LOC101898570 cDNA ORF clone, Musca domestica(house fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101898570
Entrez Gene ID	101898570
Full Name	alanine--tRNA ligase, cytoplasmic
Gene Type	protein-coding
Organism	Musca domestica(house fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005190193.3	XP_005190250.2	alanine--tRNA ligase, cytoplasmic

Pathways from BioSystems
Gene Ontology

The following LOC101898570 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101898570 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMj51883	XM_005190193.3 Latest version!	Musca domestica alanine--tRNA ligase, cytoplasmic (LOC101898570), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101898570 ( XM_005190193.3 ) cDNA ORF clone, Musca domestica(house fly) -> XP_005190250.2 Musca domestica alanine--tRNA ligase, cytoplasmic (LOC101898570), mRNA.

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CloneID	OMj51883
Clone ID Related Accession (Same CDS sequence)	XM_005190193.3
Accession Version	XM_005190193.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-25
Organism	Musca domestica(house fly)
Product	alanine--tRNA ligase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004768080.1) and transcript sequence (GBAB01006074.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2017 this sequence version replaced XM_005190193.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Musca domestica Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1099 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCATTTTC TTACAGCTGC TGAAGTCCGC CAAGCCTACT TGGACTTTTT TAAGGAGAAA 
AATCATATTT ATGTGCATTC ATCCAGTACT ATTCCTTTGG ATGACCCAAC GCTGCTTTTC 
GCAAATGCTG GAATGAATCA GTTTAAACCT ATTTTCTTAG GAACAGCAGA TCCCAATAGT 
GACATGTCAA AGTGGCTTCG TGTAGCCAAT AGTCAAAAAT GTATTCGTGC AGGTGGTAAA 
CACAACGATT TAGACGATGT TGGTAAAGAT GTTTATCATC ACACGTTCTT CGAAATGTTG 
GGAAATTGGT CCTTTGGTGA TTATTTCAAA AAGGAAATAT GTGCCTGGGC TTGGGAGTTT 
TTAACGGAGC GATTAGGATT GGATAAGGAT CGATTGTATG TGACGTACTT TGGTGGAGAT 
GAAACAAGTG GTTTGGAGCC AGACTTAGAA TGCAAGCAAA TATGGATGAA TTTAGGATTA 
AAAGCTGAAC ATATCATACC AGGCAATATG AAAGATAACT TTTGGGAAAT GGGGGAGACG 
GGGCCTTGTG GTCCATGTTC CGAATTACAT TTTGATCGTA TAGGTGGTCG TAGTGTCCCC 
GAGCTTGTAA ACATGGATGA CCCCGATGTA TTGGAAATAT GGAATTTAGT CTTCATTCAA 
TTTAATCGAG AACAAGATGG GTCCTTGAAA CCATTGCCTA AAAAGCACAT TGACTGTGGT 
ATGGGCTTTG AGCGTTTGGT TTCAGTCATT CAAAATAAAC GTTCAAACTA CGACACTGAC 
ATATTTGTAC CTCTTTTTGA CGCTATTCAT GTAGGAACAG GATGTGCTCC ATATGGAGGT 
AAAGTTGGAG CAGAAGATGT TACCGGCGTA GATATGGCCT ATCGAGTTTT GGCTGATCAT 
GCAAGAACTC TAACCATTGC TTTAGCCGAT GGAGGAGTTC CCGACAATTC CGGCCGGGGT 
TATGTGCTAC GTCGAATTTT GCGCCGTGCC GTTAGGTATG CTACAGAAAA ATTAAACGCA 
AAACCAGGAT TTTTCGCGAC TCTCGTCTTT ACAGTCATTG ATTTACTTGG TGATGCTTTT 
CCTGAAGTAA AAAAGGATCC TCAGCATATT GTTGACATTA TAAACGAGGA GGAGCAACAA 
TTCTTAAAGA CACTTTCCAG AGGTAGAAAT CTCTTTAATA GGACTGTTGC CAAACTTGGA 
GATGAAAAAA TAATTCCAGG TGACGTCACT TGGCGTTTGT ATGACACATA TGGCTTTCCA 
GTAGATTTAA CTCAGTTAAT GGCCGAGGAA AGAGGTCTAC AAATTGATAT GGAGGGCTAT 
GAGTCCGCAA AACACAATTC CTATATTCTT TCTCAGGGAA AAGGTGCCAA CAAAGTTGAC 
GAGATCAATC TCGACGTTCA TGCCATTTCC CAACTTAAGG ATAAAGGAAT TCCAGTTACT 
GACGATAGCT TCAAATATTC ATATGATAGT GTATCAGATG ATCCTCAATC TGAATATAAA 
TTTAATTCGT GTACTGGAAA AATTGTTGCT ATACGATACA ATAATGAATT CGTACAAAAG 
GTTTATTCTG GTCAAAAGGC TGGAATTGTT TTAGATAAAA CTAATTTCTA TGCTGAAAGT 
GGAGGTCAAA TTTATGACCA AGGAGTTTTG GTAAAAGTGG ATGAAGAAGA AAATGAATTT 
ATGGTAGACT GTGTGTACAA CCGGGGCGGT TACATTCTTC ATATTGGGAT TGTGGAAGGC 
TGCCTTGCTG TTGGAAATAC GTTGAATTTA CAAATTGACA CAATTAGACG ATCGCTGACC 
ATGAAAAATC ATTCTGCTAC GCATGCCCTC AACCACAGCC TTTTACAAGT ACTGGGCAAA 
GACACCGATC AAAAGGGTTC CTTAGTAGTG CCAGAAAAAT TAAGATTTGA TTTTAGCTGC 
AAATCTGCCA TGACTACTGA ACAGATTGCA AAAACTGAAC AGTTAACAAA GGATCTTGTG 
TACCAGAACG TGCCCATCTA TGCAAAAGAA TCAAAATTGC ATGTTGCAAA AACCATCCGC 
GGGCTGCGCT CCGTTTTCGA TGAAGTATAC CCAGATCCAG TTAGAGTTAT ATCCTTTGGT 
ATCCCTGTTG AGGAATTAGA ACAGAATCCA GAACGTGATG CTGGAGAAAA AACTTCTGTA 
GAATTCTGCG GAGGAACTCA CTTAAAGCGG TCAGGCCACA TTGTCGATTT TGTAATAAGC 
AGTGAGGAAG CTATTTCAAA GGGCATCCGA CGCATTGTAG CTCTTACTGG GCCAGAAGCT 
TTGAAAGCTA TAAGTAAATC AAACCTATTT CAAGCTGAAA TCGAAAAACT TAACAAAGCA 
ATCGAAGAGG ATTCCCATGG TAAAAATTCT AAAAATATTG TTAAACAAAT TGTTGAAATT 
AATGAAGAAA TTTCTCGCGC CACTATTCCC CATGTTAAAA AGGATGAAAT GAGAAACAAT 
CTTAAAAATC TTAAGAAAAA ATTAGATGAT AAAGAGCGCG CATTGAAAAA TCTCGTTGCA 
CTACAAGTAG TAGAAAGGGT TAAAGAATTG TGCACTTCTA AACCGAATGC TCCGTATATG 
GTAGAACAAT TGGACGCCTA TAATAACACT AAGGCATTAG ACGCTGCTCT TAAACAAGTT 
CGTTCGTTAT GTCCAGACAC TTCTGCAATG TTTTTGTCTG TCGATGTTGA CTCCAAGAAA 
ATATTCTGTC TATCGTCTGT TCCCAAGACT GCTGTAGAAA AAGGCCTGAA AGCAAATGAA 
TGGGTACAAT ATATTTCTTC CGTTCTCAAC GGCAAAGGTG GCGGTAAGCC CGAATCTGCA 
CAAGCATCCG GCTCCAACTA CGAAAATATC GTAGACATAA TTAAAGTAGC TGAAGAATTC 
GCAAAAACTA AGCTTGAGGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005190250.2
CDS	105..3008
Translation	MHFLTAAEVRQAYLDFFKEKNHIYVHSSSTIPLDDPTLLFANAGMNQFKPIFLGTADPNSDMSKWLRVANSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICAWAWEFLTERLGLDKDRLYVTYFGGDETSGLEPDLECKQIWMNLGLKAEHIIPGNMKDNFWEMGETGPCGPCSELHFDRIGGRSVPELVNMDDPDVLEIWNLVFIQFNREQDGSLKPLPKKHIDCGMGFERLVSVIQNKRSNYDTDIFVPLFDAIHVGTGCAPYGGKVGAEDVTGVDMAYRVLADHARTLTIALADGGVPDNSGRGYVLRRILRRAVRYATEKLNAKPGFFATLVFTVIDLLGDAFPEVKKDPQHIVDIINEEEQQFLKTLSRGRNLFNRTVAKLGDEKIIPGDVTWRLYDTYGFPVDLTQLMAEERGLQIDMEGYESAKHNSYILSQGKGANKVDEINLDVHAISQLKDKGIPVTDDSFKYSYDSVSDDPQSEYKFNSCTGKIVAIRYNNEFVQKVYSGQKAGIVLDKTNFYAESGGQIYDQGVLVKVDEEENEFMVDCVYNRGGYILHIGIVEGCLAVGNTLNLQIDTIRRSLTMKNHSATHALNHSLLQVLGKDTDQKGSLVVPEKLRFDFSCKSAMTTEQIAKTEQLTKDLVYQNVPIYAKESKLHVAKTIRGLRSVFDEVYPDPVRVISFGIPVEELEQNPERDAGEKTSVEFCGGTHLKRSGHIVDFVISSEEAISKGIRRIVALTGPEALKAISKSNLFQAEIEKLNKAIEEDSHGKNSKNIVKQIVEINEEISRATIPHVKKDEMRNNLKNLKKKLDDKERALKNLVALQVVERVKELCTSKPNAPYMVEQLDAYNNTKALDAALKQVRSLCPDTSAMFLSVDVDSKKIFCLSSVPKTAVEKGLKANEWVQYISSVLNGKGGGKPESAQASGSNYENIVDIIKVAEEFAKTKLEA

Target ORF information:

RefSeq Version	XM_005190193.3
Organism	Musca domestica(house fly)
Definition	Musca domestica alanine--tRNA ligase, cytoplasmic (LOC101898570), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005190193.3

ORF Insert Sequence:

ATGCATTTTC TTACAGCTGC TGAAGTCCGC CAAGCCTACT TGGACTTTTT TAAGGAGAAA 
AATCATATTT ATGTGCATTC ATCCAGTACT ATTCCTTTGG ATGACCCAAC GCTGCTTTTC 
GCAAATGCTG GAATGAATCA GTTTAAACCT ATTTTCTTAG GAACAGCAGA TCCCAATAGT 
GACATGTCAA AGTGGCTTCG TGTAGCCAAT AGTCAAAAAT GTATTCGTGC AGGTGGTAAA 
CACAACGATT TAGACGATGT TGGTAAAGAT GTTTATCATC ACACGTTCTT CGAAATGTTG 
GGAAATTGGT CCTTTGGTGA TTATTTCAAA AAGGAAATAT GTGCCTGGGC TTGGGAGTTT 
TTAACGGAGC GATTAGGATT GGATAAGGAT CGATTGTATG TGACGTACTT TGGTGGAGAT 
GAAACAAGTG GTTTGGAGCC AGACTTAGAA TGCAAGCAAA TATGGATGAA TTTAGGATTA 
AAAGCTGAAC ATATCATACC AGGCAATATG AAAGATAACT TTTGGGAAAT GGGGGAGACG 
GGGCCTTGTG GTCCATGTTC CGAATTACAT TTTGATCGTA TAGGTGGTCG TAGTGTCCCC 
GAGCTTGTAA ACATGGATGA CCCCGATGTA TTGGAAATAT GGAATTTAGT CTTCATTCAA 
TTTAATCGAG AACAAGATGG GTCCTTGAAA CCATTGCCTA AAAAGCACAT TGACTGTGGT 
ATGGGCTTTG AGCGTTTGGT TTCAGTCATT CAAAATAAAC GTTCAAACTA CGACACTGAC 
ATATTTGTAC CTCTTTTTGA CGCTATTCAT GTAGGAACAG GATGTGCTCC ATATGGAGGT 
AAAGTTGGAG CAGAAGATGT TACCGGCGTA GATATGGCCT ATCGAGTTTT GGCTGATCAT 
GCAAGAACTC TAACCATTGC TTTAGCCGAT GGAGGAGTTC CCGACAATTC CGGCCGGGGT 
TATGTGCTAC GTCGAATTTT GCGCCGTGCC GTTAGGTATG CTACAGAAAA ATTAAACGCA 
AAACCAGGAT TTTTCGCGAC TCTCGTCTTT ACAGTCATTG ATTTACTTGG TGATGCTTTT 
CCTGAAGTAA AAAAGGATCC TCAGCATATT GTTGACATTA TAAACGAGGA GGAGCAACAA 
TTCTTAAAGA CACTTTCCAG AGGTAGAAAT CTCTTTAATA GGACTGTTGC CAAACTTGGA 
GATGAAAAAA TAATTCCAGG TGACGTCACT TGGCGTTTGT ATGACACATA TGGCTTTCCA 
GTAGATTTAA CTCAGTTAAT GGCCGAGGAA AGAGGTCTAC AAATTGATAT GGAGGGCTAT 
GAGTCCGCAA AACACAATTC CTATATTCTT TCTCAGGGAA AAGGTGCCAA CAAAGTTGAC 
GAGATCAATC TCGACGTTCA TGCCATTTCC CAACTTAAGG ATAAAGGAAT TCCAGTTACT 
GACGATAGCT TCAAATATTC ATATGATAGT GTATCAGATG ATCCTCAATC TGAATATAAA 
TTTAATTCGT GTACTGGAAA AATTGTTGCT ATACGATACA ATAATGAATT CGTACAAAAG 
GTTTATTCTG GTCAAAAGGC TGGAATTGTT TTAGATAAAA CTAATTTCTA TGCTGAAAGT 
GGAGGTCAAA TTTATGACCA AGGAGTTTTG GTAAAAGTGG ATGAAGAAGA AAATGAATTT 
ATGGTAGACT GTGTGTACAA CCGGGGCGGT TACATTCTTC ATATTGGGAT TGTGGAAGGC 
TGCCTTGCTG TTGGAAATAC GTTGAATTTA CAAATTGACA CAATTAGACG ATCGCTGACC 
ATGAAAAATC ATTCTGCTAC GCATGCCCTC AACCACAGCC TTTTACAAGT ACTGGGCAAA 
GACACCGATC AAAAGGGTTC CTTAGTAGTG CCAGAAAAAT TAAGATTTGA TTTTAGCTGC 
AAATCTGCCA TGACTACTGA ACAGATTGCA AAAACTGAAC AGTTAACAAA GGATCTTGTG 
TACCAGAACG TGCCCATCTA TGCAAAAGAA TCAAAATTGC ATGTTGCAAA AACCATCCGC 
GGGCTGCGCT CCGTTTTCGA TGAAGTATAC CCAGATCCAG TTAGAGTTAT ATCCTTTGGT 
ATCCCTGTTG AGGAATTAGA ACAGAATCCA GAACGTGATG CTGGAGAAAA AACTTCTGTA 
GAATTCTGCG GAGGAACTCA CTTAAAGCGG TCAGGCCACA TTGTCGATTT TGTAATAAGC 
AGTGAGGAAG CTATTTCAAA GGGCATCCGA CGCATTGTAG CTCTTACTGG GCCAGAAGCT 
TTGAAAGCTA TAAGTAAATC AAACCTATTT CAAGCTGAAA TCGAAAAACT TAACAAAGCA 
ATCGAAGAGG ATTCCCATGG TAAAAATTCT AAAAATATTG TTAAACAAAT TGTTGAAATT 
AATGAAGAAA TTTCTCGCGC CACTATTCCC CATGTTAAAA AGGATGAAAT GAGAAACAAT 
CTTAAAAATC TTAAGAAAAA ATTAGATGAT AAAGAGCGCG CATTGAAAAA TCTCGTTGCA 
CTACAAGTAG TAGAAAGGGT TAAAGAATTG TGCACTTCTA AACCGAATGC TCCGTATATG 
GTAGAACAAT TGGACGCCTA TAATAACACT AAGGCATTAG ACGCTGCTCT TAAACAAGTT 
CGTTCGTTAT GTCCAGACAC TTCTGCAATG TTTTTGTCTG TCGATGTTGA CTCCAAGAAA 
ATATTCTGTC TATCGTCTGT TCCCAAGACT GCTGTAGAAA AAGGCCTGAA AGCAAATGAA 
TGGGTACAAT ATATTTCTTC CGTTCTCAAC GGCAAAGGTG GCGGTAAGCC CGAATCTGCA 
CAAGCATCCG GCTCCAACTA CGAAAATATC GTAGACATAA TTAAAGTAGC TGAAGAATTC 
GCAAAAACTA AGCTTGAGGC TTAA