Sirt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sirt1
Entrez Gene ID	93759
Full Name	sirtuin 1
Synonyms	AA673258,SIR2L1,Sir2,Sir2a,Sir2alpha
General protein information	Preferred Names sirtuin 1 Names NAD-dependent protein deacetylase sirtuin-1 SIR2-like protein 1 regulatory protein SIR2 homolog 1 sirtuin 1 ((silent mating type information regulation 2, homolog) 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 B4
Summary	This gene encodes a member of the sirtuin family of proteins, characterized by their deacetylase activity and proposed role in longevity. The encoded protein regulates gene expression in a wide range of cell and tissue types through its NAD+-dependent deacetylation of histones, transcription factors and transcriptional coactivators. Brain-specific overexpression of this gene has been shown to result in increased median lifespan. Viability of homozygous knockout mice for this gene varies with strain background. Homozygous knockout mice of strains that do not exhibit embryonic lethality are sterile and have a reduced lifespan. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019812.2	NP_062786.1	NAD-dependent protein deacetylase sirtuin-1 isoform 1
XM_011243606.1	XP_011241908.1	NAD-dependent protein deacetylase sirtuin-1 isoform X1
NM_019812.3	NP_062786.1	NAD-dependent protein deacetylase sirtuin-1 isoform 1
NM_001159589.2	NP_001153061.1	NAD-dependent protein deacetylase sirtuin-1 isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SIRT1	XP_003312628.1
Rattus norvegicus (Norway rat)	Sirt1	XP_006256208.1
Xenopus tropicalis (tropical clawed frog)	sirt1	NP_001136381.1
Homo sapiens (human)	SIRT1	NP_036370.2
Canis lupus familiaris (dog)	SIRT1	XP_546130.2
Gallus gallus (chicken)	SIRT1	NP_001004767.1
	SIRT1	XP_001087854.2
Danio rerio (zebrafish)	sirt1	XP_005173091.1
Bos taurus (cattle)	SIRT1	NP_001179909.1
Mus musculus (house mouse)	Sirt1	NP_062786.1

Gene Ontology

The following Sirt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sirt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu71898	XM_011243606.1 Latest version!	Mus musculus sirtuin 1 (Sirt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu22520	NM_019812.3 Latest version!	Mus musculus sirtuin 1 (Sirt1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu22481	NM_001159589.2 Latest version!	Mus musculus sirtuin 1 (Sirt1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OMu22520	NM_019812.2	Mus musculus sirtuin 1 (Sirt1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sirt1 ( XM_011243606.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241908.1 Mus musculus sirtuin 1 (Sirt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu71898
Clone ID Related Accession (Same CDS sequence)	XM_011243606.1
Accession Version	XM_011243606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	NAD-dependent protein deacetylase sirtuin-1 isoform X1
Comment

ATGTTTGATA TTGAGTATTT TAGAAAAGAC CCAAGACCAT TCTTCAAGTT TGCAAAGGAA 
ATATATCCCG GACAGTTCCA GCCGTCTCTG TGTCACAAAT TCATAGCTTT GTCAGATAAG 
GAAGGAAAAC TACTTCGAAA TTATACTCAA AATATAGATA CCTTGGAGCA GGTTGCAGGA 
ATCCAAAGGA TCCTTCAGTG TCATGGTTCC TTTGCAACAG CATCTTGCCT GATTTGTAAA 
TACAAAGTTG ATTGTGAAGC TGTTCGTGGA GACATTTTTA ATCAGGTAGT TCCTCGGTGC 
CCTAGGTGCC CAGCTGATGA GCCACTTGCC ATCATGAAGC CAGAGATTGT CTTCTTTGGT 
GAAAACTTAC CAGAACAGTT TCATAGAGCC ATGAAGTATG ACAAAGATGA AGTTGACCTC 
CTCATTGTTA TTGGATCTTC TCTGAAAGTG AGACCAGTAG CACTAATTCC AAGTTCTATA 
CCCCATGAAG TGCCTCAAAT ATTAATAAAT AGGGAACCTT TGCCTCATCT ACATTTTGAT 
GTAGAGCTCC TTGGAGACTG CGATGTTATA ATTAATGAGT TGTGTCATAG GCTAGGTGGT 
GAATATGCCA AACTTTGTTG TAACCCTGTA AAGCTTTCAG AAATTACTGA AAAACCTCCA 
CGCCCACAAA AGGAATTGGT TCATTTATCA GAGTTGCCAC CAACACCTCT TCATATTTCG 
GAAGACTCAA GTTCACCTGA AAGAACTGTA CCACAAGACT CTTCTGTGAT TGCTACACTT 
GTAGACCAAG CAACAAACAA CAATGTTAAT GATTTAGAAG TATCTGAATC AAGTTGTGTG 
GAAGAAAAAC CACAAGAAGT ACAGACTAGT AGGAATGTTG AGAACATTAA TGTGGAAAAT 
CCAGATTTTA AGGCTGTTGG TTCCAGTACT GCAGACAAAA ATGAAAGAAC TTCAGTTGCA 
GAAACAGTGA GAAAATGCTG GCCTAATAGA CTTGCAAAGG AGCAGATTAG TAAGCGGCTT 
GAGGGTAATC AATACCTGTT TGTACCACCA AATCGTTACA TATTCCACGG TGCTGAGGTA 
TACTCAGACT CTGAAGATGA CGTCTTGTCC TCTAGTTCCT GTGGCAGTAA CAGTGACAGT 
GGCACATGCC AGAGTCCAAG TTTAGAAGAA CCCTTGGAAG ATGAAAGTGA AATTGAAGAA 
TTCTACAATG GCTTGGAAGA TGATACGGAG AGGCCCGAAT GTGCTGGAGG ATCTGGATTT 
GGAGCTGATG GAGGGGATCA AGAGGTTGTT AATGAAGCTA TAGCTACAAG ACAGGAATTG 
ACAGATGTAA ACTATCCATC AGACAAATCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011241908.1

Translation

MFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRILQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRPQKELVHLSELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLEVSESSCVEEKPQEVQTSRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGNQYLFVPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLEDDTERPECAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS

Target ORF information:

RefSeq Version	XM_011243606.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus sirtuin 1 (Sirt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243606.1

ORF Insert Sequence:

ATGTTTGATA TTGAGTATTT TAGAAAAGAC CCAAGACCAT TCTTCAAGTT TGCAAAGGAA 
ATATATCCCG GACAGTTCCA GCCGTCTCTG TGTCACAAAT TCATAGCTTT GTCAGATAAG 
GAAGGAAAAC TACTTCGAAA TTATACTCAA AATATAGATA CCTTGGAGCA GGTTGCAGGA 
ATCCAAAGGA TCCTTCAGTG TCATGGTTCC TTTGCAACAG CATCTTGCCT GATTTGTAAA 
TACAAAGTTG ATTGTGAAGC TGTTCGTGGA GACATTTTTA ATCAGGTAGT TCCTCGGTGC 
CCTAGGTGCC CAGCTGATGA GCCACTTGCC ATCATGAAGC CAGAGATTGT CTTCTTTGGT 
GAAAACTTAC CAGAACAGTT TCATAGAGCC ATGAAGTATG ACAAAGATGA AGTTGACCTC 
CTCATTGTTA TTGGATCTTC TCTGAAAGTG AGACCAGTAG CACTAATTCC AAGTTCTATA 
CCCCATGAAG TGCCTCAAAT ATTAATAAAT AGGGAACCTT TGCCTCATCT ACATTTTGAT 
GTAGAGCTCC TTGGAGACTG CGATGTTATA ATTAATGAGT TGTGTCATAG GCTAGGTGGT 
GAATATGCCA AACTTTGTTG TAACCCTGTA AAGCTTTCAG AAATTACTGA AAAACCTCCA 
CGCCCACAAA AGGAATTGGT TCATTTATCA GAGTTGCCAC CAACACCTCT TCATATTTCG 
GAAGACTCAA GTTCACCTGA AAGAACTGTA CCACAAGACT CTTCTGTGAT TGCTACACTT 
GTAGACCAAG CAACAAACAA CAATGTTAAT GATTTAGAAG TATCTGAATC AAGTTGTGTG 
GAAGAAAAAC CACAAGAAGT ACAGACTAGT AGGAATGTTG AGAACATTAA TGTGGAAAAT 
CCAGATTTTA AGGCTGTTGG TTCCAGTACT GCAGACAAAA ATGAAAGAAC TTCAGTTGCA 
GAAACAGTGA GAAAATGCTG GCCTAATAGA CTTGCAAAGG AGCAGATTAG TAAGCGGCTT 
GAGGGTAATC AATACCTGTT TGTACCACCA AATCGTTACA TATTCCACGG TGCTGAGGTA 
TACTCAGACT CTGAAGATGA CGTCTTGTCC TCTAGTTCCT GTGGCAGTAA CAGTGACAGT 
GGCACATGCC AGAGTCCAAG TTTAGAAGAA CCCTTGGAAG ATGAAAGTGA AATTGAAGAA 
TTCTACAATG GCTTGGAAGA TGATACGGAG AGGCCCGAAT GTGCTGGAGG ATCTGGATTT 
GGAGCTGATG GAGGGGATCA AGAGGTTGTT AATGAAGCTA TAGCTACAAG ACAGGAATTG 
ACAGATGTAA ACTATCCATC AGACAAATCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sirt1 ( NM_019812.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062786.1 Mus musculus sirtuin 1 (Sirt1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22520
Clone ID Related Accession (Same CDS sequence)	NM_019812.2 , NM_019812.3
Accession Version	NM_019812.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	NAD-dependent protein deacetylase sirtuin-1 isoform 1
Comment

ATGGCGGACG AGGTGGCGCT CGCCCTTCAG GCCGCCGGCT CCCCTTCCGC GGCGGCCGCC 
ATGGAGGCCG CGTCGCAGCC GGCGGACGAG CCGCTCCGCA AGAGGCCCCG CCGAGACGGG 
CCTGGCCTCG GGCGCAGCCC GGGCGAGCCG AGCGCAGCAG TGGCGCCGGC GGCCGCGGGG 
TGTGAGGCGG CGAGCGCCGC GGCCCCGGCG GCGCTGTGGC GGGAGGCGGC AGGGGCGGCG 
GCGAGCGCGG AGCGGGAGGC CCCGGCGACG GCCGTGGCCG GGGACGGAGA CAATGGGTCC 
GGCCTGCGGC GGGAGCCGAG GGCGGCTGAC GACTTCGACG ACGACGAGGG CGAGGAGGAG 
GACGAGGCGG CGGCGGCAGC GGCGGCGGCA GCGATCGGCT ACCGAGACAA CCTCCTGTTG 
ACCGATGGAC TCCTCACTAA TGGCTTTCAT TCCTGTGAAA GTGATGACGA TGACAGAACG 
TCACACGCCA GCTCTAGTGA CTGGACTCCG CGGCCGCGGA TAGGTCCATA TACTTTTGTT 
CAGCAACATC TCATGATTGG CACCGATCCT CGAACAATTC TTAAAGATTT ATTACCAGAA 
ACAATTCCTC CACCTGAGCT GGATGATATG ACGCTGTGGC AGATTGTTAT TAATATCCTT 
TCAGAACCAC CAAAGCGGAA AAAAAGAAAA GATATCAATA CAATTGAAGA TGCTGTGAAG 
TTACTGCAGG AGTGTAAAAA GATAATAGTT CTGACTGGAG CTGGGGTTTC TGTCTCCTGT 
GGGATTCCTG ACTTCAGATC AAGAGACGGT ATCTATGCTC GCCTTGCGGT GGACTTCCCA 
GACCTCCCAG ACCCTCAAGC CATGTTTGAT ATTGAGTATT TTAGAAAAGA CCCAAGACCA 
TTCTTCAAGT TTGCAAAGGA AATATATCCC GGACAGTTCC AGCCGTCTCT GTGTCACAAA 
TTCATAGCTT TGTCAGATAA GGAAGGAAAA CTACTTCGAA ATTATACTCA AAATATAGAT 
ACCTTGGAGC AGGTTGCAGG AATCCAAAGG ATCCTTCAGT GTCATGGTTC CTTTGCAACA 
GCATCTTGCC TGATTTGTAA ATACAAAGTT GATTGTGAAG CTGTTCGTGG AGACATTTTT 
AATCAGGTAG TTCCTCGGTG CCCTAGGTGC CCAGCTGATG AGCCACTTGC CATCATGAAG 
CCAGAGATTG TCTTCTTTGG TGAAAACTTA CCAGAACAGT TTCATAGAGC CATGAAGTAT 
GACAAAGATG AAGTTGACCT CCTCATTGTT ATTGGATCTT CTCTGAAAGT GAGACCAGTA 
GCACTAATTC CAAGTTCTAT ACCCCATGAA GTGCCTCAAA TATTAATAAA TAGGGAACCT 
TTGCCTCATC TACATTTTGA TGTAGAGCTC CTTGGAGACT GCGATGTTAT AATTAATGAG 
TTGTGTCATA GGCTAGGTGG TGAATATGCC AAACTTTGTT GTAACCCTGT AAAGCTTTCA 
GAAATTACTG AAAAACCTCC ACGCCCACAA AAGGAATTGG TTCATTTATC AGAGTTGCCA 
CCAACACCTC TTCATATTTC GGAAGACTCA AGTTCACCTG AAAGAACTGT ACCACAAGAC 
TCTTCTGTGA TTGCTACACT TGTAGACCAA GCAACAAACA ACAATGTTAA TGATTTAGAA 
GTATCTGAAT CAAGTTGTGT GGAAGAAAAA CCACAAGAAG TACAGACTAG TAGGAATGTT 
GAGAACATTA ATGTGGAAAA TCCAGATTTT AAGGCTGTTG GTTCCAGTAC TGCAGACAAA 
AATGAAAGAA CTTCAGTTGC AGAAACAGTG AGAAAATGCT GGCCTAATAG ACTTGCAAAG 
GAGCAGATTA GTAAGCGGCT TGAGGGTAAT CAATACCTGT TTGTACCACC AAATCGTTAC 
ATATTCCACG GTGCTGAGGT ATACTCAGAC TCTGAAGATG ACGTCTTGTC CTCTAGTTCC 
TGTGGCAGTA ACAGTGACAG TGGCACATGC CAGAGTCCAA GTTTAGAAGA ACCCTTGGAA 
GATGAAAGTG AAATTGAAGA ATTCTACAAT GGCTTGGAAG ATGATACGGA GAGGCCCGAA 
TGTGCTGGAG GATCTGGATT TGGAGCTGAT GGAGGGGATC AAGAGGTTGT TAATGAAGCT 
ATAGCTACAA GACAGGAATT GACAGATGTA AACTATCCAT CAGACAAATC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_062786.1

Translation

MADEVALALQAAGSPSAAAAMEAASQPADEPLRKRPRRDGPGLGRSPGEPSAAVAPAAAGCEAASAAAPAALWREAAGAAASAEREAPATAVAGDGDNGSGLRREPRAADDFDDDEGEEEDEAAAAAAAAAIGYRDNLLLTDGLLTNGFHSCESDDDDRTSHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRILQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRPQKELVHLSELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLEVSESSCVEEKPQEVQTSRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGNQYLFVPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLEDDTERPECAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS

Target ORF information:

RefSeq Version	NM_019812.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus sirtuin 1 (Sirt1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019812.2

ORF Insert Sequence:

ATGGCGGACG AGGTGGCGCT CGCCCTTCAG GCCGCCGGCT CCCCTTCCGC GGCGGCCGCC 
ATGGAGGCCG CGTCGCAGCC GGCGGACGAG CCGCTCCGCA AGAGGCCCCG CCGAGACGGG 
CCTGGCCTCG GGCGCAGCCC GGGCGAGCCG AGCGCAGCAG TGGCGCCGGC GGCCGCGGGG 
TGTGAGGCGG CGAGCGCCGC GGCCCCGGCG GCGCTGTGGC GGGAGGCGGC AGGGGCGGCG 
GCGAGCGCGG AGCGGGAGGC CCCGGCGACG GCCGTGGCCG GGGACGGAGA CAATGGGTCC 
GGCCTGCGGC GGGAGCCGAG GGCGGCTGAC GACTTCGACG ACGACGAGGG CGAGGAGGAG 
GACGAGGCGG CGGCGGCAGC GGCGGCGGCA GCGATCGGCT ACCGAGACAA CCTCCTGTTG 
ACCGATGGAC TCCTCACTAA TGGCTTTCAT TCCTGTGAAA GTGATGACGA TGACAGAACG 
TCACACGCCA GCTCTAGTGA CTGGACTCCG CGGCCGCGGA TAGGTCCATA TACTTTTGTT 
CAGCAACATC TCATGATTGG CACCGATCCT CGAACAATTC TTAAAGATTT ATTACCAGAA 
ACAATTCCTC CACCTGAGCT GGATGATATG ACGCTGTGGC AGATTGTTAT TAATATCCTT 
TCAGAACCAC CAAAGCGGAA AAAAAGAAAA GATATCAATA CAATTGAAGA TGCTGTGAAG 
TTACTGCAGG AGTGTAAAAA GATAATAGTT CTGACTGGAG CTGGGGTTTC TGTCTCCTGT 
GGGATTCCTG ACTTCAGATC AAGAGACGGT ATCTATGCTC GCCTTGCGGT GGACTTCCCA 
GACCTCCCAG ACCCTCAAGC CATGTTTGAT ATTGAGTATT TTAGAAAAGA CCCAAGACCA 
TTCTTCAAGT TTGCAAAGGA AATATATCCC GGACAGTTCC AGCCGTCTCT GTGTCACAAA 
TTCATAGCTT TGTCAGATAA GGAAGGAAAA CTACTTCGAA ATTATACTCA AAATATAGAT 
ACCTTGGAGC AGGTTGCAGG AATCCAAAGG ATCCTTCAGT GTCATGGTTC CTTTGCAACA 
GCATCTTGCC TGATTTGTAA ATACAAAGTT GATTGTGAAG CTGTTCGTGG AGACATTTTT 
AATCAGGTAG TTCCTCGGTG CCCTAGGTGC CCAGCTGATG AGCCACTTGC CATCATGAAG 
CCAGAGATTG TCTTCTTTGG TGAAAACTTA CCAGAACAGT TTCATAGAGC CATGAAGTAT 
GACAAAGATG AAGTTGACCT CCTCATTGTT ATTGGATCTT CTCTGAAAGT GAGACCAGTA 
GCACTAATTC CAAGTTCTAT ACCCCATGAA GTGCCTCAAA TATTAATAAA TAGGGAACCT 
TTGCCTCATC TACATTTTGA TGTAGAGCTC CTTGGAGACT GCGATGTTAT AATTAATGAG 
TTGTGTCATA GGCTAGGTGG TGAATATGCC AAACTTTGTT GTAACCCTGT AAAGCTTTCA 
GAAATTACTG AAAAACCTCC ACGCCCACAA AAGGAATTGG TTCATTTATC AGAGTTGCCA 
CCAACACCTC TTCATATTTC GGAAGACTCA AGTTCACCTG AAAGAACTGT ACCACAAGAC 
TCTTCTGTGA TTGCTACACT TGTAGACCAA GCAACAAACA ACAATGTTAA TGATTTAGAA 
GTATCTGAAT CAAGTTGTGT GGAAGAAAAA CCACAAGAAG TACAGACTAG TAGGAATGTT 
GAGAACATTA ATGTGGAAAA TCCAGATTTT AAGGCTGTTG GTTCCAGTAC TGCAGACAAA 
AATGAAAGAA CTTCAGTTGC AGAAACAGTG AGAAAATGCT GGCCTAATAG ACTTGCAAAG 
GAGCAGATTA GTAAGCGGCT TGAGGGTAAT CAATACCTGT TTGTACCACC AAATCGTTAC 
ATATTCCACG GTGCTGAGGT ATACTCAGAC TCTGAAGATG ACGTCTTGTC CTCTAGTTCC 
TGTGGCAGTA ACAGTGACAG TGGCACATGC CAGAGTCCAA GTTTAGAAGA ACCCTTGGAA 
GATGAAAGTG AAATTGAAGA ATTCTACAAT GGCTTGGAAG ATGATACGGA GAGGCCCGAA 
TGTGCTGGAG GATCTGGATT TGGAGCTGAT GGAGGGGATC AAGAGGTTGT TAATGAAGCT 
ATAGCTACAA GACAGGAATT GACAGATGTA AACTATCCAT CAGACAAATC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sirt1 ( NM_019812.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062786.1 Mus musculus sirtuin 1 (Sirt1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22520
Clone ID Related Accession (Same CDS sequence)	NM_019812.2 , NM_019812.3
Accession Version	NM_019812.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	NAD-dependent protein deacetylase sirtuin-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC153516.8, AK140330.1, BE989110.1, BX523319.1, AK139946.1 and AK141245.1. On Sep 29, 2015 this sequence version replaced NM_019812.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG AGGTGGCGCT CGCCCTTCAG GCCGCCGGCT CCCCTTCCGC GGCGGCCGCC 
ATGGAGGCCG CGTCGCAGCC GGCGGACGAG CCGCTCCGCA AGAGGCCCCG CCGAGACGGG 
CCTGGCCTCG GGCGCAGCCC GGGCGAGCCG AGCGCAGCAG TGGCGCCGGC GGCCGCGGGG 
TGTGAGGCGG CGAGCGCCGC GGCCCCGGCG GCGCTGTGGC GGGAGGCGGC AGGGGCGGCG 
GCGAGCGCGG AGCGGGAGGC CCCGGCGACG GCCGTGGCCG GGGACGGAGA CAATGGGTCC 
GGCCTGCGGC GGGAGCCGAG GGCGGCTGAC GACTTCGACG ACGACGAGGG CGAGGAGGAG 
GACGAGGCGG CGGCGGCAGC GGCGGCGGCA GCGATCGGCT ACCGAGACAA CCTCCTGTTG 
ACCGATGGAC TCCTCACTAA TGGCTTTCAT TCCTGTGAAA GTGATGACGA TGACAGAACG 
TCACACGCCA GCTCTAGTGA CTGGACTCCG CGGCCGCGGA TAGGTCCATA TACTTTTGTT 
CAGCAACATC TCATGATTGG CACCGATCCT CGAACAATTC TTAAAGATTT ATTACCAGAA 
ACAATTCCTC CACCTGAGCT GGATGATATG ACGCTGTGGC AGATTGTTAT TAATATCCTT 
TCAGAACCAC CAAAGCGGAA AAAAAGAAAA GATATCAATA CAATTGAAGA TGCTGTGAAG 
TTACTGCAGG AGTGTAAAAA GATAATAGTT CTGACTGGAG CTGGGGTTTC TGTCTCCTGT 
GGGATTCCTG ACTTCAGATC AAGAGACGGT ATCTATGCTC GCCTTGCGGT GGACTTCCCA 
GACCTCCCAG ACCCTCAAGC CATGTTTGAT ATTGAGTATT TTAGAAAAGA CCCAAGACCA 
TTCTTCAAGT TTGCAAAGGA AATATATCCC GGACAGTTCC AGCCGTCTCT GTGTCACAAA 
TTCATAGCTT TGTCAGATAA GGAAGGAAAA CTACTTCGAA ATTATACTCA AAATATAGAT 
ACCTTGGAGC AGGTTGCAGG AATCCAAAGG ATCCTTCAGT GTCATGGTTC CTTTGCAACA 
GCATCTTGCC TGATTTGTAA ATACAAAGTT GATTGTGAAG CTGTTCGTGG AGACATTTTT 
AATCAGGTAG TTCCTCGGTG CCCTAGGTGC CCAGCTGATG AGCCACTTGC CATCATGAAG 
CCAGAGATTG TCTTCTTTGG TGAAAACTTA CCAGAACAGT TTCATAGAGC CATGAAGTAT 
GACAAAGATG AAGTTGACCT CCTCATTGTT ATTGGATCTT CTCTGAAAGT GAGACCAGTA 
GCACTAATTC CAAGTTCTAT ACCCCATGAA GTGCCTCAAA TATTAATAAA TAGGGAACCT 
TTGCCTCATC TACATTTTGA TGTAGAGCTC CTTGGAGACT GCGATGTTAT AATTAATGAG 
TTGTGTCATA GGCTAGGTGG TGAATATGCC AAACTTTGTT GTAACCCTGT AAAGCTTTCA 
GAAATTACTG AAAAACCTCC ACGCCCACAA AAGGAATTGG TTCATTTATC AGAGTTGCCA 
CCAACACCTC TTCATATTTC GGAAGACTCA AGTTCACCTG AAAGAACTGT ACCACAAGAC 
TCTTCTGTGA TTGCTACACT TGTAGACCAA GCAACAAACA ACAATGTTAA TGATTTAGAA 
GTATCTGAAT CAAGTTGTGT GGAAGAAAAA CCACAAGAAG TACAGACTAG TAGGAATGTT 
GAGAACATTA ATGTGGAAAA TCCAGATTTT AAGGCTGTTG GTTCCAGTAC TGCAGACAAA 
AATGAAAGAA CTTCAGTTGC AGAAACAGTG AGAAAATGCT GGCCTAATAG ACTTGCAAAG 
GAGCAGATTA GTAAGCGGCT TGAGGGTAAT CAATACCTGT TTGTACCACC AAATCGTTAC 
ATATTCCACG GTGCTGAGGT ATACTCAGAC TCTGAAGATG ACGTCTTGTC CTCTAGTTCC 
TGTGGCAGTA ACAGTGACAG TGGCACATGC CAGAGTCCAA GTTTAGAAGA ACCCTTGGAA 
GATGAAAGTG AAATTGAAGA ATTCTACAAT GGCTTGGAAG ATGATACGGA GAGGCCCGAA 
TGTGCTGGAG GATCTGGATT TGGAGCTGAT GGAGGGGATC AAGAGGTTGT TAATGAAGCT 
ATAGCTACAA GACAGGAATT GACAGATGTA AACTATCCAT CAGACAAATC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062786.1
CDS	66..2279
Translation	MADEVALALQAAGSPSAAAAMEAASQPADEPLRKRPRRDGPGLGRSPGEPSAAVAPAAAGCEAASAAAPAALWREAAGAAASAEREAPATAVAGDGDNGSGLRREPRAADDFDDDEGEEEDEAAAAAAAAAIGYRDNLLLTDGLLTNGFHSCESDDDDRTSHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRILQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRPQKELVHLSELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLEVSESSCVEEKPQEVQTSRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGNQYLFVPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLEDDTERPECAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS

Target ORF information:

RefSeq Version	NM_019812.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sirtuin 1 (Sirt1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019812.3

ORF Insert Sequence:

ATGGCGGACG AGGTGGCGCT CGCCCTTCAG GCCGCCGGCT CCCCTTCCGC GGCGGCCGCC 
ATGGAGGCCG CGTCGCAGCC GGCGGACGAG CCGCTCCGCA AGAGGCCCCG CCGAGACGGG 
CCTGGCCTCG GGCGCAGCCC GGGCGAGCCG AGCGCAGCAG TGGCGCCGGC GGCCGCGGGG 
TGTGAGGCGG CGAGCGCCGC GGCCCCGGCG GCGCTGTGGC GGGAGGCGGC AGGGGCGGCG 
GCGAGCGCGG AGCGGGAGGC CCCGGCGACG GCCGTGGCCG GGGACGGAGA CAATGGGTCC 
GGCCTGCGGC GGGAGCCGAG GGCGGCTGAC GACTTCGACG ACGACGAGGG CGAGGAGGAG 
GACGAGGCGG CGGCGGCAGC GGCGGCGGCA GCGATCGGCT ACCGAGACAA CCTCCTGTTG 
ACCGATGGAC TCCTCACTAA TGGCTTTCAT TCCTGTGAAA GTGATGACGA TGACAGAACG 
TCACACGCCA GCTCTAGTGA CTGGACTCCG CGGCCGCGGA TAGGTCCATA TACTTTTGTT 
CAGCAACATC TCATGATTGG CACCGATCCT CGAACAATTC TTAAAGATTT ATTACCAGAA 
ACAATTCCTC CACCTGAGCT GGATGATATG ACGCTGTGGC AGATTGTTAT TAATATCCTT 
TCAGAACCAC CAAAGCGGAA AAAAAGAAAA GATATCAATA CAATTGAAGA TGCTGTGAAG 
TTACTGCAGG AGTGTAAAAA GATAATAGTT CTGACTGGAG CTGGGGTTTC TGTCTCCTGT 
GGGATTCCTG ACTTCAGATC AAGAGACGGT ATCTATGCTC GCCTTGCGGT GGACTTCCCA 
GACCTCCCAG ACCCTCAAGC CATGTTTGAT ATTGAGTATT TTAGAAAAGA CCCAAGACCA 
TTCTTCAAGT TTGCAAAGGA AATATATCCC GGACAGTTCC AGCCGTCTCT GTGTCACAAA 
TTCATAGCTT TGTCAGATAA GGAAGGAAAA CTACTTCGAA ATTATACTCA AAATATAGAT 
ACCTTGGAGC AGGTTGCAGG AATCCAAAGG ATCCTTCAGT GTCATGGTTC CTTTGCAACA 
GCATCTTGCC TGATTTGTAA ATACAAAGTT GATTGTGAAG CTGTTCGTGG AGACATTTTT 
AATCAGGTAG TTCCTCGGTG CCCTAGGTGC CCAGCTGATG AGCCACTTGC CATCATGAAG 
CCAGAGATTG TCTTCTTTGG TGAAAACTTA CCAGAACAGT TTCATAGAGC CATGAAGTAT 
GACAAAGATG AAGTTGACCT CCTCATTGTT ATTGGATCTT CTCTGAAAGT GAGACCAGTA 
GCACTAATTC CAAGTTCTAT ACCCCATGAA GTGCCTCAAA TATTAATAAA TAGGGAACCT 
TTGCCTCATC TACATTTTGA TGTAGAGCTC CTTGGAGACT GCGATGTTAT AATTAATGAG 
TTGTGTCATA GGCTAGGTGG TGAATATGCC AAACTTTGTT GTAACCCTGT AAAGCTTTCA 
GAAATTACTG AAAAACCTCC ACGCCCACAA AAGGAATTGG TTCATTTATC AGAGTTGCCA 
CCAACACCTC TTCATATTTC GGAAGACTCA AGTTCACCTG AAAGAACTGT ACCACAAGAC 
TCTTCTGTGA TTGCTACACT TGTAGACCAA GCAACAAACA ACAATGTTAA TGATTTAGAA 
GTATCTGAAT CAAGTTGTGT GGAAGAAAAA CCACAAGAAG TACAGACTAG TAGGAATGTT 
GAGAACATTA ATGTGGAAAA TCCAGATTTT AAGGCTGTTG GTTCCAGTAC TGCAGACAAA 
AATGAAAGAA CTTCAGTTGC AGAAACAGTG AGAAAATGCT GGCCTAATAG ACTTGCAAAG 
GAGCAGATTA GTAAGCGGCT TGAGGGTAAT CAATACCTGT TTGTACCACC AAATCGTTAC 
ATATTCCACG GTGCTGAGGT ATACTCAGAC TCTGAAGATG ACGTCTTGTC CTCTAGTTCC 
TGTGGCAGTA ACAGTGACAG TGGCACATGC CAGAGTCCAA GTTTAGAAGA ACCCTTGGAA 
GATGAAAGTG AAATTGAAGA ATTCTACAAT GGCTTGGAAG ATGATACGGA GAGGCCCGAA 
TGTGCTGGAG GATCTGGATT TGGAGCTGAT GGAGGGGATC AAGAGGTTGT TAATGAAGCT 
ATAGCTACAA GACAGGAATT GACAGATGTA AACTATCCAT CAGACAAATC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sirt1 ( NM_001159589.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153061.1 Mus musculus sirtuin 1 (Sirt1), transcript variant 2, mRNA.

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CloneID	OMu22481
Clone ID Related Accession (Same CDS sequence)	NM_001159589.2
Accession Version	NM_001159589.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	NAD-dependent protein deacetylase sirtuin-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC153516.8, AK140330.1, BE989110.1, BX523319.1, AK139946.1 and AK141245.1. On Sep 29, 2015 this sequence version replaced NM_001159589.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK144203.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG AGGTGGCGCT CGCCCTTCAG GCCGCCGGCT CCCCTTCCGC GGCGGCCGCC 
ATGGAGGCCG CGTCGCAGCC GGCGGACGAG CCGCTCCGCA AGAGGCCCCG CCGAGACGGG 
CCTGGCCTCG GGCGCAGCCC GGGCGAGCCG AGCGCAGCAG TGGCGCCGGC GGCCGCGGGG 
TGTGAGGCGG CGAGCGCCGC GGCCCCGGCG GCGCTGTGGC GGGAGGCGGC AGGGGCGGCG 
GCGAGCGCGG AGCGGGAGGC CCCGGCGACG GCCGTGGCCG GGGACGGAGA CAATGGGTCC 
GGCCTGCGGC GGGAGCCGAG GGCGGCTGAC GACTTCGACG ACGACGAGGG CGAGGAGGAG 
GACGAGGCGG CGGCGGCAGC GGCGGCGGCA GCGATCGGCT ACCGAGGTCC ATATACTTTT 
GTTCAGCAAC ATCTCATGAT TGGCACCGAT CCTCGAACAA TTCTTAAAGA TTTATTACCA 
GAAACAATTC CTCCACCTGA GCTGGATGAT ATGACGCTGT GGCAGATTGT TATTAATATC 
CTTTCAGAAC CACCAAAGCG GAAAAAAAGA AAAGATATCA ATACAATTGA AGATGCTGTG 
AAGTTACTGC AGGAGTGTAA AAAGATAATA GTTCTGACTG GAGCTGGGGT TTCTGTCTCC 
TGTGGGATTC CTGACTTCAG ATCAAGAGAC GGTATCTATG CTCGCCTTGC GGTGGACTTC 
CCAGACCTCC CAGACCCTCA AGCCATGTTT GATATTGAGT ATTTTAGAAA AGACCCAAGA 
CCATTCTTCA AGTTTGCAAA GGAAATATAT CCCGGACAGT TCCAGCCGTC TCTGTGTCAC 
AAATTCATAG CTTTGTCAGA TAAGGAAGGA AAACTACTTC GAAATTATAC TCAAAATATA 
GATACCTTGG AGCAGGTTGC AGGAATCCAA AGGATCCTTC AGTGTCATGG TTCCTTTGCA 
ACAGCATCTT GCCTGATTTG TAAATACAAA GTTGATTGTG AAGCTGTTCG TGGAGACATT 
TTTAATCAGG TAGTTCCTCG GTGCCCTAGG TGCCCAGCTG ATGAGCCACT TGCCATCATG 
AAGCCAGAGA TTGTCTTCTT TGGTGAAAAC TTACCAGAAC AGTTTCATAG AGCCATGAAG 
TATGACAAAG ATGAAGTTGA CCTCCTCATT GTTATTGGAT CTTCTCTGAA AGTGAGACCA 
GTAGCACTAA TTCCAAGTTC TATACCCCAT GAAGTGCCTC AAATATTAAT AAATAGGGAA 
CCTTTGCCTC ATCTACATTT TGATGTAGAG CTCCTTGGAG ACTGCGATGT TATAATTAAT 
GAGTTGTGTC ATAGGCTAGG TGGTGAATAT GCCAAACTTT GTTGTAACCC TGTAAAGCTT 
TCAGAAATTA CTGAAAAACC TCCACGCCCA CAAAAGGAAT TGGTTCATTT ATCAGAGTTG 
CCACCAACAC CTCTTCATAT TTCGGAAGAC TCAAGTTCAC CTGAAAGAAC TGTACCACAA 
GACTCTTCTG TGATTGCTAC ACTTGTAGAC CAAGCAACAA ACAACAATGT TAATGATTTA 
GAAGTATCTG AATCAAGTTG TGTGGAAGAA AAACCACAAG AAGTACAGAC TAGTAGGAAT 
GTTGAGAACA TTAATGTGGA AAATCCAGAT TTTAAGGCTG TTGGTTCCAG TACTGCAGAC 
AAAAATGAAA GAACTTCAGT TGCAGAAACA GTGAGAAAAT GCTGGCCTAA TAGACTTGCA 
AAGGAGCAGA TTAGTAAGCG GCTTGAGGGT AATCAATACC TGTTTGTACC ACCAAATCGT 
TACATATTCC ACGGTGCTGA GGTATACTCA GACTCTGAAG ATGACGTCTT GTCCTCTAGT 
TCCTGTGGCA GTAACAGTGA CAGTGGCACA TGCCAGAGTC CAAGTTTAGA AGAACCCTTG 
GAAGATGAAA GTGAAATTGA AGAATTCTAC AATGGCTTGG AAGATGATAC GGAGAGGCCC 
GAATGTGCTG GAGGATCTGG ATTTGGAGCT GATGGAGGGG ATCAAGAGGT TGTTAATGAA 
GCTATAGCTA CAAGACAGGA ATTGACAGAT GTAAACTATC CATCAGACAA ATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153061.1
CDS	66..2162
Translation	MADEVALALQAAGSPSAAAAMEAASQPADEPLRKRPRRDGPGLGRSPGEPSAAVAPAAAGCEAASAAAPAALWREAAGAAASAEREAPATAVAGDGDNGSGLRREPRAADDFDDDEGEEEDEAAAAAAAAAIGYRGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRILQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRPQKELVHLSELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLEVSESSCVEEKPQEVQTSRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGNQYLFVPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLEDDTERPECAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS

Target ORF information:

RefSeq Version	NM_001159589.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sirtuin 1 (Sirt1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159589.2

ORF Insert Sequence:

ATGGCGGACG AGGTGGCGCT CGCCCTTCAG GCCGCCGGCT CCCCTTCCGC GGCGGCCGCC 
ATGGAGGCCG CGTCGCAGCC GGCGGACGAG CCGCTCCGCA AGAGGCCCCG CCGAGACGGG 
CCTGGCCTCG GGCGCAGCCC GGGCGAGCCG AGCGCAGCAG TGGCGCCGGC GGCCGCGGGG 
TGTGAGGCGG CGAGCGCCGC GGCCCCGGCG GCGCTGTGGC GGGAGGCGGC AGGGGCGGCG 
GCGAGCGCGG AGCGGGAGGC CCCGGCGACG GCCGTGGCCG GGGACGGAGA CAATGGGTCC 
GGCCTGCGGC GGGAGCCGAG GGCGGCTGAC GACTTCGACG ACGACGAGGG CGAGGAGGAG 
GACGAGGCGG CGGCGGCAGC GGCGGCGGCA GCGATCGGCT ACCGAGGTCC ATATACTTTT 
GTTCAGCAAC ATCTCATGAT TGGCACCGAT CCTCGAACAA TTCTTAAAGA TTTATTACCA 
GAAACAATTC CTCCACCTGA GCTGGATGAT ATGACGCTGT GGCAGATTGT TATTAATATC 
CTTTCAGAAC CACCAAAGCG GAAAAAAAGA AAAGATATCA ATACAATTGA AGATGCTGTG 
AAGTTACTGC AGGAGTGTAA AAAGATAATA GTTCTGACTG GAGCTGGGGT TTCTGTCTCC 
TGTGGGATTC CTGACTTCAG ATCAAGAGAC GGTATCTATG CTCGCCTTGC GGTGGACTTC 
CCAGACCTCC CAGACCCTCA AGCCATGTTT GATATTGAGT ATTTTAGAAA AGACCCAAGA 
CCATTCTTCA AGTTTGCAAA GGAAATATAT CCCGGACAGT TCCAGCCGTC TCTGTGTCAC 
AAATTCATAG CTTTGTCAGA TAAGGAAGGA AAACTACTTC GAAATTATAC TCAAAATATA 
GATACCTTGG AGCAGGTTGC AGGAATCCAA AGGATCCTTC AGTGTCATGG TTCCTTTGCA 
ACAGCATCTT GCCTGATTTG TAAATACAAA GTTGATTGTG AAGCTGTTCG TGGAGACATT 
TTTAATCAGG TAGTTCCTCG GTGCCCTAGG TGCCCAGCTG ATGAGCCACT TGCCATCATG 
AAGCCAGAGA TTGTCTTCTT TGGTGAAAAC TTACCAGAAC AGTTTCATAG AGCCATGAAG 
TATGACAAAG ATGAAGTTGA CCTCCTCATT GTTATTGGAT CTTCTCTGAA AGTGAGACCA 
GTAGCACTAA TTCCAAGTTC TATACCCCAT GAAGTGCCTC AAATATTAAT AAATAGGGAA 
CCTTTGCCTC ATCTACATTT TGATGTAGAG CTCCTTGGAG ACTGCGATGT TATAATTAAT 
GAGTTGTGTC ATAGGCTAGG TGGTGAATAT GCCAAACTTT GTTGTAACCC TGTAAAGCTT 
TCAGAAATTA CTGAAAAACC TCCACGCCCA CAAAAGGAAT TGGTTCATTT ATCAGAGTTG 
CCACCAACAC CTCTTCATAT TTCGGAAGAC TCAAGTTCAC CTGAAAGAAC TGTACCACAA 
GACTCTTCTG TGATTGCTAC ACTTGTAGAC CAAGCAACAA ACAACAATGT TAATGATTTA 
GAAGTATCTG AATCAAGTTG TGTGGAAGAA AAACCACAAG AAGTACAGAC TAGTAGGAAT 
GTTGAGAACA TTAATGTGGA AAATCCAGAT TTTAAGGCTG TTGGTTCCAG TACTGCAGAC 
AAAAATGAAA GAACTTCAGT TGCAGAAACA GTGAGAAAAT GCTGGCCTAA TAGACTTGCA 
AAGGAGCAGA TTAGTAAGCG GCTTGAGGGT AATCAATACC TGTTTGTACC ACCAAATCGT 
TACATATTCC ACGGTGCTGA GGTATACTCA GACTCTGAAG ATGACGTCTT GTCCTCTAGT 
TCCTGTGGCA GTAACAGTGA CAGTGGCACA TGCCAGAGTC CAAGTTTAGA AGAACCCTTG 
GAAGATGAAA GTGAAATTGA AGAATTCTAC AATGGCTTGG AAGATGATAC GGAGAGGCCC 
GAATGTGCTG GAGGATCTGG ATTTGGAGCT GATGGAGGGG ATCAAGAGGT TGTTAATGAA 
GCTATAGCTA CAAGACAGGA ATTGACAGAT GTAAACTATC CATCAGACAA ATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Sirt1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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