Amn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Amn
Entrez Gene ID	93835
Full Name	amnionless
General protein information	Preferred Names amnionless Names protein amnionless type I transmembrane protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12 F1\|12 60.94 cM
Summary	This gene encodes a type I transmembrane protein. The encoded protein is an essential component of the cubulin receptor complex which is thought to play a role in coordinating growth and patterning of the embryo. This protein is thought to modulate a bone morphogenetic protein (BMP) signaling pathway. A homoygous mutation in the mouse gene results in the lack of an amnion in embryos. Mutations in the human gene are associated with Megaloblastic Anemia-1. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_030247005.1	XP_030102865.1	protein amnionless isoform X1
NM_033603.3	NP_291081.2	protein amnionless precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	AMN	NP_001070509.1
Gallus gallus (chicken)	AMN	NP_001264445.1
Danio rerio (zebrafish)	amn	XP_002661318.2
Xenopus tropicalis (tropical clawed frog)	amn	XP_002938043.1
Homo sapiens (human)	AMN	NP_112205.2
Mus musculus (house mouse)	Amn	NP_291081.2
Pan troglodytes (chimpanzee)	AMN	XP_510179.2
Rattus norvegicus (Norway rat)	Amn	NP_001101531.1

Gene Ontology
Bibliography

Related articles in PubMed

Imprinted expression in cystic embryoid bodies shows an embryonic and not an extra-embryonic pattern.
Kulinski TM, Casari MR, Guenzl PM, Wenzel D, Andergassen D, Hladik A, Datlinger P, Farlik M, Theussl HC, Penninger JM, Knapp S, Bock C, Barlow DP, Hudson QJ
Developmental biology402(2)291-305(2015 Jun)

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Li Y, Klena NT, Gabriel GC, Liu X, Kim AJ, Lemke K, Chen Y, Chatterjee B, Devine W, Damerla RR, Chang C, Yagi H, San Agustin JT, Thahir M, Anderton S, Lawhead C, Vescovi A, Pratt H, Morgan J, Haynes L, Smith CL, Eppig JT, Reinholdt L, Francis R, Leatherbury L, Ganapathiraju MK, Tobita K, Pazour GJ, Lo CW
Nature521(7553)520-4(2015 May)

The novel helicase helG (DHX30) is expressed during gastrulation in mice and has a structure similar to a human DExH box helicase.
Zheng HJ, Tsukahara M, Liu E, Ye L, Xiong H, Noguchi S, Suzuki K, Ji ZS
Stem cells and development24(3)372-83(2015 Feb)

Expression of amnionless in mouse testes and Leydig cells.
Oh YS, Park HY, Gye MC
Andrologia44 Suppl 1383-9(2012 May)

O-fucosylation of thrombospondin type 1 repeats restricts epithelial to mesenchymal transition (EMT) and maintains epiblast pluripotency during mouse gastrulation.
Du J, Takeuchi H, Leonhard-Melief C, Shroyer KR, Dlugosz M, Haltiwanger RS, Holdener BC
Developmental biology346(1)25-38(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

AMN may participate in meiosis in early spermatocytes and in functional differentiation of adult Leydig cells through the mediation of vitamin B(12) transport in the mouse testes
Title: Expression of amnionless in mouse testes and Leydig cells.

Amn is an essential component of the Cubn receptor complex
Title: Mouse amnionless, which is required for primitive streak assembly, mediates cell-surface localization and endocytic function of cubilin on visceral endoderm and kidney proximal tubules.

The following Amn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Amn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08871	NM_033603.3 Latest version!	Mus musculus amnionless (Amn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu86175	XM_030247005.1 Latest version!	Mus musculus amnionless (Amn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Amn ( NM_033603.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_291081.2 Mus musculus amnionless (Amn), mRNA.

Price & Availability↑

CloneID	OMu08871
Clone ID Related Accession (Same CDS sequence)	NM_033603.3
Accession Version	NM_033603.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Mus musculus(house mouse)
Product	protein amnionless precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB873437.1, BC087954.1 and AC127580.3. On Sep 17, 2015 this sequence version replaced NM_033603.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF320619.1, AK017204.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTGCTC TGGGCCGGGT CCTGCTGTGG CTGCAGCTCT GTGCAATGAC TCGGGCCGCC 
TACAAACTTT GGGTCCCCAA TACCTCCTTC GATACTGCCT CCAACTGGAA CCAGAACCGG 
ACCCCGTGCG CGGGAGATGC GGTTCAGTTC CCGGCGGACA AGATGGTGTC GGTCCTGGTA 
CGAGACAGTC ACGCCATCTC GGATATGCTC CTGCCTCTGG ACGGGGAGTT GGTCCTGGCC 
TCGGGAGCTG CACTCAGCGC TGCAGGAGGC GACTCGGACC CAGCTTGTAA CCCTGGCGCC 
CCGTTGCTCT TCCGCAATCC CGATCGCTTC TCCTGGCTTG ACCCGCACCT CTGGAGCTCC 
GGGACTCAGG CGCCCGGGCT CTTCTCCGTG GACGCCGAGC GCGTGCCCTG CAGCTACGAC 
GACGTCCTCT TCCCGCGCGA TGGATCTTTC CGCGTGGCGC TTGGCCCAGG CCCCAACCCG 
GTGCACGTTC GCAGCGTCTC GGCAGTGGGC CAGACGTTCT CGCGCGACGA GGACCTGACT 
GCTTTCCTGG CGTCCCGCGA GGGCCGCCTG CGCTTCCACG GGTCGGGCGC GCTGAGAGTG 
GGGTCCCAAG CTTGCACCGA CGCGTCGGGC TGCGTCTGTG GCAACGCCGA GATGCTGCCG 
TGGATCTGCG CGTCTCTGCT GCAGCCCTTG GGCGGCCGCT GTCCCCAGGC CGCCTGCCAG 
GACCCCCTAC TGCCTCAAGG ACAATGCTGT GACCTCTGCG GAGCTATCGT GTCGTTGACC 
CACGACCCCA CATTTGACCT GGAGCGGTAC CGAGCGCGGC TGCTGGACCT CTTCCTGAAG 
CAGCCCCAGT ACCAGGGCCT GCAGGTGGCT GTGTCCAAGG TGCTGCGCGA CGCCCACACC 
GAGATCCAGG TGGTGCTGGT GGAGACCGAG CACGCGACCG GCGCGGCGGG GCAGCTGGGC 
CACGCGCTCC TCCAGGACGC GGTGGCACAA GGCAGCGTGC TCGGTATCGT GTCGGCGACC 
CTGCGGCAGT CCGGCAAGCC TATGACGGCA GACTCAGAGC TGAATCAGAG CTCGTCCGGT 
GCCGGGCTGG CGGGCGGCGT GGCGGCCTTG GTGCTGCTGG CTCTGCTGGG GACCGTGCTC 
CTTCTGCTGC ACCGCAGCGG AAGGCTCAGG TGGAGAAGGC ATGAAGACGC TGAGCCCGTA 
TCGGCTGGGC TGCCGCTGGG TTTCCGAAAT CCGATTTTCG ACGCGATTGT CTTCAAGCAG 
CAGCCTTCGG TCGAGCTACC TGACTCAGCT CAGAAGGTGG ACATCTTGGA CATTGATACC 
AAGTTCGGCT GCTTCGTTAA CCCACTCTTT GCTGGGGAGG CAGAGGCAGA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_291081.2
CDS	27..1403
Translation	MGALGRVLLWLQLCAMTRAAYKLWVPNTSFDTASNWNQNRTPCAGDAVQFPADKMVSVLVRDSHAISDMLLPLDGELVLASGAALSAAGGDSDPACNPGAPLLFRNPDRFSWLDPHLWSSGTQAPGLFSVDAERVPCSYDDVLFPRDGSFRVALGPGPNPVHVRSVSAVGQTFSRDEDLTAFLASREGRLRFHGSGALRVGSQACTDASGCVCGNAEMLPWICASLLQPLGGRCPQAACQDPLLPQGQCCDLCGAIVSLTHDPTFDLERYRARLLDLFLKQPQYQGLQVAVSKVLRDAHTEIQVVLVETEHATGAAGQLGHALLQDAVAQGSVLGIVSATLRQSGKPMTADSELNQSSSGAGLAGGVAALVLLALLGTVLLLLHRSGRLRWRRHEDAEPVSAGLPLGFRNPIFDAIVFKQQPSVELPDSAQKVDILDIDTKFGCFVNPLFAGEAEAEA

Target ORF information:

RefSeq Version	NM_033603.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus amnionless (Amn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033603.3

ORF Insert Sequence:

ATGGGTGCTC TGGGCCGGGT CCTGCTGTGG CTGCAGCTCT GTGCAATGAC TCGGGCCGCC 
TACAAACTTT GGGTCCCCAA TACCTCCTTC GATACTGCCT CCAACTGGAA CCAGAACCGG 
ACCCCGTGCG CGGGAGATGC GGTTCAGTTC CCGGCGGACA AGATGGTGTC GGTCCTGGTA 
CGAGACAGTC ACGCCATCTC GGATATGCTC CTGCCTCTGG ACGGGGAGTT GGTCCTGGCC 
TCGGGAGCTG CACTCAGCGC TGCAGGAGGC GACTCGGACC CAGCTTGTAA CCCTGGCGCC 
CCGTTGCTCT TCCGCAATCC CGATCGCTTC TCCTGGCTTG ACCCGCACCT CTGGAGCTCC 
GGGACTCAGG CGCCCGGGCT CTTCTCCGTG GACGCCGAGC GCGTGCCCTG CAGCTACGAC 
GACGTCCTCT TCCCGCGCGA TGGATCTTTC CGCGTGGCGC TTGGCCCAGG CCCCAACCCG 
GTGCACGTTC GCAGCGTCTC GGCAGTGGGC CAGACGTTCT CGCGCGACGA GGACCTGACT 
GCTTTCCTGG CGTCCCGCGA GGGCCGCCTG CGCTTCCACG GGTCGGGCGC GCTGAGAGTG 
GGGTCCCAAG CTTGCACCGA CGCGTCGGGC TGCGTCTGTG GCAACGCCGA GATGCTGCCG 
TGGATCTGCG CGTCTCTGCT GCAGCCCTTG GGCGGCCGCT GTCCCCAGGC CGCCTGCCAG 
GACCCCCTAC TGCCTCAAGG ACAATGCTGT GACCTCTGCG GAGCTATCGT GTCGTTGACC 
CACGACCCCA CATTTGACCT GGAGCGGTAC CGAGCGCGGC TGCTGGACCT CTTCCTGAAG 
CAGCCCCAGT ACCAGGGCCT GCAGGTGGCT GTGTCCAAGG TGCTGCGCGA CGCCCACACC 
GAGATCCAGG TGGTGCTGGT GGAGACCGAG CACGCGACCG GCGCGGCGGG GCAGCTGGGC 
CACGCGCTCC TCCAGGACGC GGTGGCACAA GGCAGCGTGC TCGGTATCGT GTCGGCGACC 
CTGCGGCAGT CCGGCAAGCC TATGACGGCA GACTCAGAGC TGAATCAGAG CTCGTCCGGT 
GCCGGGCTGG CGGGCGGCGT GGCGGCCTTG GTGCTGCTGG CTCTGCTGGG GACCGTGCTC 
CTTCTGCTGC ACCGCAGCGG AAGGCTCAGG TGGAGAAGGC ATGAAGACGC TGAGCCCGTA 
TCGGCTGGGC TGCCGCTGGG TTTCCGAAAT CCGATTTTCG ACGCGATTGT CTTCAAGCAG 
CAGCCTTCGG TCGAGCTACC TGACTCAGCT CAGAAGGTGG ACATCTTGGA CATTGATACC 
AAGTTCGGCT GCTTCGTTAA CCCACTCTTT GCTGGGGAGG CAGAGGCAGA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Amn ( XM_030247005.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102865.1 Mus musculus amnionless (Amn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu86175
Clone ID Related Accession (Same CDS sequence)	XM_030247005.1
Accession Version	XM_030247005.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein amnionless isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCGT GGATCTGCGC GTCTCTGCTG CAGCCCTTGG GCGGCCGCTG TCCCCAGGCC 
GCCTGCCAGG ACCCCCTACT GCCTCAAGGA CAATGCTGTG ACCTCTGCGG AGCTATCGTG 
TCGTTGACCC ACGACCCCAC ATTTGACCTG GAGCGGTACC GAGCGCGGCT GCTGGACCTC 
TTCCTGAAGC AGCCCCAGTA CCAGGGCCTG CAGGTGGCTG TGTCCAAGGT GCTGCGCGAC 
GCCCACACCG AGATCCAGGT GGTGCTGGTG GAGACCGAGC ACGCGACCGG CGCGGCGGGG 
CAGCTGGGCC ACGCGCTCCT CCAGGACGCG GTGGCACAAG GCAGCGTGCT CGGTATCGTG 
TCGGCGACCC TGCGGCAGTC CGGCAAGCCT ATGACGGCAG ACTCAGAGCT GAATCAGAGC 
TCGTCCGGTG CCGGGCTGGC GGGCGGCGTG GCGGCCTTGG TGCTGCTGGC TCTGCTGGGG 
ACCGTGCTCC TTCTGCTGCA CCGCAGCGGA AGGCTCAGGT GGAGAAGGCA TGAAGACGCT 
GAGCCCGTAT CGGCTGGGCT GCCGCTGGGT TTCCGAAATC CGATTTTCGA CGCGATTGTC 
TTCAAGCAGC AGCCTTCGGT CGAGCTACCT GACTCAGCTC AGAAGGTGGA CATCTTGGAC 
ATTGATACCA AGTTCGGCTG CTTCGTTAAC CCACTCTTTG CTGGGGAGGC AGAGGCAGAG 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102865.1
CDS	617..1342
Translation	MLPWICASLLQPLGGRCPQAACQDPLLPQGQCCDLCGAIVSLTHDPTFDLERYRARLLDLFLKQPQYQGLQVAVSKVLRDAHTEIQVVLVETEHATGAAGQLGHALLQDAVAQGSVLGIVSATLRQSGKPMTADSELNQSSSGAGLAGGVAALVLLALLGTVLLLLHRSGRLRWRRHEDAEPVSAGLPLGFRNPIFDAIVFKQQPSVELPDSAQKVDILDIDTKFGCFVNPLFAGEAEAEA

Target ORF information:

RefSeq Version	XM_030247005.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus amnionless (Amn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247005.1

ORF Insert Sequence:

ATGCTGCCGT GGATCTGCGC GTCTCTGCTG CAGCCCTTGG GCGGCCGCTG TCCCCAGGCC 
GCCTGCCAGG ACCCCCTACT GCCTCAAGGA CAATGCTGTG ACCTCTGCGG AGCTATCGTG 
TCGTTGACCC ACGACCCCAC ATTTGACCTG GAGCGGTACC GAGCGCGGCT GCTGGACCTC 
TTCCTGAAGC AGCCCCAGTA CCAGGGCCTG CAGGTGGCTG TGTCCAAGGT GCTGCGCGAC 
GCCCACACCG AGATCCAGGT GGTGCTGGTG GAGACCGAGC ACGCGACCGG CGCGGCGGGG 
CAGCTGGGCC ACGCGCTCCT CCAGGACGCG GTGGCACAAG GCAGCGTGCT CGGTATCGTG 
TCGGCGACCC TGCGGCAGTC CGGCAAGCCT ATGACGGCAG ACTCAGAGCT GAATCAGAGC 
TCGTCCGGTG CCGGGCTGGC GGGCGGCGTG GCGGCCTTGG TGCTGCTGGC TCTGCTGGGG 
ACCGTGCTCC TTCTGCTGCA CCGCAGCGGA AGGCTCAGGT GGAGAAGGCA TGAAGACGCT 
GAGCCCGTAT CGGCTGGGCT GCCGCTGGGT TTCCGAAATC CGATTTTCGA CGCGATTGTC 
TTCAAGCAGC AGCCTTCGGT CGAGCTACCT GACTCAGCTC AGAAGGTGGA CATCTTGGAC 
ATTGATACCA AGTTCGGCTG CTTCGTTAAC CCACTCTTTG CTGGGGAGGC AGAGGCAGAG 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Imprinted expression in cystic embryoid bodies shows an embryonic and not an extra-embryonic pattern.
Developmental biology402(2)291-305(2015 Jun)
Kulinski TM,Casari MR,Guenzl PM,Wenzel D,Andergassen D,Hladik A,Datlinger P,Farlik M,Theussl HC,Penninger JM,Knapp S,Bock C,Barlow DP,Hudson QJ

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Nature521(7553)520-4(2015 May)
Li Y,Klena NT,Gabriel GC,Liu X,Kim AJ,Lemke K,Chen Y,Chatterjee B,Devine W,Damerla RR,Chang C,Yagi H,San Agustin JT,Thahir M,Anderton S,Lawhead C,Vescovi A,Pratt H,Morgan J,Haynes L,Smith CL,Eppig JT,Reinholdt L,Francis R,Leatherbury L,Ganapathiraju MK,Tobita K,Pazour GJ,Lo CW

The novel helicase helG (DHX30) is expressed during gastrulation in mice and has a structure similar to a human DExH box helicase.
Stem cells and development24(3)372-83(2015 Feb)
Zheng HJ,Tsukahara M,Liu E,Ye L,Xiong H,Noguchi S,Suzuki K,Ji ZS

Expression of amnionless in mouse testes and Leydig cells.
Andrologia44 Suppl 1383-9(2012 May)
Oh YS,Park HY,Gye MC

O-fucosylation of thrombospondin type 1 repeats restricts epithelial to mesenchymal transition (EMT) and maintains epiblast pluripotency during mouse gastrulation.
Developmental biology346(1)25-38(2010 Oct)
Du J,Takeuchi H,Leonhard-Melief C,Shroyer KR,Dlugosz M,Haltiwanger RS,Holdener BC

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Amn cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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