Ibtk cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ibtk
Entrez Gene ID	103742308
Full Name	inhibitor of Bruton tyrosine kinase
Gene Type	protein-coding
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008843361.2	XP_008841583.1	inhibitor of Bruton tyrosine kinase isoform X1
XM_017800683.1	XP_017656172.1	inhibitor of Bruton tyrosine kinase isoform X3
XM_017800682.1	XP_017656171.1	inhibitor of Bruton tyrosine kinase isoform X2
XM_017800683.2	XP_017656172.1	inhibitor of Bruton tyrosine kinase isoform X3
XM_029568469.1	XP_029424329.1	inhibitor of Bruton tyrosine kinase isoform X4
XM_008843361.3	XP_008841583.1	inhibitor of Bruton tyrosine kinase isoform X1
XM_017800682.2	XP_017656171.1	inhibitor of Bruton tyrosine kinase isoform X2

Pathways from BioSystems
Gene Ontology

The following Ibtk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ibtk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONa133181	XM_029568469.1 Latest version!	Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ONa25776	XM_008843361.3 Latest version!	Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ONa25777	XM_017800682.2 Latest version!	Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ONa25778	XM_017800683.2 Latest version!	Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
ONa25778	XM_017800683.1	Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONa25776	XM_008843361.2	Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ONa25777	XM_017800682.1	Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ibtk ( XM_029568469.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029424329.1 Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ONa133181
Clone ID Related Accession (Same CDS sequence)	XM_029568469.1
Accession Version	XM_029568469.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	inhibitor of Bruton tyrosine kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008346901.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTG CCACCCCCGA CTGCACCCCC AAATGCCGAT CCCTGCAACA CGCTGCAGAC 
GTCCTTTCCG TGGTAACCAA GGGGAGTGAA AAGCAGATTA AGACCTTCCT CGCCAGCCAC 
TGCTACAGTG CTGCGACTGT CAAAGATGCC TTTGGCAGGA ATGCTGTCCA CCTGGCCTCC 
TCCTGTGGGA AGAAAGGCGT GCTAGACTGG CTTTTGGAGA GGGGAGTGGA TCCACTTGTG 
AAAGACAAGG AGTCAGGCTG GACTGCGCTG CACAGGAGCA TTTTCTATGG ACATATTGAT 
TGTCTTTGGT CTCTGTTGAA GCATGGTGTT AGTCTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTAAT GAAGGATAGA CCAGCTCATG TAGTATTTCA CAACACTGAC 
CCCACAGAAG TCTACACTTG GGGAGATAAT ACAAACTTTA CTCTGGGTCA TGGAAACCAG 
AACAGCAAAC ATCACCCAGA ACTGGTGGAT TTGTTCTCCA GGGGTGGCGT TTATATCAAA 
CAGATGCAGG CAAAGTACCT GAAAGGAAGA ACTGTCATTG GAGTAGCAGC AGGCAGGTTT 
CACACAGTTC TGTGGACTCG AGATGCCGTT TACACTATGG GCCTGAATGG TGGACAGCTG 
GGTTACTTAC TGGATCCCAA TGGAGAAAAG TGTGTCATAG CTCCTCGCCA GGTCTCTGCC 
CTTCATCATA AGGATGTTGC TCTGTCTTTG GTTGCTGCAA GTGATGGTGC CACAGTCTGT 
GTTACCACAA GAGGAGATAT ATACTTACTT GCAGACTATC AGTGCAAGAA GATAGCATCT 
AAACAACTGA ACTTGGAAAA GGTTCTTGTA TCTGGGGGTT GTATGGAATA CAAAGTCGAT 
CCTGAACATT TGAAAGAAAA TGGGGGTCAG AAAATCTGCG TTCTTGCTAT GGATGGAGCC 
GGAAGAGTAT TTTGCTGGAG ATCAGTCCAC AGTTCTCTGA AGCAATGCCG ATGGGCCTAT 
CCTCGCCAGG TGTTAATTTC TGATATTGCT TTAAATAGAA ATGAAGTTCT GTTTGTCACC 
CAGGATGGGG AAGGATTTAA AGGGAAATGG TTTGAAGGAA AAAGAAAGAA TTCTGAAAAG 
AAAGCAGACA TTTTACCAAA CCTACATCAT TCCTCATCAG ATGTGTCTTG TGTCTCTGAT 
ACAAATAGTG TGTATGAAAG AATTCGACTA GAGAAACTTA CTTTTGCCCA TAGAGCTGTT 
AGTGTCAGCA CAGACCCAAG TGGATGCAAC TTTGCAATCC TACAGTCAGA CCCTAAAACA 
AGCCTTTATG AAATTCCAGC TGTGTCTTCA TCATCCTTTT TTGAAGAATT TGGCAAACTC 
TTGAGGGAGA CAGATGAAAT GGACAACATT CACGATGTGA CATTTCAAGT TGGCACTAGA 
ACCTTCCCTG CTCATAAATA TGTTTTGGCT GTGCGTTCTG ACTTTTTCCA GAAATTGTTT 
CTTTCAGATG GTACTCCCTT GGAAGTCTCA GATGTGTACC GGAAAGAGGA AGATTCTGCA 
GGGTGCCATC TCTTTGTGGT GGAGAAGGTT CATCCTGATT TGTTTGAATG CCTTTTACAA 
TTCATGTATA CAGATACTTG TGACTTTTTA ACTCATGGTT TTAAGCCAAG GATATTCATA 
AACAAAAAGT CAGAAGAATA TCAGGGCTCC CTGAATTCTC TTATGAATAC GATGAATTGC 
CCTGAGGATG AGACCCAGAA GTCGGCGTTT GAGGTCTACA AAAGTAGCCA AGCTCACACA 
GTTAGTGAAA AGCAGAAAGG CAAACCCAAA TCTTCTAAAA AGGGAAGAAG TGCTAGTGAA 
GATGATCCTG TCAAGATGTT GCAAAATGTT GCAAAGAAAT TTGGCCTCAG TAATCTGAGT 
GGCAGGTTAG ATGGAGTCAG ATTTGAAAAT GAAAAAATTA ATGTTATTGC AAAGAAAACT 
GGCAATAAAT TGAAGCTAAG TCAAAAAAAA TGTTCATTTC TGTGTGATGT TATCATGAAA 
TCAGTGGATG GAAAGGAATA TCCTTGTCAT AAATGTGTCC TTTGTGCTAG ACTTGAGTAT 
TTTCATAGTA TGCTGAGTAG CTCGTGGATT GAGGCTTCGA GTGGTGCCGC TCTGGAAATG 
CCAGTACGTT CTGAGGTCCT GAGAGTGATT TTGGACTACC TCTACACTGA TGAAGCTCTG 
CTGATAAAAG AATCTCAAAA TGTGGATTTT ATTTGTAGTG TTCTTGTAGT GGCTGATCAG 
CTTCTGATAA CTCGATTAAA GGAGATTTGT GAAGTGGCAT TAACTGAAAA ACTTACCCTT 
AAGAATGCTG CTATGCTACT GGAATTTGCA GCAATGTACA ATGCTGAACA GTTGAAACTG 
TCTTGTTTAC AGTTTATAGG ACTGAATATG GCAGCTTTAC TTGAAGCAAG GTCTCTTGAT 
GTTTTAAGTG ATGATGTTTT GAAGGATCTT TCCATATTTT ACCGAAAAAT GATCCCAGCA 
ATGGATAGAA GAATCATTAC ACCATACCAA GATGGACCAG ATCTTAGCTG TTTGGAAGTA 
GAGGATGGAG CTGTCTTTTT GAAAGAAGAA ATAACTGTGG AGCAAAATTA TTCGGAAACT 
ATGTTTAAGA AGGCAAAAAC AAAAGCTAAA AAGAAGCCAC GGAAGCGCTC AGATAGTTCT 
GGAGGTTACA ACCTTTCAGA TATTATTCAG ACTCCACCAT CTGCAGGATT ATTGAAGTCT 
GGGAAGACCA GTTCTGTGGA ATCTCTTCCA GAACTGTTGA CCTCAGATTC TGAAGGAAGC 
TACACAGGAG TGGGTAGTCC CAGAGACCTG CAGTCCCCTG ATTTCACAGC AGGATTTAAT 
TCAGATAAGA TTGAGGGGAA AGCTAAATCG TATGTGAATG GTACGCCTCC CATGTGTTCT 
AGGGAAGGGT TCAAACCATG GGAAAAATCA CCAGCATCTA CATCTGCTCC CCAGCTCATC 
CCCAGTAACA GAGCTGACAC TCCAGGCTCA TCCATCTGGG TTGCGAGCTC TTGCAGTCCT 
GTGAGCCCTC CTGTTGTGGA TCTCAGAACC ATCATGGAAA TAGAAGAAAG TAGAAAAAAA 
TGTGGCACAA CACCAAAATT GAACTTAGGA AAAATTGTTT CTCATGGAGT TAAACTTTCT 
CAGAAACAAC GAAAAATGAT TGCATTGACA ACAAAAGACA ACCTTTCAGG AACAGGCAAC 
ATGGAAACAG TTGTTGTAGC TCCTTCCAAG CCTCCTAAGC CAGTGAATGC ACGGGCATCA 
TCTCTACAGT CCCCTTCATC CAAGTCATTC TGGGATTTCT TACTAGAAGA AAAAAAGTCT 
ATTATTGGCC ATAGTTCAGG TGATCAAGAT CATGTCAAAA AAGTTTGTTT TAAAGGAAAT 
GAAAATTCTC AGGCTCTAAA TGTTGTAAGG TGCTCAACTC ATGGAAGCCC AGGACTAGAA 
AACAACCTTA TTTCTGATTT ACCACTTCTA GACAGTCCCA ATCCCTGGCT GTCGTCTTCG 
TTGACTGCTT CTACCACAGC AGCTCCGGTC ACCTTCGCAT CAATTGTAGA AGAGGAGCTG 
CAGCAGGAAG CAGCTCTTAT CAGAAGTCGG GAAAAACCCT TGGCCCTGAT TCAGGTTGAG 
GAACATGCCA TACAAGATTT GCTGGTTTTC TATGAGGCGC TTGGCAACCC GGATGAGTTT 
GTCATCGTGG AAAGGACACC ACAGGGGCCA CTGGCAGTGC CTATGTGGAA CAAACACGGA 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029424329.1
CDS	522..4307
Translation	MNSATPDCTPKCRSLQHAADVLSVVTKGSEKQIKTFLASHCYSAATVKDAFGRNAVHLASSCGKKGVLDWLLERGVDPLVKDKESGWTALHRSIFYGHIDCLWSLLKHGVSLYMQDKEGLSPLDLLMKDRPAHVVFHNTDPTEVYTWGDNTNFTLGHGNQNSKHHPELVDLFSRGGVYIKQMQAKYLKGRTVIGVAAGRFHTVLWTRDAVYTMGLNGGQLGYLLDPNGEKCVIAPRQVSALHHKDVALSLVAASDGATVCVTTRGDIYLLADYQCKKIASKQLNLEKVLVSGGCMEYKVDPEHLKENGGQKICVLAMDGAGRVFCWRSVHSSLKQCRWAYPRQVLISDIALNRNEVLFVTQDGEGFKGKWFEGKRKNSEKKADILPNLHHSSSDVSCVSDTNSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDEMDNIHDVTFQVGTRTFPAHKYVLAVRSDFFQKLFLSDGTPLEVSDVYRKEEDSAGCHLFVVEKVHPDLFECLLQFMYTDTCDFLTHGFKPRIFINKKSEEYQGSLNSLMNTMNCPEDETQKSAFEVYKSSQAHTVSEKQKGKPKSSKKGRSASEDDPVKMLQNVAKKFGLSNLSGRLDGVRFENEKINVIAKKTGNKLKLSQKKCSFLCDVIMKSVDGKEYPCHKCVLCARLEYFHSMLSSSWIEASSGAALEMPVRSEVLRVILDYLYTDEALLIKESQNVDFICSVLVVADQLLITRLKEICEVALTEKLTLKNAAMLLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDDVLKDLSIFYRKMIPAMDRRIITPYQDGPDLSCLEVEDGAVFLKEEITVEQNYSETMFKKAKTKAKKKPRKRSDSSGGYNLSDIIQTPPSAGLLKSGKTSSVESLPELLTSDSEGSYTGVGSPRDLQSPDFTAGFNSDKIEGKAKSYVNGTPPMCSREGFKPWEKSPASTSAPQLIPSNRADTPGSSIWVASSCSPVSPPVVDLRTIMEIEESRKKCGTTPKLNLGKIVSHGVKLSQKQRKMIALTTKDNLSGTGNMETVVVAPSKPPKPVNARASSLQSPSSKSFWDFLLEEKKSIIGHSSGDQDHVKKVCFKGNENSQALNVVRCSTHGSPGLENNLISDLPLLDSPNPWLSSSLTASTTAAPVTFASIVEEELQQEAALIRSREKPLALIQVEEHAIQDLLVFYEALGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_029568469.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029568469.1

ORF Insert Sequence:

ATGAACTCTG CCACCCCCGA CTGCACCCCC AAATGCCGAT CCCTGCAACA CGCTGCAGAC 
GTCCTTTCCG TGGTAACCAA GGGGAGTGAA AAGCAGATTA AGACCTTCCT CGCCAGCCAC 
TGCTACAGTG CTGCGACTGT CAAAGATGCC TTTGGCAGGA ATGCTGTCCA CCTGGCCTCC 
TCCTGTGGGA AGAAAGGCGT GCTAGACTGG CTTTTGGAGA GGGGAGTGGA TCCACTTGTG 
AAAGACAAGG AGTCAGGCTG GACTGCGCTG CACAGGAGCA TTTTCTATGG ACATATTGAT 
TGTCTTTGGT CTCTGTTGAA GCATGGTGTT AGTCTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTAAT GAAGGATAGA CCAGCTCATG TAGTATTTCA CAACACTGAC 
CCCACAGAAG TCTACACTTG GGGAGATAAT ACAAACTTTA CTCTGGGTCA TGGAAACCAG 
AACAGCAAAC ATCACCCAGA ACTGGTGGAT TTGTTCTCCA GGGGTGGCGT TTATATCAAA 
CAGATGCAGG CAAAGTACCT GAAAGGAAGA ACTGTCATTG GAGTAGCAGC AGGCAGGTTT 
CACACAGTTC TGTGGACTCG AGATGCCGTT TACACTATGG GCCTGAATGG TGGACAGCTG 
GGTTACTTAC TGGATCCCAA TGGAGAAAAG TGTGTCATAG CTCCTCGCCA GGTCTCTGCC 
CTTCATCATA AGGATGTTGC TCTGTCTTTG GTTGCTGCAA GTGATGGTGC CACAGTCTGT 
GTTACCACAA GAGGAGATAT ATACTTACTT GCAGACTATC AGTGCAAGAA GATAGCATCT 
AAACAACTGA ACTTGGAAAA GGTTCTTGTA TCTGGGGGTT GTATGGAATA CAAAGTCGAT 
CCTGAACATT TGAAAGAAAA TGGGGGTCAG AAAATCTGCG TTCTTGCTAT GGATGGAGCC 
GGAAGAGTAT TTTGCTGGAG ATCAGTCCAC AGTTCTCTGA AGCAATGCCG ATGGGCCTAT 
CCTCGCCAGG TGTTAATTTC TGATATTGCT TTAAATAGAA ATGAAGTTCT GTTTGTCACC 
CAGGATGGGG AAGGATTTAA AGGGAAATGG TTTGAAGGAA AAAGAAAGAA TTCTGAAAAG 
AAAGCAGACA TTTTACCAAA CCTACATCAT TCCTCATCAG ATGTGTCTTG TGTCTCTGAT 
ACAAATAGTG TGTATGAAAG AATTCGACTA GAGAAACTTA CTTTTGCCCA TAGAGCTGTT 
AGTGTCAGCA CAGACCCAAG TGGATGCAAC TTTGCAATCC TACAGTCAGA CCCTAAAACA 
AGCCTTTATG AAATTCCAGC TGTGTCTTCA TCATCCTTTT TTGAAGAATT TGGCAAACTC 
TTGAGGGAGA CAGATGAAAT GGACAACATT CACGATGTGA CATTTCAAGT TGGCACTAGA 
ACCTTCCCTG CTCATAAATA TGTTTTGGCT GTGCGTTCTG ACTTTTTCCA GAAATTGTTT 
CTTTCAGATG GTACTCCCTT GGAAGTCTCA GATGTGTACC GGAAAGAGGA AGATTCTGCA 
GGGTGCCATC TCTTTGTGGT GGAGAAGGTT CATCCTGATT TGTTTGAATG CCTTTTACAA 
TTCATGTATA CAGATACTTG TGACTTTTTA ACTCATGGTT TTAAGCCAAG GATATTCATA 
AACAAAAAGT CAGAAGAATA TCAGGGCTCC CTGAATTCTC TTATGAATAC GATGAATTGC 
CCTGAGGATG AGACCCAGAA GTCGGCGTTT GAGGTCTACA AAAGTAGCCA AGCTCACACA 
GTTAGTGAAA AGCAGAAAGG CAAACCCAAA TCTTCTAAAA AGGGAAGAAG TGCTAGTGAA 
GATGATCCTG TCAAGATGTT GCAAAATGTT GCAAAGAAAT TTGGCCTCAG TAATCTGAGT 
GGCAGGTTAG ATGGAGTCAG ATTTGAAAAT GAAAAAATTA ATGTTATTGC AAAGAAAACT 
GGCAATAAAT TGAAGCTAAG TCAAAAAAAA TGTTCATTTC TGTGTGATGT TATCATGAAA 
TCAGTGGATG GAAAGGAATA TCCTTGTCAT AAATGTGTCC TTTGTGCTAG ACTTGAGTAT 
TTTCATAGTA TGCTGAGTAG CTCGTGGATT GAGGCTTCGA GTGGTGCCGC TCTGGAAATG 
CCAGTACGTT CTGAGGTCCT GAGAGTGATT TTGGACTACC TCTACACTGA TGAAGCTCTG 
CTGATAAAAG AATCTCAAAA TGTGGATTTT ATTTGTAGTG TTCTTGTAGT GGCTGATCAG 
CTTCTGATAA CTCGATTAAA GGAGATTTGT GAAGTGGCAT TAACTGAAAA ACTTACCCTT 
AAGAATGCTG CTATGCTACT GGAATTTGCA GCAATGTACA ATGCTGAACA GTTGAAACTG 
TCTTGTTTAC AGTTTATAGG ACTGAATATG GCAGCTTTAC TTGAAGCAAG GTCTCTTGAT 
GTTTTAAGTG ATGATGTTTT GAAGGATCTT TCCATATTTT ACCGAAAAAT GATCCCAGCA 
ATGGATAGAA GAATCATTAC ACCATACCAA GATGGACCAG ATCTTAGCTG TTTGGAAGTA 
GAGGATGGAG CTGTCTTTTT GAAAGAAGAA ATAACTGTGG AGCAAAATTA TTCGGAAACT 
ATGTTTAAGA AGGCAAAAAC AAAAGCTAAA AAGAAGCCAC GGAAGCGCTC AGATAGTTCT 
GGAGGTTACA ACCTTTCAGA TATTATTCAG ACTCCACCAT CTGCAGGATT ATTGAAGTCT 
GGGAAGACCA GTTCTGTGGA ATCTCTTCCA GAACTGTTGA CCTCAGATTC TGAAGGAAGC 
TACACAGGAG TGGGTAGTCC CAGAGACCTG CAGTCCCCTG ATTTCACAGC AGGATTTAAT 
TCAGATAAGA TTGAGGGGAA AGCTAAATCG TATGTGAATG GTACGCCTCC CATGTGTTCT 
AGGGAAGGGT TCAAACCATG GGAAAAATCA CCAGCATCTA CATCTGCTCC CCAGCTCATC 
CCCAGTAACA GAGCTGACAC TCCAGGCTCA TCCATCTGGG TTGCGAGCTC TTGCAGTCCT 
GTGAGCCCTC CTGTTGTGGA TCTCAGAACC ATCATGGAAA TAGAAGAAAG TAGAAAAAAA 
TGTGGCACAA CACCAAAATT GAACTTAGGA AAAATTGTTT CTCATGGAGT TAAACTTTCT 
CAGAAACAAC GAAAAATGAT TGCATTGACA ACAAAAGACA ACCTTTCAGG AACAGGCAAC 
ATGGAAACAG TTGTTGTAGC TCCTTCCAAG CCTCCTAAGC CAGTGAATGC ACGGGCATCA 
TCTCTACAGT CCCCTTCATC CAAGTCATTC TGGGATTTCT TACTAGAAGA AAAAAAGTCT 
ATTATTGGCC ATAGTTCAGG TGATCAAGAT CATGTCAAAA AAGTTTGTTT TAAAGGAAAT 
GAAAATTCTC AGGCTCTAAA TGTTGTAAGG TGCTCAACTC ATGGAAGCCC AGGACTAGAA 
AACAACCTTA TTTCTGATTT ACCACTTCTA GACAGTCCCA ATCCCTGGCT GTCGTCTTCG 
TTGACTGCTT CTACCACAGC AGCTCCGGTC ACCTTCGCAT CAATTGTAGA AGAGGAGCTG 
CAGCAGGAAG CAGCTCTTAT CAGAAGTCGG GAAAAACCCT TGGCCCTGAT TCAGGTTGAG 
GAACATGCCA TACAAGATTT GCTGGTTTTC TATGAGGCGC TTGGCAACCC GGATGAGTTT 
GTCATCGTGG AAAGGACACC ACAGGGGCCA CTGGCAGTGC CTATGTGGAA CAAACACGGA 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ibtk ( XM_008843361.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008841583.1 Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONa25776
Clone ID Related Accession (Same CDS sequence)	XM_008843361.2 , XM_008843361.3
Accession Version	XM_008843361.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	inhibitor of Bruton tyrosine kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008346901.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008843361.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTG CCACCCCCGA CTGCACCCCC AAATGCCGAT CCCTGCAACA CGCTGCAGAC 
GTCCTTTCCG TGGTAACCAA GGGGAGTGAA AAGCAGATTA AGACCTTCCT CGCCAGCCAC 
TGCTACAGTG CTGCGACTGT CAAAGATGCC TTTGGCAGGA ATGCTGTCCA CCTGGCCTCC 
TCCTGTGGGA AGAAAGGCGT GCTAGACTGG CTTTTGGAGA GGGGAGTGGA TCCACTTGTG 
AAAGACAAGG AGTCAGGCTG GACTGCGCTG CACAGGAGCA TTTTCTATGG ACATATTGAT 
TGTCTTTGGT CTCTGTTGAA GCATGGTGTT AGTCTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTAAT GAAGGATAGA CCAGCTCATG TAGTATTTCA CAACACTGAC 
CCCACAGAAG TCTACACTTG GGGAGATAAT ACAAACTTTA CTCTGGGTCA TGGAAACCAG 
AACAGCAAAC ATCACCCAGA ACTGGTGGAT TTGTTCTCCA GGGGTGGCGT TTATATCAAA 
CAGGTGGTGC TTTGCAAATT TCATTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC 
TGTGGGCATG GTCCAGGAGG AAGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGACTTGTGG AAGGACTGAG TGGTCATAAC TGTTCCCAGG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACCTTTG GTCTCAATAC TTTTCACCAA 
TTAGGAATTA CTCCTCCTCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC 
CTGAAAGGAA GAACTGTCAT TGGAGTAGCA GCAGGCAGGT TTCACACAGT TCTGTGGACT 
CGAGATGCCG TTTACACTAT GGGCCTGAAT GGTGGACAGC TGGGTTACTT ACTGGATCCC 
AATGGAGAAA AGTGTGTCAT AGCTCCTCGC CAGGTCTCTG CCCTTCATCA TAAGGATGTT 
GCTCTGTCTT TGGTTGCTGC AAGTGATGGT GCCACAGTCT GTGTTACCAC AAGAGGAGAT 
ATATACTTAC TTGCAGACTA TCAGTGCAAG AAGATAGCAT CTAAACAACT GAACTTGGAA 
AAGGTTCTTG TATCTGGGGG TTGTATGGAA TACAAAGTCG ATCCTGAACA TTTGAAAGAA 
AATGGGGGTC AGAAAATCTG CGTTCTTGCT ATGGATGGAG CCGGAAGAGT ATTTTGCTGG 
AGATCAGTCC ACAGTTCTCT GAAGCAATGC CGATGGGCCT ATCCTCGCCA GGTGTTAATT 
TCTGATATTG CTTTAAATAG AAATGAAGTT CTGTTTGTCA CCCAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGG AAAAAGAAAG AATTCTGAAA AGAAAGCAGA CATTTTACCA 
AACCTACATC ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATACAAATAG TGTGTATGAA 
AGAATTCGAC TAGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGACCCA 
AGTGGATGCA ACTTTGCAAT CCTACAGTCA GACCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCTGTGTCTT CATCATCCTT TTTTGAAGAA TTTGGCAAAC TCTTGAGGGA GACAGATGAA 
ATGGACAACA TTCACGATGT GACATTTCAA GTTGGCACTA GAACCTTCCC TGCTCATAAA 
TATGTTTTGG CTGTGCGTTC TGACTTTTTC CAGAAATTGT TTCTTTCAGA TGGTACTCCC 
TTGGAAGTCT CAGATGTGTA CCGGAAAGAG GAAGATTCTG CAGGGTGCCA TCTCTTTGTG 
GTGGAGAAGG TTCATCCTGA TTTGTTTGAA TGCCTTTTAC AATTCATGTA TACAGATACT 
TGTGACTTTT TAACTCATGG TTTTAAGCCA AGGATATTCA TAAACAAAAA GTCAGAAGAA 
TATCAGGGCT CCCTGAATTC TCTTATGAAT ACGATGAATT GCCCTGAGGA TGAGACCCAG 
AAGTCGGCGT TTGAGGTCTA CAAAAGTAGC CAAGCTCACA CAGTTAGTGA AAAGCAGAAA 
GGCAAACCCA AATCTTCTAA AAAGGGAAGA AGTGCTAGTG AAGATGATCC TGTCAAGATG 
TTGCAAAATG TTGCAAAGAA ATTTGGCCTC AGTAATCTGA GTGGCAGGTT AGATGGAGTC 
AGATTTGAAA ATGAAAAAAT TAATGTTATT GCAAAGAAAA CTGGCAATAA ATTGAAGCTA 
AGTCAAAAAA AATGTTCATT TCTGTGTGAT GTTATCATGA AATCAGTGGA TGGAAAGGAA 
TATCCTTGTC ATAAATGTGT CCTTTGTGCT AGACTTGAGT ATTTTCATAG TATGCTGAGT 
AGCTCGTGGA TTGAGGCTTC GAGTGGTGCC GCTCTGGAAA TGCCAGTACG TTCTGAGGTC 
CTGAGAGTGA TTTTGGACTA CCTCTACACT GATGAAGCTC TGCTGATAAA AGAATCTCAA 
AATGTGGATT TTATTTGTAG TGTTCTTGTA GTGGCTGATC AGCTTCTGAT AACTCGATTA 
AAGGAGATTT GTGAAGTGGC ATTAACTGAA AAACTTACCC TTAAGAATGC TGCTATGCTA 
CTGGAATTTG CAGCAATGTA CAATGCTGAA CAGTTGAAAC TGTCTTGTTT ACAGTTTATA 
GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGGTCTCTTG ATGTTTTAAG TGATGATGTT 
TTGAAGGATC TTTCCATATT TTACCGAAAA ATGATCCCAG CAATGGATAG AAGAATCATT 
ACACCATACC AAGATGGACC AGATCTTAGC TGTTTGGAAG TAGAGGATGG AGCTGTCTTT 
TTGAAAGAAG AAATAACTGT GGAGCAAAAT TATTCGGAAA CTATGTTTAA GAAGGCAAAA 
ACAAAAGCTA AAAAGAAGCC ACGGAAGCGC TCAGATAGTT CTGGAGGTTA CAACCTTTCA 
GATATTATTC AGACTCCACC ATCTGCAGGA TTATTGAAGT CTGGGAAGAC CAGTTCTGTG 
GAATCTCTTC CAGAACTGTT GACCTCAGAT TCTGAAGGAA GCTACACAGG AGTGGGTAGT 
CCCAGAGACC TGCAGTCCCC TGATTTCACA GCAGGATTTA ATTCAGATAA GATTGAGGGG 
AAAGCTAAAT CGTATGTGAA TGGTACGCCT CCCATGTGTT CTAGGGAAGG GTTCAAACCA 
TGGGAAAAAT CACCAGCATC TACATCTGCT CCCCAGCTCA TCCCCAGTAA CAGAGCTGAC 
ACTCCAGGCT CATCCATCTG GGTTGCGAGC TCTTGCAGTC CTGTGAGCCC TCCTGTTGTG 
GATCTCAGAA CCATCATGGA AATAGAAGAA AGTAGAAAAA AATGTGGCAC AACACCAAAA 
TTGAACTTAG GAAAAATTGT TTCTCATGGA GTTAAACTTT CTCAGAAACA ACGAAAAATG 
ATTGCATTGA CAACAAAAGA CAACCTTTCA GGAACAGGCA ACATGGAAAC AGTTGTTGTA 
GCTCCTTCCA AGCCTCCTAA GCCAGTGAAT GCACGGGCAT CATCTCTACA GTCCCCTTCA 
TCCAAGTCAT TCTGGGATTT CTTACTAGAA GAAAAAAAGT CTATTATTGG CCATAGTTCA 
GGTGATCAAG ATCATGTCAA AAAAGTTTGT TTTAAAGGAA ATGAAAATTC TCAGGCTCTA 
AATGTTGTAA GGTGCTCAAC TCATGGAAGC CCAGGACTAG AAAACAACCT TATTTCTGAT 
TTACCACTTC TAGACAGTCC CAATCCCTGG CTGTCGTCTT CGTTGACTGC TTCTACCACA 
GCAGCTCCGG TCACCTTCGC ATCAATTGTA GAAGAGGAGC TGCAGCAGGA AGCAGCTCTT 
ATCAGAAGTC GGGAAAAACC CTTGGCCCTG ATTCAGGTTG AGGAACATGC CATACAAGAT 
TTGCTGGTTT TCTATGAGGC GCTTGGCAAC CCGGATGAGT TTGTCATCGT GGAAAGGACA 
CCACAGGGGC CACTGGCAGT GCCTATGTGG AACAAACACG GATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008841583.1
CDS	523..4590
Translation	MNSATPDCTPKCRSLQHAADVLSVVTKGSEKQIKTFLASHCYSAATVKDAFGRNAVHLASSCGKKGVLDWLLERGVDPLVKDKESGWTALHRSIFYGHIDCLWSLLKHGVSLYMQDKEGLSPLDLLMKDRPAHVVFHNTDPTEVYTWGDNTNFTLGHGNQNSKHHPELVDLFSRGGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLVPRLVEGLSGHNCSQVAAAKDHTVVLTEDGCVYTFGLNTFHQLGITPPPSSCNVPRQMQAKYLKGRTVIGVAAGRFHTVLWTRDAVYTMGLNGGQLGYLLDPNGEKCVIAPRQVSALHHKDVALSLVAASDGATVCVTTRGDIYLLADYQCKKIASKQLNLEKVLVSGGCMEYKVDPEHLKENGGQKICVLAMDGAGRVFCWRSVHSSLKQCRWAYPRQVLISDIALNRNEVLFVTQDGEGFKGKWFEGKRKNSEKKADILPNLHHSSSDVSCVSDTNSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDEMDNIHDVTFQVGTRTFPAHKYVLAVRSDFFQKLFLSDGTPLEVSDVYRKEEDSAGCHLFVVEKVHPDLFECLLQFMYTDTCDFLTHGFKPRIFINKKSEEYQGSLNSLMNTMNCPEDETQKSAFEVYKSSQAHTVSEKQKGKPKSSKKGRSASEDDPVKMLQNVAKKFGLSNLSGRLDGVRFENEKINVIAKKTGNKLKLSQKKCSFLCDVIMKSVDGKEYPCHKCVLCARLEYFHSMLSSSWIEASSGAALEMPVRSEVLRVILDYLYTDEALLIKESQNVDFICSVLVVADQLLITRLKEICEVALTEKLTLKNAAMLLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDDVLKDLSIFYRKMIPAMDRRIITPYQDGPDLSCLEVEDGAVFLKEEITVEQNYSETMFKKAKTKAKKKPRKRSDSSGGYNLSDIIQTPPSAGLLKSGKTSSVESLPELLTSDSEGSYTGVGSPRDLQSPDFTAGFNSDKIEGKAKSYVNGTPPMCSREGFKPWEKSPASTSAPQLIPSNRADTPGSSIWVASSCSPVSPPVVDLRTIMEIEESRKKCGTTPKLNLGKIVSHGVKLSQKQRKMIALTTKDNLSGTGNMETVVVAPSKPPKPVNARASSLQSPSSKSFWDFLLEEKKSIIGHSSGDQDHVKKVCFKGNENSQALNVVRCSTHGSPGLENNLISDLPLLDSPNPWLSSSLTASTTAAPVTFASIVEEELQQEAALIRSREKPLALIQVEEHAIQDLLVFYEALGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_008843361.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008843361.2

ORF Insert Sequence:

ATGAACTCTG CCACCCCCGA CTGCACCCCC AAATGCCGAT CCCTGCAACA CGCTGCAGAC 
GTCCTTTCCG TGGTAACCAA GGGGAGTGAA AAGCAGATTA AGACCTTCCT CGCCAGCCAC 
TGCTACAGTG CTGCGACTGT CAAAGATGCC TTTGGCAGGA ATGCTGTCCA CCTGGCCTCC 
TCCTGTGGGA AGAAAGGCGT GCTAGACTGG CTTTTGGAGA GGGGAGTGGA TCCACTTGTG 
AAAGACAAGG AGTCAGGCTG GACTGCGCTG CACAGGAGCA TTTTCTATGG ACATATTGAT 
TGTCTTTGGT CTCTGTTGAA GCATGGTGTT AGTCTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTAAT GAAGGATAGA CCAGCTCATG TAGTATTTCA CAACACTGAC 
CCCACAGAAG TCTACACTTG GGGAGATAAT ACAAACTTTA CTCTGGGTCA TGGAAACCAG 
AACAGCAAAC ATCACCCAGA ACTGGTGGAT TTGTTCTCCA GGGGTGGCGT TTATATCAAA 
CAGGTGGTGC TTTGCAAATT TCATTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC 
TGTGGGCATG GTCCAGGAGG AAGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGACTTGTGG AAGGACTGAG TGGTCATAAC TGTTCCCAGG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACCTTTG GTCTCAATAC TTTTCACCAA 
TTAGGAATTA CTCCTCCTCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC 
CTGAAAGGAA GAACTGTCAT TGGAGTAGCA GCAGGCAGGT TTCACACAGT TCTGTGGACT 
CGAGATGCCG TTTACACTAT GGGCCTGAAT GGTGGACAGC TGGGTTACTT ACTGGATCCC 
AATGGAGAAA AGTGTGTCAT AGCTCCTCGC CAGGTCTCTG CCCTTCATCA TAAGGATGTT 
GCTCTGTCTT TGGTTGCTGC AAGTGATGGT GCCACAGTCT GTGTTACCAC AAGAGGAGAT 
ATATACTTAC TTGCAGACTA TCAGTGCAAG AAGATAGCAT CTAAACAACT GAACTTGGAA 
AAGGTTCTTG TATCTGGGGG TTGTATGGAA TACAAAGTCG ATCCTGAACA TTTGAAAGAA 
AATGGGGGTC AGAAAATCTG CGTTCTTGCT ATGGATGGAG CCGGAAGAGT ATTTTGCTGG 
AGATCAGTCC ACAGTTCTCT GAAGCAATGC CGATGGGCCT ATCCTCGCCA GGTGTTAATT 
TCTGATATTG CTTTAAATAG AAATGAAGTT CTGTTTGTCA CCCAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGG AAAAAGAAAG AATTCTGAAA AGAAAGCAGA CATTTTACCA 
AACCTACATC ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATACAAATAG TGTGTATGAA 
AGAATTCGAC TAGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGACCCA 
AGTGGATGCA ACTTTGCAAT CCTACAGTCA GACCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCTGTGTCTT CATCATCCTT TTTTGAAGAA TTTGGCAAAC TCTTGAGGGA GACAGATGAA 
ATGGACAACA TTCACGATGT GACATTTCAA GTTGGCACTA GAACCTTCCC TGCTCATAAA 
TATGTTTTGG CTGTGCGTTC TGACTTTTTC CAGAAATTGT TTCTTTCAGA TGGTACTCCC 
TTGGAAGTCT CAGATGTGTA CCGGAAAGAG GAAGATTCTG CAGGGTGCCA TCTCTTTGTG 
GTGGAGAAGG TTCATCCTGA TTTGTTTGAA TGCCTTTTAC AATTCATGTA TACAGATACT 
TGTGACTTTT TAACTCATGG TTTTAAGCCA AGGATATTCA TAAACAAAAA GTCAGAAGAA 
TATCAGGGCT CCCTGAATTC TCTTATGAAT ACGATGAATT GCCCTGAGGA TGAGACCCAG 
AAGTCGGCGT TTGAGGTCTA CAAAAGTAGC CAAGCTCACA CAGTTAGTGA AAAGCAGAAA 
GGCAAACCCA AATCTTCTAA AAAGGGAAGA AGTGCTAGTG AAGATGATCC TGTCAAGATG 
TTGCAAAATG TTGCAAAGAA ATTTGGCCTC AGTAATCTGA GTGGCAGGTT AGATGGAGTC 
AGATTTGAAA ATGAAAAAAT TAATGTTATT GCAAAGAAAA CTGGCAATAA ATTGAAGCTA 
AGTCAAAAAA AATGTTCATT TCTGTGTGAT GTTATCATGA AATCAGTGGA TGGAAAGGAA 
TATCCTTGTC ATAAATGTGT CCTTTGTGCT AGACTTGAGT ATTTTCATAG TATGCTGAGT 
AGCTCGTGGA TTGAGGCTTC GAGTGGTGCC GCTCTGGAAA TGCCAGTACG TTCTGAGGTC 
CTGAGAGTGA TTTTGGACTA CCTCTACACT GATGAAGCTC TGCTGATAAA AGAATCTCAA 
AATGTGGATT TTATTTGTAG TGTTCTTGTA GTGGCTGATC AGCTTCTGAT AACTCGATTA 
AAGGAGATTT GTGAAGTGGC ATTAACTGAA AAACTTACCC TTAAGAATGC TGCTATGCTA 
CTGGAATTTG CAGCAATGTA CAATGCTGAA CAGTTGAAAC TGTCTTGTTT ACAGTTTATA 
GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGGTCTCTTG ATGTTTTAAG TGATGATGTT 
TTGAAGGATC TTTCCATATT TTACCGAAAA ATGATCCCAG CAATGGATAG AAGAATCATT 
ACACCATACC AAGATGGACC AGATCTTAGC TGTTTGGAAG TAGAGGATGG AGCTGTCTTT 
TTGAAAGAAG AAATAACTGT GGAGCAAAAT TATTCGGAAA CTATGTTTAA GAAGGCAAAA 
ACAAAAGCTA AAAAGAAGCC ACGGAAGCGC TCAGATAGTT CTGGAGGTTA CAACCTTTCA 
GATATTATTC AGACTCCACC ATCTGCAGGA TTATTGAAGT CTGGGAAGAC CAGTTCTGTG 
GAATCTCTTC CAGAACTGTT GACCTCAGAT TCTGAAGGAA GCTACACAGG AGTGGGTAGT 
CCCAGAGACC TGCAGTCCCC TGATTTCACA GCAGGATTTA ATTCAGATAA GATTGAGGGG 
AAAGCTAAAT CGTATGTGAA TGGTACGCCT CCCATGTGTT CTAGGGAAGG GTTCAAACCA 
TGGGAAAAAT CACCAGCATC TACATCTGCT CCCCAGCTCA TCCCCAGTAA CAGAGCTGAC 
ACTCCAGGCT CATCCATCTG GGTTGCGAGC TCTTGCAGTC CTGTGAGCCC TCCTGTTGTG 
GATCTCAGAA CCATCATGGA AATAGAAGAA AGTAGAAAAA AATGTGGCAC AACACCAAAA 
TTGAACTTAG GAAAAATTGT TTCTCATGGA GTTAAACTTT CTCAGAAACA ACGAAAAATG 
ATTGCATTGA CAACAAAAGA CAACCTTTCA GGAACAGGCA ACATGGAAAC AGTTGTTGTA 
GCTCCTTCCA AGCCTCCTAA GCCAGTGAAT GCACGGGCAT CATCTCTACA GTCCCCTTCA 
TCCAAGTCAT TCTGGGATTT CTTACTAGAA GAAAAAAAGT CTATTATTGG CCATAGTTCA 
GGTGATCAAG ATCATGTCAA AAAAGTTTGT TTTAAAGGAA ATGAAAATTC TCAGGCTCTA 
AATGTTGTAA GGTGCTCAAC TCATGGAAGC CCAGGACTAG AAAACAACCT TATTTCTGAT 
TTACCACTTC TAGACAGTCC CAATCCCTGG CTGTCGTCTT CGTTGACTGC TTCTACCACA 
GCAGCTCCGG TCACCTTCGC ATCAATTGTA GAAGAGGAGC TGCAGCAGGA AGCAGCTCTT 
ATCAGAAGTC GGGAAAAACC CTTGGCCCTG ATTCAGGTTG AGGAACATGC CATACAAGAT 
TTGCTGGTTT TCTATGAGGC GCTTGGCAAC CCGGATGAGT TTGTCATCGT GGAAAGGACA 
CCACAGGGGC CACTGGCAGT GCCTATGTGG AACAAACACG GATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ibtk ( XM_008843361.3 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008841583.1 Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X1, mRNA.

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CloneID	ONa25776
Clone ID Related Accession (Same CDS sequence)	XM_008843361.2 , XM_008843361.3
Accession Version	XM_008843361.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	inhibitor of Bruton tyrosine kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008346901.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008843361.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTG CCACCCCCGA CTGCACCCCC AAATGCCGAT CCCTGCAACA CGCTGCAGAC 
GTCCTTTCCG TGGTAACCAA GGGGAGTGAA AAGCAGATTA AGACCTTCCT CGCCAGCCAC 
TGCTACAGTG CTGCGACTGT CAAAGATGCC TTTGGCAGGA ATGCTGTCCA CCTGGCCTCC 
TCCTGTGGGA AGAAAGGCGT GCTAGACTGG CTTTTGGAGA GGGGAGTGGA TCCACTTGTG 
AAAGACAAGG AGTCAGGCTG GACTGCGCTG CACAGGAGCA TTTTCTATGG ACATATTGAT 
TGTCTTTGGT CTCTGTTGAA GCATGGTGTT AGTCTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTAAT GAAGGATAGA CCAGCTCATG TAGTATTTCA CAACACTGAC 
CCCACAGAAG TCTACACTTG GGGAGATAAT ACAAACTTTA CTCTGGGTCA TGGAAACCAG 
AACAGCAAAC ATCACCCAGA ACTGGTGGAT TTGTTCTCCA GGGGTGGCGT TTATATCAAA 
CAGGTGGTGC TTTGCAAATT TCATTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC 
TGTGGGCATG GTCCAGGAGG AAGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGACTTGTGG AAGGACTGAG TGGTCATAAC TGTTCCCAGG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACCTTTG GTCTCAATAC TTTTCACCAA 
TTAGGAATTA CTCCTCCTCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC 
CTGAAAGGAA GAACTGTCAT TGGAGTAGCA GCAGGCAGGT TTCACACAGT TCTGTGGACT 
CGAGATGCCG TTTACACTAT GGGCCTGAAT GGTGGACAGC TGGGTTACTT ACTGGATCCC 
AATGGAGAAA AGTGTGTCAT AGCTCCTCGC CAGGTCTCTG CCCTTCATCA TAAGGATGTT 
GCTCTGTCTT TGGTTGCTGC AAGTGATGGT GCCACAGTCT GTGTTACCAC AAGAGGAGAT 
ATATACTTAC TTGCAGACTA TCAGTGCAAG AAGATAGCAT CTAAACAACT GAACTTGGAA 
AAGGTTCTTG TATCTGGGGG TTGTATGGAA TACAAAGTCG ATCCTGAACA TTTGAAAGAA 
AATGGGGGTC AGAAAATCTG CGTTCTTGCT ATGGATGGAG CCGGAAGAGT ATTTTGCTGG 
AGATCAGTCC ACAGTTCTCT GAAGCAATGC CGATGGGCCT ATCCTCGCCA GGTGTTAATT 
TCTGATATTG CTTTAAATAG AAATGAAGTT CTGTTTGTCA CCCAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGG AAAAAGAAAG AATTCTGAAA AGAAAGCAGA CATTTTACCA 
AACCTACATC ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATACAAATAG TGTGTATGAA 
AGAATTCGAC TAGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGACCCA 
AGTGGATGCA ACTTTGCAAT CCTACAGTCA GACCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCTGTGTCTT CATCATCCTT TTTTGAAGAA TTTGGCAAAC TCTTGAGGGA GACAGATGAA 
ATGGACAACA TTCACGATGT GACATTTCAA GTTGGCACTA GAACCTTCCC TGCTCATAAA 
TATGTTTTGG CTGTGCGTTC TGACTTTTTC CAGAAATTGT TTCTTTCAGA TGGTACTCCC 
TTGGAAGTCT CAGATGTGTA CCGGAAAGAG GAAGATTCTG CAGGGTGCCA TCTCTTTGTG 
GTGGAGAAGG TTCATCCTGA TTTGTTTGAA TGCCTTTTAC AATTCATGTA TACAGATACT 
TGTGACTTTT TAACTCATGG TTTTAAGCCA AGGATATTCA TAAACAAAAA GTCAGAAGAA 
TATCAGGGCT CCCTGAATTC TCTTATGAAT ACGATGAATT GCCCTGAGGA TGAGACCCAG 
AAGTCGGCGT TTGAGGTCTA CAAAAGTAGC CAAGCTCACA CAGTTAGTGA AAAGCAGAAA 
GGCAAACCCA AATCTTCTAA AAAGGGAAGA AGTGCTAGTG AAGATGATCC TGTCAAGATG 
TTGCAAAATG TTGCAAAGAA ATTTGGCCTC AGTAATCTGA GTGGCAGGTT AGATGGAGTC 
AGATTTGAAA ATGAAAAAAT TAATGTTATT GCAAAGAAAA CTGGCAATAA ATTGAAGCTA 
AGTCAAAAAA AATGTTCATT TCTGTGTGAT GTTATCATGA AATCAGTGGA TGGAAAGGAA 
TATCCTTGTC ATAAATGTGT CCTTTGTGCT AGACTTGAGT ATTTTCATAG TATGCTGAGT 
AGCTCGTGGA TTGAGGCTTC GAGTGGTGCC GCTCTGGAAA TGCCAGTACG TTCTGAGGTC 
CTGAGAGTGA TTTTGGACTA CCTCTACACT GATGAAGCTC TGCTGATAAA AGAATCTCAA 
AATGTGGATT TTATTTGTAG TGTTCTTGTA GTGGCTGATC AGCTTCTGAT AACTCGATTA 
AAGGAGATTT GTGAAGTGGC ATTAACTGAA AAACTTACCC TTAAGAATGC TGCTATGCTA 
CTGGAATTTG CAGCAATGTA CAATGCTGAA CAGTTGAAAC TGTCTTGTTT ACAGTTTATA 
GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGGTCTCTTG ATGTTTTAAG TGATGATGTT 
TTGAAGGATC TTTCCATATT TTACCGAAAA ATGATCCCAG CAATGGATAG AAGAATCATT 
ACACCATACC AAGATGGACC AGATCTTAGC TGTTTGGAAG TAGAGGATGG AGCTGTCTTT 
TTGAAAGAAG AAATAACTGT GGAGCAAAAT TATTCGGAAA CTATGTTTAA GAAGGCAAAA 
ACAAAAGCTA AAAAGAAGCC ACGGAAGCGC TCAGATAGTT CTGGAGGTTA CAACCTTTCA 
GATATTATTC AGACTCCACC ATCTGCAGGA TTATTGAAGT CTGGGAAGAC CAGTTCTGTG 
GAATCTCTTC CAGAACTGTT GACCTCAGAT TCTGAAGGAA GCTACACAGG AGTGGGTAGT 
CCCAGAGACC TGCAGTCCCC TGATTTCACA GCAGGATTTA ATTCAGATAA GATTGAGGGG 
AAAGCTAAAT CGTATGTGAA TGGTACGCCT CCCATGTGTT CTAGGGAAGG GTTCAAACCA 
TGGGAAAAAT CACCAGCATC TACATCTGCT CCCCAGCTCA TCCCCAGTAA CAGAGCTGAC 
ACTCCAGGCT CATCCATCTG GGTTGCGAGC TCTTGCAGTC CTGTGAGCCC TCCTGTTGTG 
GATCTCAGAA CCATCATGGA AATAGAAGAA AGTAGAAAAA AATGTGGCAC AACACCAAAA 
TTGAACTTAG GAAAAATTGT TTCTCATGGA GTTAAACTTT CTCAGAAACA ACGAAAAATG 
ATTGCATTGA CAACAAAAGA CAACCTTTCA GGAACAGGCA ACATGGAAAC AGTTGTTGTA 
GCTCCTTCCA AGCCTCCTAA GCCAGTGAAT GCACGGGCAT CATCTCTACA GTCCCCTTCA 
TCCAAGTCAT TCTGGGATTT CTTACTAGAA GAAAAAAAGT CTATTATTGG CCATAGTTCA 
GGTGATCAAG ATCATGTCAA AAAAGTTTGT TTTAAAGGAA ATGAAAATTC TCAGGCTCTA 
AATGTTGTAA GGTGCTCAAC TCATGGAAGC CCAGGACTAG AAAACAACCT TATTTCTGAT 
TTACCACTTC TAGACAGTCC CAATCCCTGG CTGTCGTCTT CGTTGACTGC TTCTACCACA 
GCAGCTCCGG TCACCTTCGC ATCAATTGTA GAAGAGGAGC TGCAGCAGGA AGCAGCTCTT 
ATCAGAAGTC GGGAAAAACC CTTGGCCCTG ATTCAGGTTG AGGAACATGC CATACAAGAT 
TTGCTGGTTT TCTATGAGGC GCTTGGCAAC CCGGATGAGT TTGTCATCGT GGAAAGGACA 
CCACAGGGGC CACTGGCAGT GCCTATGTGG AACAAACACG GATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008841583.1
CDS	522..4589
Translation	MNSATPDCTPKCRSLQHAADVLSVVTKGSEKQIKTFLASHCYSAATVKDAFGRNAVHLASSCGKKGVLDWLLERGVDPLVKDKESGWTALHRSIFYGHIDCLWSLLKHGVSLYMQDKEGLSPLDLLMKDRPAHVVFHNTDPTEVYTWGDNTNFTLGHGNQNSKHHPELVDLFSRGGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLVPRLVEGLSGHNCSQVAAAKDHTVVLTEDGCVYTFGLNTFHQLGITPPPSSCNVPRQMQAKYLKGRTVIGVAAGRFHTVLWTRDAVYTMGLNGGQLGYLLDPNGEKCVIAPRQVSALHHKDVALSLVAASDGATVCVTTRGDIYLLADYQCKKIASKQLNLEKVLVSGGCMEYKVDPEHLKENGGQKICVLAMDGAGRVFCWRSVHSSLKQCRWAYPRQVLISDIALNRNEVLFVTQDGEGFKGKWFEGKRKNSEKKADILPNLHHSSSDVSCVSDTNSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDEMDNIHDVTFQVGTRTFPAHKYVLAVRSDFFQKLFLSDGTPLEVSDVYRKEEDSAGCHLFVVEKVHPDLFECLLQFMYTDTCDFLTHGFKPRIFINKKSEEYQGSLNSLMNTMNCPEDETQKSAFEVYKSSQAHTVSEKQKGKPKSSKKGRSASEDDPVKMLQNVAKKFGLSNLSGRLDGVRFENEKINVIAKKTGNKLKLSQKKCSFLCDVIMKSVDGKEYPCHKCVLCARLEYFHSMLSSSWIEASSGAALEMPVRSEVLRVILDYLYTDEALLIKESQNVDFICSVLVVADQLLITRLKEICEVALTEKLTLKNAAMLLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDDVLKDLSIFYRKMIPAMDRRIITPYQDGPDLSCLEVEDGAVFLKEEITVEQNYSETMFKKAKTKAKKKPRKRSDSSGGYNLSDIIQTPPSAGLLKSGKTSSVESLPELLTSDSEGSYTGVGSPRDLQSPDFTAGFNSDKIEGKAKSYVNGTPPMCSREGFKPWEKSPASTSAPQLIPSNRADTPGSSIWVASSCSPVSPPVVDLRTIMEIEESRKKCGTTPKLNLGKIVSHGVKLSQKQRKMIALTTKDNLSGTGNMETVVVAPSKPPKPVNARASSLQSPSSKSFWDFLLEEKKSIIGHSSGDQDHVKKVCFKGNENSQALNVVRCSTHGSPGLENNLISDLPLLDSPNPWLSSSLTASTTAAPVTFASIVEEELQQEAALIRSREKPLALIQVEEHAIQDLLVFYEALGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_008843361.3
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008843361.3

ORF Insert Sequence:

ATGAACTCTG CCACCCCCGA CTGCACCCCC AAATGCCGAT CCCTGCAACA CGCTGCAGAC 
GTCCTTTCCG TGGTAACCAA GGGGAGTGAA AAGCAGATTA AGACCTTCCT CGCCAGCCAC 
TGCTACAGTG CTGCGACTGT CAAAGATGCC TTTGGCAGGA ATGCTGTCCA CCTGGCCTCC 
TCCTGTGGGA AGAAAGGCGT GCTAGACTGG CTTTTGGAGA GGGGAGTGGA TCCACTTGTG 
AAAGACAAGG AGTCAGGCTG GACTGCGCTG CACAGGAGCA TTTTCTATGG ACATATTGAT 
TGTCTTTGGT CTCTGTTGAA GCATGGTGTT AGTCTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTAAT GAAGGATAGA CCAGCTCATG TAGTATTTCA CAACACTGAC 
CCCACAGAAG TCTACACTTG GGGAGATAAT ACAAACTTTA CTCTGGGTCA TGGAAACCAG 
AACAGCAAAC ATCACCCAGA ACTGGTGGAT TTGTTCTCCA GGGGTGGCGT TTATATCAAA 
CAGGTGGTGC TTTGCAAATT TCATTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC 
TGTGGGCATG GTCCAGGAGG AAGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGACTTGTGG AAGGACTGAG TGGTCATAAC TGTTCCCAGG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACCTTTG GTCTCAATAC TTTTCACCAA 
TTAGGAATTA CTCCTCCTCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC 
CTGAAAGGAA GAACTGTCAT TGGAGTAGCA GCAGGCAGGT TTCACACAGT TCTGTGGACT 
CGAGATGCCG TTTACACTAT GGGCCTGAAT GGTGGACAGC TGGGTTACTT ACTGGATCCC 
AATGGAGAAA AGTGTGTCAT AGCTCCTCGC CAGGTCTCTG CCCTTCATCA TAAGGATGTT 
GCTCTGTCTT TGGTTGCTGC AAGTGATGGT GCCACAGTCT GTGTTACCAC AAGAGGAGAT 
ATATACTTAC TTGCAGACTA TCAGTGCAAG AAGATAGCAT CTAAACAACT GAACTTGGAA 
AAGGTTCTTG TATCTGGGGG TTGTATGGAA TACAAAGTCG ATCCTGAACA TTTGAAAGAA 
AATGGGGGTC AGAAAATCTG CGTTCTTGCT ATGGATGGAG CCGGAAGAGT ATTTTGCTGG 
AGATCAGTCC ACAGTTCTCT GAAGCAATGC CGATGGGCCT ATCCTCGCCA GGTGTTAATT 
TCTGATATTG CTTTAAATAG AAATGAAGTT CTGTTTGTCA CCCAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGG AAAAAGAAAG AATTCTGAAA AGAAAGCAGA CATTTTACCA 
AACCTACATC ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATACAAATAG TGTGTATGAA 
AGAATTCGAC TAGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGACCCA 
AGTGGATGCA ACTTTGCAAT CCTACAGTCA GACCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCTGTGTCTT CATCATCCTT TTTTGAAGAA TTTGGCAAAC TCTTGAGGGA GACAGATGAA 
ATGGACAACA TTCACGATGT GACATTTCAA GTTGGCACTA GAACCTTCCC TGCTCATAAA 
TATGTTTTGG CTGTGCGTTC TGACTTTTTC CAGAAATTGT TTCTTTCAGA TGGTACTCCC 
TTGGAAGTCT CAGATGTGTA CCGGAAAGAG GAAGATTCTG CAGGGTGCCA TCTCTTTGTG 
GTGGAGAAGG TTCATCCTGA TTTGTTTGAA TGCCTTTTAC AATTCATGTA TACAGATACT 
TGTGACTTTT TAACTCATGG TTTTAAGCCA AGGATATTCA TAAACAAAAA GTCAGAAGAA 
TATCAGGGCT CCCTGAATTC TCTTATGAAT ACGATGAATT GCCCTGAGGA TGAGACCCAG 
AAGTCGGCGT TTGAGGTCTA CAAAAGTAGC CAAGCTCACA CAGTTAGTGA AAAGCAGAAA 
GGCAAACCCA AATCTTCTAA AAAGGGAAGA AGTGCTAGTG AAGATGATCC TGTCAAGATG 
TTGCAAAATG TTGCAAAGAA ATTTGGCCTC AGTAATCTGA GTGGCAGGTT AGATGGAGTC 
AGATTTGAAA ATGAAAAAAT TAATGTTATT GCAAAGAAAA CTGGCAATAA ATTGAAGCTA 
AGTCAAAAAA AATGTTCATT TCTGTGTGAT GTTATCATGA AATCAGTGGA TGGAAAGGAA 
TATCCTTGTC ATAAATGTGT CCTTTGTGCT AGACTTGAGT ATTTTCATAG TATGCTGAGT 
AGCTCGTGGA TTGAGGCTTC GAGTGGTGCC GCTCTGGAAA TGCCAGTACG TTCTGAGGTC 
CTGAGAGTGA TTTTGGACTA CCTCTACACT GATGAAGCTC TGCTGATAAA AGAATCTCAA 
AATGTGGATT TTATTTGTAG TGTTCTTGTA GTGGCTGATC AGCTTCTGAT AACTCGATTA 
AAGGAGATTT GTGAAGTGGC ATTAACTGAA AAACTTACCC TTAAGAATGC TGCTATGCTA 
CTGGAATTTG CAGCAATGTA CAATGCTGAA CAGTTGAAAC TGTCTTGTTT ACAGTTTATA 
GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGGTCTCTTG ATGTTTTAAG TGATGATGTT 
TTGAAGGATC TTTCCATATT TTACCGAAAA ATGATCCCAG CAATGGATAG AAGAATCATT 
ACACCATACC AAGATGGACC AGATCTTAGC TGTTTGGAAG TAGAGGATGG AGCTGTCTTT 
TTGAAAGAAG AAATAACTGT GGAGCAAAAT TATTCGGAAA CTATGTTTAA GAAGGCAAAA 
ACAAAAGCTA AAAAGAAGCC ACGGAAGCGC TCAGATAGTT CTGGAGGTTA CAACCTTTCA 
GATATTATTC AGACTCCACC ATCTGCAGGA TTATTGAAGT CTGGGAAGAC CAGTTCTGTG 
GAATCTCTTC CAGAACTGTT GACCTCAGAT TCTGAAGGAA GCTACACAGG AGTGGGTAGT 
CCCAGAGACC TGCAGTCCCC TGATTTCACA GCAGGATTTA ATTCAGATAA GATTGAGGGG 
AAAGCTAAAT CGTATGTGAA TGGTACGCCT CCCATGTGTT CTAGGGAAGG GTTCAAACCA 
TGGGAAAAAT CACCAGCATC TACATCTGCT CCCCAGCTCA TCCCCAGTAA CAGAGCTGAC 
ACTCCAGGCT CATCCATCTG GGTTGCGAGC TCTTGCAGTC CTGTGAGCCC TCCTGTTGTG 
GATCTCAGAA CCATCATGGA AATAGAAGAA AGTAGAAAAA AATGTGGCAC AACACCAAAA 
TTGAACTTAG GAAAAATTGT TTCTCATGGA GTTAAACTTT CTCAGAAACA ACGAAAAATG 
ATTGCATTGA CAACAAAAGA CAACCTTTCA GGAACAGGCA ACATGGAAAC AGTTGTTGTA 
GCTCCTTCCA AGCCTCCTAA GCCAGTGAAT GCACGGGCAT CATCTCTACA GTCCCCTTCA 
TCCAAGTCAT TCTGGGATTT CTTACTAGAA GAAAAAAAGT CTATTATTGG CCATAGTTCA 
GGTGATCAAG ATCATGTCAA AAAAGTTTGT TTTAAAGGAA ATGAAAATTC TCAGGCTCTA 
AATGTTGTAA GGTGCTCAAC TCATGGAAGC CCAGGACTAG AAAACAACCT TATTTCTGAT 
TTACCACTTC TAGACAGTCC CAATCCCTGG CTGTCGTCTT CGTTGACTGC TTCTACCACA 
GCAGCTCCGG TCACCTTCGC ATCAATTGTA GAAGAGGAGC TGCAGCAGGA AGCAGCTCTT 
ATCAGAAGTC GGGAAAAACC CTTGGCCCTG ATTCAGGTTG AGGAACATGC CATACAAGAT 
TTGCTGGTTT TCTATGAGGC GCTTGGCAAC CCGGATGAGT TTGTCATCGT GGAAAGGACA 
CCACAGGGGC CACTGGCAGT GCCTATGTGG AACAAACACG GATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ibtk ( XM_017800682.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017656171.1 Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONa25777
Clone ID Related Accession (Same CDS sequence)	XM_017800682.1 , XM_017800682.2
Accession Version	XM_017800682.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	inhibitor of Bruton tyrosine kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008346901.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTG CCACCCCCGA CTGCACCCCC AAATGCCGAT CCCTGCAACA CGCTGCAGAC 
GTCCTTTCCG TGGTAACCAA GGGGAGTGAA AAGCAGATTA AGACCTTCCT CGCCAGCCAC 
TGCTACAGTG CTGCGACTGT CAAAGATGCC TTTGGCAGGA ATGCTGTCCA CCTGGCCTCC 
TCCTGTGGGA AGAAAGGCGT GCTAGACTGG CTTTTGGAGA GGGGAGTGGA TCCACTTGTG 
AAAGACAAGG AGTCAGGCTG GACTGCGCTG CACAGGAGCA TTTTCTATGG ACATATTGAT 
TGTCTTTGGT CTCTGTTGAA GCATGGTGTT AGTCTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTAAT GAAGGATAGA CCAGCTCATG TAGTATTTCA CAACACTGAC 
CCCACAGAAG TCTACACTTG GGGAGATAAT ACAAACTTTA CTCTGGGTCA TGGAAACCAG 
AACAGCAAAC ATCACCCAGA ACTGGTGGAT TTGTTCTCCA GGGGTGGCGT TTATATCAAA 
CAGGTGGTGC TTTGCAAATT TCATTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC 
TGTGGGCATG GTCCAGGAGG AAGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGACTTGTGG AAGGACTGAG TGGTCATAAC TGTTCCCAGG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACCTTTG GTCTCAATAC TTTTCACCAA 
TTAGGAATTA CTCCTCCTCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC 
CTGAAAGGAA GAACTGTCAT TGGAGTAGCA GCAGGCAGGT TTCACACAGT TCTGTGGACT 
CGAGATGCCG TTTACACTAT GGGCCTGAAT GGTGGACAGC TGGGTTACTT ACTGGATCCC 
AATGGAGAAA AGTGTGTCAT AGCTCCTCGC CAGGTCTCTG CCCTTCATCA TAAGGATGTT 
GCTCTGTCTT TGGTTGCTGC AAGTGATGGT GCCACAGTCT GTGTTACCAC AAGAGGAGAT 
ATATACTTAC TTGCAGACTA TCAGTGCAAG AAGATAGCAT CTAAACAACT GAACTTGGAA 
AAGGTTCTTG TATCTGGGGG TTGTATGGAA TACAAAGTCG ATCCTGAACA TTTGAAAGAA 
AATGGGGGTC AGAAAATCTG CGTTCTTGCT ATGGATGGAG CCGGAAGAGT ATTTTGCTGG 
AGATCAGTCC ACAGTTCTCT GAAGCAATGC CGATGGGCCT ATCCTCGCCA GGTGTTAATT 
TCTGATATTG CTTTAAATAG AAATGAAGTT CTGTTTGTCA CCCAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGG AAAAAGAAAG AATTCTGAAA AGAAAGACAT TTTACCAAAC 
CTACATCATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA CAAATAGTGT GTATGAAAGA 
ATTCGACTAG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGACCCAAGT 
GGATGCAACT TTGCAATCCT ACAGTCAGAC CCTAAAACAA GCCTTTATGA AATTCCAGCT 
GTGTCTTCAT CATCCTTTTT TGAAGAATTT GGCAAACTCT TGAGGGAGAC AGATGAAATG 
GACAACATTC ACGATGTGAC ATTTCAAGTT GGCACTAGAA CCTTCCCTGC TCATAAATAT 
GTTTTGGCTG TGCGTTCTGA CTTTTTCCAG AAATTGTTTC TTTCAGATGG TACTCCCTTG 
GAAGTCTCAG ATGTGTACCG GAAAGAGGAA GATTCTGCAG GGTGCCATCT CTTTGTGGTG 
GAGAAGGTTC ATCCTGATTT GTTTGAATGC CTTTTACAAT TCATGTATAC AGATACTTGT 
GACTTTTTAA CTCATGGTTT TAAGCCAAGG ATATTCATAA ACAAAAAGTC AGAAGAATAT 
CAGGGCTCCC TGAATTCTCT TATGAATACG ATGAATTGCC CTGAGGATGA GACCCAGAAG 
TCGGCGTTTG AGGTCTACAA AAGTAGCCAA GCTCACACAG TTAGTGAAAA GCAGAAAGGC 
AAACCCAAAT CTTCTAAAAA GGGAAGAAGT GCTAGTGAAG ATGATCCTGT CAAGATGTTG 
CAAAATGTTG CAAAGAAATT TGGCCTCAGT AATCTGAGTG GCAGGTTAGA TGGAGTCAGA 
TTTGAAAATG AAAAAATTAA TGTTATTGCA AAGAAAACTG GCAATAAATT GAAGCTAAGT 
CAAAAAAAAT GTTCATTTCT GTGTGATGTT ATCATGAAAT CAGTGGATGG AAAGGAATAT 
CCTTGTCATA AATGTGTCCT TTGTGCTAGA CTTGAGTATT TTCATAGTAT GCTGAGTAGC 
TCGTGGATTG AGGCTTCGAG TGGTGCCGCT CTGGAAATGC CAGTACGTTC TGAGGTCCTG 
AGAGTGATTT TGGACTACCT CTACACTGAT GAAGCTCTGC TGATAAAAGA ATCTCAAAAT 
GTGGATTTTA TTTGTAGTGT TCTTGTAGTG GCTGATCAGC TTCTGATAAC TCGATTAAAG 
GAGATTTGTG AAGTGGCATT AACTGAAAAA CTTACCCTTA AGAATGCTGC TATGCTACTG 
GAATTTGCAG CAATGTACAA TGCTGAACAG TTGAAACTGT CTTGTTTACA GTTTATAGGA 
CTGAATATGG CAGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGATGTTTTG 
AAGGATCTTT CCATATTTTA CCGAAAAATG ATCCCAGCAA TGGATAGAAG AATCATTACA 
CCATACCAAG ATGGACCAGA TCTTAGCTGT TTGGAAGTAG AGGATGGAGC TGTCTTTTTG 
AAAGAAGAAA TAACTGTGGA GCAAAATTAT TCGGAAACTA TGTTTAAGAA GGCAAAAACA 
AAAGCTAAAA AGAAGCCACG GAAGCGCTCA GATAGTTCTG GAGGTTACAA CCTTTCAGAT 
ATTATTCAGA CTCCACCATC TGCAGGATTA TTGAAGTCTG GGAAGACCAG TTCTGTGGAA 
TCTCTTCCAG AACTGTTGAC CTCAGATTCT GAAGGAAGCT ACACAGGAGT GGGTAGTCCC 
AGAGACCTGC AGTCCCCTGA TTTCACAGCA GGATTTAATT CAGATAAGAT TGAGGGGAAA 
GCTAAATCGT ATGTGAATGG TACGCCTCCC ATGTGTTCTA GGGAAGGGTT CAAACCATGG 
GAAAAATCAC CAGCATCTAC ATCTGCTCCC CAGCTCATCC CCAGTAACAG AGCTGACACT 
CCAGGCTCAT CCATCTGGGT TGCGAGCTCT TGCAGTCCTG TGAGCCCTCC TGTTGTGGAT 
CTCAGAACCA TCATGGAAAT AGAAGAAAGT AGAAAAAAAT GTGGCACAAC ACCAAAATTG 
AACTTAGGAA AAATTGTTTC TCATGGAGTT AAACTTTCTC AGAAACAACG AAAAATGATT 
GCATTGACAA CAAAAGACAA CCTTTCAGGA ACAGGCAACA TGGAAACAGT TGTTGTAGCT 
CCTTCCAAGC CTCCTAAGCC AGTGAATGCA CGGGCATCAT CTCTACAGTC CCCTTCATCC 
AAGTCATTCT GGGATTTCTT ACTAGAAGAA AAAAAGTCTA TTATTGGCCA TAGTTCAGGT 
GATCAAGATC ATGTCAAAAA AGTTTGTTTT AAAGGAAATG AAAATTCTCA GGCTCTAAAT 
GTTGTAAGGT GCTCAACTCA TGGAAGCCCA GGACTAGAAA ACAACCTTAT TTCTGATTTA 
CCACTTCTAG ACAGTCCCAA TCCCTGGCTG TCGTCTTCGT TGACTGCTTC TACCACAGCA 
GCTCCGGTCA CCTTCGCATC AATTGTAGAA GAGGAGCTGC AGCAGGAAGC AGCTCTTATC 
AGAAGTCGGG AAAAACCCTT GGCCCTGATT CAGGTTGAGG AACATGCCAT ACAAGATTTG 
CTGGTTTTCT ATGAGGCGCT TGGCAACCCG GATGAGTTTG TCATCGTGGA AAGGACACCA 
CAGGGGCCAC TGGCAGTGCC TATGTGGAAC AAACACGGAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017656171.1
CDS	523..4587
Translation	MNSATPDCTPKCRSLQHAADVLSVVTKGSEKQIKTFLASHCYSAATVKDAFGRNAVHLASSCGKKGVLDWLLERGVDPLVKDKESGWTALHRSIFYGHIDCLWSLLKHGVSLYMQDKEGLSPLDLLMKDRPAHVVFHNTDPTEVYTWGDNTNFTLGHGNQNSKHHPELVDLFSRGGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLVPRLVEGLSGHNCSQVAAAKDHTVVLTEDGCVYTFGLNTFHQLGITPPPSSCNVPRQMQAKYLKGRTVIGVAAGRFHTVLWTRDAVYTMGLNGGQLGYLLDPNGEKCVIAPRQVSALHHKDVALSLVAASDGATVCVTTRGDIYLLADYQCKKIASKQLNLEKVLVSGGCMEYKVDPEHLKENGGQKICVLAMDGAGRVFCWRSVHSSLKQCRWAYPRQVLISDIALNRNEVLFVTQDGEGFKGKWFEGKRKNSEKKDILPNLHHSSSDVSCVSDTNSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDEMDNIHDVTFQVGTRTFPAHKYVLAVRSDFFQKLFLSDGTPLEVSDVYRKEEDSAGCHLFVVEKVHPDLFECLLQFMYTDTCDFLTHGFKPRIFINKKSEEYQGSLNSLMNTMNCPEDETQKSAFEVYKSSQAHTVSEKQKGKPKSSKKGRSASEDDPVKMLQNVAKKFGLSNLSGRLDGVRFENEKINVIAKKTGNKLKLSQKKCSFLCDVIMKSVDGKEYPCHKCVLCARLEYFHSMLSSSWIEASSGAALEMPVRSEVLRVILDYLYTDEALLIKESQNVDFICSVLVVADQLLITRLKEICEVALTEKLTLKNAAMLLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDDVLKDLSIFYRKMIPAMDRRIITPYQDGPDLSCLEVEDGAVFLKEEITVEQNYSETMFKKAKTKAKKKPRKRSDSSGGYNLSDIIQTPPSAGLLKSGKTSSVESLPELLTSDSEGSYTGVGSPRDLQSPDFTAGFNSDKIEGKAKSYVNGTPPMCSREGFKPWEKSPASTSAPQLIPSNRADTPGSSIWVASSCSPVSPPVVDLRTIMEIEESRKKCGTTPKLNLGKIVSHGVKLSQKQRKMIALTTKDNLSGTGNMETVVVAPSKPPKPVNARASSLQSPSSKSFWDFLLEEKKSIIGHSSGDQDHVKKVCFKGNENSQALNVVRCSTHGSPGLENNLISDLPLLDSPNPWLSSSLTASTTAAPVTFASIVEEELQQEAALIRSREKPLALIQVEEHAIQDLLVFYEALGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_017800682.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017800682.1

ORF Insert Sequence:

ATGAACTCTG CCACCCCCGA CTGCACCCCC AAATGCCGAT CCCTGCAACA CGCTGCAGAC 
GTCCTTTCCG TGGTAACCAA GGGGAGTGAA AAGCAGATTA AGACCTTCCT CGCCAGCCAC 
TGCTACAGTG CTGCGACTGT CAAAGATGCC TTTGGCAGGA ATGCTGTCCA CCTGGCCTCC 
TCCTGTGGGA AGAAAGGCGT GCTAGACTGG CTTTTGGAGA GGGGAGTGGA TCCACTTGTG 
AAAGACAAGG AGTCAGGCTG GACTGCGCTG CACAGGAGCA TTTTCTATGG ACATATTGAT 
TGTCTTTGGT CTCTGTTGAA GCATGGTGTT AGTCTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTAAT GAAGGATAGA CCAGCTCATG TAGTATTTCA CAACACTGAC 
CCCACAGAAG TCTACACTTG GGGAGATAAT ACAAACTTTA CTCTGGGTCA TGGAAACCAG 
AACAGCAAAC ATCACCCAGA ACTGGTGGAT TTGTTCTCCA GGGGTGGCGT TTATATCAAA 
CAGGTGGTGC TTTGCAAATT TCATTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC 
TGTGGGCATG GTCCAGGAGG AAGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGACTTGTGG AAGGACTGAG TGGTCATAAC TGTTCCCAGG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACCTTTG GTCTCAATAC TTTTCACCAA 
TTAGGAATTA CTCCTCCTCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC 
CTGAAAGGAA GAACTGTCAT TGGAGTAGCA GCAGGCAGGT TTCACACAGT TCTGTGGACT 
CGAGATGCCG TTTACACTAT GGGCCTGAAT GGTGGACAGC TGGGTTACTT ACTGGATCCC 
AATGGAGAAA AGTGTGTCAT AGCTCCTCGC CAGGTCTCTG CCCTTCATCA TAAGGATGTT 
GCTCTGTCTT TGGTTGCTGC AAGTGATGGT GCCACAGTCT GTGTTACCAC AAGAGGAGAT 
ATATACTTAC TTGCAGACTA TCAGTGCAAG AAGATAGCAT CTAAACAACT GAACTTGGAA 
AAGGTTCTTG TATCTGGGGG TTGTATGGAA TACAAAGTCG ATCCTGAACA TTTGAAAGAA 
AATGGGGGTC AGAAAATCTG CGTTCTTGCT ATGGATGGAG CCGGAAGAGT ATTTTGCTGG 
AGATCAGTCC ACAGTTCTCT GAAGCAATGC CGATGGGCCT ATCCTCGCCA GGTGTTAATT 
TCTGATATTG CTTTAAATAG AAATGAAGTT CTGTTTGTCA CCCAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGG AAAAAGAAAG AATTCTGAAA AGAAAGACAT TTTACCAAAC 
CTACATCATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA CAAATAGTGT GTATGAAAGA 
ATTCGACTAG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGACCCAAGT 
GGATGCAACT TTGCAATCCT ACAGTCAGAC CCTAAAACAA GCCTTTATGA AATTCCAGCT 
GTGTCTTCAT CATCCTTTTT TGAAGAATTT GGCAAACTCT TGAGGGAGAC AGATGAAATG 
GACAACATTC ACGATGTGAC ATTTCAAGTT GGCACTAGAA CCTTCCCTGC TCATAAATAT 
GTTTTGGCTG TGCGTTCTGA CTTTTTCCAG AAATTGTTTC TTTCAGATGG TACTCCCTTG 
GAAGTCTCAG ATGTGTACCG GAAAGAGGAA GATTCTGCAG GGTGCCATCT CTTTGTGGTG 
GAGAAGGTTC ATCCTGATTT GTTTGAATGC CTTTTACAAT TCATGTATAC AGATACTTGT 
GACTTTTTAA CTCATGGTTT TAAGCCAAGG ATATTCATAA ACAAAAAGTC AGAAGAATAT 
CAGGGCTCCC TGAATTCTCT TATGAATACG ATGAATTGCC CTGAGGATGA GACCCAGAAG 
TCGGCGTTTG AGGTCTACAA AAGTAGCCAA GCTCACACAG TTAGTGAAAA GCAGAAAGGC 
AAACCCAAAT CTTCTAAAAA GGGAAGAAGT GCTAGTGAAG ATGATCCTGT CAAGATGTTG 
CAAAATGTTG CAAAGAAATT TGGCCTCAGT AATCTGAGTG GCAGGTTAGA TGGAGTCAGA 
TTTGAAAATG AAAAAATTAA TGTTATTGCA AAGAAAACTG GCAATAAATT GAAGCTAAGT 
CAAAAAAAAT GTTCATTTCT GTGTGATGTT ATCATGAAAT CAGTGGATGG AAAGGAATAT 
CCTTGTCATA AATGTGTCCT TTGTGCTAGA CTTGAGTATT TTCATAGTAT GCTGAGTAGC 
TCGTGGATTG AGGCTTCGAG TGGTGCCGCT CTGGAAATGC CAGTACGTTC TGAGGTCCTG 
AGAGTGATTT TGGACTACCT CTACACTGAT GAAGCTCTGC TGATAAAAGA ATCTCAAAAT 
GTGGATTTTA TTTGTAGTGT TCTTGTAGTG GCTGATCAGC TTCTGATAAC TCGATTAAAG 
GAGATTTGTG AAGTGGCATT AACTGAAAAA CTTACCCTTA AGAATGCTGC TATGCTACTG 
GAATTTGCAG CAATGTACAA TGCTGAACAG TTGAAACTGT CTTGTTTACA GTTTATAGGA 
CTGAATATGG CAGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGATGTTTTG 
AAGGATCTTT CCATATTTTA CCGAAAAATG ATCCCAGCAA TGGATAGAAG AATCATTACA 
CCATACCAAG ATGGACCAGA TCTTAGCTGT TTGGAAGTAG AGGATGGAGC TGTCTTTTTG 
AAAGAAGAAA TAACTGTGGA GCAAAATTAT TCGGAAACTA TGTTTAAGAA GGCAAAAACA 
AAAGCTAAAA AGAAGCCACG GAAGCGCTCA GATAGTTCTG GAGGTTACAA CCTTTCAGAT 
ATTATTCAGA CTCCACCATC TGCAGGATTA TTGAAGTCTG GGAAGACCAG TTCTGTGGAA 
TCTCTTCCAG AACTGTTGAC CTCAGATTCT GAAGGAAGCT ACACAGGAGT GGGTAGTCCC 
AGAGACCTGC AGTCCCCTGA TTTCACAGCA GGATTTAATT CAGATAAGAT TGAGGGGAAA 
GCTAAATCGT ATGTGAATGG TACGCCTCCC ATGTGTTCTA GGGAAGGGTT CAAACCATGG 
GAAAAATCAC CAGCATCTAC ATCTGCTCCC CAGCTCATCC CCAGTAACAG AGCTGACACT 
CCAGGCTCAT CCATCTGGGT TGCGAGCTCT TGCAGTCCTG TGAGCCCTCC TGTTGTGGAT 
CTCAGAACCA TCATGGAAAT AGAAGAAAGT AGAAAAAAAT GTGGCACAAC ACCAAAATTG 
AACTTAGGAA AAATTGTTTC TCATGGAGTT AAACTTTCTC AGAAACAACG AAAAATGATT 
GCATTGACAA CAAAAGACAA CCTTTCAGGA ACAGGCAACA TGGAAACAGT TGTTGTAGCT 
CCTTCCAAGC CTCCTAAGCC AGTGAATGCA CGGGCATCAT CTCTACAGTC CCCTTCATCC 
AAGTCATTCT GGGATTTCTT ACTAGAAGAA AAAAAGTCTA TTATTGGCCA TAGTTCAGGT 
GATCAAGATC ATGTCAAAAA AGTTTGTTTT AAAGGAAATG AAAATTCTCA GGCTCTAAAT 
GTTGTAAGGT GCTCAACTCA TGGAAGCCCA GGACTAGAAA ACAACCTTAT TTCTGATTTA 
CCACTTCTAG ACAGTCCCAA TCCCTGGCTG TCGTCTTCGT TGACTGCTTC TACCACAGCA 
GCTCCGGTCA CCTTCGCATC AATTGTAGAA GAGGAGCTGC AGCAGGAAGC AGCTCTTATC 
AGAAGTCGGG AAAAACCCTT GGCCCTGATT CAGGTTGAGG AACATGCCAT ACAAGATTTG 
CTGGTTTTCT ATGAGGCGCT TGGCAACCCG GATGAGTTTG TCATCGTGGA AAGGACACCA 
CAGGGGCCAC TGGCAGTGCC TATGTGGAAC AAACACGGAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ibtk ( XM_017800682.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017656171.1 Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONa25777
Clone ID Related Accession (Same CDS sequence)	XM_017800682.1 , XM_017800682.2
Accession Version	XM_017800682.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	inhibitor of Bruton tyrosine kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008346901.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_017800682.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTG CCACCCCCGA CTGCACCCCC AAATGCCGAT CCCTGCAACA CGCTGCAGAC 
GTCCTTTCCG TGGTAACCAA GGGGAGTGAA AAGCAGATTA AGACCTTCCT CGCCAGCCAC 
TGCTACAGTG CTGCGACTGT CAAAGATGCC TTTGGCAGGA ATGCTGTCCA CCTGGCCTCC 
TCCTGTGGGA AGAAAGGCGT GCTAGACTGG CTTTTGGAGA GGGGAGTGGA TCCACTTGTG 
AAAGACAAGG AGTCAGGCTG GACTGCGCTG CACAGGAGCA TTTTCTATGG ACATATTGAT 
TGTCTTTGGT CTCTGTTGAA GCATGGTGTT AGTCTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTAAT GAAGGATAGA CCAGCTCATG TAGTATTTCA CAACACTGAC 
CCCACAGAAG TCTACACTTG GGGAGATAAT ACAAACTTTA CTCTGGGTCA TGGAAACCAG 
AACAGCAAAC ATCACCCAGA ACTGGTGGAT TTGTTCTCCA GGGGTGGCGT TTATATCAAA 
CAGGTGGTGC TTTGCAAATT TCATTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC 
TGTGGGCATG GTCCAGGAGG AAGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGACTTGTGG AAGGACTGAG TGGTCATAAC TGTTCCCAGG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACCTTTG GTCTCAATAC TTTTCACCAA 
TTAGGAATTA CTCCTCCTCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC 
CTGAAAGGAA GAACTGTCAT TGGAGTAGCA GCAGGCAGGT TTCACACAGT TCTGTGGACT 
CGAGATGCCG TTTACACTAT GGGCCTGAAT GGTGGACAGC TGGGTTACTT ACTGGATCCC 
AATGGAGAAA AGTGTGTCAT AGCTCCTCGC CAGGTCTCTG CCCTTCATCA TAAGGATGTT 
GCTCTGTCTT TGGTTGCTGC AAGTGATGGT GCCACAGTCT GTGTTACCAC AAGAGGAGAT 
ATATACTTAC TTGCAGACTA TCAGTGCAAG AAGATAGCAT CTAAACAACT GAACTTGGAA 
AAGGTTCTTG TATCTGGGGG TTGTATGGAA TACAAAGTCG ATCCTGAACA TTTGAAAGAA 
AATGGGGGTC AGAAAATCTG CGTTCTTGCT ATGGATGGAG CCGGAAGAGT ATTTTGCTGG 
AGATCAGTCC ACAGTTCTCT GAAGCAATGC CGATGGGCCT ATCCTCGCCA GGTGTTAATT 
TCTGATATTG CTTTAAATAG AAATGAAGTT CTGTTTGTCA CCCAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGG AAAAAGAAAG AATTCTGAAA AGAAAGACAT TTTACCAAAC 
CTACATCATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA CAAATAGTGT GTATGAAAGA 
ATTCGACTAG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGACCCAAGT 
GGATGCAACT TTGCAATCCT ACAGTCAGAC CCTAAAACAA GCCTTTATGA AATTCCAGCT 
GTGTCTTCAT CATCCTTTTT TGAAGAATTT GGCAAACTCT TGAGGGAGAC AGATGAAATG 
GACAACATTC ACGATGTGAC ATTTCAAGTT GGCACTAGAA CCTTCCCTGC TCATAAATAT 
GTTTTGGCTG TGCGTTCTGA CTTTTTCCAG AAATTGTTTC TTTCAGATGG TACTCCCTTG 
GAAGTCTCAG ATGTGTACCG GAAAGAGGAA GATTCTGCAG GGTGCCATCT CTTTGTGGTG 
GAGAAGGTTC ATCCTGATTT GTTTGAATGC CTTTTACAAT TCATGTATAC AGATACTTGT 
GACTTTTTAA CTCATGGTTT TAAGCCAAGG ATATTCATAA ACAAAAAGTC AGAAGAATAT 
CAGGGCTCCC TGAATTCTCT TATGAATACG ATGAATTGCC CTGAGGATGA GACCCAGAAG 
TCGGCGTTTG AGGTCTACAA AAGTAGCCAA GCTCACACAG TTAGTGAAAA GCAGAAAGGC 
AAACCCAAAT CTTCTAAAAA GGGAAGAAGT GCTAGTGAAG ATGATCCTGT CAAGATGTTG 
CAAAATGTTG CAAAGAAATT TGGCCTCAGT AATCTGAGTG GCAGGTTAGA TGGAGTCAGA 
TTTGAAAATG AAAAAATTAA TGTTATTGCA AAGAAAACTG GCAATAAATT GAAGCTAAGT 
CAAAAAAAAT GTTCATTTCT GTGTGATGTT ATCATGAAAT CAGTGGATGG AAAGGAATAT 
CCTTGTCATA AATGTGTCCT TTGTGCTAGA CTTGAGTATT TTCATAGTAT GCTGAGTAGC 
TCGTGGATTG AGGCTTCGAG TGGTGCCGCT CTGGAAATGC CAGTACGTTC TGAGGTCCTG 
AGAGTGATTT TGGACTACCT CTACACTGAT GAAGCTCTGC TGATAAAAGA ATCTCAAAAT 
GTGGATTTTA TTTGTAGTGT TCTTGTAGTG GCTGATCAGC TTCTGATAAC TCGATTAAAG 
GAGATTTGTG AAGTGGCATT AACTGAAAAA CTTACCCTTA AGAATGCTGC TATGCTACTG 
GAATTTGCAG CAATGTACAA TGCTGAACAG TTGAAACTGT CTTGTTTACA GTTTATAGGA 
CTGAATATGG CAGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGATGTTTTG 
AAGGATCTTT CCATATTTTA CCGAAAAATG ATCCCAGCAA TGGATAGAAG AATCATTACA 
CCATACCAAG ATGGACCAGA TCTTAGCTGT TTGGAAGTAG AGGATGGAGC TGTCTTTTTG 
AAAGAAGAAA TAACTGTGGA GCAAAATTAT TCGGAAACTA TGTTTAAGAA GGCAAAAACA 
AAAGCTAAAA AGAAGCCACG GAAGCGCTCA GATAGTTCTG GAGGTTACAA CCTTTCAGAT 
ATTATTCAGA CTCCACCATC TGCAGGATTA TTGAAGTCTG GGAAGACCAG TTCTGTGGAA 
TCTCTTCCAG AACTGTTGAC CTCAGATTCT GAAGGAAGCT ACACAGGAGT GGGTAGTCCC 
AGAGACCTGC AGTCCCCTGA TTTCACAGCA GGATTTAATT CAGATAAGAT TGAGGGGAAA 
GCTAAATCGT ATGTGAATGG TACGCCTCCC ATGTGTTCTA GGGAAGGGTT CAAACCATGG 
GAAAAATCAC CAGCATCTAC ATCTGCTCCC CAGCTCATCC CCAGTAACAG AGCTGACACT 
CCAGGCTCAT CCATCTGGGT TGCGAGCTCT TGCAGTCCTG TGAGCCCTCC TGTTGTGGAT 
CTCAGAACCA TCATGGAAAT AGAAGAAAGT AGAAAAAAAT GTGGCACAAC ACCAAAATTG 
AACTTAGGAA AAATTGTTTC TCATGGAGTT AAACTTTCTC AGAAACAACG AAAAATGATT 
GCATTGACAA CAAAAGACAA CCTTTCAGGA ACAGGCAACA TGGAAACAGT TGTTGTAGCT 
CCTTCCAAGC CTCCTAAGCC AGTGAATGCA CGGGCATCAT CTCTACAGTC CCCTTCATCC 
AAGTCATTCT GGGATTTCTT ACTAGAAGAA AAAAAGTCTA TTATTGGCCA TAGTTCAGGT 
GATCAAGATC ATGTCAAAAA AGTTTGTTTT AAAGGAAATG AAAATTCTCA GGCTCTAAAT 
GTTGTAAGGT GCTCAACTCA TGGAAGCCCA GGACTAGAAA ACAACCTTAT TTCTGATTTA 
CCACTTCTAG ACAGTCCCAA TCCCTGGCTG TCGTCTTCGT TGACTGCTTC TACCACAGCA 
GCTCCGGTCA CCTTCGCATC AATTGTAGAA GAGGAGCTGC AGCAGGAAGC AGCTCTTATC 
AGAAGTCGGG AAAAACCCTT GGCCCTGATT CAGGTTGAGG AACATGCCAT ACAAGATTTG 
CTGGTTTTCT ATGAGGCGCT TGGCAACCCG GATGAGTTTG TCATCGTGGA AAGGACACCA 
CAGGGGCCAC TGGCAGTGCC TATGTGGAAC AAACACGGAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017656171.1
CDS	522..4586
Translation	MNSATPDCTPKCRSLQHAADVLSVVTKGSEKQIKTFLASHCYSAATVKDAFGRNAVHLASSCGKKGVLDWLLERGVDPLVKDKESGWTALHRSIFYGHIDCLWSLLKHGVSLYMQDKEGLSPLDLLMKDRPAHVVFHNTDPTEVYTWGDNTNFTLGHGNQNSKHHPELVDLFSRGGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLVPRLVEGLSGHNCSQVAAAKDHTVVLTEDGCVYTFGLNTFHQLGITPPPSSCNVPRQMQAKYLKGRTVIGVAAGRFHTVLWTRDAVYTMGLNGGQLGYLLDPNGEKCVIAPRQVSALHHKDVALSLVAASDGATVCVTTRGDIYLLADYQCKKIASKQLNLEKVLVSGGCMEYKVDPEHLKENGGQKICVLAMDGAGRVFCWRSVHSSLKQCRWAYPRQVLISDIALNRNEVLFVTQDGEGFKGKWFEGKRKNSEKKDILPNLHHSSSDVSCVSDTNSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDEMDNIHDVTFQVGTRTFPAHKYVLAVRSDFFQKLFLSDGTPLEVSDVYRKEEDSAGCHLFVVEKVHPDLFECLLQFMYTDTCDFLTHGFKPRIFINKKSEEYQGSLNSLMNTMNCPEDETQKSAFEVYKSSQAHTVSEKQKGKPKSSKKGRSASEDDPVKMLQNVAKKFGLSNLSGRLDGVRFENEKINVIAKKTGNKLKLSQKKCSFLCDVIMKSVDGKEYPCHKCVLCARLEYFHSMLSSSWIEASSGAALEMPVRSEVLRVILDYLYTDEALLIKESQNVDFICSVLVVADQLLITRLKEICEVALTEKLTLKNAAMLLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDDVLKDLSIFYRKMIPAMDRRIITPYQDGPDLSCLEVEDGAVFLKEEITVEQNYSETMFKKAKTKAKKKPRKRSDSSGGYNLSDIIQTPPSAGLLKSGKTSSVESLPELLTSDSEGSYTGVGSPRDLQSPDFTAGFNSDKIEGKAKSYVNGTPPMCSREGFKPWEKSPASTSAPQLIPSNRADTPGSSIWVASSCSPVSPPVVDLRTIMEIEESRKKCGTTPKLNLGKIVSHGVKLSQKQRKMIALTTKDNLSGTGNMETVVVAPSKPPKPVNARASSLQSPSSKSFWDFLLEEKKSIIGHSSGDQDHVKKVCFKGNENSQALNVVRCSTHGSPGLENNLISDLPLLDSPNPWLSSSLTASTTAAPVTFASIVEEELQQEAALIRSREKPLALIQVEEHAIQDLLVFYEALGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_017800682.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017800682.2

ORF Insert Sequence:

ATGAACTCTG CCACCCCCGA CTGCACCCCC AAATGCCGAT CCCTGCAACA CGCTGCAGAC 
GTCCTTTCCG TGGTAACCAA GGGGAGTGAA AAGCAGATTA AGACCTTCCT CGCCAGCCAC 
TGCTACAGTG CTGCGACTGT CAAAGATGCC TTTGGCAGGA ATGCTGTCCA CCTGGCCTCC 
TCCTGTGGGA AGAAAGGCGT GCTAGACTGG CTTTTGGAGA GGGGAGTGGA TCCACTTGTG 
AAAGACAAGG AGTCAGGCTG GACTGCGCTG CACAGGAGCA TTTTCTATGG ACATATTGAT 
TGTCTTTGGT CTCTGTTGAA GCATGGTGTT AGTCTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTAAT GAAGGATAGA CCAGCTCATG TAGTATTTCA CAACACTGAC 
CCCACAGAAG TCTACACTTG GGGAGATAAT ACAAACTTTA CTCTGGGTCA TGGAAACCAG 
AACAGCAAAC ATCACCCAGA ACTGGTGGAT TTGTTCTCCA GGGGTGGCGT TTATATCAAA 
CAGGTGGTGC TTTGCAAATT TCATTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC 
TGTGGGCATG GTCCAGGAGG AAGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGACTTGTGG AAGGACTGAG TGGTCATAAC TGTTCCCAGG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACCTTTG GTCTCAATAC TTTTCACCAA 
TTAGGAATTA CTCCTCCTCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC 
CTGAAAGGAA GAACTGTCAT TGGAGTAGCA GCAGGCAGGT TTCACACAGT TCTGTGGACT 
CGAGATGCCG TTTACACTAT GGGCCTGAAT GGTGGACAGC TGGGTTACTT ACTGGATCCC 
AATGGAGAAA AGTGTGTCAT AGCTCCTCGC CAGGTCTCTG CCCTTCATCA TAAGGATGTT 
GCTCTGTCTT TGGTTGCTGC AAGTGATGGT GCCACAGTCT GTGTTACCAC AAGAGGAGAT 
ATATACTTAC TTGCAGACTA TCAGTGCAAG AAGATAGCAT CTAAACAACT GAACTTGGAA 
AAGGTTCTTG TATCTGGGGG TTGTATGGAA TACAAAGTCG ATCCTGAACA TTTGAAAGAA 
AATGGGGGTC AGAAAATCTG CGTTCTTGCT ATGGATGGAG CCGGAAGAGT ATTTTGCTGG 
AGATCAGTCC ACAGTTCTCT GAAGCAATGC CGATGGGCCT ATCCTCGCCA GGTGTTAATT 
TCTGATATTG CTTTAAATAG AAATGAAGTT CTGTTTGTCA CCCAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGG AAAAAGAAAG AATTCTGAAA AGAAAGACAT TTTACCAAAC 
CTACATCATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA CAAATAGTGT GTATGAAAGA 
ATTCGACTAG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGACCCAAGT 
GGATGCAACT TTGCAATCCT ACAGTCAGAC CCTAAAACAA GCCTTTATGA AATTCCAGCT 
GTGTCTTCAT CATCCTTTTT TGAAGAATTT GGCAAACTCT TGAGGGAGAC AGATGAAATG 
GACAACATTC ACGATGTGAC ATTTCAAGTT GGCACTAGAA CCTTCCCTGC TCATAAATAT 
GTTTTGGCTG TGCGTTCTGA CTTTTTCCAG AAATTGTTTC TTTCAGATGG TACTCCCTTG 
GAAGTCTCAG ATGTGTACCG GAAAGAGGAA GATTCTGCAG GGTGCCATCT CTTTGTGGTG 
GAGAAGGTTC ATCCTGATTT GTTTGAATGC CTTTTACAAT TCATGTATAC AGATACTTGT 
GACTTTTTAA CTCATGGTTT TAAGCCAAGG ATATTCATAA ACAAAAAGTC AGAAGAATAT 
CAGGGCTCCC TGAATTCTCT TATGAATACG ATGAATTGCC CTGAGGATGA GACCCAGAAG 
TCGGCGTTTG AGGTCTACAA AAGTAGCCAA GCTCACACAG TTAGTGAAAA GCAGAAAGGC 
AAACCCAAAT CTTCTAAAAA GGGAAGAAGT GCTAGTGAAG ATGATCCTGT CAAGATGTTG 
CAAAATGTTG CAAAGAAATT TGGCCTCAGT AATCTGAGTG GCAGGTTAGA TGGAGTCAGA 
TTTGAAAATG AAAAAATTAA TGTTATTGCA AAGAAAACTG GCAATAAATT GAAGCTAAGT 
CAAAAAAAAT GTTCATTTCT GTGTGATGTT ATCATGAAAT CAGTGGATGG AAAGGAATAT 
CCTTGTCATA AATGTGTCCT TTGTGCTAGA CTTGAGTATT TTCATAGTAT GCTGAGTAGC 
TCGTGGATTG AGGCTTCGAG TGGTGCCGCT CTGGAAATGC CAGTACGTTC TGAGGTCCTG 
AGAGTGATTT TGGACTACCT CTACACTGAT GAAGCTCTGC TGATAAAAGA ATCTCAAAAT 
GTGGATTTTA TTTGTAGTGT TCTTGTAGTG GCTGATCAGC TTCTGATAAC TCGATTAAAG 
GAGATTTGTG AAGTGGCATT AACTGAAAAA CTTACCCTTA AGAATGCTGC TATGCTACTG 
GAATTTGCAG CAATGTACAA TGCTGAACAG TTGAAACTGT CTTGTTTACA GTTTATAGGA 
CTGAATATGG CAGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGATGTTTTG 
AAGGATCTTT CCATATTTTA CCGAAAAATG ATCCCAGCAA TGGATAGAAG AATCATTACA 
CCATACCAAG ATGGACCAGA TCTTAGCTGT TTGGAAGTAG AGGATGGAGC TGTCTTTTTG 
AAAGAAGAAA TAACTGTGGA GCAAAATTAT TCGGAAACTA TGTTTAAGAA GGCAAAAACA 
AAAGCTAAAA AGAAGCCACG GAAGCGCTCA GATAGTTCTG GAGGTTACAA CCTTTCAGAT 
ATTATTCAGA CTCCACCATC TGCAGGATTA TTGAAGTCTG GGAAGACCAG TTCTGTGGAA 
TCTCTTCCAG AACTGTTGAC CTCAGATTCT GAAGGAAGCT ACACAGGAGT GGGTAGTCCC 
AGAGACCTGC AGTCCCCTGA TTTCACAGCA GGATTTAATT CAGATAAGAT TGAGGGGAAA 
GCTAAATCGT ATGTGAATGG TACGCCTCCC ATGTGTTCTA GGGAAGGGTT CAAACCATGG 
GAAAAATCAC CAGCATCTAC ATCTGCTCCC CAGCTCATCC CCAGTAACAG AGCTGACACT 
CCAGGCTCAT CCATCTGGGT TGCGAGCTCT TGCAGTCCTG TGAGCCCTCC TGTTGTGGAT 
CTCAGAACCA TCATGGAAAT AGAAGAAAGT AGAAAAAAAT GTGGCACAAC ACCAAAATTG 
AACTTAGGAA AAATTGTTTC TCATGGAGTT AAACTTTCTC AGAAACAACG AAAAATGATT 
GCATTGACAA CAAAAGACAA CCTTTCAGGA ACAGGCAACA TGGAAACAGT TGTTGTAGCT 
CCTTCCAAGC CTCCTAAGCC AGTGAATGCA CGGGCATCAT CTCTACAGTC CCCTTCATCC 
AAGTCATTCT GGGATTTCTT ACTAGAAGAA AAAAAGTCTA TTATTGGCCA TAGTTCAGGT 
GATCAAGATC ATGTCAAAAA AGTTTGTTTT AAAGGAAATG AAAATTCTCA GGCTCTAAAT 
GTTGTAAGGT GCTCAACTCA TGGAAGCCCA GGACTAGAAA ACAACCTTAT TTCTGATTTA 
CCACTTCTAG ACAGTCCCAA TCCCTGGCTG TCGTCTTCGT TGACTGCTTC TACCACAGCA 
GCTCCGGTCA CCTTCGCATC AATTGTAGAA GAGGAGCTGC AGCAGGAAGC AGCTCTTATC 
AGAAGTCGGG AAAAACCCTT GGCCCTGATT CAGGTTGAGG AACATGCCAT ACAAGATTTG 
CTGGTTTTCT ATGAGGCGCT TGGCAACCCG GATGAGTTTG TCATCGTGGA AAGGACACCA 
CAGGGGCCAC TGGCAGTGCC TATGTGGAAC AAACACGGAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ibtk ( XM_017800683.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017656172.1 Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X3, mRNA.

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CloneID	ONa25778
Clone ID Related Accession (Same CDS sequence)	XM_017800683.1 , XM_017800683.2
Accession Version	XM_017800683.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	inhibitor of Bruton tyrosine kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008346901.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTG CCACCCCCGA CTGCACCCCC AAATGCCGAT CCCTGCAACA CGCTGCAGAC 
GTCCTTTCCG TGGTAACCAA GGGGAGTGAA AAGCAGATTA AGACCTTCCT CGCCAGCCAC 
TGCTACAGTG CTGCGACTGT CAAAGATGCC TTTGGCAGGA ATGCTGTCCA CCTGGCCTCC 
TCCTGTGGGA AGAAAGGCGT GCTAGACTGG CTTTTGGAGA GGGGAGTGGA TCCACTTGTG 
AAAGACAAGG AGTCAGGCTG GACTGCGCTG CACAGGAGCA TTTTCTATGG ACATATTGAT 
TGTCTTTGGT CTCTGTTGAA GCATGGTGTT AGTCTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTAAT GAAGGATAGA CCAGCTCATG TAGTATTTCA CAACACTGAC 
CCCACAGAAG TCTACACTTG GGGAGATAAT ACAAACTTTA CTCTGGGTCA TGGAAACCAG 
AACAGCAAAC ATCACCCAGA ACTGGTGGAT TTGTTCTCCA GGGGTGGCGT TTATATCAAA 
CAGGTGGTGC TTTGCAAATT TCATTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC 
TGTGGGCATG GTCCAGGAGG AAGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGACTTGTGG AAGGACTGAG TGGTCATAAC TGTTCCCAGG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACCTTTG GTCTCAATAC TTTTCACCAA 
TTAGGAATTA CTCCTCCTCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC 
CTGAAAGGAA GAACTGTCAT TGGAGTAGCA GCAGGCAGGT TTCACACAGT TCTGTGGACT 
CGAGATGCCG TTTACACTAT GGGCCTGAAT GGTGGACAGC TGGGTTACTT ACTGGATCCC 
AATGGAGAAA AGTGTGTCAT AGCTCCTCGC CAGGTCTCTG CCCTTCATCA TAAGGATGTT 
GCTCTGTCTT TGGTTGCTGC AAGTGATGGT GCCACAGTCT GTGTTACCAC AAGAGGAGAT 
ATATACTTAC TTGCAGACTA TCAGTGCAAG AAGATAGCAT CTAAACAACT GAACTTGGAA 
AAGGTTCTTG TATCTGGGGG TTGTATGGAA TACAAAGTCG ATCCTGAACA TTTGAAAGAA 
AATGGGGGTC AGAAAATCTG CGTTCTTGCT ATGGATGGAG CCGGAAGAGT ATTTTGCTGG 
AGATCAGTCC ACAGTTCTCT GAAGCAATGC CGATGGGCCT ATCCTCGCCA GGTGTTAATT 
TCTGATATTG CTTTAAATAG AAATGAAGTT CTGTTTGTCA CCCAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGG AAAAAGAAAG AATTCTGAAA AGAAAGCAGA CATTTTACCA 
AACCTACATC ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATACAAATAG TGTGTATGAA 
AGAATTCGAC TAGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGACCCA 
AGTGGATGCA ACTTTGCAAT CCTACAGTCA GACCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCTGTGTCTT CATCATCCTT TTTTGAAGAA TTTGGCAAAC TCTTGAGGGA GACAGATGAA 
ATGGACAACA TTCACGATGT GACATTTCAA GTTGGCACTA GAACCTTCCC TGCTCATAAA 
TATGTTTTGG CTGTGCGTTC TGACTTTTTC CAGAAATTGT TTCTTTCAGA TGGTACTCCC 
TTGGAAGTCT CAGATGTGTA CCGGAAAGAG GAAGATTCTG CAGGGTGCCA TCTCTTTGTG 
GTGGAGAAGG TTCATCCTGA TTTGTTTGAA TGCCTTTTAC AATTCATGTA TACAGATACT 
TGTGACTTTT TAACTCATGG TTTTAAGCCA AGGATATTCA TAAACAAAAA GTCAGAAGAA 
TATCAGGGCT CCCTGAATTC TCTTATGAAT ACGATGAATT GCCCTGAGGA TGAGACCCAG 
AAGTCGGCGT TTGAGGTCTA CAAAAGTAGC CAAGCTCACA CAGTTAGTGA AAAGCAGAAA 
GGCAAACCCA AATCTTCTAA AAAGGGAAGA AGTGCTAGTG AAGATGATCC TGTCAAGATG 
TTGCAAAATG TTGCAAAGAA ATTTGGCCTC AGTAATCTGA GTGGCAGGTT AGATGGAGTC 
AGATTTGAAA ATGAAAAAAT TAATGTTATT GCAAAGAAAA CTGGCAATAA ATTGAAGCTA 
AGTCAAAAAA AATGTTCATT TCTGTGTGAT GTTATCATGA AATCAGTGGA TGGAAAGGAA 
TATCCTTGTC ATAAATGTGT CCTTTGTGCT AGACTTGAGT ATTTTCATAG TATGCTGAGT 
AGCTCGTGGA TTGAGGCTTC GAGTGGTGCC GCTCTGGAAA TGCCAGTACG TTCTGAGGTC 
CTGAGAGTGA TTTTGGACTA CCTCTACACT GATGAAGCTC TGCTGATAAA AGAATCTCAA 
AATGTGGATT TTATTTGTAG TGTTCTTGTA GTGGCTGATC AGCTTCTGAT AACTCGATTA 
AAGGAGATTT GTGAAGTGGC ATTAACTGAA AAACTTACCC TTAAGAATGC TGCTATGCTA 
CTGGAATTTG CAGCAATGTA CAATGCTGAA CAGTTGAAAC TGTCTTGTTT ACAGTTTATA 
GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGGTCTCTTG ATGTTTTAAG TGATGATGTT 
TTGAAGGATC TTTCCATATT TTACCGAAAA ATGATCCCAG CAATGGATAG AAGAATCATT 
ACACCATACC AAGATGGACC AGATCTTAGC TGTTTGGAAG TAGAGGATGG AGCTGTCTTT 
TTGAAAGAAG AAATAACTGT GGAGCAAAAT TATTCGGAAA CTATGTTTAA GAAGGCAAAA 
ACAAAAGCTA AAAAGAAGCC ACGGAAGCGC TCAGATAGTT CTGGAGGTTA CAACCTTTCA 
GATATTATTC AGACTCCACC ATCTGCAGGA TTATTGAAGT CTGGGAAGAC CAGTTCTGTG 
GAATCTCTTC CAGAACTGTT GACCTCAGAT TCTGAAGGAA GCTACACAGG AGTGGGTAGT 
CCCAGAGACC TGCAGTCCCC TGATTTCACA GCAGGATTTA ATTCAGATAA GATTGAGGGG 
AAAGCTAAAT CGTATGTGAA TGGTACGCCT CCCATGTGTT CTAGGGAAGG GTTCAAACCA 
TGGGAAAAAT CACCAGCATC TACATCTGCT CCCCAGCTCA TCCCCAGTAA CAGAGCTGAC 
ACTCCAGGCT CATCCATCTG GGTTGCGAGC TCTTGCAGTC CTGTGAGCCC TCCTGTTGTG 
GATCTCAGAA CCATCATGGA AATAGAAGAA AGTAGAAAAA AATGTGGCAC AACACCAAAA 
TTGAACTTAG GAAAAATTGT TTCTCATGGA GTTAAACTTT CTCAGAAACA ACGAAAAATG 
ATTGCATTGA CAACAAAAGA CAACCTTTCA GGAACAGGCA ACATGGAAAC AGTTGTTGTA 
GCTCCTTCCA AGCCTCCTAA GCCAGTGAAT GCACGGTGCT CAACTCATGG AAGCCCAGGA 
CTAGAAAACA ACCTTATTTC TGATTTACCA CTTCTAGACA GTCCCAATCC CTGGCTGTCG 
TCTTCGTTGA CTGCTTCTAC CACAGCAGCT CCGGTCACCT TCGCATCAAT TGTAGAAGAG 
GAGCTGCAGC AGGAAGCAGC TCTTATCAGA AGTCGGGAAA AACCCTTGGC CCTGATTCAG 
GTTGAGGAAC ATGCCATACA AGATTTGCTG GTTTTCTATG AGGCGCTTGG CAACCCGGAT 
GAGTTTGTCA TCGTGGAAAG GACACCACAG GGGCCACTGG CAGTGCCTAT GTGGAACAAA 
CACGGATGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017656172.1
CDS	524..4435
Translation	MNSATPDCTPKCRSLQHAADVLSVVTKGSEKQIKTFLASHCYSAATVKDAFGRNAVHLASSCGKKGVLDWLLERGVDPLVKDKESGWTALHRSIFYGHIDCLWSLLKHGVSLYMQDKEGLSPLDLLMKDRPAHVVFHNTDPTEVYTWGDNTNFTLGHGNQNSKHHPELVDLFSRGGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLVPRLVEGLSGHNCSQVAAAKDHTVVLTEDGCVYTFGLNTFHQLGITPPPSSCNVPRQMQAKYLKGRTVIGVAAGRFHTVLWTRDAVYTMGLNGGQLGYLLDPNGEKCVIAPRQVSALHHKDVALSLVAASDGATVCVTTRGDIYLLADYQCKKIASKQLNLEKVLVSGGCMEYKVDPEHLKENGGQKICVLAMDGAGRVFCWRSVHSSLKQCRWAYPRQVLISDIALNRNEVLFVTQDGEGFKGKWFEGKRKNSEKKADILPNLHHSSSDVSCVSDTNSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDEMDNIHDVTFQVGTRTFPAHKYVLAVRSDFFQKLFLSDGTPLEVSDVYRKEEDSAGCHLFVVEKVHPDLFECLLQFMYTDTCDFLTHGFKPRIFINKKSEEYQGSLNSLMNTMNCPEDETQKSAFEVYKSSQAHTVSEKQKGKPKSSKKGRSASEDDPVKMLQNVAKKFGLSNLSGRLDGVRFENEKINVIAKKTGNKLKLSQKKCSFLCDVIMKSVDGKEYPCHKCVLCARLEYFHSMLSSSWIEASSGAALEMPVRSEVLRVILDYLYTDEALLIKESQNVDFICSVLVVADQLLITRLKEICEVALTEKLTLKNAAMLLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDDVLKDLSIFYRKMIPAMDRRIITPYQDGPDLSCLEVEDGAVFLKEEITVEQNYSETMFKKAKTKAKKKPRKRSDSSGGYNLSDIIQTPPSAGLLKSGKTSSVESLPELLTSDSEGSYTGVGSPRDLQSPDFTAGFNSDKIEGKAKSYVNGTPPMCSREGFKPWEKSPASTSAPQLIPSNRADTPGSSIWVASSCSPVSPPVVDLRTIMEIEESRKKCGTTPKLNLGKIVSHGVKLSQKQRKMIALTTKDNLSGTGNMETVVVAPSKPPKPVNARCSTHGSPGLENNLISDLPLLDSPNPWLSSSLTASTTAAPVTFASIVEEELQQEAALIRSREKPLALIQVEEHAIQDLLVFYEALGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_017800683.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017800683.1

ORF Insert Sequence:

ATGAACTCTG CCACCCCCGA CTGCACCCCC AAATGCCGAT CCCTGCAACA CGCTGCAGAC 
GTCCTTTCCG TGGTAACCAA GGGGAGTGAA AAGCAGATTA AGACCTTCCT CGCCAGCCAC 
TGCTACAGTG CTGCGACTGT CAAAGATGCC TTTGGCAGGA ATGCTGTCCA CCTGGCCTCC 
TCCTGTGGGA AGAAAGGCGT GCTAGACTGG CTTTTGGAGA GGGGAGTGGA TCCACTTGTG 
AAAGACAAGG AGTCAGGCTG GACTGCGCTG CACAGGAGCA TTTTCTATGG ACATATTGAT 
TGTCTTTGGT CTCTGTTGAA GCATGGTGTT AGTCTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTAAT GAAGGATAGA CCAGCTCATG TAGTATTTCA CAACACTGAC 
CCCACAGAAG TCTACACTTG GGGAGATAAT ACAAACTTTA CTCTGGGTCA TGGAAACCAG 
AACAGCAAAC ATCACCCAGA ACTGGTGGAT TTGTTCTCCA GGGGTGGCGT TTATATCAAA 
CAGGTGGTGC TTTGCAAATT TCATTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC 
TGTGGGCATG GTCCAGGAGG AAGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGACTTGTGG AAGGACTGAG TGGTCATAAC TGTTCCCAGG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACCTTTG GTCTCAATAC TTTTCACCAA 
TTAGGAATTA CTCCTCCTCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC 
CTGAAAGGAA GAACTGTCAT TGGAGTAGCA GCAGGCAGGT TTCACACAGT TCTGTGGACT 
CGAGATGCCG TTTACACTAT GGGCCTGAAT GGTGGACAGC TGGGTTACTT ACTGGATCCC 
AATGGAGAAA AGTGTGTCAT AGCTCCTCGC CAGGTCTCTG CCCTTCATCA TAAGGATGTT 
GCTCTGTCTT TGGTTGCTGC AAGTGATGGT GCCACAGTCT GTGTTACCAC AAGAGGAGAT 
ATATACTTAC TTGCAGACTA TCAGTGCAAG AAGATAGCAT CTAAACAACT GAACTTGGAA 
AAGGTTCTTG TATCTGGGGG TTGTATGGAA TACAAAGTCG ATCCTGAACA TTTGAAAGAA 
AATGGGGGTC AGAAAATCTG CGTTCTTGCT ATGGATGGAG CCGGAAGAGT ATTTTGCTGG 
AGATCAGTCC ACAGTTCTCT GAAGCAATGC CGATGGGCCT ATCCTCGCCA GGTGTTAATT 
TCTGATATTG CTTTAAATAG AAATGAAGTT CTGTTTGTCA CCCAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGG AAAAAGAAAG AATTCTGAAA AGAAAGCAGA CATTTTACCA 
AACCTACATC ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATACAAATAG TGTGTATGAA 
AGAATTCGAC TAGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGACCCA 
AGTGGATGCA ACTTTGCAAT CCTACAGTCA GACCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCTGTGTCTT CATCATCCTT TTTTGAAGAA TTTGGCAAAC TCTTGAGGGA GACAGATGAA 
ATGGACAACA TTCACGATGT GACATTTCAA GTTGGCACTA GAACCTTCCC TGCTCATAAA 
TATGTTTTGG CTGTGCGTTC TGACTTTTTC CAGAAATTGT TTCTTTCAGA TGGTACTCCC 
TTGGAAGTCT CAGATGTGTA CCGGAAAGAG GAAGATTCTG CAGGGTGCCA TCTCTTTGTG 
GTGGAGAAGG TTCATCCTGA TTTGTTTGAA TGCCTTTTAC AATTCATGTA TACAGATACT 
TGTGACTTTT TAACTCATGG TTTTAAGCCA AGGATATTCA TAAACAAAAA GTCAGAAGAA 
TATCAGGGCT CCCTGAATTC TCTTATGAAT ACGATGAATT GCCCTGAGGA TGAGACCCAG 
AAGTCGGCGT TTGAGGTCTA CAAAAGTAGC CAAGCTCACA CAGTTAGTGA AAAGCAGAAA 
GGCAAACCCA AATCTTCTAA AAAGGGAAGA AGTGCTAGTG AAGATGATCC TGTCAAGATG 
TTGCAAAATG TTGCAAAGAA ATTTGGCCTC AGTAATCTGA GTGGCAGGTT AGATGGAGTC 
AGATTTGAAA ATGAAAAAAT TAATGTTATT GCAAAGAAAA CTGGCAATAA ATTGAAGCTA 
AGTCAAAAAA AATGTTCATT TCTGTGTGAT GTTATCATGA AATCAGTGGA TGGAAAGGAA 
TATCCTTGTC ATAAATGTGT CCTTTGTGCT AGACTTGAGT ATTTTCATAG TATGCTGAGT 
AGCTCGTGGA TTGAGGCTTC GAGTGGTGCC GCTCTGGAAA TGCCAGTACG TTCTGAGGTC 
CTGAGAGTGA TTTTGGACTA CCTCTACACT GATGAAGCTC TGCTGATAAA AGAATCTCAA 
AATGTGGATT TTATTTGTAG TGTTCTTGTA GTGGCTGATC AGCTTCTGAT AACTCGATTA 
AAGGAGATTT GTGAAGTGGC ATTAACTGAA AAACTTACCC TTAAGAATGC TGCTATGCTA 
CTGGAATTTG CAGCAATGTA CAATGCTGAA CAGTTGAAAC TGTCTTGTTT ACAGTTTATA 
GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGGTCTCTTG ATGTTTTAAG TGATGATGTT 
TTGAAGGATC TTTCCATATT TTACCGAAAA ATGATCCCAG CAATGGATAG AAGAATCATT 
ACACCATACC AAGATGGACC AGATCTTAGC TGTTTGGAAG TAGAGGATGG AGCTGTCTTT 
TTGAAAGAAG AAATAACTGT GGAGCAAAAT TATTCGGAAA CTATGTTTAA GAAGGCAAAA 
ACAAAAGCTA AAAAGAAGCC ACGGAAGCGC TCAGATAGTT CTGGAGGTTA CAACCTTTCA 
GATATTATTC AGACTCCACC ATCTGCAGGA TTATTGAAGT CTGGGAAGAC CAGTTCTGTG 
GAATCTCTTC CAGAACTGTT GACCTCAGAT TCTGAAGGAA GCTACACAGG AGTGGGTAGT 
CCCAGAGACC TGCAGTCCCC TGATTTCACA GCAGGATTTA ATTCAGATAA GATTGAGGGG 
AAAGCTAAAT CGTATGTGAA TGGTACGCCT CCCATGTGTT CTAGGGAAGG GTTCAAACCA 
TGGGAAAAAT CACCAGCATC TACATCTGCT CCCCAGCTCA TCCCCAGTAA CAGAGCTGAC 
ACTCCAGGCT CATCCATCTG GGTTGCGAGC TCTTGCAGTC CTGTGAGCCC TCCTGTTGTG 
GATCTCAGAA CCATCATGGA AATAGAAGAA AGTAGAAAAA AATGTGGCAC AACACCAAAA 
TTGAACTTAG GAAAAATTGT TTCTCATGGA GTTAAACTTT CTCAGAAACA ACGAAAAATG 
ATTGCATTGA CAACAAAAGA CAACCTTTCA GGAACAGGCA ACATGGAAAC AGTTGTTGTA 
GCTCCTTCCA AGCCTCCTAA GCCAGTGAAT GCACGGTGCT CAACTCATGG AAGCCCAGGA 
CTAGAAAACA ACCTTATTTC TGATTTACCA CTTCTAGACA GTCCCAATCC CTGGCTGTCG 
TCTTCGTTGA CTGCTTCTAC CACAGCAGCT CCGGTCACCT TCGCATCAAT TGTAGAAGAG 
GAGCTGCAGC AGGAAGCAGC TCTTATCAGA AGTCGGGAAA AACCCTTGGC CCTGATTCAG 
GTTGAGGAAC ATGCCATACA AGATTTGCTG GTTTTCTATG AGGCGCTTGG CAACCCGGAT 
GAGTTTGTCA TCGTGGAAAG GACACCACAG GGGCCACTGG CAGTGCCTAT GTGGAACAAA 
CACGGATGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ibtk ( XM_017800683.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017656172.1 Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X3, mRNA.

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CloneID	ONa25778
Clone ID Related Accession (Same CDS sequence)	XM_017800683.1 , XM_017800683.2
Accession Version	XM_017800683.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	inhibitor of Bruton tyrosine kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008346901.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_017800683.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTG CCACCCCCGA CTGCACCCCC AAATGCCGAT CCCTGCAACA CGCTGCAGAC 
GTCCTTTCCG TGGTAACCAA GGGGAGTGAA AAGCAGATTA AGACCTTCCT CGCCAGCCAC 
TGCTACAGTG CTGCGACTGT CAAAGATGCC TTTGGCAGGA ATGCTGTCCA CCTGGCCTCC 
TCCTGTGGGA AGAAAGGCGT GCTAGACTGG CTTTTGGAGA GGGGAGTGGA TCCACTTGTG 
AAAGACAAGG AGTCAGGCTG GACTGCGCTG CACAGGAGCA TTTTCTATGG ACATATTGAT 
TGTCTTTGGT CTCTGTTGAA GCATGGTGTT AGTCTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTAAT GAAGGATAGA CCAGCTCATG TAGTATTTCA CAACACTGAC 
CCCACAGAAG TCTACACTTG GGGAGATAAT ACAAACTTTA CTCTGGGTCA TGGAAACCAG 
AACAGCAAAC ATCACCCAGA ACTGGTGGAT TTGTTCTCCA GGGGTGGCGT TTATATCAAA 
CAGGTGGTGC TTTGCAAATT TCATTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC 
TGTGGGCATG GTCCAGGAGG AAGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGACTTGTGG AAGGACTGAG TGGTCATAAC TGTTCCCAGG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACCTTTG GTCTCAATAC TTTTCACCAA 
TTAGGAATTA CTCCTCCTCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC 
CTGAAAGGAA GAACTGTCAT TGGAGTAGCA GCAGGCAGGT TTCACACAGT TCTGTGGACT 
CGAGATGCCG TTTACACTAT GGGCCTGAAT GGTGGACAGC TGGGTTACTT ACTGGATCCC 
AATGGAGAAA AGTGTGTCAT AGCTCCTCGC CAGGTCTCTG CCCTTCATCA TAAGGATGTT 
GCTCTGTCTT TGGTTGCTGC AAGTGATGGT GCCACAGTCT GTGTTACCAC AAGAGGAGAT 
ATATACTTAC TTGCAGACTA TCAGTGCAAG AAGATAGCAT CTAAACAACT GAACTTGGAA 
AAGGTTCTTG TATCTGGGGG TTGTATGGAA TACAAAGTCG ATCCTGAACA TTTGAAAGAA 
AATGGGGGTC AGAAAATCTG CGTTCTTGCT ATGGATGGAG CCGGAAGAGT ATTTTGCTGG 
AGATCAGTCC ACAGTTCTCT GAAGCAATGC CGATGGGCCT ATCCTCGCCA GGTGTTAATT 
TCTGATATTG CTTTAAATAG AAATGAAGTT CTGTTTGTCA CCCAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGG AAAAAGAAAG AATTCTGAAA AGAAAGCAGA CATTTTACCA 
AACCTACATC ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATACAAATAG TGTGTATGAA 
AGAATTCGAC TAGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGACCCA 
AGTGGATGCA ACTTTGCAAT CCTACAGTCA GACCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCTGTGTCTT CATCATCCTT TTTTGAAGAA TTTGGCAAAC TCTTGAGGGA GACAGATGAA 
ATGGACAACA TTCACGATGT GACATTTCAA GTTGGCACTA GAACCTTCCC TGCTCATAAA 
TATGTTTTGG CTGTGCGTTC TGACTTTTTC CAGAAATTGT TTCTTTCAGA TGGTACTCCC 
TTGGAAGTCT CAGATGTGTA CCGGAAAGAG GAAGATTCTG CAGGGTGCCA TCTCTTTGTG 
GTGGAGAAGG TTCATCCTGA TTTGTTTGAA TGCCTTTTAC AATTCATGTA TACAGATACT 
TGTGACTTTT TAACTCATGG TTTTAAGCCA AGGATATTCA TAAACAAAAA GTCAGAAGAA 
TATCAGGGCT CCCTGAATTC TCTTATGAAT ACGATGAATT GCCCTGAGGA TGAGACCCAG 
AAGTCGGCGT TTGAGGTCTA CAAAAGTAGC CAAGCTCACA CAGTTAGTGA AAAGCAGAAA 
GGCAAACCCA AATCTTCTAA AAAGGGAAGA AGTGCTAGTG AAGATGATCC TGTCAAGATG 
TTGCAAAATG TTGCAAAGAA ATTTGGCCTC AGTAATCTGA GTGGCAGGTT AGATGGAGTC 
AGATTTGAAA ATGAAAAAAT TAATGTTATT GCAAAGAAAA CTGGCAATAA ATTGAAGCTA 
AGTCAAAAAA AATGTTCATT TCTGTGTGAT GTTATCATGA AATCAGTGGA TGGAAAGGAA 
TATCCTTGTC ATAAATGTGT CCTTTGTGCT AGACTTGAGT ATTTTCATAG TATGCTGAGT 
AGCTCGTGGA TTGAGGCTTC GAGTGGTGCC GCTCTGGAAA TGCCAGTACG TTCTGAGGTC 
CTGAGAGTGA TTTTGGACTA CCTCTACACT GATGAAGCTC TGCTGATAAA AGAATCTCAA 
AATGTGGATT TTATTTGTAG TGTTCTTGTA GTGGCTGATC AGCTTCTGAT AACTCGATTA 
AAGGAGATTT GTGAAGTGGC ATTAACTGAA AAACTTACCC TTAAGAATGC TGCTATGCTA 
CTGGAATTTG CAGCAATGTA CAATGCTGAA CAGTTGAAAC TGTCTTGTTT ACAGTTTATA 
GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGGTCTCTTG ATGTTTTAAG TGATGATGTT 
TTGAAGGATC TTTCCATATT TTACCGAAAA ATGATCCCAG CAATGGATAG AAGAATCATT 
ACACCATACC AAGATGGACC AGATCTTAGC TGTTTGGAAG TAGAGGATGG AGCTGTCTTT 
TTGAAAGAAG AAATAACTGT GGAGCAAAAT TATTCGGAAA CTATGTTTAA GAAGGCAAAA 
ACAAAAGCTA AAAAGAAGCC ACGGAAGCGC TCAGATAGTT CTGGAGGTTA CAACCTTTCA 
GATATTATTC AGACTCCACC ATCTGCAGGA TTATTGAAGT CTGGGAAGAC CAGTTCTGTG 
GAATCTCTTC CAGAACTGTT GACCTCAGAT TCTGAAGGAA GCTACACAGG AGTGGGTAGT 
CCCAGAGACC TGCAGTCCCC TGATTTCACA GCAGGATTTA ATTCAGATAA GATTGAGGGG 
AAAGCTAAAT CGTATGTGAA TGGTACGCCT CCCATGTGTT CTAGGGAAGG GTTCAAACCA 
TGGGAAAAAT CACCAGCATC TACATCTGCT CCCCAGCTCA TCCCCAGTAA CAGAGCTGAC 
ACTCCAGGCT CATCCATCTG GGTTGCGAGC TCTTGCAGTC CTGTGAGCCC TCCTGTTGTG 
GATCTCAGAA CCATCATGGA AATAGAAGAA AGTAGAAAAA AATGTGGCAC AACACCAAAA 
TTGAACTTAG GAAAAATTGT TTCTCATGGA GTTAAACTTT CTCAGAAACA ACGAAAAATG 
ATTGCATTGA CAACAAAAGA CAACCTTTCA GGAACAGGCA ACATGGAAAC AGTTGTTGTA 
GCTCCTTCCA AGCCTCCTAA GCCAGTGAAT GCACGGTGCT CAACTCATGG AAGCCCAGGA 
CTAGAAAACA ACCTTATTTC TGATTTACCA CTTCTAGACA GTCCCAATCC CTGGCTGTCG 
TCTTCGTTGA CTGCTTCTAC CACAGCAGCT CCGGTCACCT TCGCATCAAT TGTAGAAGAG 
GAGCTGCAGC AGGAAGCAGC TCTTATCAGA AGTCGGGAAA AACCCTTGGC CCTGATTCAG 
GTTGAGGAAC ATGCCATACA AGATTTGCTG GTTTTCTATG AGGCGCTTGG CAACCCGGAT 
GAGTTTGTCA TCGTGGAAAG GACACCACAG GGGCCACTGG CAGTGCCTAT GTGGAACAAA 
CACGGATGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017656172.1
CDS	523..4434
Translation	MNSATPDCTPKCRSLQHAADVLSVVTKGSEKQIKTFLASHCYSAATVKDAFGRNAVHLASSCGKKGVLDWLLERGVDPLVKDKESGWTALHRSIFYGHIDCLWSLLKHGVSLYMQDKEGLSPLDLLMKDRPAHVVFHNTDPTEVYTWGDNTNFTLGHGNQNSKHHPELVDLFSRGGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLVPRLVEGLSGHNCSQVAAAKDHTVVLTEDGCVYTFGLNTFHQLGITPPPSSCNVPRQMQAKYLKGRTVIGVAAGRFHTVLWTRDAVYTMGLNGGQLGYLLDPNGEKCVIAPRQVSALHHKDVALSLVAASDGATVCVTTRGDIYLLADYQCKKIASKQLNLEKVLVSGGCMEYKVDPEHLKENGGQKICVLAMDGAGRVFCWRSVHSSLKQCRWAYPRQVLISDIALNRNEVLFVTQDGEGFKGKWFEGKRKNSEKKADILPNLHHSSSDVSCVSDTNSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDEMDNIHDVTFQVGTRTFPAHKYVLAVRSDFFQKLFLSDGTPLEVSDVYRKEEDSAGCHLFVVEKVHPDLFECLLQFMYTDTCDFLTHGFKPRIFINKKSEEYQGSLNSLMNTMNCPEDETQKSAFEVYKSSQAHTVSEKQKGKPKSSKKGRSASEDDPVKMLQNVAKKFGLSNLSGRLDGVRFENEKINVIAKKTGNKLKLSQKKCSFLCDVIMKSVDGKEYPCHKCVLCARLEYFHSMLSSSWIEASSGAALEMPVRSEVLRVILDYLYTDEALLIKESQNVDFICSVLVVADQLLITRLKEICEVALTEKLTLKNAAMLLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDDVLKDLSIFYRKMIPAMDRRIITPYQDGPDLSCLEVEDGAVFLKEEITVEQNYSETMFKKAKTKAKKKPRKRSDSSGGYNLSDIIQTPPSAGLLKSGKTSSVESLPELLTSDSEGSYTGVGSPRDLQSPDFTAGFNSDKIEGKAKSYVNGTPPMCSREGFKPWEKSPASTSAPQLIPSNRADTPGSSIWVASSCSPVSPPVVDLRTIMEIEESRKKCGTTPKLNLGKIVSHGVKLSQKQRKMIALTTKDNLSGTGNMETVVVAPSKPPKPVNARCSTHGSPGLENNLISDLPLLDSPNPWLSSSLTASTTAAPVTFASIVEEELQQEAALIRSREKPLALIQVEEHAIQDLLVFYEALGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_017800683.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili inhibitor of Bruton tyrosine kinase (Ibtk), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017800683.2

ORF Insert Sequence:

ATGAACTCTG CCACCCCCGA CTGCACCCCC AAATGCCGAT CCCTGCAACA CGCTGCAGAC 
GTCCTTTCCG TGGTAACCAA GGGGAGTGAA AAGCAGATTA AGACCTTCCT CGCCAGCCAC 
TGCTACAGTG CTGCGACTGT CAAAGATGCC TTTGGCAGGA ATGCTGTCCA CCTGGCCTCC 
TCCTGTGGGA AGAAAGGCGT GCTAGACTGG CTTTTGGAGA GGGGAGTGGA TCCACTTGTG 
AAAGACAAGG AGTCAGGCTG GACTGCGCTG CACAGGAGCA TTTTCTATGG ACATATTGAT 
TGTCTTTGGT CTCTGTTGAA GCATGGTGTT AGTCTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTAAT GAAGGATAGA CCAGCTCATG TAGTATTTCA CAACACTGAC 
CCCACAGAAG TCTACACTTG GGGAGATAAT ACAAACTTTA CTCTGGGTCA TGGAAACCAG 
AACAGCAAAC ATCACCCAGA ACTGGTGGAT TTGTTCTCCA GGGGTGGCGT TTATATCAAA 
CAGGTGGTGC TTTGCAAATT TCATTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC 
TGTGGGCATG GTCCAGGAGG AAGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGACTTGTGG AAGGACTGAG TGGTCATAAC TGTTCCCAGG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACCTTTG GTCTCAATAC TTTTCACCAA 
TTAGGAATTA CTCCTCCTCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC 
CTGAAAGGAA GAACTGTCAT TGGAGTAGCA GCAGGCAGGT TTCACACAGT TCTGTGGACT 
CGAGATGCCG TTTACACTAT GGGCCTGAAT GGTGGACAGC TGGGTTACTT ACTGGATCCC 
AATGGAGAAA AGTGTGTCAT AGCTCCTCGC CAGGTCTCTG CCCTTCATCA TAAGGATGTT 
GCTCTGTCTT TGGTTGCTGC AAGTGATGGT GCCACAGTCT GTGTTACCAC AAGAGGAGAT 
ATATACTTAC TTGCAGACTA TCAGTGCAAG AAGATAGCAT CTAAACAACT GAACTTGGAA 
AAGGTTCTTG TATCTGGGGG TTGTATGGAA TACAAAGTCG ATCCTGAACA TTTGAAAGAA 
AATGGGGGTC AGAAAATCTG CGTTCTTGCT ATGGATGGAG CCGGAAGAGT ATTTTGCTGG 
AGATCAGTCC ACAGTTCTCT GAAGCAATGC CGATGGGCCT ATCCTCGCCA GGTGTTAATT 
TCTGATATTG CTTTAAATAG AAATGAAGTT CTGTTTGTCA CCCAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGG AAAAAGAAAG AATTCTGAAA AGAAAGCAGA CATTTTACCA 
AACCTACATC ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATACAAATAG TGTGTATGAA 
AGAATTCGAC TAGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGACCCA 
AGTGGATGCA ACTTTGCAAT CCTACAGTCA GACCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCTGTGTCTT CATCATCCTT TTTTGAAGAA TTTGGCAAAC TCTTGAGGGA GACAGATGAA 
ATGGACAACA TTCACGATGT GACATTTCAA GTTGGCACTA GAACCTTCCC TGCTCATAAA 
TATGTTTTGG CTGTGCGTTC TGACTTTTTC CAGAAATTGT TTCTTTCAGA TGGTACTCCC 
TTGGAAGTCT CAGATGTGTA CCGGAAAGAG GAAGATTCTG CAGGGTGCCA TCTCTTTGTG 
GTGGAGAAGG TTCATCCTGA TTTGTTTGAA TGCCTTTTAC AATTCATGTA TACAGATACT 
TGTGACTTTT TAACTCATGG TTTTAAGCCA AGGATATTCA TAAACAAAAA GTCAGAAGAA 
TATCAGGGCT CCCTGAATTC TCTTATGAAT ACGATGAATT GCCCTGAGGA TGAGACCCAG 
AAGTCGGCGT TTGAGGTCTA CAAAAGTAGC CAAGCTCACA CAGTTAGTGA AAAGCAGAAA 
GGCAAACCCA AATCTTCTAA AAAGGGAAGA AGTGCTAGTG AAGATGATCC TGTCAAGATG 
TTGCAAAATG TTGCAAAGAA ATTTGGCCTC AGTAATCTGA GTGGCAGGTT AGATGGAGTC 
AGATTTGAAA ATGAAAAAAT TAATGTTATT GCAAAGAAAA CTGGCAATAA ATTGAAGCTA 
AGTCAAAAAA AATGTTCATT TCTGTGTGAT GTTATCATGA AATCAGTGGA TGGAAAGGAA 
TATCCTTGTC ATAAATGTGT CCTTTGTGCT AGACTTGAGT ATTTTCATAG TATGCTGAGT 
AGCTCGTGGA TTGAGGCTTC GAGTGGTGCC GCTCTGGAAA TGCCAGTACG TTCTGAGGTC 
CTGAGAGTGA TTTTGGACTA CCTCTACACT GATGAAGCTC TGCTGATAAA AGAATCTCAA 
AATGTGGATT TTATTTGTAG TGTTCTTGTA GTGGCTGATC AGCTTCTGAT AACTCGATTA 
AAGGAGATTT GTGAAGTGGC ATTAACTGAA AAACTTACCC TTAAGAATGC TGCTATGCTA 
CTGGAATTTG CAGCAATGTA CAATGCTGAA CAGTTGAAAC TGTCTTGTTT ACAGTTTATA 
GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGGTCTCTTG ATGTTTTAAG TGATGATGTT 
TTGAAGGATC TTTCCATATT TTACCGAAAA ATGATCCCAG CAATGGATAG AAGAATCATT 
ACACCATACC AAGATGGACC AGATCTTAGC TGTTTGGAAG TAGAGGATGG AGCTGTCTTT 
TTGAAAGAAG AAATAACTGT GGAGCAAAAT TATTCGGAAA CTATGTTTAA GAAGGCAAAA 
ACAAAAGCTA AAAAGAAGCC ACGGAAGCGC TCAGATAGTT CTGGAGGTTA CAACCTTTCA 
GATATTATTC AGACTCCACC ATCTGCAGGA TTATTGAAGT CTGGGAAGAC CAGTTCTGTG 
GAATCTCTTC CAGAACTGTT GACCTCAGAT TCTGAAGGAA GCTACACAGG AGTGGGTAGT 
CCCAGAGACC TGCAGTCCCC TGATTTCACA GCAGGATTTA ATTCAGATAA GATTGAGGGG 
AAAGCTAAAT CGTATGTGAA TGGTACGCCT CCCATGTGTT CTAGGGAAGG GTTCAAACCA 
TGGGAAAAAT CACCAGCATC TACATCTGCT CCCCAGCTCA TCCCCAGTAA CAGAGCTGAC 
ACTCCAGGCT CATCCATCTG GGTTGCGAGC TCTTGCAGTC CTGTGAGCCC TCCTGTTGTG 
GATCTCAGAA CCATCATGGA AATAGAAGAA AGTAGAAAAA AATGTGGCAC AACACCAAAA 
TTGAACTTAG GAAAAATTGT TTCTCATGGA GTTAAACTTT CTCAGAAACA ACGAAAAATG 
ATTGCATTGA CAACAAAAGA CAACCTTTCA GGAACAGGCA ACATGGAAAC AGTTGTTGTA 
GCTCCTTCCA AGCCTCCTAA GCCAGTGAAT GCACGGTGCT CAACTCATGG AAGCCCAGGA 
CTAGAAAACA ACCTTATTTC TGATTTACCA CTTCTAGACA GTCCCAATCC CTGGCTGTCG 
TCTTCGTTGA CTGCTTCTAC CACAGCAGCT CCGGTCACCT TCGCATCAAT TGTAGAAGAG 
GAGCTGCAGC AGGAAGCAGC TCTTATCAGA AGTCGGGAAA AACCCTTGGC CCTGATTCAG 
GTTGAGGAAC ATGCCATACA AGATTTGCTG GTTTTCTATG AGGCGCTTGG CAACCCGGAT 
GAGTTTGTCA TCGTGGAAAG GACACCACAG GGGCCACTGG CAGTGCCTAT GTGGAACAAA 
CACGGATGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Ibtk cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム