACCA TGTCCCACAA GATAGCCACC TTCACTGACG TTGGTAACAG CCTGGCACAT 
GTGCAACACC TGCTGAAGGA CCTGGCCAGC TTCGAAGAGA AGTCTAGCGT GGCTGTAGAC 
AGGGCCCGAG CCCTGTCACT GGAGGGTCAG CAGCTGATTG AGAACAAGCA CTATGCTGTG 
GACTCCATCC ACCCTAAGTG TGAGGAGCTC CAGCACCTCT GCGATCACTT CACCGCTGAG 
ATCAGCAGGA GGAGAGGCTT GCTCAGCAAG TCCCTGGAGC TACACAGCCT CTTGGAGACG 
TCCATGAAGT GGTGCGATGA GGGCATCTTC CTGCTGGCCT CGCAACCTGT GGACAAGTGT 
CAGTCCCAGG ATGGTGCTGA GGCAGCCCTC CAGGAGATCG AGAAATTTTT GGAGACTGGT 
GCAGAAAATA AGATTCAAGA ACTCAATAAA ATTTACAAGG AATATGAGTG CATCCTCAAC 
CAGGACCTAC TGGAACACGT GCAAAAGGTC TTTCAGAAGC AAGAAAGTAT GCAAGAGATG 
TTCCACCGCA GGCAGGCCAG CCTGAAGAAG CTGGCGGCCA AGCAGACACG GCCTGTGCAG 
CCCGTGGCCC CACGGCCAGA GGCACTCACC AAATCACCCT GCCCCTCCCC AGGCTCCTGG 
AGAGGATCTG AGAACTCTAG CTCTGAGGGC AGTGCACTCC GTAGAGGGCC CTACAGGAGA 
GCCAAGAGTG AAATGAGTGA GACCCGGCAG GGCCGTGCCA GCTCCACAGG GGACGAGGAG 
GAGAGCCTAG CCATCCTGCG CAGGCATGTG ATGAATGAGC TCCTAGACAC GGAACGGGCC 
TACGTGGAGG AGCTGCTCTG TGTACTAGAG GGCTATGCTG CAGAGATGGA CAACCCCCTG 
ATGGCACACC TCATTTCAGC AGGCCTGCAA AACAAGAAGA ACATTCTGTT TGGAAACATG 
GAGGAGATTT ATCACTTTCA TAACAGGATA TTCCTGCGGG AGCTAGAGAG CTGCATAGAC 
TGCCCGGAAC TGGTGGGGAG GTGCTTTCTG GAGAGGATGG AGGAGTTCCA GATCTACGAG 
AAGTACTGTC AGAACAAGCC ACGATCTGAG AGCCTGTGGA GACAGTGCTC TGACTGTCCC 
TTCTTCCAGG AGTGCCAGAA GAAGTTGGAC CACAAACTGA GCCTTGATTC CTACCTGCTG 
AAGCCAGTGC AGAGAATAAC TAAGTACCAG TTGCTGCTTA AGGAAATGTT GAAATACAGC 
AAGAACTGTG AGGGGGCAGA GGACCTACAG GAGGCACTGA GCTCCATCCT GGGCATCCTC 
AAGGCAGTGA ATGACTCCAT GCACCTCATC GCCATCACTG GCTACGACGG TAACCTGGGT 
GATCTGGGGA AGCTGCTGAT GCAAGGTTCC TTCAGTGTAT GGACAGACCA CAAGAAGGGC 
CACACCAAGG TCAAGGAGCT GGCCAGATTC AAGCCCATGC AGCGGCACCT TTTCCTGCAT 
GAGAAGGCTG TGCTGTTCTG CAAGAAGAGG GAGGAGAATG GGGAGGGGTA TGAGAAGGCC 
CCTTCCTACA GCTACAAGCA GTCATTGAAT ATGACTGCTG TTGGCATCAC AGAGAATGTG 
AAGGGTGATG CCAAGAAGTT CGAGATCTGG TACAACGCAA GAGAGGAGGT TTACATCATC 
CAGGCACCAA CCCCTGAGAT TAAAGCAGCA TGGGTGAGTG AGATTCGGAA GGTGCTGACC 
AGCCAGCTGC AGGCCTGCCG GGAAGCCAGC CAGCACAGGG CCCTGGAGCA GTCCCACAGC 
CTGCCTTTGC CCACACCATC CAACACCAGC CCCTCAAAAG GGAACACAAG GAATGTCAAG 
AAGTCAGAAG AAAGAAAAAC GGACCCCCTG AGCCTGGAAG GATATGTGAG CTCATCGTTG 
CCGAAGCCCC CTGAGAAAGG CAAAGACGAT GCGGTCCCTA GTTCTACCTC TGAAAGCTCT 
GCGCTTTCCA GAAAGCGCTT CACGCTGCAG GGCTTGGCTA ACCTCAAAGG TCAGAAAGCC 
TCTCCGACCA GTCCTGACAA AAAAGCTAAG CGGCACGAAG TAAAGAGCGA CCCGACTCCC 
TTTGGTTTGC GAGGCTGGAG CAAAACATCC CACTCCCTGG AAGCCCCTGA GGAGGACGGA 
GGTTGGTCCA GTGCTGAGGA GCTTGTCAAT TCATCTGATG CAGAGGAAGA TGGAGTGGGC 
CCCAAGAAGC TGGTTCCTGG TAAATACACA GTGGTGATGG ACGATGAGAA AGGGGGCCTT 
GACACCCTTG CTGTGAGGAG CGGGGACATG GTAGAGGTGG TGGAAGAGGG CGCAGAGGGG 
CTCTGGTATG TCCGGGACCT GACCAGCAGC AAGGAGGGAT GGGTGCCAGC CAGCAGCCTT 
TCCACCCTCC TGGGCAAATC CAGTTCAGCC CAGTGTCTGA GCAGCTCAGA CTCCAGCCCA 
GGGTCAGCCG TGCTGAGCAA CTCCTCCAGC TGCAGCGAGG GCTGCCCCAC TCCCTTCTCC 
GACCTCCAGG GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848409.3 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846631.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ONa31074
Clone ID Related Accession (Same CDS sequence)	XM_008848409.2 , XM_008848409.3
Accession Version	XM_008848409.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008848409.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGA AGGGTACATC TTGGGGGGCC ATGCGGCGTT TCTGGTCACT GCCAAGAAAG 
CGCCGGGGAG CTTCAGGGCG CCACAAGCCA GATGAAATCA TGCACCAGGA CATCGTCCCA 
CTCTGTGCTG CGGACATCCA GGAGCAGCTG AAGAAGCGCT TTGCTTACCT GTCTGGTGGG 
CGGGGGCAGG ACGGAAGCCC AGTTATCACC TTTCCAGACT ACCCAGCCTT CAGCGAGATC 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TATCTCACCA GCATCCCCAG TTTGCAAGAT 
GCTGGCATTG GATTCATTCT GGTCATAGAC CGAAGGCAGG ACAAATGGAC CTCCGTGAAG 
GCGTCGGTCC TGCGAATAGC CGCATCTTTC CCAGCAAACC TGCAGCTCGT CCTTGTGCTT 
CGCCCAACGG GCTTTTTCCA GAGGACTCTC TCTGACCTCG CCTTCAAGTT CAACAGAGAT 
GACTTTAAGA TGAAGGTGCC GGTCATGATG CTGAGCTCAG TGCCAGAACT ACACGGCTAC 
ATTGATAAGT CACAGCTGAC CGAGGACCTC GGGGGGACCC TGGACTACTG CCATTCCAGG 
TGGCTGTGCC ACCGCACGGC GATCGAAAGC TTCGCCCTGA TGGTGAAGCA GACAGCTCAG 
ATGCTGCAGG CCTTCGGGAC CGAGCTGGCC GAAACAGAAC TGCCCAACGA TGTCCAATCA 
ACAAGCTCGG TGCTGAGCAC ACACACGGAG AAGAAGGACA AAGCAAAGGA GGATCTGCAG 
CTGGCACGGA AAGAGGGGAA CAGCATCCTT GAGAGCCTCA GGGAGCCACT GGCTGAAAGC 
ACTGCCCACA GTGTAAACCA AGACCAGCTG GACAATCAGG CCACAGTGCA GAGGCTCCTG 
GCTCAGCTGA ATGAAACGGA GGCCGCCTTT GACGAGTTCT GGGCAAAGCA TCAGCAGAAG 
CTGGAACAGT GCCTGCAGCT GCGGCATTTT GAGCAGGGTT TCCGGGAGGT CAAAACTACC 
TTGGATACCA TGTCCCACAA GATAGCCACC TTCACTGACG TTGGTAACAG CCTGGCACAT 
GTGCAACACC TGCTGAAGGA CCTGGCCAGC TTCGAAGAGA AGTCTAGCGT GGCTGTAGAC 
AGGGCCCGAG CCCTGTCACT GGAGGGTCAG CAGCTGATTG AGAACAAGCA CTATGCTGTG 
GACTCCATCC ACCCTAAGTG TGAGGAGCTC CAGCACCTCT GCGATCACTT CACCGCTGAG 
ATCAGCAGGA GGAGAGGCTT GCTCAGCAAG TCCCTGGAGC TACACAGCCT CTTGGAGACG 
TCCATGAAGT GGTGCGATGA GGGCATCTTC CTGCTGGCCT CGCAACCTGT GGACAAGTGT 
CAGTCCCAGG ATGGTGCTGA GGCAGCCCTC CAGGAGATCG AGAAATTTTT GGAGACTGGT 
GCAGAAAATA AGATTCAAGA ACTCAATAAA ATTTACAAGG AATATGAGTG CATCCTCAAC 
CAGGACCTAC TGGAACACGT GCAAAAGGTC TTTCAGAAGC AAGAAAGTAT GCAAGAGATG 
TTCCACCGCA GGCAGGCCAG CCTGAAGAAG CTGGCGGCCA AGCAGACACG GCCTGTGCAG 
CCCGTGGCCC CACGGCCAGA GGCACTCACC AAATCACCCT GCCCCTCCCC AGGCTCCTGG 
AGAGGATCTG AGAACTCTAG CTCTGAGGGC AGTGCACTCC GTAGAGGGCC CTACAGGAGA 
GCCAAGAGTG AAATGAGTGA GACCCGGCAG GGCCGTGCCA GCTCCACAGG GGACGAGGAG 
GAGAGCCTAG CCATCCTGCG CAGGCATGTG ATGAATGAGC TCCTAGACAC GGAACGGGCC 
TACGTGGAGG AGCTGCTCTG TGTACTAGAG GGCTATGCTG CAGAGATGGA CAACCCCCTG 
ATGGCACACC TCATTTCAGC AGGCCTGCAA AACAAGAAGA ACATTCTGTT TGGAAACATG 
GAGGAGATTT ATCACTTTCA TAACAGGATA TTCCTGCGGG AGCTAGAGAG CTGCATAGAC 
TGCCCGGAAC TGGTGGGGAG GTGCTTTCTG GAGAGGATGG AGGAGTTCCA GATCTACGAG 
AAGTACTGTC AGAACAAGCC ACGATCTGAG AGCCTGTGGA GACAGTGCTC TGACTGTCCC 
TTCTTCCAGG AGTGCCAGAA GAAGTTGGAC CACAAACTGA GCCTTGATTC CTACCTGCTG 
AAGCCAGTGC AGAGAATAAC TAAGTACCAG TTGCTGCTTA AGGAAATGTT GAAATACAGC 
AAGAACTGTG AGGGGGCAGA GGACCTACAG GAGGCACTGA GCTCCATCCT GGGCATCCTC 
AAGGCAGTGA ATGACTCCAT GCACCTCATC GCCATCACTG GCTACGACGG TAACCTGGGT 
GATCTGGGGA AGCTGCTGAT GCAAGGTTCC TTCAGTGTAT GGACAGACCA CAAGAAGGGC 
CACACCAAGG TCAAGGAGCT GGCCAGATTC AAGCCCATGC AGCGGCACCT TTTCCTGCAT 
GAGAAGGCTG TGCTGTTCTG CAAGAAGAGG GAGGAGAATG GGGAGGGGTA TGAGAAGGCC 
CCTTCCTACA GCTACAAGCA GTCATTGAAT ATGACTGCTG TTGGCATCAC AGAGAATGTG 
AAGGGTGATG CCAAGAAGTT CGAGATCTGG TACAACGCAA GAGAGGAGGT TTACATCATC 
CAGGCACCAA CCCCTGAGAT TAAAGCAGCA TGGGTGAGTG AGATTCGGAA GGTGCTGACC 
AGCCAGCTGC AGGCCTGCCG GGAAGCCAGC CAGCACAGGG CCCTGGAGCA GTCCCACAGC 
CTGCCTTTGC CCACACCATC CAACACCAGC CCCTCAAAAG GGAACACAAG GAATGTCAAG 
AAGTCAGAAG AAAGAAAAAC GGACCCCCTG AGCCTGGAAG GATATGTGAG CTCATCGTTG 
CCGAAGCCCC CTGAGAAAGG CAAAGACGAT GCGGTCCCTA GTTCTACCTC TGAAAGCTCT 
GCGCTTTCCA GAAAGCGCTT CACGCTGCAG GGCTTGGCTA ACCTCAAAGG TCAGAAAGCC 
TCTCCGACCA GTCCTGACAA AAAAGCTAAG CGGCACGAAG TAAAGAGCGA CCCGACTCCC 
TTTGGTTTGC GAGGCTGGAG CAAAACATCC CACTCCCTGG AAGCCCCTGA GGAGGACGGA 
GGTTGGTCCA GTGCTGAGGA GCTTGTCAAT TCATCTGATG CAGAGGAAGA TGGAGTGGGC 
CCCAAGAAGC TGGTTCCTGG TAAATACACA GTGGTGATGG ACGATGAGAA AGGGGGCCTT 
GACACCCTTG CTGTGAGGAG CGGGGACATG GTAGAGGTGG TGGAAGAGGG CGCAGAGGGG 
CTCTGGTATG TCCGGGACCT GACCAGCAGC AAGGAGGGAT GGGTGCCAGC CAGCAGCCTT 
TCCACCCTCC TGGGCAAATC CAGTTCAGCC CAGTGTCTGA GCAGCTCAGA CTCCAGCCCA 
GGGTCAGCCG TGCTGAGCAA CTCCTCCAGC TGCAGCGAGG GCTGCCCCAC TCCCTTCTCC 
GACCTCCAGG GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846631.1
CDS	382..3936
Translation	MAEKGTSWGAMRRFWSLPRKRRGASGRHKPDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_008848409.3
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848409.3

ORF Insert Sequence:

ATGGCAGAGA AGGGTACATC TTGGGGGGCC ATGCGGCGTT TCTGGTCACT GCCAAGAAAG 
CGCCGGGGAG CTTCAGGGCG CCACAAGCCA GATGAAATCA TGCACCAGGA CATCGTCCCA 
CTCTGTGCTG CGGACATCCA GGAGCAGCTG AAGAAGCGCT TTGCTTACCT GTCTGGTGGG 
CGGGGGCAGG ACGGAAGCCC AGTTATCACC TTTCCAGACT ACCCAGCCTT CAGCGAGATC 
CCGGACAAGG AGTTCCAGAA TGTCATGACC TATCTCACCA GCATCCCCAG TTTGCAAGAT 
GCTGGCATTG GATTCATTCT GGTCATAGAC CGAAGGCAGG ACAAATGGAC CTCCGTGAAG 
GCGTCGGTCC TGCGAATAGC CGCATCTTTC CCAGCAAACC TGCAGCTCGT CCTTGTGCTT 
CGCCCAACGG GCTTTTTCCA GAGGACTCTC TCTGACCTCG CCTTCAAGTT CAACAGAGAT 
GACTTTAAGA TGAAGGTGCC GGTCATGATG CTGAGCTCAG TGCCAGAACT ACACGGCTAC 
ATTGATAAGT CACAGCTGAC CGAGGACCTC GGGGGGACCC TGGACTACTG CCATTCCAGG 
TGGCTGTGCC ACCGCACGGC GATCGAAAGC TTCGCCCTGA TGGTGAAGCA GACAGCTCAG 
ATGCTGCAGG CCTTCGGGAC CGAGCTGGCC GAAACAGAAC TGCCCAACGA TGTCCAATCA 
ACAAGCTCGG TGCTGAGCAC ACACACGGAG AAGAAGGACA AAGCAAAGGA GGATCTGCAG 
CTGGCACGGA AAGAGGGGAA CAGCATCCTT GAGAGCCTCA GGGAGCCACT GGCTGAAAGC 
ACTGCCCACA GTGTAAACCA AGACCAGCTG GACAATCAGG CCACAGTGCA GAGGCTCCTG 
GCTCAGCTGA ATGAAACGGA GGCCGCCTTT GACGAGTTCT GGGCAAAGCA TCAGCAGAAG 
CTGGAACAGT GCCTGCAGCT GCGGCATTTT GAGCAGGGTT TCCGGGAGGT CAAAACTACC 
TTGGATACCA TGTCCCACAA GATAGCCACC TTCACTGACG TTGGTAACAG CCTGGCACAT 
GTGCAACACC TGCTGAAGGA CCTGGCCAGC TTCGAAGAGA AGTCTAGCGT GGCTGTAGAC 
AGGGCCCGAG CCCTGTCACT GGAGGGTCAG CAGCTGATTG AGAACAAGCA CTATGCTGTG 
GACTCCATCC ACCCTAAGTG TGAGGAGCTC CAGCACCTCT GCGATCACTT CACCGCTGAG 
ATCAGCAGGA GGAGAGGCTT GCTCAGCAAG TCCCTGGAGC TACACAGCCT CTTGGAGACG 
TCCATGAAGT GGTGCGATGA GGGCATCTTC CTGCTGGCCT CGCAACCTGT GGACAAGTGT 
CAGTCCCAGG ATGGTGCTGA GGCAGCCCTC CAGGAGATCG AGAAATTTTT GGAGACTGGT 
GCAGAAAATA AGATTCAAGA ACTCAATAAA ATTTACAAGG AATATGAGTG CATCCTCAAC 
CAGGACCTAC TGGAACACGT GCAAAAGGTC TTTCAGAAGC AAGAAAGTAT GCAAGAGATG 
TTCCACCGCA GGCAGGCCAG CCTGAAGAAG CTGGCGGCCA AGCAGACACG GCCTGTGCAG 
CCCGTGGCCC CACGGCCAGA GGCACTCACC AAATCACCCT GCCCCTCCCC AGGCTCCTGG 
AGAGGATCTG AGAACTCTAG CTCTGAGGGC AGTGCACTCC GTAGAGGGCC CTACAGGAGA 
GCCAAGAGTG AAATGAGTGA GACCCGGCAG GGCCGTGCCA GCTCCACAGG GGACGAGGAG 
GAGAGCCTAG CCATCCTGCG CAGGCATGTG ATGAATGAGC TCCTAGACAC GGAACGGGCC 
TACGTGGAGG AGCTGCTCTG TGTACTAGAG GGCTATGCTG CAGAGATGGA CAACCCCCTG 
ATGGCACACC TCATTTCAGC AGGCCTGCAA AACAAGAAGA ACATTCTGTT TGGAAACATG 
GAGGAGATTT ATCACTTTCA TAACAGGATA TTCCTGCGGG AGCTAGAGAG CTGCATAGAC 
TGCCCGGAAC TGGTGGGGAG GTGCTTTCTG GAGAGGATGG AGGAGTTCCA GATCTACGAG 
AAGTACTGTC AGAACAAGCC ACGATCTGAG AGCCTGTGGA GACAGTGCTC TGACTGTCCC 
TTCTTCCAGG AGTGCCAGAA GAAGTTGGAC CACAAACTGA GCCTTGATTC CTACCTGCTG 
AAGCCAGTGC AGAGAATAAC TAAGTACCAG TTGCTGCTTA AGGAAATGTT GAAATACAGC 
AAGAACTGTG AGGGGGCAGA GGACCTACAG GAGGCACTGA GCTCCATCCT GGGCATCCTC 
AAGGCAGTGA ATGACTCCAT GCACCTCATC GCCATCACTG GCTACGACGG TAACCTGGGT 
GATCTGGGGA AGCTGCTGAT GCAAGGTTCC TTCAGTGTAT GGACAGACCA CAAGAAGGGC 
CACACCAAGG TCAAGGAGCT GGCCAGATTC AAGCCCATGC AGCGGCACCT TTTCCTGCAT 
GAGAAGGCTG TGCTGTTCTG CAAGAAGAGG GAGGAGAATG GGGAGGGGTA TGAGAAGGCC 
CCTTCCTACA GCTACAAGCA GTCATTGAAT ATGACTGCTG TTGGCATCAC AGAGAATGTG 
AAGGGTGATG CCAAGAAGTT CGAGATCTGG TACAACGCAA GAGAGGAGGT TTACATCATC 
CAGGCACCAA CCCCTGAGAT TAAAGCAGCA TGGGTGAGTG AGATTCGGAA GGTGCTGACC 
AGCCAGCTGC AGGCCTGCCG GGAAGCCAGC CAGCACAGGG CCCTGGAGCA GTCCCACAGC 
CTGCCTTTGC CCACACCATC CAACACCAGC CCCTCAAAAG GGAACACAAG GAATGTCAAG 
AAGTCAGAAG AAAGAAAAAC GGACCCCCTG AGCCTGGAAG GATATGTGAG CTCATCGTTG 
CCGAAGCCCC CTGAGAAAGG CAAAGACGAT GCGGTCCCTA GTTCTACCTC TGAAAGCTCT 
GCGCTTTCCA GAAAGCGCTT CACGCTGCAG GGCTTGGCTA ACCTCAAAGG TCAGAAAGCC 
TCTCCGACCA GTCCTGACAA AAAAGCTAAG CGGCACGAAG TAAAGAGCGA CCCGACTCCC 
TTTGGTTTGC GAGGCTGGAG CAAAACATCC CACTCCCTGG AAGCCCCTGA GGAGGACGGA 
GGTTGGTCCA GTGCTGAGGA GCTTGTCAAT TCATCTGATG CAGAGGAAGA TGGAGTGGGC 
CCCAAGAAGC TGGTTCCTGG TAAATACACA GTGGTGATGG ACGATGAGAA AGGGGGCCTT 
GACACCCTTG CTGTGAGGAG CGGGGACATG GTAGAGGTGG TGGAAGAGGG CGCAGAGGGG 
CTCTGGTATG TCCGGGACCT GACCAGCAGC AAGGAGGGAT GGGTGCCAGC CAGCAGCCTT 
TCCACCCTCC TGGGCAAATC CAGTTCAGCC CAGTGTCTGA GCAGCTCAGA CTCCAGCCCA 
GGGTCAGCCG TGCTGAGCAA CTCCTCCAGC TGCAGCGAGG GCTGCCCCAC TCCCTTCTCC 
GACCTCCAGG GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848410.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846632.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ONa31075
Clone ID Related Accession (Same CDS sequence)	XM_008848410.2
Accession Version	XM_008848410.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008848410.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCTCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCACT CTGTGCTGCG 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGGCAGGAC 
GGAAGCCCAG TTATCACCTT TCCAGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATTCTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CCGTGAAGGC GTCGGTCCTG 
CGAATAGCCG CATCTTTCCC AGCAAACCTG CAGCTCGTCC TTGTGCTTCG CCCAACGGGC 
TTTTTCCAGA GGACTCTCTC TGACCTCGCC TTCAAGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATGATGCT GAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCA 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ATTCCAGGTG GCTGTGCCAC 
CGCACGGCGA TCGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCTCAGAT GCTGCAGGCC 
TTCGGGACCG AGCTGGCCGA AACAGAACTG CCCAACGATG TCCAATCAAC AAGCTCGGTG 
CTGAGCACAC ACACGGAGAA GAAGGACAAA GCAAAGGAGG ATCTGCAGCT GGCACGGAAA 
GAGGGGAACA GCATCCTTGA GAGCCTCAGG GAGCCACTGG CTGAAAGCAC TGCCCACAGT 
GTAAACCAAG ACCAGCTGGA CAATCAGGCC ACAGTGCAGA GGCTCCTGGC TCAGCTGAAT 
GAAACGGAGG CCGCCTTTGA CGAGTTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAGGGTTTC CGGGAGGTCA AAACTACCTT GGATACCATG 
TCCCACAAGA TAGCCACCTT CACTGACGTT GGTAACAGCC TGGCACATGT GCAACACCTG 
CTGAAGGACC TGGCCAGCTT CGAAGAGAAG TCTAGCGTGG CTGTAGACAG GGCCCGAGCC 
CTGTCACTGG AGGGTCAGCA GCTGATTGAG AACAAGCACT ATGCTGTGGA CTCCATCCAC 
CCTAAGTGTG AGGAGCTCCA GCACCTCTGC GATCACTTCA CCGCTGAGAT CAGCAGGAGG 
AGAGGCTTGC TCAGCAAGTC CCTGGAGCTA CACAGCCTCT TGGAGACGTC CATGAAGTGG 
TGCGATGAGG GCATCTTCCT GCTGGCCTCG CAACCTGTGG ACAAGTGTCA GTCCCAGGAT 
GGTGCTGAGG CAGCCCTCCA GGAGATCGAG AAATTTTTGG AGACTGGTGC AGAAAATAAG 
ATTCAAGAAC TCAATAAAAT TTACAAGGAA TATGAGTGCA TCCTCAACCA GGACCTACTG 
GAACACGTGC AAAAGGTCTT TCAGAAGCAA GAAAGTATGC AAGAGATGTT CCACCGCAGG 
CAGGCCAGCC TGAAGAAGCT GGCGGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCA 
CGGCCAGAGG CACTCACCAA ATCACCCTGC CCCTCCCCAG GCTCCTGGAG AGGATCTGAG 
AACTCTAGCT CTGAGGGCAG TGCACTCCGT AGAGGGCCCT ACAGGAGAGC CAAGAGTGAA 
ATGAGTGAGA CCCGGCAGGG CCGTGCCAGC TCCACAGGGG ACGAGGAGGA GAGCCTAGCC 
ATCCTGCGCA GGCATGTGAT GAATGAGCTC CTAGACACGG AACGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TACTAGAGGG CTATGCTGCA GAGATGGACA ACCCCCTGAT GGCACACCTC 
ATTTCAGCAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAGATTTAT 
CACTTTCATA ACAGGATATT CCTGCGGGAG CTAGAGAGCT GCATAGACTG CCCGGAACTG 
GTGGGGAGGT GCTTTCTGGA GAGGATGGAG GAGTTCCAGA TCTACGAGAA GTACTGTCAG 
AACAAGCCAC GATCTGAGAG CCTGTGGAGA CAGTGCTCTG ACTGTCCCTT CTTCCAGGAG 
TGCCAGAAGA AGTTGGACCA CAAACTGAGC CTTGATTCCT ACCTGCTGAA GCCAGTGCAG 
AGAATAACTA AGTACCAGTT GCTGCTTAAG GAAATGTTGA AATACAGCAA GAACTGTGAG 
GGGGCAGAGG ACCTACAGGA GGCACTGAGC TCCATCCTGG GCATCCTCAA GGCAGTGAAT 
GACTCCATGC ACCTCATCGC CATCACTGGC TACGACGGTA ACCTGGGTGA TCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGTGTATGG ACAGACCACA AGAAGGGCCA CACCAAGGTC 
AAGGAGCTGG CCAGATTCAA GCCCATGCAG CGGCACCTTT TCCTGCATGA GAAGGCTGTG 
CTGTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGGTATG AGAAGGCCCC TTCCTACAGC 
TACAAGCAGT CATTGAATAT GACTGCTGTT GGCATCACAG AGAATGTGAA GGGTGATGCC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGA GAGGAGGTTT ACATCATCCA GGCACCAACC 
CCTGAGATTA AAGCAGCATG GGTGAGTGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCCTGCCGGG AAGCCAGCCA GCACAGGGCC CTGGAGCAGT CCCACAGCCT GCCTTTGCCC 
ACACCATCCA ACACCAGCCC CTCAAAAGGG AACACAAGGA ATGTCAAGAA GTCAGAAGAA 
AGAAAAACGG ACCCCCTGAG CCTGGAAGGA TATGTGAGCT CATCGTTGCC GAAGCCCCCT 
GAGAAAGGCA AAGACGATGC GGTCCCTAGT TCTACCTCTG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC CTCAAAGGTC AGAAAGCCTC TCCGACCAGT 
CCTGACAAAA AAGCTAAGCG GCACGAAGTA AAGAGCGACC CGACTCCCTT TGGTTTGCGA 
GGCTGGAGCA AAACATCCCA CTCCCTGGAA GCCCCTGAGG AGGACGGAGG TTGGTCCAGT 
GCTGAGGAGC TTGTCAATTC ATCTGATGCA GAGGAAGATG GAGTGGGCCC CAAGAAGCTG 
GTTCCTGGTA AATACACAGT GGTGATGGAC GATGAGAAAG GGGGCCTTGA CACCCTTGCT 
GTGAGGAGCG GGGACATGGT AGAGGTGGTG GAAGAGGGCG CAGAGGGGCT CTGGTATGTC 
CGGGACCTGA CCAGCAGCAA GGAGGGATGG GTGCCAGCCA GCAGCCTTTC CACCCTCCTG 
GGCAAATCCA GTTCAGCCCA GTGTCTGAGC AGCTCAGACT CCAGCCCAGG GTCAGCCGTG 
CTGAGCAACT CCTCCAGCTG CAGCGAGGGC TGCCCCACTC CCTTCTCCGA CCTCCAGGGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846632.1
CDS	10..3552
Translation	MRFWLRNEEMALEEMVQRLNAVSKNTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_008848410.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848410.2

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCTCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCACT CTGTGCTGCG 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGGCAGGAC 
GGAAGCCCAG TTATCACCTT TCCAGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATTCTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CCGTGAAGGC GTCGGTCCTG 
CGAATAGCCG CATCTTTCCC AGCAAACCTG CAGCTCGTCC TTGTGCTTCG CCCAACGGGC 
TTTTTCCAGA GGACTCTCTC TGACCTCGCC TTCAAGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATGATGCT GAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCA 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ATTCCAGGTG GCTGTGCCAC 
CGCACGGCGA TCGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCTCAGAT GCTGCAGGCC 
TTCGGGACCG AGCTGGCCGA AACAGAACTG CCCAACGATG TCCAATCAAC AAGCTCGGTG 
CTGAGCACAC ACACGGAGAA GAAGGACAAA GCAAAGGAGG ATCTGCAGCT GGCACGGAAA 
GAGGGGAACA GCATCCTTGA GAGCCTCAGG GAGCCACTGG CTGAAAGCAC TGCCCACAGT 
GTAAACCAAG ACCAGCTGGA CAATCAGGCC ACAGTGCAGA GGCTCCTGGC TCAGCTGAAT 
GAAACGGAGG CCGCCTTTGA CGAGTTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAGGGTTTC CGGGAGGTCA AAACTACCTT GGATACCATG 
TCCCACAAGA TAGCCACCTT CACTGACGTT GGTAACAGCC TGGCACATGT GCAACACCTG 
CTGAAGGACC TGGCCAGCTT CGAAGAGAAG TCTAGCGTGG CTGTAGACAG GGCCCGAGCC 
CTGTCACTGG AGGGTCAGCA GCTGATTGAG AACAAGCACT ATGCTGTGGA CTCCATCCAC 
CCTAAGTGTG AGGAGCTCCA GCACCTCTGC GATCACTTCA CCGCTGAGAT CAGCAGGAGG 
AGAGGCTTGC TCAGCAAGTC CCTGGAGCTA CACAGCCTCT TGGAGACGTC CATGAAGTGG 
TGCGATGAGG GCATCTTCCT GCTGGCCTCG CAACCTGTGG ACAAGTGTCA GTCCCAGGAT 
GGTGCTGAGG CAGCCCTCCA GGAGATCGAG AAATTTTTGG AGACTGGTGC AGAAAATAAG 
ATTCAAGAAC TCAATAAAAT TTACAAGGAA TATGAGTGCA TCCTCAACCA GGACCTACTG 
GAACACGTGC AAAAGGTCTT TCAGAAGCAA GAAAGTATGC AAGAGATGTT CCACCGCAGG 
CAGGCCAGCC TGAAGAAGCT GGCGGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCA 
CGGCCAGAGG CACTCACCAA ATCACCCTGC CCCTCCCCAG GCTCCTGGAG AGGATCTGAG 
AACTCTAGCT CTGAGGGCAG TGCACTCCGT AGAGGGCCCT ACAGGAGAGC CAAGAGTGAA 
ATGAGTGAGA CCCGGCAGGG CCGTGCCAGC TCCACAGGGG ACGAGGAGGA GAGCCTAGCC 
ATCCTGCGCA GGCATGTGAT GAATGAGCTC CTAGACACGG AACGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TACTAGAGGG CTATGCTGCA GAGATGGACA ACCCCCTGAT GGCACACCTC 
ATTTCAGCAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAGATTTAT 
CACTTTCATA ACAGGATATT CCTGCGGGAG CTAGAGAGCT GCATAGACTG CCCGGAACTG 
GTGGGGAGGT GCTTTCTGGA GAGGATGGAG GAGTTCCAGA TCTACGAGAA GTACTGTCAG 
AACAAGCCAC GATCTGAGAG CCTGTGGAGA CAGTGCTCTG ACTGTCCCTT CTTCCAGGAG 
TGCCAGAAGA AGTTGGACCA CAAACTGAGC CTTGATTCCT ACCTGCTGAA GCCAGTGCAG 
AGAATAACTA AGTACCAGTT GCTGCTTAAG GAAATGTTGA AATACAGCAA GAACTGTGAG 
GGGGCAGAGG ACCTACAGGA GGCACTGAGC TCCATCCTGG GCATCCTCAA GGCAGTGAAT 
GACTCCATGC ACCTCATCGC CATCACTGGC TACGACGGTA ACCTGGGTGA TCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGTGTATGG ACAGACCACA AGAAGGGCCA CACCAAGGTC 
AAGGAGCTGG CCAGATTCAA GCCCATGCAG CGGCACCTTT TCCTGCATGA GAAGGCTGTG 
CTGTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGGTATG AGAAGGCCCC TTCCTACAGC 
TACAAGCAGT CATTGAATAT GACTGCTGTT GGCATCACAG AGAATGTGAA GGGTGATGCC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGA GAGGAGGTTT ACATCATCCA GGCACCAACC 
CCTGAGATTA AAGCAGCATG GGTGAGTGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCCTGCCGGG AAGCCAGCCA GCACAGGGCC CTGGAGCAGT CCCACAGCCT GCCTTTGCCC 
ACACCATCCA ACACCAGCCC CTCAAAAGGG AACACAAGGA ATGTCAAGAA GTCAGAAGAA 
AGAAAAACGG ACCCCCTGAG CCTGGAAGGA TATGTGAGCT CATCGTTGCC GAAGCCCCCT 
GAGAAAGGCA AAGACGATGC GGTCCCTAGT TCTACCTCTG AAAGCTCTGC GCTTTCCAGA 
AAGCGCTTCA CGCTGCAGGG CTTGGCTAAC CTCAAAGGTC AGAAAGCCTC TCCGACCAGT 
CCTGACAAAA AAGCTAAGCG GCACGAAGTA AAGAGCGACC CGACTCCCTT TGGTTTGCGA 
GGCTGGAGCA AAACATCCCA CTCCCTGGAA GCCCCTGAGG AGGACGGAGG TTGGTCCAGT 
GCTGAGGAGC TTGTCAATTC ATCTGATGCA GAGGAAGATG GAGTGGGCCC CAAGAAGCTG 
GTTCCTGGTA AATACACAGT GGTGATGGAC GATGAGAAAG GGGGCCTTGA CACCCTTGCT 
GTGAGGAGCG GGGACATGGT AGAGGTGGTG GAAGAGGGCG CAGAGGGGCT CTGGTATGTC 
CGGGACCTGA CCAGCAGCAA GGAGGGATGG GTGCCAGCCA GCAGCCTTTC CACCCTCCTG 
GGCAAATCCA GTTCAGCCCA GTGTCTGAGC AGCTCAGACT CCAGCCCAGG GTCAGCCGTG 
CTGAGCAACT CCTCCAGCTG CAGCGAGGGC TGCCCCACTC CCTTCTCCGA CCTCCAGGGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848413.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846635.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X6, mRNA.

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CloneID	ONa31076
Clone ID Related Accession (Same CDS sequence)	XM_008848413.2 , XM_008848413.1
Accession Version	XM_008848413.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008848413.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG CCTCTCCGAC CAGTCCTGAC 
AAAAAAGCTA AGCGGCACGA AGTAAAGAGC GACCCGACTC CCTTTGGTTT GCGAGGCTGG 
AGCAAAACAT CCCACTCCCT GGAAGCCCCT GAGGAGGACG GAGGTTGGTC CAGTGCTGAG 
GAGCTTGTCA ATTCATCTGA TGCAGAGGAA GATGGAGTGG GCCCCAAGAA GCTGGTTCCT 
GGTAAATACA CAGTGGTGAT GGACGATGAG AAAGGGGGCC TTGACACCCT TGCTGTGAGG 
AGCGGGGACA TGGTAGAGGT GGTGGAAGAG GGCGCAGAGG GGCTCTGGTA TGTCCGGGAC 
CTGACCAGCA GCAAGGAGGG ATGGGTGCCA GCCAGCAGCC TTTCCACCCT CCTGGGCAAA 
TCCAGTTCAG CCCAGTGTCT GAGCAGCTCA GACTCCAGCC CAGGGTCAGC CGTGCTGAGC 
AACTCCTCCA GCTGCAGCGA GGGCTGCCCC ACTCCCTTCT CCGACCTCCA GGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846635.1
CDS	164..3700
Translation	MSDCWRFILCKDRGRDSPSPQEHNGVPKEEADHQVDVSDGIKLVPDKAEAMVTAPDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_008848413.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848413.2

ORF Insert Sequence:

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG CCTCTCCGAC CAGTCCTGAC 
AAAAAAGCTA AGCGGCACGA AGTAAAGAGC GACCCGACTC CCTTTGGTTT GCGAGGCTGG 
AGCAAAACAT CCCACTCCCT GGAAGCCCCT GAGGAGGACG GAGGTTGGTC CAGTGCTGAG 
GAGCTTGTCA ATTCATCTGA TGCAGAGGAA GATGGAGTGG GCCCCAAGAA GCTGGTTCCT 
GGTAAATACA CAGTGGTGAT GGACGATGAG AAAGGGGGCC TTGACACCCT TGCTGTGAGG 
AGCGGGGACA TGGTAGAGGT GGTGGAAGAG GGCGCAGAGG GGCTCTGGTA TGTCCGGGAC 
CTGACCAGCA GCAAGGAGGG ATGGGTGCCA GCCAGCAGCC TTTCCACCCT CCTGGGCAAA 
TCCAGTTCAG CCCAGTGTCT GAGCAGCTCA GACTCCAGCC CAGGGTCAGC CGTGCTGAGC 
AACTCCTCCA GCTGCAGCGA GGGCTGCCCC ACTCCCTTCT CCGACCTCCA GGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848413.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846635.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X5, mRNA.

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CloneID	ONa31076
Clone ID Related Accession (Same CDS sequence)	XM_008848413.2 , XM_008848413.1
Accession Version	XM_008848413.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG CCTCTCCGAC CAGTCCTGAC 
AAAAAAGCTA AGCGGCACGA AGTAAAGAGC GACCCGACTC CCTTTGGTTT GCGAGGCTGG 
AGCAAAACAT CCCACTCCCT GGAAGCCCCT GAGGAGGACG GAGGTTGGTC CAGTGCTGAG 
GAGCTTGTCA ATTCATCTGA TGCAGAGGAA GATGGAGTGG GCCCCAAGAA GCTGGTTCCT 
GGTAAATACA CAGTGGTGAT GGACGATGAG AAAGGGGGCC TTGACACCCT TGCTGTGAGG 
AGCGGGGACA TGGTAGAGGT GGTGGAAGAG GGCGCAGAGG GGCTCTGGTA TGTCCGGGAC 
CTGACCAGCA GCAAGGAGGG ATGGGTGCCA GCCAGCAGCC TTTCCACCCT CCTGGGCAAA 
TCCAGTTCAG CCCAGTGTCT GAGCAGCTCA GACTCCAGCC CAGGGTCAGC CGTGCTGAGC 
AACTCCTCCA GCTGCAGCGA GGGCTGCCCC ACTCCCTTCT CCGACCTCCA GGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846635.1
CDS	29..3565
Translation	MSDCWRFILCKDRGRDSPSPQEHNGVPKEEADHQVDVSDGIKLVPDKAEAMVTAPDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_008848413.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848413.1

ORF Insert Sequence:

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG CCTCTCCGAC CAGTCCTGAC 
AAAAAAGCTA AGCGGCACGA AGTAAAGAGC GACCCGACTC CCTTTGGTTT GCGAGGCTGG 
AGCAAAACAT CCCACTCCCT GGAAGCCCCT GAGGAGGACG GAGGTTGGTC CAGTGCTGAG 
GAGCTTGTCA ATTCATCTGA TGCAGAGGAA GATGGAGTGG GCCCCAAGAA GCTGGTTCCT 
GGTAAATACA CAGTGGTGAT GGACGATGAG AAAGGGGGCC TTGACACCCT TGCTGTGAGG 
AGCGGGGACA TGGTAGAGGT GGTGGAAGAG GGCGCAGAGG GGCTCTGGTA TGTCCGGGAC 
CTGACCAGCA GCAAGGAGGG ATGGGTGCCA GCCAGCAGCC TTTCCACCCT CCTGGGCAAA 
TCCAGTTCAG CCCAGTGTCT GAGCAGCTCA GACTCCAGCC CAGGGTCAGC CGTGCTGAGC 
AACTCCTCCA GCTGCAGCGA GGGCTGCCCC ACTCCCTTCT CCGACCTCCA GGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848411.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846633.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ONa31077
Clone ID Related Accession (Same CDS sequence)	XM_008848411.2 , XM_008848411.3
Accession Version	XM_008848411.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008848411.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTGA GCCTCATCTC CGTGCTGTCC CAGGACGTCA CAGCCCTGTG GCTCCTGCTG 
AAGACAAGTG CAGATGAAAT CATGCACCAG GACATCGTCC CACTCTGTGC TGCGGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGGCA GGACGGAAGC 
CCAGTTATCA CCTTTCCAGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATT 
CTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCCGTGA AGGCGTCGGT CCTGCGAATA 
GCCGCATCTT TCCCAGCAAA CCTGCAGCTC GTCCTTGTGC TTCGCCCAAC GGGCTTTTTC 
CAGAGGACTC TCTCTGACCT CGCCTTCAAG TTCAACAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATGA TGCTGAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCACAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCATTCCA GGTGGCTGTG CCACCGCACG 
GCGATCGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCTC AGATGCTGCA GGCCTTCGGG 
ACCGAGCTGG CCGAAACAGA ACTGCCCAAC GATGTCCAAT CAACAAGCTC GGTGCTGAGC 
ACACACACGG AGAAGAAGGA CAAAGCAAAG GAGGATCTGC AGCTGGCACG GAAAGAGGGG 
AACAGCATCC TTGAGAGCCT CAGGGAGCCA CTGGCTGAAA GCACTGCCCA CAGTGTAAAC 
CAAGACCAGC TGGACAATCA GGCCACAGTG CAGAGGCTCC TGGCTCAGCT GAATGAAACG 
GAGGCCGCCT TTGACGAGTT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAGGG TTTCCGGGAG GTCAAAACTA CCTTGGATAC CATGTCCCAC 
AAGATAGCCA CCTTCACTGA CGTTGGTAAC AGCCTGGCAC ATGTGCAACA CCTGCTGAAG 
GACCTGGCCA GCTTCGAAGA GAAGTCTAGC GTGGCTGTAG ACAGGGCCCG AGCCCTGTCA 
CTGGAGGGTC AGCAGCTGAT TGAGAACAAG CACTATGCTG TGGACTCCAT CCACCCTAAG 
TGTGAGGAGC TCCAGCACCT CTGCGATCAC TTCACCGCTG AGATCAGCAG GAGGAGAGGC 
TTGCTCAGCA AGTCCCTGGA GCTACACAGC CTCTTGGAGA CGTCCATGAA GTGGTGCGAT 
GAGGGCATCT TCCTGCTGGC CTCGCAACCT GTGGACAAGT GTCAGTCCCA GGATGGTGCT 
GAGGCAGCCC TCCAGGAGAT CGAGAAATTT TTGGAGACTG GTGCAGAAAA TAAGATTCAA 
GAACTCAATA AAATTTACAA GGAATATGAG TGCATCCTCA ACCAGGACCT ACTGGAACAC 
GTGCAAAAGG TCTTTCAGAA GCAAGAAAGT ATGCAAGAGA TGTTCCACCG CAGGCAGGCC 
AGCCTGAAGA AGCTGGCGGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCACGGCCA 
GAGGCACTCA CCAAATCACC CTGCCCCTCC CCAGGCTCCT GGAGAGGATC TGAGAACTCT 
AGCTCTGAGG GCAGTGCACT CCGTAGAGGG CCCTACAGGA GAGCCAAGAG TGAAATGAGT 
GAGACCCGGC AGGGCCGTGC CAGCTCCACA GGGGACGAGG AGGAGAGCCT AGCCATCCTG 
CGCAGGCATG TGATGAATGA GCTCCTAGAC ACGGAACGGG CCTACGTGGA GGAGCTGCTC 
TGTGTACTAG AGGGCTATGC TGCAGAGATG GACAACCCCC TGATGGCACA CCTCATTTCA 
GCAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAGAT TTATCACTTT 
CATAACAGGA TATTCCTGCG GGAGCTAGAG AGCTGCATAG ACTGCCCGGA ACTGGTGGGG 
AGGTGCTTTC TGGAGAGGAT GGAGGAGTTC CAGATCTACG AGAAGTACTG TCAGAACAAG 
CCACGATCTG AGAGCCTGTG GAGACAGTGC TCTGACTGTC CCTTCTTCCA GGAGTGCCAG 
AAGAAGTTGG ACCACAAACT GAGCCTTGAT TCCTACCTGC TGAAGCCAGT GCAGAGAATA 
ACTAAGTACC AGTTGCTGCT TAAGGAAATG TTGAAATACA GCAAGAACTG TGAGGGGGCA 
GAGGACCTAC AGGAGGCACT GAGCTCCATC CTGGGCATCC TCAAGGCAGT GAATGACTCC 
ATGCACCTCA TCGCCATCAC TGGCTACGAC GGTAACCTGG GTGATCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGTGT ATGGACAGAC CACAAGAAGG GCCACACCAA GGTCAAGGAG 
CTGGCCAGAT TCAAGCCCAT GCAGCGGCAC CTTTTCCTGC ATGAGAAGGC TGTGCTGTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGG TATGAGAAGG CCCCTTCCTA CAGCTACAAG 
CAGTCATTGA ATATGACTGC TGTTGGCATC ACAGAGAATG TGAAGGGTGA TGCCAAGAAG 
TTCGAGATCT GGTACAACGC AAGAGAGGAG GTTTACATCA TCCAGGCACC AACCCCTGAG 
ATTAAAGCAG CATGGGTGAG TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCCTGC 
CGGGAAGCCA GCCAGCACAG GGCCCTGGAG CAGTCCCACA GCCTGCCTTT GCCCACACCA 
TCCAACACCA GCCCCTCAAA AGGGAACACA AGGAATGTCA AGAAGTCAGA AGAAAGAAAA 
ACGGACCCCC TGAGCCTGGA AGGATATGTG AGCTCATCGT TGCCGAAGCC CCCTGAGAAA 
GGCAAAGACG ATGCGGTCCC TAGTTCTACC TCTGAAAGCT CTGCGCTTTC CAGAAAGCGC 
TTCACGCTGC AGGGCTTGGC TAACCTCAAA GGTCAGAAAG CCTCTCCGAC CAGTCCTGAC 
AAAAAAGCTA AGCGGCACGA AGTAAAGAGC GACCCGACTC CCTTTGGTTT GCGAGGCTGG 
AGCAAAACAT CCCACTCCCT GGAAGCCCCT GAGGAGGACG GAGGTTGGTC CAGTGCTGAG 
GAGCTTGTCA ATTCATCTGA TGCAGAGGAA GATGGAGTGG GCCCCAAGAA GCTGGTTCCT 
GGTAAATACA CAGTGGTGAT GGACGATGAG AAAGGGGGCC TTGACACCCT TGCTGTGAGG 
AGCGGGGACA TGGTAGAGGT GGTGGAAGAG GGCGCAGAGG GGCTCTGGTA TGTCCGGGAC 
CTGACCAGCA GCAAGGAGGG ATGGGTGCCA GCCAGCAGCC TTTCCACCCT CCTGGGCAAA 
TCCAGTTCAG CCCAGTGTCT GAGCAGCTCA GACTCCAGCC CAGGGTCAGC CGTGCTGAGC 
AACTCCTCCA GCTGCAGCGA GGGCTGCCCC ACTCCCTTCT CCGACCTCCA GGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846633.1
CDS	117..3653
Translation	MTLSLISVLSQDVTALWLLLKTSADEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_008848411.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848411.2

ORF Insert Sequence:

ATGACTTTGA GCCTCATCTC CGTGCTGTCC CAGGACGTCA CAGCCCTGTG GCTCCTGCTG 
AAGACAAGTG CAGATGAAAT CATGCACCAG GACATCGTCC CACTCTGTGC TGCGGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGGCA GGACGGAAGC 
CCAGTTATCA CCTTTCCAGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATT 
CTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCCGTGA AGGCGTCGGT CCTGCGAATA 
GCCGCATCTT TCCCAGCAAA CCTGCAGCTC GTCCTTGTGC TTCGCCCAAC GGGCTTTTTC 
CAGAGGACTC TCTCTGACCT CGCCTTCAAG TTCAACAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATGA TGCTGAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCACAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCATTCCA GGTGGCTGTG CCACCGCACG 
GCGATCGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCTC AGATGCTGCA GGCCTTCGGG 
ACCGAGCTGG CCGAAACAGA ACTGCCCAAC GATGTCCAAT CAACAAGCTC GGTGCTGAGC 
ACACACACGG AGAAGAAGGA CAAAGCAAAG GAGGATCTGC AGCTGGCACG GAAAGAGGGG 
AACAGCATCC TTGAGAGCCT CAGGGAGCCA CTGGCTGAAA GCACTGCCCA CAGTGTAAAC 
CAAGACCAGC TGGACAATCA GGCCACAGTG CAGAGGCTCC TGGCTCAGCT GAATGAAACG 
GAGGCCGCCT TTGACGAGTT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAGGG TTTCCGGGAG GTCAAAACTA CCTTGGATAC CATGTCCCAC 
AAGATAGCCA CCTTCACTGA CGTTGGTAAC AGCCTGGCAC ATGTGCAACA CCTGCTGAAG 
GACCTGGCCA GCTTCGAAGA GAAGTCTAGC GTGGCTGTAG ACAGGGCCCG AGCCCTGTCA 
CTGGAGGGTC AGCAGCTGAT TGAGAACAAG CACTATGCTG TGGACTCCAT CCACCCTAAG 
TGTGAGGAGC TCCAGCACCT CTGCGATCAC TTCACCGCTG AGATCAGCAG GAGGAGAGGC 
TTGCTCAGCA AGTCCCTGGA GCTACACAGC CTCTTGGAGA CGTCCATGAA GTGGTGCGAT 
GAGGGCATCT TCCTGCTGGC CTCGCAACCT GTGGACAAGT GTCAGTCCCA GGATGGTGCT 
GAGGCAGCCC TCCAGGAGAT CGAGAAATTT TTGGAGACTG GTGCAGAAAA TAAGATTCAA 
GAACTCAATA AAATTTACAA GGAATATGAG TGCATCCTCA ACCAGGACCT ACTGGAACAC 
GTGCAAAAGG TCTTTCAGAA GCAAGAAAGT ATGCAAGAGA TGTTCCACCG CAGGCAGGCC 
AGCCTGAAGA AGCTGGCGGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCACGGCCA 
GAGGCACTCA CCAAATCACC CTGCCCCTCC CCAGGCTCCT GGAGAGGATC TGAGAACTCT 
AGCTCTGAGG GCAGTGCACT CCGTAGAGGG CCCTACAGGA GAGCCAAGAG TGAAATGAGT 
GAGACCCGGC AGGGCCGTGC CAGCTCCACA GGGGACGAGG AGGAGAGCCT AGCCATCCTG 
CGCAGGCATG TGATGAATGA GCTCCTAGAC ACGGAACGGG CCTACGTGGA GGAGCTGCTC 
TGTGTACTAG AGGGCTATGC TGCAGAGATG GACAACCCCC TGATGGCACA CCTCATTTCA 
GCAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAGAT TTATCACTTT 
CATAACAGGA TATTCCTGCG GGAGCTAGAG AGCTGCATAG ACTGCCCGGA ACTGGTGGGG 
AGGTGCTTTC TGGAGAGGAT GGAGGAGTTC CAGATCTACG AGAAGTACTG TCAGAACAAG 
CCACGATCTG AGAGCCTGTG GAGACAGTGC TCTGACTGTC CCTTCTTCCA GGAGTGCCAG 
AAGAAGTTGG ACCACAAACT GAGCCTTGAT TCCTACCTGC TGAAGCCAGT GCAGAGAATA 
ACTAAGTACC AGTTGCTGCT TAAGGAAATG TTGAAATACA GCAAGAACTG TGAGGGGGCA 
GAGGACCTAC AGGAGGCACT GAGCTCCATC CTGGGCATCC TCAAGGCAGT GAATGACTCC 
ATGCACCTCA TCGCCATCAC TGGCTACGAC GGTAACCTGG GTGATCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGTGT ATGGACAGAC CACAAGAAGG GCCACACCAA GGTCAAGGAG 
CTGGCCAGAT TCAAGCCCAT GCAGCGGCAC CTTTTCCTGC ATGAGAAGGC TGTGCTGTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGG TATGAGAAGG CCCCTTCCTA CAGCTACAAG 
CAGTCATTGA ATATGACTGC TGTTGGCATC ACAGAGAATG TGAAGGGTGA TGCCAAGAAG 
TTCGAGATCT GGTACAACGC AAGAGAGGAG GTTTACATCA TCCAGGCACC AACCCCTGAG 
ATTAAAGCAG CATGGGTGAG TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCCTGC 
CGGGAAGCCA GCCAGCACAG GGCCCTGGAG CAGTCCCACA GCCTGCCTTT GCCCACACCA 
TCCAACACCA GCCCCTCAAA AGGGAACACA AGGAATGTCA AGAAGTCAGA AGAAAGAAAA 
ACGGACCCCC TGAGCCTGGA AGGATATGTG AGCTCATCGT TGCCGAAGCC CCCTGAGAAA 
GGCAAAGACG ATGCGGTCCC TAGTTCTACC TCTGAAAGCT CTGCGCTTTC CAGAAAGCGC 
TTCACGCTGC AGGGCTTGGC TAACCTCAAA GGTCAGAAAG CCTCTCCGAC CAGTCCTGAC 
AAAAAAGCTA AGCGGCACGA AGTAAAGAGC GACCCGACTC CCTTTGGTTT GCGAGGCTGG 
AGCAAAACAT CCCACTCCCT GGAAGCCCCT GAGGAGGACG GAGGTTGGTC CAGTGCTGAG 
GAGCTTGTCA ATTCATCTGA TGCAGAGGAA GATGGAGTGG GCCCCAAGAA GCTGGTTCCT 
GGTAAATACA CAGTGGTGAT GGACGATGAG AAAGGGGGCC TTGACACCCT TGCTGTGAGG 
AGCGGGGACA TGGTAGAGGT GGTGGAAGAG GGCGCAGAGG GGCTCTGGTA TGTCCGGGAC 
CTGACCAGCA GCAAGGAGGG ATGGGTGCCA GCCAGCAGCC TTTCCACCCT CCTGGGCAAA 
TCCAGTTCAG CCCAGTGTCT GAGCAGCTCA GACTCCAGCC CAGGGTCAGC CGTGCTGAGC 
AACTCCTCCA GCTGCAGCGA GGGCTGCCCC ACTCCCTTCT CCGACCTCCA GGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848411.3 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846633.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X7, mRNA.

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CloneID	ONa31077
Clone ID Related Accession (Same CDS sequence)	XM_008848411.2 , XM_008848411.3
Accession Version	XM_008848411.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008848411.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTGA GCCTCATCTC CGTGCTGTCC CAGGACGTCA CAGCCCTGTG GCTCCTGCTG 
AAGACAAGTG CAGATGAAAT CATGCACCAG GACATCGTCC CACTCTGTGC TGCGGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGGCA GGACGGAAGC 
CCAGTTATCA CCTTTCCAGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATT 
CTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCCGTGA AGGCGTCGGT CCTGCGAATA 
GCCGCATCTT TCCCAGCAAA CCTGCAGCTC GTCCTTGTGC TTCGCCCAAC GGGCTTTTTC 
CAGAGGACTC TCTCTGACCT CGCCTTCAAG TTCAACAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATGA TGCTGAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCACAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCATTCCA GGTGGCTGTG CCACCGCACG 
GCGATCGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCTC AGATGCTGCA GGCCTTCGGG 
ACCGAGCTGG CCGAAACAGA ACTGCCCAAC GATGTCCAAT CAACAAGCTC GGTGCTGAGC 
ACACACACGG AGAAGAAGGA CAAAGCAAAG GAGGATCTGC AGCTGGCACG GAAAGAGGGG 
AACAGCATCC TTGAGAGCCT CAGGGAGCCA CTGGCTGAAA GCACTGCCCA CAGTGTAAAC 
CAAGACCAGC TGGACAATCA GGCCACAGTG CAGAGGCTCC TGGCTCAGCT GAATGAAACG 
GAGGCCGCCT TTGACGAGTT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAGGG TTTCCGGGAG GTCAAAACTA CCTTGGATAC CATGTCCCAC 
AAGATAGCCA CCTTCACTGA CGTTGGTAAC AGCCTGGCAC ATGTGCAACA CCTGCTGAAG 
GACCTGGCCA GCTTCGAAGA GAAGTCTAGC GTGGCTGTAG ACAGGGCCCG AGCCCTGTCA 
CTGGAGGGTC AGCAGCTGAT TGAGAACAAG CACTATGCTG TGGACTCCAT CCACCCTAAG 
TGTGAGGAGC TCCAGCACCT CTGCGATCAC TTCACCGCTG AGATCAGCAG GAGGAGAGGC 
TTGCTCAGCA AGTCCCTGGA GCTACACAGC CTCTTGGAGA CGTCCATGAA GTGGTGCGAT 
GAGGGCATCT TCCTGCTGGC CTCGCAACCT GTGGACAAGT GTCAGTCCCA GGATGGTGCT 
GAGGCAGCCC TCCAGGAGAT CGAGAAATTT TTGGAGACTG GTGCAGAAAA TAAGATTCAA 
GAACTCAATA AAATTTACAA GGAATATGAG TGCATCCTCA ACCAGGACCT ACTGGAACAC 
GTGCAAAAGG TCTTTCAGAA GCAAGAAAGT ATGCAAGAGA TGTTCCACCG CAGGCAGGCC 
AGCCTGAAGA AGCTGGCGGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCACGGCCA 
GAGGCACTCA CCAAATCACC CTGCCCCTCC CCAGGCTCCT GGAGAGGATC TGAGAACTCT 
AGCTCTGAGG GCAGTGCACT CCGTAGAGGG CCCTACAGGA GAGCCAAGAG TGAAATGAGT 
GAGACCCGGC AGGGCCGTGC CAGCTCCACA GGGGACGAGG AGGAGAGCCT AGCCATCCTG 
CGCAGGCATG TGATGAATGA GCTCCTAGAC ACGGAACGGG CCTACGTGGA GGAGCTGCTC 
TGTGTACTAG AGGGCTATGC TGCAGAGATG GACAACCCCC TGATGGCACA CCTCATTTCA 
GCAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAGAT TTATCACTTT 
CATAACAGGA TATTCCTGCG GGAGCTAGAG AGCTGCATAG ACTGCCCGGA ACTGGTGGGG 
AGGTGCTTTC TGGAGAGGAT GGAGGAGTTC CAGATCTACG AGAAGTACTG TCAGAACAAG 
CCACGATCTG AGAGCCTGTG GAGACAGTGC TCTGACTGTC CCTTCTTCCA GGAGTGCCAG 
AAGAAGTTGG ACCACAAACT GAGCCTTGAT TCCTACCTGC TGAAGCCAGT GCAGAGAATA 
ACTAAGTACC AGTTGCTGCT TAAGGAAATG TTGAAATACA GCAAGAACTG TGAGGGGGCA 
GAGGACCTAC AGGAGGCACT GAGCTCCATC CTGGGCATCC TCAAGGCAGT GAATGACTCC 
ATGCACCTCA TCGCCATCAC TGGCTACGAC GGTAACCTGG GTGATCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGTGT ATGGACAGAC CACAAGAAGG GCCACACCAA GGTCAAGGAG 
CTGGCCAGAT TCAAGCCCAT GCAGCGGCAC CTTTTCCTGC ATGAGAAGGC TGTGCTGTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGG TATGAGAAGG CCCCTTCCTA CAGCTACAAG 
CAGTCATTGA ATATGACTGC TGTTGGCATC ACAGAGAATG TGAAGGGTGA TGCCAAGAAG 
TTCGAGATCT GGTACAACGC AAGAGAGGAG GTTTACATCA TCCAGGCACC AACCCCTGAG 
ATTAAAGCAG CATGGGTGAG TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCCTGC 
CGGGAAGCCA GCCAGCACAG GGCCCTGGAG CAGTCCCACA GCCTGCCTTT GCCCACACCA 
TCCAACACCA GCCCCTCAAA AGGGAACACA AGGAATGTCA AGAAGTCAGA AGAAAGAAAA 
ACGGACCCCC TGAGCCTGGA AGGATATGTG AGCTCATCGT TGCCGAAGCC CCCTGAGAAA 
GGCAAAGACG ATGCGGTCCC TAGTTCTACC TCTGAAAGCT CTGCGCTTTC CAGAAAGCGC 
TTCACGCTGC AGGGCTTGGC TAACCTCAAA GGTCAGAAAG CCTCTCCGAC CAGTCCTGAC 
AAAAAAGCTA AGCGGCACGA AGTAAAGAGC GACCCGACTC CCTTTGGTTT GCGAGGCTGG 
AGCAAAACAT CCCACTCCCT GGAAGCCCCT GAGGAGGACG GAGGTTGGTC CAGTGCTGAG 
GAGCTTGTCA ATTCATCTGA TGCAGAGGAA GATGGAGTGG GCCCCAAGAA GCTGGTTCCT 
GGTAAATACA CAGTGGTGAT GGACGATGAG AAAGGGGGCC TTGACACCCT TGCTGTGAGG 
AGCGGGGACA TGGTAGAGGT GGTGGAAGAG GGCGCAGAGG GGCTCTGGTA TGTCCGGGAC 
CTGACCAGCA GCAAGGAGGG ATGGGTGCCA GCCAGCAGCC TTTCCACCCT CCTGGGCAAA 
TCCAGTTCAG CCCAGTGTCT GAGCAGCTCA GACTCCAGCC CAGGGTCAGC CGTGCTGAGC 
AACTCCTCCA GCTGCAGCGA GGGCTGCCCC ACTCCCTTCT CCGACCTCCA GGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846633.1
CDS	102..3638
Translation	MTLSLISVLSQDVTALWLLLKTSADEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_008848411.3
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848411.3

ORF Insert Sequence:

ATGACTTTGA GCCTCATCTC CGTGCTGTCC CAGGACGTCA CAGCCCTGTG GCTCCTGCTG 
AAGACAAGTG CAGATGAAAT CATGCACCAG GACATCGTCC CACTCTGTGC TGCGGACATC 
CAGGAGCAGC TGAAGAAGCG CTTTGCTTAC CTGTCTGGTG GGCGGGGGCA GGACGGAAGC 
CCAGTTATCA CCTTTCCAGA CTACCCAGCC TTCAGCGAGA TCCCGGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGTTTGCAAG ATGCTGGCAT TGGATTCATT 
CTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCCGTGA AGGCGTCGGT CCTGCGAATA 
GCCGCATCTT TCCCAGCAAA CCTGCAGCTC GTCCTTGTGC TTCGCCCAAC GGGCTTTTTC 
CAGAGGACTC TCTCTGACCT CGCCTTCAAG TTCAACAGAG ATGACTTTAA GATGAAGGTG 
CCGGTCATGA TGCTGAGCTC AGTGCCAGAA CTACACGGCT ACATTGATAA GTCACAGCTG 
ACCGAGGACC TCGGGGGGAC CCTGGACTAC TGCCATTCCA GGTGGCTGTG CCACCGCACG 
GCGATCGAAA GCTTCGCCCT GATGGTGAAG CAGACAGCTC AGATGCTGCA GGCCTTCGGG 
ACCGAGCTGG CCGAAACAGA ACTGCCCAAC GATGTCCAAT CAACAAGCTC GGTGCTGAGC 
ACACACACGG AGAAGAAGGA CAAAGCAAAG GAGGATCTGC AGCTGGCACG GAAAGAGGGG 
AACAGCATCC TTGAGAGCCT CAGGGAGCCA CTGGCTGAAA GCACTGCCCA CAGTGTAAAC 
CAAGACCAGC TGGACAATCA GGCCACAGTG CAGAGGCTCC TGGCTCAGCT GAATGAAACG 
GAGGCCGCCT TTGACGAGTT CTGGGCAAAG CATCAGCAGA AGCTGGAACA GTGCCTGCAG 
CTGCGGCATT TTGAGCAGGG TTTCCGGGAG GTCAAAACTA CCTTGGATAC CATGTCCCAC 
AAGATAGCCA CCTTCACTGA CGTTGGTAAC AGCCTGGCAC ATGTGCAACA CCTGCTGAAG 
GACCTGGCCA GCTTCGAAGA GAAGTCTAGC GTGGCTGTAG ACAGGGCCCG AGCCCTGTCA 
CTGGAGGGTC AGCAGCTGAT TGAGAACAAG CACTATGCTG TGGACTCCAT CCACCCTAAG 
TGTGAGGAGC TCCAGCACCT CTGCGATCAC TTCACCGCTG AGATCAGCAG GAGGAGAGGC 
TTGCTCAGCA AGTCCCTGGA GCTACACAGC CTCTTGGAGA CGTCCATGAA GTGGTGCGAT 
GAGGGCATCT TCCTGCTGGC CTCGCAACCT GTGGACAAGT GTCAGTCCCA GGATGGTGCT 
GAGGCAGCCC TCCAGGAGAT CGAGAAATTT TTGGAGACTG GTGCAGAAAA TAAGATTCAA 
GAACTCAATA AAATTTACAA GGAATATGAG TGCATCCTCA ACCAGGACCT ACTGGAACAC 
GTGCAAAAGG TCTTTCAGAA GCAAGAAAGT ATGCAAGAGA TGTTCCACCG CAGGCAGGCC 
AGCCTGAAGA AGCTGGCGGC CAAGCAGACA CGGCCTGTGC AGCCCGTGGC CCCACGGCCA 
GAGGCACTCA CCAAATCACC CTGCCCCTCC CCAGGCTCCT GGAGAGGATC TGAGAACTCT 
AGCTCTGAGG GCAGTGCACT CCGTAGAGGG CCCTACAGGA GAGCCAAGAG TGAAATGAGT 
GAGACCCGGC AGGGCCGTGC CAGCTCCACA GGGGACGAGG AGGAGAGCCT AGCCATCCTG 
CGCAGGCATG TGATGAATGA GCTCCTAGAC ACGGAACGGG CCTACGTGGA GGAGCTGCTC 
TGTGTACTAG AGGGCTATGC TGCAGAGATG GACAACCCCC TGATGGCACA CCTCATTTCA 
GCAGGCCTGC AAAACAAGAA GAACATTCTG TTTGGAAACA TGGAGGAGAT TTATCACTTT 
CATAACAGGA TATTCCTGCG GGAGCTAGAG AGCTGCATAG ACTGCCCGGA ACTGGTGGGG 
AGGTGCTTTC TGGAGAGGAT GGAGGAGTTC CAGATCTACG AGAAGTACTG TCAGAACAAG 
CCACGATCTG AGAGCCTGTG GAGACAGTGC TCTGACTGTC CCTTCTTCCA GGAGTGCCAG 
AAGAAGTTGG ACCACAAACT GAGCCTTGAT TCCTACCTGC TGAAGCCAGT GCAGAGAATA 
ACTAAGTACC AGTTGCTGCT TAAGGAAATG TTGAAATACA GCAAGAACTG TGAGGGGGCA 
GAGGACCTAC AGGAGGCACT GAGCTCCATC CTGGGCATCC TCAAGGCAGT GAATGACTCC 
ATGCACCTCA TCGCCATCAC TGGCTACGAC GGTAACCTGG GTGATCTGGG GAAGCTGCTG 
ATGCAAGGTT CCTTCAGTGT ATGGACAGAC CACAAGAAGG GCCACACCAA GGTCAAGGAG 
CTGGCCAGAT TCAAGCCCAT GCAGCGGCAC CTTTTCCTGC ATGAGAAGGC TGTGCTGTTC 
TGCAAGAAGA GGGAGGAGAA TGGGGAGGGG TATGAGAAGG CCCCTTCCTA CAGCTACAAG 
CAGTCATTGA ATATGACTGC TGTTGGCATC ACAGAGAATG TGAAGGGTGA TGCCAAGAAG 
TTCGAGATCT GGTACAACGC AAGAGAGGAG GTTTACATCA TCCAGGCACC AACCCCTGAG 
ATTAAAGCAG CATGGGTGAG TGAGATTCGG AAGGTGCTGA CCAGCCAGCT GCAGGCCTGC 
CGGGAAGCCA GCCAGCACAG GGCCCTGGAG CAGTCCCACA GCCTGCCTTT GCCCACACCA 
TCCAACACCA GCCCCTCAAA AGGGAACACA AGGAATGTCA AGAAGTCAGA AGAAAGAAAA 
ACGGACCCCC TGAGCCTGGA AGGATATGTG AGCTCATCGT TGCCGAAGCC CCCTGAGAAA 
GGCAAAGACG ATGCGGTCCC TAGTTCTACC TCTGAAAGCT CTGCGCTTTC CAGAAAGCGC 
TTCACGCTGC AGGGCTTGGC TAACCTCAAA GGTCAGAAAG CCTCTCCGAC CAGTCCTGAC 
AAAAAAGCTA AGCGGCACGA AGTAAAGAGC GACCCGACTC CCTTTGGTTT GCGAGGCTGG 
AGCAAAACAT CCCACTCCCT GGAAGCCCCT GAGGAGGACG GAGGTTGGTC CAGTGCTGAG 
GAGCTTGTCA ATTCATCTGA TGCAGAGGAA GATGGAGTGG GCCCCAAGAA GCTGGTTCCT 
GGTAAATACA CAGTGGTGAT GGACGATGAG AAAGGGGGCC TTGACACCCT TGCTGTGAGG 
AGCGGGGACA TGGTAGAGGT GGTGGAAGAG GGCGCAGAGG GGCTCTGGTA TGTCCGGGAC 
CTGACCAGCA GCAAGGAGGG ATGGGTGCCA GCCAGCAGCC TTTCCACCCT CCTGGGCAAA 
TCCAGTTCAG CCCAGTGTCT GAGCAGCTCA GACTCCAGCC CAGGGTCAGC CGTGCTGAGC 
AACTCCTCCA GCTGCAGCGA GGGCTGCCCC ACTCCCTTCT CCGACCTCCA GGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848414.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846636.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X7, mRNA.

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CloneID	ONa31078
Clone ID Related Accession (Same CDS sequence)	XM_008848414.1 , XM_008848414.2
Accession Version	XM_008848414.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACCTC ATGGAGAGCG TGCGCTGGGC TGCTGCGGCC TCTGCACCTG CCCGGCAGCA 
GCAGGAGATG AAATCATGCA CCAGGACATC GTCCCACTCT GTGCTGCGGA CATCCAGGAG 
CAGCTGAAGA AGCGCTTTGC TTACCTGTCT GGTGGGCGGG GGCAGGACGG AAGCCCAGTT 
ATCACCTTTC CAGACTACCC AGCCTTCAGC GAGATCCCGG ACAAGGAGTT CCAGAATGTC 
ATGACCTATC TCACCAGCAT CCCCAGTTTG CAAGATGCTG GCATTGGATT CATTCTGGTC 
ATAGACCGAA GGCAGGACAA ATGGACCTCC GTGAAGGCGT CGGTCCTGCG AATAGCCGCA 
TCTTTCCCAG CAAACCTGCA GCTCGTCCTT GTGCTTCGCC CAACGGGCTT TTTCCAGAGG 
ACTCTCTCTG ACCTCGCCTT CAAGTTCAAC AGAGATGACT TTAAGATGAA GGTGCCGGTC 
ATGATGCTGA GCTCAGTGCC AGAACTACAC GGCTACATTG ATAAGTCACA GCTGACCGAG 
GACCTCGGGG GGACCCTGGA CTACTGCCAT TCCAGGTGGC TGTGCCACCG CACGGCGATC 
GAAAGCTTCG CCCTGATGGT GAAGCAGACA GCTCAGATGC TGCAGGCCTT CGGGACCGAG 
CTGGCCGAAA CAGAACTGCC CAACGATGTC CAATCAACAA GCTCGGTGCT GAGCACACAC 
ACGGAGAAGA AGGACAAAGC AAAGGAGGAT CTGCAGCTGG CACGGAAAGA GGGGAACAGC 
ATCCTTGAGA GCCTCAGGGA GCCACTGGCT GAAAGCACTG CCCACAGTGT AAACCAAGAC 
CAGCTGGACA ATCAGGCCAC AGTGCAGAGG CTCCTGGCTC AGCTGAATGA AACGGAGGCC 
GCCTTTGACG AGTTCTGGGC AAAGCATCAG CAGAAGCTGG AACAGTGCCT GCAGCTGCGG 
CATTTTGAGC AGGGTTTCCG GGAGGTCAAA ACTACCTTGG ATACCATGTC CCACAAGATA 
GCCACCTTCA CTGACGTTGG TAACAGCCTG GCACATGTGC AACACCTGCT GAAGGACCTG 
GCCAGCTTCG AAGAGAAGTC TAGCGTGGCT GTAGACAGGG CCCGAGCCCT GTCACTGGAG 
GGTCAGCAGC TGATTGAGAA CAAGCACTAT GCTGTGGACT CCATCCACCC TAAGTGTGAG 
GAGCTCCAGC ACCTCTGCGA TCACTTCACC GCTGAGATCA GCAGGAGGAG AGGCTTGCTC 
AGCAAGTCCC TGGAGCTACA CAGCCTCTTG GAGACGTCCA TGAAGTGGTG CGATGAGGGC 
ATCTTCCTGC TGGCCTCGCA ACCTGTGGAC AAGTGTCAGT CCCAGGATGG TGCTGAGGCA 
GCCCTCCAGG AGATCGAGAA ATTTTTGGAG ACTGGTGCAG AAAATAAGAT TCAAGAACTC 
AATAAAATTT ACAAGGAATA TGAGTGCATC CTCAACCAGG ACCTACTGGA ACACGTGCAA 
AAGGTCTTTC AGAAGCAAGA AAGTATGCAA GAGATGTTCC ACCGCAGGCA GGCCAGCCTG 
AAGAAGCTGG CGGCCAAGCA GACACGGCCT GTGCAGCCCG TGGCCCCACG GCCAGAGGCA 
CTCACCAAAT CACCCTGCCC CTCCCCAGGC TCCTGGAGAG GATCTGAGAA CTCTAGCTCT 
GAGGGCAGTG CACTCCGTAG AGGGCCCTAC AGGAGAGCCA AGAGTGAAAT GAGTGAGACC 
CGGCAGGGCC GTGCCAGCTC CACAGGGGAC GAGGAGGAGA GCCTAGCCAT CCTGCGCAGG 
CATGTGATGA ATGAGCTCCT AGACACGGAA CGGGCCTACG TGGAGGAGCT GCTCTGTGTA 
CTAGAGGGCT ATGCTGCAGA GATGGACAAC CCCCTGATGG CACACCTCAT TTCAGCAGGC 
CTGCAAAACA AGAAGAACAT TCTGTTTGGA AACATGGAGG AGATTTATCA CTTTCATAAC 
AGGATATTCC TGCGGGAGCT AGAGAGCTGC ATAGACTGCC CGGAACTGGT GGGGAGGTGC 
TTTCTGGAGA GGATGGAGGA GTTCCAGATC TACGAGAAGT ACTGTCAGAA CAAGCCACGA 
TCTGAGAGCC TGTGGAGACA GTGCTCTGAC TGTCCCTTCT TCCAGGAGTG CCAGAAGAAG 
TTGGACCACA AACTGAGCCT TGATTCCTAC CTGCTGAAGC CAGTGCAGAG AATAACTAAG 
TACCAGTTGC TGCTTAAGGA AATGTTGAAA TACAGCAAGA ACTGTGAGGG GGCAGAGGAC 
CTACAGGAGG CACTGAGCTC CATCCTGGGC ATCCTCAAGG CAGTGAATGA CTCCATGCAC 
CTCATCGCCA TCACTGGCTA CGACGGTAAC CTGGGTGATC TGGGGAAGCT GCTGATGCAA 
GGTTCCTTCA GTGTATGGAC AGACCACAAG AAGGGCCACA CCAAGGTCAA GGAGCTGGCC 
AGATTCAAGC CCATGCAGCG GCACCTTTTC CTGCATGAGA AGGCTGTGCT GTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGGTATGAG AAGGCCCCTT CCTACAGCTA CAAGCAGTCA 
TTGAATATGA CTGCTGTTGG CATCACAGAG AATGTGAAGG GTGATGCCAA GAAGTTCGAG 
ATCTGGTACA ACGCAAGAGA GGAGGTTTAC ATCATCCAGG CACCAACCCC TGAGATTAAA 
GCAGCATGGG TGAGTGAGAT TCGGAAGGTG CTGACCAGCC AGCTGCAGGC CTGCCGGGAA 
GCCAGCCAGC ACAGGGCCCT GGAGCAGTCC CACAGCCTGC CTTTGCCCAC ACCATCCAAC 
ACCAGCCCCT CAAAAGGGAA CACAAGGAAT GTCAAGAAGT CAGAAGAAAG AAAAACGGAC 
CCCCTGAGCC TGGAAGGATA TGTGAGCTCA TCGTTGCCGA AGCCCCCTGA GAAAGGCAAA 
GACGATGCGG TCCCTAGTTC TACCTCTGAA AGCTCTGCGC TTTCCAGAAA GCGCTTCACG 
CTGCAGGGCT TGGCTAACCT CAAAGGTCAG AAAGCCTCTC CGACCAGTCC TGACAAAAAA 
GCTAAGCGGC ACGAAGTAAA GAGCGACCCG ACTCCCTTTG GTTTGCGAGG CTGGAGCAAA 
ACATCCCACT CCCTGGAAGC CCCTGAGGAG GACGGAGGTT GGTCCAGTGC TGAGGAGCTT 
GTCAATTCAT CTGATGCAGA GGAAGATGGA GTGGGCCCCA AGAAGCTGGT TCCTGGTAAA 
TACACAGTGG TGATGGACGA TGAGAAAGGG GGCCTTGACA CCCTTGCTGT GAGGAGCGGG 
GACATGGTAG AGGTGGTGGA AGAGGGCGCA GAGGGGCTCT GGTATGTCCG GGACCTGACC 
AGCAGCAAGG AGGGATGGGT GCCAGCCAGC AGCCTTTCCA CCCTCCTGGG CAAATCCAGT 
TCAGCCCAGT GTCTGAGCAG CTCAGACTCC AGCCCAGGGT CAGCCGTGCT GAGCAACTCC 
TCCAGCTGCA GCGAGGGCTG CCCCACTCCC TTCTCCGACC TCCAGGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846636.1
CDS	125..3655
Translation	MRPHGERALGCCGLCTCPAAAGDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_008848414.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848414.1

ORF Insert Sequence:

ATGAGACCTC ATGGAGAGCG TGCGCTGGGC TGCTGCGGCC TCTGCACCTG CCCGGCAGCA 
GCAGGAGATG AAATCATGCA CCAGGACATC GTCCCACTCT GTGCTGCGGA CATCCAGGAG 
CAGCTGAAGA AGCGCTTTGC TTACCTGTCT GGTGGGCGGG GGCAGGACGG AAGCCCAGTT 
ATCACCTTTC CAGACTACCC AGCCTTCAGC GAGATCCCGG ACAAGGAGTT CCAGAATGTC 
ATGACCTATC TCACCAGCAT CCCCAGTTTG CAAGATGCTG GCATTGGATT CATTCTGGTC 
ATAGACCGAA GGCAGGACAA ATGGACCTCC GTGAAGGCGT CGGTCCTGCG AATAGCCGCA 
TCTTTCCCAG CAAACCTGCA GCTCGTCCTT GTGCTTCGCC CAACGGGCTT TTTCCAGAGG 
ACTCTCTCTG ACCTCGCCTT CAAGTTCAAC AGAGATGACT TTAAGATGAA GGTGCCGGTC 
ATGATGCTGA GCTCAGTGCC AGAACTACAC GGCTACATTG ATAAGTCACA GCTGACCGAG 
GACCTCGGGG GGACCCTGGA CTACTGCCAT TCCAGGTGGC TGTGCCACCG CACGGCGATC 
GAAAGCTTCG CCCTGATGGT GAAGCAGACA GCTCAGATGC TGCAGGCCTT CGGGACCGAG 
CTGGCCGAAA CAGAACTGCC CAACGATGTC CAATCAACAA GCTCGGTGCT GAGCACACAC 
ACGGAGAAGA AGGACAAAGC AAAGGAGGAT CTGCAGCTGG CACGGAAAGA GGGGAACAGC 
ATCCTTGAGA GCCTCAGGGA GCCACTGGCT GAAAGCACTG CCCACAGTGT AAACCAAGAC 
CAGCTGGACA ATCAGGCCAC AGTGCAGAGG CTCCTGGCTC AGCTGAATGA AACGGAGGCC 
GCCTTTGACG AGTTCTGGGC AAAGCATCAG CAGAAGCTGG AACAGTGCCT GCAGCTGCGG 
CATTTTGAGC AGGGTTTCCG GGAGGTCAAA ACTACCTTGG ATACCATGTC CCACAAGATA 
GCCACCTTCA CTGACGTTGG TAACAGCCTG GCACATGTGC AACACCTGCT GAAGGACCTG 
GCCAGCTTCG AAGAGAAGTC TAGCGTGGCT GTAGACAGGG CCCGAGCCCT GTCACTGGAG 
GGTCAGCAGC TGATTGAGAA CAAGCACTAT GCTGTGGACT CCATCCACCC TAAGTGTGAG 
GAGCTCCAGC ACCTCTGCGA TCACTTCACC GCTGAGATCA GCAGGAGGAG AGGCTTGCTC 
AGCAAGTCCC TGGAGCTACA CAGCCTCTTG GAGACGTCCA TGAAGTGGTG CGATGAGGGC 
ATCTTCCTGC TGGCCTCGCA ACCTGTGGAC AAGTGTCAGT CCCAGGATGG TGCTGAGGCA 
GCCCTCCAGG AGATCGAGAA ATTTTTGGAG ACTGGTGCAG AAAATAAGAT TCAAGAACTC 
AATAAAATTT ACAAGGAATA TGAGTGCATC CTCAACCAGG ACCTACTGGA ACACGTGCAA 
AAGGTCTTTC AGAAGCAAGA AAGTATGCAA GAGATGTTCC ACCGCAGGCA GGCCAGCCTG 
AAGAAGCTGG CGGCCAAGCA GACACGGCCT GTGCAGCCCG TGGCCCCACG GCCAGAGGCA 
CTCACCAAAT CACCCTGCCC CTCCCCAGGC TCCTGGAGAG GATCTGAGAA CTCTAGCTCT 
GAGGGCAGTG CACTCCGTAG AGGGCCCTAC AGGAGAGCCA AGAGTGAAAT GAGTGAGACC 
CGGCAGGGCC GTGCCAGCTC CACAGGGGAC GAGGAGGAGA GCCTAGCCAT CCTGCGCAGG 
CATGTGATGA ATGAGCTCCT AGACACGGAA CGGGCCTACG TGGAGGAGCT GCTCTGTGTA 
CTAGAGGGCT ATGCTGCAGA GATGGACAAC CCCCTGATGG CACACCTCAT TTCAGCAGGC 
CTGCAAAACA AGAAGAACAT TCTGTTTGGA AACATGGAGG AGATTTATCA CTTTCATAAC 
AGGATATTCC TGCGGGAGCT AGAGAGCTGC ATAGACTGCC CGGAACTGGT GGGGAGGTGC 
TTTCTGGAGA GGATGGAGGA GTTCCAGATC TACGAGAAGT ACTGTCAGAA CAAGCCACGA 
TCTGAGAGCC TGTGGAGACA GTGCTCTGAC TGTCCCTTCT TCCAGGAGTG CCAGAAGAAG 
TTGGACCACA AACTGAGCCT TGATTCCTAC CTGCTGAAGC CAGTGCAGAG AATAACTAAG 
TACCAGTTGC TGCTTAAGGA AATGTTGAAA TACAGCAAGA ACTGTGAGGG GGCAGAGGAC 
CTACAGGAGG CACTGAGCTC CATCCTGGGC ATCCTCAAGG CAGTGAATGA CTCCATGCAC 
CTCATCGCCA TCACTGGCTA CGACGGTAAC CTGGGTGATC TGGGGAAGCT GCTGATGCAA 
GGTTCCTTCA GTGTATGGAC AGACCACAAG AAGGGCCACA CCAAGGTCAA GGAGCTGGCC 
AGATTCAAGC CCATGCAGCG GCACCTTTTC CTGCATGAGA AGGCTGTGCT GTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGGTATGAG AAGGCCCCTT CCTACAGCTA CAAGCAGTCA 
TTGAATATGA CTGCTGTTGG CATCACAGAG AATGTGAAGG GTGATGCCAA GAAGTTCGAG 
ATCTGGTACA ACGCAAGAGA GGAGGTTTAC ATCATCCAGG CACCAACCCC TGAGATTAAA 
GCAGCATGGG TGAGTGAGAT TCGGAAGGTG CTGACCAGCC AGCTGCAGGC CTGCCGGGAA 
GCCAGCCAGC ACAGGGCCCT GGAGCAGTCC CACAGCCTGC CTTTGCCCAC ACCATCCAAC 
ACCAGCCCCT CAAAAGGGAA CACAAGGAAT GTCAAGAAGT CAGAAGAAAG AAAAACGGAC 
CCCCTGAGCC TGGAAGGATA TGTGAGCTCA TCGTTGCCGA AGCCCCCTGA GAAAGGCAAA 
GACGATGCGG TCCCTAGTTC TACCTCTGAA AGCTCTGCGC TTTCCAGAAA GCGCTTCACG 
CTGCAGGGCT TGGCTAACCT CAAAGGTCAG AAAGCCTCTC CGACCAGTCC TGACAAAAAA 
GCTAAGCGGC ACGAAGTAAA GAGCGACCCG ACTCCCTTTG GTTTGCGAGG CTGGAGCAAA 
ACATCCCACT CCCTGGAAGC CCCTGAGGAG GACGGAGGTT GGTCCAGTGC TGAGGAGCTT 
GTCAATTCAT CTGATGCAGA GGAAGATGGA GTGGGCCCCA AGAAGCTGGT TCCTGGTAAA 
TACACAGTGG TGATGGACGA TGAGAAAGGG GGCCTTGACA CCCTTGCTGT GAGGAGCGGG 
GACATGGTAG AGGTGGTGGA AGAGGGCGCA GAGGGGCTCT GGTATGTCCG GGACCTGACC 
AGCAGCAAGG AGGGATGGGT GCCAGCCAGC AGCCTTTCCA CCCTCCTGGG CAAATCCAGT 
TCAGCCCAGT GTCTGAGCAG CTCAGACTCC AGCCCAGGGT CAGCCGTGCT GAGCAACTCC 
TCCAGCTGCA GCGAGGGCTG CCCCACTCCC TTCTCCGACC TCCAGGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848414.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846636.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X8, mRNA.

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CloneID	ONa31078
Clone ID Related Accession (Same CDS sequence)	XM_008848414.1 , XM_008848414.2
Accession Version	XM_008848414.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008848414.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACCTC ATGGAGAGCG TGCGCTGGGC TGCTGCGGCC TCTGCACCTG CCCGGCAGCA 
GCAGGAGATG AAATCATGCA CCAGGACATC GTCCCACTCT GTGCTGCGGA CATCCAGGAG 
CAGCTGAAGA AGCGCTTTGC TTACCTGTCT GGTGGGCGGG GGCAGGACGG AAGCCCAGTT 
ATCACCTTTC CAGACTACCC AGCCTTCAGC GAGATCCCGG ACAAGGAGTT CCAGAATGTC 
ATGACCTATC TCACCAGCAT CCCCAGTTTG CAAGATGCTG GCATTGGATT CATTCTGGTC 
ATAGACCGAA GGCAGGACAA ATGGACCTCC GTGAAGGCGT CGGTCCTGCG AATAGCCGCA 
TCTTTCCCAG CAAACCTGCA GCTCGTCCTT GTGCTTCGCC CAACGGGCTT TTTCCAGAGG 
ACTCTCTCTG ACCTCGCCTT CAAGTTCAAC AGAGATGACT TTAAGATGAA GGTGCCGGTC 
ATGATGCTGA GCTCAGTGCC AGAACTACAC GGCTACATTG ATAAGTCACA GCTGACCGAG 
GACCTCGGGG GGACCCTGGA CTACTGCCAT TCCAGGTGGC TGTGCCACCG CACGGCGATC 
GAAAGCTTCG CCCTGATGGT GAAGCAGACA GCTCAGATGC TGCAGGCCTT CGGGACCGAG 
CTGGCCGAAA CAGAACTGCC CAACGATGTC CAATCAACAA GCTCGGTGCT GAGCACACAC 
ACGGAGAAGA AGGACAAAGC AAAGGAGGAT CTGCAGCTGG CACGGAAAGA GGGGAACAGC 
ATCCTTGAGA GCCTCAGGGA GCCACTGGCT GAAAGCACTG CCCACAGTGT AAACCAAGAC 
CAGCTGGACA ATCAGGCCAC AGTGCAGAGG CTCCTGGCTC AGCTGAATGA AACGGAGGCC 
GCCTTTGACG AGTTCTGGGC AAAGCATCAG CAGAAGCTGG AACAGTGCCT GCAGCTGCGG 
CATTTTGAGC AGGGTTTCCG GGAGGTCAAA ACTACCTTGG ATACCATGTC CCACAAGATA 
GCCACCTTCA CTGACGTTGG TAACAGCCTG GCACATGTGC AACACCTGCT GAAGGACCTG 
GCCAGCTTCG AAGAGAAGTC TAGCGTGGCT GTAGACAGGG CCCGAGCCCT GTCACTGGAG 
GGTCAGCAGC TGATTGAGAA CAAGCACTAT GCTGTGGACT CCATCCACCC TAAGTGTGAG 
GAGCTCCAGC ACCTCTGCGA TCACTTCACC GCTGAGATCA GCAGGAGGAG AGGCTTGCTC 
AGCAAGTCCC TGGAGCTACA CAGCCTCTTG GAGACGTCCA TGAAGTGGTG CGATGAGGGC 
ATCTTCCTGC TGGCCTCGCA ACCTGTGGAC AAGTGTCAGT CCCAGGATGG TGCTGAGGCA 
GCCCTCCAGG AGATCGAGAA ATTTTTGGAG ACTGGTGCAG AAAATAAGAT TCAAGAACTC 
AATAAAATTT ACAAGGAATA TGAGTGCATC CTCAACCAGG ACCTACTGGA ACACGTGCAA 
AAGGTCTTTC AGAAGCAAGA AAGTATGCAA GAGATGTTCC ACCGCAGGCA GGCCAGCCTG 
AAGAAGCTGG CGGCCAAGCA GACACGGCCT GTGCAGCCCG TGGCCCCACG GCCAGAGGCA 
CTCACCAAAT CACCCTGCCC CTCCCCAGGC TCCTGGAGAG GATCTGAGAA CTCTAGCTCT 
GAGGGCAGTG CACTCCGTAG AGGGCCCTAC AGGAGAGCCA AGAGTGAAAT GAGTGAGACC 
CGGCAGGGCC GTGCCAGCTC CACAGGGGAC GAGGAGGAGA GCCTAGCCAT CCTGCGCAGG 
CATGTGATGA ATGAGCTCCT AGACACGGAA CGGGCCTACG TGGAGGAGCT GCTCTGTGTA 
CTAGAGGGCT ATGCTGCAGA GATGGACAAC CCCCTGATGG CACACCTCAT TTCAGCAGGC 
CTGCAAAACA AGAAGAACAT TCTGTTTGGA AACATGGAGG AGATTTATCA CTTTCATAAC 
AGGATATTCC TGCGGGAGCT AGAGAGCTGC ATAGACTGCC CGGAACTGGT GGGGAGGTGC 
TTTCTGGAGA GGATGGAGGA GTTCCAGATC TACGAGAAGT ACTGTCAGAA CAAGCCACGA 
TCTGAGAGCC TGTGGAGACA GTGCTCTGAC TGTCCCTTCT TCCAGGAGTG CCAGAAGAAG 
TTGGACCACA AACTGAGCCT TGATTCCTAC CTGCTGAAGC CAGTGCAGAG AATAACTAAG 
TACCAGTTGC TGCTTAAGGA AATGTTGAAA TACAGCAAGA ACTGTGAGGG GGCAGAGGAC 
CTACAGGAGG CACTGAGCTC CATCCTGGGC ATCCTCAAGG CAGTGAATGA CTCCATGCAC 
CTCATCGCCA TCACTGGCTA CGACGGTAAC CTGGGTGATC TGGGGAAGCT GCTGATGCAA 
GGTTCCTTCA GTGTATGGAC AGACCACAAG AAGGGCCACA CCAAGGTCAA GGAGCTGGCC 
AGATTCAAGC CCATGCAGCG GCACCTTTTC CTGCATGAGA AGGCTGTGCT GTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGGTATGAG AAGGCCCCTT CCTACAGCTA CAAGCAGTCA 
TTGAATATGA CTGCTGTTGG CATCACAGAG AATGTGAAGG GTGATGCCAA GAAGTTCGAG 
ATCTGGTACA ACGCAAGAGA GGAGGTTTAC ATCATCCAGG CACCAACCCC TGAGATTAAA 
GCAGCATGGG TGAGTGAGAT TCGGAAGGTG CTGACCAGCC AGCTGCAGGC CTGCCGGGAA 
GCCAGCCAGC ACAGGGCCCT GGAGCAGTCC CACAGCCTGC CTTTGCCCAC ACCATCCAAC 
ACCAGCCCCT CAAAAGGGAA CACAAGGAAT GTCAAGAAGT CAGAAGAAAG AAAAACGGAC 
CCCCTGAGCC TGGAAGGATA TGTGAGCTCA TCGTTGCCGA AGCCCCCTGA GAAAGGCAAA 
GACGATGCGG TCCCTAGTTC TACCTCTGAA AGCTCTGCGC TTTCCAGAAA GCGCTTCACG 
CTGCAGGGCT TGGCTAACCT CAAAGGTCAG AAAGCCTCTC CGACCAGTCC TGACAAAAAA 
GCTAAGCGGC ACGAAGTAAA GAGCGACCCG ACTCCCTTTG GTTTGCGAGG CTGGAGCAAA 
ACATCCCACT CCCTGGAAGC CCCTGAGGAG GACGGAGGTT GGTCCAGTGC TGAGGAGCTT 
GTCAATTCAT CTGATGCAGA GGAAGATGGA GTGGGCCCCA AGAAGCTGGT TCCTGGTAAA 
TACACAGTGG TGATGGACGA TGAGAAAGGG GGCCTTGACA CCCTTGCTGT GAGGAGCGGG 
GACATGGTAG AGGTGGTGGA AGAGGGCGCA GAGGGGCTCT GGTATGTCCG GGACCTGACC 
AGCAGCAAGG AGGGATGGGT GCCAGCCAGC AGCCTTTCCA CCCTCCTGGG CAAATCCAGT 
TCAGCCCAGT GTCTGAGCAG CTCAGACTCC AGCCCAGGGT CAGCCGTGCT GAGCAACTCC 
TCCAGCTGCA GCGAGGGCTG CCCCACTCCC TTCTCCGACC TCCAGGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846636.1
CDS	123..3653
Translation	MRPHGERALGCCGLCTCPAAAGDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_008848414.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848414.2

ORF Insert Sequence:

ATGAGACCTC ATGGAGAGCG TGCGCTGGGC TGCTGCGGCC TCTGCACCTG CCCGGCAGCA 
GCAGGAGATG AAATCATGCA CCAGGACATC GTCCCACTCT GTGCTGCGGA CATCCAGGAG 
CAGCTGAAGA AGCGCTTTGC TTACCTGTCT GGTGGGCGGG GGCAGGACGG AAGCCCAGTT 
ATCACCTTTC CAGACTACCC AGCCTTCAGC GAGATCCCGG ACAAGGAGTT CCAGAATGTC 
ATGACCTATC TCACCAGCAT CCCCAGTTTG CAAGATGCTG GCATTGGATT CATTCTGGTC 
ATAGACCGAA GGCAGGACAA ATGGACCTCC GTGAAGGCGT CGGTCCTGCG AATAGCCGCA 
TCTTTCCCAG CAAACCTGCA GCTCGTCCTT GTGCTTCGCC CAACGGGCTT TTTCCAGAGG 
ACTCTCTCTG ACCTCGCCTT CAAGTTCAAC AGAGATGACT TTAAGATGAA GGTGCCGGTC 
ATGATGCTGA GCTCAGTGCC AGAACTACAC GGCTACATTG ATAAGTCACA GCTGACCGAG 
GACCTCGGGG GGACCCTGGA CTACTGCCAT TCCAGGTGGC TGTGCCACCG CACGGCGATC 
GAAAGCTTCG CCCTGATGGT GAAGCAGACA GCTCAGATGC TGCAGGCCTT CGGGACCGAG 
CTGGCCGAAA CAGAACTGCC CAACGATGTC CAATCAACAA GCTCGGTGCT GAGCACACAC 
ACGGAGAAGA AGGACAAAGC AAAGGAGGAT CTGCAGCTGG CACGGAAAGA GGGGAACAGC 
ATCCTTGAGA GCCTCAGGGA GCCACTGGCT GAAAGCACTG CCCACAGTGT AAACCAAGAC 
CAGCTGGACA ATCAGGCCAC AGTGCAGAGG CTCCTGGCTC AGCTGAATGA AACGGAGGCC 
GCCTTTGACG AGTTCTGGGC AAAGCATCAG CAGAAGCTGG AACAGTGCCT GCAGCTGCGG 
CATTTTGAGC AGGGTTTCCG GGAGGTCAAA ACTACCTTGG ATACCATGTC CCACAAGATA 
GCCACCTTCA CTGACGTTGG TAACAGCCTG GCACATGTGC AACACCTGCT GAAGGACCTG 
GCCAGCTTCG AAGAGAAGTC TAGCGTGGCT GTAGACAGGG CCCGAGCCCT GTCACTGGAG 
GGTCAGCAGC TGATTGAGAA CAAGCACTAT GCTGTGGACT CCATCCACCC TAAGTGTGAG 
GAGCTCCAGC ACCTCTGCGA TCACTTCACC GCTGAGATCA GCAGGAGGAG AGGCTTGCTC 
AGCAAGTCCC TGGAGCTACA CAGCCTCTTG GAGACGTCCA TGAAGTGGTG CGATGAGGGC 
ATCTTCCTGC TGGCCTCGCA ACCTGTGGAC AAGTGTCAGT CCCAGGATGG TGCTGAGGCA 
GCCCTCCAGG AGATCGAGAA ATTTTTGGAG ACTGGTGCAG AAAATAAGAT TCAAGAACTC 
AATAAAATTT ACAAGGAATA TGAGTGCATC CTCAACCAGG ACCTACTGGA ACACGTGCAA 
AAGGTCTTTC AGAAGCAAGA AAGTATGCAA GAGATGTTCC ACCGCAGGCA GGCCAGCCTG 
AAGAAGCTGG CGGCCAAGCA GACACGGCCT GTGCAGCCCG TGGCCCCACG GCCAGAGGCA 
CTCACCAAAT CACCCTGCCC CTCCCCAGGC TCCTGGAGAG GATCTGAGAA CTCTAGCTCT 
GAGGGCAGTG CACTCCGTAG AGGGCCCTAC AGGAGAGCCA AGAGTGAAAT GAGTGAGACC 
CGGCAGGGCC GTGCCAGCTC CACAGGGGAC GAGGAGGAGA GCCTAGCCAT CCTGCGCAGG 
CATGTGATGA ATGAGCTCCT AGACACGGAA CGGGCCTACG TGGAGGAGCT GCTCTGTGTA 
CTAGAGGGCT ATGCTGCAGA GATGGACAAC CCCCTGATGG CACACCTCAT TTCAGCAGGC 
CTGCAAAACA AGAAGAACAT TCTGTTTGGA AACATGGAGG AGATTTATCA CTTTCATAAC 
AGGATATTCC TGCGGGAGCT AGAGAGCTGC ATAGACTGCC CGGAACTGGT GGGGAGGTGC 
TTTCTGGAGA GGATGGAGGA GTTCCAGATC TACGAGAAGT ACTGTCAGAA CAAGCCACGA 
TCTGAGAGCC TGTGGAGACA GTGCTCTGAC TGTCCCTTCT TCCAGGAGTG CCAGAAGAAG 
TTGGACCACA AACTGAGCCT TGATTCCTAC CTGCTGAAGC CAGTGCAGAG AATAACTAAG 
TACCAGTTGC TGCTTAAGGA AATGTTGAAA TACAGCAAGA ACTGTGAGGG GGCAGAGGAC 
CTACAGGAGG CACTGAGCTC CATCCTGGGC ATCCTCAAGG CAGTGAATGA CTCCATGCAC 
CTCATCGCCA TCACTGGCTA CGACGGTAAC CTGGGTGATC TGGGGAAGCT GCTGATGCAA 
GGTTCCTTCA GTGTATGGAC AGACCACAAG AAGGGCCACA CCAAGGTCAA GGAGCTGGCC 
AGATTCAAGC CCATGCAGCG GCACCTTTTC CTGCATGAGA AGGCTGTGCT GTTCTGCAAG 
AAGAGGGAGG AGAATGGGGA GGGGTATGAG AAGGCCCCTT CCTACAGCTA CAAGCAGTCA 
TTGAATATGA CTGCTGTTGG CATCACAGAG AATGTGAAGG GTGATGCCAA GAAGTTCGAG 
ATCTGGTACA ACGCAAGAGA GGAGGTTTAC ATCATCCAGG CACCAACCCC TGAGATTAAA 
GCAGCATGGG TGAGTGAGAT TCGGAAGGTG CTGACCAGCC AGCTGCAGGC CTGCCGGGAA 
GCCAGCCAGC ACAGGGCCCT GGAGCAGTCC CACAGCCTGC CTTTGCCCAC ACCATCCAAC 
ACCAGCCCCT CAAAAGGGAA CACAAGGAAT GTCAAGAAGT CAGAAGAAAG AAAAACGGAC 
CCCCTGAGCC TGGAAGGATA TGTGAGCTCA TCGTTGCCGA AGCCCCCTGA GAAAGGCAAA 
GACGATGCGG TCCCTAGTTC TACCTCTGAA AGCTCTGCGC TTTCCAGAAA GCGCTTCACG 
CTGCAGGGCT TGGCTAACCT CAAAGGTCAG AAAGCCTCTC CGACCAGTCC TGACAAAAAA 
GCTAAGCGGC ACGAAGTAAA GAGCGACCCG ACTCCCTTTG GTTTGCGAGG CTGGAGCAAA 
ACATCCCACT CCCTGGAAGC CCCTGAGGAG GACGGAGGTT GGTCCAGTGC TGAGGAGCTT 
GTCAATTCAT CTGATGCAGA GGAAGATGGA GTGGGCCCCA AGAAGCTGGT TCCTGGTAAA 
TACACAGTGG TGATGGACGA TGAGAAAGGG GGCCTTGACA CCCTTGCTGT GAGGAGCGGG 
GACATGGTAG AGGTGGTGGA AGAGGGCGCA GAGGGGCTCT GGTATGTCCG GGACCTGACC 
AGCAGCAAGG AGGGATGGGT GCCAGCCAGC AGCCTTTCCA CCCTCCTGGG CAAATCCAGT 
TCAGCCCAGT GTCTGAGCAG CTCAGACTCC AGCCCAGGGT CAGCCGTGCT GAGCAACTCC 
TCCAGCTGCA GCGAGGGCTG CCCCACTCCC TTCTCCGACC TCCAGGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848416.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846638.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X8, mRNA.

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CloneID	ONa31079
Clone ID Related Accession (Same CDS sequence)	XM_008848416.1 , XM_008848416.2
Accession Version	XM_008848416.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG GCTGGAGCAA AACATCCCAC 
TCCCTGGAAG CCCCTGAGGA GGACGGAGGT TGGTCCAGTG CTGAGGAGCT TGTCAATTCA 
TCTGATGCAG AGGAAGATGG AGTGGGCCCC AAGAAGCTGG TTCCTGGTAA ATACACAGTG 
GTGATGGACG ATGAGAAAGG GGGCCTTGAC ACCCTTGCTG TGAGGAGCGG GGACATGGTA 
GAGGTGGTGG AAGAGGGCGC AGAGGGGCTC TGGTATGTCC GGGACCTGAC CAGCAGCAAG 
GAGGGATGGG TGCCAGCCAG CAGCCTTTCC ACCCTCCTGG GCAAATCCAG TTCAGCCCAG 
TGTCTGAGCA GCTCAGACTC CAGCCCAGGG TCAGCCGTGC TGAGCAACTC CTCCAGCTGC 
AGCGAGGGCT GCCCCACTCC CTTCTCCGAC CTCCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846638.1
CDS	29..3490
Translation	MSDCWRFILCKDRGRDSPSPQEHNGVPKEEADHQVDVSDGIKLVPDKAEAMVTAPDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_008848416.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848416.1

ORF Insert Sequence:

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG GCTGGAGCAA AACATCCCAC 
TCCCTGGAAG CCCCTGAGGA GGACGGAGGT TGGTCCAGTG CTGAGGAGCT TGTCAATTCA 
TCTGATGCAG AGGAAGATGG AGTGGGCCCC AAGAAGCTGG TTCCTGGTAA ATACACAGTG 
GTGATGGACG ATGAGAAAGG GGGCCTTGAC ACCCTTGCTG TGAGGAGCGG GGACATGGTA 
GAGGTGGTGG AAGAGGGCGC AGAGGGGCTC TGGTATGTCC GGGACCTGAC CAGCAGCAAG 
GAGGGATGGG TGCCAGCCAG CAGCCTTTCC ACCCTCCTGG GCAAATCCAG TTCAGCCCAG 
TGTCTGAGCA GCTCAGACTC CAGCCCAGGG TCAGCCGTGC TGAGCAACTC CTCCAGCTGC 
AGCGAGGGCT GCCCCACTCC CTTCTCCGAC CTCCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848416.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846638.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ONa31079
Clone ID Related Accession (Same CDS sequence)	XM_008848416.1 , XM_008848416.2
Accession Version	XM_008848416.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008848416.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG GCTGGAGCAA AACATCCCAC 
TCCCTGGAAG CCCCTGAGGA GGACGGAGGT TGGTCCAGTG CTGAGGAGCT TGTCAATTCA 
TCTGATGCAG AGGAAGATGG AGTGGGCCCC AAGAAGCTGG TTCCTGGTAA ATACACAGTG 
GTGATGGACG ATGAGAAAGG GGGCCTTGAC ACCCTTGCTG TGAGGAGCGG GGACATGGTA 
GAGGTGGTGG AAGAGGGCGC AGAGGGGCTC TGGTATGTCC GGGACCTGAC CAGCAGCAAG 
GAGGGATGGG TGCCAGCCAG CAGCCTTTCC ACCCTCCTGG GCAAATCCAG TTCAGCCCAG 
TGTCTGAGCA GCTCAGACTC CAGCCCAGGG TCAGCCGTGC TGAGCAACTC CTCCAGCTGC 
AGCGAGGGCT GCCCCACTCC CTTCTCCGAC CTCCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846638.1
CDS	163..3624
Translation	MSDCWRFILCKDRGRDSPSPQEHNGVPKEEADHQVDVSDGIKLVPDKAEAMVTAPDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_008848416.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848416.2

ORF Insert Sequence:

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG GCTGGAGCAA AACATCCCAC 
TCCCTGGAAG CCCCTGAGGA GGACGGAGGT TGGTCCAGTG CTGAGGAGCT TGTCAATTCA 
TCTGATGCAG AGGAAGATGG AGTGGGCCCC AAGAAGCTGG TTCCTGGTAA ATACACAGTG 
GTGATGGACG ATGAGAAAGG GGGCCTTGAC ACCCTTGCTG TGAGGAGCGG GGACATGGTA 
GAGGTGGTGG AAGAGGGCGC AGAGGGGCTC TGGTATGTCC GGGACCTGAC CAGCAGCAAG 
GAGGGATGGG TGCCAGCCAG CAGCCTTTCC ACCCTCCTGG GCAAATCCAG TTCAGCCCAG 
TGTCTGAGCA GCTCAGACTC CAGCCCAGGG TCAGCCGTGC TGAGCAACTC CTCCAGCTGC 
AGCGAGGGCT GCCCCACTCC CTTCTCCGAC CTCCAGGGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848417.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846639.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X9, mRNA.

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CloneID	ONa31080
Clone ID Related Accession (Same CDS sequence)	XM_008848417.1 , XM_008848417.2
Accession Version	XM_008848417.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG GCTGGAGCAA AACATCCCAC 
TCCCTGGAAG CCCCTGAGGA GGACGGAGGT TGGTCCAGTG CTGAGGAGCT TGTCAATTCA 
TCTGATGCAG AGGAAGATGG AGTGGGCCCC AAGAAGCTGG TTCCTGGTAA ATACACAGTG 
GTGATGGACG ATGAGAAAGG GGGCCTTGAC ACCCTTGCTG TGAGGAGCGG GGACATGGTA 
GAGGTGGTGG AAGAGGGCGC AGAGGGGCTC TGGTATGTCC GGGACCTGAC CAGCAGCAAG 
GAGGGATGGG TGCCAGCCAG CAGCCTTTCC ACCCTCCTGG GCAAATCCAG TTCAGCCCAG 
TGTCTGAGCA GCTCAGGTGT GCAGAGGAAA GAGCCAGAGC CGAAGCCTGT CTCCACTGGT 
TTCCCGCAGA CTCCAGCCCA GGGTCAGCCG TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846639.1
CDS	29..3484
Translation	MSDCWRFILCKDRGRDSPSPQEHNGVPKEEADHQVDVSDGIKLVPDKAEAMVTAPDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGVQRKEPEPKPVSTGFPQTPAQGQPC

Target ORF information:

RefSeq Version	XM_008848417.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848417.1

ORF Insert Sequence:

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG GCTGGAGCAA AACATCCCAC 
TCCCTGGAAG CCCCTGAGGA GGACGGAGGT TGGTCCAGTG CTGAGGAGCT TGTCAATTCA 
TCTGATGCAG AGGAAGATGG AGTGGGCCCC AAGAAGCTGG TTCCTGGTAA ATACACAGTG 
GTGATGGACG ATGAGAAAGG GGGCCTTGAC ACCCTTGCTG TGAGGAGCGG GGACATGGTA 
GAGGTGGTGG AAGAGGGCGC AGAGGGGCTC TGGTATGTCC GGGACCTGAC CAGCAGCAAG 
GAGGGATGGG TGCCAGCCAG CAGCCTTTCC ACCCTCCTGG GCAAATCCAG TTCAGCCCAG 
TGTCTGAGCA GCTCAGGTGT GCAGAGGAAA GAGCCAGAGC CGAAGCCTGT CTCCACTGGT 
TTCCCGCAGA CTCCAGCCCA GGGTCAGCCG TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848417.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846639.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X10, mRNA.

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CloneID	ONa31080
Clone ID Related Accession (Same CDS sequence)	XM_008848417.1 , XM_008848417.2
Accession Version	XM_008848417.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008848417.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG GCTGGAGCAA AACATCCCAC 
TCCCTGGAAG CCCCTGAGGA GGACGGAGGT TGGTCCAGTG CTGAGGAGCT TGTCAATTCA 
TCTGATGCAG AGGAAGATGG AGTGGGCCCC AAGAAGCTGG TTCCTGGTAA ATACACAGTG 
GTGATGGACG ATGAGAAAGG GGGCCTTGAC ACCCTTGCTG TGAGGAGCGG GGACATGGTA 
GAGGTGGTGG AAGAGGGCGC AGAGGGGCTC TGGTATGTCC GGGACCTGAC CAGCAGCAAG 
GAGGGATGGG TGCCAGCCAG CAGCCTTTCC ACCCTCCTGG GCAAATCCAG TTCAGCCCAG 
TGTCTGAGCA GCTCAGGTGT GCAGAGGAAA GAGCCAGAGC CGAAGCCTGT CTCCACTGGT 
TTCCCGCAGA CTCCAGCCCA GGGTCAGCCG TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846639.1
CDS	163..3618
Translation	MSDCWRFILCKDRGRDSPSPQEHNGVPKEEADHQVDVSDGIKLVPDKAEAMVTAPDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSGVQRKEPEPKPVSTGFPQTPAQGQPC

Target ORF information:

RefSeq Version	XM_008848417.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848417.2

ORF Insert Sequence:

ATGTCCGACT GCTGGAGATT TATCTTGTGC AAGGACCGTG GAAGAGACAG CCCCTCACCA 
CAGGAGCACA ATGGGGTCCC GAAGGAGGAG GCTGATCACC AAGTGGATGT GTCTGACGGT 
ATCAAGCTTG TTCCAGACAA AGCAGAGGCA ATGGTCACAG CCCCAGATGA AATCATGCAC 
CAGGACATCG TCCCACTCTG TGCTGCGGAC ATCCAGGAGC AGCTGAAGAA GCGCTTTGCT 
TACCTGTCTG GTGGGCGGGG GCAGGACGGA AGCCCAGTTA TCACCTTTCC AGACTACCCA 
GCCTTCAGCG AGATCCCGGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGTTTGC AAGATGCTGG CATTGGATTC ATTCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCCG TGAAGGCGTC GGTCCTGCGA ATAGCCGCAT CTTTCCCAGC AAACCTGCAG 
CTCGTCCTTG TGCTTCGCCC AACGGGCTTT TTCCAGAGGA CTCTCTCTGA CCTCGCCTTC 
AAGTTCAACA GAGATGACTT TAAGATGAAG GTGCCGGTCA TGATGCTGAG CTCAGTGCCA 
GAACTACACG GCTACATTGA TAAGTCACAG CTGACCGAGG ACCTCGGGGG GACCCTGGAC 
TACTGCCATT CCAGGTGGCT GTGCCACCGC ACGGCGATCG AAAGCTTCGC CCTGATGGTG 
AAGCAGACAG CTCAGATGCT GCAGGCCTTC GGGACCGAGC TGGCCGAAAC AGAACTGCCC 
AACGATGTCC AATCAACAAG CTCGGTGCTG AGCACACACA CGGAGAAGAA GGACAAAGCA 
AAGGAGGATC TGCAGCTGGC ACGGAAAGAG GGGAACAGCA TCCTTGAGAG CCTCAGGGAG 
CCACTGGCTG AAAGCACTGC CCACAGTGTA AACCAAGACC AGCTGGACAA TCAGGCCACA 
GTGCAGAGGC TCCTGGCTCA GCTGAATGAA ACGGAGGCCG CCTTTGACGA GTTCTGGGCA 
AAGCATCAGC AGAAGCTGGA ACAGTGCCTG CAGCTGCGGC ATTTTGAGCA GGGTTTCCGG 
GAGGTCAAAA CTACCTTGGA TACCATGTCC CACAAGATAG CCACCTTCAC TGACGTTGGT 
AACAGCCTGG CACATGTGCA ACACCTGCTG AAGGACCTGG CCAGCTTCGA AGAGAAGTCT 
AGCGTGGCTG TAGACAGGGC CCGAGCCCTG TCACTGGAGG GTCAGCAGCT GATTGAGAAC 
AAGCACTATG CTGTGGACTC CATCCACCCT AAGTGTGAGG AGCTCCAGCA CCTCTGCGAT 
CACTTCACCG CTGAGATCAG CAGGAGGAGA GGCTTGCTCA GCAAGTCCCT GGAGCTACAC 
AGCCTCTTGG AGACGTCCAT GAAGTGGTGC GATGAGGGCA TCTTCCTGCT GGCCTCGCAA 
CCTGTGGACA AGTGTCAGTC CCAGGATGGT GCTGAGGCAG CCCTCCAGGA GATCGAGAAA 
TTTTTGGAGA CTGGTGCAGA AAATAAGATT CAAGAACTCA ATAAAATTTA CAAGGAATAT 
GAGTGCATCC TCAACCAGGA CCTACTGGAA CACGTGCAAA AGGTCTTTCA GAAGCAAGAA 
AGTATGCAAG AGATGTTCCA CCGCAGGCAG GCCAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACACGGCCTG TGCAGCCCGT GGCCCCACGG CCAGAGGCAC TCACCAAATC ACCCTGCCCC 
TCCCCAGGCT CCTGGAGAGG ATCTGAGAAC TCTAGCTCTG AGGGCAGTGC ACTCCGTAGA 
GGGCCCTACA GGAGAGCCAA GAGTGAAATG AGTGAGACCC GGCAGGGCCG TGCCAGCTCC 
ACAGGGGACG AGGAGGAGAG CCTAGCCATC CTGCGCAGGC ATGTGATGAA TGAGCTCCTA 
GACACGGAAC GGGCCTACGT GGAGGAGCTG CTCTGTGTAC TAGAGGGCTA TGCTGCAGAG 
ATGGACAACC CCCTGATGGC ACACCTCATT TCAGCAGGCC TGCAAAACAA GAAGAACATT 
CTGTTTGGAA ACATGGAGGA GATTTATCAC TTTCATAACA GGATATTCCT GCGGGAGCTA 
GAGAGCTGCA TAGACTGCCC GGAACTGGTG GGGAGGTGCT TTCTGGAGAG GATGGAGGAG 
TTCCAGATCT ACGAGAAGTA CTGTCAGAAC AAGCCACGAT CTGAGAGCCT GTGGAGACAG 
TGCTCTGACT GTCCCTTCTT CCAGGAGTGC CAGAAGAAGT TGGACCACAA ACTGAGCCTT 
GATTCCTACC TGCTGAAGCC AGTGCAGAGA ATAACTAAGT ACCAGTTGCT GCTTAAGGAA 
ATGTTGAAAT ACAGCAAGAA CTGTGAGGGG GCAGAGGACC TACAGGAGGC ACTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC AGTGAATGAC TCCATGCACC TCATCGCCAT CACTGGCTAC 
GACGGTAACC TGGGTGATCT GGGGAAGCTG CTGATGCAAG GTTCCTTCAG TGTATGGACA 
GACCACAAGA AGGGCCACAC CAAGGTCAAG GAGCTGGCCA GATTCAAGCC CATGCAGCGG 
CACCTTTTCC TGCATGAGAA GGCTGTGCTG TTCTGCAAGA AGAGGGAGGA GAATGGGGAG 
GGGTATGAGA AGGCCCCTTC CTACAGCTAC AAGCAGTCAT TGAATATGAC TGCTGTTGGC 
ATCACAGAGA ATGTGAAGGG TGATGCCAAG AAGTTCGAGA TCTGGTACAA CGCAAGAGAG 
GAGGTTTACA TCATCCAGGC ACCAACCCCT GAGATTAAAG CAGCATGGGT GAGTGAGATT 
CGGAAGGTGC TGACCAGCCA GCTGCAGGCC TGCCGGGAAG CCAGCCAGCA CAGGGCCCTG 
GAGCAGTCCC ACAGCCTGCC TTTGCCCACA CCATCCAACA CCAGCCCCTC AAAAGGGAAC 
ACAAGGAATG TCAAGAAGTC AGAAGAAAGA AAAACGGACC CCCTGAGCCT GGAAGGATAT 
GTGAGCTCAT CGTTGCCGAA GCCCCCTGAG AAAGGCAAAG GCTGGAGCAA AACATCCCAC 
TCCCTGGAAG CCCCTGAGGA GGACGGAGGT TGGTCCAGTG CTGAGGAGCT TGTCAATTCA 
TCTGATGCAG AGGAAGATGG AGTGGGCCCC AAGAAGCTGG TTCCTGGTAA ATACACAGTG 
GTGATGGACG ATGAGAAAGG GGGCCTTGAC ACCCTTGCTG TGAGGAGCGG GGACATGGTA 
GAGGTGGTGG AAGAGGGCGC AGAGGGGCTC TGGTATGTCC GGGACCTGAC CAGCAGCAAG 
GAGGGATGGG TGCCAGCCAG CAGCCTTTCC ACCCTCCTGG GCAAATCCAG TTCAGCCCAG 
TGTCTGAGCA GCTCAGGTGT GCAGAGGAAA GAGCCAGAGC CGAAGCCTGT CTCCACTGGT 
TTCCCGCAGA CTCCAGCCCA GGGTCAGCCG TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2l ( XM_008848418.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846640.1 Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X11, mRNA.

Price & Availability↑

CloneID	ONa31081
Clone ID Related Accession (Same CDS sequence)	XM_008848418.2
Accession Version	XM_008848418.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	guanine nucleotide exchange factor DBS isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008848418.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCTCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCACT CTGTGCTGCG 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGGCAGGAC 
GGAAGCCCAG TTATCACCTT TCCAGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATTCTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CCGTGAAGGC GTCGGTCCTG 
CGAATAGCCG CATCTTTCCC AGCAAACCTG CAGCTCGTCC TTGTGCTTCG CCCAACGGGC 
TTTTTCCAGA GGACTCTCTC TGACCTCGCC TTCAAGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATGATGCT GAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCA 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ATTCCAGGTG GCTGTGCCAC 
CGCACGGCGA TCGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCTCAGAT GCTGCAGGCC 
TTCGGGACCG AGCTGGCCGA AACAGAACTG CCCAACGATG TCCAATCAAC AAGCTCGGTG 
CTGAGCACAC ACACGGAGAA GAAGGACAAA GCAAAGGAGG ATCTGCAGCT GGCACGGAAA 
GAGGGGAACA GCATCCTTGA GAGCCTCAGG GAGCCACTGG CTGAAAGCAC TGCCCACAGT 
GTAAACCAAG ACCAGCTGGA CAATCAGGCC ACAGTGCAGA GGCTCCTGGC TCAGCTGAAT 
GAAACGGAGG CCGCCTTTGA CGAGTTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAGGGTTTC CGGGAGGTCA AAACTACCTT GGATACCATG 
TCCCACAAGA TAGCCACCTT CACTGACGTT GGTAACAGCC TGGCACATGT GCAACACCTG 
CTGAAGGACC TGGCCAGCTT CGAAGAGAAG TCTAGCGTGG CTGTAGACAG GGCCCGAGCC 
CTGTCACTGG AGGGTCAGCA GCTGATTGAG AACAAGCACT ATGCTGTGGA CTCCATCCAC 
CCTAAGTGTG AGGAGCTCCA GCACCTCTGC GATCACTTCA CCGCTGAGAT CAGCAGGAGG 
AGAGGCTTGC TCAGCAAGTC CCTGGAGCTA CACAGCCTCT TGGAGACGTC CATGAAGTGG 
TGCGATGAGG GCATCTTCCT GCTGGCCTCG CAACCTGTGG ACAAGTGTCA GTCCCAGGAT 
GGTGCTGAGG CAGCCCTCCA GGAGATCGAG AAATTTTTGG AGACTGGTGC AGAAAATAAG 
ATTCAAGAAC TCAATAAAAT TTACAAGGAA TATGAGTGCA TCCTCAACCA GGACCTACTG 
GAACACGTGC AAAAGGTCTT TCAGAAGCAA GAAAGTATGC AAGAGATGTT CCACCGCAGG 
CAGGCCAGCC TGAAGAAGCT GGCGGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCA 
CGGCCAGAGG CACTCACCAA ATCACCCTGC CCCTCCCCAG GCTCCTGGAG AGGATCTGAG 
AACTCTAGCT CTGAGGGCAG TGCACTCCGT AGAGGGCCCT ACAGGAGAGC CAAGAGTGAA 
ATGAGTGAGA CCCGGCAGGG CCGTGCCAGC TCCACAGGGG ACGAGGAGGA GAGCCTAGCC 
ATCCTGCGCA GGCATGTGAT GAATGAGCTC CTAGACACGG AACGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TACTAGAGGG CTATGCTGCA GAGATGGACA ACCCCCTGAT GGCACACCTC 
ATTTCAGCAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAGATTTAT 
CACTTTCATA ACAGGATATT CCTGCGGGAG CTAGAGAGCT GCATAGACTG CCCGGAACTG 
GTGGGGAGGT GCTTTCTGGA GAGGATGGAG GAGTTCCAGA TCTACGAGAA GTACTGTCAG 
AACAAGCCAC GATCTGAGAG CCTGTGGAGA CAGTGCTCTG ACTGTCCCTT CTTCCAGGAG 
TGCCAGAAGA AGTTGGACCA CAAACTGAGC CTTGATTCCT ACCTGCTGAA GCCAGTGCAG 
AGAATAACTA AGTACCAGTT GCTGCTTAAG GAAATGTTGA AATACAGCAA GAACTGTGAG 
GGGGCAGAGG ACCTACAGGA GGCACTGAGC TCCATCCTGG GCATCCTCAA GGCAGTGAAT 
GACTCCATGC ACCTCATCGC CATCACTGGC TACGACGGTA ACCTGGGTGA TCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGTGTATGG ACAGACCACA AGAAGGGCCA CACCAAGGTC 
AAGGAGCTGG CCAGATTCAA GCCCATGCAG CGGCACCTTT TCCTGCATGA GAAGGCTGTG 
CTGTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGGTATG AGAAGGCCCC TTCCTACAGC 
TACAAGCAGT CATTGAATAT GACTGCTGTT GGCATCACAG AGAATGTGAA GGGTGATGCC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGA GAGGAGGTTT ACATCATCCA GGCACCAACC 
CCTGAGATTA AAGCAGCATG GGTGAGTGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCCTGCCGGG AAGCCAGCCA GCACAGGGCC CTGGAGCAGT CCCACAGCCT GCCTTTGCCC 
ACACCATCCA ACACCAGCCC CTCAAAAGGG AACACAAGGA ATGTCAAGAA GTCAGAAGAA 
AGAAAAACGG ACCCCCTGAG CCTGGAAGGA TATGTGAGCT CATCGTTGCC GAAGCCCCCT 
GAGAAAGGCA AAGGCTGGAG CAAAACATCC CACTCCCTGG AAGCCCCTGA GGAGGACGGA 
GGTTGGTCCA GTGCTGAGGA GCTTGTCAAT TCATCTGATG CAGAGGAAGA TGGAGTGGGC 
CCCAAGAAGC TGGTTCCTGG TAAATACACA GTGGTGATGG ACGATGAGAA AGGGGGCCTT 
GACACCCTTG CTGTGAGGAG CGGGGACATG GTAGAGGTGG TGGAAGAGGG CGCAGAGGGG 
CTCTGGTATG TCCGGGACCT GACCAGCAGC AAGGAGGGAT GGGTGCCAGC CAGCAGCCTT 
TCCACCCTCC TGGGCAAATC CAGTTCAGCC CAGTGTCTGA GCAGCTCAGA CTCCAGCCCA 
GGGTCAGCCG TGCTGAGCAA CTCCTCCAGC TGCAGCGAGG GCTGCCCCAC TCCCTTCTCC 
GACCTCCAGG GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846640.1
CDS	10..3384
Translation	MRFWLRNEEMALEEMVQRLNAVSKNTDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDLAFKFNRDDFKMKVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSSVLSTHTEKKDKAKEDLQLARKEGNSILESLREPLAESTAHSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTTLDTMSHKIATFTDVGNSLAHVQHLLKDLASFEEKSSVAVDRARALSLEGQQLIENKHYAVDSIHPKCEELQHLCDHFTAEISRRRGLLSKSLELHSLLETSMKWCDEGIFLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESMQEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPCPSPGSWRGSENSSSEGSALRRGPYRRAKSEMSETRQGRASSTGDEEESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISAGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDAKKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKVLTSQLQACREASQHRALEQSHSLPLPTPSNTSPSKGNTRNVKKSEERKTDPLSLEGYVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELVNSSDAEEDGVGPKKLVPGKYTVVMDDEKGGLDTLAVRSGDMVEVVEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGCPTPFSDLQG

Target ORF information:

RefSeq Version	XM_008848418.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili MCF.2 cell line derived transforming sequence like (Mcf2l), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848418.2

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCTCTAGAAG AAATGGTGCA GAGATTAAAC 
GCGGTTTCCA AGAACACTGA TGAAATCATG CACCAGGACA TCGTCCCACT CTGTGCTGCG 
GACATCCAGG AGCAGCTGAA GAAGCGCTTT GCTTACCTGT CTGGTGGGCG GGGGCAGGAC 
GGAAGCCCAG TTATCACCTT TCCAGACTAC CCAGCCTTCA GCGAGATCCC GGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGTT TGCAAGATGC TGGCATTGGA 
TTCATTCTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CCGTGAAGGC GTCGGTCCTG 
CGAATAGCCG CATCTTTCCC AGCAAACCTG CAGCTCGTCC TTGTGCTTCG CCCAACGGGC 
TTTTTCCAGA GGACTCTCTC TGACCTCGCC TTCAAGTTCA ACAGAGATGA CTTTAAGATG 
AAGGTGCCGG TCATGATGCT GAGCTCAGTG CCAGAACTAC ACGGCTACAT TGATAAGTCA 
CAGCTGACCG AGGACCTCGG GGGGACCCTG GACTACTGCC ATTCCAGGTG GCTGTGCCAC 
CGCACGGCGA TCGAAAGCTT CGCCCTGATG GTGAAGCAGA CAGCTCAGAT GCTGCAGGCC 
TTCGGGACCG AGCTGGCCGA AACAGAACTG CCCAACGATG TCCAATCAAC AAGCTCGGTG 
CTGAGCACAC ACACGGAGAA GAAGGACAAA GCAAAGGAGG ATCTGCAGCT GGCACGGAAA 
GAGGGGAACA GCATCCTTGA GAGCCTCAGG GAGCCACTGG CTGAAAGCAC TGCCCACAGT 
GTAAACCAAG ACCAGCTGGA CAATCAGGCC ACAGTGCAGA GGCTCCTGGC TCAGCTGAAT 
GAAACGGAGG CCGCCTTTGA CGAGTTCTGG GCAAAGCATC AGCAGAAGCT GGAACAGTGC 
CTGCAGCTGC GGCATTTTGA GCAGGGTTTC CGGGAGGTCA AAACTACCTT GGATACCATG 
TCCCACAAGA TAGCCACCTT CACTGACGTT GGTAACAGCC TGGCACATGT GCAACACCTG 
CTGAAGGACC TGGCCAGCTT CGAAGAGAAG TCTAGCGTGG CTGTAGACAG GGCCCGAGCC 
CTGTCACTGG AGGGTCAGCA GCTGATTGAG AACAAGCACT ATGCTGTGGA CTCCATCCAC 
CCTAAGTGTG AGGAGCTCCA GCACCTCTGC GATCACTTCA CCGCTGAGAT CAGCAGGAGG 
AGAGGCTTGC TCAGCAAGTC CCTGGAGCTA CACAGCCTCT TGGAGACGTC CATGAAGTGG 
TGCGATGAGG GCATCTTCCT GCTGGCCTCG CAACCTGTGG ACAAGTGTCA GTCCCAGGAT 
GGTGCTGAGG CAGCCCTCCA GGAGATCGAG AAATTTTTGG AGACTGGTGC AGAAAATAAG 
ATTCAAGAAC TCAATAAAAT TTACAAGGAA TATGAGTGCA TCCTCAACCA GGACCTACTG 
GAACACGTGC AAAAGGTCTT TCAGAAGCAA GAAAGTATGC AAGAGATGTT CCACCGCAGG 
CAGGCCAGCC TGAAGAAGCT GGCGGCCAAG CAGACACGGC CTGTGCAGCC CGTGGCCCCA 
CGGCCAGAGG CACTCACCAA ATCACCCTGC CCCTCCCCAG GCTCCTGGAG AGGATCTGAG 
AACTCTAGCT CTGAGGGCAG TGCACTCCGT AGAGGGCCCT ACAGGAGAGC CAAGAGTGAA 
ATGAGTGAGA CCCGGCAGGG CCGTGCCAGC TCCACAGGGG ACGAGGAGGA GAGCCTAGCC 
ATCCTGCGCA GGCATGTGAT GAATGAGCTC CTAGACACGG AACGGGCCTA CGTGGAGGAG 
CTGCTCTGTG TACTAGAGGG CTATGCTGCA GAGATGGACA ACCCCCTGAT GGCACACCTC 
ATTTCAGCAG GCCTGCAAAA CAAGAAGAAC ATTCTGTTTG GAAACATGGA GGAGATTTAT 
CACTTTCATA ACAGGATATT CCTGCGGGAG CTAGAGAGCT GCATAGACTG CCCGGAACTG 
GTGGGGAGGT GCTTTCTGGA GAGGATGGAG GAGTTCCAGA TCTACGAGAA GTACTGTCAG 
AACAAGCCAC GATCTGAGAG CCTGTGGAGA CAGTGCTCTG ACTGTCCCTT CTTCCAGGAG 
TGCCAGAAGA AGTTGGACCA CAAACTGAGC CTTGATTCCT ACCTGCTGAA GCCAGTGCAG 
AGAATAACTA AGTACCAGTT GCTGCTTAAG GAAATGTTGA AATACAGCAA GAACTGTGAG 
GGGGCAGAGG ACCTACAGGA GGCACTGAGC TCCATCCTGG GCATCCTCAA GGCAGTGAAT 
GACTCCATGC ACCTCATCGC CATCACTGGC TACGACGGTA ACCTGGGTGA TCTGGGGAAG 
CTGCTGATGC AAGGTTCCTT CAGTGTATGG ACAGACCACA AGAAGGGCCA CACCAAGGTC 
AAGGAGCTGG CCAGATTCAA GCCCATGCAG CGGCACCTTT TCCTGCATGA GAAGGCTGTG 
CTGTTCTGCA AGAAGAGGGA GGAGAATGGG GAGGGGTATG AGAAGGCCCC TTCCTACAGC 
TACAAGCAGT CATTGAATAT GACTGCTGTT GGCATCACAG AGAATGTGAA GGGTGATGCC 
AAGAAGTTCG AGATCTGGTA CAACGCAAGA GAGGAGGTTT ACATCATCCA GGCACCAACC 
CCTGAGATTA AAGCAGCATG GGTGAGTGAG ATTCGGAAGG TGCTGACCAG CCAGCTGCAG 
GCCTGCCGGG AAGCCAGCCA GCACAGGGCC CTGGAGCAGT CCCACAGCCT GCCTTTGCCC 
ACACCATCCA ACACCAGCCC CTCAAAAGGG AACACAAGGA ATGTCAAGAA GTCAGAAGAA 
AGAAAAACGG ACCCCCTGAG CCTGGAAGGA TATGTGAGCT CATCGTTGCC GAAGCCCCCT 
GAGAAAGGCA AAGGCTGGAG CAAAACATCC CACTCCCTGG AAGCCCCTGA GGAGGACGGA 
GGTTGGTCCA GTGCTGAGGA GCTTGTCAAT TCATCTGATG CAGAGGAAGA TGGAGTGGGC 
CCCAAGAAGC TGGTTCCTGG TAAATACACA GTGGTGATGG ACGATGAGAA AGGGGGCCTT 
GACACCCTTG CTGTGAGGAG CGGGGACATG GTAGAGGTGG TGGAAGAGGG CGCAGAGGGG 
CTCTGGTATG TCCGGGACCT GACCAGCAGC AAGGAGGGAT GGGTGCCAGC CAGCAGCCTT 
TCCACCCTCC TGGGCAAATC CAGTTCAGCC CAGTGTCTGA GCAGCTCAGA CTCCAGCCCA 
GGGTCAGCCG TGCTGAGCAA CTCCTCCAGC TGCAGCGAGG GCTGCCCCAC TCCCTTCTCC 
GACCTCCAGG GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Mcf2l
Entrez Gene ID	103746282
Full Name	MCF.2 cell line derived transforming sequence like
Gene Type	protein-coding
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Mcf2l cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene

Clones

gRNAs

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