Prg4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prg4
Entrez Gene ID	96875
Full Name	proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein)
Synonyms	CACP,DOL54,JCAP,MSF,SZP,lubricin
General protein information	Preferred Names proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) Names proteoglycan 4 megakaryocyte-stimulating factor proteoglycan 3 superficial zone proteoglycan
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 G1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017313032.1	XP_017168521.1	proteoglycan 4 isoform X1
XM_006529966.1	XP_006530029.1	proteoglycan 4 isoform X2
NM_021400.3	NP_067375.3	proteoglycan 4 isoform 1 precursor
XM_006529963.1	XP_006530026.1	proteoglycan 4 isoform X1
XM_017313032.2	XP_017168521.1	proteoglycan 4 isoform X1
NM_001110146.1	NP_001103616.1	proteoglycan 4 isoform 2 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prg4	NP_067375.3
Canis lupus familiaris (dog)	PRG4	XP_005623018.1

Gene Ontology

The following Prg4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prg4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14193	NM_001110146.1 Latest version!	Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu28504	XM_017313032.2 Latest version!	Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu14224	NM_021400.3 Latest version!	Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$720.30 $1029.00
OMu28504	XM_006529963.1 Latest version!	Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu28506	XM_006529966.1 Latest version!	Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
View Old Accession Versions >>
OMu28504	XM_017313032.1	Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prg4 ( NM_001110146.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001103616.1 Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu14193
Clone ID Related Accession (Same CDS sequence)	NM_001110146.1
Accession Version	NM_001110146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	proteoglycan 4 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK149469.1 and BE457731.1. Transcript Variant: This variant (2) lacks internal coding exons and segments, as compared to variant 1. The resulting isoform (2) is much shorter but has identical N- and C-termini, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK149469.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGTGGA AAATACTTCC CGTCTGCTTG TCGCTGCTGC TTCCTGTTGT CTTGATTCAA 
CAGGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTACTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG TCCTGATTTC 
AAGAGAGTCT GCACTACAGA GCTCTCCTGC AAAGGACGCT GCTTCGAGTC CTTCGCTCGA 
GGGAGGGAGT GTGACTGTGA CTCCCAGTGT AAACAGTATG GCAAGTGCTG TGCAGATTAT 
GACAGCTTCT GTGAAGAAGT AAAAGATAAC AAGAAAAACA CTCCTAAAAA GAAACCCAAT 
CCAGAACCGC CGGCTGTGGA TGAAGCTGGA AGCGGGCTGG ATAATGCTAT TCCAGGCACT 
GATCTTTTGG CCGGGAGACT CAATCAAGGC ATTAACATCA ATCCCATGCT TTCAGATGAG 
ACCAATTTAT GCAATGGTAA GCCAGTGGAT GGACTGACTA CGCTGCGCAA TGGGACATTA 
GTTGCATTTC GAGGTCATTA TTTCTGGATG CTGAATCCAT TCAGACCACC ATCTCCACCA 
CGCAGAATTA CCGAAGTTTG GGGTATTCCC TCTCCTATTG ACACAGTTTT TACTAGATGT 
AACTGTGAAG GAAAAACTTT CTTCTTTAAG GATTCTCAGT ACTGGCGCTT CACCAATGAT 
GTGGTAGATC CTGGGTATCC TAAACAAATT GTCAAAGGCT TTGGAGGACT AACAGGGAAG 
ATAGTGGCTG CTCTTTCAAT AGCCAAGTAC AAGGACAGGC CTGAATCTGT GTACTTCTTC 
AAGAGAGGTG GCAACATTCA GCAGTACACT TATAAACAGG AGCCAATGAA GAAGTGCACA 
GGGAGGCGGC CTGCCATCAA CTACTCGGTG TACGGGGAGG CAGCACAGGT CAGGAGGCGC 
CGCTTTGAGC GTGCTGTTGG ACCTTTCCAG ACACACACCT TCAGGATCCA TTACTCGGTG 
CCCATGAGAG TTTCTTATCA AGACAAAGGT TTCCTCCACA ATGAAGTCAA AGTGAGTACA 
ATGTGGAGAG GGTTTCCAAA TGTTGTTACT TCAGCGATAA CACTGCCTAA CATTAGGAAA 
CCTGATGGCT ATGATTACTA CGCCTTTTCC AAAGATCAAT ACTATAACAT TGATGTGCCT 
ACAAGAACAG CAAGAGCAAT TACCACACGT TCAGGGCAGA CCCTCTCCAA AATCTGGTAC 
AACTGTCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103616.1
CDS	41..1312
Translation	MGWKILPVCLSLLLPVVLIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTTELSCKGRCFESFARGRECDCDSQCKQYGKCCADYDSFCEEVKDNKKNTPKKKPNPEPPAVDEAGSGLDNAIPGTDLLAGRLNQGININPMLSDETNLCNGKPVDGLTTLRNGTLVAFRGHYFWMLNPFRPPSPPRRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDVVDPGYPKQIVKGFGGLTGKIVAALSIAKYKDRPESVYFFKRGGNIQQYTYKQEPMKKCTGRRPAINYSVYGEAAQVRRRRFERAVGPFQTHTFRIHYSVPMRVSYQDKGFLHNEVKVSTMWRGFPNVVTSAITLPNIRKPDGYDYYAFSKDQYYNIDVPTRTARAITTRSGQTLSKIWYNCP

Target ORF information:

RefSeq Version	NM_001110146.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110146.1

ORF Insert Sequence:

ATGGGGTGGA AAATACTTCC CGTCTGCTTG TCGCTGCTGC TTCCTGTTGT CTTGATTCAA 
CAGGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTACTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG TCCTGATTTC 
AAGAGAGTCT GCACTACAGA GCTCTCCTGC AAAGGACGCT GCTTCGAGTC CTTCGCTCGA 
GGGAGGGAGT GTGACTGTGA CTCCCAGTGT AAACAGTATG GCAAGTGCTG TGCAGATTAT 
GACAGCTTCT GTGAAGAAGT AAAAGATAAC AAGAAAAACA CTCCTAAAAA GAAACCCAAT 
CCAGAACCGC CGGCTGTGGA TGAAGCTGGA AGCGGGCTGG ATAATGCTAT TCCAGGCACT 
GATCTTTTGG CCGGGAGACT CAATCAAGGC ATTAACATCA ATCCCATGCT TTCAGATGAG 
ACCAATTTAT GCAATGGTAA GCCAGTGGAT GGACTGACTA CGCTGCGCAA TGGGACATTA 
GTTGCATTTC GAGGTCATTA TTTCTGGATG CTGAATCCAT TCAGACCACC ATCTCCACCA 
CGCAGAATTA CCGAAGTTTG GGGTATTCCC TCTCCTATTG ACACAGTTTT TACTAGATGT 
AACTGTGAAG GAAAAACTTT CTTCTTTAAG GATTCTCAGT ACTGGCGCTT CACCAATGAT 
GTGGTAGATC CTGGGTATCC TAAACAAATT GTCAAAGGCT TTGGAGGACT AACAGGGAAG 
ATAGTGGCTG CTCTTTCAAT AGCCAAGTAC AAGGACAGGC CTGAATCTGT GTACTTCTTC 
AAGAGAGGTG GCAACATTCA GCAGTACACT TATAAACAGG AGCCAATGAA GAAGTGCACA 
GGGAGGCGGC CTGCCATCAA CTACTCGGTG TACGGGGAGG CAGCACAGGT CAGGAGGCGC 
CGCTTTGAGC GTGCTGTTGG ACCTTTCCAG ACACACACCT TCAGGATCCA TTACTCGGTG 
CCCATGAGAG TTTCTTATCA AGACAAAGGT TTCCTCCACA ATGAAGTCAA AGTGAGTACA 
ATGTGGAGAG GGTTTCCAAA TGTTGTTACT TCAGCGATAA CACTGCCTAA CATTAGGAAA 
CCTGATGGCT ATGATTACTA CGCCTTTTCC AAAGATCAAT ACTATAACAT TGATGTGCCT 
ACAAGAACAG CAAGAGCAAT TACCACACGT TCAGGGCAGA CCCTCTCCAA AATCTGGTAC 
AACTGTCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prg4 ( XM_017313032.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168521.1 Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu28504
Clone ID Related Accession (Same CDS sequence)	XM_017313032.1 , XM_006529963.1 , XM_017313032.2
Accession Version	XM_017313032.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	proteoglycan 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017313032.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTGGA AAATACTTCC CGTCTGCTTG TCGCTGCTGC TTCCTGTTGT CTTGATTCAA 
CAGGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTACTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG TCCTGATTTC 
AAGAGAGTCT GCACTACAGA GCTCTCCTGC AAAGGACGCT GCTTCGAGTC CTTCGCTCGA 
GGGAGGGAGT GTGACTGTGA CTCCCAGTGT AAACAGTATG GCAAGTGCTG TGCAGATTAT 
GACAGCTTCT GTGAAGAAGT AAAAGATAAC AAGAAAAACA CTCCTAAAAA GAAACCCAAT 
CCAGAACCGC CGGCTGTGGA TGAAGCTGGA AGCGGGCTGG ATAATGGTGA GTTCAAGCTC 
ACCCCACCTC CTCCTGACCC TCCTACTACT CCACACAGCA AAGTCACCAC ATCTCCCAAG 
ACTACAGCAG CAAAACCAGT AACTCCTAAA CCCAGTCTTG CACCTAATTC TGAGACATCC 
AAAGAGGCAT CTTTAGCATC CAATAAAGAG ACAACAGTTG AAACGAAAGA GACTACTGCA 
ACAAATAAAC AATCTTCTGC CAGTAAAAAA AAGACGACTT CAGCTAAAGA GACACGGAGT 
GCAGAGAAAA CATCTGATAA AGATGTTGAG CCCACATCTA CAACTCCCAA GAACTCAGCT 
CCCACTACTA CCAAGAAGCC TGTTACTACA ACCAAGGAAC CAGTTCCTAC CACTCCCAAG 
GAGCCTGAAC CCACAACTCC CAAGGAACCA CCTCCAACCA CCAAGAAGCC TGAACCCACC 
ACCCCCAAGG AGCCTGAACC CACAACTCCC AAGGAGCCTG AACCCACCAC CCCCAAGGAG 
CCTGAACCCA CCACTCCCAA GGAGCCTGAA CCCACAACTC CCAAGGAACC ACCTCCAACC 
ACCAAGAAGC CTGAACCCAC CACCCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACCA CCACCAAGGA GCCTGAACCC ACCACCACCA AGGAGCCTGA ATCCACCACC 
CGCAAGGAGC CTGAACCCAC CACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACAACTCCCA AGGAGCCTGA ACCCACCACT 
CTCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACAACTCCCA AGGAGCCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACCA CGCCCAAGGA GCCTGAACCC ACAACCCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACCACTCT CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACCACTCCCA AGGAGCCTGA ACCCACCACT 
CTCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACCACTCT CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACCACCACCA AGGAGCCTGA ATCCACCACC 
CGCAAGGAGC CTGAACCCAC CACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGGA GCCTGAACCC ACCACGCCCA AGGAGCCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACAACCCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGAA ACCTGAACCC ACAACTCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC CACCCCCAAG GAGCCTGAAC CCACCACGCC CAAGGAGCCT 
GAACCCACAA CTCGCAAGGA GCCTGAACCT ACAACTCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG AAGCCTGAAC CCACCACCAC ATCACCCAAA 
ACCACTACTC TGAAAGCAAC AACTCTTGCA CCCAAAGTAA CTGCTCCAGC AGAAGAGATT 
CAGAACAAAC CTGAAGAAAC AACTCCTGCA TCAGAAGATT CTGATGATTC TAAAACAACT 
CTAAAACCAC AGAAGCCAAC CAAAGCACCC AAGCCTACCA AAAAGCCAAC CAAAGCACCC 
AAGAAGCCCA CCTCTACCAA AAAGCCAAAG ACACCAAAAA CAAGAAAACC AAAAACTACA 
CCATCTCCTC TAAAGACGAC TTCAGCAACA CCTGAACTGA ATACCACCCC TCTAGAAGTC 
ATGCTGCCAA CCACCACCAT CCCTAAACAA ACTCCAAACC CTGAAACAGC TGAAGTAAAT 
CCAGATCATG AAGATGCAGA TGGAGGTGAA GGAGAAAAAC CTCTGATTCC CGGGCCCCCT 
GTGCTATTCC CCACAGCTAT TCCAGGCACT GATCTTTTGG CCGGGAGACT CAATCAAGGC 
ATTAACATCA ATCCCATGCT TTCAGATGAG ACCAATTTAT GCAATGGTAA GCCAGTGGAT 
GGACTGACTA CGCTGCGCAA TGGGACATTA GTTGCATTTC GAGGTCATTA TTTCTGGATG 
CTGAATCCAT TCAGACCACC ATCTCCACCA CGCAGAATTA CCGAAGTTTG GGGTATTCCC 
TCTCCTATTG ACACAGTTTT TACTAGATGT AACTGTGAAG GAAAAACTTT CTTCTTTAAG 
GATTCTCAGT ACTGGCGCTT CACCAATGAT GTGGTAGATC CTGGGTATCC TAAACAAATT 
GTCAAAGGCT TTGGAGGACT AACAGGGAAG ATAGTGGCTG CTCTTTCAAT AGCCAAGTAC 
AAGGACAGGC CTGAATCTGT GTACTTCTTC AAGAGAGGTG GCAACATTCA GCAGTACACT 
TATAAACAGG AGCCAATGAA GAAGTGCACA GGGAGGCGGC CTGCCATCAA CTACTCGGTG 
TACGGGGAGG CAGCACAGGT CAGGAGGCGC CGCTTTGAGC GTGCTGTTGG ACCTTTCCAG 
ACACACACCT TCAGGATCCA TTACTCGGTG CCCATGAGAG TTTCTTATCA AGACAAAGGT 
TTCCTCCACA ATGAAGTCAA AGTGAGTACA ATGTGGAGAG GGTTTCCAAA TGTTGTTACT 
TCAGCGATAA CACTGCCTAA CATTAGGAAA CCTGATGGCT ATGATTACTA CGCCTTTTCC 
AAAGATCAAT ACTATAACAT TGATGTGCCT ACAAGAACAG CAAGAGCAAT TACCACACGT 
TCAGGGCAGA CCCTCTCCAA AATCTGGTAC AACTGTCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168521.1
CDS	82..3483
Translation	MGWKILPVCLSLLLPVVLIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTTELSCKGRCFESFARGRECDCDSQCKQYGKCCADYDSFCEEVKDNKKNTPKKKPNPEPPAVDEAGSGLDNGEFKLTPPPPDPPTTPHSKVTTSPKTTAAKPVTPKPSLAPNSETSKEASLASNKETTVETKETTATNKQSSASKKKTTSAKETRSAEKTSDKDVEPTSTTPKNSAPTTTKKPVTTTKEPVPTTPKEPEPTTPKEPPPTTKKPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPPPTTKKPEPTTPKEPEPTTPKEPEPTTTKEPEPTTTKEPESTTRKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTTKEPESTTRKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKKPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTRKEPEPTTPKEPEPTTPKEPEPTTPKKPEPTTTSPKTTTLKATTLAPKVTAPAEEIQNKPEETTPASEDSDDSKTTLKPQKPTKAPKPTKKPTKAPKKPTSTKKPKTPKTRKPKTTPSPLKTTSATPELNTTPLEVMLPTTTIPKQTPNPETAEVNPDHEDADGGEGEKPLIPGPPVLFPTAIPGTDLLAGRLNQGININPMLSDETNLCNGKPVDGLTTLRNGTLVAFRGHYFWMLNPFRPPSPPRRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDVVDPGYPKQIVKGFGGLTGKIVAALSIAKYKDRPESVYFFKRGGNIQQYTYKQEPMKKCTGRRPAINYSVYGEAAQVRRRRFERAVGPFQTHTFRIHYSVPMRVSYQDKGFLHNEVKVSTMWRGFPNVVTSAITLPNIRKPDGYDYYAFSKDQYYNIDVPTRTARAITTRSGQTLSKIWYNCP

Target ORF information:

RefSeq Version	XM_017313032.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313032.2

ORF Insert Sequence:

ATGGGGTGGA AAATACTTCC CGTCTGCTTG TCGCTGCTGC TTCCTGTTGT CTTGATTCAA 
CAGGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTACTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG TCCTGATTTC 
AAGAGAGTCT GCACTACAGA GCTCTCCTGC AAAGGACGCT GCTTCGAGTC CTTCGCTCGA 
GGGAGGGAGT GTGACTGTGA CTCCCAGTGT AAACAGTATG GCAAGTGCTG TGCAGATTAT 
GACAGCTTCT GTGAAGAAGT AAAAGATAAC AAGAAAAACA CTCCTAAAAA GAAACCCAAT 
CCAGAACCGC CGGCTGTGGA TGAAGCTGGA AGCGGGCTGG ATAATGGTGA GTTCAAGCTC 
ACCCCACCTC CTCCTGACCC TCCTACTACT CCACACAGCA AAGTCACCAC ATCTCCCAAG 
ACTACAGCAG CAAAACCAGT AACTCCTAAA CCCAGTCTTG CACCTAATTC TGAGACATCC 
AAAGAGGCAT CTTTAGCATC CAATAAAGAG ACAACAGTTG AAACGAAAGA GACTACTGCA 
ACAAATAAAC AATCTTCTGC CAGTAAAAAA AAGACGACTT CAGCTAAAGA GACACGGAGT 
GCAGAGAAAA CATCTGATAA AGATGTTGAG CCCACATCTA CAACTCCCAA GAACTCAGCT 
CCCACTACTA CCAAGAAGCC TGTTACTACA ACCAAGGAAC CAGTTCCTAC CACTCCCAAG 
GAGCCTGAAC CCACAACTCC CAAGGAACCA CCTCCAACCA CCAAGAAGCC TGAACCCACC 
ACCCCCAAGG AGCCTGAACC CACAACTCCC AAGGAGCCTG AACCCACCAC CCCCAAGGAG 
CCTGAACCCA CCACTCCCAA GGAGCCTGAA CCCACAACTC CCAAGGAACC ACCTCCAACC 
ACCAAGAAGC CTGAACCCAC CACCCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACCA CCACCAAGGA GCCTGAACCC ACCACCACCA AGGAGCCTGA ATCCACCACC 
CGCAAGGAGC CTGAACCCAC CACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACAACTCCCA AGGAGCCTGA ACCCACCACT 
CTCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACAACTCCCA AGGAGCCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACCA CGCCCAAGGA GCCTGAACCC ACAACCCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACCACTCT CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACCACTCCCA AGGAGCCTGA ACCCACCACT 
CTCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACCACTCT CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACCACCACCA AGGAGCCTGA ATCCACCACC 
CGCAAGGAGC CTGAACCCAC CACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGGA GCCTGAACCC ACCACGCCCA AGGAGCCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACAACCCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGAA ACCTGAACCC ACAACTCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC CACCCCCAAG GAGCCTGAAC CCACCACGCC CAAGGAGCCT 
GAACCCACAA CTCGCAAGGA GCCTGAACCT ACAACTCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG AAGCCTGAAC CCACCACCAC ATCACCCAAA 
ACCACTACTC TGAAAGCAAC AACTCTTGCA CCCAAAGTAA CTGCTCCAGC AGAAGAGATT 
CAGAACAAAC CTGAAGAAAC AACTCCTGCA TCAGAAGATT CTGATGATTC TAAAACAACT 
CTAAAACCAC AGAAGCCAAC CAAAGCACCC AAGCCTACCA AAAAGCCAAC CAAAGCACCC 
AAGAAGCCCA CCTCTACCAA AAAGCCAAAG ACACCAAAAA CAAGAAAACC AAAAACTACA 
CCATCTCCTC TAAAGACGAC TTCAGCAACA CCTGAACTGA ATACCACCCC TCTAGAAGTC 
ATGCTGCCAA CCACCACCAT CCCTAAACAA ACTCCAAACC CTGAAACAGC TGAAGTAAAT 
CCAGATCATG AAGATGCAGA TGGAGGTGAA GGAGAAAAAC CTCTGATTCC CGGGCCCCCT 
GTGCTATTCC CCACAGCTAT TCCAGGCACT GATCTTTTGG CCGGGAGACT CAATCAAGGC 
ATTAACATCA ATCCCATGCT TTCAGATGAG ACCAATTTAT GCAATGGTAA GCCAGTGGAT 
GGACTGACTA CGCTGCGCAA TGGGACATTA GTTGCATTTC GAGGTCATTA TTTCTGGATG 
CTGAATCCAT TCAGACCACC ATCTCCACCA CGCAGAATTA CCGAAGTTTG GGGTATTCCC 
TCTCCTATTG ACACAGTTTT TACTAGATGT AACTGTGAAG GAAAAACTTT CTTCTTTAAG 
GATTCTCAGT ACTGGCGCTT CACCAATGAT GTGGTAGATC CTGGGTATCC TAAACAAATT 
GTCAAAGGCT TTGGAGGACT AACAGGGAAG ATAGTGGCTG CTCTTTCAAT AGCCAAGTAC 
AAGGACAGGC CTGAATCTGT GTACTTCTTC AAGAGAGGTG GCAACATTCA GCAGTACACT 
TATAAACAGG AGCCAATGAA GAAGTGCACA GGGAGGCGGC CTGCCATCAA CTACTCGGTG 
TACGGGGAGG CAGCACAGGT CAGGAGGCGC CGCTTTGAGC GTGCTGTTGG ACCTTTCCAG 
ACACACACCT TCAGGATCCA TTACTCGGTG CCCATGAGAG TTTCTTATCA AGACAAAGGT 
TTCCTCCACA ATGAAGTCAA AGTGAGTACA ATGTGGAGAG GGTTTCCAAA TGTTGTTACT 
TCAGCGATAA CACTGCCTAA CATTAGGAAA CCTGATGGCT ATGATTACTA CGCCTTTTCC 
AAAGATCAAT ACTATAACAT TGATGTGCCT ACAAGAACAG CAAGAGCAAT TACCACACGT 
TCAGGGCAGA CCCTCTCCAA AATCTGGTAC AACTGTCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prg4 ( NM_021400.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067375.3 Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant 1, mRNA.

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CloneID	OMu14224
Clone ID Related Accession (Same CDS sequence)	NM_021400.3
Accession Version	NM_021400.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Mus musculus(house mouse)
Product	proteoglycan 4 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC161432.5. On Nov 27, 2007 this sequence version replaced NM_021400.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by orthologous human data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGTGGA AAATACTTCC CGTCTGCTTG TCGCTGCTGC TTCCTGTTGT CTTGATTCAA 
CAGGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTACTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG TCCTGATTTC 
AAGAGAGTCT GCACTACAGA GCTCTCCTGC AAAGGACGCT GCTTCGAGTC CTTCGCTCGA 
GGGAGGGAGT GTGACTGTGA CTCCCAGTGT AAACAGTATG GCAAGTGCTG TGCAGATTAT 
GACAGCTTCT GTGAAGAAGT GCATAATCCC ACATCACCAT CTCCAAAGAC TGCACCTCCG 
CCTCCAGGAG CATCTCACAC CATCAAATCA ACTACCAAAC GCTCACCCAA ATCACCAAAA 
AAGAAGACTA AGAAAGTTGT AGAATCTGAG GAAATAACAG AAGAACATTC TGTTTCTGAA 
AATCAAGAGT CCTCCTCCTC CTCCTCTTCT TCCTCTTCAA CTATTCGGAA AATCAAGTCT 
TCCAAAAATT CAGCTAATAG AGAATTACAG AAGAACCCAA AAGTAAAAGA TAACAAGAAA 
AACACTCCTA AAAAGAAACC CAATCCAGAA CCGCCGGCTG TGGATGAAGC TGGAAGCGGG 
CTGGATAATG GTGAGTTCAA GCTCACCCCA CCTCCTCCTG ACCCTCCTAC TACTCCACAC 
AGCAAAGTCA CCACATCTCC CAAGACTACA GCAGCAAAAC CAGTAACTCC TAAACCCAGT 
CTTGCACCTA ATTCTGAGAC ATCCAAAGAG GCATCTTTAG CATCCAATAA AGAGACAACA 
GTTGAAACGA AAGAGACTAC TGCAACAAAT AAACAATCTT CTGCCAGTAA AAAAAAGACG 
ACTTCAGCTA AAGAGACACG GAGTGCAGAG AAAACATCTG ATAAAGATGT TGAGCCCACA 
TCTACAACTC CCAAGAACTC AGCTCCCACT ACTACCAAGA AGCCTGTTAC TACAACCAAG 
GAACCAGTTC CTACCACTCC CAAGGAGCCT GAACCCACAA CTCCCAAGGA ACCACCTCCA 
ACCACCAAGA AGCCTGAACC CACCACCCCC AAGGAGCCTG AACCCACAAC TCCCAAGGAG 
CCTGAACCCA CCACCCCCAA GGAGCCTGAA CCCACCACTC CCAAGGAGCC TGAACCCACA 
ACTCCCAAGG AACCACCTCC AACCACCAAG AAGCCTGAAC CCACCACCCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGGA GCCTGAACCC ACCACCACCA AGGAGCCTGA ACCCACCACC 
ACCAAGGAGC CTGAATCCAC CACCCGCAAG GAGCCTGAAC CCACCACTCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGGA GCCTGAACCT ACAACCCCCA AGGAGCCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCCAC CACTCTCAAG GAGCCTGAAC CCACCACGCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGGA GCCTGAACCT ACAACCCCCA AGGAGCCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGGA GCCTGAACCC ACCACGCCCA AGGAGCCTGA ACCCACAACC 
CCCAAGGAGC CTGAACCTAC AACCCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACCA CTCTCAAGGA GCCTGAACCT ACAACCCCCA AGGAGCCTGA ACCCACCACT 
CCCAAGGAGC CTGAACCCAC CACTCTCAAG GAGCCTGAAC CCACCACGCC CAAGGAGCCT 
GAACCCACCA CTCTCAAGGA GCCTGAACCT ACAACCCCCA AGGAGCCTGA ACCCACCACC 
ACCAAGGAGC CTGAATCCAC CACCCGCAAG GAGCCTGAAC CCACCACTCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGGA GCCTGAACCC ACAACTCCCA AGGAGCCTGA ACCCACCACG 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACCACGCC CAAGGAGCCT 
GAACCCACAA CCCCCAAGGA GCCTGAACCC ACAACTCCCA AGAAACCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCTAC AACCCCCAAG GAGCCTGAAC CCACCACCCC CAAGGAGCCT 
GAACCCACCA CGCCCAAGGA GCCTGAACCC ACAACTCGCA AGGAGCCTGA ACCTACAACT 
CCCAAGGAGC CTGAACCTAC AACCCCCAAG GAGCCTGAAC CCACAACTCC CAAGAAGCCT 
GAACCCACCA CCACATCACC CAAAACCACT ACTCTGAAAG CAACAACTCT TGCACCCAAA 
GTAACTGCTC CAGCAGAAGA GATTCAGAAC AAACCTGAAG AAACAACTCC TGCATCAGAA 
GATTCTGATG ATTCTAAAAC AACTCTAAAA CCACAGAAGC CAACCAAAGC ACCCAAGCCT 
ACCAAAAAGC CAACCAAAGC ACCCAAGAAG CCCACCTCTA CCAAAAAGCC AAAGACACCA 
AAAACAAGAA AACCAAAAAC TACACCATCT CCTCTAAAGA CGACTTCAGC AACACCTGAA 
CTGAATACCA CCCCTCTAGA AGTCATGCTG CCAACCACCA CCATCCCTAA ACAAACTCCA 
AACCCTGAAA CAGCTGAAGT AAATCCAGAT CATGAAGATG CAGATGGAGG TGAAGGAGAA 
AAACCTCTGA TTCCCGGGCC CCCTGTGCTA TTCCCCACAG CTATTCCAGG CACTGATCTT 
TTGGCCGGGA GACTCAATCA AGGCATTAAC ATCAATCCCA TGCTTTCAGA TGAGACCAAT 
TTATGCAATG GTAAGCCAGT GGATGGACTG ACTACGCTGC GCAATGGGAC ATTAGTTGCA 
TTTCGAGGTC ATTATTTCTG GATGCTGAAT CCATTCAGAC CACCATCTCC ACCACGCAGA 
ATTACCGAAG TTTGGGGTAT TCCCTCTCCT ATTGACACAG TTTTTACTAG ATGTAACTGT 
GAAGGAAAAA CTTTCTTCTT TAAGGATTCT CAGTACTGGC GCTTCACCAA TGATGTGGTA 
GATCCTGGGT ATCCTAAACA AATTGTCAAA GGCTTTGGAG GACTAACAGG GAAGATAGTG 
GCTGCTCTTT CAATAGCCAA GTACAAGGAC AGGCCTGAAT CTGTGTACTT CTTCAAGAGA 
GGTGGCAACA TTCAGCAGTA CACTTATAAA CAGGAGCCAA TGAAGAAGTG CACAGGGAGG 
CGGCCTGCCA TCAACTACTC GGTGTACGGG GAGGCAGCAC AGGTCAGGAG GCGCCGCTTT 
GAGCGTGCTG TTGGACCTTT CCAGACACAC ACCTTCAGGA TCCATTACTC GGTGCCCATG 
AGAGTTTCTT ATCAAGACAA AGGTTTCCTC CACAATGAAG TCAAAGTGAG TACAATGTGG 
AGAGGGTTTC CAAATGTTGT TACTTCAGCG ATAACACTGC CTAACATTAG GAAACCTGAT 
GGCTATGATT ACTACGCCTT TTCCAAAGAT CAATACTATA ACATTGATGT GCCTACAAGA 
ACAGCAAGAG CAATTACCAC ACGTTCAGGG CAGACCCTCT CCAAAATCTG GTACAACTGT 
CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067375.3
CDS	41..3706
Translation	MGWKILPVCLSLLLPVVLIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTTELSCKGRCFESFARGRECDCDSQCKQYGKCCADYDSFCEEVHNPTSPSPKTAPPPPGASHTIKSTTKRSPKSPKKKTKKVVESEEITEEHSVSENQESSSSSSSSSSTIRKIKSSKNSANRELQKNPKVKDNKKNTPKKKPNPEPPAVDEAGSGLDNGEFKLTPPPPDPPTTPHSKVTTSPKTTAAKPVTPKPSLAPNSETSKEASLASNKETTVETKETTATNKQSSASKKKTTSAKETRSAEKTSDKDVEPTSTTPKNSAPTTTKKPVTTTKEPVPTTPKEPEPTTPKEPPPTTKKPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPPPTTKKPEPTTPKEPEPTTPKEPEPTTTKEPEPTTTKEPESTTRKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTTKEPESTTRKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKKPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTRKEPEPTTPKEPEPTTPKEPEPTTPKKPEPTTTSPKTTTLKATTLAPKVTAPAEEIQNKPEETTPASEDSDDSKTTLKPQKPTKAPKPTKKPTKAPKKPTSTKKPKTPKTRKPKTTPSPLKTTSATPELNTTPLEVMLPTTTIPKQTPNPETAEVNPDHEDADGGEGEKPLIPGPPVLFPTAIPGTDLLAGRLNQGININPMLSDETNLCNGKPVDGLTTLRNGTLVAFRGHYFWMLNPFRPPSPPRRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDVVDPGYPKQIVKGFGGLTGKIVAALSIAKYKDRPESVYFFKRGGNIQQYTYKQEPMKKCTGRRPAINYSVYGEAAQVRRRRFERAVGPFQTHTFRIHYSVPMRVSYQDKGFLHNEVKVSTMWRGFPNVVTSAITLPNIRKPDGYDYYAFSKDQYYNIDVPTRTARAITTRSGQTLSKIWYNCP

Target ORF information:

RefSeq Version	NM_021400.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021400.3

ORF Insert Sequence:

ATGGGGTGGA AAATACTTCC CGTCTGCTTG TCGCTGCTGC TTCCTGTTGT CTTGATTCAA 
CAGGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTACTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG TCCTGATTTC 
AAGAGAGTCT GCACTACAGA GCTCTCCTGC AAAGGACGCT GCTTCGAGTC CTTCGCTCGA 
GGGAGGGAGT GTGACTGTGA CTCCCAGTGT AAACAGTATG GCAAGTGCTG TGCAGATTAT 
GACAGCTTCT GTGAAGAAGT GCATAATCCC ACATCACCAT CTCCAAAGAC TGCACCTCCG 
CCTCCAGGAG CATCTCACAC CATCAAATCA ACTACCAAAC GCTCACCCAA ATCACCAAAA 
AAGAAGACTA AGAAAGTTGT AGAATCTGAG GAAATAACAG AAGAACATTC TGTTTCTGAA 
AATCAAGAGT CCTCCTCCTC CTCCTCTTCT TCCTCTTCAA CTATTCGGAA AATCAAGTCT 
TCCAAAAATT CAGCTAATAG AGAATTACAG AAGAACCCAA AAGTAAAAGA TAACAAGAAA 
AACACTCCTA AAAAGAAACC CAATCCAGAA CCGCCGGCTG TGGATGAAGC TGGAAGCGGG 
CTGGATAATG GTGAGTTCAA GCTCACCCCA CCTCCTCCTG ACCCTCCTAC TACTCCACAC 
AGCAAAGTCA CCACATCTCC CAAGACTACA GCAGCAAAAC CAGTAACTCC TAAACCCAGT 
CTTGCACCTA ATTCTGAGAC ATCCAAAGAG GCATCTTTAG CATCCAATAA AGAGACAACA 
GTTGAAACGA AAGAGACTAC TGCAACAAAT AAACAATCTT CTGCCAGTAA AAAAAAGACG 
ACTTCAGCTA AAGAGACACG GAGTGCAGAG AAAACATCTG ATAAAGATGT TGAGCCCACA 
TCTACAACTC CCAAGAACTC AGCTCCCACT ACTACCAAGA AGCCTGTTAC TACAACCAAG 
GAACCAGTTC CTACCACTCC CAAGGAGCCT GAACCCACAA CTCCCAAGGA ACCACCTCCA 
ACCACCAAGA AGCCTGAACC CACCACCCCC AAGGAGCCTG AACCCACAAC TCCCAAGGAG 
CCTGAACCCA CCACCCCCAA GGAGCCTGAA CCCACCACTC CCAAGGAGCC TGAACCCACA 
ACTCCCAAGG AACCACCTCC AACCACCAAG AAGCCTGAAC CCACCACCCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGGA GCCTGAACCC ACCACCACCA AGGAGCCTGA ACCCACCACC 
ACCAAGGAGC CTGAATCCAC CACCCGCAAG GAGCCTGAAC CCACCACTCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGGA GCCTGAACCT ACAACCCCCA AGGAGCCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCCAC CACTCTCAAG GAGCCTGAAC CCACCACGCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGGA GCCTGAACCT ACAACCCCCA AGGAGCCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGGA GCCTGAACCC ACCACGCCCA AGGAGCCTGA ACCCACAACC 
CCCAAGGAGC CTGAACCTAC AACCCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACCA CTCTCAAGGA GCCTGAACCT ACAACCCCCA AGGAGCCTGA ACCCACCACT 
CCCAAGGAGC CTGAACCCAC CACTCTCAAG GAGCCTGAAC CCACCACGCC CAAGGAGCCT 
GAACCCACCA CTCTCAAGGA GCCTGAACCT ACAACCCCCA AGGAGCCTGA ACCCACCACC 
ACCAAGGAGC CTGAATCCAC CACCCGCAAG GAGCCTGAAC CCACCACTCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGGA GCCTGAACCC ACAACTCCCA AGGAGCCTGA ACCCACCACG 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACCACGCC CAAGGAGCCT 
GAACCCACAA CCCCCAAGGA GCCTGAACCC ACAACTCCCA AGAAACCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCTAC AACCCCCAAG GAGCCTGAAC CCACCACCCC CAAGGAGCCT 
GAACCCACCA CGCCCAAGGA GCCTGAACCC ACAACTCGCA AGGAGCCTGA ACCTACAACT 
CCCAAGGAGC CTGAACCTAC AACCCCCAAG GAGCCTGAAC CCACAACTCC CAAGAAGCCT 
GAACCCACCA CCACATCACC CAAAACCACT ACTCTGAAAG CAACAACTCT TGCACCCAAA 
GTAACTGCTC CAGCAGAAGA GATTCAGAAC AAACCTGAAG AAACAACTCC TGCATCAGAA 
GATTCTGATG ATTCTAAAAC AACTCTAAAA CCACAGAAGC CAACCAAAGC ACCCAAGCCT 
ACCAAAAAGC CAACCAAAGC ACCCAAGAAG CCCACCTCTA CCAAAAAGCC AAAGACACCA 
AAAACAAGAA AACCAAAAAC TACACCATCT CCTCTAAAGA CGACTTCAGC AACACCTGAA 
CTGAATACCA CCCCTCTAGA AGTCATGCTG CCAACCACCA CCATCCCTAA ACAAACTCCA 
AACCCTGAAA CAGCTGAAGT AAATCCAGAT CATGAAGATG CAGATGGAGG TGAAGGAGAA 
AAACCTCTGA TTCCCGGGCC CCCTGTGCTA TTCCCCACAG CTATTCCAGG CACTGATCTT 
TTGGCCGGGA GACTCAATCA AGGCATTAAC ATCAATCCCA TGCTTTCAGA TGAGACCAAT 
TTATGCAATG GTAAGCCAGT GGATGGACTG ACTACGCTGC GCAATGGGAC ATTAGTTGCA 
TTTCGAGGTC ATTATTTCTG GATGCTGAAT CCATTCAGAC CACCATCTCC ACCACGCAGA 
ATTACCGAAG TTTGGGGTAT TCCCTCTCCT ATTGACACAG TTTTTACTAG ATGTAACTGT 
GAAGGAAAAA CTTTCTTCTT TAAGGATTCT CAGTACTGGC GCTTCACCAA TGATGTGGTA 
GATCCTGGGT ATCCTAAACA AATTGTCAAA GGCTTTGGAG GACTAACAGG GAAGATAGTG 
GCTGCTCTTT CAATAGCCAA GTACAAGGAC AGGCCTGAAT CTGTGTACTT CTTCAAGAGA 
GGTGGCAACA TTCAGCAGTA CACTTATAAA CAGGAGCCAA TGAAGAAGTG CACAGGGAGG 
CGGCCTGCCA TCAACTACTC GGTGTACGGG GAGGCAGCAC AGGTCAGGAG GCGCCGCTTT 
GAGCGTGCTG TTGGACCTTT CCAGACACAC ACCTTCAGGA TCCATTACTC GGTGCCCATG 
AGAGTTTCTT ATCAAGACAA AGGTTTCCTC CACAATGAAG TCAAAGTGAG TACAATGTGG 
AGAGGGTTTC CAAATGTTGT TACTTCAGCG ATAACACTGC CTAACATTAG GAAACCTGAT 
GGCTATGATT ACTACGCCTT TTCCAAAGAT CAATACTATA ACATTGATGT GCCTACAAGA 
ACAGCAAGAG CAATTACCAC ACGTTCAGGG CAGACCCTCT CCAAAATCTG GTACAACTGT 
CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prg4 ( XM_017313032.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168521.1 Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant X2, mRNA.

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CloneID	OMu28504
Clone ID Related Accession (Same CDS sequence)	XM_017313032.1 , XM_006529963.1 , XM_017313032.2
Accession Version	XM_017313032.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	proteoglycan 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTGGA AAATACTTCC CGTCTGCTTG TCGCTGCTGC TTCCTGTTGT CTTGATTCAA 
CAGGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTACTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG TCCTGATTTC 
AAGAGAGTCT GCACTACAGA GCTCTCCTGC AAAGGACGCT GCTTCGAGTC CTTCGCTCGA 
GGGAGGGAGT GTGACTGTGA CTCCCAGTGT AAACAGTATG GCAAGTGCTG TGCAGATTAT 
GACAGCTTCT GTGAAGAAGT AAAAGATAAC AAGAAAAACA CTCCTAAAAA GAAACCCAAT 
CCAGAACCGC CGGCTGTGGA TGAAGCTGGA AGCGGGCTGG ATAATGGTGA GTTCAAGCTC 
ACCCCACCTC CTCCTGACCC TCCTACTACT CCACACAGCA AAGTCACCAC ATCTCCCAAG 
ACTACAGCAG CAAAACCAGT AACTCCTAAA CCCAGTCTTG CACCTAATTC TGAGACATCC 
AAAGAGGCAT CTTTAGCATC CAATAAAGAG ACAACAGTTG AAACGAAAGA GACTACTGCA 
ACAAATAAAC AATCTTCTGC CAGTAAAAAA AAGACGACTT CAGCTAAAGA GACACGGAGT 
GCAGAGAAAA CATCTGATAA AGATGTTGAG CCCACATCTA CAACTCCCAA GAACTCAGCT 
CCCACTACTA CCAAGAAGCC TGTTACTACA ACCAAGGAAC CAGTTCCTAC CACTCCCAAG 
GAGCCTGAAC CCACAACTCC CAAGGAACCA CCTCCAACCA CCAAGAAGCC TGAACCCACC 
ACCCCCAAGG AGCCTGAACC CACAACTCCC AAGGAGCCTG AACCCACCAC CCCCAAGGAG 
CCTGAACCCA CCACTCCCAA GGAGCCTGAA CCCACAACTC CCAAGGAACC ACCTCCAACC 
ACCAAGAAGC CTGAACCCAC CACCCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACCA CCACCAAGGA GCCTGAACCC ACCACCACCA AGGAGCCTGA ATCCACCACC 
CGCAAGGAGC CTGAACCCAC CACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACAACTCCCA AGGAGCCTGA ACCCACCACT 
CTCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACAACTCCCA AGGAGCCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACCA CGCCCAAGGA GCCTGAACCC ACAACCCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACCACTCT CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACCACTCCCA AGGAGCCTGA ACCCACCACT 
CTCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACCACTCT CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACCACCACCA AGGAGCCTGA ATCCACCACC 
CGCAAGGAGC CTGAACCCAC CACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGGA GCCTGAACCC ACCACGCCCA AGGAGCCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACAACCCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGAA ACCTGAACCC ACAACTCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC CACCCCCAAG GAGCCTGAAC CCACCACGCC CAAGGAGCCT 
GAACCCACAA CTCGCAAGGA GCCTGAACCT ACAACTCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG AAGCCTGAAC CCACCACCAC ATCACCCAAA 
ACCACTACTC TGAAAGCAAC AACTCTTGCA CCCAAAGTAA CTGCTCCAGC AGAAGAGATT 
CAGAACAAAC CTGAAGAAAC AACTCCTGCA TCAGAAGATT CTGATGATTC TAAAACAACT 
CTAAAACCAC AGAAGCCAAC CAAAGCACCC AAGCCTACCA AAAAGCCAAC CAAAGCACCC 
AAGAAGCCCA CCTCTACCAA AAAGCCAAAG ACACCAAAAA CAAGAAAACC AAAAACTACA 
CCATCTCCTC TAAAGACGAC TTCAGCAACA CCTGAACTGA ATACCACCCC TCTAGAAGTC 
ATGCTGCCAA CCACCACCAT CCCTAAACAA ACTCCAAACC CTGAAACAGC TGAAGTAAAT 
CCAGATCATG AAGATGCAGA TGGAGGTGAA GGAGAAAAAC CTCTGATTCC CGGGCCCCCT 
GTGCTATTCC CCACAGCTAT TCCAGGCACT GATCTTTTGG CCGGGAGACT CAATCAAGGC 
ATTAACATCA ATCCCATGCT TTCAGATGAG ACCAATTTAT GCAATGGTAA GCCAGTGGAT 
GGACTGACTA CGCTGCGCAA TGGGACATTA GTTGCATTTC GAGGTCATTA TTTCTGGATG 
CTGAATCCAT TCAGACCACC ATCTCCACCA CGCAGAATTA CCGAAGTTTG GGGTATTCCC 
TCTCCTATTG ACACAGTTTT TACTAGATGT AACTGTGAAG GAAAAACTTT CTTCTTTAAG 
GATTCTCAGT ACTGGCGCTT CACCAATGAT GTGGTAGATC CTGGGTATCC TAAACAAATT 
GTCAAAGGCT TTGGAGGACT AACAGGGAAG ATAGTGGCTG CTCTTTCAAT AGCCAAGTAC 
AAGGACAGGC CTGAATCTGT GTACTTCTTC AAGAGAGGTG GCAACATTCA GCAGTACACT 
TATAAACAGG AGCCAATGAA GAAGTGCACA GGGAGGCGGC CTGCCATCAA CTACTCGGTG 
TACGGGGAGG CAGCACAGGT CAGGAGGCGC CGCTTTGAGC GTGCTGTTGG ACCTTTCCAG 
ACACACACCT TCAGGATCCA TTACTCGGTG CCCATGAGAG TTTCTTATCA AGACAAAGGT 
TTCCTCCACA ATGAAGTCAA AGTGAGTACA ATGTGGAGAG GGTTTCCAAA TGTTGTTACT 
TCAGCGATAA CACTGCCTAA CATTAGGAAA CCTGATGGCT ATGATTACTA CGCCTTTTCC 
AAAGATCAAT ACTATAACAT TGATGTGCCT ACAAGAACAG CAAGAGCAAT TACCACACGT 
TCAGGGCAGA CCCTCTCCAA AATCTGGTAC AACTGTCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168521.1
CDS	80..3481
Translation	MGWKILPVCLSLLLPVVLIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTTELSCKGRCFESFARGRECDCDSQCKQYGKCCADYDSFCEEVKDNKKNTPKKKPNPEPPAVDEAGSGLDNGEFKLTPPPPDPPTTPHSKVTTSPKTTAAKPVTPKPSLAPNSETSKEASLASNKETTVETKETTATNKQSSASKKKTTSAKETRSAEKTSDKDVEPTSTTPKNSAPTTTKKPVTTTKEPVPTTPKEPEPTTPKEPPPTTKKPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPPPTTKKPEPTTPKEPEPTTPKEPEPTTTKEPEPTTTKEPESTTRKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTTKEPESTTRKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKKPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTRKEPEPTTPKEPEPTTPKEPEPTTPKKPEPTTTSPKTTTLKATTLAPKVTAPAEEIQNKPEETTPASEDSDDSKTTLKPQKPTKAPKPTKKPTKAPKKPTSTKKPKTPKTRKPKTTPSPLKTTSATPELNTTPLEVMLPTTTIPKQTPNPETAEVNPDHEDADGGEGEKPLIPGPPVLFPTAIPGTDLLAGRLNQGININPMLSDETNLCNGKPVDGLTTLRNGTLVAFRGHYFWMLNPFRPPSPPRRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDVVDPGYPKQIVKGFGGLTGKIVAALSIAKYKDRPESVYFFKRGGNIQQYTYKQEPMKKCTGRRPAINYSVYGEAAQVRRRRFERAVGPFQTHTFRIHYSVPMRVSYQDKGFLHNEVKVSTMWRGFPNVVTSAITLPNIRKPDGYDYYAFSKDQYYNIDVPTRTARAITTRSGQTLSKIWYNCP

Target ORF information:

RefSeq Version	XM_017313032.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313032.1

ORF Insert Sequence:

ATGGGGTGGA AAATACTTCC CGTCTGCTTG TCGCTGCTGC TTCCTGTTGT CTTGATTCAA 
CAGGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTACTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG TCCTGATTTC 
AAGAGAGTCT GCACTACAGA GCTCTCCTGC AAAGGACGCT GCTTCGAGTC CTTCGCTCGA 
GGGAGGGAGT GTGACTGTGA CTCCCAGTGT AAACAGTATG GCAAGTGCTG TGCAGATTAT 
GACAGCTTCT GTGAAGAAGT AAAAGATAAC AAGAAAAACA CTCCTAAAAA GAAACCCAAT 
CCAGAACCGC CGGCTGTGGA TGAAGCTGGA AGCGGGCTGG ATAATGGTGA GTTCAAGCTC 
ACCCCACCTC CTCCTGACCC TCCTACTACT CCACACAGCA AAGTCACCAC ATCTCCCAAG 
ACTACAGCAG CAAAACCAGT AACTCCTAAA CCCAGTCTTG CACCTAATTC TGAGACATCC 
AAAGAGGCAT CTTTAGCATC CAATAAAGAG ACAACAGTTG AAACGAAAGA GACTACTGCA 
ACAAATAAAC AATCTTCTGC CAGTAAAAAA AAGACGACTT CAGCTAAAGA GACACGGAGT 
GCAGAGAAAA CATCTGATAA AGATGTTGAG CCCACATCTA CAACTCCCAA GAACTCAGCT 
CCCACTACTA CCAAGAAGCC TGTTACTACA ACCAAGGAAC CAGTTCCTAC CACTCCCAAG 
GAGCCTGAAC CCACAACTCC CAAGGAACCA CCTCCAACCA CCAAGAAGCC TGAACCCACC 
ACCCCCAAGG AGCCTGAACC CACAACTCCC AAGGAGCCTG AACCCACCAC CCCCAAGGAG 
CCTGAACCCA CCACTCCCAA GGAGCCTGAA CCCACAACTC CCAAGGAACC ACCTCCAACC 
ACCAAGAAGC CTGAACCCAC CACCCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACCA CCACCAAGGA GCCTGAACCC ACCACCACCA AGGAGCCTGA ATCCACCACC 
CGCAAGGAGC CTGAACCCAC CACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACAACTCCCA AGGAGCCTGA ACCCACCACT 
CTCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACAACTCCCA AGGAGCCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACCA CGCCCAAGGA GCCTGAACCC ACAACCCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACCACTCT CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACCACTCCCA AGGAGCCTGA ACCCACCACT 
CTCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACCACTCT CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACCACCACCA AGGAGCCTGA ATCCACCACC 
CGCAAGGAGC CTGAACCCAC CACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGGA GCCTGAACCC ACCACGCCCA AGGAGCCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACAACCCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGAA ACCTGAACCC ACAACTCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC CACCCCCAAG GAGCCTGAAC CCACCACGCC CAAGGAGCCT 
GAACCCACAA CTCGCAAGGA GCCTGAACCT ACAACTCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG AAGCCTGAAC CCACCACCAC ATCACCCAAA 
ACCACTACTC TGAAAGCAAC AACTCTTGCA CCCAAAGTAA CTGCTCCAGC AGAAGAGATT 
CAGAACAAAC CTGAAGAAAC AACTCCTGCA TCAGAAGATT CTGATGATTC TAAAACAACT 
CTAAAACCAC AGAAGCCAAC CAAAGCACCC AAGCCTACCA AAAAGCCAAC CAAAGCACCC 
AAGAAGCCCA CCTCTACCAA AAAGCCAAAG ACACCAAAAA CAAGAAAACC AAAAACTACA 
CCATCTCCTC TAAAGACGAC TTCAGCAACA CCTGAACTGA ATACCACCCC TCTAGAAGTC 
ATGCTGCCAA CCACCACCAT CCCTAAACAA ACTCCAAACC CTGAAACAGC TGAAGTAAAT 
CCAGATCATG AAGATGCAGA TGGAGGTGAA GGAGAAAAAC CTCTGATTCC CGGGCCCCCT 
GTGCTATTCC CCACAGCTAT TCCAGGCACT GATCTTTTGG CCGGGAGACT CAATCAAGGC 
ATTAACATCA ATCCCATGCT TTCAGATGAG ACCAATTTAT GCAATGGTAA GCCAGTGGAT 
GGACTGACTA CGCTGCGCAA TGGGACATTA GTTGCATTTC GAGGTCATTA TTTCTGGATG 
CTGAATCCAT TCAGACCACC ATCTCCACCA CGCAGAATTA CCGAAGTTTG GGGTATTCCC 
TCTCCTATTG ACACAGTTTT TACTAGATGT AACTGTGAAG GAAAAACTTT CTTCTTTAAG 
GATTCTCAGT ACTGGCGCTT CACCAATGAT GTGGTAGATC CTGGGTATCC TAAACAAATT 
GTCAAAGGCT TTGGAGGACT AACAGGGAAG ATAGTGGCTG CTCTTTCAAT AGCCAAGTAC 
AAGGACAGGC CTGAATCTGT GTACTTCTTC AAGAGAGGTG GCAACATTCA GCAGTACACT 
TATAAACAGG AGCCAATGAA GAAGTGCACA GGGAGGCGGC CTGCCATCAA CTACTCGGTG 
TACGGGGAGG CAGCACAGGT CAGGAGGCGC CGCTTTGAGC GTGCTGTTGG ACCTTTCCAG 
ACACACACCT TCAGGATCCA TTACTCGGTG CCCATGAGAG TTTCTTATCA AGACAAAGGT 
TTCCTCCACA ATGAAGTCAA AGTGAGTACA ATGTGGAGAG GGTTTCCAAA TGTTGTTACT 
TCAGCGATAA CACTGCCTAA CATTAGGAAA CCTGATGGCT ATGATTACTA CGCCTTTTCC 
AAAGATCAAT ACTATAACAT TGATGTGCCT ACAAGAACAG CAAGAGCAAT TACCACACGT 
TCAGGGCAGA CCCTCTCCAA AATCTGGTAC AACTGTCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prg4 ( XM_006529963.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530026.1 Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu28504
Clone ID Related Accession (Same CDS sequence)	XM_017313032.1 , XM_006529963.1 , XM_017313032.2
Accession Version	XM_006529963.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	proteoglycan 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTGGA AAATACTTCC CGTCTGCTTG TCGCTGCTGC TTCCTGTTGT CTTGATTCAA 
CAGGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTACTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG TCCTGATTTC 
AAGAGAGTCT GCACTACAGA GCTCTCCTGC AAAGGACGCT GCTTCGAGTC CTTCGCTCGA 
GGGAGGGAGT GTGACTGTGA CTCCCAGTGT AAACAGTATG GCAAGTGCTG TGCAGATTAT 
GACAGCTTCT GTGAAGAAGT AAAAGATAAC AAGAAAAACA CTCCTAAAAA GAAACCCAAT 
CCAGAACCGC CGGCTGTGGA TGAAGCTGGA AGCGGGCTGG ATAATGGTGA GTTCAAGCTC 
ACCCCACCTC CTCCTGACCC TCCTACTACT CCACACAGCA AAGTCACCAC ATCTCCCAAG 
ACTACAGCAG CAAAACCAGT AACTCCTAAA CCCAGTCTTG CACCTAATTC TGAGACATCC 
AAAGAGGCAT CTTTAGCATC CAATAAAGAG ACAACAGTTG AAACGAAAGA GACTACTGCA 
ACAAATAAAC AATCTTCTGC CAGTAAAAAA AAGACGACTT CAGCTAAAGA GACACGGAGT 
GCAGAGAAAA CATCTGATAA AGATGTTGAG CCCACATCTA CAACTCCCAA GAACTCAGCT 
CCCACTACTA CCAAGAAGCC TGTTACTACA ACCAAGGAAC CAGTTCCTAC CACTCCCAAG 
GAGCCTGAAC CCACAACTCC CAAGGAACCA CCTCCAACCA CCAAGAAGCC TGAACCCACC 
ACCCCCAAGG AGCCTGAACC CACAACTCCC AAGGAGCCTG AACCCACCAC CCCCAAGGAG 
CCTGAACCCA CCACTCCCAA GGAGCCTGAA CCCACAACTC CCAAGGAACC ACCTCCAACC 
ACCAAGAAGC CTGAACCCAC CACCCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACCA CCACCAAGGA GCCTGAACCC ACCACCACCA AGGAGCCTGA ATCCACCACC 
CGCAAGGAGC CTGAACCCAC CACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACAACTCCCA AGGAGCCTGA ACCCACCACT 
CTCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACAACTCCCA AGGAGCCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACCA CGCCCAAGGA GCCTGAACCC ACAACCCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACCACTCT CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACCACTCCCA AGGAGCCTGA ACCCACCACT 
CTCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACCACTCT CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACCACCACCA AGGAGCCTGA ATCCACCACC 
CGCAAGGAGC CTGAACCCAC CACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGGA GCCTGAACCC ACCACGCCCA AGGAGCCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACAACCCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGAA ACCTGAACCC ACAACTCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC CACCCCCAAG GAGCCTGAAC CCACCACGCC CAAGGAGCCT 
GAACCCACAA CTCGCAAGGA GCCTGAACCT ACAACTCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG AAGCCTGAAC CCACCACCAC ATCACCCAAA 
ACCACTACTC TGAAAGCAAC AACTCTTGCA CCCAAAGTAA CTGCTCCAGC AGAAGAGATT 
CAGAACAAAC CTGAAGAAAC AACTCCTGCA TCAGAAGATT CTGATGATTC TAAAACAACT 
CTAAAACCAC AGAAGCCAAC CAAAGCACCC AAGCCTACCA AAAAGCCAAC CAAAGCACCC 
AAGAAGCCCA CCTCTACCAA AAAGCCAAAG ACACCAAAAA CAAGAAAACC AAAAACTACA 
CCATCTCCTC TAAAGACGAC TTCAGCAACA CCTGAACTGA ATACCACCCC TCTAGAAGTC 
ATGCTGCCAA CCACCACCAT CCCTAAACAA ACTCCAAACC CTGAAACAGC TGAAGTAAAT 
CCAGATCATG AAGATGCAGA TGGAGGTGAA GGAGAAAAAC CTCTGATTCC CGGGCCCCCT 
GTGCTATTCC CCACAGCTAT TCCAGGCACT GATCTTTTGG CCGGGAGACT CAATCAAGGC 
ATTAACATCA ATCCCATGCT TTCAGATGAG ACCAATTTAT GCAATGGTAA GCCAGTGGAT 
GGACTGACTA CGCTGCGCAA TGGGACATTA GTTGCATTTC GAGGTCATTA TTTCTGGATG 
CTGAATCCAT TCAGACCACC ATCTCCACCA CGCAGAATTA CCGAAGTTTG GGGTATTCCC 
TCTCCTATTG ACACAGTTTT TACTAGATGT AACTGTGAAG GAAAAACTTT CTTCTTTAAG 
GATTCTCAGT ACTGGCGCTT CACCAATGAT GTGGTAGATC CTGGGTATCC TAAACAAATT 
GTCAAAGGCT TTGGAGGACT AACAGGGAAG ATAGTGGCTG CTCTTTCAAT AGCCAAGTAC 
AAGGACAGGC CTGAATCTGT GTACTTCTTC AAGAGAGGTG GCAACATTCA GCAGTACACT 
TATAAACAGG AGCCAATGAA GAAGTGCACA GGGAGGCGGC CTGCCATCAA CTACTCGGTG 
TACGGGGAGG CAGCACAGGT CAGGAGGCGC CGCTTTGAGC GTGCTGTTGG ACCTTTCCAG 
ACACACACCT TCAGGATCCA TTACTCGGTG CCCATGAGAG TTTCTTATCA AGACAAAGGT 
TTCCTCCACA ATGAAGTCAA AGTGAGTACA ATGTGGAGAG GGTTTCCAAA TGTTGTTACT 
TCAGCGATAA CACTGCCTAA CATTAGGAAA CCTGATGGCT ATGATTACTA CGCCTTTTCC 
AAAGATCAAT ACTATAACAT TGATGTGCCT ACAAGAACAG CAAGAGCAAT TACCACACGT 
TCAGGGCAGA CCCTCTCCAA AATCTGGTAC AACTGTCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530026.1
CDS	97..3498
Translation	MGWKILPVCLSLLLPVVLIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTTELSCKGRCFESFARGRECDCDSQCKQYGKCCADYDSFCEEVKDNKKNTPKKKPNPEPPAVDEAGSGLDNGEFKLTPPPPDPPTTPHSKVTTSPKTTAAKPVTPKPSLAPNSETSKEASLASNKETTVETKETTATNKQSSASKKKTTSAKETRSAEKTSDKDVEPTSTTPKNSAPTTTKKPVTTTKEPVPTTPKEPEPTTPKEPPPTTKKPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPPPTTKKPEPTTPKEPEPTTPKEPEPTTTKEPEPTTTKEPESTTRKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTTKEPESTTRKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKKPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTRKEPEPTTPKEPEPTTPKEPEPTTPKKPEPTTTSPKTTTLKATTLAPKVTAPAEEIQNKPEETTPASEDSDDSKTTLKPQKPTKAPKPTKKPTKAPKKPTSTKKPKTPKTRKPKTTPSPLKTTSATPELNTTPLEVMLPTTTIPKQTPNPETAEVNPDHEDADGGEGEKPLIPGPPVLFPTAIPGTDLLAGRLNQGININPMLSDETNLCNGKPVDGLTTLRNGTLVAFRGHYFWMLNPFRPPSPPRRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDVVDPGYPKQIVKGFGGLTGKIVAALSIAKYKDRPESVYFFKRGGNIQQYTYKQEPMKKCTGRRPAINYSVYGEAAQVRRRRFERAVGPFQTHTFRIHYSVPMRVSYQDKGFLHNEVKVSTMWRGFPNVVTSAITLPNIRKPDGYDYYAFSKDQYYNIDVPTRTARAITTRSGQTLSKIWYNCP

Target ORF information:

RefSeq Version	XM_006529963.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529963.1

ORF Insert Sequence:

ATGGGGTGGA AAATACTTCC CGTCTGCTTG TCGCTGCTGC TTCCTGTTGT CTTGATTCAA 
CAGGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTACTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG TCCTGATTTC 
AAGAGAGTCT GCACTACAGA GCTCTCCTGC AAAGGACGCT GCTTCGAGTC CTTCGCTCGA 
GGGAGGGAGT GTGACTGTGA CTCCCAGTGT AAACAGTATG GCAAGTGCTG TGCAGATTAT 
GACAGCTTCT GTGAAGAAGT AAAAGATAAC AAGAAAAACA CTCCTAAAAA GAAACCCAAT 
CCAGAACCGC CGGCTGTGGA TGAAGCTGGA AGCGGGCTGG ATAATGGTGA GTTCAAGCTC 
ACCCCACCTC CTCCTGACCC TCCTACTACT CCACACAGCA AAGTCACCAC ATCTCCCAAG 
ACTACAGCAG CAAAACCAGT AACTCCTAAA CCCAGTCTTG CACCTAATTC TGAGACATCC 
AAAGAGGCAT CTTTAGCATC CAATAAAGAG ACAACAGTTG AAACGAAAGA GACTACTGCA 
ACAAATAAAC AATCTTCTGC CAGTAAAAAA AAGACGACTT CAGCTAAAGA GACACGGAGT 
GCAGAGAAAA CATCTGATAA AGATGTTGAG CCCACATCTA CAACTCCCAA GAACTCAGCT 
CCCACTACTA CCAAGAAGCC TGTTACTACA ACCAAGGAAC CAGTTCCTAC CACTCCCAAG 
GAGCCTGAAC CCACAACTCC CAAGGAACCA CCTCCAACCA CCAAGAAGCC TGAACCCACC 
ACCCCCAAGG AGCCTGAACC CACAACTCCC AAGGAGCCTG AACCCACCAC CCCCAAGGAG 
CCTGAACCCA CCACTCCCAA GGAGCCTGAA CCCACAACTC CCAAGGAACC ACCTCCAACC 
ACCAAGAAGC CTGAACCCAC CACCCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACCA CCACCAAGGA GCCTGAACCC ACCACCACCA AGGAGCCTGA ATCCACCACC 
CGCAAGGAGC CTGAACCCAC CACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACAACTCCCA AGGAGCCTGA ACCCACCACT 
CTCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACAACTCCCA AGGAGCCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACCA CGCCCAAGGA GCCTGAACCC ACAACCCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACCACTCT CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACCACTCCCA AGGAGCCTGA ACCCACCACT 
CTCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACCACTCT CAAGGAGCCT 
GAACCTACAA CCCCCAAGGA GCCTGAACCC ACCACCACCA AGGAGCCTGA ATCCACCACC 
CGCAAGGAGC CTGAACCCAC CACTCCCAAG GAGCCTGAAC CCACAACTCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGGA GCCTGAACCC ACCACGCCCA AGGAGCCTGA ACCCACAACT 
CCCAAGGAGC CTGAACCCAC CACGCCCAAG GAGCCTGAAC CCACAACCCC CAAGGAGCCT 
GAACCCACAA CTCCCAAGAA ACCTGAACCC ACAACTCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC CACCCCCAAG GAGCCTGAAC CCACCACGCC CAAGGAGCCT 
GAACCCACAA CTCGCAAGGA GCCTGAACCT ACAACTCCCA AGGAGCCTGA ACCTACAACC 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG AAGCCTGAAC CCACCACCAC ATCACCCAAA 
ACCACTACTC TGAAAGCAAC AACTCTTGCA CCCAAAGTAA CTGCTCCAGC AGAAGAGATT 
CAGAACAAAC CTGAAGAAAC AACTCCTGCA TCAGAAGATT CTGATGATTC TAAAACAACT 
CTAAAACCAC AGAAGCCAAC CAAAGCACCC AAGCCTACCA AAAAGCCAAC CAAAGCACCC 
AAGAAGCCCA CCTCTACCAA AAAGCCAAAG ACACCAAAAA CAAGAAAACC AAAAACTACA 
CCATCTCCTC TAAAGACGAC TTCAGCAACA CCTGAACTGA ATACCACCCC TCTAGAAGTC 
ATGCTGCCAA CCACCACCAT CCCTAAACAA ACTCCAAACC CTGAAACAGC TGAAGTAAAT 
CCAGATCATG AAGATGCAGA TGGAGGTGAA GGAGAAAAAC CTCTGATTCC CGGGCCCCCT 
GTGCTATTCC CCACAGCTAT TCCAGGCACT GATCTTTTGG CCGGGAGACT CAATCAAGGC 
ATTAACATCA ATCCCATGCT TTCAGATGAG ACCAATTTAT GCAATGGTAA GCCAGTGGAT 
GGACTGACTA CGCTGCGCAA TGGGACATTA GTTGCATTTC GAGGTCATTA TTTCTGGATG 
CTGAATCCAT TCAGACCACC ATCTCCACCA CGCAGAATTA CCGAAGTTTG GGGTATTCCC 
TCTCCTATTG ACACAGTTTT TACTAGATGT AACTGTGAAG GAAAAACTTT CTTCTTTAAG 
GATTCTCAGT ACTGGCGCTT CACCAATGAT GTGGTAGATC CTGGGTATCC TAAACAAATT 
GTCAAAGGCT TTGGAGGACT AACAGGGAAG ATAGTGGCTG CTCTTTCAAT AGCCAAGTAC 
AAGGACAGGC CTGAATCTGT GTACTTCTTC AAGAGAGGTG GCAACATTCA GCAGTACACT 
TATAAACAGG AGCCAATGAA GAAGTGCACA GGGAGGCGGC CTGCCATCAA CTACTCGGTG 
TACGGGGAGG CAGCACAGGT CAGGAGGCGC CGCTTTGAGC GTGCTGTTGG ACCTTTCCAG 
ACACACACCT TCAGGATCCA TTACTCGGTG CCCATGAGAG TTTCTTATCA AGACAAAGGT 
TTCCTCCACA ATGAAGTCAA AGTGAGTACA ATGTGGAGAG GGTTTCCAAA TGTTGTTACT 
TCAGCGATAA CACTGCCTAA CATTAGGAAA CCTGATGGCT ATGATTACTA CGCCTTTTCC 
AAAGATCAAT ACTATAACAT TGATGTGCCT ACAAGAACAG CAAGAGCAAT TACCACACGT 
TCAGGGCAGA CCCTCTCCAA AATCTGGTAC AACTGTCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prg4 ( XM_006529966.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530029.1 Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu28506
Clone ID Related Accession (Same CDS sequence)	XM_006529966.1
Accession Version	XM_006529966.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	proteoglycan 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTGGA AAATACTTCC CGTCTGCTTG TCGCTGCTGC TTCCTGTTGT CTTGATTCAA 
CAGGTTTCAT CTCAAGAGCT CTCCTGCAAA GGACGCTGCT TCGAGTCCTT CGCTCGAGGG 
AGGGAGTGTG ACTGTGACTC CCAGTGTAAA CAGTATGGCA AGTGCTGTGC AGATTATGAC 
AGCTTCTGTG AAGAAGTAAA AGATAACAAG AAAAACACTC CTAAAAAGAA ACCCAATCCA 
GAACCGCCGG CTGTGGATGA AGCTGGAAGC GGGCTGGATA ATGGTGAGTT CAAGCTCACC 
CCACCTCCTC CTGACCCTCC TACTACTCCA CACAGCAAAG TCACCACATC TCCCAAGACT 
ACAGCAGCAA AACCAGTAAC TCCTAAACCC AGTCTTGCAC CTAATTCTGA GACATCCAAA 
GAGGCATCTT TAGCATCCAA TAAAGAGACA ACAGTTGAAA CGAAAGAGAC TACTGCAACA 
AATAAACAAT CTTCTGCCAG TAAAAAAAAG ACGACTTCAG CTAAAGAGAC ACGGAGTGCA 
GAGAAAACAT CTGATAAAGA TGTTGAGCCC ACATCTACAA CTCCCAAGAA CTCAGCTCCC 
ACTACTACCA AGAAGCCTGT TACTACAACC AAGGAACCAG TTCCTACCAC TCCCAAGGAG 
CCTGAACCCA CAACTCCCAA GGAACCACCT CCAACCACCA AGAAGCCTGA ACCCACCACC 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACCACCCC CAAGGAGCCT 
GAACCCACCA CTCCCAAGGA GCCTGAACCC ACAACTCCCA AGGAACCACC TCCAACCACC 
AAGAAGCCTG AACCCACCAC CCCCAAGGAG CCTGAACCCA CAACTCCCAA GGAGCCTGAA 
CCCACCACCA CCAAGGAGCC TGAACCCACC ACCACCAAGG AGCCTGAATC CACCACCCGC 
AAGGAGCCTG AACCCACCAC TCCCAAGGAG CCTGAACCCA CAACTCCCAA GGAGCCTGAA 
CCTACAACCC CCAAGGAGCC TGAACCCACA ACTCCCAAGG AGCCTGAACC CACCACTCTC 
AAGGAGCCTG AACCCACCAC GCCCAAGGAG CCTGAACCCA CAACTCCCAA GGAGCCTGAA 
CCTACAACCC CCAAGGAGCC TGAACCCACA ACTCCCAAGG AGCCTGAACC CACAACTCCC 
AAGGAGCCTG AACCCACAAC TCCCAAGGAG CCTGAACCCA CAACTCCCAA GGAGCCTGAA 
CCCACCACGC CCAAGGAGCC TGAACCCACA ACCCCCAAGG AGCCTGAACC TACAACCCCC 
AAGGAGCCTG AACCCACAAC TCCCAAGGAG CCTGAACCCA CCACTCTCAA GGAGCCTGAA 
CCTACAACCC CCAAGGAGCC TGAACCCACC ACTCCCAAGG AGCCTGAACC CACCACTCTC 
AAGGAGCCTG AACCCACCAC GCCCAAGGAG CCTGAACCCA CCACTCTCAA GGAGCCTGAA 
CCTACAACCC CCAAGGAGCC TGAACCCACC ACCACCAAGG AGCCTGAATC CACCACCCGC 
AAGGAGCCTG AACCCACCAC TCCCAAGGAG CCTGAACCCA CAACTCCCAA GGAGCCTGAA 
CCCACAACTC CCAAGGAGCC TGAACCCACC ACGCCCAAGG AGCCTGAACC CACAACTCCC 
AAGGAGCCTG AACCCACCAC GCCCAAGGAG CCTGAACCCA CAACCCCCAA GGAGCCTGAA 
CCCACAACTC CCAAGAAACC TGAACCCACA ACTCCCAAGG AGCCTGAACC TACAACCCCC 
AAGGAGCCTG AACCCACCAC CCCCAAGGAG CCTGAACCCA CCACGCCCAA GGAGCCTGAA 
CCCACAACTC GCAAGGAGCC TGAACCTACA ACTCCCAAGG AGCCTGAACC TACAACCCCC 
AAGGAGCCTG AACCCACAAC TCCCAAGAAG CCTGAACCCA CCACCACATC ACCCAAAACC 
ACTACTCTGA AAGCAACAAC TCTTGCACCC AAAGTAACTG CTCCAGCAGA AGAGATTCAG 
AACAAACCTG AAGAAACAAC TCCTGCATCA GAAGATTCTG ATGATTCTAA AACAACTCTA 
AAACCACAGA AGCCAACCAA AGCACCCAAG CCTACCAAAA AGCCAACCAA AGCACCCAAG 
AAGCCCACCT CTACCAAAAA GCCAAAGACA CCAAAAACAA GAAAACCAAA AACTACACCA 
TCTCCTCTAA AGACGACTTC AGCAACACCT GAACTGAATA CCACCCCTCT AGAAGTCATG 
CTGCCAACCA CCACCATCCC TAAACAAACT CCAAACCCTG AAACAGCTGA AGTAAATCCA 
GATCATGAAG ATGCAGATGG AGGTGAAGGA GAAAAACCTC TGATTCCCGG GCCCCCTGTG 
CTATTCCCCA CAGCTATTCC AGGCACTGAT CTTTTGGCCG GGAGACTCAA TCAAGGCATT 
AACATCAATC CCATGCTTTC AGATGAGACC AATTTATGCA ATGGTAAGCC AGTGGATGGA 
CTGACTACGC TGCGCAATGG GACATTAGTT GCATTTCGAG GTCATTATTT CTGGATGCTG 
AATCCATTCA GACCACCATC TCCACCACGC AGAATTACCG AAGTTTGGGG TATTCCCTCT 
CCTATTGACA CAGTTTTTAC TAGATGTAAC TGTGAAGGAA AAACTTTCTT CTTTAAGGAT 
TCTCAGTACT GGCGCTTCAC CAATGATGTG GTAGATCCTG GGTATCCTAA ACAAATTGTC 
AAAGGCTTTG GAGGACTAAC AGGGAAGATA GTGGCTGCTC TTTCAATAGC CAAGTACAAG 
GACAGGCCTG AATCTGTGTA CTTCTTCAAG AGAGGTGGCA ACATTCAGCA GTACACTTAT 
AAACAGGAGC CAATGAAGAA GTGCACAGGG AGGCGGCCTG CCATCAACTA CTCGGTGTAC 
GGGGAGGCAG CACAGGTCAG GAGGCGCCGC TTTGAGCGTG CTGTTGGACC TTTCCAGACA 
CACACCTTCA GGATCCATTA CTCGGTGCCC ATGAGAGTTT CTTATCAAGA CAAAGGTTTC 
CTCCACAATG AAGTCAAAGT GAGTACAATG TGGAGAGGGT TTCCAAATGT TGTTACTTCA 
GCGATAACAC TGCCTAACAT TAGGAAACCT GATGGCTATG ATTACTACGC CTTTTCCAAA 
GATCAATACT ATAACATTGA TGTGCCTACA AGAACAGCAA GAGCAATTAC CACACGTTCA 
GGGCAGACCC TCTCCAAAAT CTGGTACAAC TGTCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530029.1
CDS	97..3375
Translation	MGWKILPVCLSLLLPVVLIQQVSSQELSCKGRCFESFARGRECDCDSQCKQYGKCCADYDSFCEEVKDNKKNTPKKKPNPEPPAVDEAGSGLDNGEFKLTPPPPDPPTTPHSKVTTSPKTTAAKPVTPKPSLAPNSETSKEASLASNKETTVETKETTATNKQSSASKKKTTSAKETRSAEKTSDKDVEPTSTTPKNSAPTTTKKPVTTTKEPVPTTPKEPEPTTPKEPPPTTKKPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPPPTTKKPEPTTPKEPEPTTPKEPEPTTTKEPEPTTTKEPESTTRKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTLKEPEPTTPKEPEPTTTKEPESTTRKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKKPEPTTPKEPEPTTPKEPEPTTPKEPEPTTPKEPEPTTRKEPEPTTPKEPEPTTPKEPEPTTPKKPEPTTTSPKTTTLKATTLAPKVTAPAEEIQNKPEETTPASEDSDDSKTTLKPQKPTKAPKPTKKPTKAPKKPTSTKKPKTPKTRKPKTTPSPLKTTSATPELNTTPLEVMLPTTTIPKQTPNPETAEVNPDHEDADGGEGEKPLIPGPPVLFPTAIPGTDLLAGRLNQGININPMLSDETNLCNGKPVDGLTTLRNGTLVAFRGHYFWMLNPFRPPSPPRRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDVVDPGYPKQIVKGFGGLTGKIVAALSIAKYKDRPESVYFFKRGGNIQQYTYKQEPMKKCTGRRPAINYSVYGEAAQVRRRRFERAVGPFQTHTFRIHYSVPMRVSYQDKGFLHNEVKVSTMWRGFPNVVTSAITLPNIRKPDGYDYYAFSKDQYYNIDVPTRTARAITTRSGQTLSKIWYNCP

Target ORF information:

RefSeq Version	XM_006529966.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529966.1

ORF Insert Sequence:

ATGGGGTGGA AAATACTTCC CGTCTGCTTG TCGCTGCTGC TTCCTGTTGT CTTGATTCAA 
CAGGTTTCAT CTCAAGAGCT CTCCTGCAAA GGACGCTGCT TCGAGTCCTT CGCTCGAGGG 
AGGGAGTGTG ACTGTGACTC CCAGTGTAAA CAGTATGGCA AGTGCTGTGC AGATTATGAC 
AGCTTCTGTG AAGAAGTAAA AGATAACAAG AAAAACACTC CTAAAAAGAA ACCCAATCCA 
GAACCGCCGG CTGTGGATGA AGCTGGAAGC GGGCTGGATA ATGGTGAGTT CAAGCTCACC 
CCACCTCCTC CTGACCCTCC TACTACTCCA CACAGCAAAG TCACCACATC TCCCAAGACT 
ACAGCAGCAA AACCAGTAAC TCCTAAACCC AGTCTTGCAC CTAATTCTGA GACATCCAAA 
GAGGCATCTT TAGCATCCAA TAAAGAGACA ACAGTTGAAA CGAAAGAGAC TACTGCAACA 
AATAAACAAT CTTCTGCCAG TAAAAAAAAG ACGACTTCAG CTAAAGAGAC ACGGAGTGCA 
GAGAAAACAT CTGATAAAGA TGTTGAGCCC ACATCTACAA CTCCCAAGAA CTCAGCTCCC 
ACTACTACCA AGAAGCCTGT TACTACAACC AAGGAACCAG TTCCTACCAC TCCCAAGGAG 
CCTGAACCCA CAACTCCCAA GGAACCACCT CCAACCACCA AGAAGCCTGA ACCCACCACC 
CCCAAGGAGC CTGAACCCAC AACTCCCAAG GAGCCTGAAC CCACCACCCC CAAGGAGCCT 
GAACCCACCA CTCCCAAGGA GCCTGAACCC ACAACTCCCA AGGAACCACC TCCAACCACC 
AAGAAGCCTG AACCCACCAC CCCCAAGGAG CCTGAACCCA CAACTCCCAA GGAGCCTGAA 
CCCACCACCA CCAAGGAGCC TGAACCCACC ACCACCAAGG AGCCTGAATC CACCACCCGC 
AAGGAGCCTG AACCCACCAC TCCCAAGGAG CCTGAACCCA CAACTCCCAA GGAGCCTGAA 
CCTACAACCC CCAAGGAGCC TGAACCCACA ACTCCCAAGG AGCCTGAACC CACCACTCTC 
AAGGAGCCTG AACCCACCAC GCCCAAGGAG CCTGAACCCA CAACTCCCAA GGAGCCTGAA 
CCTACAACCC CCAAGGAGCC TGAACCCACA ACTCCCAAGG AGCCTGAACC CACAACTCCC 
AAGGAGCCTG AACCCACAAC TCCCAAGGAG CCTGAACCCA CAACTCCCAA GGAGCCTGAA 
CCCACCACGC CCAAGGAGCC TGAACCCACA ACCCCCAAGG AGCCTGAACC TACAACCCCC 
AAGGAGCCTG AACCCACAAC TCCCAAGGAG CCTGAACCCA CCACTCTCAA GGAGCCTGAA 
CCTACAACCC CCAAGGAGCC TGAACCCACC ACTCCCAAGG AGCCTGAACC CACCACTCTC 
AAGGAGCCTG AACCCACCAC GCCCAAGGAG CCTGAACCCA CCACTCTCAA GGAGCCTGAA 
CCTACAACCC CCAAGGAGCC TGAACCCACC ACCACCAAGG AGCCTGAATC CACCACCCGC 
AAGGAGCCTG AACCCACCAC TCCCAAGGAG CCTGAACCCA CAACTCCCAA GGAGCCTGAA 
CCCACAACTC CCAAGGAGCC TGAACCCACC ACGCCCAAGG AGCCTGAACC CACAACTCCC 
AAGGAGCCTG AACCCACCAC GCCCAAGGAG CCTGAACCCA CAACCCCCAA GGAGCCTGAA 
CCCACAACTC CCAAGAAACC TGAACCCACA ACTCCCAAGG AGCCTGAACC TACAACCCCC 
AAGGAGCCTG AACCCACCAC CCCCAAGGAG CCTGAACCCA CCACGCCCAA GGAGCCTGAA 
CCCACAACTC GCAAGGAGCC TGAACCTACA ACTCCCAAGG AGCCTGAACC TACAACCCCC 
AAGGAGCCTG AACCCACAAC TCCCAAGAAG CCTGAACCCA CCACCACATC ACCCAAAACC 
ACTACTCTGA AAGCAACAAC TCTTGCACCC AAAGTAACTG CTCCAGCAGA AGAGATTCAG 
AACAAACCTG AAGAAACAAC TCCTGCATCA GAAGATTCTG ATGATTCTAA AACAACTCTA 
AAACCACAGA AGCCAACCAA AGCACCCAAG CCTACCAAAA AGCCAACCAA AGCACCCAAG 
AAGCCCACCT CTACCAAAAA GCCAAAGACA CCAAAAACAA GAAAACCAAA AACTACACCA 
TCTCCTCTAA AGACGACTTC AGCAACACCT GAACTGAATA CCACCCCTCT AGAAGTCATG 
CTGCCAACCA CCACCATCCC TAAACAAACT CCAAACCCTG AAACAGCTGA AGTAAATCCA 
GATCATGAAG ATGCAGATGG AGGTGAAGGA GAAAAACCTC TGATTCCCGG GCCCCCTGTG 
CTATTCCCCA CAGCTATTCC AGGCACTGAT CTTTTGGCCG GGAGACTCAA TCAAGGCATT 
AACATCAATC CCATGCTTTC AGATGAGACC AATTTATGCA ATGGTAAGCC AGTGGATGGA 
CTGACTACGC TGCGCAATGG GACATTAGTT GCATTTCGAG GTCATTATTT CTGGATGCTG 
AATCCATTCA GACCACCATC TCCACCACGC AGAATTACCG AAGTTTGGGG TATTCCCTCT 
CCTATTGACA CAGTTTTTAC TAGATGTAAC TGTGAAGGAA AAACTTTCTT CTTTAAGGAT 
TCTCAGTACT GGCGCTTCAC CAATGATGTG GTAGATCCTG GGTATCCTAA ACAAATTGTC 
AAAGGCTTTG GAGGACTAAC AGGGAAGATA GTGGCTGCTC TTTCAATAGC CAAGTACAAG 
GACAGGCCTG AATCTGTGTA CTTCTTCAAG AGAGGTGGCA ACATTCAGCA GTACACTTAT 
AAACAGGAGC CAATGAAGAA GTGCACAGGG AGGCGGCCTG CCATCAACTA CTCGGTGTAC 
GGGGAGGCAG CACAGGTCAG GAGGCGCCGC TTTGAGCGTG CTGTTGGACC TTTCCAGACA 
CACACCTTCA GGATCCATTA CTCGGTGCCC ATGAGAGTTT CTTATCAAGA CAAAGGTTTC 
CTCCACAATG AAGTCAAAGT GAGTACAATG TGGAGAGGGT TTCCAAATGT TGTTACTTCA 
GCGATAACAC TGCCTAACAT TAGGAAACCT GATGGCTATG ATTACTACGC CTTTTCCAAA 
GATCAATACT ATAACATTGA TGTGCCTACA AGAACAGCAA GAGCAATTAC CACACGTTCA 
GGGCAGACCC TCTCCAAAAT CTGGTACAAC TGTCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Prg4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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