Tmem132a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tmem132a
Entrez Gene ID	98170
Full Name	transmembrane protein 132A
Synonyms	6720481D13Rik,Hspa5bp1,Orai1,R74613
General protein information	Preferred Names transmembrane protein 132A Names transmembrane protein 132A HSPA5-binding protein 1 heat shock 70kDa protein 5 binding protein 1 heat shock protein 5 binding protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 A

mRNA and Protein(s)

mRNA	Protein	Name
XM_006527503.1	XP_006527566.1	transmembrane protein 132A isoform X1
XM_006527503.2	XP_006527566.1	transmembrane protein 132A isoform X1
NM_133804.2	NP_598565.2	transmembrane protein 132A precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	TMEM132A	NP_060340.2
Bos taurus (cattle)	TMEM132A	NP_001095707.1
Mus musculus (house mouse)	Tmem132a	NP_598565.2
Xenopus tropicalis (tropical clawed frog)	tmem132a	XP_002942160.1
Pan troglodytes (chimpanzee)	TMEM132A	XP_522023.3
Rattus norvegicus (Norway rat)	Tmem132a	NP_821140.1
Canis lupus familiaris (dog)	TMEM132A	XP_855179.2

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Transcriptional and post-transcriptional regulation of transmembrane protein 132A.
Oh-Hashi K, Sone A, Hikiji T, Hirata Y, Vitiello M, Fedele M, Ishigaki S, Sobue G, Kiuchi K
Molecular and cellular biochemistry405(1-2)291-9(2015 Jul)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Characterization of the expression and cell-surface localization of transmembrane protein 132A.
Oh-hashi K, Koga H, Nagase T, Hirata Y, Kiuchi K
Molecular and cellular biochemistry370(1-2)23-33(2012 Nov)

Essential role of the N-terminus of murine Orai1 in store-operated Ca2+ entry.
Takahashi Y, Murakami M, Watanabe H, Hasegawa H, Ohba T, Munehisa Y, Nobori K, Ono K, Iijima T, Ito H
Biochemical and biophysical research communications356(1)45-52(2007 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Markedly attenuated the levels of TMEM132A mRNA.
Title: Transcriptional and post-transcriptional regulation of transmembrane protein 132A.

The novel cell-surface localized glycoprotein, TMEM132A, is regulated by several factors, including GRP78, Sar1, and intracellular calcium, in a post-transcriptional manner.
Title: Characterization of the expression and cell-surface localization of transmembrane protein 132A.

The following Tmem132a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmem132a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu10235	NM_133804.2 Latest version!	Mus musculus transmembrane protein 132A (Tmem132a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$594.30 $849.00
OMu55840	XM_006527503.2 Latest version!	Mus musculus transmembrane protein 132A (Tmem132a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu55840	XM_006527503.1	Mus musculus transmembrane protein 132A (Tmem132a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tmem132a ( NM_133804.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598565.2 Mus musculus transmembrane protein 132A (Tmem132a), mRNA.

Price & Availability↑

CloneID	OMu10235
Clone ID Related Accession (Same CDS sequence)	NM_133804.2
Accession Version	NM_133804.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	transmembrane protein 132A precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK048556.1, AK173207.1 and CF729397.1. On Feb 18, 2006 this sequence version replaced NM_133804.1. ##Evidence-Data-START## Transcript exon combination :: AK032956.1, AK048556.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164136, SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGACTGAAC GCAAGGCTGC GGCCCCTCGG GGGCCCTACG GTGCCTGGTT CTGCCTCCTG 
GTGGCTCTCG CCCTGGAAGT CGTGAGAGTT AGCAGTAACC ATGATACCCT GGACCCCATC 
TACCTGCCAG TGGCCCTGGA ACTCCTAGAT GCCCCTGAGC ATTTTCGTGT GCAGCAGGTG 
GGCCATTACC CACCTGCCAA CTCTTCCCTG GCCTCCCGCT CTGAGACTTT CCTGCTCATG 
CAGCCCTGGC CCAGGGCACA GCCGCTTCTC CGGGCCTCCT ACCCACCCTT TGCCACTCAG 
CAGGTGGTCC CTCCTAGGGT CACCGAGCCC CACCGGCGGC CTGTCCCTTG GGATGTGCGG 
GCTGTTTCCG TGGAAGCAGC GGTGACTCCA GCAGAACCCT ATGCCCGTGT CCTCTTCCAC 
CTCAAAGGGC AGGATTGGCC ACCAGGACCT GGCAGCTTGC CCTGTGCCCG ACTACATGCC 
ACACACCCTG CAGGCACTGC CCACCGAGCC TGCCGCTTCC AGCCATCTCT GGGTGCCTGT 
GTGGTGGAAC TGCAATTCCC CTCTCACTGG TTCTCCCAAA GTGCCACCAC AAGGGCCGAA 
CTGGCCTACA CGCTGGAGCC TGCAGGCGAG GGCCCTGGGG GCTGTGGCCT TGGCACAGAG 
GAGGAACCCA GGGAGCAGGC TCTCCCAGTG GGTGGTGTGG AGCTGCACCC TGAGGACCCC 
CCACAGTACC AGGAGGTACC TCTGGATGAA GCAGTCACTT TGCGTGCACC TGATGTGCCA 
ATGAGGCCTG GCCAGCTCTT CACTGCCACC CTTCTGCTTC GACACAACTT CACTGCGAGC 
CTTCTTACTC TGCGGATTAA GGTGAAGAAA GGGCTGCAGG TGATAGCTGC CCGCCCAGCC 
CAGCCCACCC TCTGGACTGC TAAGTTAGAC CGCTTCAAGG GCTCAAAGCA CCACACCAGC 
CTGATCACCT GTCACCGTGC TGGGCCTGCA GGGCCAGATT CCAGCCCCCT TGAACTGTCC 
GAGTTCCTGT GGGTGGACTT TGCAGTGGAG AACAGCACTG GTGGGGGTGT GGCAGTCACT 
CGCCCTGTCA CGTGGCAGCT GGAGTACCCA GGTCAGGCCC CCGAAGCAGA GAAGGACAAA 
ATGGTGTGGG AGATCCTGGT GTCTGAGCGG GACATCAGAG CCCTCATCCC TCTGGCCAAG 
GCTGAGGAGC TGGTGAACAC GGCTCCATTG ACTGGAGTGC CCCAGCGTAT TCCGGTGCGG 
CTCGTCACTG TGGACAGTGG GGGAGCCTTG GAGGAGGTGA CAGAGCACAT TGGCTGTGAG 
TCAGCCAATA CACAGGTCCT GCAGGTATCT GAGGCCTGTG ATGCTGTGTT CGTGGCTGGC 
CAGGAGAGCC GGGGTGCCAA GGGGGTGAGG GTGGACTTCT GGTGGCGCCG ACTTCGGGCC 
TCACTAAAGC TGACTGTATG GGCCCCACTC CTGCCCTTGC GCATTGAGCT GACTGACACC 
ACTCTGGAAC AAATCCGAGG CTGGAGGGTC CCAGGCTCAG CAGAAGGGCA GCTGGAGCCT 
GAGACTGCTG CTGAGGAGGT GGAGCGGCGC TCCCGTGGCT GCCGCCTGCA GTACCAGAGA 
GCTGGTGTGC GCTTCTTGGT GCCCTTTGCG GCTCACCCGC TGGACGGTGG CCGTCGCCTC 
ACCCACCTAC TCGGTCCTGA CTGGTTGCTG GATGTGAGTC ACCTCGTGGC AGCCCATGCC 
CATGTGCAAG ATCCTCGCAT AGCCTCCTTG GAAGGTGGCC GCATCCTAGT GGGACGGGAG 
CCTGGAGTCA CCTCTATCGA GGTTCGTTCC CCGCTGTCTG ACGCCATCCT GGGGGAGCAG 
GCACTGGCTG TGACAGACGA CAAGGTCTCA GTGCTGGATC TGCGAGTACA GCCGGTGATG 
GGCATCTCAC TGTCCTTGAG CCGGGGCATG TCCCACCCTG GAGAAGTCAC AGCCACATGC 
TGGGCCCAGT CAGCTCTTCC AGCCCCAAAG CAGGAGGTGG CCCTCTCCCT GTGGCTGTCC 
TTCTCTGACC ACACTTTGGC CCCAGCCGAG CTCTACGACC GCAATGACCT GGGGCTGTCG 
GTCTCAGCTG AGGAGCCCAG TGCTGTTCTG CCAGCCGAAG AGCAGGGAGC CCAGCTTGGT 
GTGGTGGTGA GTGGTGTGGG TGCCGAAGGG CTGCCCCTAC ACGTGGCTCT GCACCCACCC 
GAACCCTGCC GCCGGGGCCG CCATCGCGTT CCCCTGGCAT CAGGCACCGC CTGGCTGGGG 
TTGCCCCCTT TGCCCACACC AGTTCCTGCC CTCCCATCCA GCCCTGTCCG GACCTCACCG 
TTCACAGAAG CCACGGTGGA GGGAAAGCGG CAGATAGCAG GTGATATGGG GGGCCATGTG 
GGTATTAGGG GCAAGTTTGA GCGGGCAGAG GAAGAAGCTG GGAAGGAAGA AAATGAGGCC 
AAAGAAGAGG AGGAAGATGA GGAAGAGATG GTGCCTGCCC CTCAGCGTGT CACCGACCTA 
GAGCTGGGGA TGTATGCTCT TCTGGGCATC TTCTGTTTGG CCATCCTCAT CTTCCTGGTC 
AATGGTGTGG TCTTTGTGTT GCGCTACCAG CGCAAAGAGC CTCCAGACAG CGCTACAGAC 
CCTGCCTCCC CTCAGCCACA TAACTGGGTC TGGCTGGGCA CCAATCAGGA GGAACTGAGT 
CGGCAGCTGG ACCGATGCTC TTCCTCGGGT CCACCCAAGG GGGAGGGGGG CTGCCCTTGT 
GAAAGTGGGG CAGGAGGGGA TGCCTCAACC GTGGCCCCCA GTGCTTCAGA GAGCCCTGCT 
GGCTCCTCGA GCACCCTGGC CCGCAAAGAA GCCGGGGGAC GTCGGAAGCG AGTCGAGTTT 
GTGACATTTG CACCAGCACC CCCAACCCAG CCGCCTGAGG AGCCTGTAGG GGCCCCCGCT 
GTACAGTCCA TCCTGGTGGC AGGTGAGGAG GACATCCGCT GGGTGTGCGA GGACATGGGG 
CTGAAGGACC CAGAGGAGCT TCGAAACTAC ATGGAGAGGA TCCGGGGCAG CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598565.2
CDS	154..3210
Translation	MTERKAAAPRGPYGAWFCLLVALALEVVRVSSNHDTLDPIYLPVALELLDAPEHFRVQQVGHYPPANSSLASRSETFLLMQPWPRAQPLLRASYPPFATQQVVPPRVTEPHRRPVPWDVRAVSVEAAVTPAEPYARVLFHLKGQDWPPGPGSLPCARLHATHPAGTAHRACRFQPSLGACVVELQFPSHWFSQSATTRAELAYTLEPAGEGPGGCGLGTEEEPREQALPVGGVELHPEDPPQYQEVPLDEAVTLRAPDVPMRPGQLFTATLLLRHNFTASLLTLRIKVKKGLQVIAARPAQPTLWTAKLDRFKGSKHHTSLITCHRAGPAGPDSSPLELSEFLWVDFAVENSTGGGVAVTRPVTWQLEYPGQAPEAEKDKMVWEILVSERDIRALIPLAKAEELVNTAPLTGVPQRIPVRLVTVDSGGALEEVTEHIGCESANTQVLQVSEACDAVFVAGQESRGAKGVRVDFWWRRLRASLKLTVWAPLLPLRIELTDTTLEQIRGWRVPGSAEGQLEPETAAEEVERRSRGCRLQYQRAGVRFLVPFAAHPLDGGRRLTHLLGPDWLLDVSHLVAAHAHVQDPRIASLEGGRILVGREPGVTSIEVRSPLSDAILGEQALAVTDDKVSVLDLRVQPVMGISLSLSRGMSHPGEVTATCWAQSALPAPKQEVALSLWLSFSDHTLAPAELYDRNDLGLSVSAEEPSAVLPAEEQGAQLGVVVSGVGAEGLPLHVALHPPEPCRRGRHRVPLASGTAWLGLPPLPTPVPALPSSPVRTSPFTEATVEGKRQIAGDMGGHVGIRGKFERAEEEAGKEENEAKEEEEDEEEMVPAPQRVTDLELGMYALLGIFCLAILIFLVNGVVFVLRYQRKEPPDSATDPASPQPHNWVWLGTNQEELSRQLDRCSSSGPPKGEGGCPCESGAGGDASTVAPSASESPAGSSSTLARKEAGGRRKRVEFVTFAPAPPTQPPEEPVGAPAVQSILVAGEEDIRWVCEDMGLKDPEELRNYMERIRGSS

Target ORF information:

RefSeq Version	NM_133804.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein 132A (Tmem132a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133804.2

ORF Insert Sequence:

ATGACTGAAC GCAAGGCTGC GGCCCCTCGG GGGCCCTACG GTGCCTGGTT CTGCCTCCTG 
GTGGCTCTCG CCCTGGAAGT CGTGAGAGTT AGCAGTAACC ATGATACCCT GGACCCCATC 
TACCTGCCAG TGGCCCTGGA ACTCCTAGAT GCCCCTGAGC ATTTTCGTGT GCAGCAGGTG 
GGCCATTACC CACCTGCCAA CTCTTCCCTG GCCTCCCGCT CTGAGACTTT CCTGCTCATG 
CAGCCCTGGC CCAGGGCACA GCCGCTTCTC CGGGCCTCCT ACCCACCCTT TGCCACTCAG 
CAGGTGGTCC CTCCTAGGGT CACCGAGCCC CACCGGCGGC CTGTCCCTTG GGATGTGCGG 
GCTGTTTCCG TGGAAGCAGC GGTGACTCCA GCAGAACCCT ATGCCCGTGT CCTCTTCCAC 
CTCAAAGGGC AGGATTGGCC ACCAGGACCT GGCAGCTTGC CCTGTGCCCG ACTACATGCC 
ACACACCCTG CAGGCACTGC CCACCGAGCC TGCCGCTTCC AGCCATCTCT GGGTGCCTGT 
GTGGTGGAAC TGCAATTCCC CTCTCACTGG TTCTCCCAAA GTGCCACCAC AAGGGCCGAA 
CTGGCCTACA CGCTGGAGCC TGCAGGCGAG GGCCCTGGGG GCTGTGGCCT TGGCACAGAG 
GAGGAACCCA GGGAGCAGGC TCTCCCAGTG GGTGGTGTGG AGCTGCACCC TGAGGACCCC 
CCACAGTACC AGGAGGTACC TCTGGATGAA GCAGTCACTT TGCGTGCACC TGATGTGCCA 
ATGAGGCCTG GCCAGCTCTT CACTGCCACC CTTCTGCTTC GACACAACTT CACTGCGAGC 
CTTCTTACTC TGCGGATTAA GGTGAAGAAA GGGCTGCAGG TGATAGCTGC CCGCCCAGCC 
CAGCCCACCC TCTGGACTGC TAAGTTAGAC CGCTTCAAGG GCTCAAAGCA CCACACCAGC 
CTGATCACCT GTCACCGTGC TGGGCCTGCA GGGCCAGATT CCAGCCCCCT TGAACTGTCC 
GAGTTCCTGT GGGTGGACTT TGCAGTGGAG AACAGCACTG GTGGGGGTGT GGCAGTCACT 
CGCCCTGTCA CGTGGCAGCT GGAGTACCCA GGTCAGGCCC CCGAAGCAGA GAAGGACAAA 
ATGGTGTGGG AGATCCTGGT GTCTGAGCGG GACATCAGAG CCCTCATCCC TCTGGCCAAG 
GCTGAGGAGC TGGTGAACAC GGCTCCATTG ACTGGAGTGC CCCAGCGTAT TCCGGTGCGG 
CTCGTCACTG TGGACAGTGG GGGAGCCTTG GAGGAGGTGA CAGAGCACAT TGGCTGTGAG 
TCAGCCAATA CACAGGTCCT GCAGGTATCT GAGGCCTGTG ATGCTGTGTT CGTGGCTGGC 
CAGGAGAGCC GGGGTGCCAA GGGGGTGAGG GTGGACTTCT GGTGGCGCCG ACTTCGGGCC 
TCACTAAAGC TGACTGTATG GGCCCCACTC CTGCCCTTGC GCATTGAGCT GACTGACACC 
ACTCTGGAAC AAATCCGAGG CTGGAGGGTC CCAGGCTCAG CAGAAGGGCA GCTGGAGCCT 
GAGACTGCTG CTGAGGAGGT GGAGCGGCGC TCCCGTGGCT GCCGCCTGCA GTACCAGAGA 
GCTGGTGTGC GCTTCTTGGT GCCCTTTGCG GCTCACCCGC TGGACGGTGG CCGTCGCCTC 
ACCCACCTAC TCGGTCCTGA CTGGTTGCTG GATGTGAGTC ACCTCGTGGC AGCCCATGCC 
CATGTGCAAG ATCCTCGCAT AGCCTCCTTG GAAGGTGGCC GCATCCTAGT GGGACGGGAG 
CCTGGAGTCA CCTCTATCGA GGTTCGTTCC CCGCTGTCTG ACGCCATCCT GGGGGAGCAG 
GCACTGGCTG TGACAGACGA CAAGGTCTCA GTGCTGGATC TGCGAGTACA GCCGGTGATG 
GGCATCTCAC TGTCCTTGAG CCGGGGCATG TCCCACCCTG GAGAAGTCAC AGCCACATGC 
TGGGCCCAGT CAGCTCTTCC AGCCCCAAAG CAGGAGGTGG CCCTCTCCCT GTGGCTGTCC 
TTCTCTGACC ACACTTTGGC CCCAGCCGAG CTCTACGACC GCAATGACCT GGGGCTGTCG 
GTCTCAGCTG AGGAGCCCAG TGCTGTTCTG CCAGCCGAAG AGCAGGGAGC CCAGCTTGGT 
GTGGTGGTGA GTGGTGTGGG TGCCGAAGGG CTGCCCCTAC ACGTGGCTCT GCACCCACCC 
GAACCCTGCC GCCGGGGCCG CCATCGCGTT CCCCTGGCAT CAGGCACCGC CTGGCTGGGG 
TTGCCCCCTT TGCCCACACC AGTTCCTGCC CTCCCATCCA GCCCTGTCCG GACCTCACCG 
TTCACAGAAG CCACGGTGGA GGGAAAGCGG CAGATAGCAG GTGATATGGG GGGCCATGTG 
GGTATTAGGG GCAAGTTTGA GCGGGCAGAG GAAGAAGCTG GGAAGGAAGA AAATGAGGCC 
AAAGAAGAGG AGGAAGATGA GGAAGAGATG GTGCCTGCCC CTCAGCGTGT CACCGACCTA 
GAGCTGGGGA TGTATGCTCT TCTGGGCATC TTCTGTTTGG CCATCCTCAT CTTCCTGGTC 
AATGGTGTGG TCTTTGTGTT GCGCTACCAG CGCAAAGAGC CTCCAGACAG CGCTACAGAC 
CCTGCCTCCC CTCAGCCACA TAACTGGGTC TGGCTGGGCA CCAATCAGGA GGAACTGAGT 
CGGCAGCTGG ACCGATGCTC TTCCTCGGGT CCACCCAAGG GGGAGGGGGG CTGCCCTTGT 
GAAAGTGGGG CAGGAGGGGA TGCCTCAACC GTGGCCCCCA GTGCTTCAGA GAGCCCTGCT 
GGCTCCTCGA GCACCCTGGC CCGCAAAGAA GCCGGGGGAC GTCGGAAGCG AGTCGAGTTT 
GTGACATTTG CACCAGCACC CCCAACCCAG CCGCCTGAGG AGCCTGTAGG GGCCCCCGCT 
GTACAGTCCA TCCTGGTGGC AGGTGAGGAG GACATCCGCT GGGTGTGCGA GGACATGGGG 
CTGAAGGACC CAGAGGAGCT TCGAAACTAC ATGGAGAGGA TCCGGGGCAG CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmem132a ( XM_006527503.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527566.1 Mus musculus transmembrane protein 132A (Tmem132a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55840
Clone ID Related Accession (Same CDS sequence)	XM_006527503.1 , XM_006527503.2
Accession Version	XM_006527503.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	transmembrane protein 132A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527503.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAC GCAAGGCTGC GGCCCCTCGG GGGCCCTACG GTGCCTGGTT CTGCCTCCTG 
GTGGCTCTCG CCCTGGAAGT CGTGAGAGTT AGCAGTAACC ATGATACCCT GGACCCCATC 
TACCTGCCAG TGGCCCTGGA ACTCCTAGAT GCCCCTGAGC ATTTTCGTGT GCAGCAGGTG 
GGCCATTACC CACCTGCCAA CTCTTCCCTG GCCTCCCGCT CTGAGACTTT CCTGCTCATG 
CAGCCCTGGC CCAGGGCACA GCCGCTTCTC CGGGCCTCCT ACCCACCCTT TGCCACTCAG 
CAGGTGGTCC CTCCTAGGGT CACCGAGCCC CACCGGCGGC CTGTCCCTTG GGATGTGCGG 
GCTGTTTCCG TGGAAGCAGC GGTGACTCCA GCAGAACCCT ATGCCCGTGT CCTCTTCCAC 
CTCAAAGGGC AGGATTGGCC ACCAGGACCT GGCAGCTTGC CCTGTGCCCG ACTACATGCC 
ACACACCCTG CAGGCACTGC CCACCGAGCC TGCCGCTTCC AGCCATCTCT GGGTGCCTGT 
GTGGTGGAAC TGCAATTCCC CTCTCACTGG TTCTCCCAAA GTGCCACCAC AAGGGCCGAA 
CTGGCCTACA CGCTGGAGCC TGCAGGCGAG GGCCCTGGGG GCTGTGGCCT TGGCACAGAG 
GAGGAACCCA GGGAGCAGGC TCTCCCAGTG GGTGGTGTGG AGCTGCACCC TGAGGACCCC 
CCACAGTACC AGGAGGTACC TCTGGATGAA GCAGTCACTT TGCGTGCACC TGATGTGCCA 
ATGAGGCCTG GCCAGCTCTT CACTGCCACC CTTCTGCTTC GACACAACTT CACTGCGAGC 
CTTCTTACTC TGCGGATTAA GGTGAAGAAA GGGCTGCAGG TGATAGCTGC CCGCCCAGCC 
CAGCCCACCC TCTGGACTGC TAAGTTAGAC CGCTTCAAGG GCTCAAAGCA CCACACCAGC 
CTGATCACCT GTCACCGTGC TGGGCCTGCA GGGCCAGATT CCAGCAGCCC CCTTGAACTG 
TCCGAGTTCC TGTGGGTGGA CTTTGCAGTG GAGAACAGCA CTGGTGGGGG TGTGGCAGTC 
ACTCGCCCTG TCACGTGGCA GCTGGAGTAC CCAGGTCAGG CCCCCGAAGC AGAGAAGGAC 
AAAATGGTGT GGGAGATCCT GGTGTCTGAG CGGGACATCA GAGCCCTCAT CCCTCTGGCC 
AAGGCTGAGG AGCTGGTGAA CACGGCTCCA TTGACTGGAG TGCCCCAGCG TATTCCGGTG 
CGGCTCGTCA CTGTGGACAG TGGGGGAGCC TTGGAGGAGG TGACAGAGCA CATTGGCTGT 
GAGTCAGCCA ATACACAGGT CCTGCAGGTA TCTGAGGCCT GTGATGCTGT GTTCGTGGCT 
GGCCAGGAGA GCCGGGGTGC CAAGGGGGTG AGGGTGGACT TCTGGTGGCG CCGACTTCGG 
GCCTCACTAA AGCTGACTGT ATGGGCCCCA CTCCTGCCCT TGCGCATTGA GCTGACTGAC 
ACCACTCTGG AACAAATCCG AGGCTGGAGG GTCCCAGGCT CAGCAGAAGG GCAGCTGGAG 
CCTGAGACTG CTGCTGAGGA GGTGGAGCGG CGCTCCCGTG GCTGCCGCCT GCAGTACCAG 
AGAGCTGGTG TGCGCTTCTT GGTGCCCTTT GCGGCTCACC CGCTGGACGG TGGCCGTCGC 
CTCACCCACC TACTCGGTCC TGACTGGTTG CTGGATGTGA GTCACCTCGT GGCAGCCCAT 
GCCCATGTGC AAGATCCTCG CATAGCCTCC TTGGAAGGTG GCCGCATCCT AGTGGGACGG 
GAGCCTGGAG TCACCTCTAT CGAGGTTCGT TCCCCGCTGT CTGACGCCAT CCTGGGGGAG 
CAGGCACTGG CTGTGACAGA CGACAAGGTC TCAGTGCTGG ATCTGCGAGT ACAGCCGGTG 
ATGGGCATCT CACTGTCCTT GAGCCGGGGC ATGTCCCACC CTGGAGAAGT CACAGCCACA 
TGCTGGGCCC AGTCAGCTCT TCCAGCCCCA AAGCAGGAGG TGGCCCTCTC CCTGTGGCTG 
TCCTTCTCTG ACCACACTTT GGCCCCAGCC GAGCTCTACG ACCGCAATGA CCTGGGGCTG 
TCGGTCTCAG CTGAGGAGCC CAGTGCTGTT CTGCCAGCCG AAGAGCAGGG AGCCCAGCTT 
GGTGTGGTGG TGAGTGGTGT GGGTGCCGAA GGGCTGCCCC TACACGTGGC TCTGCACCCA 
CCCGAACCCT GCCGCCGGGG CCGCCATCGC GTTCCCCTGG CATCAGGCAC CGCCTGGCTG 
GGGTTGCCCC CTTTGCCCAC ACCAGTTCCT GCCCTCCCAT CCAGCCCTGT CCGGACCTCA 
CCGTTCACAG AAGCCACGGT GGAGGGAAAG CGGCAGATAG CAGGTGATAT GGGGGGCCAT 
GTGGGTATTA GGGGCAAGTT TGAGCGGGCA GAGGAAGAAG CTGGGAAGGA AGAAAATGAG 
GCCAAAGAAG AGGAGGAAGA TGAGGAAGAG ATGGTGCCTG CCCCTCAGCG TGTCACCGAC 
CTAGAGCTGG GGATGTATGC TCTTCTGGGC ATCTTCTGTT TGGCCATCCT CATCTTCCTG 
GTCAATGGTG TGGTCTTTGT GTTGCGCTAC CAGCGCAAAG AGCCTCCAGA CAGCGCTACA 
GACCCTGCCT CCCCTCAGCC ACATAACTGG GTCTGGCTGG GCACCAATCA GGAGGAACTG 
AGTCGGCAGC TGGACCGATG CTCTTCCTCG GGTCCACCCA AGGGGGAGGG GGGCTGCCCT 
TGTGAAAGTG GGGCAGGAGG GGATGCCTCA ACCGTGGCCC CCAGTGCTTC AGAGAGCCCT 
GCTGGCTCCT CGAGCACCCT GGCCCGCAAA GAAGCCGGGG GACGTCGGAA GCGAGTCGAG 
TTTGTGACAT TTGCACCAGC ACCCCCAACC CAGCCGCCTG AGGAGCCTGT AGGGGCCCCC 
GCTGTACAGT CCATCCTGGT GGCAGGTGAG GAGGACATCC GCTGGGTGTG CGAGGACATG 
GGGCTGAAGG ACCCAGAGGA GCTTCGAAAC TACATGGAGA GGATCCGGGG CAGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527566.1
CDS	573..3632
Translation	MTERKAAAPRGPYGAWFCLLVALALEVVRVSSNHDTLDPIYLPVALELLDAPEHFRVQQVGHYPPANSSLASRSETFLLMQPWPRAQPLLRASYPPFATQQVVPPRVTEPHRRPVPWDVRAVSVEAAVTPAEPYARVLFHLKGQDWPPGPGSLPCARLHATHPAGTAHRACRFQPSLGACVVELQFPSHWFSQSATTRAELAYTLEPAGEGPGGCGLGTEEEPREQALPVGGVELHPEDPPQYQEVPLDEAVTLRAPDVPMRPGQLFTATLLLRHNFTASLLTLRIKVKKGLQVIAARPAQPTLWTAKLDRFKGSKHHTSLITCHRAGPAGPDSSSPLELSEFLWVDFAVENSTGGGVAVTRPVTWQLEYPGQAPEAEKDKMVWEILVSERDIRALIPLAKAEELVNTAPLTGVPQRIPVRLVTVDSGGALEEVTEHIGCESANTQVLQVSEACDAVFVAGQESRGAKGVRVDFWWRRLRASLKLTVWAPLLPLRIELTDTTLEQIRGWRVPGSAEGQLEPETAAEEVERRSRGCRLQYQRAGVRFLVPFAAHPLDGGRRLTHLLGPDWLLDVSHLVAAHAHVQDPRIASLEGGRILVGREPGVTSIEVRSPLSDAILGEQALAVTDDKVSVLDLRVQPVMGISLSLSRGMSHPGEVTATCWAQSALPAPKQEVALSLWLSFSDHTLAPAELYDRNDLGLSVSAEEPSAVLPAEEQGAQLGVVVSGVGAEGLPLHVALHPPEPCRRGRHRVPLASGTAWLGLPPLPTPVPALPSSPVRTSPFTEATVEGKRQIAGDMGGHVGIRGKFERAEEEAGKEENEAKEEEEDEEEMVPAPQRVTDLELGMYALLGIFCLAILIFLVNGVVFVLRYQRKEPPDSATDPASPQPHNWVWLGTNQEELSRQLDRCSSSGPPKGEGGCPCESGAGGDASTVAPSASESPAGSSSTLARKEAGGRRKRVEFVTFAPAPPTQPPEEPVGAPAVQSILVAGEEDIRWVCEDMGLKDPEELRNYMERIRGSS

Target ORF information:

RefSeq Version	XM_006527503.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein 132A (Tmem132a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527503.2

ORF Insert Sequence:

ATGACTGAAC GCAAGGCTGC GGCCCCTCGG GGGCCCTACG GTGCCTGGTT CTGCCTCCTG 
GTGGCTCTCG CCCTGGAAGT CGTGAGAGTT AGCAGTAACC ATGATACCCT GGACCCCATC 
TACCTGCCAG TGGCCCTGGA ACTCCTAGAT GCCCCTGAGC ATTTTCGTGT GCAGCAGGTG 
GGCCATTACC CACCTGCCAA CTCTTCCCTG GCCTCCCGCT CTGAGACTTT CCTGCTCATG 
CAGCCCTGGC CCAGGGCACA GCCGCTTCTC CGGGCCTCCT ACCCACCCTT TGCCACTCAG 
CAGGTGGTCC CTCCTAGGGT CACCGAGCCC CACCGGCGGC CTGTCCCTTG GGATGTGCGG 
GCTGTTTCCG TGGAAGCAGC GGTGACTCCA GCAGAACCCT ATGCCCGTGT CCTCTTCCAC 
CTCAAAGGGC AGGATTGGCC ACCAGGACCT GGCAGCTTGC CCTGTGCCCG ACTACATGCC 
ACACACCCTG CAGGCACTGC CCACCGAGCC TGCCGCTTCC AGCCATCTCT GGGTGCCTGT 
GTGGTGGAAC TGCAATTCCC CTCTCACTGG TTCTCCCAAA GTGCCACCAC AAGGGCCGAA 
CTGGCCTACA CGCTGGAGCC TGCAGGCGAG GGCCCTGGGG GCTGTGGCCT TGGCACAGAG 
GAGGAACCCA GGGAGCAGGC TCTCCCAGTG GGTGGTGTGG AGCTGCACCC TGAGGACCCC 
CCACAGTACC AGGAGGTACC TCTGGATGAA GCAGTCACTT TGCGTGCACC TGATGTGCCA 
ATGAGGCCTG GCCAGCTCTT CACTGCCACC CTTCTGCTTC GACACAACTT CACTGCGAGC 
CTTCTTACTC TGCGGATTAA GGTGAAGAAA GGGCTGCAGG TGATAGCTGC CCGCCCAGCC 
CAGCCCACCC TCTGGACTGC TAAGTTAGAC CGCTTCAAGG GCTCAAAGCA CCACACCAGC 
CTGATCACCT GTCACCGTGC TGGGCCTGCA GGGCCAGATT CCAGCAGCCC CCTTGAACTG 
TCCGAGTTCC TGTGGGTGGA CTTTGCAGTG GAGAACAGCA CTGGTGGGGG TGTGGCAGTC 
ACTCGCCCTG TCACGTGGCA GCTGGAGTAC CCAGGTCAGG CCCCCGAAGC AGAGAAGGAC 
AAAATGGTGT GGGAGATCCT GGTGTCTGAG CGGGACATCA GAGCCCTCAT CCCTCTGGCC 
AAGGCTGAGG AGCTGGTGAA CACGGCTCCA TTGACTGGAG TGCCCCAGCG TATTCCGGTG 
CGGCTCGTCA CTGTGGACAG TGGGGGAGCC TTGGAGGAGG TGACAGAGCA CATTGGCTGT 
GAGTCAGCCA ATACACAGGT CCTGCAGGTA TCTGAGGCCT GTGATGCTGT GTTCGTGGCT 
GGCCAGGAGA GCCGGGGTGC CAAGGGGGTG AGGGTGGACT TCTGGTGGCG CCGACTTCGG 
GCCTCACTAA AGCTGACTGT ATGGGCCCCA CTCCTGCCCT TGCGCATTGA GCTGACTGAC 
ACCACTCTGG AACAAATCCG AGGCTGGAGG GTCCCAGGCT CAGCAGAAGG GCAGCTGGAG 
CCTGAGACTG CTGCTGAGGA GGTGGAGCGG CGCTCCCGTG GCTGCCGCCT GCAGTACCAG 
AGAGCTGGTG TGCGCTTCTT GGTGCCCTTT GCGGCTCACC CGCTGGACGG TGGCCGTCGC 
CTCACCCACC TACTCGGTCC TGACTGGTTG CTGGATGTGA GTCACCTCGT GGCAGCCCAT 
GCCCATGTGC AAGATCCTCG CATAGCCTCC TTGGAAGGTG GCCGCATCCT AGTGGGACGG 
GAGCCTGGAG TCACCTCTAT CGAGGTTCGT TCCCCGCTGT CTGACGCCAT CCTGGGGGAG 
CAGGCACTGG CTGTGACAGA CGACAAGGTC TCAGTGCTGG ATCTGCGAGT ACAGCCGGTG 
ATGGGCATCT CACTGTCCTT GAGCCGGGGC ATGTCCCACC CTGGAGAAGT CACAGCCACA 
TGCTGGGCCC AGTCAGCTCT TCCAGCCCCA AAGCAGGAGG TGGCCCTCTC CCTGTGGCTG 
TCCTTCTCTG ACCACACTTT GGCCCCAGCC GAGCTCTACG ACCGCAATGA CCTGGGGCTG 
TCGGTCTCAG CTGAGGAGCC CAGTGCTGTT CTGCCAGCCG AAGAGCAGGG AGCCCAGCTT 
GGTGTGGTGG TGAGTGGTGT GGGTGCCGAA GGGCTGCCCC TACACGTGGC TCTGCACCCA 
CCCGAACCCT GCCGCCGGGG CCGCCATCGC GTTCCCCTGG CATCAGGCAC CGCCTGGCTG 
GGGTTGCCCC CTTTGCCCAC ACCAGTTCCT GCCCTCCCAT CCAGCCCTGT CCGGACCTCA 
CCGTTCACAG AAGCCACGGT GGAGGGAAAG CGGCAGATAG CAGGTGATAT GGGGGGCCAT 
GTGGGTATTA GGGGCAAGTT TGAGCGGGCA GAGGAAGAAG CTGGGAAGGA AGAAAATGAG 
GCCAAAGAAG AGGAGGAAGA TGAGGAAGAG ATGGTGCCTG CCCCTCAGCG TGTCACCGAC 
CTAGAGCTGG GGATGTATGC TCTTCTGGGC ATCTTCTGTT TGGCCATCCT CATCTTCCTG 
GTCAATGGTG TGGTCTTTGT GTTGCGCTAC CAGCGCAAAG AGCCTCCAGA CAGCGCTACA 
GACCCTGCCT CCCCTCAGCC ACATAACTGG GTCTGGCTGG GCACCAATCA GGAGGAACTG 
AGTCGGCAGC TGGACCGATG CTCTTCCTCG GGTCCACCCA AGGGGGAGGG GGGCTGCCCT 
TGTGAAAGTG GGGCAGGAGG GGATGCCTCA ACCGTGGCCC CCAGTGCTTC AGAGAGCCCT 
GCTGGCTCCT CGAGCACCCT GGCCCGCAAA GAAGCCGGGG GACGTCGGAA GCGAGTCGAG 
TTTGTGACAT TTGCACCAGC ACCCCCAACC CAGCCGCCTG AGGAGCCTGT AGGGGCCCCC 
GCTGTACAGT CCATCCTGGT GGCAGGTGAG GAGGACATCC GCTGGGTGTG CGAGGACATG 
GGGCTGAAGG ACCCAGAGGA GCTTCGAAAC TACATGGAGA GGATCCGGGG CAGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmem132a ( XM_006527503.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527566.1 Mus musculus transmembrane protein 132A (Tmem132a), transcript variant X1, mRNA.

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CloneID	OMu55840
Clone ID Related Accession (Same CDS sequence)	XM_006527503.1 , XM_006527503.2
Accession Version	XM_006527503.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transmembrane protein 132A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAC GCAAGGCTGC GGCCCCTCGG GGGCCCTACG GTGCCTGGTT CTGCCTCCTG 
GTGGCTCTCG CCCTGGAAGT CGTGAGAGTT AGCAGTAACC ATGATACCCT GGACCCCATC 
TACCTGCCAG TGGCCCTGGA ACTCCTAGAT GCCCCTGAGC ATTTTCGTGT GCAGCAGGTG 
GGCCATTACC CACCTGCCAA CTCTTCCCTG GCCTCCCGCT CTGAGACTTT CCTGCTCATG 
CAGCCCTGGC CCAGGGCACA GCCGCTTCTC CGGGCCTCCT ACCCACCCTT TGCCACTCAG 
CAGGTGGTCC CTCCTAGGGT CACCGAGCCC CACCGGCGGC CTGTCCCTTG GGATGTGCGG 
GCTGTTTCCG TGGAAGCAGC GGTGACTCCA GCAGAACCCT ATGCCCGTGT CCTCTTCCAC 
CTCAAAGGGC AGGATTGGCC ACCAGGACCT GGCAGCTTGC CCTGTGCCCG ACTACATGCC 
ACACACCCTG CAGGCACTGC CCACCGAGCC TGCCGCTTCC AGCCATCTCT GGGTGCCTGT 
GTGGTGGAAC TGCAATTCCC CTCTCACTGG TTCTCCCAAA GTGCCACCAC AAGGGCCGAA 
CTGGCCTACA CGCTGGAGCC TGCAGGCGAG GGCCCTGGGG GCTGTGGCCT TGGCACAGAG 
GAGGAACCCA GGGAGCAGGC TCTCCCAGTG GGTGGTGTGG AGCTGCACCC TGAGGACCCC 
CCACAGTACC AGGAGGTACC TCTGGATGAA GCAGTCACTT TGCGTGCACC TGATGTGCCA 
ATGAGGCCTG GCCAGCTCTT CACTGCCACC CTTCTGCTTC GACACAACTT CACTGCGAGC 
CTTCTTACTC TGCGGATTAA GGTGAAGAAA GGGCTGCAGG TGATAGCTGC CCGCCCAGCC 
CAGCCCACCC TCTGGACTGC TAAGTTAGAC CGCTTCAAGG GCTCAAAGCA CCACACCAGC 
CTGATCACCT GTCACCGTGC TGGGCCTGCA GGGCCAGATT CCAGCAGCCC CCTTGAACTG 
TCCGAGTTCC TGTGGGTGGA CTTTGCAGTG GAGAACAGCA CTGGTGGGGG TGTGGCAGTC 
ACTCGCCCTG TCACGTGGCA GCTGGAGTAC CCAGGTCAGG CCCCCGAAGC AGAGAAGGAC 
AAAATGGTGT GGGAGATCCT GGTGTCTGAG CGGGACATCA GAGCCCTCAT CCCTCTGGCC 
AAGGCTGAGG AGCTGGTGAA CACGGCTCCA TTGACTGGAG TGCCCCAGCG TATTCCGGTG 
CGGCTCGTCA CTGTGGACAG TGGGGGAGCC TTGGAGGAGG TGACAGAGCA CATTGGCTGT 
GAGTCAGCCA ATACACAGGT CCTGCAGGTA TCTGAGGCCT GTGATGCTGT GTTCGTGGCT 
GGCCAGGAGA GCCGGGGTGC CAAGGGGGTG AGGGTGGACT TCTGGTGGCG CCGACTTCGG 
GCCTCACTAA AGCTGACTGT ATGGGCCCCA CTCCTGCCCT TGCGCATTGA GCTGACTGAC 
ACCACTCTGG AACAAATCCG AGGCTGGAGG GTCCCAGGCT CAGCAGAAGG GCAGCTGGAG 
CCTGAGACTG CTGCTGAGGA GGTGGAGCGG CGCTCCCGTG GCTGCCGCCT GCAGTACCAG 
AGAGCTGGTG TGCGCTTCTT GGTGCCCTTT GCGGCTCACC CGCTGGACGG TGGCCGTCGC 
CTCACCCACC TACTCGGTCC TGACTGGTTG CTGGATGTGA GTCACCTCGT GGCAGCCCAT 
GCCCATGTGC AAGATCCTCG CATAGCCTCC TTGGAAGGTG GCCGCATCCT AGTGGGACGG 
GAGCCTGGAG TCACCTCTAT CGAGGTTCGT TCCCCGCTGT CTGACGCCAT CCTGGGGGAG 
CAGGCACTGG CTGTGACAGA CGACAAGGTC TCAGTGCTGG ATCTGCGAGT ACAGCCGGTG 
ATGGGCATCT CACTGTCCTT GAGCCGGGGC ATGTCCCACC CTGGAGAAGT CACAGCCACA 
TGCTGGGCCC AGTCAGCTCT TCCAGCCCCA AAGCAGGAGG TGGCCCTCTC CCTGTGGCTG 
TCCTTCTCTG ACCACACTTT GGCCCCAGCC GAGCTCTACG ACCGCAATGA CCTGGGGCTG 
TCGGTCTCAG CTGAGGAGCC CAGTGCTGTT CTGCCAGCCG AAGAGCAGGG AGCCCAGCTT 
GGTGTGGTGG TGAGTGGTGT GGGTGCCGAA GGGCTGCCCC TACACGTGGC TCTGCACCCA 
CCCGAACCCT GCCGCCGGGG CCGCCATCGC GTTCCCCTGG CATCAGGCAC CGCCTGGCTG 
GGGTTGCCCC CTTTGCCCAC ACCAGTTCCT GCCCTCCCAT CCAGCCCTGT CCGGACCTCA 
CCGTTCACAG AAGCCACGGT GGAGGGAAAG CGGCAGATAG CAGGTGATAT GGGGGGCCAT 
GTGGGTATTA GGGGCAAGTT TGAGCGGGCA GAGGAAGAAG CTGGGAAGGA AGAAAATGAG 
GCCAAAGAAG AGGAGGAAGA TGAGGAAGAG ATGGTGCCTG CCCCTCAGCG TGTCACCGAC 
CTAGAGCTGG GGATGTATGC TCTTCTGGGC ATCTTCTGTT TGGCCATCCT CATCTTCCTG 
GTCAATGGTG TGGTCTTTGT GTTGCGCTAC CAGCGCAAAG AGCCTCCAGA CAGCGCTACA 
GACCCTGCCT CCCCTCAGCC ACATAACTGG GTCTGGCTGG GCACCAATCA GGAGGAACTG 
AGTCGGCAGC TGGACCGATG CTCTTCCTCG GGTCCACCCA AGGGGGAGGG GGGCTGCCCT 
TGTGAAAGTG GGGCAGGAGG GGATGCCTCA ACCGTGGCCC CCAGTGCTTC AGAGAGCCCT 
GCTGGCTCCT CGAGCACCCT GGCCCGCAAA GAAGCCGGGG GACGTCGGAA GCGAGTCGAG 
TTTGTGACAT TTGCACCAGC ACCCCCAACC CAGCCGCCTG AGGAGCCTGT AGGGGCCCCC 
GCTGTACAGT CCATCCTGGT GGCAGGTGAG GAGGACATCC GCTGGGTGTG CGAGGACATG 
GGGCTGAAGG ACCCAGAGGA GCTTCGAAAC TACATGGAGA GGATCCGGGG CAGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527566.1
CDS	310..3369
Translation	MTERKAAAPRGPYGAWFCLLVALALEVVRVSSNHDTLDPIYLPVALELLDAPEHFRVQQVGHYPPANSSLASRSETFLLMQPWPRAQPLLRASYPPFATQQVVPPRVTEPHRRPVPWDVRAVSVEAAVTPAEPYARVLFHLKGQDWPPGPGSLPCARLHATHPAGTAHRACRFQPSLGACVVELQFPSHWFSQSATTRAELAYTLEPAGEGPGGCGLGTEEEPREQALPVGGVELHPEDPPQYQEVPLDEAVTLRAPDVPMRPGQLFTATLLLRHNFTASLLTLRIKVKKGLQVIAARPAQPTLWTAKLDRFKGSKHHTSLITCHRAGPAGPDSSSPLELSEFLWVDFAVENSTGGGVAVTRPVTWQLEYPGQAPEAEKDKMVWEILVSERDIRALIPLAKAEELVNTAPLTGVPQRIPVRLVTVDSGGALEEVTEHIGCESANTQVLQVSEACDAVFVAGQESRGAKGVRVDFWWRRLRASLKLTVWAPLLPLRIELTDTTLEQIRGWRVPGSAEGQLEPETAAEEVERRSRGCRLQYQRAGVRFLVPFAAHPLDGGRRLTHLLGPDWLLDVSHLVAAHAHVQDPRIASLEGGRILVGREPGVTSIEVRSPLSDAILGEQALAVTDDKVSVLDLRVQPVMGISLSLSRGMSHPGEVTATCWAQSALPAPKQEVALSLWLSFSDHTLAPAELYDRNDLGLSVSAEEPSAVLPAEEQGAQLGVVVSGVGAEGLPLHVALHPPEPCRRGRHRVPLASGTAWLGLPPLPTPVPALPSSPVRTSPFTEATVEGKRQIAGDMGGHVGIRGKFERAEEEAGKEENEAKEEEEDEEEMVPAPQRVTDLELGMYALLGIFCLAILIFLVNGVVFVLRYQRKEPPDSATDPASPQPHNWVWLGTNQEELSRQLDRCSSSGPPKGEGGCPCESGAGGDASTVAPSASESPAGSSSTLARKEAGGRRKRVEFVTFAPAPPTQPPEEPVGAPAVQSILVAGEEDIRWVCEDMGLKDPEELRNYMERIRGSS

Target ORF information:

RefSeq Version	XM_006527503.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein 132A (Tmem132a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527503.1

ORF Insert Sequence:

ATGACTGAAC GCAAGGCTGC GGCCCCTCGG GGGCCCTACG GTGCCTGGTT CTGCCTCCTG 
GTGGCTCTCG CCCTGGAAGT CGTGAGAGTT AGCAGTAACC ATGATACCCT GGACCCCATC 
TACCTGCCAG TGGCCCTGGA ACTCCTAGAT GCCCCTGAGC ATTTTCGTGT GCAGCAGGTG 
GGCCATTACC CACCTGCCAA CTCTTCCCTG GCCTCCCGCT CTGAGACTTT CCTGCTCATG 
CAGCCCTGGC CCAGGGCACA GCCGCTTCTC CGGGCCTCCT ACCCACCCTT TGCCACTCAG 
CAGGTGGTCC CTCCTAGGGT CACCGAGCCC CACCGGCGGC CTGTCCCTTG GGATGTGCGG 
GCTGTTTCCG TGGAAGCAGC GGTGACTCCA GCAGAACCCT ATGCCCGTGT CCTCTTCCAC 
CTCAAAGGGC AGGATTGGCC ACCAGGACCT GGCAGCTTGC CCTGTGCCCG ACTACATGCC 
ACACACCCTG CAGGCACTGC CCACCGAGCC TGCCGCTTCC AGCCATCTCT GGGTGCCTGT 
GTGGTGGAAC TGCAATTCCC CTCTCACTGG TTCTCCCAAA GTGCCACCAC AAGGGCCGAA 
CTGGCCTACA CGCTGGAGCC TGCAGGCGAG GGCCCTGGGG GCTGTGGCCT TGGCACAGAG 
GAGGAACCCA GGGAGCAGGC TCTCCCAGTG GGTGGTGTGG AGCTGCACCC TGAGGACCCC 
CCACAGTACC AGGAGGTACC TCTGGATGAA GCAGTCACTT TGCGTGCACC TGATGTGCCA 
ATGAGGCCTG GCCAGCTCTT CACTGCCACC CTTCTGCTTC GACACAACTT CACTGCGAGC 
CTTCTTACTC TGCGGATTAA GGTGAAGAAA GGGCTGCAGG TGATAGCTGC CCGCCCAGCC 
CAGCCCACCC TCTGGACTGC TAAGTTAGAC CGCTTCAAGG GCTCAAAGCA CCACACCAGC 
CTGATCACCT GTCACCGTGC TGGGCCTGCA GGGCCAGATT CCAGCAGCCC CCTTGAACTG 
TCCGAGTTCC TGTGGGTGGA CTTTGCAGTG GAGAACAGCA CTGGTGGGGG TGTGGCAGTC 
ACTCGCCCTG TCACGTGGCA GCTGGAGTAC CCAGGTCAGG CCCCCGAAGC AGAGAAGGAC 
AAAATGGTGT GGGAGATCCT GGTGTCTGAG CGGGACATCA GAGCCCTCAT CCCTCTGGCC 
AAGGCTGAGG AGCTGGTGAA CACGGCTCCA TTGACTGGAG TGCCCCAGCG TATTCCGGTG 
CGGCTCGTCA CTGTGGACAG TGGGGGAGCC TTGGAGGAGG TGACAGAGCA CATTGGCTGT 
GAGTCAGCCA ATACACAGGT CCTGCAGGTA TCTGAGGCCT GTGATGCTGT GTTCGTGGCT 
GGCCAGGAGA GCCGGGGTGC CAAGGGGGTG AGGGTGGACT TCTGGTGGCG CCGACTTCGG 
GCCTCACTAA AGCTGACTGT ATGGGCCCCA CTCCTGCCCT TGCGCATTGA GCTGACTGAC 
ACCACTCTGG AACAAATCCG AGGCTGGAGG GTCCCAGGCT CAGCAGAAGG GCAGCTGGAG 
CCTGAGACTG CTGCTGAGGA GGTGGAGCGG CGCTCCCGTG GCTGCCGCCT GCAGTACCAG 
AGAGCTGGTG TGCGCTTCTT GGTGCCCTTT GCGGCTCACC CGCTGGACGG TGGCCGTCGC 
CTCACCCACC TACTCGGTCC TGACTGGTTG CTGGATGTGA GTCACCTCGT GGCAGCCCAT 
GCCCATGTGC AAGATCCTCG CATAGCCTCC TTGGAAGGTG GCCGCATCCT AGTGGGACGG 
GAGCCTGGAG TCACCTCTAT CGAGGTTCGT TCCCCGCTGT CTGACGCCAT CCTGGGGGAG 
CAGGCACTGG CTGTGACAGA CGACAAGGTC TCAGTGCTGG ATCTGCGAGT ACAGCCGGTG 
ATGGGCATCT CACTGTCCTT GAGCCGGGGC ATGTCCCACC CTGGAGAAGT CACAGCCACA 
TGCTGGGCCC AGTCAGCTCT TCCAGCCCCA AAGCAGGAGG TGGCCCTCTC CCTGTGGCTG 
TCCTTCTCTG ACCACACTTT GGCCCCAGCC GAGCTCTACG ACCGCAATGA CCTGGGGCTG 
TCGGTCTCAG CTGAGGAGCC CAGTGCTGTT CTGCCAGCCG AAGAGCAGGG AGCCCAGCTT 
GGTGTGGTGG TGAGTGGTGT GGGTGCCGAA GGGCTGCCCC TACACGTGGC TCTGCACCCA 
CCCGAACCCT GCCGCCGGGG CCGCCATCGC GTTCCCCTGG CATCAGGCAC CGCCTGGCTG 
GGGTTGCCCC CTTTGCCCAC ACCAGTTCCT GCCCTCCCAT CCAGCCCTGT CCGGACCTCA 
CCGTTCACAG AAGCCACGGT GGAGGGAAAG CGGCAGATAG CAGGTGATAT GGGGGGCCAT 
GTGGGTATTA GGGGCAAGTT TGAGCGGGCA GAGGAAGAAG CTGGGAAGGA AGAAAATGAG 
GCCAAAGAAG AGGAGGAAGA TGAGGAAGAG ATGGTGCCTG CCCCTCAGCG TGTCACCGAC 
CTAGAGCTGG GGATGTATGC TCTTCTGGGC ATCTTCTGTT TGGCCATCCT CATCTTCCTG 
GTCAATGGTG TGGTCTTTGT GTTGCGCTAC CAGCGCAAAG AGCCTCCAGA CAGCGCTACA 
GACCCTGCCT CCCCTCAGCC ACATAACTGG GTCTGGCTGG GCACCAATCA GGAGGAACTG 
AGTCGGCAGC TGGACCGATG CTCTTCCTCG GGTCCACCCA AGGGGGAGGG GGGCTGCCCT 
TGTGAAAGTG GGGCAGGAGG GGATGCCTCA ACCGTGGCCC CCAGTGCTTC AGAGAGCCCT 
GCTGGCTCCT CGAGCACCCT GGCCCGCAAA GAAGCCGGGG GACGTCGGAA GCGAGTCGAG 
TTTGTGACAT TTGCACCAGC ACCCCCAACC CAGCCGCCTG AGGAGCCTGT AGGGGCCCCC 
GCTGTACAGT CCATCCTGGT GGCAGGTGAG GAGGACATCC GCTGGGTGTG CGAGGACATG 
GGGCTGAAGG ACCCAGAGGA GCTTCGAAAC TACATGGAGA GGATCCGGGG CAGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Transcriptional and post-transcriptional regulation of transmembrane protein 132A.
Molecular and cellular biochemistry405(1-2)291-9(2015 Jul)
Oh-Hashi K,Sone A,Hikiji T,Hirata Y,Vitiello M,Fedele M,Ishigaki S,Sobue G,Kiuchi K

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Characterization of the expression and cell-surface localization of transmembrane protein 132A.
Molecular and cellular biochemistry370(1-2)23-33(2012 Nov)
Oh-hashi K,Koga H,Nagase T,Hirata Y,Kiuchi K

Essential role of the N-terminus of murine Orai1 in store-operated Ca2+ entry.
Biochemical and biophysical research communications356(1)45-52(2007 Apr)
Takahashi Y,Murakami M,Watanabe H,Hasegawa H,Ohba T,Munehisa Y,Nobori K,Ono K,Iijima T,Ito H

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Tmem132a cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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