Cul4a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cul4a
Entrez Gene ID	99375
Full Name	cullin 4A
Synonyms	2810470J21Rik,AW495282
General protein information	Preferred Names cullin 4A Names cullin-4A CUL-4A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 A1.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_146207.2	NP_666319.2	cullin-4A
XM_006508933.1	XP_006508996.1	cullin-4A isoform X1
XM_006508935.3	XP_006508998.1	cullin-4A isoform X2
NM_001363450.1	NP_001350379.1	cullin-4A isoform 3
NM_146207.3	NP_666319.2	cullin-4A isoform 1
NM_001363448.1	NP_001350377.1	cullin-4A isoform 2

Pathways from BioSystems

Homologies

	LOC714302	XP_002808198.1
Rattus norvegicus (Norway rat)	Cul4a	NP_001120773.1
Canis lupus familiaris (dog)	CUL4A	XP_849542.3
Bos taurus (cattle)	CUL4A	XP_002692061.2
Homo sapiens (human)	CUL4A	NP_001008895.1
Pan troglodytes (chimpanzee)	CUL4A	XP_003314265.1
Mus musculus (house mouse)	Cul4a	NP_666319.2
Gallus gallus (chicken)	CUL4A	XP_003640577.1
Xenopus tropicalis (tropical clawed frog)	cul4a	NP_001015893.1

Gene Ontology
Bibliography

Related articles in PubMed

CRL4
Li G, Ji T, Chen J, Fu Y, Hou L, Feng Y, Zhang T, Song T, Zhao J, Endo Y, Lin H, Cai X, Cang Y
Cell reports18(6)1499-1511(2017 Feb)

Genome-wide RNAi Screening Identifies Protein Modules Required for 40S Subunit Synthesis in Human Cells.
Badertscher L, Wild T, Montellese C, Alexander LT, Bammert L, Sarazova M, Stebler M, Csucs G, Mayer TU, Zamboni N, Zemp I, Horvath P, Kutay U
Cell reports13(12)2879-91(2015 Dec)

CUL4A facilitates hepatocarcinogenesis by promoting cell cycle progression and epithelial-mesenchymal transition.
Pan Y, Wang B, Yang X, Bai F, Xu Q, Li X, Gao L, Ma C, Liang X
Scientific reports517006(2015 Nov)

The CUL4-DDB1 ubiquitin ligase complex controls adult and embryonic stem cell differentiation and homeostasis.
Gao J, Buckley SM, Cimmino L, Guillamot M, Strikoudis A, Cang Y, Goff SP, Aifantis I
eLife4(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CUL4A facilitates hepatocarcinogenesis by promoting cell cycle progression and epithelial-mesenchymal transition.
Title: CUL4A facilitates hepatocarcinogenesis by promoting cell cycle progression and epithelial-mesenchymal transition.

These studies identify CUL4-DDB1 complex as a novel post-translational regulator of stem and progenitor maintenance and differentiation.
Title: The CUL4-DDB1 ubiquitin ligase complex controls adult and embryonic stem cell differentiation and homeostasis.

Findings indicate that Cul4A is oncogenic in vivo and that Cul4A over-expression is associated with cisplatin resistance in lung cancer cells.
Title: Lung tumourigenesis in a conditional Cul4A transgenic mouse model.

this study revealed an indispensable role for Cul4a during male germ cell meiosis.
Title: The E3 ubiquitin ligase Cullin 4A regulates meiotic progression in mouse spermatogenesis.

The primary spermatocytes in Cul4A-/- mice are deficient in progression through late prophase I, a time point when expression of the X-linked Cul4B gene is silenced due to meiotic sex chromosome inactivation.
Title: Cul4A is essential for spermatogenesis and male fertility.

The following Cul4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cul4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu40504	NM_001363450.1 Latest version!	Mus musculus cullin 4A (Cul4a), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu40504	XM_006508935.3 Latest version!	Mus musculus cullin 4A (Cul4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu22530	NM_146207.3 Latest version!	Mus musculus cullin 4A (Cul4a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$447.30 $639.00
OMu40503	NM_001363448.1 Latest version!	Mus musculus cullin 4A (Cul4a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu40503	XM_006508933.1 Latest version!	Mus musculus cullin 4A (Cul4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OMu22530	NM_146207.2	Mus musculus cullin 4A (Cul4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$447.30 $639.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cul4a ( NM_001363450.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001350379.1 Mus musculus cullin 4A (Cul4a), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu40504
Clone ID Related Accession (Same CDS sequence)	XM_006508935.3 , NM_001363450.1
Accession Version	NM_001363450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	cullin-4A isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC133520.4. On May 18, 2018 this sequence version replaced XM_006508935.3. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCATGA TCAGAAGCAT CTTCCTGTTT CTGGATCGAA CCTACGTCCT CCAGAATTCC 
ATGCTGCCTT CTATCTGGGA CATGGGCCTA GAGCTGTTTA GGAACCACAT CATCAGCGAC 
AGGATGGTGC AGAGTAAGAC CATTGATGGG ATCCTGCTGC TGATTGGGCG TGAACGAAGT 
GGAGAGGCTG TGGACAGGAG TCTGCTTCGG AGTTTGCTAA GCATGCTCTC AGATCTCCAG 
GTGTACAAAG ACTCATTTGA GCTGAAGTTC TTGGAAGAGA CTAACTGTCT ATACGCTGCT 
GAAGGCCAGA GGCTGATGCA GGACAGGGAG GTTCCAGAAT ACCTTAACCA TGTGAGCAAA 
CGTCTAGAAG AGGAAGCAGA CCGAGTGATC ACCTACTTAG ACCACAGCAC ACAAAAACCG 
TTGATTGCCT GTGTGGAGAA ACAGCTGTTA GGAGAACATT TGACAGCAAT TCTACAGAAA 
GGCCTAGAGC ACCTGCTGGA TGAGAACAGG GTGCCCGACC TCACCCAGAT GTACCAGCTC 
TTCAGCCGGG TGAAAGGAGG GCAGCATGCG CTGCTGCAGC ACTGGAGCGA GTACATCAAG 
ACCTTTGGAA CAACCATTGT CATCAATCCT GAGAAAGACA AAGACATGGT CCAAGACCTG 
CTGGACTTTA AGGACAAAGT GGACCACGTG GTGGAGGTGT GCTTCCAGAG GAACGAGCGC 
TTCATCAACC TGATGAAGGA GTCCTTCGAG ACGTTCATCA ACAAGAGACC AAACAAGCCT 
GCAGAGCTCA TTGCCAAGCA CGTGGACTCA AAGTTACGGG CCGGCAACAA AGAAGCCACA 
GATGAGGAAC TGGAGAGGAT CCTGGACAAG ATCATGATTC TCTTCCGTTT CATCCATGGT 
AAAGATGTCT TTGAAGCGTT TTATAAGAAA GACTTAGCAA AAAGGCTGCT GGTGGGGAAG 
AGCGCGTCGG TGGACGCGGA GAAGTCCATG CTGTCGAAGC TCAAGCACGA GTGCGGAGCT 
GCGTTTACCA GCAAACTGGA GGGCATGTTC AAGGACATGG AGCTCTCCAA AGACATCATG 
GTTCACTTCA AGCAGCATAT GCAGAACCAG AGTGCGCCAG GCCCCATTGA CCTCACGGTG 
AACATACTCA CCATGGGCTA CTGGCCCACG TACACACCCA TGGAAGTGCA CCTCCCTCCA 
GAGATGGTCA GACTTCAGGA AGTATTCAAG ACGTTCTACC TGGGCAAGCA CAGTGGGCGG 
AAGCTGCAAT GGCAGACAAC GCTGGGACAC GCCGTGCTGA AGGCGGACTT TAAGGAGGGG 
AAGAAGGAGT TCCAGGTATC CCTCTTCCAG ACGCTGGTGC TGCTCATGTT CAACGAAGGG 
GATGGATTTA GCTTTGAAGA AATAAAAATG GCCACTGGCA TAGAGGACAG TGAGTTGCGA 
AGAACACTAC AGTCTCTGGC CTGTGGTAAA GCACGGGTCC TAATTAAAAG TCCCAAAGGA 
AAGGAGGTGG AAGATGGAGA TAAGTTCATC TTCAATGCAG ACTTCAAACA CAAGTTGTTC 
AGAATAAAGA TCAATCAAAT CCAGATGAAG GAGACCGTTG AAGAACAGGT TAGCACTACA 
GAGAGAGTAT TCCAGGATAG ACAGTACCAG ATCGACGCTG CTATAGTCAG AATAATGAAA 
ATGAGGAAGA CACTTGGTCA TAATCTTCTA GTTTCTGAAT TATACAATCA GCTGAAATTT 
CCAGTGAAGC CTGGAGATTT GAAAAAAAGG ATTGAGTCAC TTATAGACAG AGACTATATG 
GAACGAGACA AAGACAGTCC AAATCAGTAC CACTACGTGG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350379.1
CDS	453..2297
Translation	MIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRNHIISDRMVQSKTIDGILLLIGRERSGEAVDRSLLRSLLSMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQDREVPEYLNHVSKRLEEEADRVITYLDHSTQKPLIACVEKQLLGEHLTAILQKGLEHLLDENRVPDLTQMYQLFSRVKGGQHALLQHWSEYIKTFGTTIVINPEKDKDMVQDLLDFKDKVDHVVEVCFQRNERFINLMKESFETFINKRPNKPAELIAKHVDSKLRAGNKEATDEELERILDKIMILFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHFKQHMQNQSAPGPIDLTVNILTMGYWPTYTPMEVHLPPEMVRLQEVFKTFYLGKHSGRKLQWQTTLGHAVLKADFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNADFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDSPNQYHYVA

Target ORF information:

RefSeq Version	NM_001363450.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cullin 4A (Cul4a), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363450.1

ORF Insert Sequence:

ATGATCATGA TCAGAAGCAT CTTCCTGTTT CTGGATCGAA CCTACGTCCT CCAGAATTCC 
ATGCTGCCTT CTATCTGGGA CATGGGCCTA GAGCTGTTTA GGAACCACAT CATCAGCGAC 
AGGATGGTGC AGAGTAAGAC CATTGATGGG ATCCTGCTGC TGATTGGGCG TGAACGAAGT 
GGAGAGGCTG TGGACAGGAG TCTGCTTCGG AGTTTGCTAA GCATGCTCTC AGATCTCCAG 
GTGTACAAAG ACTCATTTGA GCTGAAGTTC TTGGAAGAGA CTAACTGTCT ATACGCTGCT 
GAAGGCCAGA GGCTGATGCA GGACAGGGAG GTTCCAGAAT ACCTTAACCA TGTGAGCAAA 
CGTCTAGAAG AGGAAGCAGA CCGAGTGATC ACCTACTTAG ACCACAGCAC ACAAAAACCG 
TTGATTGCCT GTGTGGAGAA ACAGCTGTTA GGAGAACATT TGACAGCAAT TCTACAGAAA 
GGCCTAGAGC ACCTGCTGGA TGAGAACAGG GTGCCCGACC TCACCCAGAT GTACCAGCTC 
TTCAGCCGGG TGAAAGGAGG GCAGCATGCG CTGCTGCAGC ACTGGAGCGA GTACATCAAG 
ACCTTTGGAA CAACCATTGT CATCAATCCT GAGAAAGACA AAGACATGGT CCAAGACCTG 
CTGGACTTTA AGGACAAAGT GGACCACGTG GTGGAGGTGT GCTTCCAGAG GAACGAGCGC 
TTCATCAACC TGATGAAGGA GTCCTTCGAG ACGTTCATCA ACAAGAGACC AAACAAGCCT 
GCAGAGCTCA TTGCCAAGCA CGTGGACTCA AAGTTACGGG CCGGCAACAA AGAAGCCACA 
GATGAGGAAC TGGAGAGGAT CCTGGACAAG ATCATGATTC TCTTCCGTTT CATCCATGGT 
AAAGATGTCT TTGAAGCGTT TTATAAGAAA GACTTAGCAA AAAGGCTGCT GGTGGGGAAG 
AGCGCGTCGG TGGACGCGGA GAAGTCCATG CTGTCGAAGC TCAAGCACGA GTGCGGAGCT 
GCGTTTACCA GCAAACTGGA GGGCATGTTC AAGGACATGG AGCTCTCCAA AGACATCATG 
GTTCACTTCA AGCAGCATAT GCAGAACCAG AGTGCGCCAG GCCCCATTGA CCTCACGGTG 
AACATACTCA CCATGGGCTA CTGGCCCACG TACACACCCA TGGAAGTGCA CCTCCCTCCA 
GAGATGGTCA GACTTCAGGA AGTATTCAAG ACGTTCTACC TGGGCAAGCA CAGTGGGCGG 
AAGCTGCAAT GGCAGACAAC GCTGGGACAC GCCGTGCTGA AGGCGGACTT TAAGGAGGGG 
AAGAAGGAGT TCCAGGTATC CCTCTTCCAG ACGCTGGTGC TGCTCATGTT CAACGAAGGG 
GATGGATTTA GCTTTGAAGA AATAAAAATG GCCACTGGCA TAGAGGACAG TGAGTTGCGA 
AGAACACTAC AGTCTCTGGC CTGTGGTAAA GCACGGGTCC TAATTAAAAG TCCCAAAGGA 
AAGGAGGTGG AAGATGGAGA TAAGTTCATC TTCAATGCAG ACTTCAAACA CAAGTTGTTC 
AGAATAAAGA TCAATCAAAT CCAGATGAAG GAGACCGTTG AAGAACAGGT TAGCACTACA 
GAGAGAGTAT TCCAGGATAG ACAGTACCAG ATCGACGCTG CTATAGTCAG AATAATGAAA 
ATGAGGAAGA CACTTGGTCA TAATCTTCTA GTTTCTGAAT TATACAATCA GCTGAAATTT 
CCAGTGAAGC CTGGAGATTT GAAAAAAAGG ATTGAGTCAC TTATAGACAG AGACTATATG 
GAACGAGACA AAGACAGTCC AAATCAGTAC CACTACGTGG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul4a ( XM_006508935.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508998.1 Mus musculus cullin 4A (Cul4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu40504
Clone ID Related Accession (Same CDS sequence)	XM_006508935.3 , NM_001363450.1
Accession Version	XM_006508935.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cullin-4A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508935.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCATGA TCAGAAGCAT CTTCCTGTTT CTGGATCGAA CCTACGTCCT CCAGAATTCC 
ATGCTGCCTT CTATCTGGGA CATGGGCCTA GAGCTGTTTA GGAACCACAT CATCAGCGAC 
AGGATGGTGC AGAGTAAGAC CATTGATGGG ATCCTGCTGC TGATTGGGCG TGAACGAAGT 
GGAGAGGCTG TGGACAGGAG TCTGCTTCGG AGTTTGCTAA GCATGCTCTC AGATCTCCAG 
GTGTACAAAG ACTCATTTGA GCTGAAGTTC TTGGAAGAGA CTAACTGTCT ATACGCTGCT 
GAAGGCCAGA GGCTGATGCA GGACAGGGAG GTTCCAGAAT ACCTTAACCA TGTGAGCAAA 
CGTCTAGAAG AGGAAGCAGA CCGAGTGATC ACCTACTTAG ACCACAGCAC ACAAAAACCG 
TTGATTGCCT GTGTGGAGAA ACAGCTGTTA GGAGAACATT TGACAGCAAT TCTACAGAAA 
GGCCTAGAGC ACCTGCTGGA TGAGAACAGG GTGCCCGACC TCACCCAGAT GTACCAGCTC 
TTCAGCCGGG TGAAAGGAGG GCAGCATGCG CTGCTGCAGC ACTGGAGCGA GTACATCAAG 
ACCTTTGGAA CAACCATTGT CATCAATCCT GAGAAAGACA AAGACATGGT CCAAGACCTG 
CTGGACTTTA AGGACAAAGT GGACCACGTG GTGGAGGTGT GCTTCCAGAG GAACGAGCGC 
TTCATCAACC TGATGAAGGA GTCCTTCGAG ACGTTCATCA ACAAGAGACC AAACAAGCCT 
GCAGAGCTCA TTGCCAAGCA CGTGGACTCA AAGTTACGGG CCGGCAACAA AGAAGCCACA 
GATGAGGAAC TGGAGAGGAT CCTGGACAAG ATCATGATTC TCTTCCGTTT CATCCATGGT 
AAAGATGTCT TTGAAGCGTT TTATAAGAAA GACTTAGCAA AAAGGCTGCT GGTGGGGAAG 
AGCGCGTCGG TGGACGCGGA GAAGTCCATG CTGTCGAAGC TCAAGCACGA GTGCGGAGCT 
GCGTTTACCA GCAAACTGGA GGGCATGTTC AAGGACATGG AGCTCTCCAA AGACATCATG 
GTTCACTTCA AGCAGCATAT GCAGAACCAG AGTGCGCCAG GCCCCATTGA CCTCACGGTG 
AACATACTCA CCATGGGCTA CTGGCCCACG TACACACCCA TGGAAGTGCA CCTCCCTCCA 
GAGATGGTCA GACTTCAGGA AGTATTCAAG ACGTTCTACC TGGGCAAGCA CAGTGGGCGG 
AAGCTGCAAT GGCAGACAAC GCTGGGACAC GCCGTGCTGA AGGCGGACTT TAAGGAGGGG 
AAGAAGGAGT TCCAGGTATC CCTCTTCCAG ACGCTGGTGC TGCTCATGTT CAACGAAGGG 
GATGGATTTA GCTTTGAAGA AATAAAAATG GCCACTGGCA TAGAGGACAG TGAGTTGCGA 
AGAACACTAC AGTCTCTGGC CTGTGGTAAA GCACGGGTCC TAATTAAAAG TCCCAAAGGA 
AAGGAGGTGG AAGATGGAGA TAAGTTCATC TTCAATGCAG ACTTCAAACA CAAGTTGTTC 
AGAATAAAGA TCAATCAAAT CCAGATGAAG GAGACCGTTG AAGAACAGGT TAGCACTACA 
GAGAGAGTAT TCCAGGATAG ACAGTACCAG ATCGACGCTG CTATAGTCAG AATAATGAAA 
ATGAGGAAGA CACTTGGTCA TAATCTTCTA GTTTCTGAAT TATACAATCA GCTGAAATTT 
CCAGTGAAGC CTGGAGATTT GAAAAAAAGG ATTGAGTCAC TTATAGACAG AGACTATATG 
GAACGAGACA AAGACAGTCC AAATCAGTAC CACTACGTGG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508998.1
CDS	556..2400
Translation	MIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRNHIISDRMVQSKTIDGILLLIGRERSGEAVDRSLLRSLLSMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQDREVPEYLNHVSKRLEEEADRVITYLDHSTQKPLIACVEKQLLGEHLTAILQKGLEHLLDENRVPDLTQMYQLFSRVKGGQHALLQHWSEYIKTFGTTIVINPEKDKDMVQDLLDFKDKVDHVVEVCFQRNERFINLMKESFETFINKRPNKPAELIAKHVDSKLRAGNKEATDEELERILDKIMILFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHFKQHMQNQSAPGPIDLTVNILTMGYWPTYTPMEVHLPPEMVRLQEVFKTFYLGKHSGRKLQWQTTLGHAVLKADFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNADFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDSPNQYHYVA

Target ORF information:

RefSeq Version	XM_006508935.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cullin 4A (Cul4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508935.3

ORF Insert Sequence:

ATGATCATGA TCAGAAGCAT CTTCCTGTTT CTGGATCGAA CCTACGTCCT CCAGAATTCC 
ATGCTGCCTT CTATCTGGGA CATGGGCCTA GAGCTGTTTA GGAACCACAT CATCAGCGAC 
AGGATGGTGC AGAGTAAGAC CATTGATGGG ATCCTGCTGC TGATTGGGCG TGAACGAAGT 
GGAGAGGCTG TGGACAGGAG TCTGCTTCGG AGTTTGCTAA GCATGCTCTC AGATCTCCAG 
GTGTACAAAG ACTCATTTGA GCTGAAGTTC TTGGAAGAGA CTAACTGTCT ATACGCTGCT 
GAAGGCCAGA GGCTGATGCA GGACAGGGAG GTTCCAGAAT ACCTTAACCA TGTGAGCAAA 
CGTCTAGAAG AGGAAGCAGA CCGAGTGATC ACCTACTTAG ACCACAGCAC ACAAAAACCG 
TTGATTGCCT GTGTGGAGAA ACAGCTGTTA GGAGAACATT TGACAGCAAT TCTACAGAAA 
GGCCTAGAGC ACCTGCTGGA TGAGAACAGG GTGCCCGACC TCACCCAGAT GTACCAGCTC 
TTCAGCCGGG TGAAAGGAGG GCAGCATGCG CTGCTGCAGC ACTGGAGCGA GTACATCAAG 
ACCTTTGGAA CAACCATTGT CATCAATCCT GAGAAAGACA AAGACATGGT CCAAGACCTG 
CTGGACTTTA AGGACAAAGT GGACCACGTG GTGGAGGTGT GCTTCCAGAG GAACGAGCGC 
TTCATCAACC TGATGAAGGA GTCCTTCGAG ACGTTCATCA ACAAGAGACC AAACAAGCCT 
GCAGAGCTCA TTGCCAAGCA CGTGGACTCA AAGTTACGGG CCGGCAACAA AGAAGCCACA 
GATGAGGAAC TGGAGAGGAT CCTGGACAAG ATCATGATTC TCTTCCGTTT CATCCATGGT 
AAAGATGTCT TTGAAGCGTT TTATAAGAAA GACTTAGCAA AAAGGCTGCT GGTGGGGAAG 
AGCGCGTCGG TGGACGCGGA GAAGTCCATG CTGTCGAAGC TCAAGCACGA GTGCGGAGCT 
GCGTTTACCA GCAAACTGGA GGGCATGTTC AAGGACATGG AGCTCTCCAA AGACATCATG 
GTTCACTTCA AGCAGCATAT GCAGAACCAG AGTGCGCCAG GCCCCATTGA CCTCACGGTG 
AACATACTCA CCATGGGCTA CTGGCCCACG TACACACCCA TGGAAGTGCA CCTCCCTCCA 
GAGATGGTCA GACTTCAGGA AGTATTCAAG ACGTTCTACC TGGGCAAGCA CAGTGGGCGG 
AAGCTGCAAT GGCAGACAAC GCTGGGACAC GCCGTGCTGA AGGCGGACTT TAAGGAGGGG 
AAGAAGGAGT TCCAGGTATC CCTCTTCCAG ACGCTGGTGC TGCTCATGTT CAACGAAGGG 
GATGGATTTA GCTTTGAAGA AATAAAAATG GCCACTGGCA TAGAGGACAG TGAGTTGCGA 
AGAACACTAC AGTCTCTGGC CTGTGGTAAA GCACGGGTCC TAATTAAAAG TCCCAAAGGA 
AAGGAGGTGG AAGATGGAGA TAAGTTCATC TTCAATGCAG ACTTCAAACA CAAGTTGTTC 
AGAATAAAGA TCAATCAAAT CCAGATGAAG GAGACCGTTG AAGAACAGGT TAGCACTACA 
GAGAGAGTAT TCCAGGATAG ACAGTACCAG ATCGACGCTG CTATAGTCAG AATAATGAAA 
ATGAGGAAGA CACTTGGTCA TAATCTTCTA GTTTCTGAAT TATACAATCA GCTGAAATTT 
CCAGTGAAGC CTGGAGATTT GAAAAAAAGG ATTGAGTCAC TTATAGACAG AGACTATATG 
GAACGAGACA AAGACAGTCC AAATCAGTAC CACTACGTGG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul4a ( NM_146207.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666319.2 Mus musculus cullin 4A (Cul4a), mRNA.

Price & Availability↑

CloneID	OMu22530
Clone ID Related Accession (Same CDS sequence)	NM_146207.2 , NM_146207.3
Accession Version	NM_146207.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-29
Organism	Mus musculus(house mouse)
Product	cullin-4A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC133520.4. On Feb 8, 2008 this sequence version replaced NM_146207.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.152470.1, SRR1660817.84716.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG AGGGCCCTCG GAAGGGCAGC GTCTCGGCTC TCATGGGCCG CACCAACGGC 
CTCACCAAGC CCGCGGCGTT GGCCGGTGGC CCCGCCAAGC CGGGGGGCAC GGGAGGCTCC 
AGGAAGCTGG TCATCAAGAA CTTCCGAGAC AGGCCGCGGC TGCCTGACAA CTACACTCAG 
GACACGTGGC GGAAGCTTCA CGAAGCGGTC AAGGCCATCC AGAGCAGCAC ATCCATCAGG 
TACAACCTGG AGGAGCTGTA CCAGGCTGTT GAAAATCTTT GTTCTCACAA AGTCTCCCCA 
ACGCTCTACA AGCAGCTGCG CCAGGTCTGT GAAGACCATG TCCAGGCCCA GATCCTCCCA 
TTCAGAGAAG ACTCACTAGA CAGCGTTTTA TTTCTAAAGA AGATTAACAC ATGCTGGCAA 
GATCACTGCA GACAAATGAT CATGATCAGA AGCATCTTCC TGTTTCTGGA TCGAACCTAC 
GTCCTCCAGA ATTCCATGCT GCCTTCTATC TGGGACATGG GCCTAGAGCT GTTTAGGAAC 
CACATCATCA GCGACAGGAT GGTGCAGAGT AAGACCATTG ATGGGATCCT GCTGCTGATT 
GGGCGTGAAC GAAGTGGAGA GGCTGTGGAC AGGAGTCTGC TTCGGAGTTT GCTAAGCATG 
CTCTCAGATC TCCAGGTGTA CAAAGACTCA TTTGAGCTGA AGTTCTTGGA AGAGACTAAC 
TGTCTATACG CTGCTGAAGG CCAGAGGCTG ATGCAGGACA GGGAGGTTCC AGAATACCTT 
AACCATGTGA GCAAACGTCT AGAAGAGGAA GCAGACCGAG TGATCACCTA CTTAGACCAC 
AGCACACAAA AACCGTTGAT TGCCTGTGTG GAGAAACAGC TGTTAGGAGA ACATTTGACA 
GCAATTCTAC AGAAAGGCCT AGAGCACCTG CTGGATGAGA ACAGGGTGCC CGACCTCACC 
CAGATGTACC AGCTCTTCAG CCGGGTGAAA GGAGGGCAGC ATGCGCTGCT GCAGCACTGG 
AGCGAGTACA TCAAGACCTT TGGAACAACC ATTGTCATCA ATCCTGAGAA AGACAAAGAC 
ATGGTCCAAG ACCTGCTGGA CTTTAAGGAC AAAGTGGACC ACGTGGTGGA GGTGTGCTTC 
CAGAGGAACG AGCGCTTCAT CAACCTGATG AAGGAGTCCT TCGAGACGTT CATCAACAAG 
AGACCAAACA AGCCTGCAGA GCTCATTGCC AAGCACGTGG ACTCAAAGTT ACGGGCCGGC 
AACAAAGAAG CCACAGATGA GGAACTGGAG AGGATCCTGG ACAAGATCAT GATTCTCTTC 
CGTTTCATCC ATGGTAAAGA TGTCTTTGAA GCGTTTTATA AGAAAGACTT AGCAAAAAGG 
CTGCTGGTGG GGAAGAGCGC GTCGGTGGAC GCGGAGAAGT CCATGCTGTC GAAGCTCAAG 
CACGAGTGCG GAGCTGCGTT TACCAGCAAA CTGGAGGGCA TGTTCAAGGA CATGGAGCTC 
TCCAAAGACA TCATGGTTCA CTTCAAGCAG CATATGCAGA ACCAGAGTGC GCCAGGCCCC 
ATTGACCTCA CGGTGAACAT ACTCACCATG GGCTACTGGC CCACGTACAC ACCCATGGAA 
GTGCACCTCC CTCCAGAGAT GGTCAGACTT CAGGAAGTAT TCAAGACGTT CTACCTGGGC 
AAGCACAGTG GGCGGAAGCT GCAATGGCAG ACAACGCTGG GACACGCCGT GCTGAAGGCG 
GACTTTAAGG AGGGGAAGAA GGAGTTCCAG GTATCCCTCT TCCAGACGCT GGTGCTGCTC 
ATGTTCAACG AAGGGGATGG ATTTAGCTTT GAAGAAATAA AAATGGCCAC TGGCATAGAG 
GACAGTGAGT TGCGAAGAAC ACTACAGTCT CTGGCCTGTG GTAAAGCACG GGTCCTAATT 
AAAAGTCCCA AAGGAAAGGA GGTGGAAGAT GGAGATAAGT TCATCTTCAA TGCAGACTTC 
AAACACAAGT TGTTCAGAAT AAAGATCAAT CAAATCCAGA TGAAGGAGAC CGTTGAAGAA 
CAGGTTAGCA CTACAGAGAG AGTATTCCAG GATAGACAGT ACCAGATCGA CGCTGCTATA 
GTCAGAATAA TGAAAATGAG GAAGACACTT GGTCATAATC TTCTAGTTTC TGAATTATAC 
AATCAGCTGA AATTTCCAGT GAAGCCTGGA GATTTGAAAA AAAGGATTGA GTCACTTATA 
GACAGAGACT ATATGGAACG AGACAAAGAC AGTCCAAATC AGTACCACTA CGTGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666319.2
CDS	36..2315
Translation	MADEGPRKGSVSALMGRTNGLTKPAALAGGPAKPGGTGGSRKLVIKNFRDRPRLPDNYTQDTWRKLHEAVKAIQSSTSIRYNLEELYQAVENLCSHKVSPTLYKQLRQVCEDHVQAQILPFREDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRNHIISDRMVQSKTIDGILLLIGRERSGEAVDRSLLRSLLSMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQDREVPEYLNHVSKRLEEEADRVITYLDHSTQKPLIACVEKQLLGEHLTAILQKGLEHLLDENRVPDLTQMYQLFSRVKGGQHALLQHWSEYIKTFGTTIVINPEKDKDMVQDLLDFKDKVDHVVEVCFQRNERFINLMKESFETFINKRPNKPAELIAKHVDSKLRAGNKEATDEELERILDKIMILFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHFKQHMQNQSAPGPIDLTVNILTMGYWPTYTPMEVHLPPEMVRLQEVFKTFYLGKHSGRKLQWQTTLGHAVLKADFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNADFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDSPNQYHYVA

Target ORF information:

RefSeq Version	NM_146207.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cullin 4A (Cul4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146207.2

ORF Insert Sequence:

ATGGCGGACG AGGGCCCTCG GAAGGGCAGC GTCTCGGCTC TCATGGGCCG CACCAACGGC 
CTCACCAAGC CCGCGGCGTT GGCCGGTGGC CCCGCCAAGC CGGGGGGCAC GGGAGGCTCC 
AGGAAGCTGG TCATCAAGAA CTTCCGAGAC AGGCCGCGGC TGCCTGACAA CTACACTCAG 
GACACGTGGC GGAAGCTTCA CGAAGCGGTC AAGGCCATCC AGAGCAGCAC ATCCATCAGG 
TACAACCTGG AGGAGCTGTA CCAGGCTGTT GAAAATCTTT GTTCTCACAA AGTCTCCCCA 
ACGCTCTACA AGCAGCTGCG CCAGGTCTGT GAAGACCATG TCCAGGCCCA GATCCTCCCA 
TTCAGAGAAG ACTCACTAGA CAGCGTTTTA TTTCTAAAGA AGATTAACAC ATGCTGGCAA 
GATCACTGCA GACAAATGAT CATGATCAGA AGCATCTTCC TGTTTCTGGA TCGAACCTAC 
GTCCTCCAGA ATTCCATGCT GCCTTCTATC TGGGACATGG GCCTAGAGCT GTTTAGGAAC 
CACATCATCA GCGACAGGAT GGTGCAGAGT AAGACCATTG ATGGGATCCT GCTGCTGATT 
GGGCGTGAAC GAAGTGGAGA GGCTGTGGAC AGGAGTCTGC TTCGGAGTTT GCTAAGCATG 
CTCTCAGATC TCCAGGTGTA CAAAGACTCA TTTGAGCTGA AGTTCTTGGA AGAGACTAAC 
TGTCTATACG CTGCTGAAGG CCAGAGGCTG ATGCAGGACA GGGAGGTTCC AGAATACCTT 
AACCATGTGA GCAAACGTCT AGAAGAGGAA GCAGACCGAG TGATCACCTA CTTAGACCAC 
AGCACACAAA AACCGTTGAT TGCCTGTGTG GAGAAACAGC TGTTAGGAGA ACATTTGACA 
GCAATTCTAC AGAAAGGCCT AGAGCACCTG CTGGATGAGA ACAGGGTGCC CGACCTCACC 
CAGATGTACC AGCTCTTCAG CCGGGTGAAA GGAGGGCAGC ATGCGCTGCT GCAGCACTGG 
AGCGAGTACA TCAAGACCTT TGGAACAACC ATTGTCATCA ATCCTGAGAA AGACAAAGAC 
ATGGTCCAAG ACCTGCTGGA CTTTAAGGAC AAAGTGGACC ACGTGGTGGA GGTGTGCTTC 
CAGAGGAACG AGCGCTTCAT CAACCTGATG AAGGAGTCCT TCGAGACGTT CATCAACAAG 
AGACCAAACA AGCCTGCAGA GCTCATTGCC AAGCACGTGG ACTCAAAGTT ACGGGCCGGC 
AACAAAGAAG CCACAGATGA GGAACTGGAG AGGATCCTGG ACAAGATCAT GATTCTCTTC 
CGTTTCATCC ATGGTAAAGA TGTCTTTGAA GCGTTTTATA AGAAAGACTT AGCAAAAAGG 
CTGCTGGTGG GGAAGAGCGC GTCGGTGGAC GCGGAGAAGT CCATGCTGTC GAAGCTCAAG 
CACGAGTGCG GAGCTGCGTT TACCAGCAAA CTGGAGGGCA TGTTCAAGGA CATGGAGCTC 
TCCAAAGACA TCATGGTTCA CTTCAAGCAG CATATGCAGA ACCAGAGTGC GCCAGGCCCC 
ATTGACCTCA CGGTGAACAT ACTCACCATG GGCTACTGGC CCACGTACAC ACCCATGGAA 
GTGCACCTCC CTCCAGAGAT GGTCAGACTT CAGGAAGTAT TCAAGACGTT CTACCTGGGC 
AAGCACAGTG GGCGGAAGCT GCAATGGCAG ACAACGCTGG GACACGCCGT GCTGAAGGCG 
GACTTTAAGG AGGGGAAGAA GGAGTTCCAG GTATCCCTCT TCCAGACGCT GGTGCTGCTC 
ATGTTCAACG AAGGGGATGG ATTTAGCTTT GAAGAAATAA AAATGGCCAC TGGCATAGAG 
GACAGTGAGT TGCGAAGAAC ACTACAGTCT CTGGCCTGTG GTAAAGCACG GGTCCTAATT 
AAAAGTCCCA AAGGAAAGGA GGTGGAAGAT GGAGATAAGT TCATCTTCAA TGCAGACTTC 
AAACACAAGT TGTTCAGAAT AAAGATCAAT CAAATCCAGA TGAAGGAGAC CGTTGAAGAA 
CAGGTTAGCA CTACAGAGAG AGTATTCCAG GATAGACAGT ACCAGATCGA CGCTGCTATA 
GTCAGAATAA TGAAAATGAG GAAGACACTT GGTCATAATC TTCTAGTTTC TGAATTATAC 
AATCAGCTGA AATTTCCAGT GAAGCCTGGA GATTTGAAAA AAAGGATTGA GTCACTTATA 
GACAGAGACT ATATGGAACG AGACAAAGAC AGTCCAAATC AGTACCACTA CGTGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul4a ( NM_146207.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666319.2 Mus musculus cullin 4A (Cul4a), transcript variant 1, mRNA.

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CloneID	OMu22530
Clone ID Related Accession (Same CDS sequence)	NM_146207.2 , NM_146207.3
Accession Version	NM_146207.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	cullin-4A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC133520.4. On May 18, 2018 this sequence version replaced NM_146207.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.152470.1, SRR1660817.84716.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG AGGGCCCTCG GAAGGGCAGC GTCTCGGCTC TCATGGGCCG CACCAACGGC 
CTCACCAAGC CCGCGGCGTT GGCCGGTGGC CCCGCCAAGC CGGGGGGCAC GGGAGGCTCC 
AGGAAGCTGG TCATCAAGAA CTTCCGAGAC AGGCCGCGGC TGCCTGACAA CTACACTCAG 
GACACGTGGC GGAAGCTTCA CGAAGCGGTC AAGGCCATCC AGAGCAGCAC ATCCATCAGG 
TACAACCTGG AGGAGCTGTA CCAGGCTGTT GAAAATCTTT GTTCTCACAA AGTCTCCCCA 
ACGCTCTACA AGCAGCTGCG CCAGGTCTGT GAAGACCATG TCCAGGCCCA GATCCTCCCA 
TTCAGAGAAG ACTCACTAGA CAGCGTTTTA TTTCTAAAGA AGATTAACAC ATGCTGGCAA 
GATCACTGCA GACAAATGAT CATGATCAGA AGCATCTTCC TGTTTCTGGA TCGAACCTAC 
GTCCTCCAGA ATTCCATGCT GCCTTCTATC TGGGACATGG GCCTAGAGCT GTTTAGGAAC 
CACATCATCA GCGACAGGAT GGTGCAGAGT AAGACCATTG ATGGGATCCT GCTGCTGATT 
GGGCGTGAAC GAAGTGGAGA GGCTGTGGAC AGGAGTCTGC TTCGGAGTTT GCTAAGCATG 
CTCTCAGATC TCCAGGTGTA CAAAGACTCA TTTGAGCTGA AGTTCTTGGA AGAGACTAAC 
TGTCTATACG CTGCTGAAGG CCAGAGGCTG ATGCAGGACA GGGAGGTTCC AGAATACCTT 
AACCATGTGA GCAAACGTCT AGAAGAGGAA GCAGACCGAG TGATCACCTA CTTAGACCAC 
AGCACACAAA AACCGTTGAT TGCCTGTGTG GAGAAACAGC TGTTAGGAGA ACATTTGACA 
GCAATTCTAC AGAAAGGCCT AGAGCACCTG CTGGATGAGA ACAGGGTGCC CGACCTCACC 
CAGATGTACC AGCTCTTCAG CCGGGTGAAA GGAGGGCAGC ATGCGCTGCT GCAGCACTGG 
AGCGAGTACA TCAAGACCTT TGGAACAACC ATTGTCATCA ATCCTGAGAA AGACAAAGAC 
ATGGTCCAAG ACCTGCTGGA CTTTAAGGAC AAAGTGGACC ACGTGGTGGA GGTGTGCTTC 
CAGAGGAACG AGCGCTTCAT CAACCTGATG AAGGAGTCCT TCGAGACGTT CATCAACAAG 
AGACCAAACA AGCCTGCAGA GCTCATTGCC AAGCACGTGG ACTCAAAGTT ACGGGCCGGC 
AACAAAGAAG CCACAGATGA GGAACTGGAG AGGATCCTGG ACAAGATCAT GATTCTCTTC 
CGTTTCATCC ATGGTAAAGA TGTCTTTGAA GCGTTTTATA AGAAAGACTT AGCAAAAAGG 
CTGCTGGTGG GGAAGAGCGC GTCGGTGGAC GCGGAGAAGT CCATGCTGTC GAAGCTCAAG 
CACGAGTGCG GAGCTGCGTT TACCAGCAAA CTGGAGGGCA TGTTCAAGGA CATGGAGCTC 
TCCAAAGACA TCATGGTTCA CTTCAAGCAG CATATGCAGA ACCAGAGTGC GCCAGGCCCC 
ATTGACCTCA CGGTGAACAT ACTCACCATG GGCTACTGGC CCACGTACAC ACCCATGGAA 
GTGCACCTCC CTCCAGAGAT GGTCAGACTT CAGGAAGTAT TCAAGACGTT CTACCTGGGC 
AAGCACAGTG GGCGGAAGCT GCAATGGCAG ACAACGCTGG GACACGCCGT GCTGAAGGCG 
GACTTTAAGG AGGGGAAGAA GGAGTTCCAG GTATCCCTCT TCCAGACGCT GGTGCTGCTC 
ATGTTCAACG AAGGGGATGG ATTTAGCTTT GAAGAAATAA AAATGGCCAC TGGCATAGAG 
GACAGTGAGT TGCGAAGAAC ACTACAGTCT CTGGCCTGTG GTAAAGCACG GGTCCTAATT 
AAAAGTCCCA AAGGAAAGGA GGTGGAAGAT GGAGATAAGT TCATCTTCAA TGCAGACTTC 
AAACACAAGT TGTTCAGAAT AAAGATCAAT CAAATCCAGA TGAAGGAGAC CGTTGAAGAA 
CAGGTTAGCA CTACAGAGAG AGTATTCCAG GATAGACAGT ACCAGATCGA CGCTGCTATA 
GTCAGAATAA TGAAAATGAG GAAGACACTT GGTCATAATC TTCTAGTTTC TGAATTATAC 
AATCAGCTGA AATTTCCAGT GAAGCCTGGA GATTTGAAAA AAAGGATTGA GTCACTTATA 
GACAGAGACT ATATGGAACG AGACAAAGAC AGTCCAAATC AGTACCACTA CGTGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666319.2
CDS	134..2413
Translation	MADEGPRKGSVSALMGRTNGLTKPAALAGGPAKPGGTGGSRKLVIKNFRDRPRLPDNYTQDTWRKLHEAVKAIQSSTSIRYNLEELYQAVENLCSHKVSPTLYKQLRQVCEDHVQAQILPFREDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRNHIISDRMVQSKTIDGILLLIGRERSGEAVDRSLLRSLLSMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQDREVPEYLNHVSKRLEEEADRVITYLDHSTQKPLIACVEKQLLGEHLTAILQKGLEHLLDENRVPDLTQMYQLFSRVKGGQHALLQHWSEYIKTFGTTIVINPEKDKDMVQDLLDFKDKVDHVVEVCFQRNERFINLMKESFETFINKRPNKPAELIAKHVDSKLRAGNKEATDEELERILDKIMILFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHFKQHMQNQSAPGPIDLTVNILTMGYWPTYTPMEVHLPPEMVRLQEVFKTFYLGKHSGRKLQWQTTLGHAVLKADFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNADFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDSPNQYHYVA

Target ORF information:

RefSeq Version	NM_146207.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cullin 4A (Cul4a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146207.3

ORF Insert Sequence:

ATGGCGGACG AGGGCCCTCG GAAGGGCAGC GTCTCGGCTC TCATGGGCCG CACCAACGGC 
CTCACCAAGC CCGCGGCGTT GGCCGGTGGC CCCGCCAAGC CGGGGGGCAC GGGAGGCTCC 
AGGAAGCTGG TCATCAAGAA CTTCCGAGAC AGGCCGCGGC TGCCTGACAA CTACACTCAG 
GACACGTGGC GGAAGCTTCA CGAAGCGGTC AAGGCCATCC AGAGCAGCAC ATCCATCAGG 
TACAACCTGG AGGAGCTGTA CCAGGCTGTT GAAAATCTTT GTTCTCACAA AGTCTCCCCA 
ACGCTCTACA AGCAGCTGCG CCAGGTCTGT GAAGACCATG TCCAGGCCCA GATCCTCCCA 
TTCAGAGAAG ACTCACTAGA CAGCGTTTTA TTTCTAAAGA AGATTAACAC ATGCTGGCAA 
GATCACTGCA GACAAATGAT CATGATCAGA AGCATCTTCC TGTTTCTGGA TCGAACCTAC 
GTCCTCCAGA ATTCCATGCT GCCTTCTATC TGGGACATGG GCCTAGAGCT GTTTAGGAAC 
CACATCATCA GCGACAGGAT GGTGCAGAGT AAGACCATTG ATGGGATCCT GCTGCTGATT 
GGGCGTGAAC GAAGTGGAGA GGCTGTGGAC AGGAGTCTGC TTCGGAGTTT GCTAAGCATG 
CTCTCAGATC TCCAGGTGTA CAAAGACTCA TTTGAGCTGA AGTTCTTGGA AGAGACTAAC 
TGTCTATACG CTGCTGAAGG CCAGAGGCTG ATGCAGGACA GGGAGGTTCC AGAATACCTT 
AACCATGTGA GCAAACGTCT AGAAGAGGAA GCAGACCGAG TGATCACCTA CTTAGACCAC 
AGCACACAAA AACCGTTGAT TGCCTGTGTG GAGAAACAGC TGTTAGGAGA ACATTTGACA 
GCAATTCTAC AGAAAGGCCT AGAGCACCTG CTGGATGAGA ACAGGGTGCC CGACCTCACC 
CAGATGTACC AGCTCTTCAG CCGGGTGAAA GGAGGGCAGC ATGCGCTGCT GCAGCACTGG 
AGCGAGTACA TCAAGACCTT TGGAACAACC ATTGTCATCA ATCCTGAGAA AGACAAAGAC 
ATGGTCCAAG ACCTGCTGGA CTTTAAGGAC AAAGTGGACC ACGTGGTGGA GGTGTGCTTC 
CAGAGGAACG AGCGCTTCAT CAACCTGATG AAGGAGTCCT TCGAGACGTT CATCAACAAG 
AGACCAAACA AGCCTGCAGA GCTCATTGCC AAGCACGTGG ACTCAAAGTT ACGGGCCGGC 
AACAAAGAAG CCACAGATGA GGAACTGGAG AGGATCCTGG ACAAGATCAT GATTCTCTTC 
CGTTTCATCC ATGGTAAAGA TGTCTTTGAA GCGTTTTATA AGAAAGACTT AGCAAAAAGG 
CTGCTGGTGG GGAAGAGCGC GTCGGTGGAC GCGGAGAAGT CCATGCTGTC GAAGCTCAAG 
CACGAGTGCG GAGCTGCGTT TACCAGCAAA CTGGAGGGCA TGTTCAAGGA CATGGAGCTC 
TCCAAAGACA TCATGGTTCA CTTCAAGCAG CATATGCAGA ACCAGAGTGC GCCAGGCCCC 
ATTGACCTCA CGGTGAACAT ACTCACCATG GGCTACTGGC CCACGTACAC ACCCATGGAA 
GTGCACCTCC CTCCAGAGAT GGTCAGACTT CAGGAAGTAT TCAAGACGTT CTACCTGGGC 
AAGCACAGTG GGCGGAAGCT GCAATGGCAG ACAACGCTGG GACACGCCGT GCTGAAGGCG 
GACTTTAAGG AGGGGAAGAA GGAGTTCCAG GTATCCCTCT TCCAGACGCT GGTGCTGCTC 
ATGTTCAACG AAGGGGATGG ATTTAGCTTT GAAGAAATAA AAATGGCCAC TGGCATAGAG 
GACAGTGAGT TGCGAAGAAC ACTACAGTCT CTGGCCTGTG GTAAAGCACG GGTCCTAATT 
AAAAGTCCCA AAGGAAAGGA GGTGGAAGAT GGAGATAAGT TCATCTTCAA TGCAGACTTC 
AAACACAAGT TGTTCAGAAT AAAGATCAAT CAAATCCAGA TGAAGGAGAC CGTTGAAGAA 
CAGGTTAGCA CTACAGAGAG AGTATTCCAG GATAGACAGT ACCAGATCGA CGCTGCTATA 
GTCAGAATAA TGAAAATGAG GAAGACACTT GGTCATAATC TTCTAGTTTC TGAATTATAC 
AATCAGCTGA AATTTCCAGT GAAGCCTGGA GATTTGAAAA AAAGGATTGA GTCACTTATA 
GACAGAGACT ATATGGAACG AGACAAAGAC AGTCCAAATC AGTACCACTA CGTGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul4a ( NM_001363448.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001350377.1 Mus musculus cullin 4A (Cul4a), transcript variant 2, mRNA.

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CloneID	OMu40503
Clone ID Related Accession (Same CDS sequence)	XM_006508933.1 , NM_001363448.1
Accession Version	NM_001363448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	cullin-4A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC133520.4. On May 18, 2018 this sequence version replaced XM_006508933.1. Transcript Variant: This variant (2) lacks an alternate in-frame segment of two consecutive exons compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG AGGGCCCTCG GAAGGGCAGC GTCTCGGCTC TCATGGGCCG CACCAACGGC 
CTCACCAAGC CCGCGGCGTT GGCCGGTGGC CCCGCCAAGC CGGGGGGCAC GGGAGGCTCC 
AGGAAGCTGG TCATCAAGAA CTTCCGAGAC AGGCCGCGGC TGCCTGACAA CTACACTCAG 
GACACGTGGC GGAAGCTTCA CGAAGCGGTC AAGGCCATCC AGAGCAGCAC ATCCATCAGG 
TACAACCTGG AGGAGCTGTA CCAGATCATG ATCAGAAGCA TCTTCCTGTT TCTGGATCGA 
ACCTACGTCC TCCAGAATTC CATGCTGCCT TCTATCTGGG ACATGGGCCT AGAGCTGTTT 
AGGAACCACA TCATCAGCGA CAGGATGGTG CAGAGTAAGA CCATTGATGG GATCCTGCTG 
CTGATTGGGC GTGAACGAAG TGGAGAGGCT GTGGACAGGA GTCTGCTTCG GAGTTTGCTA 
AGCATGCTCT CAGATCTCCA GGTGTACAAA GACTCATTTG AGCTGAAGTT CTTGGAAGAG 
ACTAACTGTC TATACGCTGC TGAAGGCCAG AGGCTGATGC AGGACAGGGA GGTTCCAGAA 
TACCTTAACC ATGTGAGCAA ACGTCTAGAA GAGGAAGCAG ACCGAGTGAT CACCTACTTA 
GACCACAGCA CACAAAAACC GTTGATTGCC TGTGTGGAGA AACAGCTGTT AGGAGAACAT 
TTGACAGCAA TTCTACAGAA AGGCCTAGAG CACCTGCTGG ATGAGAACAG GGTGCCCGAC 
CTCACCCAGA TGTACCAGCT CTTCAGCCGG GTGAAAGGAG GGCAGCATGC GCTGCTGCAG 
CACTGGAGCG AGTACATCAA GACCTTTGGA ACAACCATTG TCATCAATCC TGAGAAAGAC 
AAAGACATGG TCCAAGACCT GCTGGACTTT AAGGACAAAG TGGACCACGT GGTGGAGGTG 
TGCTTCCAGA GGAACGAGCG CTTCATCAAC CTGATGAAGG AGTCCTTCGA GACGTTCATC 
AACAAGAGAC CAAACAAGCC TGCAGAGCTC ATTGCCAAGC ACGTGGACTC AAAGTTACGG 
GCCGGCAACA AAGAAGCCAC AGATGAGGAA CTGGAGAGGA TCCTGGACAA GATCATGATT 
CTCTTCCGTT TCATCCATGG TAAAGATGTC TTTGAAGCGT TTTATAAGAA AGACTTAGCA 
AAAAGGCTGC TGGTGGGGAA GAGCGCGTCG GTGGACGCGG AGAAGTCCAT GCTGTCGAAG 
CTCAAGCACG AGTGCGGAGC TGCGTTTACC AGCAAACTGG AGGGCATGTT CAAGGACATG 
GAGCTCTCCA AAGACATCAT GGTTCACTTC AAGCAGCATA TGCAGAACCA GAGTGCGCCA 
GGCCCCATTG ACCTCACGGT GAACATACTC ACCATGGGCT ACTGGCCCAC GTACACACCC 
ATGGAAGTGC ACCTCCCTCC AGAGATGGTC AGACTTCAGG AAGTATTCAA GACGTTCTAC 
CTGGGCAAGC ACAGTGGGCG GAAGCTGCAA TGGCAGACAA CGCTGGGACA CGCCGTGCTG 
AAGGCGGACT TTAAGGAGGG GAAGAAGGAG TTCCAGGTAT CCCTCTTCCA GACGCTGGTG 
CTGCTCATGT TCAACGAAGG GGATGGATTT AGCTTTGAAG AAATAAAAAT GGCCACTGGC 
ATAGAGGACA GTGAGTTGCG AAGAACACTA CAGTCTCTGG CCTGTGGTAA AGCACGGGTC 
CTAATTAAAA GTCCCAAAGG AAAGGAGGTG GAAGATGGAG ATAAGTTCAT CTTCAATGCA 
GACTTCAAAC ACAAGTTGTT CAGAATAAAG ATCAATCAAA TCCAGATGAA GGAGACCGTT 
GAAGAACAGG TTAGCACTAC AGAGAGAGTA TTCCAGGATA GACAGTACCA GATCGACGCT 
GCTATAGTCA GAATAATGAA AATGAGGAAG ACACTTGGTC ATAATCTTCT AGTTTCTGAA 
TTATACAATC AGCTGAAATT TCCAGTGAAG CCTGGAGATT TGAAAAAAAG GATTGAGTCA 
CTTATAGACA GAGACTATAT GGAACGAGAC AAAGACAGTC CAAATCAGTA CCACTACGTG 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350377.1
CDS	134..2239
Translation	MADEGPRKGSVSALMGRTNGLTKPAALAGGPAKPGGTGGSRKLVIKNFRDRPRLPDNYTQDTWRKLHEAVKAIQSSTSIRYNLEELYQIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRNHIISDRMVQSKTIDGILLLIGRERSGEAVDRSLLRSLLSMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQDREVPEYLNHVSKRLEEEADRVITYLDHSTQKPLIACVEKQLLGEHLTAILQKGLEHLLDENRVPDLTQMYQLFSRVKGGQHALLQHWSEYIKTFGTTIVINPEKDKDMVQDLLDFKDKVDHVVEVCFQRNERFINLMKESFETFINKRPNKPAELIAKHVDSKLRAGNKEATDEELERILDKIMILFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHFKQHMQNQSAPGPIDLTVNILTMGYWPTYTPMEVHLPPEMVRLQEVFKTFYLGKHSGRKLQWQTTLGHAVLKADFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNADFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDSPNQYHYVA

Target ORF information:

RefSeq Version	NM_001363448.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cullin 4A (Cul4a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363448.1

ORF Insert Sequence:

ATGGCGGACG AGGGCCCTCG GAAGGGCAGC GTCTCGGCTC TCATGGGCCG CACCAACGGC 
CTCACCAAGC CCGCGGCGTT GGCCGGTGGC CCCGCCAAGC CGGGGGGCAC GGGAGGCTCC 
AGGAAGCTGG TCATCAAGAA CTTCCGAGAC AGGCCGCGGC TGCCTGACAA CTACACTCAG 
GACACGTGGC GGAAGCTTCA CGAAGCGGTC AAGGCCATCC AGAGCAGCAC ATCCATCAGG 
TACAACCTGG AGGAGCTGTA CCAGATCATG ATCAGAAGCA TCTTCCTGTT TCTGGATCGA 
ACCTACGTCC TCCAGAATTC CATGCTGCCT TCTATCTGGG ACATGGGCCT AGAGCTGTTT 
AGGAACCACA TCATCAGCGA CAGGATGGTG CAGAGTAAGA CCATTGATGG GATCCTGCTG 
CTGATTGGGC GTGAACGAAG TGGAGAGGCT GTGGACAGGA GTCTGCTTCG GAGTTTGCTA 
AGCATGCTCT CAGATCTCCA GGTGTACAAA GACTCATTTG AGCTGAAGTT CTTGGAAGAG 
ACTAACTGTC TATACGCTGC TGAAGGCCAG AGGCTGATGC AGGACAGGGA GGTTCCAGAA 
TACCTTAACC ATGTGAGCAA ACGTCTAGAA GAGGAAGCAG ACCGAGTGAT CACCTACTTA 
GACCACAGCA CACAAAAACC GTTGATTGCC TGTGTGGAGA AACAGCTGTT AGGAGAACAT 
TTGACAGCAA TTCTACAGAA AGGCCTAGAG CACCTGCTGG ATGAGAACAG GGTGCCCGAC 
CTCACCCAGA TGTACCAGCT CTTCAGCCGG GTGAAAGGAG GGCAGCATGC GCTGCTGCAG 
CACTGGAGCG AGTACATCAA GACCTTTGGA ACAACCATTG TCATCAATCC TGAGAAAGAC 
AAAGACATGG TCCAAGACCT GCTGGACTTT AAGGACAAAG TGGACCACGT GGTGGAGGTG 
TGCTTCCAGA GGAACGAGCG CTTCATCAAC CTGATGAAGG AGTCCTTCGA GACGTTCATC 
AACAAGAGAC CAAACAAGCC TGCAGAGCTC ATTGCCAAGC ACGTGGACTC AAAGTTACGG 
GCCGGCAACA AAGAAGCCAC AGATGAGGAA CTGGAGAGGA TCCTGGACAA GATCATGATT 
CTCTTCCGTT TCATCCATGG TAAAGATGTC TTTGAAGCGT TTTATAAGAA AGACTTAGCA 
AAAAGGCTGC TGGTGGGGAA GAGCGCGTCG GTGGACGCGG AGAAGTCCAT GCTGTCGAAG 
CTCAAGCACG AGTGCGGAGC TGCGTTTACC AGCAAACTGG AGGGCATGTT CAAGGACATG 
GAGCTCTCCA AAGACATCAT GGTTCACTTC AAGCAGCATA TGCAGAACCA GAGTGCGCCA 
GGCCCCATTG ACCTCACGGT GAACATACTC ACCATGGGCT ACTGGCCCAC GTACACACCC 
ATGGAAGTGC ACCTCCCTCC AGAGATGGTC AGACTTCAGG AAGTATTCAA GACGTTCTAC 
CTGGGCAAGC ACAGTGGGCG GAAGCTGCAA TGGCAGACAA CGCTGGGACA CGCCGTGCTG 
AAGGCGGACT TTAAGGAGGG GAAGAAGGAG TTCCAGGTAT CCCTCTTCCA GACGCTGGTG 
CTGCTCATGT TCAACGAAGG GGATGGATTT AGCTTTGAAG AAATAAAAAT GGCCACTGGC 
ATAGAGGACA GTGAGTTGCG AAGAACACTA CAGTCTCTGG CCTGTGGTAA AGCACGGGTC 
CTAATTAAAA GTCCCAAAGG AAAGGAGGTG GAAGATGGAG ATAAGTTCAT CTTCAATGCA 
GACTTCAAAC ACAAGTTGTT CAGAATAAAG ATCAATCAAA TCCAGATGAA GGAGACCGTT 
GAAGAACAGG TTAGCACTAC AGAGAGAGTA TTCCAGGATA GACAGTACCA GATCGACGCT 
GCTATAGTCA GAATAATGAA AATGAGGAAG ACACTTGGTC ATAATCTTCT AGTTTCTGAA 
TTATACAATC AGCTGAAATT TCCAGTGAAG CCTGGAGATT TGAAAAAAAG GATTGAGTCA 
CTTATAGACA GAGACTATAT GGAACGAGAC AAAGACAGTC CAAATCAGTA CCACTACGTG 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul4a ( XM_006508933.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508996.1 Mus musculus cullin 4A (Cul4a), transcript variant X1, mRNA.

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CloneID	OMu40503
Clone ID Related Accession (Same CDS sequence)	XM_006508933.1 , NM_001363448.1
Accession Version	XM_006508933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cullin-4A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG AGGGCCCTCG GAAGGGCAGC GTCTCGGCTC TCATGGGCCG CACCAACGGC 
CTCACCAAGC CCGCGGCGTT GGCCGGTGGC CCCGCCAAGC CGGGGGGCAC GGGAGGCTCC 
AGGAAGCTGG TCATCAAGAA CTTCCGAGAC AGGCCGCGGC TGCCTGACAA CTACACTCAG 
GACACGTGGC GGAAGCTTCA CGAAGCGGTC AAGGCCATCC AGAGCAGCAC ATCCATCAGG 
TACAACCTGG AGGAGCTGTA CCAGATCATG ATCAGAAGCA TCTTCCTGTT TCTGGATCGA 
ACCTACGTCC TCCAGAATTC CATGCTGCCT TCTATCTGGG ACATGGGCCT AGAGCTGTTT 
AGGAACCACA TCATCAGCGA CAGGATGGTG CAGAGTAAGA CCATTGATGG GATCCTGCTG 
CTGATTGGGC GTGAACGAAG TGGAGAGGCT GTGGACAGGA GTCTGCTTCG GAGTTTGCTA 
AGCATGCTCT CAGATCTCCA GGTGTACAAA GACTCATTTG AGCTGAAGTT CTTGGAAGAG 
ACTAACTGTC TATACGCTGC TGAAGGCCAG AGGCTGATGC AGGACAGGGA GGTTCCAGAA 
TACCTTAACC ATGTGAGCAA ACGTCTAGAA GAGGAAGCAG ACCGAGTGAT CACCTACTTA 
GACCACAGCA CACAAAAACC GTTGATTGCC TGTGTGGAGA AACAGCTGTT AGGAGAACAT 
TTGACAGCAA TTCTACAGAA AGGCCTAGAG CACCTGCTGG ATGAGAACAG GGTGCCCGAC 
CTCACCCAGA TGTACCAGCT CTTCAGCCGG GTGAAAGGAG GGCAGCATGC GCTGCTGCAG 
CACTGGAGCG AGTACATCAA GACCTTTGGA ACAACCATTG TCATCAATCC TGAGAAAGAC 
AAAGACATGG TCCAAGACCT GCTGGACTTT AAGGACAAAG TGGACCACGT GGTGGAGGTG 
TGCTTCCAGA GGAACGAGCG CTTCATCAAC CTGATGAAGG AGTCCTTCGA GACGTTCATC 
AACAAGAGAC CAAACAAGCC TGCAGAGCTC ATTGCCAAGC ACGTGGACTC AAAGTTACGG 
GCCGGCAACA AAGAAGCCAC AGATGAGGAA CTGGAGAGGA TCCTGGACAA GATCATGATT 
CTCTTCCGTT TCATCCATGG TAAAGATGTC TTTGAAGCGT TTTATAAGAA AGACTTAGCA 
AAAAGGCTGC TGGTGGGGAA GAGCGCGTCG GTGGACGCGG AGAAGTCCAT GCTGTCGAAG 
CTCAAGCACG AGTGCGGAGC TGCGTTTACC AGCAAACTGG AGGGCATGTT CAAGGACATG 
GAGCTCTCCA AAGACATCAT GGTTCACTTC AAGCAGCATA TGCAGAACCA GAGTGCGCCA 
GGCCCCATTG ACCTCACGGT GAACATACTC ACCATGGGCT ACTGGCCCAC GTACACACCC 
ATGGAAGTGC ACCTCCCTCC AGAGATGGTC AGACTTCAGG AAGTATTCAA GACGTTCTAC 
CTGGGCAAGC ACAGTGGGCG GAAGCTGCAA TGGCAGACAA CGCTGGGACA CGCCGTGCTG 
AAGGCGGACT TTAAGGAGGG GAAGAAGGAG TTCCAGGTAT CCCTCTTCCA GACGCTGGTG 
CTGCTCATGT TCAACGAAGG GGATGGATTT AGCTTTGAAG AAATAAAAAT GGCCACTGGC 
ATAGAGGACA GTGAGTTGCG AAGAACACTA CAGTCTCTGG CCTGTGGTAA AGCACGGGTC 
CTAATTAAAA GTCCCAAAGG AAAGGAGGTG GAAGATGGAG ATAAGTTCAT CTTCAATGCA 
GACTTCAAAC ACAAGTTGTT CAGAATAAAG ATCAATCAAA TCCAGATGAA GGAGACCGTT 
GAAGAACAGG TTAGCACTAC AGAGAGAGTA TTCCAGGATA GACAGTACCA GATCGACGCT 
GCTATAGTCA GAATAATGAA AATGAGGAAG ACACTTGGTC ATAATCTTCT AGTTTCTGAA 
TTATACAATC AGCTGAAATT TCCAGTGAAG CCTGGAGATT TGAAAAAAAG GATTGAGTCA 
CTTATAGACA GAGACTATAT GGAACGAGAC AAAGACAGTC CAAATCAGTA CCACTACGTG 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508996.1
CDS	68..2173
Translation	MADEGPRKGSVSALMGRTNGLTKPAALAGGPAKPGGTGGSRKLVIKNFRDRPRLPDNYTQDTWRKLHEAVKAIQSSTSIRYNLEELYQIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRNHIISDRMVQSKTIDGILLLIGRERSGEAVDRSLLRSLLSMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQDREVPEYLNHVSKRLEEEADRVITYLDHSTQKPLIACVEKQLLGEHLTAILQKGLEHLLDENRVPDLTQMYQLFSRVKGGQHALLQHWSEYIKTFGTTIVINPEKDKDMVQDLLDFKDKVDHVVEVCFQRNERFINLMKESFETFINKRPNKPAELIAKHVDSKLRAGNKEATDEELERILDKIMILFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHFKQHMQNQSAPGPIDLTVNILTMGYWPTYTPMEVHLPPEMVRLQEVFKTFYLGKHSGRKLQWQTTLGHAVLKADFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNADFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDSPNQYHYVA

Target ORF information:

RefSeq Version	XM_006508933.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cullin 4A (Cul4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508933.1

ORF Insert Sequence:

ATGGCGGACG AGGGCCCTCG GAAGGGCAGC GTCTCGGCTC TCATGGGCCG CACCAACGGC 
CTCACCAAGC CCGCGGCGTT GGCCGGTGGC CCCGCCAAGC CGGGGGGCAC GGGAGGCTCC 
AGGAAGCTGG TCATCAAGAA CTTCCGAGAC AGGCCGCGGC TGCCTGACAA CTACACTCAG 
GACACGTGGC GGAAGCTTCA CGAAGCGGTC AAGGCCATCC AGAGCAGCAC ATCCATCAGG 
TACAACCTGG AGGAGCTGTA CCAGATCATG ATCAGAAGCA TCTTCCTGTT TCTGGATCGA 
ACCTACGTCC TCCAGAATTC CATGCTGCCT TCTATCTGGG ACATGGGCCT AGAGCTGTTT 
AGGAACCACA TCATCAGCGA CAGGATGGTG CAGAGTAAGA CCATTGATGG GATCCTGCTG 
CTGATTGGGC GTGAACGAAG TGGAGAGGCT GTGGACAGGA GTCTGCTTCG GAGTTTGCTA 
AGCATGCTCT CAGATCTCCA GGTGTACAAA GACTCATTTG AGCTGAAGTT CTTGGAAGAG 
ACTAACTGTC TATACGCTGC TGAAGGCCAG AGGCTGATGC AGGACAGGGA GGTTCCAGAA 
TACCTTAACC ATGTGAGCAA ACGTCTAGAA GAGGAAGCAG ACCGAGTGAT CACCTACTTA 
GACCACAGCA CACAAAAACC GTTGATTGCC TGTGTGGAGA AACAGCTGTT AGGAGAACAT 
TTGACAGCAA TTCTACAGAA AGGCCTAGAG CACCTGCTGG ATGAGAACAG GGTGCCCGAC 
CTCACCCAGA TGTACCAGCT CTTCAGCCGG GTGAAAGGAG GGCAGCATGC GCTGCTGCAG 
CACTGGAGCG AGTACATCAA GACCTTTGGA ACAACCATTG TCATCAATCC TGAGAAAGAC 
AAAGACATGG TCCAAGACCT GCTGGACTTT AAGGACAAAG TGGACCACGT GGTGGAGGTG 
TGCTTCCAGA GGAACGAGCG CTTCATCAAC CTGATGAAGG AGTCCTTCGA GACGTTCATC 
AACAAGAGAC CAAACAAGCC TGCAGAGCTC ATTGCCAAGC ACGTGGACTC AAAGTTACGG 
GCCGGCAACA AAGAAGCCAC AGATGAGGAA CTGGAGAGGA TCCTGGACAA GATCATGATT 
CTCTTCCGTT TCATCCATGG TAAAGATGTC TTTGAAGCGT TTTATAAGAA AGACTTAGCA 
AAAAGGCTGC TGGTGGGGAA GAGCGCGTCG GTGGACGCGG AGAAGTCCAT GCTGTCGAAG 
CTCAAGCACG AGTGCGGAGC TGCGTTTACC AGCAAACTGG AGGGCATGTT CAAGGACATG 
GAGCTCTCCA AAGACATCAT GGTTCACTTC AAGCAGCATA TGCAGAACCA GAGTGCGCCA 
GGCCCCATTG ACCTCACGGT GAACATACTC ACCATGGGCT ACTGGCCCAC GTACACACCC 
ATGGAAGTGC ACCTCCCTCC AGAGATGGTC AGACTTCAGG AAGTATTCAA GACGTTCTAC 
CTGGGCAAGC ACAGTGGGCG GAAGCTGCAA TGGCAGACAA CGCTGGGACA CGCCGTGCTG 
AAGGCGGACT TTAAGGAGGG GAAGAAGGAG TTCCAGGTAT CCCTCTTCCA GACGCTGGTG 
CTGCTCATGT TCAACGAAGG GGATGGATTT AGCTTTGAAG AAATAAAAAT GGCCACTGGC 
ATAGAGGACA GTGAGTTGCG AAGAACACTA CAGTCTCTGG CCTGTGGTAA AGCACGGGTC 
CTAATTAAAA GTCCCAAAGG AAAGGAGGTG GAAGATGGAG ATAAGTTCAT CTTCAATGCA 
GACTTCAAAC ACAAGTTGTT CAGAATAAAG ATCAATCAAA TCCAGATGAA GGAGACCGTT 
GAAGAACAGG TTAGCACTAC AGAGAGAGTA TTCCAGGATA GACAGTACCA GATCGACGCT 
GCTATAGTCA GAATAATGAA AATGAGGAAG ACACTTGGTC ATAATCTTCT AGTTTCTGAA 
TTATACAATC AGCTGAAATT TCCAGTGAAG CCTGGAGATT TGAAAAAAAG GATTGAGTCA 
CTTATAGACA GAGACTATAT GGAACGAGAC AAAGACAGTC CAAATCAGTA CCACTACGTG 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CRL4
Cell reports18(6)1499-1511(2017 Feb)
Li G,Ji T,Chen J,Fu Y,Hou L,Feng Y,Zhang T,Song T,Zhao J,Endo Y,Lin H,Cai X,Cang Y

Genome-wide RNAi Screening Identifies Protein Modules Required for 40S Subunit Synthesis in Human Cells.
Cell reports13(12)2879-91(2015 Dec)
Badertscher L,Wild T,Montellese C,Alexander LT,Bammert L,Sarazova M,Stebler M,Csucs G,Mayer TU,Zamboni N,Zemp I,Horvath P,Kutay U

CUL4A facilitates hepatocarcinogenesis by promoting cell cycle progression and epithelial-mesenchymal transition.
Scientific reports517006(2015 Nov)
Pan Y,Wang B,Yang X,Bai F,Xu Q,Li X,Gao L,Ma C,Liang X

The CUL4-DDB1 ubiquitin ligase complex controls adult and embryonic stem cell differentiation and homeostasis.
eLife4(2015 Nov)
Gao J,Buckley SM,Cimmino L,Guillamot M,Strikoudis A,Cang Y,Goff SP,Aifantis I

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Cul4a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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