Acsbg1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acsbg1
Entrez Gene ID	94180
Full Name	acyl-CoA synthetase bubblegum family member 1
Synonyms	BG1,Bgm,E230019G03Rik,GR-LACS,Lpd,R75185
General protein information	Preferred Names acyl-CoA synthetase bubblegum family member 1 Names long-chain-fatty-acid--CoA ligase ACSBG1 bubblegum gonadotropin-regulated long chain acyl CoA synthetase lipidosin lipidosis-related protein lipidosin lipodisin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A5.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006511588.3	XP_006511651.1	long-chain-fatty-acid--CoA ligase ACSBG1 isoform X1
XM_030244753.1	XP_030100613.1	long-chain-fatty-acid--CoA ligase ACSBG1 isoform X2
XM_006511588.2	XP_006511651.1	long-chain-fatty-acid--CoA ligase ACSBG1 isoform X1
NM_053178.2	NP_444408.1	long-chain-fatty-acid--CoA ligase ACSBG1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100006023	XP_001344904.2
Homo sapiens (human)	ACSBG1	NP_055977.3
	LOC709676	XP_002804950.1
Bos taurus (cattle)	ACSBG1	NP_001019719.1
Canis lupus familiaris (dog)	ACSBG1	XP_005618497.1
Rattus norvegicus (Norway rat)	Acsbg1	NP_599216.1
Pan troglodytes (chimpanzee)	ACSBG1	XP_510525.3
Mus musculus (house mouse)	Acsbg1	NP_444408.1
Gallus gallus (chicken)	ACSBG1	XP_413747.3

Gene Ontology
Bibliography

Related articles in PubMed

RNF20 controls astrocytic differentiation through epigenetic regulation of STAT3 in the developing brain.
Liang Q, Xia W, Li W, Jiao J
Cell death and differentiation25(2)294-306(2018 Feb)

Analogous mechanism regulating formation of neocortical basal radial glia and cerebellar Bergmann glia.
Heng X, Guo Q, Leung AW, Li JY
eLife6(2017 May)

DISC1 regulates astrogenesis in the embryonic brain via modulation of RAS/MEK/ERK signaling through RASSF7.
Wang S, Liang Q, Qiao H, Li H, Shen T, Ji F, Jiao J
Development (Cambridge, England)143(15)2732-40(2016 08)

Conserved molecular signatures of neurogenesis in the hippocampal subgranular zone of rodents and primates.
Miller JA, Nathanson J, Franjic D, Shim S, Dalley RA, Shapouri S, Smith KA, Sunkin SM, Bernard A, Bennett JL, Lee CK, Hawrylycz MJ, Jones AR, Amaral DG, ?estan N, Gage FH, Lein ES
Development (Cambridge, England)140(22)4633-44(2013 Nov)

MEK Is a Key Regulator of Gliogenesis in the Developing Brain.
Li X, Newbern JM, Wu Y, Morgan-Smith M, Zhong J, Charron J, Snider WD
Neuron75(6)1035-50(2012 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data suggest that lipidosin was involved in fatty acid metabolism during reconstruction of the myelin sheath.
Title: Expression of an acyl-CoA synthetase, lipidosin, in astrocytes of the murine brain and its up-regulation during remyelination following cuprizone-induced demyelination.

Expression pattern and localization of bubblegum to microsomes.
Title: Murine bubblegum orthologue is a microsomal very long-chain acyl-CoA synthetase.

in neurons, mBG1-activated long-chain fatty acids are directed toward mitochondrial degradation
Title: The acyl-CoA synthetase "bubblegum" (lipidosin): further characterization and role in neuronal fatty acid beta-oxidation..

The following Acsbg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acsbg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09301	NM_053178.2 Latest version!	Mus musculus acyl-CoA synthetase bubblegum family member 1 (Acsbg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$426.30 $609.00
OMu43075	XM_006511588.3 Latest version!	Mus musculus acyl-CoA synthetase bubblegum family member 1 (Acsbg1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu43076	XM_030244753.1 Latest version!	Mus musculus acyl-CoA synthetase bubblegum family member 1 (Acsbg1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu43075	XM_006511588.2	Mus musculus acyl-CoA synthetase bubblegum family member 1 (Acsbg1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acsbg1 ( XM_006511588.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511651.1 Mus musculus acyl-CoA synthetase bubblegum family member 1 (Acsbg1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu43075
Clone ID Related Accession (Same CDS sequence)	XM_006511588.3 , XM_006511588.2
Accession Version	XM_006511588.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	long-chain-fatty-acid--CoA ligase ACSBG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511588.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGAAG TGCTTCTTCT CAGGTCTGCC AGCTCCATCC TGAGGACCGT GTTCGTAAGC 
AGACTGCTGC CAGGTGGGCC TGGGTGTGTT CGAAAACTTA GTTTGAACCT GAAGTACCAG 
CAAGGAATAA GGCCGAGTGT ACGAAGCAGC TCCTTAATTG ATGGGCAAAC ACTCTCCAAA 
GAGTCTCCAA GTCACGGTCT CGAGCTCTCA GCTCCAGAAA AGGCAAGGGC TGCCTCTTTG 
GATGGTGCAG AGGAAGCACT ATGGACAACC CGGGCGGATG GGAGAGTGCG CCTGCGCCTG 
GAACCTTTCT GTACTCAGCG TCCCTATACC GTGCACCAGA TGTTCTACGA GGCCCTAGAT 
AAGTACGGGA ACCTCAGTGC TCTGGGCTTC AAGCGCAAGG ACAAGTGGGA GCGTATCTCT 
TACTACCAGT ACTACCTGAT TGCACGCAAA GTAGCCAAAG GCTTCTTGAA GCTCGGCCTA 
GAGCGTGCCC ACAGCGTGGC GATCCTTGGC TTCAACTCTC CAGAATGGTT CTTCTCTGCA 
GTGGGCACAG TGTTCGCAGG GGGCATTGTC ACTGGCATCT ACACCACCAG CTCCCCGGAG 
GCCTGCCAGT ACATCTCTCA TGACTGCCGA GCCAATGTCA TCGTGGTTGA CACACAGAAG 
CAGCTGGAAA AGATCCTGAA GATCTGGAAA GACTTGCCAC ATCTAAAGGC AGTGGTGATA 
TACCAAGAAC CCCCTCCAAA GAAGATGGCT AACGTGTACA CGATGGAGGA GCTCATAGAA 
CTGGGGCAAG AAGTGCCTGA GGAGGCCCTA GATGCCATCA TCGATACCCA GCAACCCAAC 
CAGTGCTGTG TGCTCGTCTA CACATCAGGC ACCACGGGAA ACCCCAAGGG TGTGATGCTG 
AGTCAAGACA ATATCACATG GACAGCACGG TATGGCAGTC AGGCTGGGGA CATCCAGCCA 
GCAGAAGTGC AGCAGGAGGT AGTGGTCAGC TACTTGCCTC TCAGCCACAT CGCTGCCCAG 
ATCTACGACC TGTGGACAGG CATCCAGTGG GGAGCCCAGG TCTGCTTTGC AGATCCGGAC 
GCCCTGAAGG GGACCCTGGT AAACACACTG CGGGAGGTGG AGCCCACATC CCACATGGGA 
GTGCCGCGTG TGTGGGAGAA GATCATGGAA AGGATCCAGG AGGTGGCCGC TCAGTCTGGC 
TTCATCCGCC GCAAGATGCT GCTGTGGGCC ATGTCAGTCA CCTTGGAACA GAACCTTACC 
TGCCCTAGCA ATGACCTGAA GCCCTTCACC AGCAGACTGG CGGATTACCT AGTGTTAGCC 
AGGGTCCGTC AGGCTCTGGG CTTTGCCAAG TGTCAGAAAA ACTTCTACGG AGCAGCTCCG 
ATGACGGCAG AGACGCAGCG CTTCTTCCTG GGCCTCAACA TCCGCCTGTA TGCAGGCTAT 
GGCCTCAGCG AGAGCACAGG CCCCCACTTC ATGTCCAGCC CCTACAACTA TCGGCTTTAC 
AGCTCCGGCA GGGTGGTACC CGGCTGCCGG GTGAAGCTGG TGAATCAGGA TGCAGATGGC 
ATCGGTGAGA TCTGCCTGTG GGGCCGCACC ATCTTCATGG GTTATCTGAA CATGGAGGAC 
AAAACCTGTG AGGCCATTGA TTCGGAAGGC TGGCTGCACA CAGGTGACAT GGGCCGCCTG 
GATGCTGACG GCTTCCTCTA CATCACCGGG CGCCTCAAAG AATTAATCAT CACTGCGGGC 
GGCGAGAACG TGCCCCCAGT GCCTATCGAG GAGGCCGTGA AGATGGAGCT GCCCATCATC 
AGCAGTGCCA TGCTGATCGG GGACCAGAGG AAGTTCCTGT CCATGCTGCT AACCCTGAAG 
TGCACGCTGG ACCCAGAGAC GTCTGAGCCT ACAGACAGCC TGACCGAGCA AGCTGTGGAG 
TTCTGCCAGC GGGTGGGCAG CAAGGCCAGC ACCGTCTCCG AGATCGTGGG GCAGAGAGAC 
GAGGCTGTGT ACCAGGCCAT TCACGAAGGG ATCCAGAGGG TGAACGCGAA CGCCGCAGCC 
CGGCCCTACC ACATCCAGAA GTGGGCCATT CTCCAACGTG ACTTCTCCAT TTCAGGTGGA 
GAACTGGGAC CCACCATGAA ATTGAAACGG CTCACGGTTC TGGAGAAGTA CAAAGATATC 
ATCGATTCTT TTTATCAAGA GCAAAAACAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511651.1
CDS	334..2526
Translation	MPEVLLLRSASSILRTVFVSRLLPGGPGCVRKLSLNLKYQQGIRPSVRSSSLIDGQTLSKESPSHGLELSAPEKARAASLDGAEEALWTTRADGRVRLRLEPFCTQRPYTVHQMFYEALDKYGNLSALGFKRKDKWERISYYQYYLIARKVAKGFLKLGLERAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYISHDCRANVIVVDTQKQLEKILKIWKDLPHLKAVVIYQEPPPKKMANVYTMEELIELGQEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFADPDALKGTLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIRRKMLLWAMSVTLEQNLTCPSNDLKPFTSRLADYLVLARVRQALGFAKCQKNFYGAAPMTAETQRFFLGLNIRLYAGYGLSESTGPHFMSSPYNYRLYSSGRVVPGCRVKLVNQDADGIGEICLWGRTIFMGYLNMEDKTCEAIDSEGWLHTGDMGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKMELPIISSAMLIGDQRKFLSMLLTLKCTLDPETSEPTDSLTEQAVEFCQRVGSKASTVSEIVGQRDEAVYQAIHEGIQRVNANAAARPYHIQKWAILQRDFSISGGELGPTMKLKRLTVLEKYKDIIDSFYQEQKQ

Target ORF information:

RefSeq Version	XM_006511588.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus acyl-CoA synthetase bubblegum family member 1 (Acsbg1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511588.2

ORF Insert Sequence:

ATGCCAGAAG TGCTTCTTCT CAGGTCTGCC AGCTCCATCC TGAGGACCGT GTTCGTAAGC 
AGACTGCTGC CAGGTGGGCC TGGGTGTGTT CGAAAACTTA GTTTGAACCT GAAGTACCAG 
CAAGGAATAA GGCCGAGTGT ACGAAGCAGC TCCTTAATTG ATGGGCAAAC ACTCTCCAAA 
GAGTCTCCAA GTCACGGTCT CGAGCTCTCA GCTCCAGAAA AGGCAAGGGC TGCCTCTTTG 
GATGGTGCAG AGGAAGCACT ATGGACAACC CGGGCGGATG GGAGAGTGCG CCTGCGCCTG 
GAACCTTTCT GTACTCAGCG TCCCTATACC GTGCACCAGA TGTTCTACGA GGCCCTAGAT 
AAGTACGGGA ACCTCAGTGC TCTGGGCTTC AAGCGCAAGG ACAAGTGGGA GCGTATCTCT 
TACTACCAGT ACTACCTGAT TGCACGCAAA GTAGCCAAAG GCTTCTTGAA GCTCGGCCTA 
GAGCGTGCCC ACAGCGTGGC GATCCTTGGC TTCAACTCTC CAGAATGGTT CTTCTCTGCA 
GTGGGCACAG TGTTCGCAGG GGGCATTGTC ACTGGCATCT ACACCACCAG CTCCCCGGAG 
GCCTGCCAGT ACATCTCTCA TGACTGCCGA GCCAATGTCA TCGTGGTTGA CACACAGAAG 
CAGCTGGAAA AGATCCTGAA GATCTGGAAA GACTTGCCAC ATCTAAAGGC AGTGGTGATA 
TACCAAGAAC CCCCTCCAAA GAAGATGGCT AACGTGTACA CGATGGAGGA GCTCATAGAA 
CTGGGGCAAG AAGTGCCTGA GGAGGCCCTA GATGCCATCA TCGATACCCA GCAACCCAAC 
CAGTGCTGTG TGCTCGTCTA CACATCAGGC ACCACGGGAA ACCCCAAGGG TGTGATGCTG 
AGTCAAGACA ATATCACATG GACAGCACGG TATGGCAGTC AGGCTGGGGA CATCCAGCCA 
GCAGAAGTGC AGCAGGAGGT AGTGGTCAGC TACTTGCCTC TCAGCCACAT CGCTGCCCAG 
ATCTACGACC TGTGGACAGG CATCCAGTGG GGAGCCCAGG TCTGCTTTGC AGATCCGGAC 
GCCCTGAAGG GGACCCTGGT AAACACACTG CGGGAGGTGG AGCCCACATC CCACATGGGA 
GTGCCGCGTG TGTGGGAGAA GATCATGGAA AGGATCCAGG AGGTGGCCGC TCAGTCTGGC 
TTCATCCGCC GCAAGATGCT GCTGTGGGCC ATGTCAGTCA CCTTGGAACA GAACCTTACC 
TGCCCTAGCA ATGACCTGAA GCCCTTCACC AGCAGACTGG CGGATTACCT AGTGTTAGCC 
AGGGTCCGTC AGGCTCTGGG CTTTGCCAAG TGTCAGAAAA ACTTCTACGG AGCAGCTCCG 
ATGACGGCAG AGACGCAGCG CTTCTTCCTG GGCCTCAACA TCCGCCTGTA TGCAGGCTAT 
GGCCTCAGCG AGAGCACAGG CCCCCACTTC ATGTCCAGCC CCTACAACTA TCGGCTTTAC 
AGCTCCGGCA GGGTGGTACC CGGCTGCCGG GTGAAGCTGG TGAATCAGGA TGCAGATGGC 
ATCGGTGAGA TCTGCCTGTG GGGCCGCACC ATCTTCATGG GTTATCTGAA CATGGAGGAC 
AAAACCTGTG AGGCCATTGA TTCGGAAGGC TGGCTGCACA CAGGTGACAT GGGCCGCCTG 
GATGCTGACG GCTTCCTCTA CATCACCGGG CGCCTCAAAG AATTAATCAT CACTGCGGGC 
GGCGAGAACG TGCCCCCAGT GCCTATCGAG GAGGCCGTGA AGATGGAGCT GCCCATCATC 
AGCAGTGCCA TGCTGATCGG GGACCAGAGG AAGTTCCTGT CCATGCTGCT AACCCTGAAG 
TGCACGCTGG ACCCAGAGAC GTCTGAGCCT ACAGACAGCC TGACCGAGCA AGCTGTGGAG 
TTCTGCCAGC GGGTGGGCAG CAAGGCCAGC ACCGTCTCCG AGATCGTGGG GCAGAGAGAC 
GAGGCTGTGT ACCAGGCCAT TCACGAAGGG ATCCAGAGGG TGAACGCGAA CGCCGCAGCC 
CGGCCCTACC ACATCCAGAA GTGGGCCATT CTCCAACGTG ACTTCTCCAT TTCAGGTGGA 
GAACTGGGAC CCACCATGAA ATTGAAACGG CTCACGGTTC TGGAGAAGTA CAAAGATATC 
ATCGATTCTT TTTATCAAGA GCAAAAACAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acsbg1 ( NM_053178.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_444408.1 Mus musculus acyl-CoA synthetase bubblegum family member 1 (Acsbg1), mRNA.

Price & Availability↑

CloneID	OMu09301
Clone ID Related Accession (Same CDS sequence)	NM_053178.2
Accession Version	NM_053178.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	long-chain-fatty-acid--CoA ligase ACSBG1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC157591.2. On Mar 21, 2007 this sequence version replaced NM_053178.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB050554.1, SRR1660817.123614.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGCCACGCG GTTCTGAAGC AGGATACTGC TGTCTGTCCA GGGACTCCAA CATGCCTGAC 
AGCAGAGATG ATCAGCAGCA GGGGGCCAGC TTGGGGACCT CCCAAGACAA CTCGCAAACC 
AGCTCCTTAA TTGATGGGCA AACACTCTCC AAAGAGTCTC CAAGTCACGG TCTCGAGCTC 
TCAGCTCCAG AAAAGGCAAG GGCTGCCTCT TTGGATGGTG CAGAGGAAGC ACTATGGACA 
ACCCGGGCGG ATGGGAGAGT GCGCCTGCGC CTGGAACCTT TCTGTACTCA GCGTCCCTAT 
ACCGTGCACC AGATGTTCTA CGAGGCCCTA GATAAGTACG GGAACCTCAG TGCTCTGGGC 
TTCAAGCGCA AGGACAAGTG GGAGCGTATC TCTTACTACC AGTACTACCT GATTGCACGC 
AAAGTAGCCA AAGGCTTCTT GAAGCTCGGC CTAGAGCGTG CCCACAGCGT GGCGATCCTT 
GGCTTCAACT CTCCAGAATG GTTCTTCTCT GCAGTGGGCA CAGTGTTCGC AGGGGGCATT 
GTCACTGGCA TCTACACCAC CAGCTCCCCG GAGGCCTGCC AGTACATCTC TCATGACTGC 
CGAGCCAATG TCATCGTGGT TGACACACAG AAGCAGCTGG AAAAGATCCT GAAGATCTGG 
AAAGACTTGC CACATCTAAA GGCAGTGGTG ATATACCAAG AACCCCCTCC AAAGAAGATG 
GCTAACGTGT ACACGATGGA GGAGCTCATA GAACTGGGGC AAGAAGTGCC TGAGGAGGCC 
CTAGATGCCA TCATCGATAC CCAGCAACCC AACCAGTGCT GTGTGCTCGT CTACACATCA 
GGCACCACGG GAAACCCCAA GGGTGTGATG CTGAGTCAAG ACAATATCAC ATGGACAGCA 
CGGTATGGCA GTCAGGCTGG GGACATCCAG CCAGCAGAAG TGCAGCAGGA GGTAGTGGTC 
AGCTACTTGC CTCTCAGCCA CATCGCTGCC CAGATCTACG ACCTGTGGAC AGGCATCCAG 
TGGGGAGCCC AGGTCTGCTT TGCAGATCCG GACGCCCTGA AGGGGACCCT GGTAAACACA 
CTGCGGGAGG TGGAGCCCAC ATCCCACATG GGAGTGCCGC GTGTGTGGGA GAAGATCATG 
GAAAGGATCC AGGAGGTGGC CGCTCAGTCT GGCTTCATCC GCCGCAAGAT GCTGCTGTGG 
GCCATGTCAG TCACCTTGGA ACAGAACCTT ACCTGCCCTA GCAATGACCT GAAGCCCTTC 
ACCAGCAGAC TGGCGGATTA CCTAGTGTTA GCCAGGGTCC GTCAGGCTCT GGGCTTTGCC 
AAGTGTCAGA AAAACTTCTA CGGAGCAGCT CCGATGACGG CAGAGACGCA GCGCTTCTTC 
CTGGGCCTCA ACATCCGCCT GTATGCAGGC TATGGCCTCA GCGAGAGCAC AGGCCCCCAC 
TTCATGTCCA GCCCCTACAA CTATCGGCTT TACAGCTCCG GCAGGGTGGT ACCCGGCTGC 
CGGGTGAAGC TGGTGAATCA GGATGCAGAT GGCATCGGTG AGATCTGCCT GTGGGGCCGC 
ACCATCTTCA TGGGTTATCT GAACATGGAG GACAAAACCT GTGAGGCCAT TGATTCGGAA 
GGCTGGCTGC ACACAGGTGA CATGGGCCGC CTGGATGCTG ACGGCTTCCT CTACATCACC 
GGGCGCCTCA AAGAATTAAT CATCACTGCG GGCGGCGAGA ACGTGCCCCC AGTGCCTATC 
GAGGAGGCCG TGAAGATGGA GCTGCCCATC ATCAGCAGTG CCATGCTGAT CGGGGACCAG 
AGGAAGTTCC TGTCCATGCT GCTAACCCTG AAGTGCACGC TGGACCCAGA GACGTCTGAG 
CCTACAGACA GCCTGACCGA GCAAGCTGTG GAGTTCTGCC AGCGGGTGGG CAGCAAGGCC 
AGCACCGTCT CCGAGATCGT GGGGCAGAGA GACGAGGCTG TGTACCAGGC CATTCACGAA 
GGGATCCAGA GGGTGAACGC GAACGCCGCA GCCCGGCCCT ACCACATCCA GAAGTGGGCC 
ATTCTCCAAC GTGACTTCTC CATTTCAGGT GGAGAACTGG GACCCACCAT GAAATTGAAA 
CGGCTCACGG TTCTGGAGAA GTACAAAGAT ATCATCGATT CTTTTTATCA AGAGCAAAAA 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_444408.1
CDS	216..2381
Translation	MPRGSEAGYCCLSRDSNMPDSRDDQQQGASLGTSQDNSQTSSLIDGQTLSKESPSHGLELSAPEKARAASLDGAEEALWTTRADGRVRLRLEPFCTQRPYTVHQMFYEALDKYGNLSALGFKRKDKWERISYYQYYLIARKVAKGFLKLGLERAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYISHDCRANVIVVDTQKQLEKILKIWKDLPHLKAVVIYQEPPPKKMANVYTMEELIELGQEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFADPDALKGTLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIRRKMLLWAMSVTLEQNLTCPSNDLKPFTSRLADYLVLARVRQALGFAKCQKNFYGAAPMTAETQRFFLGLNIRLYAGYGLSESTGPHFMSSPYNYRLYSSGRVVPGCRVKLVNQDADGIGEICLWGRTIFMGYLNMEDKTCEAIDSEGWLHTGDMGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKMELPIISSAMLIGDQRKFLSMLLTLKCTLDPETSEPTDSLTEQAVEFCQRVGSKASTVSEIVGQRDEAVYQAIHEGIQRVNANAAARPYHIQKWAILQRDFSISGGELGPTMKLKRLTVLEKYKDIIDSFYQEQKQ

Target ORF information:

RefSeq Version	NM_053178.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus acyl-CoA synthetase bubblegum family member 1 (Acsbg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053178.2

ORF Insert Sequence:

ATGCCACGCG GTTCTGAAGC AGGATACTGC TGTCTGTCCA GGGACTCCAA CATGCCTGAC 
AGCAGAGATG ATCAGCAGCA GGGGGCCAGC TTGGGGACCT CCCAAGACAA CTCGCAAACC 
AGCTCCTTAA TTGATGGGCA AACACTCTCC AAAGAGTCTC CAAGTCACGG TCTCGAGCTC 
TCAGCTCCAG AAAAGGCAAG GGCTGCCTCT TTGGATGGTG CAGAGGAAGC ACTATGGACA 
ACCCGGGCGG ATGGGAGAGT GCGCCTGCGC CTGGAACCTT TCTGTACTCA GCGTCCCTAT 
ACCGTGCACC AGATGTTCTA CGAGGCCCTA GATAAGTACG GGAACCTCAG TGCTCTGGGC 
TTCAAGCGCA AGGACAAGTG GGAGCGTATC TCTTACTACC AGTACTACCT GATTGCACGC 
AAAGTAGCCA AAGGCTTCTT GAAGCTCGGC CTAGAGCGTG CCCACAGCGT GGCGATCCTT 
GGCTTCAACT CTCCAGAATG GTTCTTCTCT GCAGTGGGCA CAGTGTTCGC AGGGGGCATT 
GTCACTGGCA TCTACACCAC CAGCTCCCCG GAGGCCTGCC AGTACATCTC TCATGACTGC 
CGAGCCAATG TCATCGTGGT TGACACACAG AAGCAGCTGG AAAAGATCCT GAAGATCTGG 
AAAGACTTGC CACATCTAAA GGCAGTGGTG ATATACCAAG AACCCCCTCC AAAGAAGATG 
GCTAACGTGT ACACGATGGA GGAGCTCATA GAACTGGGGC AAGAAGTGCC TGAGGAGGCC 
CTAGATGCCA TCATCGATAC CCAGCAACCC AACCAGTGCT GTGTGCTCGT CTACACATCA 
GGCACCACGG GAAACCCCAA GGGTGTGATG CTGAGTCAAG ACAATATCAC ATGGACAGCA 
CGGTATGGCA GTCAGGCTGG GGACATCCAG CCAGCAGAAG TGCAGCAGGA GGTAGTGGTC 
AGCTACTTGC CTCTCAGCCA CATCGCTGCC CAGATCTACG ACCTGTGGAC AGGCATCCAG 
TGGGGAGCCC AGGTCTGCTT TGCAGATCCG GACGCCCTGA AGGGGACCCT GGTAAACACA 
CTGCGGGAGG TGGAGCCCAC ATCCCACATG GGAGTGCCGC GTGTGTGGGA GAAGATCATG 
GAAAGGATCC AGGAGGTGGC CGCTCAGTCT GGCTTCATCC GCCGCAAGAT GCTGCTGTGG 
GCCATGTCAG TCACCTTGGA ACAGAACCTT ACCTGCCCTA GCAATGACCT GAAGCCCTTC 
ACCAGCAGAC TGGCGGATTA CCTAGTGTTA GCCAGGGTCC GTCAGGCTCT GGGCTTTGCC 
AAGTGTCAGA AAAACTTCTA CGGAGCAGCT CCGATGACGG CAGAGACGCA GCGCTTCTTC 
CTGGGCCTCA ACATCCGCCT GTATGCAGGC TATGGCCTCA GCGAGAGCAC AGGCCCCCAC 
TTCATGTCCA GCCCCTACAA CTATCGGCTT TACAGCTCCG GCAGGGTGGT ACCCGGCTGC 
CGGGTGAAGC TGGTGAATCA GGATGCAGAT GGCATCGGTG AGATCTGCCT GTGGGGCCGC 
ACCATCTTCA TGGGTTATCT GAACATGGAG GACAAAACCT GTGAGGCCAT TGATTCGGAA 
GGCTGGCTGC ACACAGGTGA CATGGGCCGC CTGGATGCTG ACGGCTTCCT CTACATCACC 
GGGCGCCTCA AAGAATTAAT CATCACTGCG GGCGGCGAGA ACGTGCCCCC AGTGCCTATC 
GAGGAGGCCG TGAAGATGGA GCTGCCCATC ATCAGCAGTG CCATGCTGAT CGGGGACCAG 
AGGAAGTTCC TGTCCATGCT GCTAACCCTG AAGTGCACGC TGGACCCAGA GACGTCTGAG 
CCTACAGACA GCCTGACCGA GCAAGCTGTG GAGTTCTGCC AGCGGGTGGG CAGCAAGGCC 
AGCACCGTCT CCGAGATCGT GGGGCAGAGA GACGAGGCTG TGTACCAGGC CATTCACGAA 
GGGATCCAGA GGGTGAACGC GAACGCCGCA GCCCGGCCCT ACCACATCCA GAAGTGGGCC 
ATTCTCCAAC GTGACTTCTC CATTTCAGGT GGAGAACTGG GACCCACCAT GAAATTGAAA 
CGGCTCACGG TTCTGGAGAA GTACAAAGAT ATCATCGATT CTTTTTATCA AGAGCAAAAA 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acsbg1 ( XM_006511588.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511651.1 Mus musculus acyl-CoA synthetase bubblegum family member 1 (Acsbg1), transcript variant X1, mRNA.

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CloneID	OMu43075
Clone ID Related Accession (Same CDS sequence)	XM_006511588.3 , XM_006511588.2
Accession Version	XM_006511588.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	long-chain-fatty-acid--CoA ligase ACSBG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006511588.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGAAG TGCTTCTTCT CAGGTCTGCC AGCTCCATCC TGAGGACCGT GTTCGTAAGC 
AGACTGCTGC CAGGTGGGCC TGGGTGTGTT CGAAAACTTA GTTTGAACCT GAAGTACCAG 
CAAGGAATAA GGCCGAGTGT ACGAAGCAGC TCCTTAATTG ATGGGCAAAC ACTCTCCAAA 
GAGTCTCCAA GTCACGGTCT CGAGCTCTCA GCTCCAGAAA AGGCAAGGGC TGCCTCTTTG 
GATGGTGCAG AGGAAGCACT ATGGACAACC CGGGCGGATG GGAGAGTGCG CCTGCGCCTG 
GAACCTTTCT GTACTCAGCG TCCCTATACC GTGCACCAGA TGTTCTACGA GGCCCTAGAT 
AAGTACGGGA ACCTCAGTGC TCTGGGCTTC AAGCGCAAGG ACAAGTGGGA GCGTATCTCT 
TACTACCAGT ACTACCTGAT TGCACGCAAA GTAGCCAAAG GCTTCTTGAA GCTCGGCCTA 
GAGCGTGCCC ACAGCGTGGC GATCCTTGGC TTCAACTCTC CAGAATGGTT CTTCTCTGCA 
GTGGGCACAG TGTTCGCAGG GGGCATTGTC ACTGGCATCT ACACCACCAG CTCCCCGGAG 
GCCTGCCAGT ACATCTCTCA TGACTGCCGA GCCAATGTCA TCGTGGTTGA CACACAGAAG 
CAGCTGGAAA AGATCCTGAA GATCTGGAAA GACTTGCCAC ATCTAAAGGC AGTGGTGATA 
TACCAAGAAC CCCCTCCAAA GAAGATGGCT AACGTGTACA CGATGGAGGA GCTCATAGAA 
CTGGGGCAAG AAGTGCCTGA GGAGGCCCTA GATGCCATCA TCGATACCCA GCAACCCAAC 
CAGTGCTGTG TGCTCGTCTA CACATCAGGC ACCACGGGAA ACCCCAAGGG TGTGATGCTG 
AGTCAAGACA ATATCACATG GACAGCACGG TATGGCAGTC AGGCTGGGGA CATCCAGCCA 
GCAGAAGTGC AGCAGGAGGT AGTGGTCAGC TACTTGCCTC TCAGCCACAT CGCTGCCCAG 
ATCTACGACC TGTGGACAGG CATCCAGTGG GGAGCCCAGG TCTGCTTTGC AGATCCGGAC 
GCCCTGAAGG GGACCCTGGT AAACACACTG CGGGAGGTGG AGCCCACATC CCACATGGGA 
GTGCCGCGTG TGTGGGAGAA GATCATGGAA AGGATCCAGG AGGTGGCCGC TCAGTCTGGC 
TTCATCCGCC GCAAGATGCT GCTGTGGGCC ATGTCAGTCA CCTTGGAACA GAACCTTACC 
TGCCCTAGCA ATGACCTGAA GCCCTTCACC AGCAGACTGG CGGATTACCT AGTGTTAGCC 
AGGGTCCGTC AGGCTCTGGG CTTTGCCAAG TGTCAGAAAA ACTTCTACGG AGCAGCTCCG 
ATGACGGCAG AGACGCAGCG CTTCTTCCTG GGCCTCAACA TCCGCCTGTA TGCAGGCTAT 
GGCCTCAGCG AGAGCACAGG CCCCCACTTC ATGTCCAGCC CCTACAACTA TCGGCTTTAC 
AGCTCCGGCA GGGTGGTACC CGGCTGCCGG GTGAAGCTGG TGAATCAGGA TGCAGATGGC 
ATCGGTGAGA TCTGCCTGTG GGGCCGCACC ATCTTCATGG GTTATCTGAA CATGGAGGAC 
AAAACCTGTG AGGCCATTGA TTCGGAAGGC TGGCTGCACA CAGGTGACAT GGGCCGCCTG 
GATGCTGACG GCTTCCTCTA CATCACCGGG CGCCTCAAAG AATTAATCAT CACTGCGGGC 
GGCGAGAACG TGCCCCCAGT GCCTATCGAG GAGGCCGTGA AGATGGAGCT GCCCATCATC 
AGCAGTGCCA TGCTGATCGG GGACCAGAGG AAGTTCCTGT CCATGCTGCT AACCCTGAAG 
TGCACGCTGG ACCCAGAGAC GTCTGAGCCT ACAGACAGCC TGACCGAGCA AGCTGTGGAG 
TTCTGCCAGC GGGTGGGCAG CAAGGCCAGC ACCGTCTCCG AGATCGTGGG GCAGAGAGAC 
GAGGCTGTGT ACCAGGCCAT TCACGAAGGG ATCCAGAGGG TGAACGCGAA CGCCGCAGCC 
CGGCCCTACC ACATCCAGAA GTGGGCCATT CTCCAACGTG ACTTCTCCAT TTCAGGTGGA 
GAACTGGGAC CCACCATGAA ATTGAAACGG CTCACGGTTC TGGAGAAGTA CAAAGATATC 
ATCGATTCTT TTTATCAAGA GCAAAAACAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511651.1
CDS	334..2526
Translation	MPEVLLLRSASSILRTVFVSRLLPGGPGCVRKLSLNLKYQQGIRPSVRSSSLIDGQTLSKESPSHGLELSAPEKARAASLDGAEEALWTTRADGRVRLRLEPFCTQRPYTVHQMFYEALDKYGNLSALGFKRKDKWERISYYQYYLIARKVAKGFLKLGLERAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYISHDCRANVIVVDTQKQLEKILKIWKDLPHLKAVVIYQEPPPKKMANVYTMEELIELGQEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFADPDALKGTLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIRRKMLLWAMSVTLEQNLTCPSNDLKPFTSRLADYLVLARVRQALGFAKCQKNFYGAAPMTAETQRFFLGLNIRLYAGYGLSESTGPHFMSSPYNYRLYSSGRVVPGCRVKLVNQDADGIGEICLWGRTIFMGYLNMEDKTCEAIDSEGWLHTGDMGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKMELPIISSAMLIGDQRKFLSMLLTLKCTLDPETSEPTDSLTEQAVEFCQRVGSKASTVSEIVGQRDEAVYQAIHEGIQRVNANAAARPYHIQKWAILQRDFSISGGELGPTMKLKRLTVLEKYKDIIDSFYQEQKQ

Target ORF information:

RefSeq Version	XM_006511588.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus acyl-CoA synthetase bubblegum family member 1 (Acsbg1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511588.3

ORF Insert Sequence:

ATGCCAGAAG TGCTTCTTCT CAGGTCTGCC AGCTCCATCC TGAGGACCGT GTTCGTAAGC 
AGACTGCTGC CAGGTGGGCC TGGGTGTGTT CGAAAACTTA GTTTGAACCT GAAGTACCAG 
CAAGGAATAA GGCCGAGTGT ACGAAGCAGC TCCTTAATTG ATGGGCAAAC ACTCTCCAAA 
GAGTCTCCAA GTCACGGTCT CGAGCTCTCA GCTCCAGAAA AGGCAAGGGC TGCCTCTTTG 
GATGGTGCAG AGGAAGCACT ATGGACAACC CGGGCGGATG GGAGAGTGCG CCTGCGCCTG 
GAACCTTTCT GTACTCAGCG TCCCTATACC GTGCACCAGA TGTTCTACGA GGCCCTAGAT 
AAGTACGGGA ACCTCAGTGC TCTGGGCTTC AAGCGCAAGG ACAAGTGGGA GCGTATCTCT 
TACTACCAGT ACTACCTGAT TGCACGCAAA GTAGCCAAAG GCTTCTTGAA GCTCGGCCTA 
GAGCGTGCCC ACAGCGTGGC GATCCTTGGC TTCAACTCTC CAGAATGGTT CTTCTCTGCA 
GTGGGCACAG TGTTCGCAGG GGGCATTGTC ACTGGCATCT ACACCACCAG CTCCCCGGAG 
GCCTGCCAGT ACATCTCTCA TGACTGCCGA GCCAATGTCA TCGTGGTTGA CACACAGAAG 
CAGCTGGAAA AGATCCTGAA GATCTGGAAA GACTTGCCAC ATCTAAAGGC AGTGGTGATA 
TACCAAGAAC CCCCTCCAAA GAAGATGGCT AACGTGTACA CGATGGAGGA GCTCATAGAA 
CTGGGGCAAG AAGTGCCTGA GGAGGCCCTA GATGCCATCA TCGATACCCA GCAACCCAAC 
CAGTGCTGTG TGCTCGTCTA CACATCAGGC ACCACGGGAA ACCCCAAGGG TGTGATGCTG 
AGTCAAGACA ATATCACATG GACAGCACGG TATGGCAGTC AGGCTGGGGA CATCCAGCCA 
GCAGAAGTGC AGCAGGAGGT AGTGGTCAGC TACTTGCCTC TCAGCCACAT CGCTGCCCAG 
ATCTACGACC TGTGGACAGG CATCCAGTGG GGAGCCCAGG TCTGCTTTGC AGATCCGGAC 
GCCCTGAAGG GGACCCTGGT AAACACACTG CGGGAGGTGG AGCCCACATC CCACATGGGA 
GTGCCGCGTG TGTGGGAGAA GATCATGGAA AGGATCCAGG AGGTGGCCGC TCAGTCTGGC 
TTCATCCGCC GCAAGATGCT GCTGTGGGCC ATGTCAGTCA CCTTGGAACA GAACCTTACC 
TGCCCTAGCA ATGACCTGAA GCCCTTCACC AGCAGACTGG CGGATTACCT AGTGTTAGCC 
AGGGTCCGTC AGGCTCTGGG CTTTGCCAAG TGTCAGAAAA ACTTCTACGG AGCAGCTCCG 
ATGACGGCAG AGACGCAGCG CTTCTTCCTG GGCCTCAACA TCCGCCTGTA TGCAGGCTAT 
GGCCTCAGCG AGAGCACAGG CCCCCACTTC ATGTCCAGCC CCTACAACTA TCGGCTTTAC 
AGCTCCGGCA GGGTGGTACC CGGCTGCCGG GTGAAGCTGG TGAATCAGGA TGCAGATGGC 
ATCGGTGAGA TCTGCCTGTG GGGCCGCACC ATCTTCATGG GTTATCTGAA CATGGAGGAC 
AAAACCTGTG AGGCCATTGA TTCGGAAGGC TGGCTGCACA CAGGTGACAT GGGCCGCCTG 
GATGCTGACG GCTTCCTCTA CATCACCGGG CGCCTCAAAG AATTAATCAT CACTGCGGGC 
GGCGAGAACG TGCCCCCAGT GCCTATCGAG GAGGCCGTGA AGATGGAGCT GCCCATCATC 
AGCAGTGCCA TGCTGATCGG GGACCAGAGG AAGTTCCTGT CCATGCTGCT AACCCTGAAG 
TGCACGCTGG ACCCAGAGAC GTCTGAGCCT ACAGACAGCC TGACCGAGCA AGCTGTGGAG 
TTCTGCCAGC GGGTGGGCAG CAAGGCCAGC ACCGTCTCCG AGATCGTGGG GCAGAGAGAC 
GAGGCTGTGT ACCAGGCCAT TCACGAAGGG ATCCAGAGGG TGAACGCGAA CGCCGCAGCC 
CGGCCCTACC ACATCCAGAA GTGGGCCATT CTCCAACGTG ACTTCTCCAT TTCAGGTGGA 
GAACTGGGAC CCACCATGAA ATTGAAACGG CTCACGGTTC TGGAGAAGTA CAAAGATATC 
ATCGATTCTT TTTATCAAGA GCAAAAACAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acsbg1 ( XM_030244753.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100613.1 Mus musculus acyl-CoA synthetase bubblegum family member 1 (Acsbg1), transcript variant X2, mRNA.

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CloneID	OMu43076
Clone ID Related Accession (Same CDS sequence)	XM_030244753.1
Accession Version	XM_030244753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	long-chain-fatty-acid--CoA ligase ACSBG1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCG GTTCTGAAGC AGGATACTGC TGTCTGTCCA GGGACTCCAA CATGCCTGAC 
AGCAGAGATG ATCAGCAGCA GGGGGCCAGC TTGGGGACCT CCCAAGACAA CTCGCAAACC 
AGGTTCGTAA GCAGACTGCT GCCAGGTGGG CCTGGGTGTG TTCGAAAACT TAGTTTGAAC 
CTGAAGTACC AGCAAGGAAT AAGGCCGAGT GTACGAAGCA GCTCCTTAAT TGATGGGCAA 
ACACTCTCCA AAGAGTCTCC AAGTCACGGT CTCGAGCTCT CAGCTCCAGA AAAGGCAAGG 
GCTGCCTCTT TGGATGGTGC AGAGGAAGCA CTATGGACAA CCCGGGCGGA TGGGAGAGTG 
CGCCTGCGCC TGGAACCTTT CTGTACTCAG CGTCCCTATA CCGTGCACCA GATGTTCTAC 
GAGGCCCTAG ATAAGTACGG GAACCTCAGT GCTCTGGGCT TCAAGCGCAA GGACAAGTGG 
GAGCGTATCT CTTACTACCA GTACTACCTG ATTGCACGCA AAGTAGCCAA AGGCTTCTTG 
AAGCTCGGCC TAGAGCGTGC CCACAGCGTG GCGATCCTTG GCTTCAACTC TCCAGAATGG 
TTCTTCTCTG CAGTGGGCAC AGTGTTCGCA GGGGGCATTG TCACTGGCAT CTACACCACC 
AGCTCCCCGG AGGCCTGCCA GTACATCTCT CATGACTGCC GAGCCAATGT CATCGTGGTT 
GACACACAGA AGCAGCTGGA AAAGATCCTG AAGATCTGGA AAGACTTGCC ACATCTAAAG 
GCAGTGGTGA TATACCAAGA ACCCCCTCCA AAGAAGATGG CTAACGTGTA CACGATGGAG 
GAGCTCATAG AACTGGGGCA AGAAGTGCCT GAGGAGGCCC TAGATGCCAT CATCGATACC 
CAGCAACCCA ACCAGTGCTG TGTGCTCGTC TACACATCAG GCACCACGGG AAACCCCAAG 
GGTGTGATGC TGAGTCAAGA CAATATCACA TGGACAGCAC GGTATGGCAG TCAGGCTGGG 
GACATCCAGC CAGCAGAAGT GCAGCAGGAG GTAGTGGTCA GCTACTTGCC TCTCAGCCAC 
ATCGCTGCCC AGATCTACGA CCTGTGGACA GGCATCCAGT GGGGAGCCCA GGTCTGCTTT 
GCAGATCCGG ACGCCCTGAA GGGGACCCTG GTAAACACAC TGCGGGAGGT GGAGCCCACA 
TCCCACATGG GAGTGCCGCG TGTGTGGGAG AAGATCATGG AAAGGATCCA GGAGGTGGCC 
GCTCAGTCTG GCTTCATCCG CCGCAAGATG CTGCTGTGGG CCATGTCAGT CACCTTGGAA 
CAGAACCTTA CCTGCCCTAG CAATGACCTG AAGCCCTTCA CCAGCAGACT GGCGGATTAC 
CTAGTGTTAG CCAGGGTCCG TCAGGCTCTG GGCTTTGCCA AGTGTCAGAA AAACTTCTAC 
GGAGCAGCTC CGATGACGGC AGAGACGCAG CGCTTCTTCC TGGGCCTCAA CATCCGCCTG 
TATGCAGGCT ATGGCCTCAG CGAGAGCACA GGCCCCCACT TCATGTCCAG CCCCTACAAC 
TATCGGCTTT ACAGCTCCGG CAGGGTGGTA CCCGGCTGCC GGGTGAAGCT GGTGAATCAG 
GATGCAGATG GCATCGGTGA GATCTGCCTG TGGGGCCGCA CCATCTTCAT GGGTTATCTG 
AACATGGAGG ACAAAACCTG TGAGGCCATT GATTCGGAAG GCTGGCTGCA CACAGGTGAC 
ATGGGCCGCC TGGATGCTGA CGGCTTCCTC TACATCACCG GGCGCCTCAA AGAATTAATC 
ATCACTGCGG GCGGCGAGAA CGTGCCCCCA GTGCCTATCG AGGAGGCCGT GAAGATGGAG 
CTGCCCATCA TCAGCAGTGC CATGCTGATC GGGGACCAGA GGAAGTTCCT GTCCATGCTG 
CTAACCCTGA AGTGCACGCT GGACCCAGAG ACGTCTGAGC CTACAGACAG CCTGACCGAG 
CAAGCTGTGG AGTTCTGCCA GCGGGTGGGC AGCAAGGCCA GCACCGTCTC CGAGATCGTG 
GGGCAGAGAG ACGAGGCTGT GTACCAGGCC ATTCACGAAG GGATCCAGAG GGTGAACGCG 
AACGCCGCAG CCCGGCCCTA CCACATCCAG AAGTGGGCCA TTCTCCAACG TGACTTCTCC 
ATTTCAGGTG GAGAACTGGG ACCCACCATG AAATTGAAAC GGCTCACGGT TCTGGAGAAG 
TACAAAGATA TCATCGATTC TTTTTATCAA GAGCAAAAAC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100613.1
CDS	96..2360
Translation	MPRGSEAGYCCLSRDSNMPDSRDDQQQGASLGTSQDNSQTRFVSRLLPGGPGCVRKLSLNLKYQQGIRPSVRSSSLIDGQTLSKESPSHGLELSAPEKARAASLDGAEEALWTTRADGRVRLRLEPFCTQRPYTVHQMFYEALDKYGNLSALGFKRKDKWERISYYQYYLIARKVAKGFLKLGLERAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYISHDCRANVIVVDTQKQLEKILKIWKDLPHLKAVVIYQEPPPKKMANVYTMEELIELGQEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFADPDALKGTLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIRRKMLLWAMSVTLEQNLTCPSNDLKPFTSRLADYLVLARVRQALGFAKCQKNFYGAAPMTAETQRFFLGLNIRLYAGYGLSESTGPHFMSSPYNYRLYSSGRVVPGCRVKLVNQDADGIGEICLWGRTIFMGYLNMEDKTCEAIDSEGWLHTGDMGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKMELPIISSAMLIGDQRKFLSMLLTLKCTLDPETSEPTDSLTEQAVEFCQRVGSKASTVSEIVGQRDEAVYQAIHEGIQRVNANAAARPYHIQKWAILQRDFSISGGELGPTMKLKRLTVLEKYKDIIDSFYQEQKQ

Target ORF information:

RefSeq Version	XM_030244753.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus acyl-CoA synthetase bubblegum family member 1 (Acsbg1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244753.1

ORF Insert Sequence:

ATGCCACGCG GTTCTGAAGC AGGATACTGC TGTCTGTCCA GGGACTCCAA CATGCCTGAC 
AGCAGAGATG ATCAGCAGCA GGGGGCCAGC TTGGGGACCT CCCAAGACAA CTCGCAAACC 
AGGTTCGTAA GCAGACTGCT GCCAGGTGGG CCTGGGTGTG TTCGAAAACT TAGTTTGAAC 
CTGAAGTACC AGCAAGGAAT AAGGCCGAGT GTACGAAGCA GCTCCTTAAT TGATGGGCAA 
ACACTCTCCA AAGAGTCTCC AAGTCACGGT CTCGAGCTCT CAGCTCCAGA AAAGGCAAGG 
GCTGCCTCTT TGGATGGTGC AGAGGAAGCA CTATGGACAA CCCGGGCGGA TGGGAGAGTG 
CGCCTGCGCC TGGAACCTTT CTGTACTCAG CGTCCCTATA CCGTGCACCA GATGTTCTAC 
GAGGCCCTAG ATAAGTACGG GAACCTCAGT GCTCTGGGCT TCAAGCGCAA GGACAAGTGG 
GAGCGTATCT CTTACTACCA GTACTACCTG ATTGCACGCA AAGTAGCCAA AGGCTTCTTG 
AAGCTCGGCC TAGAGCGTGC CCACAGCGTG GCGATCCTTG GCTTCAACTC TCCAGAATGG 
TTCTTCTCTG CAGTGGGCAC AGTGTTCGCA GGGGGCATTG TCACTGGCAT CTACACCACC 
AGCTCCCCGG AGGCCTGCCA GTACATCTCT CATGACTGCC GAGCCAATGT CATCGTGGTT 
GACACACAGA AGCAGCTGGA AAAGATCCTG AAGATCTGGA AAGACTTGCC ACATCTAAAG 
GCAGTGGTGA TATACCAAGA ACCCCCTCCA AAGAAGATGG CTAACGTGTA CACGATGGAG 
GAGCTCATAG AACTGGGGCA AGAAGTGCCT GAGGAGGCCC TAGATGCCAT CATCGATACC 
CAGCAACCCA ACCAGTGCTG TGTGCTCGTC TACACATCAG GCACCACGGG AAACCCCAAG 
GGTGTGATGC TGAGTCAAGA CAATATCACA TGGACAGCAC GGTATGGCAG TCAGGCTGGG 
GACATCCAGC CAGCAGAAGT GCAGCAGGAG GTAGTGGTCA GCTACTTGCC TCTCAGCCAC 
ATCGCTGCCC AGATCTACGA CCTGTGGACA GGCATCCAGT GGGGAGCCCA GGTCTGCTTT 
GCAGATCCGG ACGCCCTGAA GGGGACCCTG GTAAACACAC TGCGGGAGGT GGAGCCCACA 
TCCCACATGG GAGTGCCGCG TGTGTGGGAG AAGATCATGG AAAGGATCCA GGAGGTGGCC 
GCTCAGTCTG GCTTCATCCG CCGCAAGATG CTGCTGTGGG CCATGTCAGT CACCTTGGAA 
CAGAACCTTA CCTGCCCTAG CAATGACCTG AAGCCCTTCA CCAGCAGACT GGCGGATTAC 
CTAGTGTTAG CCAGGGTCCG TCAGGCTCTG GGCTTTGCCA AGTGTCAGAA AAACTTCTAC 
GGAGCAGCTC CGATGACGGC AGAGACGCAG CGCTTCTTCC TGGGCCTCAA CATCCGCCTG 
TATGCAGGCT ATGGCCTCAG CGAGAGCACA GGCCCCCACT TCATGTCCAG CCCCTACAAC 
TATCGGCTTT ACAGCTCCGG CAGGGTGGTA CCCGGCTGCC GGGTGAAGCT GGTGAATCAG 
GATGCAGATG GCATCGGTGA GATCTGCCTG TGGGGCCGCA CCATCTTCAT GGGTTATCTG 
AACATGGAGG ACAAAACCTG TGAGGCCATT GATTCGGAAG GCTGGCTGCA CACAGGTGAC 
ATGGGCCGCC TGGATGCTGA CGGCTTCCTC TACATCACCG GGCGCCTCAA AGAATTAATC 
ATCACTGCGG GCGGCGAGAA CGTGCCCCCA GTGCCTATCG AGGAGGCCGT GAAGATGGAG 
CTGCCCATCA TCAGCAGTGC CATGCTGATC GGGGACCAGA GGAAGTTCCT GTCCATGCTG 
CTAACCCTGA AGTGCACGCT GGACCCAGAG ACGTCTGAGC CTACAGACAG CCTGACCGAG 
CAAGCTGTGG AGTTCTGCCA GCGGGTGGGC AGCAAGGCCA GCACCGTCTC CGAGATCGTG 
GGGCAGAGAG ACGAGGCTGT GTACCAGGCC ATTCACGAAG GGATCCAGAG GGTGAACGCG 
AACGCCGCAG CCCGGCCCTA CCACATCCAG AAGTGGGCCA TTCTCCAACG TGACTTCTCC 
ATTTCAGGTG GAGAACTGGG ACCCACCATG AAATTGAAAC GGCTCACGGT TCTGGAGAAG 
TACAAAGATA TCATCGATTC TTTTTATCAA GAGCAAAAAC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNF20 controls astrocytic differentiation through epigenetic regulation of STAT3 in the developing brain.
Cell death and differentiation25(2)294-306(2018 Feb)
Liang Q,Xia W,Li W,Jiao J

Analogous mechanism regulating formation of neocortical basal radial glia and cerebellar Bergmann glia.
eLife6(2017 May)
Heng X,Guo Q,Leung AW,Li JY

DISC1 regulates astrogenesis in the embryonic brain via modulation of RAS/MEK/ERK signaling through RASSF7.
Development (Cambridge, England)143(15)2732-40(2016 08)
Wang S,Liang Q,Qiao H,Li H,Shen T,Ji F,Jiao J

Conserved molecular signatures of neurogenesis in the hippocampal subgranular zone of rodents and primates.
Development (Cambridge, England)140(22)4633-44(2013 Nov)
Miller JA,Nathanson J,Franjic D,Shim S,Dalley RA,Shapouri S,Smith KA,Sunkin SM,Bernard A,Bennett JL,Lee CK,Hawrylycz MJ,Jones AR,Amaral DG,?estan N,Gage FH,Lein ES

MEK Is a Key Regulator of Gliogenesis in the Developing Brain.
Neuron75(6)1035-50(2012 Sep)
Li X,Newbern JM,Wu Y,Morgan-Smith M,Zhong J,Charron J,Snider WD

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Acsbg1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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