Pcdhgb8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcdhgb8
Entrez Gene ID	93705
Full Name	protocadherin gamma subfamily B, 8
General protein information	Preferred Names protocadherin gamma subfamily B, 8 Names protocadherin gamma subfamily B, 8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_033580.2	NP_291058.1	protocadherin gamma subfamily B, 8 precursor
NM_033580.3	NP_291058.1	protocadherin gamma subfamily B, 8 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pcdhgb8	NP_291058.1
Rattus norvegicus (Norway rat)	Pcdhgb8	NP_001032236.1
Homo sapiens (human)	PCDHGB3	NP_061747.1

Gene Ontology
Bibliography

Related articles in PubMed

Maturation of postnatally generated olfactory bulb granule cells depends on functional ?-protocadherin expression.
Ledderose J, Dieter S, Schwarz MK
Scientific reports31514(2013)

Functional significance of isoform diversification in the protocadherin gamma gene cluster.
Chen WV, Alvarez FJ, Lefebvre JL, Friedman B, Nwakeze C, Geiman E, Smith C, Thu CA, Tapia JC, Tasic B, Sanes JR, Maniatis T
Neuron75(3)402-9(2012 Aug)

A differential developmental pattern of spinal interneuron apoptosis during synaptogenesis: insights from genetic analyses of the protocadherin-gamma gene cluster.
Prasad T, Wang X, Gray PA, Weiner JA
Development (Cambridge, England)135(24)4153-64(2008 Dec)

The b1 isoform of protocadherin-gamma (Pcdhgamma) interacts with the microtubule-destabilizing protein SCG10.
Gayet O, Labella V, Henderson CE, Kallenbach S
FEBS letters578(1-2)175-9(2004 Dec)

Interaction with protocadherin-gamma regulates the cell surface expression of protocadherin-alpha.
Murata Y, Hamada S, Morishita H, Mutoh T, Yagi T
The Journal of biological chemistry279(47)49508-16(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pcdhgb8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcdhgb8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08808	NM_033580.3 Latest version!	Mus musculus protocadherin gamma subfamily B, 8 (Pcdhgb8), transcript variant 1, coding, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$545.30 $779.00
View Old Accession Versions >>
OMu08808	NM_033580.2	Mus musculus protocadherin gamma subfamily B, 8 (Pcdhgb8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$545.30 $779.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcdhgb8 ( NM_033580.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_291058.1 Mus musculus protocadherin gamma subfamily B, 8 (Pcdhgb8), mRNA.

Price & Availability↑

CloneID	OMu08808
Clone ID Related Accession (Same CDS sequence)	NM_033580.2 , NM_033580.3
Accession Version	NM_033580.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Mus musculus(house mouse)
Product	protocadherin gamma subfamily B, 8 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC139099.1 and BC059927.1. On Jan 8, 2010 this sequence version replaced NM_033580.1. ##Evidence-Data-START## Transcript exon combination :: BC139099.1, AY013810.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCGCA GCAGCGTGAA GCTAGGGAGC AGGACCCCGT GGAGGCAAGT ACTCTTTCCC 
TTCTTGCTGC CTTTGTTCTG CACGGGGCTC TCAGAGCAAG TCCGGTACTC TATTCCGGAA 
GAAATGGCCA TGGGTTCAGT CGTGGGAAAT CTCGCTGAGG ACCTGGGGCT GCCTGTTCAG 
GACTTACTGA CAAGAAACCT TAGAGTTATT GCAGAGAAAC CGTATCTTTC TGTGAACCCA 
GAGAACGGGA ACATAGTTGT GAGCGACAGA ATAGACCGAG AGTTTCTCTG CTTCCAAAGC 
CCTCTGTGCG TCCTGCCCTT AGAGATTGTG GCAGAAAATC CCCTAAACGT TTTTCACGTC 
AGCGTGGTGA TAGAAGATAT AAATGATAAC CCTCCCCGTT TCCTCCAAAA TAGCATTGTT 
TTGCAAATCA ATGAACTTGC GATTCCAGGC ACTAGGTTTG GCCTGGAATC TGCCATAGAT 
GCAGATGTAG GGCTGAACTC TCTCCAGAGT TACCAACTCA GTTTGAATGA GCACTTCTCT 
CTGGTGGTAA AGGACAACAC TGAAGGAAAG GATGCCCCCG AATTAGTGTT GGAGAAGCCT 
CTGGACAGGG AAAAACAGAG CTCCCAGCTC TTGGTCCTAA CCGCAGTAGA TGGGGGAGAA 
CCAGTCCTAA CGGGCACCGC TCAGATTCAG ATCGAGGTCA CTGATGCCAA TGATAACCCC 
CCAGTGTTCA GCCAAAGTAC GTACAAAGTC AGTCTTAGAG AGGACATGGC CGCAGGCACG 
TCTGTGCTAA CAGTGATAGC CACTGACCAG GATGAGGGTG TCAACGCAGA AGTCACCTAC 
TCCTTCAAAT CACTAGGAGA AGATATTAGA GATAAGTTTA TCCTAGATCA TCAAAGTGGA 
GAAATTAAAT CCAAAGGCCC TATTGACTTT GAAACCAAGA GGACTTACAC CATGAACATA 
GAGGCCAAGG ACGGTGGAGG CATGGCCTCA GAATGTAAAG TTGTAGTGGA AATTCTCGAT 
GAGAACGACA ATGCCCCAGA AGTGGTTTTT ACTTCCGTGT CCAATTCTAT AACCGAAGAT 
GCAGAACCGG GGACAGTGGT GGCTCTGTTC AAAACGTATG ATAAAGATTC AGAAGAAAAT 
GGAAGAGTGA GTTGCTTCGT AAAAGAAACG GTTCCTTTTA GGATTGAATC TTCTGCCAGC 
AATTACTATA AGCTTGTAAC AGATGGGATC CTGGACCGGG AGCAGACTCC AGAGTACAAC 
GTCACCATCA TCGCCACTGA TAAAGGCAAG CCCCCTCTCT CTTCCAGCAC AAGCGTTACC 
CTGCATGTTG GGGATATCAA TGACAATGCT CCAGTTTTCC ACCAGACCTC CTATTTGATC 
CAAGTGGCTG AGAACAACCC TCCTGGAGCT TCCATTGCTC AGGTCAGCGC CTTCGACCCG 
GATTTGGGAT CCAATGGTTT CATCTCCTAC TCCATCATAG CCAGCGACCT AGAGCCTAAA 
TCACTGTGGT CCTACGTGTC GGTGAACCAG GATAGTGGCG TGGTGTTCGC GCAGCGCGCC 
TTCGACCACG AACAGCTGCG CTCCTTCCAG CTGACACTGC AGGCGCGCGA CCAGGGAAAG 
CCCTCGCTCA GCGCCAACGT GAGCATGCGG GTGCTGGTGG GTGACCGCAA CGACAACGCG 
CCACGCGTGC TGTATCCCAC ACTCGAGCCC GACGGCTCTG CGCTCTTCGA CATGGTGCCG 
CGTGCTGCCG AGCCCGGATA CCTGGTCACC AAAGTGGTGG CGGTGGATGC AGACTCTGGA 
CACAATGCCT GGCTATCTTA CCACGTGCTG CAGGCCAGCG ATCCCGGGCT CTTCAGCCTG 
GGACTGCGCA CAGGCGAGGT GCGCACAGCG CGTGCCCTGG GAGACAGGGA CTCAGCGCGG 
CAGCGCCTTC TGGTCGCTGT GCGTGATGGT GGACAACCAC CGCTCTCGGC TACAGCCACG 
CTGCACCTCA TCTTCGCTGA CAGCCTGCAG GAGGTCCTGC CAGACCTCAG AGATGAACCT 
TTGCTGTCTG ACTCCCAAAG TGAGCTTCAG TTTTACCTGG TGGTGGCCTT GGCCTTGGTC 
TCAGTGCTCT TTCTTTTCGT TGTGATCCTG GCCATTGTCC TTCGCCTTCG ACAGTCCCAT 
GGTCCTGCTG TCTCAGACTA TTTTCAGTCG GGCCTCTGCT GTAAGACGAG ACCAGAGGTT 
TCTCTCAACT ACAGCGAGGG GACTCTTCCT TATTCCTACA ATCTCTATGT TGCCTCCAAT 
TGTCAAAAGA CAATTTCTCA ATTTCTTACC TTGACTCCAG AAATGGTCCC TCCGCGAGAC 
CTTTGTACGG AAGCTTCTGT AGCAGTGAGT GTCGCTGAGG AGAATAACAA GATAGTCTCG 
GATTCTATTG CTTCTAATCA CCAAGCCCCG CCCAACACTG ACTGGCGTTT CTCTCAAGCC 
CAGAGACCCG GCACGAGCGG ATCCCAAAAT GGTGATGAAA CTGGCACCTG GCCCAACAAC 
CAGTTTGATA CAGAGATGCT GCAAGCCATG ATCTTGGCCT CTGCCAGCGA AGCTGCTGAT 
GGGAGCTCTA CCCTGGGAGG GGGCGCTGGC ACCATGGGTC TGAGCGCTCG ATACGGACCC 
CAGTTTACCC TGCAGCACGT GCCTGACTAC CGCCAGAACG TGTACATCCC TGGCAGCAAT 
GCCACGCTGA CCAATGCCGC TGGCAAACGA GATGGCAAGG CTCCAGCAGG TGGTAATGGC 
AACAAGAAGA AATCGGGCAA GAAAGAGAAG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_291058.1
CDS	79..2874
Translation	MERSSVKLGSRTPWRQVLFPFLLPLFCTGLSEQVRYSIPEEMAMGSVVGNLAEDLGLPVQDLLTRNLRVIAEKPYLSVNPENGNIVVSDRIDREFLCFQSPLCVLPLEIVAENPLNVFHVSVVIEDINDNPPRFLQNSIVLQINELAIPGTRFGLESAIDADVGLNSLQSYQLSLNEHFSLVVKDNTEGKDAPELVLEKPLDREKQSSQLLVLTAVDGGEPVLTGTAQIQIEVTDANDNPPVFSQSTYKVSLREDMAAGTSVLTVIATDQDEGVNAEVTYSFKSLGEDIRDKFILDHQSGEIKSKGPIDFETKRTYTMNIEAKDGGGMASECKVVVEILDENDNAPEVVFTSVSNSITEDAEPGTVVALFKTYDKDSEENGRVSCFVKETVPFRIESSASNYYKLVTDGILDREQTPEYNVTIIATDKGKPPLSSSTSVTLHVGDINDNAPVFHQTSYLIQVAENNPPGASIAQVSAFDPDLGSNGFISYSIIASDLEPKSLWSYVSVNQDSGVVFAQRAFDHEQLRSFQLTLQARDQGKPSLSANVSMRVLVGDRNDNAPRVLYPTLEPDGSALFDMVPRAAEPGYLVTKVVAVDADSGHNAWLSYHVLQASDPGLFSLGLRTGEVRTARALGDRDSARQRLLVAVRDGGQPPLSATATLHLIFADSLQEVLPDLRDEPLLSDSQSELQFYLVVALALVSVLFLFVVILAIVLRLRQSHGPAVSDYFQSGLCCKTRPEVSLNYSEGTLPYSYNLYVASNCQKTISQFLTLTPEMVPPRDLCTEASVAVSVAEENNKIVSDSIASNHQAPPNTDWRFSQAQRPGTSGSQNGDETGTWPNNQFDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQFTLQHVPDYRQNVYIPGSNATLTNAAGKRDGKAPAGGNGNKKKSGKKEKK

Target ORF information:

RefSeq Version	NM_033580.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protocadherin gamma subfamily B, 8 (Pcdhgb8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033580.2

ORF Insert Sequence:

ATGGAGCGCA GCAGCGTGAA GCTAGGGAGC AGGACCCCGT GGAGGCAAGT ACTCTTTCCC 
TTCTTGCTGC CTTTGTTCTG CACGGGGCTC TCAGAGCAAG TCCGGTACTC TATTCCGGAA 
GAAATGGCCA TGGGTTCAGT CGTGGGAAAT CTCGCTGAGG ACCTGGGGCT GCCTGTTCAG 
GACTTACTGA CAAGAAACCT TAGAGTTATT GCAGAGAAAC CGTATCTTTC TGTGAACCCA 
GAGAACGGGA ACATAGTTGT GAGCGACAGA ATAGACCGAG AGTTTCTCTG CTTCCAAAGC 
CCTCTGTGCG TCCTGCCCTT AGAGATTGTG GCAGAAAATC CCCTAAACGT TTTTCACGTC 
AGCGTGGTGA TAGAAGATAT AAATGATAAC CCTCCCCGTT TCCTCCAAAA TAGCATTGTT 
TTGCAAATCA ATGAACTTGC GATTCCAGGC ACTAGGTTTG GCCTGGAATC TGCCATAGAT 
GCAGATGTAG GGCTGAACTC TCTCCAGAGT TACCAACTCA GTTTGAATGA GCACTTCTCT 
CTGGTGGTAA AGGACAACAC TGAAGGAAAG GATGCCCCCG AATTAGTGTT GGAGAAGCCT 
CTGGACAGGG AAAAACAGAG CTCCCAGCTC TTGGTCCTAA CCGCAGTAGA TGGGGGAGAA 
CCAGTCCTAA CGGGCACCGC TCAGATTCAG ATCGAGGTCA CTGATGCCAA TGATAACCCC 
CCAGTGTTCA GCCAAAGTAC GTACAAAGTC AGTCTTAGAG AGGACATGGC CGCAGGCACG 
TCTGTGCTAA CAGTGATAGC CACTGACCAG GATGAGGGTG TCAACGCAGA AGTCACCTAC 
TCCTTCAAAT CACTAGGAGA AGATATTAGA GATAAGTTTA TCCTAGATCA TCAAAGTGGA 
GAAATTAAAT CCAAAGGCCC TATTGACTTT GAAACCAAGA GGACTTACAC CATGAACATA 
GAGGCCAAGG ACGGTGGAGG CATGGCCTCA GAATGTAAAG TTGTAGTGGA AATTCTCGAT 
GAGAACGACA ATGCCCCAGA AGTGGTTTTT ACTTCCGTGT CCAATTCTAT AACCGAAGAT 
GCAGAACCGG GGACAGTGGT GGCTCTGTTC AAAACGTATG ATAAAGATTC AGAAGAAAAT 
GGAAGAGTGA GTTGCTTCGT AAAAGAAACG GTTCCTTTTA GGATTGAATC TTCTGCCAGC 
AATTACTATA AGCTTGTAAC AGATGGGATC CTGGACCGGG AGCAGACTCC AGAGTACAAC 
GTCACCATCA TCGCCACTGA TAAAGGCAAG CCCCCTCTCT CTTCCAGCAC AAGCGTTACC 
CTGCATGTTG GGGATATCAA TGACAATGCT CCAGTTTTCC ACCAGACCTC CTATTTGATC 
CAAGTGGCTG AGAACAACCC TCCTGGAGCT TCCATTGCTC AGGTCAGCGC CTTCGACCCG 
GATTTGGGAT CCAATGGTTT CATCTCCTAC TCCATCATAG CCAGCGACCT AGAGCCTAAA 
TCACTGTGGT CCTACGTGTC GGTGAACCAG GATAGTGGCG TGGTGTTCGC GCAGCGCGCC 
TTCGACCACG AACAGCTGCG CTCCTTCCAG CTGACACTGC AGGCGCGCGA CCAGGGAAAG 
CCCTCGCTCA GCGCCAACGT GAGCATGCGG GTGCTGGTGG GTGACCGCAA CGACAACGCG 
CCACGCGTGC TGTATCCCAC ACTCGAGCCC GACGGCTCTG CGCTCTTCGA CATGGTGCCG 
CGTGCTGCCG AGCCCGGATA CCTGGTCACC AAAGTGGTGG CGGTGGATGC AGACTCTGGA 
CACAATGCCT GGCTATCTTA CCACGTGCTG CAGGCCAGCG ATCCCGGGCT CTTCAGCCTG 
GGACTGCGCA CAGGCGAGGT GCGCACAGCG CGTGCCCTGG GAGACAGGGA CTCAGCGCGG 
CAGCGCCTTC TGGTCGCTGT GCGTGATGGT GGACAACCAC CGCTCTCGGC TACAGCCACG 
CTGCACCTCA TCTTCGCTGA CAGCCTGCAG GAGGTCCTGC CAGACCTCAG AGATGAACCT 
TTGCTGTCTG ACTCCCAAAG TGAGCTTCAG TTTTACCTGG TGGTGGCCTT GGCCTTGGTC 
TCAGTGCTCT TTCTTTTCGT TGTGATCCTG GCCATTGTCC TTCGCCTTCG ACAGTCCCAT 
GGTCCTGCTG TCTCAGACTA TTTTCAGTCG GGCCTCTGCT GTAAGACGAG ACCAGAGGTT 
TCTCTCAACT ACAGCGAGGG GACTCTTCCT TATTCCTACA ATCTCTATGT TGCCTCCAAT 
TGTCAAAAGA CAATTTCTCA ATTTCTTACC TTGACTCCAG AAATGGTCCC TCCGCGAGAC 
CTTTGTACGG AAGCTTCTGT AGCAGTGAGT GTCGCTGAGG AGAATAACAA GATAGTCTCG 
GATTCTATTG CTTCTAATCA CCAAGCCCCG CCCAACACTG ACTGGCGTTT CTCTCAAGCC 
CAGAGACCCG GCACGAGCGG ATCCCAAAAT GGTGATGAAA CTGGCACCTG GCCCAACAAC 
CAGTTTGATA CAGAGATGCT GCAAGCCATG ATCTTGGCCT CTGCCAGCGA AGCTGCTGAT 
GGGAGCTCTA CCCTGGGAGG GGGCGCTGGC ACCATGGGTC TGAGCGCTCG ATACGGACCC 
CAGTTTACCC TGCAGCACGT GCCTGACTAC CGCCAGAACG TGTACATCCC TGGCAGCAAT 
GCCACGCTGA CCAATGCCGC TGGCAAACGA GATGGCAAGG CTCCAGCAGG TGGTAATGGC 
AACAAGAAGA AATCGGGCAA GAAAGAGAAG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcdhgb8 ( NM_033580.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_291058.1 Mus musculus protocadherin gamma subfamily B, 8 (Pcdhgb8), transcript variant 1, coding, mRNA.

Price & Availability↑

CloneID	OMu08808
Clone ID Related Accession (Same CDS sequence)	NM_033580.2 , NM_033580.3
Accession Version	NM_033580.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	protocadherin gamma subfamily B, 8 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC139099.1. On Feb 1, 2019 this sequence version replaced NM_033580.2. Transcript Variant: This variant (1, coding) represents the functional allele that is not present in some mouse strains, including the C57BL/6 strain of the reference genome. ##Evidence-Data-START## Transcript exon combination :: BC139099.1, AY013810.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## polymorphic pseudogene :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGAGCGCA GCAGCGTGAA GCTAGGGAGC AGGACCCCGT GGAGGCAAGT ACTCTTTCCC 
TTCTTGCTGC CTTTGTTCTG CACGGGGCTC TCAGAGCAAG TCCGGTACTC TATTCCGGAA 
GAAATGGCCA TGGGTTCAGT CGTGGGAAAT CTCGCTGAGG ACCTGGGGCT GCCTGTTCAG 
GACTTACTGA CAAGAAACCT TAGAGTTATT GCAGAGAAAC CGTATCTTTC TGTGAACCCA 
GAGAACGGGA ACATAGTTGT GAGCGACAGA ATAGACCGAG AGTTTCTCTG CTTCCAAAGC 
CCTCTGTGCG TCCTGCCCTT AGAGATTGTG GCAGAAAATC CCCTAAACGT TTTTCACGTC 
AGCGTGGTGA TAGAAGATAT AAATGATAAC CCTCCCCGTT TCCTCCAAAA TAGCATTGTT 
TTGCAAATCA ATGAACTTGC GATTCCAGGC ACTAGGTTTG GCCTGGAATC TGCCATAGAT 
GCAGATGTAG GGCTGAACTC TCTCCAGAGT TACCAACTCA GTTTGAATGA GCACTTCTCT 
CTGGTGGTAA AGGACAACAC TGAAGGAAAG GATGCCCCCG AATTAGTGTT GGAGAAGCCT 
CTGGACAGGG AAAAACAGAG CTCCCAGCTC TTGGTCCTAA CCGCAGTAGA TGGGGGAGAA 
CCAGTCCTAA CGGGCACCGC TCAGATTCAG ATCGAGGTCA CTGATGCCAA TGATAACCCC 
CCAGTGTTCA GCCAAAGTAC GTACAAAGTC AGTCTTAGAG AGGACATGGC CGCAGGCACG 
TCTGTGCTAA CAGTGATAGC CACTGACCAG GATGAGGGTG TCAACGCAGA AGTCACCTAC 
TCCTTCAAAT CACTAGGAGA AGATATTAGA GATAAGTTTA TCCTAGATCA TCAAAGTGGA 
GAAATTAAAT CCAAAGGCCC TATTGACTTT GAAACCAAGA GGACTTACAC CATGAACATA 
GAGGCCAAGG ACGGTGGAGG CATGGCCTCA GAATGTAAAG TTGTAGTGGA AATTCTCGAT 
GAGAACGACA ATGCCCCAGA AGTGGTTTTT ACTTCCGTGT CCAATTCTAT AACCGAAGAT 
GCAGAACCGG GGACAGTGGT GGCTCTGTTC AAAACGTATG ATAAAGATTC AGAAGAAAAT 
GGAAGAGTGA GTTGCTTCGT AAAAGAAACG GTTCCTTTTA GGATTGAATC TTCTGCCAGC 
AATTACTATA AGCTTGTAAC AGATGGGATC CTGGACCGGG AGCAGACTCC AGAGTACAAC 
GTCACCATCA TCGCCACTGA TAAAGGCAAG CCCCCTCTCT CTTCCAGCAC AAGCGTTACC 
CTGCATGTTG GGGATATCAA TGACAATGCT CCAGTTTTCC ACCAGACCTC CTATTTGATC 
CAAGTGGCTG AGAACAACCC TCCTGGAGCT TCCATTGCTC AGGTCAGCGC CTTCGACCCG 
GATTTGGGAT CCAATGGTTT CATCTCCTAC TCCATCATAG CCAGCGACCT AGAGCCTAAA 
TCACTGTGGT CCTACGTGTC GGTGAACCAG GATAGTGGCG TGGTGTTCGC GCAGCGCGCC 
TTCGACCACG AACAGCTGCG CTCCTTCCAG CTGACACTGC AGGCGCGCGA CCAGGGAAAG 
CCCTCGCTCA GCGCCAACGT GAGCATGCGG GTGCTGGTGG GTGACCGCAA CGACAACGCG 
CCACGCGTGC TGTATCCCAC ACTCGAGCCC GACGGCTCTG CGCTCTTCGA CATGGTGCCG 
CGTGCTGCCG AGCCCGGATA CCTGGTCACC AAAGTGGTGG CGGTGGATGC AGACTCTGGA 
CACAATGCCT GGCTATCTTA CCACGTGCTG CAGGCCAGCG ATCCCGGGCT CTTCAGCCTG 
GGACTGCGCA CAGGCGAGGT GCGCACAGCG CGTGCCCTGG GAGACAGGGA CTCAGCGCGG 
CAGCGCCTTC TGGTCGCTGT GCGTGATGGT GGACAACCAC CGCTCTCGGC TACAGCCACG 
CTGCACCTCA TCTTCGCTGA CAGCCTGCAG GAGGTCCTGC CAGACCTCAG AGATGAACCT 
TTGCTGTCTG ACTCCCAAAG TGAGCTTCAG TTTTACCTGG TGGTGGCCTT GGCCTTGGTC 
TCAGTGCTCT TTCTTTTCGT TGTGATCCTG GCCATTGTCC TTCGCCTTCG ACAGTCCCAT 
GGTCCTGCTG TCTCAGACTA TTTTCAGTCG GGCCTCTGCT GTAAGACGAG ACCAGAGGTT 
TCTCTCAACT ACAGCGAGGG GACTCTTCCT TATTCCTACA ATCTCTATGT TGCCTCCAAT 
TGTCAAAAGA CAATTTCTCA ATTTCTTACC TTGACTCCAG AAATGGTCCC TCCGCGAGAC 
CTTTGTACGG AAGCTTCTGT AGCAGTGAGT GTCGCTGAGG AGAATAACAA GATAGTCTCG 
GATTCTATTG CTTCTAATCA CCAAGCCCCG CCCAACACTG ACTGGCGTTT CTCTCAAGCC 
CAGAGACCCG GCACGAGCGG ATCCCAAAAT GGTGATGAAA CTGGCACCTG GCCCAACAAC 
CAGTTTGATA CAGAGATGCT GCAAGCCATG ATCTTGGCCT CTGCCAGCGA AGCTGCTGAT 
GGGAGCTCTA CCCTGGGAGG GGGCGCTGGC ACCATGGGTC TGAGCGCTCG ATACGGACCC 
CAGTTTACCC TGCAGCACGT GCCTGACTAC CGCCAGAACG TGTACATCCC TGGCAGCAAT 
GCCACGCTGA CCAATGCCGC TGGCAAACGA GATGGCAAGG CTCCAGCAGG TGGTAATGGC 
AACAAGAAGA AATCGGGCAA GAAAGAGAAG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_291058.1
CDS	79..2874
Translation	MERSSVKLGSRTPWRQVLFPFLLPLFCTGLSEQVRYSIPEEMAMGSVVGNLAEDLGLPVQDLLTRNLRVIAEKPYLSVNPENGNIVVSDRIDREFLCFQSPLCVLPLEIVAENPLNVFHVSVVIEDINDNPPRFLQNSIVLQINELAIPGTRFGLESAIDADVGLNSLQSYQLSLNEHFSLVVKDNTEGKDAPELVLEKPLDREKQSSQLLVLTAVDGGEPVLTGTAQIQIEVTDANDNPPVFSQSTYKVSLREDMAAGTSVLTVIATDQDEGVNAEVTYSFKSLGEDIRDKFILDHQSGEIKSKGPIDFETKRTYTMNIEAKDGGGMASECKVVVEILDENDNAPEVVFTSVSNSITEDAEPGTVVALFKTYDKDSEENGRVSCFVKETVPFRIESSASNYYKLVTDGILDREQTPEYNVTIIATDKGKPPLSSSTSVTLHVGDINDNAPVFHQTSYLIQVAENNPPGASIAQVSAFDPDLGSNGFISYSIIASDLEPKSLWSYVSVNQDSGVVFAQRAFDHEQLRSFQLTLQARDQGKPSLSANVSMRVLVGDRNDNAPRVLYPTLEPDGSALFDMVPRAAEPGYLVTKVVAVDADSGHNAWLSYHVLQASDPGLFSLGLRTGEVRTARALGDRDSARQRLLVAVRDGGQPPLSATATLHLIFADSLQEVLPDLRDEPLLSDSQSELQFYLVVALALVSVLFLFVVILAIVLRLRQSHGPAVSDYFQSGLCCKTRPEVSLNYSEGTLPYSYNLYVASNCQKTISQFLTLTPEMVPPRDLCTEASVAVSVAEENNKIVSDSIASNHQAPPNTDWRFSQAQRPGTSGSQNGDETGTWPNNQFDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQFTLQHVPDYRQNVYIPGSNATLTNAAGKRDGKAPAGGNGNKKKSGKKEKK

Target ORF information:

RefSeq Version	NM_033580.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protocadherin gamma subfamily B, 8 (Pcdhgb8), transcript variant 1, coding, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033580.3

ORF Insert Sequence:

ATGGAGCGCA GCAGCGTGAA GCTAGGGAGC AGGACCCCGT GGAGGCAAGT ACTCTTTCCC 
TTCTTGCTGC CTTTGTTCTG CACGGGGCTC TCAGAGCAAG TCCGGTACTC TATTCCGGAA 
GAAATGGCCA TGGGTTCAGT CGTGGGAAAT CTCGCTGAGG ACCTGGGGCT GCCTGTTCAG 
GACTTACTGA CAAGAAACCT TAGAGTTATT GCAGAGAAAC CGTATCTTTC TGTGAACCCA 
GAGAACGGGA ACATAGTTGT GAGCGACAGA ATAGACCGAG AGTTTCTCTG CTTCCAAAGC 
CCTCTGTGCG TCCTGCCCTT AGAGATTGTG GCAGAAAATC CCCTAAACGT TTTTCACGTC 
AGCGTGGTGA TAGAAGATAT AAATGATAAC CCTCCCCGTT TCCTCCAAAA TAGCATTGTT 
TTGCAAATCA ATGAACTTGC GATTCCAGGC ACTAGGTTTG GCCTGGAATC TGCCATAGAT 
GCAGATGTAG GGCTGAACTC TCTCCAGAGT TACCAACTCA GTTTGAATGA GCACTTCTCT 
CTGGTGGTAA AGGACAACAC TGAAGGAAAG GATGCCCCCG AATTAGTGTT GGAGAAGCCT 
CTGGACAGGG AAAAACAGAG CTCCCAGCTC TTGGTCCTAA CCGCAGTAGA TGGGGGAGAA 
CCAGTCCTAA CGGGCACCGC TCAGATTCAG ATCGAGGTCA CTGATGCCAA TGATAACCCC 
CCAGTGTTCA GCCAAAGTAC GTACAAAGTC AGTCTTAGAG AGGACATGGC CGCAGGCACG 
TCTGTGCTAA CAGTGATAGC CACTGACCAG GATGAGGGTG TCAACGCAGA AGTCACCTAC 
TCCTTCAAAT CACTAGGAGA AGATATTAGA GATAAGTTTA TCCTAGATCA TCAAAGTGGA 
GAAATTAAAT CCAAAGGCCC TATTGACTTT GAAACCAAGA GGACTTACAC CATGAACATA 
GAGGCCAAGG ACGGTGGAGG CATGGCCTCA GAATGTAAAG TTGTAGTGGA AATTCTCGAT 
GAGAACGACA ATGCCCCAGA AGTGGTTTTT ACTTCCGTGT CCAATTCTAT AACCGAAGAT 
GCAGAACCGG GGACAGTGGT GGCTCTGTTC AAAACGTATG ATAAAGATTC AGAAGAAAAT 
GGAAGAGTGA GTTGCTTCGT AAAAGAAACG GTTCCTTTTA GGATTGAATC TTCTGCCAGC 
AATTACTATA AGCTTGTAAC AGATGGGATC CTGGACCGGG AGCAGACTCC AGAGTACAAC 
GTCACCATCA TCGCCACTGA TAAAGGCAAG CCCCCTCTCT CTTCCAGCAC AAGCGTTACC 
CTGCATGTTG GGGATATCAA TGACAATGCT CCAGTTTTCC ACCAGACCTC CTATTTGATC 
CAAGTGGCTG AGAACAACCC TCCTGGAGCT TCCATTGCTC AGGTCAGCGC CTTCGACCCG 
GATTTGGGAT CCAATGGTTT CATCTCCTAC TCCATCATAG CCAGCGACCT AGAGCCTAAA 
TCACTGTGGT CCTACGTGTC GGTGAACCAG GATAGTGGCG TGGTGTTCGC GCAGCGCGCC 
TTCGACCACG AACAGCTGCG CTCCTTCCAG CTGACACTGC AGGCGCGCGA CCAGGGAAAG 
CCCTCGCTCA GCGCCAACGT GAGCATGCGG GTGCTGGTGG GTGACCGCAA CGACAACGCG 
CCACGCGTGC TGTATCCCAC ACTCGAGCCC GACGGCTCTG CGCTCTTCGA CATGGTGCCG 
CGTGCTGCCG AGCCCGGATA CCTGGTCACC AAAGTGGTGG CGGTGGATGC AGACTCTGGA 
CACAATGCCT GGCTATCTTA CCACGTGCTG CAGGCCAGCG ATCCCGGGCT CTTCAGCCTG 
GGACTGCGCA CAGGCGAGGT GCGCACAGCG CGTGCCCTGG GAGACAGGGA CTCAGCGCGG 
CAGCGCCTTC TGGTCGCTGT GCGTGATGGT GGACAACCAC CGCTCTCGGC TACAGCCACG 
CTGCACCTCA TCTTCGCTGA CAGCCTGCAG GAGGTCCTGC CAGACCTCAG AGATGAACCT 
TTGCTGTCTG ACTCCCAAAG TGAGCTTCAG TTTTACCTGG TGGTGGCCTT GGCCTTGGTC 
TCAGTGCTCT TTCTTTTCGT TGTGATCCTG GCCATTGTCC TTCGCCTTCG ACAGTCCCAT 
GGTCCTGCTG TCTCAGACTA TTTTCAGTCG GGCCTCTGCT GTAAGACGAG ACCAGAGGTT 
TCTCTCAACT ACAGCGAGGG GACTCTTCCT TATTCCTACA ATCTCTATGT TGCCTCCAAT 
TGTCAAAAGA CAATTTCTCA ATTTCTTACC TTGACTCCAG AAATGGTCCC TCCGCGAGAC 
CTTTGTACGG AAGCTTCTGT AGCAGTGAGT GTCGCTGAGG AGAATAACAA GATAGTCTCG 
GATTCTATTG CTTCTAATCA CCAAGCCCCG CCCAACACTG ACTGGCGTTT CTCTCAAGCC 
CAGAGACCCG GCACGAGCGG ATCCCAAAAT GGTGATGAAA CTGGCACCTG GCCCAACAAC 
CAGTTTGATA CAGAGATGCT GCAAGCCATG ATCTTGGCCT CTGCCAGCGA AGCTGCTGAT 
GGGAGCTCTA CCCTGGGAGG GGGCGCTGGC ACCATGGGTC TGAGCGCTCG ATACGGACCC 
CAGTTTACCC TGCAGCACGT GCCTGACTAC CGCCAGAACG TGTACATCCC TGGCAGCAAT 
GCCACGCTGA CCAATGCCGC TGGCAAACGA GATGGCAAGG CTCCAGCAGG TGGTAATGGC 
AACAAGAAGA AATCGGGCAA GAAAGAGAAG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Maturation of postnatally generated olfactory bulb granule cells depends on functional ?-protocadherin expression.
Scientific reports31514(2013)
Ledderose J,Dieter S,Schwarz MK

Functional significance of isoform diversification in the protocadherin gamma gene cluster.
Neuron75(3)402-9(2012 Aug)
Chen WV,Alvarez FJ,Lefebvre JL,Friedman B,Nwakeze C,Geiman E,Smith C,Thu CA,Tapia JC,Tasic B,Sanes JR,Maniatis T

A differential developmental pattern of spinal interneuron apoptosis during synaptogenesis: insights from genetic analyses of the protocadherin-gamma gene cluster.
Development (Cambridge, England)135(24)4153-64(2008 Dec)
Prasad T,Wang X,Gray PA,Weiner JA

The b1 isoform of protocadherin-gamma (Pcdhgamma) interacts with the microtubule-destabilizing protein SCG10.
FEBS letters578(1-2)175-9(2004 Dec)
Gayet O,Labella V,Henderson CE,Kallenbach S

Interaction with protocadherin-gamma regulates the cell surface expression of protocadherin-alpha.
The Journal of biological chemistry279(47)49508-16(2004 Nov)
Murata Y,Hamada S,Morishita H,Mutoh T,Yagi T

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Pcdhgb8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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