Pcdhgb1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcdhgb1
Entrez Gene ID	93699
Full Name	protocadherin gamma subfamily B, 1
General protein information	Preferred Names protocadherin gamma subfamily B, 1 Names protocadherin gamma-B1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_033574.3	NP_291052.3	protocadherin gamma-B1 precursor
NM_033574.4	NP_291052.3	protocadherin gamma-B1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	PCDHGB1	NP_061745.1
Pan troglodytes (chimpanzee)	PCDHGB1	NP_001076038.1
Mus musculus (house mouse)	Pcdhgb1	NP_291052.3

Gene Ontology
Bibliography

Related articles in PubMed

Maturation of postnatally generated olfactory bulb granule cells depends on functional ?-protocadherin expression.
Ledderose J, Dieter S, Schwarz MK
Scientific reports31514(2013)

Functional significance of isoform diversification in the protocadherin gamma gene cluster.
Chen WV, Alvarez FJ, Lefebvre JL, Friedman B, Nwakeze C, Geiman E, Smith C, Thu CA, Tapia JC, Tasic B, Sanes JR, Maniatis T
Neuron75(3)402-9(2012 Aug)

Combinatorial homophilic interaction between gamma-protocadherin multimers greatly expands the molecular diversity of cell adhesion.
Schreiner D, Weiner JA
Proceedings of the National Academy of Sciences of the United States of America107(33)14893-8(2010 Aug)

A differential developmental pattern of spinal interneuron apoptosis during synaptogenesis: insights from genetic analyses of the protocadherin-gamma gene cluster.
Prasad T, Wang X, Gray PA, Weiner JA
Development (Cambridge, England)135(24)4153-64(2008 Dec)

The b1 isoform of protocadherin-gamma (Pcdhgamma) interacts with the microtubule-destabilizing protein SCG10.
Gayet O, Labella V, Henderson CE, Kallenbach S
FEBS letters578(1-2)175-9(2004 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data demonstrate role of the gamma-Pcdhs in synaptogenesis and implications for the molecular control of neuronal specificity.
Title: Combinatorial homophilic interaction between gamma-protocadherin multimers greatly expands the molecular diversity of cell adhesion.

Results identify SCG10 as a cytoplasmic interactor for protocadherin-gamma (Pcdhgamma)-b1 and other isoforms of the Pcdhgamma-b subfamily, and show that SCG10 and Pcdhgamma-b1 are found together in certain neuronal growth cones.
Title: The b1 isoform of protocadherin-gamma (Pcdhgamma) interacts with the microtubule-destabilizing protein SCG10.

The following Pcdhgb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcdhgb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08791	NM_033574.4 Latest version!	Mus musculus protocadherin gamma subfamily B, 1 (Pcdhgb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
View Old Accession Versions >>
OMu08791	NM_033574.3	Mus musculus protocadherin gamma subfamily B, 1 (Pcdhgb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcdhgb1 ( NM_033574.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_291052.3 Mus musculus protocadherin gamma subfamily B, 1 (Pcdhgb1), mRNA.

Price & Availability↑

CloneID	OMu08791
Clone ID Related Accession (Same CDS sequence)	NM_033574.3 , NM_033574.4
Accession Version	NM_033574.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Mus musculus(house mouse)
Product	protocadherin gamma-B1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY013804.1. On Jun 10, 2007 this sequence version replaced NM_033574.2. ##Evidence-Data-START## Transcript exon combination :: AY013804.1, BC054741.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAAAATC AGGTATTGCT TCACTTCCTG GTGCCTTTGT TCTGCGGCGC CCTCTCCCAG 
CCCATCCACT ACTCCATTCC GGAGGAGCTA GCCAAGGGCT CTCTAGTGGG GAGTCTCGCC 
AAGGATCTGG GACTCAGTAT CCAAGAATTG CCAGCTCGAA AACTGAGAGT TAGTGCAGAG 
GATTATTTCA GCGTTAGTGC AGAGAGTGGG GATTTGTTAG TGAGCGGCAG GATAGACAGA 
GAAAAGATTT GTGGGAGGAA ATCTGAGTGT GCACTGGAAT TTGAATCTGT TACTGAAAAC 
CCGATGAACA TTTTCCACGT AACGGTTGCC ATCCAAGACA TTAACGATAA TGCACCACAT 
TTCTTTGGGA AAAGCATTGA ATTGGAGATC TGTGAGTCAG CCTTAGCAGG GGCAAAATTC 
CCTCTGGACT CTGCGCGAGA TGCAGATGTG GGAAGCAACT CCCTGAAGAT GTACACTGTC 
AGCCCCAATC CACACTTCTC CTTGTCAACG AAGGAAAGTC CTGATGGAAG TAAATACCCA 
GAACTGCTGC TGGAAACCCC TCTGGACAGG GAGCATCAGA GCTCCCATCA CTTAATACTG 
ACCGCCATGG ATGGTGGAGA CCCGCCCCTA AGCAGCACCA CCCAGGTCTG GATCAAAGTC 
ACCGACGCCA ATGACAATGC TCCAGTGTTC AGCCAGGACA CTTACAAAGT TAGCCTTCGT 
GAAAACATAC CCCCAGGAAC GTTAGTGCTG CAAGTGACAG CTTCTGATCA GGATGAGAGT 
ATTCACGGAG AGATCACCTA TGCCTTCCTT AATGCCCCAG CAAGTACTAA CCTTGTCTTC 
AATCTCAATC CAAATACTGG AGCTATCACA ACTAATGGTA CATTGGATTT TGAAGAAAAG 
AATAGATACA CATTGGGTGT AGAAGCCAAG GACGGAGGGG TGCACACGGC TCACTGTAAT 
GTTCAGATCG AAATTCTTGA TGACAACGAC AATGCCCCAG AGGTGACATT CATGTCCTTT 
TCTAACCAAG TTCCAGAGGA TTCAGTCCTT GGAACAGTAA TCGCCCTCAT TAAAGTTCGA 
GACAAAGATT CTGGACAAAA TGGTTTGGTG GCATGCCATA TTCAGGAAGA ACTTCCCTTC 
AAATTACAAC CCACCTCCAA GAATTATTAC AAGTTAGTGA TTGACAAGGC CCTAAACCGA 
GAACAGACCC CAGAGTACAA CGTCACTATC ACAGCTACTG ACAGTGGCAA GCCCTCGCTT 
TCCTCCAAGA CATGTGTTAC TGTACACATC ACAGATATCA ACGACAATGC ACCGGTTTTT 
CACCAGGCCT CCTACTTGGT CCACGTGGCT GAGAACAACC CACCTGGAGC CTCTATTGCT 
CAAGTCAGCG CCTCAGATCC GGATTTGGGA TCCAATGGCC TCATTTCCTA CTCCATCATA 
GCCAGCGACC TAGAGCCAAG GGCACTATCG TCCTTCGTGT CCGTGAACCA GGACAGTGGA 
GTGGTGTTCG CGCAGCGCGC CTTCGACCAC GAGCAGCTGC GTTCCTTCCA GCTGACACTG 
CAGGCGCGGG ACCACGGATC GCCCACACTC AGTGCCAATG TGAGCATGCG CGTGTTGGTG 
GGTGACCGCA ACGACAACGC ACCACGCGTG CTGTATCCCA CACTTGAGCC CGACGGCTCT 
GCGCTCTTCG ACATGGTGCC GCGCGCTGCC GAGCCCGGAT ACCTGGTCAC CAAGGTGGTG 
GCTGTGGACG CAGACTCCGG ACACAATGCC TGGCTGTCTT ACCACGTGCT GCAGGCCAGC 
GATCCCGGGC TCTTCAGCCT GGGACTGCGC ACAGGCGAGG TGCGCACAGC GCGTGCCCTG 
GGAGACAGGG ACTCAGCGCG GCAGCGCCTT CTGGTCGCTG TGCGTGATGG TGGACAGCCA 
CCGCTCTCCG CTACAGCCAC GCTGCACCTC ATCTTCGCTG ACAGCCTGCA GGAGGTCCTG 
CCAGACCTCA GCGATGACCC TCTACCATCT GACCCTCAGA GTGAGCTTCA GTTTTACCTG 
GTGGTGGCCT TGGCCCTGAT CTCAGTGCTC TTCCTCCTGG CGGTGATTCT GGCTATTGCC 
CTGCGTCTGA GACACTCCTC CAGATCAGCT GCCTGGGGCT GCTTTCAGCC TGGCCTCAGC 
CTGAAGTCTC AACCTGTGGT TCCTCCCAAC TACAATGAGG GGACATTACC CTATTCCTAT 
AATCTCTGCG TTGCTTCACA TTCTACGACT ATGGCAGGGT TTAATTTTCA CCACGTGGCA 
CCAGAGGTTG CTCCCCCTCA GGATCTCCTG TGCGATGATC CTTCTGTGGT TGTATGTGGC 
AATAATGAAG ATCCCCAAGT CTCGGACGAC TCTGCTTTCC AGCAAGCCCC GCCCAACACT 
GACTGGCGTT TCTCTCAAGC CCAGAGACCC GGCACGAGCG GATCCCAAAA TGGTGATGAA 
ACTGGCACCT GGCCCAACAA CCAGTTTGAT ACAGAGATGC TGCAAGCCAT GATCTTGGCC 
TCTGCCAGCG AAGCTGCTGA TGGGAGCTCT ACCCTGGGAG GGGGCGCTGG CACCATGGGT 
CTGAGCGCTC GATACGGACC CCAGTTTACC CTGCAGCACG TGCCTGACTA CCGCCAGAAC 
GTGTACATCC CTGGCAGCAA TGCCACGCTG ACCAATGCCG CTGGCAAACG AGATGGCAAG 
GCTCCAGCAG GTGGTAATGG CAACAAGAAG AAATCGGGCA AGAAAGAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_291052.3
CDS	1..2757
Translation	MENQVLLHFLVPLFCGALSQPIHYSIPEELAKGSLVGSLAKDLGLSIQELPARKLRVSAEDYFSVSAESGDLLVSGRIDREKICGRKSECALEFESVTENPMNIFHVTVAIQDINDNAPHFFGKSIELEICESALAGAKFPLDSARDADVGSNSLKMYTVSPNPHFSLSTKESPDGSKYPELLLETPLDREHQSSHHLILTAMDGGDPPLSSTTQVWIKVTDANDNAPVFSQDTYKVSLRENIPPGTLVLQVTASDQDESIHGEITYAFLNAPASTNLVFNLNPNTGAITTNGTLDFEEKNRYTLGVEAKDGGVHTAHCNVQIEILDDNDNAPEVTFMSFSNQVPEDSVLGTVIALIKVRDKDSGQNGLVACHIQEELPFKLQPTSKNYYKLVIDKALNREQTPEYNVTITATDSGKPSLSSKTCVTVHITDINDNAPVFHQASYLVHVAENNPPGASIAQVSASDPDLGSNGLISYSIIASDLEPRALSSFVSVNQDSGVVFAQRAFDHEQLRSFQLTLQARDHGSPTLSANVSMRVLVGDRNDNAPRVLYPTLEPDGSALFDMVPRAAEPGYLVTKVVAVDADSGHNAWLSYHVLQASDPGLFSLGLRTGEVRTARALGDRDSARQRLLVAVRDGGQPPLSATATLHLIFADSLQEVLPDLSDDPLPSDPQSELQFYLVVALALISVLFLLAVILAIALRLRHSSRSAAWGCFQPGLSLKSQPVVPPNYNEGTLPYSYNLCVASHSTTMAGFNFHHVAPEVAPPQDLLCDDPSVVVCGNNEDPQVSDDSAFQQAPPNTDWRFSQAQRPGTSGSQNGDETGTWPNNQFDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQFTLQHVPDYRQNVYIPGSNATLTNAAGKRDGKAPAGGNGNKKKSGKKEKK

Target ORF information:

RefSeq Version	NM_033574.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protocadherin gamma subfamily B, 1 (Pcdhgb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033574.3

ORF Insert Sequence:

ATGGAAAATC AGGTATTGCT TCACTTCCTG GTGCCTTTGT TCTGCGGCGC CCTCTCCCAG 
CCCATCCACT ACTCCATTCC GGAGGAGCTA GCCAAGGGCT CTCTAGTGGG GAGTCTCGCC 
AAGGATCTGG GACTCAGTAT CCAAGAATTG CCAGCTCGAA AACTGAGAGT TAGTGCAGAG 
GATTATTTCA GCGTTAGTGC AGAGAGTGGG GATTTGTTAG TGAGCGGCAG GATAGACAGA 
GAAAAGATTT GTGGGAGGAA ATCTGAGTGT GCACTGGAAT TTGAATCTGT TACTGAAAAC 
CCGATGAACA TTTTCCACGT AACGGTTGCC ATCCAAGACA TTAACGATAA TGCACCACAT 
TTCTTTGGGA AAAGCATTGA ATTGGAGATC TGTGAGTCAG CCTTAGCAGG GGCAAAATTC 
CCTCTGGACT CTGCGCGAGA TGCAGATGTG GGAAGCAACT CCCTGAAGAT GTACACTGTC 
AGCCCCAATC CACACTTCTC CTTGTCAACG AAGGAAAGTC CTGATGGAAG TAAATACCCA 
GAACTGCTGC TGGAAACCCC TCTGGACAGG GAGCATCAGA GCTCCCATCA CTTAATACTG 
ACCGCCATGG ATGGTGGAGA CCCGCCCCTA AGCAGCACCA CCCAGGTCTG GATCAAAGTC 
ACCGACGCCA ATGACAATGC TCCAGTGTTC AGCCAGGACA CTTACAAAGT TAGCCTTCGT 
GAAAACATAC CCCCAGGAAC GTTAGTGCTG CAAGTGACAG CTTCTGATCA GGATGAGAGT 
ATTCACGGAG AGATCACCTA TGCCTTCCTT AATGCCCCAG CAAGTACTAA CCTTGTCTTC 
AATCTCAATC CAAATACTGG AGCTATCACA ACTAATGGTA CATTGGATTT TGAAGAAAAG 
AATAGATACA CATTGGGTGT AGAAGCCAAG GACGGAGGGG TGCACACGGC TCACTGTAAT 
GTTCAGATCG AAATTCTTGA TGACAACGAC AATGCCCCAG AGGTGACATT CATGTCCTTT 
TCTAACCAAG TTCCAGAGGA TTCAGTCCTT GGAACAGTAA TCGCCCTCAT TAAAGTTCGA 
GACAAAGATT CTGGACAAAA TGGTTTGGTG GCATGCCATA TTCAGGAAGA ACTTCCCTTC 
AAATTACAAC CCACCTCCAA GAATTATTAC AAGTTAGTGA TTGACAAGGC CCTAAACCGA 
GAACAGACCC CAGAGTACAA CGTCACTATC ACAGCTACTG ACAGTGGCAA GCCCTCGCTT 
TCCTCCAAGA CATGTGTTAC TGTACACATC ACAGATATCA ACGACAATGC ACCGGTTTTT 
CACCAGGCCT CCTACTTGGT CCACGTGGCT GAGAACAACC CACCTGGAGC CTCTATTGCT 
CAAGTCAGCG CCTCAGATCC GGATTTGGGA TCCAATGGCC TCATTTCCTA CTCCATCATA 
GCCAGCGACC TAGAGCCAAG GGCACTATCG TCCTTCGTGT CCGTGAACCA GGACAGTGGA 
GTGGTGTTCG CGCAGCGCGC CTTCGACCAC GAGCAGCTGC GTTCCTTCCA GCTGACACTG 
CAGGCGCGGG ACCACGGATC GCCCACACTC AGTGCCAATG TGAGCATGCG CGTGTTGGTG 
GGTGACCGCA ACGACAACGC ACCACGCGTG CTGTATCCCA CACTTGAGCC CGACGGCTCT 
GCGCTCTTCG ACATGGTGCC GCGCGCTGCC GAGCCCGGAT ACCTGGTCAC CAAGGTGGTG 
GCTGTGGACG CAGACTCCGG ACACAATGCC TGGCTGTCTT ACCACGTGCT GCAGGCCAGC 
GATCCCGGGC TCTTCAGCCT GGGACTGCGC ACAGGCGAGG TGCGCACAGC GCGTGCCCTG 
GGAGACAGGG ACTCAGCGCG GCAGCGCCTT CTGGTCGCTG TGCGTGATGG TGGACAGCCA 
CCGCTCTCCG CTACAGCCAC GCTGCACCTC ATCTTCGCTG ACAGCCTGCA GGAGGTCCTG 
CCAGACCTCA GCGATGACCC TCTACCATCT GACCCTCAGA GTGAGCTTCA GTTTTACCTG 
GTGGTGGCCT TGGCCCTGAT CTCAGTGCTC TTCCTCCTGG CGGTGATTCT GGCTATTGCC 
CTGCGTCTGA GACACTCCTC CAGATCAGCT GCCTGGGGCT GCTTTCAGCC TGGCCTCAGC 
CTGAAGTCTC AACCTGTGGT TCCTCCCAAC TACAATGAGG GGACATTACC CTATTCCTAT 
AATCTCTGCG TTGCTTCACA TTCTACGACT ATGGCAGGGT TTAATTTTCA CCACGTGGCA 
CCAGAGGTTG CTCCCCCTCA GGATCTCCTG TGCGATGATC CTTCTGTGGT TGTATGTGGC 
AATAATGAAG ATCCCCAAGT CTCGGACGAC TCTGCTTTCC AGCAAGCCCC GCCCAACACT 
GACTGGCGTT TCTCTCAAGC CCAGAGACCC GGCACGAGCG GATCCCAAAA TGGTGATGAA 
ACTGGCACCT GGCCCAACAA CCAGTTTGAT ACAGAGATGC TGCAAGCCAT GATCTTGGCC 
TCTGCCAGCG AAGCTGCTGA TGGGAGCTCT ACCCTGGGAG GGGGCGCTGG CACCATGGGT 
CTGAGCGCTC GATACGGACC CCAGTTTACC CTGCAGCACG TGCCTGACTA CCGCCAGAAC 
GTGTACATCC CTGGCAGCAA TGCCACGCTG ACCAATGCCG CTGGCAAACG AGATGGCAAG 
GCTCCAGCAG GTGGTAATGG CAACAAGAAG AAATCGGGCA AGAAAGAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcdhgb1 ( NM_033574.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_291052.3 Mus musculus protocadherin gamma subfamily B, 1 (Pcdhgb1), mRNA.

Price & Availability↑

CloneID	OMu08791
Clone ID Related Accession (Same CDS sequence)	NM_033574.3 , NM_033574.4
Accession Version	NM_033574.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	protocadherin gamma-B1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC020971.3 and AC129315.3. On Feb 22, 2019 this sequence version replaced NM_033574.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AY013804.1, SRR7652917.388368.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAAAATC AGGTATTGCT TCACTTCCTG GTGCCTTTGT TCTGCGGCGC CCTCTCCCAG 
CCCATCCACT ACTCCATTCC GGAGGAGCTA GCCAAGGGCT CTCTAGTGGG GAGTCTCGCC 
AAGGATCTGG GACTCAGTAT CCAAGAATTG CCAGCTCGAA AACTGAGAGT TAGTGCAGAG 
GATTATTTCA GCGTTAGTGC AGAGAGTGGG GATTTGTTAG TGAGCGGCAG GATAGACAGA 
GAAAAGATTT GTGGGAGGAA ATCTGAGTGT GCACTGGAAT TTGAATCTGT TACTGAAAAC 
CCGATGAACA TTTTCCACGT AACGGTTGCC ATCCAAGACA TTAACGATAA TGCACCACAT 
TTCTTTGGGA AAAGCATTGA ATTGGAGATC TGTGAGTCAG CCTTAGCAGG GGCAAAATTC 
CCTCTGGACT CTGCGCGAGA TGCAGATGTG GGAAGCAACT CCCTGAAGAT GTACACTGTC 
AGCCCCAATC CACACTTCTC CTTGTCAACG AAGGAAAGTC CTGATGGAAG TAAATACCCA 
GAACTGCTGC TGGAAACCCC TCTGGACAGG GAGCATCAGA GCTCCCATCA CTTAATACTG 
ACCGCCATGG ATGGTGGAGA CCCGCCCCTA AGCAGCACCA CCCAGGTCTG GATCAAAGTC 
ACCGACGCCA ATGACAATGC TCCAGTGTTC AGCCAGGACA CTTACAAAGT TAGCCTTCGT 
GAAAACATAC CCCCAGGAAC GTTAGTGCTG CAAGTGACAG CTTCTGATCA GGATGAGAGT 
ATTCACGGAG AGATCACCTA TGCCTTCCTT AATGCCCCAG CAAGTACTAA CCTTGTCTTC 
AATCTCAATC CAAATACTGG AGCTATCACA ACTAATGGTA CATTGGATTT TGAAGAAAAG 
AATAGATACA CATTGGGTGT AGAAGCCAAG GACGGAGGGG TGCACACGGC TCACTGTAAT 
GTTCAGATCG AAATTCTTGA TGACAACGAC AATGCCCCAG AGGTGACATT CATGTCCTTT 
TCTAACCAAG TTCCAGAGGA TTCAGTCCTT GGAACAGTAA TCGCCCTCAT TAAAGTTCGA 
GACAAAGATT CTGGACAAAA TGGTTTGGTG GCATGCCATA TTCAGGAAGA ACTTCCCTTC 
AAATTACAAC CCACCTCCAA GAATTATTAC AAGTTAGTGA TTGACAAGGC CCTAAACCGA 
GAACAGACCC CAGAGTACAA CGTCACTATC ACAGCTACTG ACAGTGGCAA GCCCTCGCTT 
TCCTCCAAGA CATGTGTTAC TGTACACATC ACAGATATCA ACGACAATGC ACCGGTTTTT 
CACCAGGCCT CCTACTTGGT CCACGTGGCT GAGAACAACC CACCTGGAGC CTCTATTGCT 
CAAGTCAGCG CCTCAGATCC GGATTTGGGA TCCAATGGCC TCATTTCCTA CTCCATCATA 
GCCAGCGACC TAGAGCCAAG GGCACTATCG TCCTTCGTGT CCGTGAACCA GGACAGTGGA 
GTGGTGTTCG CGCAGCGCGC CTTCGACCAC GAGCAGCTGC GTTCCTTCCA GCTGACACTG 
CAGGCGCGGG ACCACGGATC GCCCACACTC AGTGCCAATG TGAGCATGCG CGTGTTGGTG 
GGTGACCGCA ACGACAACGC ACCACGCGTG CTGTATCCCA CACTTGAGCC CGACGGCTCT 
GCGCTCTTCG ACATGGTGCC GCGCGCTGCC GAGCCCGGAT ACCTGGTCAC CAAGGTGGTG 
GCTGTGGACG CAGACTCCGG ACACAATGCC TGGCTGTCTT ACCACGTGCT GCAGGCCAGC 
GATCCCGGGC TCTTCAGCCT GGGACTGCGC ACAGGCGAGG TGCGCACAGC GCGTGCCCTG 
GGAGACAGGG ACTCAGCGCG GCAGCGCCTT CTGGTCGCTG TGCGTGATGG TGGACAGCCA 
CCGCTCTCCG CTACAGCCAC GCTGCACCTC ATCTTCGCTG ACAGCCTGCA GGAGGTCCTG 
CCAGACCTCA GCGATGACCC TCTACCATCT GACCCTCAGA GTGAGCTTCA GTTTTACCTG 
GTGGTGGCCT TGGCCCTGAT CTCAGTGCTC TTCCTCCTGG CGGTGATTCT GGCTATTGCC 
CTGCGTCTGA GACACTCCTC CAGATCAGCT GCCTGGGGCT GCTTTCAGCC TGGCCTCAGC 
CTGAAGTCTC AACCTGTGGT TCCTCCCAAC TACAATGAGG GGACATTACC CTATTCCTAT 
AATCTCTGCG TTGCTTCACA TTCTACGACT ATGGCAGGGT TTAATTTTCA CCACGTGGCA 
CCAGAGGTTG CTCCCCCTCA GGATCTCCTG TGCGATGATC CTTCTGTGGT TGTATGTGGC 
AATAATGAAG ATCCCCAAGT CTCGGACGAC TCTGCTTTCC AGCAAGCCCC GCCCAACACT 
GACTGGCGTT TCTCTCAAGC CCAGAGACCC GGCACGAGCG GATCCCAAAA TGGTGATGAA 
ACTGGCACCT GGCCCAACAA CCAGTTTGAT ACAGAGATGC TGCAAGCCAT GATCTTGGCC 
TCTGCCAGCG AAGCTGCTGA TGGGAGCTCT ACCCTGGGAG GGGGCGCTGG CACCATGGGT 
CTGAGCGCTC GATACGGACC CCAGTTTACC CTGCAGCACG TGCCTGACTA CCGCCAGAAC 
GTGTACATCC CTGGCAGCAA TGCCACGCTG ACCAATGCCG CTGGCAAACG AGATGGCAAG 
GCTCCAGCAG GTGGTAATGG CAACAAGAAG AAATCGGGCA AGAAAGAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_291052.3
CDS	187..2943
Translation	MENQVLLHFLVPLFCGALSQPIHYSIPEELAKGSLVGSLAKDLGLSIQELPARKLRVSAEDYFSVSAESGDLLVSGRIDREKICGRKSECALEFESVTENPMNIFHVTVAIQDINDNAPHFFGKSIELEICESALAGAKFPLDSARDADVGSNSLKMYTVSPNPHFSLSTKESPDGSKYPELLLETPLDREHQSSHHLILTAMDGGDPPLSSTTQVWIKVTDANDNAPVFSQDTYKVSLRENIPPGTLVLQVTASDQDESIHGEITYAFLNAPASTNLVFNLNPNTGAITTNGTLDFEEKNRYTLGVEAKDGGVHTAHCNVQIEILDDNDNAPEVTFMSFSNQVPEDSVLGTVIALIKVRDKDSGQNGLVACHIQEELPFKLQPTSKNYYKLVIDKALNREQTPEYNVTITATDSGKPSLSSKTCVTVHITDINDNAPVFHQASYLVHVAENNPPGASIAQVSASDPDLGSNGLISYSIIASDLEPRALSSFVSVNQDSGVVFAQRAFDHEQLRSFQLTLQARDHGSPTLSANVSMRVLVGDRNDNAPRVLYPTLEPDGSALFDMVPRAAEPGYLVTKVVAVDADSGHNAWLSYHVLQASDPGLFSLGLRTGEVRTARALGDRDSARQRLLVAVRDGGQPPLSATATLHLIFADSLQEVLPDLSDDPLPSDPQSELQFYLVVALALISVLFLLAVILAIALRLRHSSRSAAWGCFQPGLSLKSQPVVPPNYNEGTLPYSYNLCVASHSTTMAGFNFHHVAPEVAPPQDLLCDDPSVVVCGNNEDPQVSDDSAFQQAPPNTDWRFSQAQRPGTSGSQNGDETGTWPNNQFDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQFTLQHVPDYRQNVYIPGSNATLTNAAGKRDGKAPAGGNGNKKKSGKKEKK

Target ORF information:

RefSeq Version	NM_033574.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protocadherin gamma subfamily B, 1 (Pcdhgb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033574.4

ORF Insert Sequence:

ATGGAAAATC AGGTATTGCT TCACTTCCTG GTGCCTTTGT TCTGCGGCGC CCTCTCCCAG 
CCCATCCACT ACTCCATTCC GGAGGAGCTA GCCAAGGGCT CTCTAGTGGG GAGTCTCGCC 
AAGGATCTGG GACTCAGTAT CCAAGAATTG CCAGCTCGAA AACTGAGAGT TAGTGCAGAG 
GATTATTTCA GCGTTAGTGC AGAGAGTGGG GATTTGTTAG TGAGCGGCAG GATAGACAGA 
GAAAAGATTT GTGGGAGGAA ATCTGAGTGT GCACTGGAAT TTGAATCTGT TACTGAAAAC 
CCGATGAACA TTTTCCACGT AACGGTTGCC ATCCAAGACA TTAACGATAA TGCACCACAT 
TTCTTTGGGA AAAGCATTGA ATTGGAGATC TGTGAGTCAG CCTTAGCAGG GGCAAAATTC 
CCTCTGGACT CTGCGCGAGA TGCAGATGTG GGAAGCAACT CCCTGAAGAT GTACACTGTC 
AGCCCCAATC CACACTTCTC CTTGTCAACG AAGGAAAGTC CTGATGGAAG TAAATACCCA 
GAACTGCTGC TGGAAACCCC TCTGGACAGG GAGCATCAGA GCTCCCATCA CTTAATACTG 
ACCGCCATGG ATGGTGGAGA CCCGCCCCTA AGCAGCACCA CCCAGGTCTG GATCAAAGTC 
ACCGACGCCA ATGACAATGC TCCAGTGTTC AGCCAGGACA CTTACAAAGT TAGCCTTCGT 
GAAAACATAC CCCCAGGAAC GTTAGTGCTG CAAGTGACAG CTTCTGATCA GGATGAGAGT 
ATTCACGGAG AGATCACCTA TGCCTTCCTT AATGCCCCAG CAAGTACTAA CCTTGTCTTC 
AATCTCAATC CAAATACTGG AGCTATCACA ACTAATGGTA CATTGGATTT TGAAGAAAAG 
AATAGATACA CATTGGGTGT AGAAGCCAAG GACGGAGGGG TGCACACGGC TCACTGTAAT 
GTTCAGATCG AAATTCTTGA TGACAACGAC AATGCCCCAG AGGTGACATT CATGTCCTTT 
TCTAACCAAG TTCCAGAGGA TTCAGTCCTT GGAACAGTAA TCGCCCTCAT TAAAGTTCGA 
GACAAAGATT CTGGACAAAA TGGTTTGGTG GCATGCCATA TTCAGGAAGA ACTTCCCTTC 
AAATTACAAC CCACCTCCAA GAATTATTAC AAGTTAGTGA TTGACAAGGC CCTAAACCGA 
GAACAGACCC CAGAGTACAA CGTCACTATC ACAGCTACTG ACAGTGGCAA GCCCTCGCTT 
TCCTCCAAGA CATGTGTTAC TGTACACATC ACAGATATCA ACGACAATGC ACCGGTTTTT 
CACCAGGCCT CCTACTTGGT CCACGTGGCT GAGAACAACC CACCTGGAGC CTCTATTGCT 
CAAGTCAGCG CCTCAGATCC GGATTTGGGA TCCAATGGCC TCATTTCCTA CTCCATCATA 
GCCAGCGACC TAGAGCCAAG GGCACTATCG TCCTTCGTGT CCGTGAACCA GGACAGTGGA 
GTGGTGTTCG CGCAGCGCGC CTTCGACCAC GAGCAGCTGC GTTCCTTCCA GCTGACACTG 
CAGGCGCGGG ACCACGGATC GCCCACACTC AGTGCCAATG TGAGCATGCG CGTGTTGGTG 
GGTGACCGCA ACGACAACGC ACCACGCGTG CTGTATCCCA CACTTGAGCC CGACGGCTCT 
GCGCTCTTCG ACATGGTGCC GCGCGCTGCC GAGCCCGGAT ACCTGGTCAC CAAGGTGGTG 
GCTGTGGACG CAGACTCCGG ACACAATGCC TGGCTGTCTT ACCACGTGCT GCAGGCCAGC 
GATCCCGGGC TCTTCAGCCT GGGACTGCGC ACAGGCGAGG TGCGCACAGC GCGTGCCCTG 
GGAGACAGGG ACTCAGCGCG GCAGCGCCTT CTGGTCGCTG TGCGTGATGG TGGACAGCCA 
CCGCTCTCCG CTACAGCCAC GCTGCACCTC ATCTTCGCTG ACAGCCTGCA GGAGGTCCTG 
CCAGACCTCA GCGATGACCC TCTACCATCT GACCCTCAGA GTGAGCTTCA GTTTTACCTG 
GTGGTGGCCT TGGCCCTGAT CTCAGTGCTC TTCCTCCTGG CGGTGATTCT GGCTATTGCC 
CTGCGTCTGA GACACTCCTC CAGATCAGCT GCCTGGGGCT GCTTTCAGCC TGGCCTCAGC 
CTGAAGTCTC AACCTGTGGT TCCTCCCAAC TACAATGAGG GGACATTACC CTATTCCTAT 
AATCTCTGCG TTGCTTCACA TTCTACGACT ATGGCAGGGT TTAATTTTCA CCACGTGGCA 
CCAGAGGTTG CTCCCCCTCA GGATCTCCTG TGCGATGATC CTTCTGTGGT TGTATGTGGC 
AATAATGAAG ATCCCCAAGT CTCGGACGAC TCTGCTTTCC AGCAAGCCCC GCCCAACACT 
GACTGGCGTT TCTCTCAAGC CCAGAGACCC GGCACGAGCG GATCCCAAAA TGGTGATGAA 
ACTGGCACCT GGCCCAACAA CCAGTTTGAT ACAGAGATGC TGCAAGCCAT GATCTTGGCC 
TCTGCCAGCG AAGCTGCTGA TGGGAGCTCT ACCCTGGGAG GGGGCGCTGG CACCATGGGT 
CTGAGCGCTC GATACGGACC CCAGTTTACC CTGCAGCACG TGCCTGACTA CCGCCAGAAC 
GTGTACATCC CTGGCAGCAA TGCCACGCTG ACCAATGCCG CTGGCAAACG AGATGGCAAG 
GCTCCAGCAG GTGGTAATGG CAACAAGAAG AAATCGGGCA AGAAAGAGAA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Maturation of postnatally generated olfactory bulb granule cells depends on functional ?-protocadherin expression.
Scientific reports31514(2013)
Ledderose J,Dieter S,Schwarz MK

Functional significance of isoform diversification in the protocadherin gamma gene cluster.
Neuron75(3)402-9(2012 Aug)
Chen WV,Alvarez FJ,Lefebvre JL,Friedman B,Nwakeze C,Geiman E,Smith C,Thu CA,Tapia JC,Tasic B,Sanes JR,Maniatis T

Combinatorial homophilic interaction between gamma-protocadherin multimers greatly expands the molecular diversity of cell adhesion.
Proceedings of the National Academy of Sciences of the United States of America107(33)14893-8(2010 Aug)
Schreiner D,Weiner JA

A differential developmental pattern of spinal interneuron apoptosis during synaptogenesis: insights from genetic analyses of the protocadherin-gamma gene cluster.
Development (Cambridge, England)135(24)4153-64(2008 Dec)
Prasad T,Wang X,Gray PA,Weiner JA

The b1 isoform of protocadherin-gamma (Pcdhgamma) interacts with the microtubule-destabilizing protein SCG10.
FEBS letters578(1-2)175-9(2004 Dec)
Gayet O,Labella V,Henderson CE,Kallenbach S

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Pcdhgb1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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