Ephb4 cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ephb4
Entrez Gene ID	103733504
Full Name	EPH receptor B4
Gene Type	protein-coding
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008832334.3	XP_008830556.1	ephrin type-B receptor 4
XM_008832334.2	XP_008830556.1	ephrin type-B receptor 4

Pathways from BioSystems
Gene Ontology

The following Ephb4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ephb4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONa15849	XM_008832334.3 Latest version!	Nannospalax galili EPH receptor B4 (Ephb4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
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ONa15849	XM_008832334.2	Nannospalax galili EPH receptor B4 (Ephb4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ephb4 ( XM_008832334.3 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008830556.1 Nannospalax galili EPH receptor B4 (Ephb4), mRNA.

Price & Availability↑

CloneID	ONa15849
Clone ID Related Accession (Same CDS sequence)	XM_008832334.3 , XM_008832334.2
Accession Version	XM_008832334.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	ephrin type-B receptor 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008337205.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008832334.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCC GGGCGCTGCT GTGCTGGGCT TCGGTCGCTA CCGCTTTAGA AGAGACTCTA 
CTGAATACAA AACTGGAAAC CGCAGATCTG AAATGGGTGA CATACCCACA AGCGGATGGT 
CAGTGGGAGG AGCTGAGTGG TTTGGATGAG GAACAACACA GCGTGCGCAC CTATGAGGTG 
TGCGACGTGG CACGCTCTGG AGGCCAGGCA CGCTGGCTGC GCACCGGCTG GGTACCGCGG 
AGTGGAGCCG TGCATGTGTA TGCCACACTG CGCTTCACCA TGATGGAGTG TCTATCCCTG 
CCACGGGCCA GTCGATCCTG CAAAGAGACC TTCACAGTGT TCTACTATGA AAGCGAAGCT 
GATACAGCCA CAGCCCACAC ACCGGCCTGG ATGGAGAACC CCTACATTAA GGTGGATACA 
GTGGCTGCCG AGCACCTAAC CCGGAAGCGC CCTGGAGCAG AAGCAACTGG GAAGGTCAAC 
ATCAAGACGC TGCGCCTGGG TCCGCTCAGC AAAGCTGGTT TTTACCTCGC TTTCCAGGAC 
CAGGGTGCCT GCATGGCCTT GCTCTCTCTG CACCTATTCT ACAAGAAGTG TTCCCAGCTG 
ACTATGAACT TGACCTACTT CCCAGAGACA GTGCCTCGGG AGCTCGTGGT ACCTGTGGCA 
GGGAGCTGTG TGGCTGACGC CGTCCCTGCC CCTGGCCCCA GCCCCAGTCT CTACTGCCGG 
GAAGATGGAC AGTGGGCTGA GCAACCTGTC ACAGGCTGCA GCTGTGCCCC AGGATTCGAG 
GCTGCTGAGG GAAACAAAAT ATGTAGAGCC TGTGGCCAGG GCACCTTCAA GCCTCAGACA 
GGAGAGGGGT CCTGCCAGCC ATGCCCAGCC AACAGCCACT CTAACACCAT TGGAGCTCCT 
GTCTGCCAGT GCCGTATAGG GTATTATCGG ACTCGGTCAG ACCCTAGGAA TGCGCCTTGC 
ACCACCCCAC CATCTGCTCC ACGAAGTGTG GTTCCCCATC TGAATGGCTC CTCCCTGCGC 
CTGGAATGGA GTGCCCCCCT GGAGTCTGGT GGCCGACAGG ACCTCACTTA TGCCATTCGT 
TGCCGAGAGT GCCGTCCTGG GGGCTCCTGC TTGCCCTGTG GGGTGGATCT GACCTTTGAC 
CCTGGTCCTC GGGACCTCAC TGAGCCCTGG GTATCCATTC GAGGGCTGCG TCCTGACGTC 
ACCTATACCT TTGAGGTCTC TGCCTTGAAT GGTGTGTCTA CCTTAGCCTC TGGACCAGTC 
CCATTTGAAC CTGTCAATGT CACCACAGAC CGTGAGGTGC CCCCTGCAGT GTCTGATATC 
CGTGTGACCC GGTCATCTCC CAGCAGCCTG AGCCTGGCAT GGACTGTTCC CAGAGCACCC 
AGTGGGGCTG TACTGGACTA CGAGGTCAAG TATCACGAGA AGGGCGCAGA GGGCCCCAGC 
AGTGTACGCT TCTTAAAGAC ATCAGAAAAC CGGGCTGAGC TCAGAGGGCT GAAGCGGGGA 
GCCAGCTATC TGGTTCAGGT TCGGGCACGC TCCGAAGCTG GCTATGGCCC CTTTGGGCAG 
GAGCATCACA GTCAGACCCA GATGGACGAA AGTGAGAGCT GGCGGGAGCA GCTGGCCCTG 
ATTGCAGGCA CAGCAGTCGT GGGTGTGGTG CTGGTCCTTG TGGTGGTTGT CATCGCTGTC 
CTCTGCCTCC GGAAGCAGAG CAATGGGAGG GAAGCAGAAT ACTCAGATAA ACATGGGCAG 
TATCTCATCG GGCACGGTAC TAAGGTCTAC ATTGACCCCT TCACTTACGA GGACCCTAAC 
GAGGCAGTGA GGGAATTTGC CAAAGAGATT GACGTCTCCT ATGTCAAGAT TGAAGAGGTA 
ATTGGAGCAG GTGAGTTTGG TGAGGTATGC CGGGGACGAC TAAAGGCACC AGGAAAGAAG 
GAGAGCTGTG TAGCCATCAA GACCCTAAAG GGTGGCTACA CAGAGCGGCA GCGGCGTGAG 
TTCCTGAGTG AAGCATCCAT CATGGGTCAG TTCGAGCACC CCAACATCAT CCGCCTTGAG 
GGTGTTGTCA CCAACAGTGT ACCTGTCATG ATTCTCACTG AATTTATGGA GAATGGAGCC 
CTGGACTCCT TCCTACGGCT GAACGATGGG CAGTTCACAG TCATCCAGCT GGTGGGCATG 
TTGCGGGGCA TCGCCTCAGG CATGCGGTAC CTGGCTGAGA TGAGCTATGT CCACCGAGAC 
CTGGCTGCCC GTAACATCCT GGTCAACAGC AACCTAGTCT GCAAGGTCTC TGACTTTGGC 
CTCTCCCGAT TCCTGGAGGA GAACTCTTCT GATCCTACTT ACACAAGCTC CCTGGGAGGA 
AAGATTCCCA TCCGGTGGAC AGCCCCTGAG GCCATTGCCT TCCGGAAGTT CACATCTGCC 
AGTGACGCTT GGAGCTATGG GATCGTCATG TGGGAGGTCA TGTCATTTGG GGAAAGGCCA 
TATTGGGACA TGAGCAACCA GGATGTGATC AACGCCATTG AACAGGACTA CCGGCTGCCC 
CCACCCCCAG ACTGCCCAAC CTCCCTTCAT CAGCTCATGC TGGACTGTTG GCAGAAGGAC 
CGGAATGCCC GGCCTCGCTT TCCCCAGGTG GTCAGTGCCC TTGACAAGAT GATCCGGAAT 
CCTGCTAGCC TCAAGATTGT AGCCAGGGAG AATGGCGGGG CCTCGCATCC ACTCTTGGAC 
CAGCGGCAGC CTCACTACTC TGCTTTTGGC TCTGTGGGAG AGTGGCTCCG AGCCATCAAG 
ATGGGAAGAT ACGAGGAAAG TTTTGCAGCG GCTGGGTTTG GCTCCTTTGA GGTGGTCAGC 
CAGATTTCTG CAGAGGACCT GCTCCGAATT GGAGTCACTC TGGCAGGACA CCAGAAGAAA 
ATCTTGGCCA GTGTCCAGCA TATGAAGTCC CAGGCCAAGC CAGGAGCCCC TGGTGGGACA 
GGAGCACCAG CTCAGCAATA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008830556.1
CDS	494..3457
Translation	MELRALLCWASVATALEETLLNTKLETADLKWVTYPQADGQWEELSGLDEEQHSVRTYEVCDVARSGGQARWLRTGWVPRSGAVHVYATLRFTMMECLSLPRASRSCKETFTVFYYESEADTATAHTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNIKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCSQLTMNLTYFPETVPRELVVPVAGSCVADAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNKICRACGQGTFKPQTGEGSCQPCPANSHSNTIGAPVCQCRIGYYRTRSDPRNAPCTTPPSAPRSVVPHLNGSSLRLEWSAPLESGGRQDLTYAIRCRECRPGGSCLPCGVDLTFDPGPRDLTEPWVSIRGLRPDVTYTFEVSALNGVSTLASGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSLSLAWTVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQMDESESWREQLALIAGTAVVGVVLVLVVVVIAVLCLRKQSNGREAEYSDKHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSVPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFEVVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGAPGGTGAPAQQY

Target ORF information:

RefSeq Version	XM_008832334.3
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili EPH receptor B4 (Ephb4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008832334.3

ORF Insert Sequence:

ATGGAGCTCC GGGCGCTGCT GTGCTGGGCT TCGGTCGCTA CCGCTTTAGA AGAGACTCTA 
CTGAATACAA AACTGGAAAC CGCAGATCTG AAATGGGTGA CATACCCACA AGCGGATGGT 
CAGTGGGAGG AGCTGAGTGG TTTGGATGAG GAACAACACA GCGTGCGCAC CTATGAGGTG 
TGCGACGTGG CACGCTCTGG AGGCCAGGCA CGCTGGCTGC GCACCGGCTG GGTACCGCGG 
AGTGGAGCCG TGCATGTGTA TGCCACACTG CGCTTCACCA TGATGGAGTG TCTATCCCTG 
CCACGGGCCA GTCGATCCTG CAAAGAGACC TTCACAGTGT TCTACTATGA AAGCGAAGCT 
GATACAGCCA CAGCCCACAC ACCGGCCTGG ATGGAGAACC CCTACATTAA GGTGGATACA 
GTGGCTGCCG AGCACCTAAC CCGGAAGCGC CCTGGAGCAG AAGCAACTGG GAAGGTCAAC 
ATCAAGACGC TGCGCCTGGG TCCGCTCAGC AAAGCTGGTT TTTACCTCGC TTTCCAGGAC 
CAGGGTGCCT GCATGGCCTT GCTCTCTCTG CACCTATTCT ACAAGAAGTG TTCCCAGCTG 
ACTATGAACT TGACCTACTT CCCAGAGACA GTGCCTCGGG AGCTCGTGGT ACCTGTGGCA 
GGGAGCTGTG TGGCTGACGC CGTCCCTGCC CCTGGCCCCA GCCCCAGTCT CTACTGCCGG 
GAAGATGGAC AGTGGGCTGA GCAACCTGTC ACAGGCTGCA GCTGTGCCCC AGGATTCGAG 
GCTGCTGAGG GAAACAAAAT ATGTAGAGCC TGTGGCCAGG GCACCTTCAA GCCTCAGACA 
GGAGAGGGGT CCTGCCAGCC ATGCCCAGCC AACAGCCACT CTAACACCAT TGGAGCTCCT 
GTCTGCCAGT GCCGTATAGG GTATTATCGG ACTCGGTCAG ACCCTAGGAA TGCGCCTTGC 
ACCACCCCAC CATCTGCTCC ACGAAGTGTG GTTCCCCATC TGAATGGCTC CTCCCTGCGC 
CTGGAATGGA GTGCCCCCCT GGAGTCTGGT GGCCGACAGG ACCTCACTTA TGCCATTCGT 
TGCCGAGAGT GCCGTCCTGG GGGCTCCTGC TTGCCCTGTG GGGTGGATCT GACCTTTGAC 
CCTGGTCCTC GGGACCTCAC TGAGCCCTGG GTATCCATTC GAGGGCTGCG TCCTGACGTC 
ACCTATACCT TTGAGGTCTC TGCCTTGAAT GGTGTGTCTA CCTTAGCCTC TGGACCAGTC 
CCATTTGAAC CTGTCAATGT CACCACAGAC CGTGAGGTGC CCCCTGCAGT GTCTGATATC 
CGTGTGACCC GGTCATCTCC CAGCAGCCTG AGCCTGGCAT GGACTGTTCC CAGAGCACCC 
AGTGGGGCTG TACTGGACTA CGAGGTCAAG TATCACGAGA AGGGCGCAGA GGGCCCCAGC 
AGTGTACGCT TCTTAAAGAC ATCAGAAAAC CGGGCTGAGC TCAGAGGGCT GAAGCGGGGA 
GCCAGCTATC TGGTTCAGGT TCGGGCACGC TCCGAAGCTG GCTATGGCCC CTTTGGGCAG 
GAGCATCACA GTCAGACCCA GATGGACGAA AGTGAGAGCT GGCGGGAGCA GCTGGCCCTG 
ATTGCAGGCA CAGCAGTCGT GGGTGTGGTG CTGGTCCTTG TGGTGGTTGT CATCGCTGTC 
CTCTGCCTCC GGAAGCAGAG CAATGGGAGG GAAGCAGAAT ACTCAGATAA ACATGGGCAG 
TATCTCATCG GGCACGGTAC TAAGGTCTAC ATTGACCCCT TCACTTACGA GGACCCTAAC 
GAGGCAGTGA GGGAATTTGC CAAAGAGATT GACGTCTCCT ATGTCAAGAT TGAAGAGGTA 
ATTGGAGCAG GTGAGTTTGG TGAGGTATGC CGGGGACGAC TAAAGGCACC AGGAAAGAAG 
GAGAGCTGTG TAGCCATCAA GACCCTAAAG GGTGGCTACA CAGAGCGGCA GCGGCGTGAG 
TTCCTGAGTG AAGCATCCAT CATGGGTCAG TTCGAGCACC CCAACATCAT CCGCCTTGAG 
GGTGTTGTCA CCAACAGTGT ACCTGTCATG ATTCTCACTG AATTTATGGA GAATGGAGCC 
CTGGACTCCT TCCTACGGCT GAACGATGGG CAGTTCACAG TCATCCAGCT GGTGGGCATG 
TTGCGGGGCA TCGCCTCAGG CATGCGGTAC CTGGCTGAGA TGAGCTATGT CCACCGAGAC 
CTGGCTGCCC GTAACATCCT GGTCAACAGC AACCTAGTCT GCAAGGTCTC TGACTTTGGC 
CTCTCCCGAT TCCTGGAGGA GAACTCTTCT GATCCTACTT ACACAAGCTC CCTGGGAGGA 
AAGATTCCCA TCCGGTGGAC AGCCCCTGAG GCCATTGCCT TCCGGAAGTT CACATCTGCC 
AGTGACGCTT GGAGCTATGG GATCGTCATG TGGGAGGTCA TGTCATTTGG GGAAAGGCCA 
TATTGGGACA TGAGCAACCA GGATGTGATC AACGCCATTG AACAGGACTA CCGGCTGCCC 
CCACCCCCAG ACTGCCCAAC CTCCCTTCAT CAGCTCATGC TGGACTGTTG GCAGAAGGAC 
CGGAATGCCC GGCCTCGCTT TCCCCAGGTG GTCAGTGCCC TTGACAAGAT GATCCGGAAT 
CCTGCTAGCC TCAAGATTGT AGCCAGGGAG AATGGCGGGG CCTCGCATCC ACTCTTGGAC 
CAGCGGCAGC CTCACTACTC TGCTTTTGGC TCTGTGGGAG AGTGGCTCCG AGCCATCAAG 
ATGGGAAGAT ACGAGGAAAG TTTTGCAGCG GCTGGGTTTG GCTCCTTTGA GGTGGTCAGC 
CAGATTTCTG CAGAGGACCT GCTCCGAATT GGAGTCACTC TGGCAGGACA CCAGAAGAAA 
ATCTTGGCCA GTGTCCAGCA TATGAAGTCC CAGGCCAAGC CAGGAGCCCC TGGTGGGACA 
GGAGCACCAG CTCAGCAATA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ephb4 ( XM_008832334.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008830556.1 Nannospalax galili EPH receptor B4 (Ephb4), mRNA.

Price & Availability↑

CloneID	ONa15849
Clone ID Related Accession (Same CDS sequence)	XM_008832334.3 , XM_008832334.2
Accession Version	XM_008832334.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	ephrin type-B receptor 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008337205.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008832334.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCC GGGCGCTGCT GTGCTGGGCT TCGGTCGCTA CCGCTTTAGA AGAGACTCTA 
CTGAATACAA AACTGGAAAC CGCAGATCTG AAATGGGTGA CATACCCACA AGCGGATGGT 
CAGTGGGAGG AGCTGAGTGG TTTGGATGAG GAACAACACA GCGTGCGCAC CTATGAGGTG 
TGCGACGTGG CACGCTCTGG AGGCCAGGCA CGCTGGCTGC GCACCGGCTG GGTACCGCGG 
AGTGGAGCCG TGCATGTGTA TGCCACACTG CGCTTCACCA TGATGGAGTG TCTATCCCTG 
CCACGGGCCA GTCGATCCTG CAAAGAGACC TTCACAGTGT TCTACTATGA AAGCGAAGCT 
GATACAGCCA CAGCCCACAC ACCGGCCTGG ATGGAGAACC CCTACATTAA GGTGGATACA 
GTGGCTGCCG AGCACCTAAC CCGGAAGCGC CCTGGAGCAG AAGCAACTGG GAAGGTCAAC 
ATCAAGACGC TGCGCCTGGG TCCGCTCAGC AAAGCTGGTT TTTACCTCGC TTTCCAGGAC 
CAGGGTGCCT GCATGGCCTT GCTCTCTCTG CACCTATTCT ACAAGAAGTG TTCCCAGCTG 
ACTATGAACT TGACCTACTT CCCAGAGACA GTGCCTCGGG AGCTCGTGGT ACCTGTGGCA 
GGGAGCTGTG TGGCTGACGC CGTCCCTGCC CCTGGCCCCA GCCCCAGTCT CTACTGCCGG 
GAAGATGGAC AGTGGGCTGA GCAACCTGTC ACAGGCTGCA GCTGTGCCCC AGGATTCGAG 
GCTGCTGAGG GAAACAAAAT ATGTAGAGCC TGTGGCCAGG GCACCTTCAA GCCTCAGACA 
GGAGAGGGGT CCTGCCAGCC ATGCCCAGCC AACAGCCACT CTAACACCAT TGGAGCTCCT 
GTCTGCCAGT GCCGTATAGG GTATTATCGG ACTCGGTCAG ACCCTAGGAA TGCGCCTTGC 
ACCACCCCAC CATCTGCTCC ACGAAGTGTG GTTCCCCATC TGAATGGCTC CTCCCTGCGC 
CTGGAATGGA GTGCCCCCCT GGAGTCTGGT GGCCGACAGG ACCTCACTTA TGCCATTCGT 
TGCCGAGAGT GCCGTCCTGG GGGCTCCTGC TTGCCCTGTG GGGTGGATCT GACCTTTGAC 
CCTGGTCCTC GGGACCTCAC TGAGCCCTGG GTATCCATTC GAGGGCTGCG TCCTGACGTC 
ACCTATACCT TTGAGGTCTC TGCCTTGAAT GGTGTGTCTA CCTTAGCCTC TGGACCAGTC 
CCATTTGAAC CTGTCAATGT CACCACAGAC CGTGAGGTGC CCCCTGCAGT GTCTGATATC 
CGTGTGACCC GGTCATCTCC CAGCAGCCTG AGCCTGGCAT GGACTGTTCC CAGAGCACCC 
AGTGGGGCTG TACTGGACTA CGAGGTCAAG TATCACGAGA AGGGCGCAGA GGGCCCCAGC 
AGTGTACGCT TCTTAAAGAC ATCAGAAAAC CGGGCTGAGC TCAGAGGGCT GAAGCGGGGA 
GCCAGCTATC TGGTTCAGGT TCGGGCACGC TCCGAAGCTG GCTATGGCCC CTTTGGGCAG 
GAGCATCACA GTCAGACCCA GATGGACGAA AGTGAGAGCT GGCGGGAGCA GCTGGCCCTG 
ATTGCAGGCA CAGCAGTCGT GGGTGTGGTG CTGGTCCTTG TGGTGGTTGT CATCGCTGTC 
CTCTGCCTCC GGAAGCAGAG CAATGGGAGG GAAGCAGAAT ACTCAGATAA ACATGGGCAG 
TATCTCATCG GGCACGGTAC TAAGGTCTAC ATTGACCCCT TCACTTACGA GGACCCTAAC 
GAGGCAGTGA GGGAATTTGC CAAAGAGATT GACGTCTCCT ATGTCAAGAT TGAAGAGGTA 
ATTGGAGCAG GTGAGTTTGG TGAGGTATGC CGGGGACGAC TAAAGGCACC AGGAAAGAAG 
GAGAGCTGTG TAGCCATCAA GACCCTAAAG GGTGGCTACA CAGAGCGGCA GCGGCGTGAG 
TTCCTGAGTG AAGCATCCAT CATGGGTCAG TTCGAGCACC CCAACATCAT CCGCCTTGAG 
GGTGTTGTCA CCAACAGTGT ACCTGTCATG ATTCTCACTG AATTTATGGA GAATGGAGCC 
CTGGACTCCT TCCTACGGCT GAACGATGGG CAGTTCACAG TCATCCAGCT GGTGGGCATG 
TTGCGGGGCA TCGCCTCAGG CATGCGGTAC CTGGCTGAGA TGAGCTATGT CCACCGAGAC 
CTGGCTGCCC GTAACATCCT GGTCAACAGC AACCTAGTCT GCAAGGTCTC TGACTTTGGC 
CTCTCCCGAT TCCTGGAGGA GAACTCTTCT GATCCTACTT ACACAAGCTC CCTGGGAGGA 
AAGATTCCCA TCCGGTGGAC AGCCCCTGAG GCCATTGCCT TCCGGAAGTT CACATCTGCC 
AGTGACGCTT GGAGCTATGG GATCGTCATG TGGGAGGTCA TGTCATTTGG GGAAAGGCCA 
TATTGGGACA TGAGCAACCA GGATGTGATC AACGCCATTG AACAGGACTA CCGGCTGCCC 
CCACCCCCAG ACTGCCCAAC CTCCCTTCAT CAGCTCATGC TGGACTGTTG GCAGAAGGAC 
CGGAATGCCC GGCCTCGCTT TCCCCAGGTG GTCAGTGCCC TTGACAAGAT GATCCGGAAT 
CCTGCTAGCC TCAAGATTGT AGCCAGGGAG AATGGCGGGG CCTCGCATCC ACTCTTGGAC 
CAGCGGCAGC CTCACTACTC TGCTTTTGGC TCTGTGGGAG AGTGGCTCCG AGCCATCAAG 
ATGGGAAGAT ACGAGGAAAG TTTTGCAGCG GCTGGGTTTG GCTCCTTTGA GGTGGTCAGC 
CAGATTTCTG CAGAGGACCT GCTCCGAATT GGAGTCACTC TGGCAGGACA CCAGAAGAAA 
ATCTTGGCCA GTGTCCAGCA TATGAAGTCC CAGGCCAAGC CAGGAGCCCC TGGTGGGACA 
GGAGCACCAG CTCAGCAATA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008830556.1
CDS	494..3457
Translation	MELRALLCWASVATALEETLLNTKLETADLKWVTYPQADGQWEELSGLDEEQHSVRTYEVCDVARSGGQARWLRTGWVPRSGAVHVYATLRFTMMECLSLPRASRSCKETFTVFYYESEADTATAHTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNIKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCSQLTMNLTYFPETVPRELVVPVAGSCVADAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNKICRACGQGTFKPQTGEGSCQPCPANSHSNTIGAPVCQCRIGYYRTRSDPRNAPCTTPPSAPRSVVPHLNGSSLRLEWSAPLESGGRQDLTYAIRCRECRPGGSCLPCGVDLTFDPGPRDLTEPWVSIRGLRPDVTYTFEVSALNGVSTLASGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSLSLAWTVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQMDESESWREQLALIAGTAVVGVVLVLVVVVIAVLCLRKQSNGREAEYSDKHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSVPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFEVVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGAPGGTGAPAQQY

Target ORF information:

RefSeq Version	XM_008832334.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili EPH receptor B4 (Ephb4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008832334.2

ORF Insert Sequence:

ATGGAGCTCC GGGCGCTGCT GTGCTGGGCT TCGGTCGCTA CCGCTTTAGA AGAGACTCTA 
CTGAATACAA AACTGGAAAC CGCAGATCTG AAATGGGTGA CATACCCACA AGCGGATGGT 
CAGTGGGAGG AGCTGAGTGG TTTGGATGAG GAACAACACA GCGTGCGCAC CTATGAGGTG 
TGCGACGTGG CACGCTCTGG AGGCCAGGCA CGCTGGCTGC GCACCGGCTG GGTACCGCGG 
AGTGGAGCCG TGCATGTGTA TGCCACACTG CGCTTCACCA TGATGGAGTG TCTATCCCTG 
CCACGGGCCA GTCGATCCTG CAAAGAGACC TTCACAGTGT TCTACTATGA AAGCGAAGCT 
GATACAGCCA CAGCCCACAC ACCGGCCTGG ATGGAGAACC CCTACATTAA GGTGGATACA 
GTGGCTGCCG AGCACCTAAC CCGGAAGCGC CCTGGAGCAG AAGCAACTGG GAAGGTCAAC 
ATCAAGACGC TGCGCCTGGG TCCGCTCAGC AAAGCTGGTT TTTACCTCGC TTTCCAGGAC 
CAGGGTGCCT GCATGGCCTT GCTCTCTCTG CACCTATTCT ACAAGAAGTG TTCCCAGCTG 
ACTATGAACT TGACCTACTT CCCAGAGACA GTGCCTCGGG AGCTCGTGGT ACCTGTGGCA 
GGGAGCTGTG TGGCTGACGC CGTCCCTGCC CCTGGCCCCA GCCCCAGTCT CTACTGCCGG 
GAAGATGGAC AGTGGGCTGA GCAACCTGTC ACAGGCTGCA GCTGTGCCCC AGGATTCGAG 
GCTGCTGAGG GAAACAAAAT ATGTAGAGCC TGTGGCCAGG GCACCTTCAA GCCTCAGACA 
GGAGAGGGGT CCTGCCAGCC ATGCCCAGCC AACAGCCACT CTAACACCAT TGGAGCTCCT 
GTCTGCCAGT GCCGTATAGG GTATTATCGG ACTCGGTCAG ACCCTAGGAA TGCGCCTTGC 
ACCACCCCAC CATCTGCTCC ACGAAGTGTG GTTCCCCATC TGAATGGCTC CTCCCTGCGC 
CTGGAATGGA GTGCCCCCCT GGAGTCTGGT GGCCGACAGG ACCTCACTTA TGCCATTCGT 
TGCCGAGAGT GCCGTCCTGG GGGCTCCTGC TTGCCCTGTG GGGTGGATCT GACCTTTGAC 
CCTGGTCCTC GGGACCTCAC TGAGCCCTGG GTATCCATTC GAGGGCTGCG TCCTGACGTC 
ACCTATACCT TTGAGGTCTC TGCCTTGAAT GGTGTGTCTA CCTTAGCCTC TGGACCAGTC 
CCATTTGAAC CTGTCAATGT CACCACAGAC CGTGAGGTGC CCCCTGCAGT GTCTGATATC 
CGTGTGACCC GGTCATCTCC CAGCAGCCTG AGCCTGGCAT GGACTGTTCC CAGAGCACCC 
AGTGGGGCTG TACTGGACTA CGAGGTCAAG TATCACGAGA AGGGCGCAGA GGGCCCCAGC 
AGTGTACGCT TCTTAAAGAC ATCAGAAAAC CGGGCTGAGC TCAGAGGGCT GAAGCGGGGA 
GCCAGCTATC TGGTTCAGGT TCGGGCACGC TCCGAAGCTG GCTATGGCCC CTTTGGGCAG 
GAGCATCACA GTCAGACCCA GATGGACGAA AGTGAGAGCT GGCGGGAGCA GCTGGCCCTG 
ATTGCAGGCA CAGCAGTCGT GGGTGTGGTG CTGGTCCTTG TGGTGGTTGT CATCGCTGTC 
CTCTGCCTCC GGAAGCAGAG CAATGGGAGG GAAGCAGAAT ACTCAGATAA ACATGGGCAG 
TATCTCATCG GGCACGGTAC TAAGGTCTAC ATTGACCCCT TCACTTACGA GGACCCTAAC 
GAGGCAGTGA GGGAATTTGC CAAAGAGATT GACGTCTCCT ATGTCAAGAT TGAAGAGGTA 
ATTGGAGCAG GTGAGTTTGG TGAGGTATGC CGGGGACGAC TAAAGGCACC AGGAAAGAAG 
GAGAGCTGTG TAGCCATCAA GACCCTAAAG GGTGGCTACA CAGAGCGGCA GCGGCGTGAG 
TTCCTGAGTG AAGCATCCAT CATGGGTCAG TTCGAGCACC CCAACATCAT CCGCCTTGAG 
GGTGTTGTCA CCAACAGTGT ACCTGTCATG ATTCTCACTG AATTTATGGA GAATGGAGCC 
CTGGACTCCT TCCTACGGCT GAACGATGGG CAGTTCACAG TCATCCAGCT GGTGGGCATG 
TTGCGGGGCA TCGCCTCAGG CATGCGGTAC CTGGCTGAGA TGAGCTATGT CCACCGAGAC 
CTGGCTGCCC GTAACATCCT GGTCAACAGC AACCTAGTCT GCAAGGTCTC TGACTTTGGC 
CTCTCCCGAT TCCTGGAGGA GAACTCTTCT GATCCTACTT ACACAAGCTC CCTGGGAGGA 
AAGATTCCCA TCCGGTGGAC AGCCCCTGAG GCCATTGCCT TCCGGAAGTT CACATCTGCC 
AGTGACGCTT GGAGCTATGG GATCGTCATG TGGGAGGTCA TGTCATTTGG GGAAAGGCCA 
TATTGGGACA TGAGCAACCA GGATGTGATC AACGCCATTG AACAGGACTA CCGGCTGCCC 
CCACCCCCAG ACTGCCCAAC CTCCCTTCAT CAGCTCATGC TGGACTGTTG GCAGAAGGAC 
CGGAATGCCC GGCCTCGCTT TCCCCAGGTG GTCAGTGCCC TTGACAAGAT GATCCGGAAT 
CCTGCTAGCC TCAAGATTGT AGCCAGGGAG AATGGCGGGG CCTCGCATCC ACTCTTGGAC 
CAGCGGCAGC CTCACTACTC TGCTTTTGGC TCTGTGGGAG AGTGGCTCCG AGCCATCAAG 
ATGGGAAGAT ACGAGGAAAG TTTTGCAGCG GCTGGGTTTG GCTCCTTTGA GGTGGTCAGC 
CAGATTTCTG CAGAGGACCT GCTCCGAATT GGAGTCACTC TGGCAGGACA CCAGAAGAAA 
ATCTTGGCCA GTGTCCAGCA TATGAAGTCC CAGGCCAAGC CAGGAGCCCC TGGTGGGACA 
GGAGCACCAG CTCAGCAATA CTGA