Dcaf8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dcaf8
Entrez Gene ID	98193
Full Name	DDB1 and CUL4 associated factor 8
Synonyms	AA408877,AA433120,AA792214,AU018973,C76055,D1Dau35e,D1Ucla4,H326,Wdr42a
General protein information	Preferred Names DDB1 and CUL4 associated factor 8 Names DDB1- and CUL4-associated factor 8 WD repeat domain 42A WD repeat-containing protein 42A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 H3\|1 79.54 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006497065.3	XP_006497128.1	DDB1- and CUL4-associated factor 8 isoform X1
XM_011238889.2	XP_011237191.1	DDB1- and CUL4-associated factor 8 isoform X1
NM_153555.2	NP_705783.1	DDB1- and CUL4-associated factor 8
XM_011238889.3	XP_011237191.1	DDB1- and CUL4-associated factor 8 isoform X1
NM_001357204.1	NP_001344133.1	DDB1- and CUL4-associated factor 8

Pathways from BioSystems

Homologies

	LOC719645	XP_002808293.1
Xenopus tropicalis (tropical clawed frog)	dcaf8	NP_001016610.1
Homo sapiens (human)	DCAF8	NP_056541.2
Canis lupus familiaris (dog)	DCAF8	XP_536129.2
Bos taurus (cattle)	DCAF8	NP_001193348.1
Rattus norvegicus (Norway rat)	Dcaf8	NP_001014253.1
Gallus gallus (chicken)	DCAF8	XP_426809.4
Arabidopsis thaliana (thale cress)	AT3G45620	NP_001190014.1
Pan troglodytes (chimpanzee)	DCAF8	XP_003308591.1
Oryza sativa (rice)	Os03g0286800	NP_001049775.1
Mus musculus (house mouse)	Dcaf8	NP_705783.1
Danio rerio (zebrafish)	LOC100007924	XP_001346262.2

Gene Ontology
Bibliography

Related articles in PubMed

CRL4
Li G, Ji T, Chen J, Fu Y, Hou L, Feng Y, Zhang T, Song T, Zhao J, Endo Y, Lin H, Cai X, Cang Y
Cell reports18(6)1499-1511(2017 Feb)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Severe neural tube defects in the loop-tail mouse result from mutation of Lpp1, a novel gene involved in floor plate specification.
Murdoch JN, Doudney K, Paternotte C, Copp AJ, Stanier P
Human molecular genetics10(22)2593-601(2001 Oct)

Ltap, a mammalian homolog of Drosophila Strabismus/Van Gogh, is altered in the mouse neural tube mutant Loop-tail.
Kibar Z, Vogan KJ, Groulx N, Justice MJ, Underhill DA, Gros P
Nature genetics28(3)251-5(2001 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dcaf8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dcaf8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02851	NM_001357204.1 Latest version!	Mus musculus DDB1 and CUL4 associated factor 8 (Dcaf8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu02851	NM_153555.2 Latest version!	Mus musculus DDB1 and CUL4 associated factor 8 (Dcaf8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu02851	XM_006497065.3 Latest version!	Mus musculus DDB1 and CUL4 associated factor 8 (Dcaf8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu02851	XM_011238889.3 Latest version!	Mus musculus DDB1 and CUL4 associated factor 8 (Dcaf8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu02851	XM_011238889.2	Mus musculus DDB1 and CUL4 associated factor 8 (Dcaf8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dcaf8 ( NM_153555.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_705783.1 Mus musculus DDB1 and CUL4 associated factor 8 (Dcaf8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02851
Clone ID Related Accession (Same CDS sequence)	XM_006497065.3 , XM_011238889.2 , NM_153555.2 , XM_011238889.3 , NM_001357204.1
Accession Version	NM_153555.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	DDB1- and CUL4-associated factor 8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB851975.1, AK146825.1, AC074310.29 and AW047778.1. On Jan 10, 2008 this sequence version replaced NM_153555.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK146825.1, AK035701.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAATA AACGTCCCAA CACGACAGAT GGGAGAACAG ATTTAGCTAA TGGAAGTCTG 
TCCAGCAGCC CAGAGGAGAT GTCTGGAGCT GAAGAGGGGA GGGAAACATC CTCAGGGATT 
GAAGTAGAGG CATCAGACCT GAGTCTCAGC TTGACTGGGG ATGACGGTGG CCCCAACCGC 
ACCAGCACGG AAAGTCGTGG CACAGACACA GAGAGCTCAG GGGAAGAAAA GGACTCTGAC 
AGCATGGAGG ACACGGGCCA TTATTCCATC AATGATGAAA GCCGAGGTCA TGGCCACTCA 
GACGAAGAGG ACGAGGAACA ACCTCGGCAC CGTGGACAGC GCAAACGGGC CAGCCGTGAC 
CAGGATTCAT CAGATGATGA GCGAGCCCTG GAAGACTGGG TATCTTCAGA GACAACAGCC 
TTACCCCGAC CTCGCTGGCA AGCCCTTCCT GCGCTTCGGG AGCGGGAACT GGGTTCCAGT 
GCCCGCTTTG TGTACGAGGC TTGTGGAGCT AGAGTCTTTG TACAGCGTTT CCGTTTGCAG 
CACGGGCTTG AGGGCCATAC TGGTTGTGTC AATACCTTGC ACTTTAACCA GCGTGGTACC 
TGGCTGGCCA GTGGCAGTGA TGACCTGAAG GTGGTGGTGT GGGATTGGGT GCGGCGGCAG 
CCAGTACTGG ACTTTGAGAG TGGCCACAAA AGTAATGTCT TCCAGGCCAA GTTCCTTCCT 
AACAGTGGTG ACTCCACCCT GGCTATGTGT GCCCGGGATG GACAGGTCCG AGTCGCAGAG 
CTGTCTGCTA CACAGTGCTG CAAGAACACC AAGCGTGTGG CCCAGCACAA GGGGGCATCT 
CACAAGTTAG CCCTAGAACC AGACTCCCCC TGCACGTTCC TGTCAGCTGG TGAAGATGCC 
GTCGTCTTCA CCATTGACCT GAGACAAGAC CGGCCAGCTT CGAAACTAGT GGTGACAAAA 
GAGAAGGAGA AGAAAGTGGG GCTGTATACA ATCTATGTGA ACCCTGCCAA TACCCACCAG 
TTTGCAGTGG GTGGAAGAGA TCAGTATGTA AGGATTTATG ACCAGAGGAA AATTGATGAG 
AATGAGAACA ACGGAGTCCT GAAAAAATTC TGTCCTCACC ACTTGGTGAA CAGTGAGTCC 
AAAGCAAACA TCACCTGTCT TGTGTACAGC CACGACGGCA CAGAGCTCCT GGCCAGTTAC 
AATGATGAAG ACATTTACCT TTTCAACTCT TCTCACAGTG ATGGGGCCCA GTATATTAAG 
AGATACAAAG GCCATAGAAA TAATGCTACA GTAAAAGGCG TCAATTTCTA TGGCCCCAAG 
AGTGAGTTTG TGGTGAGCGG TAGTGACTGC GGGCACATCT TCCTCTGGGA GAAGTCATCT 
TGCCAGATTA TTCAGTTCAT GGAAGGGGAC AAGGGAGGTG TGGTAAACTG TCTTGAGCCC 
CATCCTCATC TGCCTGTTTT GGCAACCAGT GGCCTAGACC ATGATGTGAA GATCTGGGCG 
CCGACAGCTG AAGCTTCCAC TGAGCTGACA GGGTTAAAGG AAGTGATCAA GAAGAACAAG 
CGAGAGCGGG ATGAAGACAG CTTGCACCAC ACTGATCTGT TTGATAGTCA CATGCTGTGG 
TTCCTTATGC ATCACCTGAG ACAGAGACGC CATCATCGGC GCTGGCGAGA ACCTGGGGTT 
GGGGCCACAG ATGCAGACTC TGATGAGTCT CCCAGCTCCT CAGACACATC CGACGAGGAG 
GAGGGCCCTG ACCGGGTGCA GTGCATGCCA TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705783.1
CDS	247..2022
Translation	MSNKRPNTTDGRTDLANGSLSSSPEEMSGAEEGRETSSGIEVEASDLSLSLTGDDGGPNRTSTESRGTDTESSGEEKDSDSMEDTGHYSINDESRGHGHSDEEDEEQPRHRGQRKRASRDQDSSDDERALEDWVSSETTALPRPRWQALPALRERELGSSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKVVVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSATQCCKNTKRVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVGLYTIYVNPANTHQFAVGGRDQYVRIYDQRKIDENENNGVLKKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYIKRYKGHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKEVIKKNKRERDEDSLHHTDLFDSHMLWFLMHHLRQRRHHRRWREPGVGATDADSDESPSSSDTSDEEEGPDRVQCMPS

Target ORF information:

RefSeq Version	NM_153555.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DDB1 and CUL4 associated factor 8 (Dcaf8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153555.2

ORF Insert Sequence:

ATGTCCAATA AACGTCCCAA CACGACAGAT GGGAGAACAG ATTTAGCTAA TGGAAGTCTG 
TCCAGCAGCC CAGAGGAGAT GTCTGGAGCT GAAGAGGGGA GGGAAACATC CTCAGGGATT 
GAAGTAGAGG CATCAGACCT GAGTCTCAGC TTGACTGGGG ATGACGGTGG CCCCAACCGC 
ACCAGCACGG AAAGTCGTGG CACAGACACA GAGAGCTCAG GGGAAGAAAA GGACTCTGAC 
AGCATGGAGG ACACGGGCCA TTATTCCATC AATGATGAAA GCCGAGGTCA TGGCCACTCA 
GACGAAGAGG ACGAGGAACA ACCTCGGCAC CGTGGACAGC GCAAACGGGC CAGCCGTGAC 
CAGGATTCAT CAGATGATGA GCGAGCCCTG GAAGACTGGG TATCTTCAGA GACAACAGCC 
TTACCCCGAC CTCGCTGGCA AGCCCTTCCT GCGCTTCGGG AGCGGGAACT GGGTTCCAGT 
GCCCGCTTTG TGTACGAGGC TTGTGGAGCT AGAGTCTTTG TACAGCGTTT CCGTTTGCAG 
CACGGGCTTG AGGGCCATAC TGGTTGTGTC AATACCTTGC ACTTTAACCA GCGTGGTACC 
TGGCTGGCCA GTGGCAGTGA TGACCTGAAG GTGGTGGTGT GGGATTGGGT GCGGCGGCAG 
CCAGTACTGG ACTTTGAGAG TGGCCACAAA AGTAATGTCT TCCAGGCCAA GTTCCTTCCT 
AACAGTGGTG ACTCCACCCT GGCTATGTGT GCCCGGGATG GACAGGTCCG AGTCGCAGAG 
CTGTCTGCTA CACAGTGCTG CAAGAACACC AAGCGTGTGG CCCAGCACAA GGGGGCATCT 
CACAAGTTAG CCCTAGAACC AGACTCCCCC TGCACGTTCC TGTCAGCTGG TGAAGATGCC 
GTCGTCTTCA CCATTGACCT GAGACAAGAC CGGCCAGCTT CGAAACTAGT GGTGACAAAA 
GAGAAGGAGA AGAAAGTGGG GCTGTATACA ATCTATGTGA ACCCTGCCAA TACCCACCAG 
TTTGCAGTGG GTGGAAGAGA TCAGTATGTA AGGATTTATG ACCAGAGGAA AATTGATGAG 
AATGAGAACA ACGGAGTCCT GAAAAAATTC TGTCCTCACC ACTTGGTGAA CAGTGAGTCC 
AAAGCAAACA TCACCTGTCT TGTGTACAGC CACGACGGCA CAGAGCTCCT GGCCAGTTAC 
AATGATGAAG ACATTTACCT TTTCAACTCT TCTCACAGTG ATGGGGCCCA GTATATTAAG 
AGATACAAAG GCCATAGAAA TAATGCTACA GTAAAAGGCG TCAATTTCTA TGGCCCCAAG 
AGTGAGTTTG TGGTGAGCGG TAGTGACTGC GGGCACATCT TCCTCTGGGA GAAGTCATCT 
TGCCAGATTA TTCAGTTCAT GGAAGGGGAC AAGGGAGGTG TGGTAAACTG TCTTGAGCCC 
CATCCTCATC TGCCTGTTTT GGCAACCAGT GGCCTAGACC ATGATGTGAA GATCTGGGCG 
CCGACAGCTG AAGCTTCCAC TGAGCTGACA GGGTTAAAGG AAGTGATCAA GAAGAACAAG 
CGAGAGCGGG ATGAAGACAG CTTGCACCAC ACTGATCTGT TTGATAGTCA CATGCTGTGG 
TTCCTTATGC ATCACCTGAG ACAGAGACGC CATCATCGGC GCTGGCGAGA ACCTGGGGTT 
GGGGCCACAG ATGCAGACTC TGATGAGTCT CCCAGCTCCT CAGACACATC CGACGAGGAG 
GAGGGCCCTG ACCGGGTGCA GTGCATGCCA TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcaf8 ( NM_001357204.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344133.1 Mus musculus DDB1 and CUL4 associated factor 8 (Dcaf8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu02851
Clone ID Related Accession (Same CDS sequence)	XM_006497065.3 , XM_011238889.2 , NM_153555.2 , XM_011238889.3 , NM_001357204.1
Accession Version	NM_001357204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	DDB1- and CUL4-associated factor 8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC074310.29. On Oct 24, 2017 this sequence version replaced XM_006497065.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.195019.1, SRR1660817.270758.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAATA AACGTCCCAA CACGACAGAT GGGAGAACAG ATTTAGCTAA TGGAAGTCTG 
TCCAGCAGCC CAGAGGAGAT GTCTGGAGCT GAAGAGGGGA GGGAAACATC CTCAGGGATT 
GAAGTAGAGG CATCAGACCT GAGTCTCAGC TTGACTGGGG ATGACGGTGG CCCCAACCGC 
ACCAGCACGG AAAGTCGTGG CACAGACACA GAGAGCTCAG GGGAAGAAAA GGACTCTGAC 
AGCATGGAGG ACACGGGCCA TTATTCCATC AATGATGAAA GCCGAGGTCA TGGCCACTCA 
GACGAAGAGG ACGAGGAACA ACCTCGGCAC CGTGGACAGC GCAAACGGGC CAGCCGTGAC 
CAGGATTCAT CAGATGATGA GCGAGCCCTG GAAGACTGGG TATCTTCAGA GACAACAGCC 
TTACCCCGAC CTCGCTGGCA AGCCCTTCCT GCGCTTCGGG AGCGGGAACT GGGTTCCAGT 
GCCCGCTTTG TGTACGAGGC TTGTGGAGCT AGAGTCTTTG TACAGCGTTT CCGTTTGCAG 
CACGGGCTTG AGGGCCATAC TGGTTGTGTC AATACCTTGC ACTTTAACCA GCGTGGTACC 
TGGCTGGCCA GTGGCAGTGA TGACCTGAAG GTGGTGGTGT GGGATTGGGT GCGGCGGCAG 
CCAGTACTGG ACTTTGAGAG TGGCCACAAA AGTAATGTCT TCCAGGCCAA GTTCCTTCCT 
AACAGTGGTG ACTCCACCCT GGCTATGTGT GCCCGGGATG GACAGGTCCG AGTCGCAGAG 
CTGTCTGCTA CACAGTGCTG CAAGAACACC AAGCGTGTGG CCCAGCACAA GGGGGCATCT 
CACAAGTTAG CCCTAGAACC AGACTCCCCC TGCACGTTCC TGTCAGCTGG TGAAGATGCC 
GTCGTCTTCA CCATTGACCT GAGACAAGAC CGGCCAGCTT CGAAACTAGT GGTGACAAAA 
GAGAAGGAGA AGAAAGTGGG GCTGTATACA ATCTATGTGA ACCCTGCCAA TACCCACCAG 
TTTGCAGTGG GTGGAAGAGA TCAGTATGTA AGGATTTATG ACCAGAGGAA AATTGATGAG 
AATGAGAACA ACGGAGTCCT GAAAAAATTC TGTCCTCACC ACTTGGTGAA CAGTGAGTCC 
AAAGCAAACA TCACCTGTCT TGTGTACAGC CACGACGGCA CAGAGCTCCT GGCCAGTTAC 
AATGATGAAG ACATTTACCT TTTCAACTCT TCTCACAGTG ATGGGGCCCA GTATATTAAG 
AGATACAAAG GCCATAGAAA TAATGCTACA GTAAAAGGCG TCAATTTCTA TGGCCCCAAG 
AGTGAGTTTG TGGTGAGCGG TAGTGACTGC GGGCACATCT TCCTCTGGGA GAAGTCATCT 
TGCCAGATTA TTCAGTTCAT GGAAGGGGAC AAGGGAGGTG TGGTAAACTG TCTTGAGCCC 
CATCCTCATC TGCCTGTTTT GGCAACCAGT GGCCTAGACC ATGATGTGAA GATCTGGGCG 
CCGACAGCTG AAGCTTCCAC TGAGCTGACA GGGTTAAAGG AAGTGATCAA GAAGAACAAG 
CGAGAGCGGG ATGAAGACAG CTTGCACCAC ACTGATCTGT TTGATAGTCA CATGCTGTGG 
TTCCTTATGC ATCACCTGAG ACAGAGACGC CATCATCGGC GCTGGCGAGA ACCTGGGGTT 
GGGGCCACAG ATGCAGACTC TGATGAGTCT CCCAGCTCCT CAGACACATC CGACGAGGAG 
GAGGGCCCTG ACCGGGTGCA GTGCATGCCA TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344133.1
CDS	417..2192
Translation	MSNKRPNTTDGRTDLANGSLSSSPEEMSGAEEGRETSSGIEVEASDLSLSLTGDDGGPNRTSTESRGTDTESSGEEKDSDSMEDTGHYSINDESRGHGHSDEEDEEQPRHRGQRKRASRDQDSSDDERALEDWVSSETTALPRPRWQALPALRERELGSSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKVVVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSATQCCKNTKRVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVGLYTIYVNPANTHQFAVGGRDQYVRIYDQRKIDENENNGVLKKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYIKRYKGHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKEVIKKNKRERDEDSLHHTDLFDSHMLWFLMHHLRQRRHHRRWREPGVGATDADSDESPSSSDTSDEEEGPDRVQCMPS

Target ORF information:

RefSeq Version	NM_001357204.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DDB1 and CUL4 associated factor 8 (Dcaf8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357204.1

ORF Insert Sequence:

ATGTCCAATA AACGTCCCAA CACGACAGAT GGGAGAACAG ATTTAGCTAA TGGAAGTCTG 
TCCAGCAGCC CAGAGGAGAT GTCTGGAGCT GAAGAGGGGA GGGAAACATC CTCAGGGATT 
GAAGTAGAGG CATCAGACCT GAGTCTCAGC TTGACTGGGG ATGACGGTGG CCCCAACCGC 
ACCAGCACGG AAAGTCGTGG CACAGACACA GAGAGCTCAG GGGAAGAAAA GGACTCTGAC 
AGCATGGAGG ACACGGGCCA TTATTCCATC AATGATGAAA GCCGAGGTCA TGGCCACTCA 
GACGAAGAGG ACGAGGAACA ACCTCGGCAC CGTGGACAGC GCAAACGGGC CAGCCGTGAC 
CAGGATTCAT CAGATGATGA GCGAGCCCTG GAAGACTGGG TATCTTCAGA GACAACAGCC 
TTACCCCGAC CTCGCTGGCA AGCCCTTCCT GCGCTTCGGG AGCGGGAACT GGGTTCCAGT 
GCCCGCTTTG TGTACGAGGC TTGTGGAGCT AGAGTCTTTG TACAGCGTTT CCGTTTGCAG 
CACGGGCTTG AGGGCCATAC TGGTTGTGTC AATACCTTGC ACTTTAACCA GCGTGGTACC 
TGGCTGGCCA GTGGCAGTGA TGACCTGAAG GTGGTGGTGT GGGATTGGGT GCGGCGGCAG 
CCAGTACTGG ACTTTGAGAG TGGCCACAAA AGTAATGTCT TCCAGGCCAA GTTCCTTCCT 
AACAGTGGTG ACTCCACCCT GGCTATGTGT GCCCGGGATG GACAGGTCCG AGTCGCAGAG 
CTGTCTGCTA CACAGTGCTG CAAGAACACC AAGCGTGTGG CCCAGCACAA GGGGGCATCT 
CACAAGTTAG CCCTAGAACC AGACTCCCCC TGCACGTTCC TGTCAGCTGG TGAAGATGCC 
GTCGTCTTCA CCATTGACCT GAGACAAGAC CGGCCAGCTT CGAAACTAGT GGTGACAAAA 
GAGAAGGAGA AGAAAGTGGG GCTGTATACA ATCTATGTGA ACCCTGCCAA TACCCACCAG 
TTTGCAGTGG GTGGAAGAGA TCAGTATGTA AGGATTTATG ACCAGAGGAA AATTGATGAG 
AATGAGAACA ACGGAGTCCT GAAAAAATTC TGTCCTCACC ACTTGGTGAA CAGTGAGTCC 
AAAGCAAACA TCACCTGTCT TGTGTACAGC CACGACGGCA CAGAGCTCCT GGCCAGTTAC 
AATGATGAAG ACATTTACCT TTTCAACTCT TCTCACAGTG ATGGGGCCCA GTATATTAAG 
AGATACAAAG GCCATAGAAA TAATGCTACA GTAAAAGGCG TCAATTTCTA TGGCCCCAAG 
AGTGAGTTTG TGGTGAGCGG TAGTGACTGC GGGCACATCT TCCTCTGGGA GAAGTCATCT 
TGCCAGATTA TTCAGTTCAT GGAAGGGGAC AAGGGAGGTG TGGTAAACTG TCTTGAGCCC 
CATCCTCATC TGCCTGTTTT GGCAACCAGT GGCCTAGACC ATGATGTGAA GATCTGGGCG 
CCGACAGCTG AAGCTTCCAC TGAGCTGACA GGGTTAAAGG AAGTGATCAA GAAGAACAAG 
CGAGAGCGGG ATGAAGACAG CTTGCACCAC ACTGATCTGT TTGATAGTCA CATGCTGTGG 
TTCCTTATGC ATCACCTGAG ACAGAGACGC CATCATCGGC GCTGGCGAGA ACCTGGGGTT 
GGGGCCACAG ATGCAGACTC TGATGAGTCT CCCAGCTCCT CAGACACATC CGACGAGGAG 
GAGGGCCCTG ACCGGGTGCA GTGCATGCCA TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcaf8 ( XM_006497065.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497128.1 Mus musculus DDB1 and CUL4 associated factor 8 (Dcaf8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu02851
Clone ID Related Accession (Same CDS sequence)	XM_006497065.3 , XM_011238889.2 , NM_153555.2 , XM_011238889.3 , NM_001357204.1
Accession Version	XM_006497065.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	DDB1- and CUL4-associated factor 8 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497065.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAATA AACGTCCCAA CACGACAGAT GGGAGAACAG ATTTAGCTAA TGGAAGTCTG 
TCCAGCAGCC CAGAGGAGAT GTCTGGAGCT GAAGAGGGGA GGGAAACATC CTCAGGGATT 
GAAGTAGAGG CATCAGACCT GAGTCTCAGC TTGACTGGGG ATGACGGTGG CCCCAACCGC 
ACCAGCACGG AAAGTCGTGG CACAGACACA GAGAGCTCAG GGGAAGAAAA GGACTCTGAC 
AGCATGGAGG ACACGGGCCA TTATTCCATC AATGATGAAA GCCGAGGTCA TGGCCACTCA 
GACGAAGAGG ACGAGGAACA ACCTCGGCAC CGTGGACAGC GCAAACGGGC CAGCCGTGAC 
CAGGATTCAT CAGATGATGA GCGAGCCCTG GAAGACTGGG TATCTTCAGA GACAACAGCC 
TTACCCCGAC CTCGCTGGCA AGCCCTTCCT GCGCTTCGGG AGCGGGAACT GGGTTCCAGT 
GCCCGCTTTG TGTACGAGGC TTGTGGAGCT AGAGTCTTTG TACAGCGTTT CCGTTTGCAG 
CACGGGCTTG AGGGCCATAC TGGTTGTGTC AATACCTTGC ACTTTAACCA GCGTGGTACC 
TGGCTGGCCA GTGGCAGTGA TGACCTGAAG GTGGTGGTGT GGGATTGGGT GCGGCGGCAG 
CCAGTACTGG ACTTTGAGAG TGGCCACAAA AGTAATGTCT TCCAGGCCAA GTTCCTTCCT 
AACAGTGGTG ACTCCACCCT GGCTATGTGT GCCCGGGATG GACAGGTCCG AGTCGCAGAG 
CTGTCTGCTA CACAGTGCTG CAAGAACACC AAGCGTGTGG CCCAGCACAA GGGGGCATCT 
CACAAGTTAG CCCTAGAACC AGACTCCCCC TGCACGTTCC TGTCAGCTGG TGAAGATGCC 
GTCGTCTTCA CCATTGACCT GAGACAAGAC CGGCCAGCTT CGAAACTAGT GGTGACAAAA 
GAGAAGGAGA AGAAAGTGGG GCTGTATACA ATCTATGTGA ACCCTGCCAA TACCCACCAG 
TTTGCAGTGG GTGGAAGAGA TCAGTATGTA AGGATTTATG ACCAGAGGAA AATTGATGAG 
AATGAGAACA ACGGAGTCCT GAAAAAATTC TGTCCTCACC ACTTGGTGAA CAGTGAGTCC 
AAAGCAAACA TCACCTGTCT TGTGTACAGC CACGACGGCA CAGAGCTCCT GGCCAGTTAC 
AATGATGAAG ACATTTACCT TTTCAACTCT TCTCACAGTG ATGGGGCCCA GTATATTAAG 
AGATACAAAG GCCATAGAAA TAATGCTACA GTAAAAGGCG TCAATTTCTA TGGCCCCAAG 
AGTGAGTTTG TGGTGAGCGG TAGTGACTGC GGGCACATCT TCCTCTGGGA GAAGTCATCT 
TGCCAGATTA TTCAGTTCAT GGAAGGGGAC AAGGGAGGTG TGGTAAACTG TCTTGAGCCC 
CATCCTCATC TGCCTGTTTT GGCAACCAGT GGCCTAGACC ATGATGTGAA GATCTGGGCG 
CCGACAGCTG AAGCTTCCAC TGAGCTGACA GGGTTAAAGG AAGTGATCAA GAAGAACAAG 
CGAGAGCGGG ATGAAGACAG CTTGCACCAC ACTGATCTGT TTGATAGTCA CATGCTGTGG 
TTCCTTATGC ATCACCTGAG ACAGAGACGC CATCATCGGC GCTGGCGAGA ACCTGGGGTT 
GGGGCCACAG ATGCAGACTC TGATGAGTCT CCCAGCTCCT CAGACACATC CGACGAGGAG 
GAGGGCCCTG ACCGGGTGCA GTGCATGCCA TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497128.1
CDS	406..2181
Translation	MSNKRPNTTDGRTDLANGSLSSSPEEMSGAEEGRETSSGIEVEASDLSLSLTGDDGGPNRTSTESRGTDTESSGEEKDSDSMEDTGHYSINDESRGHGHSDEEDEEQPRHRGQRKRASRDQDSSDDERALEDWVSSETTALPRPRWQALPALRERELGSSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKVVVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSATQCCKNTKRVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVGLYTIYVNPANTHQFAVGGRDQYVRIYDQRKIDENENNGVLKKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYIKRYKGHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKEVIKKNKRERDEDSLHHTDLFDSHMLWFLMHHLRQRRHHRRWREPGVGATDADSDESPSSSDTSDEEEGPDRVQCMPS

Target ORF information:

RefSeq Version	XM_006497065.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus DDB1 and CUL4 associated factor 8 (Dcaf8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497065.3

ORF Insert Sequence:

ATGTCCAATA AACGTCCCAA CACGACAGAT GGGAGAACAG ATTTAGCTAA TGGAAGTCTG 
TCCAGCAGCC CAGAGGAGAT GTCTGGAGCT GAAGAGGGGA GGGAAACATC CTCAGGGATT 
GAAGTAGAGG CATCAGACCT GAGTCTCAGC TTGACTGGGG ATGACGGTGG CCCCAACCGC 
ACCAGCACGG AAAGTCGTGG CACAGACACA GAGAGCTCAG GGGAAGAAAA GGACTCTGAC 
AGCATGGAGG ACACGGGCCA TTATTCCATC AATGATGAAA GCCGAGGTCA TGGCCACTCA 
GACGAAGAGG ACGAGGAACA ACCTCGGCAC CGTGGACAGC GCAAACGGGC CAGCCGTGAC 
CAGGATTCAT CAGATGATGA GCGAGCCCTG GAAGACTGGG TATCTTCAGA GACAACAGCC 
TTACCCCGAC CTCGCTGGCA AGCCCTTCCT GCGCTTCGGG AGCGGGAACT GGGTTCCAGT 
GCCCGCTTTG TGTACGAGGC TTGTGGAGCT AGAGTCTTTG TACAGCGTTT CCGTTTGCAG 
CACGGGCTTG AGGGCCATAC TGGTTGTGTC AATACCTTGC ACTTTAACCA GCGTGGTACC 
TGGCTGGCCA GTGGCAGTGA TGACCTGAAG GTGGTGGTGT GGGATTGGGT GCGGCGGCAG 
CCAGTACTGG ACTTTGAGAG TGGCCACAAA AGTAATGTCT TCCAGGCCAA GTTCCTTCCT 
AACAGTGGTG ACTCCACCCT GGCTATGTGT GCCCGGGATG GACAGGTCCG AGTCGCAGAG 
CTGTCTGCTA CACAGTGCTG CAAGAACACC AAGCGTGTGG CCCAGCACAA GGGGGCATCT 
CACAAGTTAG CCCTAGAACC AGACTCCCCC TGCACGTTCC TGTCAGCTGG TGAAGATGCC 
GTCGTCTTCA CCATTGACCT GAGACAAGAC CGGCCAGCTT CGAAACTAGT GGTGACAAAA 
GAGAAGGAGA AGAAAGTGGG GCTGTATACA ATCTATGTGA ACCCTGCCAA TACCCACCAG 
TTTGCAGTGG GTGGAAGAGA TCAGTATGTA AGGATTTATG ACCAGAGGAA AATTGATGAG 
AATGAGAACA ACGGAGTCCT GAAAAAATTC TGTCCTCACC ACTTGGTGAA CAGTGAGTCC 
AAAGCAAACA TCACCTGTCT TGTGTACAGC CACGACGGCA CAGAGCTCCT GGCCAGTTAC 
AATGATGAAG ACATTTACCT TTTCAACTCT TCTCACAGTG ATGGGGCCCA GTATATTAAG 
AGATACAAAG GCCATAGAAA TAATGCTACA GTAAAAGGCG TCAATTTCTA TGGCCCCAAG 
AGTGAGTTTG TGGTGAGCGG TAGTGACTGC GGGCACATCT TCCTCTGGGA GAAGTCATCT 
TGCCAGATTA TTCAGTTCAT GGAAGGGGAC AAGGGAGGTG TGGTAAACTG TCTTGAGCCC 
CATCCTCATC TGCCTGTTTT GGCAACCAGT GGCCTAGACC ATGATGTGAA GATCTGGGCG 
CCGACAGCTG AAGCTTCCAC TGAGCTGACA GGGTTAAAGG AAGTGATCAA GAAGAACAAG 
CGAGAGCGGG ATGAAGACAG CTTGCACCAC ACTGATCTGT TTGATAGTCA CATGCTGTGG 
TTCCTTATGC ATCACCTGAG ACAGAGACGC CATCATCGGC GCTGGCGAGA ACCTGGGGTT 
GGGGCCACAG ATGCAGACTC TGATGAGTCT CCCAGCTCCT CAGACACATC CGACGAGGAG 
GAGGGCCCTG ACCGGGTGCA GTGCATGCCA TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcaf8 ( XM_011238889.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237191.1 Mus musculus DDB1 and CUL4 associated factor 8 (Dcaf8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu02851
Clone ID Related Accession (Same CDS sequence)	XM_006497065.3 , XM_011238889.2 , NM_153555.2 , XM_011238889.3 , NM_001357204.1
Accession Version	XM_011238889.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DDB1- and CUL4-associated factor 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAATA AACGTCCCAA CACGACAGAT GGGAGAACAG ATTTAGCTAA TGGAAGTCTG 
TCCAGCAGCC CAGAGGAGAT GTCTGGAGCT GAAGAGGGGA GGGAAACATC CTCAGGGATT 
GAAGTAGAGG CATCAGACCT GAGTCTCAGC TTGACTGGGG ATGACGGTGG CCCCAACCGC 
ACCAGCACGG AAAGTCGTGG CACAGACACA GAGAGCTCAG GGGAAGAAAA GGACTCTGAC 
AGCATGGAGG ACACGGGCCA TTATTCCATC AATGATGAAA GCCGAGGTCA TGGCCACTCA 
GACGAAGAGG ACGAGGAACA ACCTCGGCAC CGTGGACAGC GCAAACGGGC CAGCCGTGAC 
CAGGATTCAT CAGATGATGA GCGAGCCCTG GAAGACTGGG TATCTTCAGA GACAACAGCC 
TTACCCCGAC CTCGCTGGCA AGCCCTTCCT GCGCTTCGGG AGCGGGAACT GGGTTCCAGT 
GCCCGCTTTG TGTACGAGGC TTGTGGAGCT AGAGTCTTTG TACAGCGTTT CCGTTTGCAG 
CACGGGCTTG AGGGCCATAC TGGTTGTGTC AATACCTTGC ACTTTAACCA GCGTGGTACC 
TGGCTGGCCA GTGGCAGTGA TGACCTGAAG GTGGTGGTGT GGGATTGGGT GCGGCGGCAG 
CCAGTACTGG ACTTTGAGAG TGGCCACAAA AGTAATGTCT TCCAGGCCAA GTTCCTTCCT 
AACAGTGGTG ACTCCACCCT GGCTATGTGT GCCCGGGATG GACAGGTCCG AGTCGCAGAG 
CTGTCTGCTA CACAGTGCTG CAAGAACACC AAGCGTGTGG CCCAGCACAA GGGGGCATCT 
CACAAGTTAG CCCTAGAACC AGACTCCCCC TGCACGTTCC TGTCAGCTGG TGAAGATGCC 
GTCGTCTTCA CCATTGACCT GAGACAAGAC CGGCCAGCTT CGAAACTAGT GGTGACAAAA 
GAGAAGGAGA AGAAAGTGGG GCTGTATACA ATCTATGTGA ACCCTGCCAA TACCCACCAG 
TTTGCAGTGG GTGGAAGAGA TCAGTATGTA AGGATTTATG ACCAGAGGAA AATTGATGAG 
AATGAGAACA ACGGAGTCCT GAAAAAATTC TGTCCTCACC ACTTGGTGAA CAGTGAGTCC 
AAAGCAAACA TCACCTGTCT TGTGTACAGC CACGACGGCA CAGAGCTCCT GGCCAGTTAC 
AATGATGAAG ACATTTACCT TTTCAACTCT TCTCACAGTG ATGGGGCCCA GTATATTAAG 
AGATACAAAG GCCATAGAAA TAATGCTACA GTAAAAGGCG TCAATTTCTA TGGCCCCAAG 
AGTGAGTTTG TGGTGAGCGG TAGTGACTGC GGGCACATCT TCCTCTGGGA GAAGTCATCT 
TGCCAGATTA TTCAGTTCAT GGAAGGGGAC AAGGGAGGTG TGGTAAACTG TCTTGAGCCC 
CATCCTCATC TGCCTGTTTT GGCAACCAGT GGCCTAGACC ATGATGTGAA GATCTGGGCG 
CCGACAGCTG AAGCTTCCAC TGAGCTGACA GGGTTAAAGG AAGTGATCAA GAAGAACAAG 
CGAGAGCGGG ATGAAGACAG CTTGCACCAC ACTGATCTGT TTGATAGTCA CATGCTGTGG 
TTCCTTATGC ATCACCTGAG ACAGAGACGC CATCATCGGC GCTGGCGAGA ACCTGGGGTT 
GGGGCCACAG ATGCAGACTC TGATGAGTCT CCCAGCTCCT CAGACACATC CGACGAGGAG 
GAGGGCCCTG ACCGGGTGCA GTGCATGCCA TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237191.1
CDS	1189..2964
Translation	MSNKRPNTTDGRTDLANGSLSSSPEEMSGAEEGRETSSGIEVEASDLSLSLTGDDGGPNRTSTESRGTDTESSGEEKDSDSMEDTGHYSINDESRGHGHSDEEDEEQPRHRGQRKRASRDQDSSDDERALEDWVSSETTALPRPRWQALPALRERELGSSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKVVVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSATQCCKNTKRVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVGLYTIYVNPANTHQFAVGGRDQYVRIYDQRKIDENENNGVLKKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYIKRYKGHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKEVIKKNKRERDEDSLHHTDLFDSHMLWFLMHHLRQRRHHRRWREPGVGATDADSDESPSSSDTSDEEEGPDRVQCMPS

Target ORF information:

RefSeq Version	XM_011238889.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DDB1 and CUL4 associated factor 8 (Dcaf8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238889.2

ORF Insert Sequence:

ATGTCCAATA AACGTCCCAA CACGACAGAT GGGAGAACAG ATTTAGCTAA TGGAAGTCTG 
TCCAGCAGCC CAGAGGAGAT GTCTGGAGCT GAAGAGGGGA GGGAAACATC CTCAGGGATT 
GAAGTAGAGG CATCAGACCT GAGTCTCAGC TTGACTGGGG ATGACGGTGG CCCCAACCGC 
ACCAGCACGG AAAGTCGTGG CACAGACACA GAGAGCTCAG GGGAAGAAAA GGACTCTGAC 
AGCATGGAGG ACACGGGCCA TTATTCCATC AATGATGAAA GCCGAGGTCA TGGCCACTCA 
GACGAAGAGG ACGAGGAACA ACCTCGGCAC CGTGGACAGC GCAAACGGGC CAGCCGTGAC 
CAGGATTCAT CAGATGATGA GCGAGCCCTG GAAGACTGGG TATCTTCAGA GACAACAGCC 
TTACCCCGAC CTCGCTGGCA AGCCCTTCCT GCGCTTCGGG AGCGGGAACT GGGTTCCAGT 
GCCCGCTTTG TGTACGAGGC TTGTGGAGCT AGAGTCTTTG TACAGCGTTT CCGTTTGCAG 
CACGGGCTTG AGGGCCATAC TGGTTGTGTC AATACCTTGC ACTTTAACCA GCGTGGTACC 
TGGCTGGCCA GTGGCAGTGA TGACCTGAAG GTGGTGGTGT GGGATTGGGT GCGGCGGCAG 
CCAGTACTGG ACTTTGAGAG TGGCCACAAA AGTAATGTCT TCCAGGCCAA GTTCCTTCCT 
AACAGTGGTG ACTCCACCCT GGCTATGTGT GCCCGGGATG GACAGGTCCG AGTCGCAGAG 
CTGTCTGCTA CACAGTGCTG CAAGAACACC AAGCGTGTGG CCCAGCACAA GGGGGCATCT 
CACAAGTTAG CCCTAGAACC AGACTCCCCC TGCACGTTCC TGTCAGCTGG TGAAGATGCC 
GTCGTCTTCA CCATTGACCT GAGACAAGAC CGGCCAGCTT CGAAACTAGT GGTGACAAAA 
GAGAAGGAGA AGAAAGTGGG GCTGTATACA ATCTATGTGA ACCCTGCCAA TACCCACCAG 
TTTGCAGTGG GTGGAAGAGA TCAGTATGTA AGGATTTATG ACCAGAGGAA AATTGATGAG 
AATGAGAACA ACGGAGTCCT GAAAAAATTC TGTCCTCACC ACTTGGTGAA CAGTGAGTCC 
AAAGCAAACA TCACCTGTCT TGTGTACAGC CACGACGGCA CAGAGCTCCT GGCCAGTTAC 
AATGATGAAG ACATTTACCT TTTCAACTCT TCTCACAGTG ATGGGGCCCA GTATATTAAG 
AGATACAAAG GCCATAGAAA TAATGCTACA GTAAAAGGCG TCAATTTCTA TGGCCCCAAG 
AGTGAGTTTG TGGTGAGCGG TAGTGACTGC GGGCACATCT TCCTCTGGGA GAAGTCATCT 
TGCCAGATTA TTCAGTTCAT GGAAGGGGAC AAGGGAGGTG TGGTAAACTG TCTTGAGCCC 
CATCCTCATC TGCCTGTTTT GGCAACCAGT GGCCTAGACC ATGATGTGAA GATCTGGGCG 
CCGACAGCTG AAGCTTCCAC TGAGCTGACA GGGTTAAAGG AAGTGATCAA GAAGAACAAG 
CGAGAGCGGG ATGAAGACAG CTTGCACCAC ACTGATCTGT TTGATAGTCA CATGCTGTGG 
TTCCTTATGC ATCACCTGAG ACAGAGACGC CATCATCGGC GCTGGCGAGA ACCTGGGGTT 
GGGGCCACAG ATGCAGACTC TGATGAGTCT CCCAGCTCCT CAGACACATC CGACGAGGAG 
GAGGGCCCTG ACCGGGTGCA GTGCATGCCA TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcaf8 ( XM_011238889.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237191.1 Mus musculus DDB1 and CUL4 associated factor 8 (Dcaf8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu02851
Clone ID Related Accession (Same CDS sequence)	XM_006497065.3 , XM_011238889.2 , NM_153555.2 , XM_011238889.3 , NM_001357204.1
Accession Version	XM_011238889.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DDB1- and CUL4-associated factor 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238889.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAATA AACGTCCCAA CACGACAGAT GGGAGAACAG ATTTAGCTAA TGGAAGTCTG 
TCCAGCAGCC CAGAGGAGAT GTCTGGAGCT GAAGAGGGGA GGGAAACATC CTCAGGGATT 
GAAGTAGAGG CATCAGACCT GAGTCTCAGC TTGACTGGGG ATGACGGTGG CCCCAACCGC 
ACCAGCACGG AAAGTCGTGG CACAGACACA GAGAGCTCAG GGGAAGAAAA GGACTCTGAC 
AGCATGGAGG ACACGGGCCA TTATTCCATC AATGATGAAA GCCGAGGTCA TGGCCACTCA 
GACGAAGAGG ACGAGGAACA ACCTCGGCAC CGTGGACAGC GCAAACGGGC CAGCCGTGAC 
CAGGATTCAT CAGATGATGA GCGAGCCCTG GAAGACTGGG TATCTTCAGA GACAACAGCC 
TTACCCCGAC CTCGCTGGCA AGCCCTTCCT GCGCTTCGGG AGCGGGAACT GGGTTCCAGT 
GCCCGCTTTG TGTACGAGGC TTGTGGAGCT AGAGTCTTTG TACAGCGTTT CCGTTTGCAG 
CACGGGCTTG AGGGCCATAC TGGTTGTGTC AATACCTTGC ACTTTAACCA GCGTGGTACC 
TGGCTGGCCA GTGGCAGTGA TGACCTGAAG GTGGTGGTGT GGGATTGGGT GCGGCGGCAG 
CCAGTACTGG ACTTTGAGAG TGGCCACAAA AGTAATGTCT TCCAGGCCAA GTTCCTTCCT 
AACAGTGGTG ACTCCACCCT GGCTATGTGT GCCCGGGATG GACAGGTCCG AGTCGCAGAG 
CTGTCTGCTA CACAGTGCTG CAAGAACACC AAGCGTGTGG CCCAGCACAA GGGGGCATCT 
CACAAGTTAG CCCTAGAACC AGACTCCCCC TGCACGTTCC TGTCAGCTGG TGAAGATGCC 
GTCGTCTTCA CCATTGACCT GAGACAAGAC CGGCCAGCTT CGAAACTAGT GGTGACAAAA 
GAGAAGGAGA AGAAAGTGGG GCTGTATACA ATCTATGTGA ACCCTGCCAA TACCCACCAG 
TTTGCAGTGG GTGGAAGAGA TCAGTATGTA AGGATTTATG ACCAGAGGAA AATTGATGAG 
AATGAGAACA ACGGAGTCCT GAAAAAATTC TGTCCTCACC ACTTGGTGAA CAGTGAGTCC 
AAAGCAAACA TCACCTGTCT TGTGTACAGC CACGACGGCA CAGAGCTCCT GGCCAGTTAC 
AATGATGAAG ACATTTACCT TTTCAACTCT TCTCACAGTG ATGGGGCCCA GTATATTAAG 
AGATACAAAG GCCATAGAAA TAATGCTACA GTAAAAGGCG TCAATTTCTA TGGCCCCAAG 
AGTGAGTTTG TGGTGAGCGG TAGTGACTGC GGGCACATCT TCCTCTGGGA GAAGTCATCT 
TGCCAGATTA TTCAGTTCAT GGAAGGGGAC AAGGGAGGTG TGGTAAACTG TCTTGAGCCC 
CATCCTCATC TGCCTGTTTT GGCAACCAGT GGCCTAGACC ATGATGTGAA GATCTGGGCG 
CCGACAGCTG AAGCTTCCAC TGAGCTGACA GGGTTAAAGG AAGTGATCAA GAAGAACAAG 
CGAGAGCGGG ATGAAGACAG CTTGCACCAC ACTGATCTGT TTGATAGTCA CATGCTGTGG 
TTCCTTATGC ATCACCTGAG ACAGAGACGC CATCATCGGC GCTGGCGAGA ACCTGGGGTT 
GGGGCCACAG ATGCAGACTC TGATGAGTCT CCCAGCTCCT CAGACACATC CGACGAGGAG 
GAGGGCCCTG ACCGGGTGCA GTGCATGCCA TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237191.1
CDS	1185..2960
Translation	MSNKRPNTTDGRTDLANGSLSSSPEEMSGAEEGRETSSGIEVEASDLSLSLTGDDGGPNRTSTESRGTDTESSGEEKDSDSMEDTGHYSINDESRGHGHSDEEDEEQPRHRGQRKRASRDQDSSDDERALEDWVSSETTALPRPRWQALPALRERELGSSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKVVVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSATQCCKNTKRVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVGLYTIYVNPANTHQFAVGGRDQYVRIYDQRKIDENENNGVLKKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYIKRYKGHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKEVIKKNKRERDEDSLHHTDLFDSHMLWFLMHHLRQRRHHRRWREPGVGATDADSDESPSSSDTSDEEEGPDRVQCMPS

Target ORF information:

RefSeq Version	XM_011238889.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DDB1 and CUL4 associated factor 8 (Dcaf8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238889.3

ORF Insert Sequence:

ATGTCCAATA AACGTCCCAA CACGACAGAT GGGAGAACAG ATTTAGCTAA TGGAAGTCTG 
TCCAGCAGCC CAGAGGAGAT GTCTGGAGCT GAAGAGGGGA GGGAAACATC CTCAGGGATT 
GAAGTAGAGG CATCAGACCT GAGTCTCAGC TTGACTGGGG ATGACGGTGG CCCCAACCGC 
ACCAGCACGG AAAGTCGTGG CACAGACACA GAGAGCTCAG GGGAAGAAAA GGACTCTGAC 
AGCATGGAGG ACACGGGCCA TTATTCCATC AATGATGAAA GCCGAGGTCA TGGCCACTCA 
GACGAAGAGG ACGAGGAACA ACCTCGGCAC CGTGGACAGC GCAAACGGGC CAGCCGTGAC 
CAGGATTCAT CAGATGATGA GCGAGCCCTG GAAGACTGGG TATCTTCAGA GACAACAGCC 
TTACCCCGAC CTCGCTGGCA AGCCCTTCCT GCGCTTCGGG AGCGGGAACT GGGTTCCAGT 
GCCCGCTTTG TGTACGAGGC TTGTGGAGCT AGAGTCTTTG TACAGCGTTT CCGTTTGCAG 
CACGGGCTTG AGGGCCATAC TGGTTGTGTC AATACCTTGC ACTTTAACCA GCGTGGTACC 
TGGCTGGCCA GTGGCAGTGA TGACCTGAAG GTGGTGGTGT GGGATTGGGT GCGGCGGCAG 
CCAGTACTGG ACTTTGAGAG TGGCCACAAA AGTAATGTCT TCCAGGCCAA GTTCCTTCCT 
AACAGTGGTG ACTCCACCCT GGCTATGTGT GCCCGGGATG GACAGGTCCG AGTCGCAGAG 
CTGTCTGCTA CACAGTGCTG CAAGAACACC AAGCGTGTGG CCCAGCACAA GGGGGCATCT 
CACAAGTTAG CCCTAGAACC AGACTCCCCC TGCACGTTCC TGTCAGCTGG TGAAGATGCC 
GTCGTCTTCA CCATTGACCT GAGACAAGAC CGGCCAGCTT CGAAACTAGT GGTGACAAAA 
GAGAAGGAGA AGAAAGTGGG GCTGTATACA ATCTATGTGA ACCCTGCCAA TACCCACCAG 
TTTGCAGTGG GTGGAAGAGA TCAGTATGTA AGGATTTATG ACCAGAGGAA AATTGATGAG 
AATGAGAACA ACGGAGTCCT GAAAAAATTC TGTCCTCACC ACTTGGTGAA CAGTGAGTCC 
AAAGCAAACA TCACCTGTCT TGTGTACAGC CACGACGGCA CAGAGCTCCT GGCCAGTTAC 
AATGATGAAG ACATTTACCT TTTCAACTCT TCTCACAGTG ATGGGGCCCA GTATATTAAG 
AGATACAAAG GCCATAGAAA TAATGCTACA GTAAAAGGCG TCAATTTCTA TGGCCCCAAG 
AGTGAGTTTG TGGTGAGCGG TAGTGACTGC GGGCACATCT TCCTCTGGGA GAAGTCATCT 
TGCCAGATTA TTCAGTTCAT GGAAGGGGAC AAGGGAGGTG TGGTAAACTG TCTTGAGCCC 
CATCCTCATC TGCCTGTTTT GGCAACCAGT GGCCTAGACC ATGATGTGAA GATCTGGGCG 
CCGACAGCTG AAGCTTCCAC TGAGCTGACA GGGTTAAAGG AAGTGATCAA GAAGAACAAG 
CGAGAGCGGG ATGAAGACAG CTTGCACCAC ACTGATCTGT TTGATAGTCA CATGCTGTGG 
TTCCTTATGC ATCACCTGAG ACAGAGACGC CATCATCGGC GCTGGCGAGA ACCTGGGGTT 
GGGGCCACAG ATGCAGACTC TGATGAGTCT CCCAGCTCCT CAGACACATC CGACGAGGAG 
GAGGGCCCTG ACCGGGTGCA GTGCATGCCA TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CRL4
Cell reports18(6)1499-1511(2017 Feb)
Li G,Ji T,Chen J,Fu Y,Hou L,Feng Y,Zhang T,Song T,Zhao J,Endo Y,Lin H,Cai X,Cang Y

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
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Severe neural tube defects in the loop-tail mouse result from mutation of Lpp1, a novel gene involved in floor plate specification.
Human molecular genetics10(22)2593-601(2001 Oct)
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Ltap, a mammalian homolog of Drosophila Strabismus/Van Gogh, is altered in the mouse neural tube mutant Loop-tail.
Nature genetics28(3)251-5(2001 Jul)
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Dcaf8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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