Pi4k2a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pi4k2a
Entrez Gene ID	84095
Full Name	phosphatidylinositol 4-kinase type 2 alpha
Synonyms	Pi4k2
General protein information	Preferred Names phosphatidylinositol 4-kinase type 2 alpha Names phosphatidylinositol 4-kinase type 2-alpha phosphatidylinositol 4-kinase type II-alpha
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 C3\|19 35.74 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011247406.2	XP_011245708.1	phosphatidylinositol 4-kinase type 2-alpha isoform X2
NM_145501.2	NP_663476.1	phosphatidylinositol 4-kinase type 2-alpha
XM_011247406.3	XP_011245708.1	phosphatidylinositol 4-kinase type 2-alpha isoform X2
XM_011247405.2	XP_011245707.1	phosphatidylinositol 4-kinase type 2-alpha isoform X1
XM_011247405.3	XP_011245707.1	phosphatidylinositol 4-kinase type 2-alpha isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PI4K2A	XP_543953.2
Rattus norvegicus (Norway rat)	Pi4k2a	NP_446187.1
Caenorhabditis elegans (roundworm)	ZC8.6	NP_508849.3
Drosophila melanogaster (fruit fly)	Pi4KIIalpha	NP_996154.1
Xenopus tropicalis (tropical clawed frog)	pi4k2a	NP_001263612.1
Homo sapiens (human)	PI4K2A	NP_060895.1
Pan troglodytes (chimpanzee)	PI4K2A	XP_507965.2
Bos taurus (cattle)	PI4K2A	NP_001093786.1
Mus musculus (house mouse)	Pi4k2a	NP_663476.1
Gallus gallus (chicken)	PI4K2A	XP_423069.1
Danio rerio (zebrafish)	pi4k2a	NP_998523.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003121	XP_562889.4

Gene Ontology
Bibliography

Related articles in PubMed

Type II PI4-kinases control Weibel-Palade body biogenesis and von Willebrand factor structure in human endothelial cells.
Lopes da Silva M, O'Connor MN, Kriston-Vizi J, White IJ, Al-Shawi R, Simons JP, M?ssinger J, Haucke V, Cutler DF
Journal of cell science129(10)2096-105(2016 05)

PI4KII? phosphorylation by GSK3 directs vesicular trafficking to lysosomes.
Robinson JW, Leshchyns'ka I, Farghaian H, Hughes WE, Sytnyk V, Neely GG, Cole AR
The Biochemical journal464(1)145-56(2014 Nov)

Phosphatidylinositol 4-kinase II? function at endosomes is regulated by the ubiquitin ligase Itch.
M?ssinger J, Wieffer M, Krause E, Freund C, Gerth F, Krauss M, Haucke V
EMBO reports13(12)1087-94(2012 Dec)

Differences in health status affect susceptibility and mapping of genetic loci for atherosclerosis (fatty streak) in inbred mice.
Srivastava U, Paigen BJ, Korstanje R
Arteriosclerosis, thrombosis, and vascular biology32(10)2380-6(2012 Oct)

The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse.
Larimore J, Tornieri K, Ryder PV, Gokhale A, Zlatic SA, Craige B, Lee JD, Talbot K, Pare JF, Smith Y, Faundez V
Molecular biology of the cell22(24)4854-67(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Itch directly associates with and ubiquitinates PI4KIIalpha, and both proteins colocalize on endosomes containing Wnt-activated frizzled 4 (Fz4) receptor
Title: Phosphatidylinositol 4-kinase II? function at endosomes is regulated by the ubiquitin ligase Itch.

PI4KIIalpha is a novel regulator of tumor growth by its action on angiogenesis and HIF-1alpha regulation.
Title: PI4KIIalpha is a novel regulator of tumor growth by its action on angiogenesis and HIF-1alpha regulation.

AP-3 and BLOC-1 act, either in concert or sequentially, to specify sorting of PI4KIIalpha along the endocytic route.
Title: Hermansky-Pudlak syndrome protein complexes associate with phosphatidylinositol 4-kinase type II alpha in neuronal and non-neuronal cells.

The unique N terminus of PI4K type IIalpha is required for the targeting of the enzyme to cellugyrin-positive vesicles.
Title: Phosphatidylinositol 4-kinase type IIalpha is targeted specifically to cellugyrin-positive glucose transporter 4 vesicles.

PI4KIIalpha is present on adaptor protein (AP) -3 organelles where it regulates AP-3 function
Title: Phosphatidylinositol-4-kinase type II alpha is a component of adaptor protein-3-derived vesicles.

The following Pi4k2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pi4k2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu11693	XM_011247405.3 Latest version!	Mus musculus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu71881	XM_011247406.3 Latest version!	Mus musculus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu11693	NM_145501.2 Latest version!	Mus musculus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OMu71881	XM_011247406.2	Mus musculus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OMu11693	XM_011247405.2	Mus musculus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pi4k2a ( XM_011247405.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245707.1 Mus musculus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu11693
Clone ID Related Accession (Same CDS sequence)	NM_145501.2 , XM_011247405.2 , XM_011247405.3
Accession Version	XM_011247405.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-kinase type 2-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247405.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGA CGAGCCCGCT AGTGTCCCCC GAGCGGGCCC AACCCCCGGA GTACACCTTC 
CCGTCGGGCT CCGGAGCTCA CTTTCCGCAA GTACCGGGGG GCGCGGTCCG CGTGGCGGCG 
GCGGCCGGCT CCGGCCCGTC ACCGCCGTGC TCGCCCGGCC ACGACCGGGA GCGGCAGCCC 
CTGCTGGACC GGGCCCGGGG CGCGGCGGCG CAGGGCCAGA CCCACACGGT GGCGGTGCAG 
GCCCAGGCCC TGGCCGCCCA AGCGGCCGTG GCGGCGCACG CCGTTCAGAC CCACCGCGAG 
CGGAACGACT TCCCGGAGGA CCCCGAGTTC GAGGTGGTGG TGCGGCAGGC CGAGGTTGCC 
ATCGAGTGCA GCATCTATCC CGAGCGCATC TACCAGGGCT CCAGTGGAAG CTACTTCGTC 
AAGGACTCTC AGGGGAGAAT CGTTGCTGTC TTCAAACCCA AGAATGAAGA GCCATACGGG 
CACCTTAACC CTAAGTGGAC CAAGTGGCTG CAGAAGCTGT GCTGCCCCTG CTGCTTTGGC 
CGAGACTGCC TTGTTCTCAA CCAGGGCTAT CTCTCAGAGG CAGGGGCTAG CCTGGTGGAC 
CAAAAACTGG AACTCAACAT TGTACCACGT ACAAAGGTAG TATACCTGGC CAGTGAAACC 
TTCAACTACA GTGCCATTGA TCGAGTAAAG TCCAGGGGCA AGCGGCTTGC ACTAGAGAAA 
GTGCCAAAAG TTGGGCAGCG GTTTAACCGA ATCGGCCTGC CACCAAAGGT CGGGTCATTC 
CAGCTCTTCG TTGAAGGCTA CAAAGATGCA GACTATTGGC TGCGGCGTTT TGAAGCAGAA 
CCTCTCCCTG AGAACACGAA CCGACAGCTG CTATTGCAGT TTGAGCGGTT GGTGGTCCTG 
GACTACATCA TCCGCAACAC TGACCGAGGC AATGACAACT GGTTGATCAA ATATGACTGT 
CCGATGGATA ATTCTAGCTG TCGGGACACA GATTGGGTGA TGGTGAGGGA GCCTGTTATC 
AAGGTGGCTG CCATAGACAA CGGGCTAGCT TTCCCACTGA AGCATCCTGA CTCCTGGAGG 
GCATATCCTT TTTACTGGGC CTGGCTGCCT CAGGCGAAAG TCCCGTTCTC TCAGGAGATC 
AAAGATTTGA TTCTTCCAAA GATTTCAGAC CCTAACTTCA TCAAGGACTT GGAGGAGGAC 
CTATATGAAC TCTTCAAGAG AGATCCTGGC TTCGACAGGG GCCAGTTCCA TAAGCAGATT 
GCTGTCATGA GAGGCCAGAT CCTAAATTTG ACCCAGGCCC TGAAAGACAA TAAGAGCCCC 
CTGCACCTCG TCCAGATGCC ACCTGTGATT GTCGAGACGG CCCGCTCTCA CCAGCGGTCT 
GCAAGCGAAT CCTACACACA GAGCTTTCAG AGTCGGAAGC CCTTCTTTTC ATGGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245707.1
CDS	354..1793
Translation	MDETSPLVSPERAQPPEYTFPSGSGAHFPQVPGGAVRVAAAAGSGPSPPCSPGHDRERQPLLDRARGAAAQGQTHTVAVQAQALAAQAAVAAHAVQTHRERNDFPEDPEFEVVVRQAEVAIECSIYPERIYQGSSGSYFVKDSQGRIVAVFKPKNEEPYGHLNPKWTKWLQKLCCPCCFGRDCLVLNQGYLSEAGASLVDQKLELNIVPRTKVVYLASETFNYSAIDRVKSRGKRLALEKVPKVGQRFNRIGLPPKVGSFQLFVEGYKDADYWLRRFEAEPLPENTNRQLLLQFERLVVLDYIIRNTDRGNDNWLIKYDCPMDNSSCRDTDWVMVREPVIKVAAIDNGLAFPLKHPDSWRAYPFYWAWLPQAKVPFSQEIKDLILPKISDPNFIKDLEEDLYELFKRDPGFDRGQFHKQIAVMRGQILNLTQALKDNKSPLHLVQMPPVIVETARSHQRSASESYTQSFQSRKPFFSWW

Target ORF information:

RefSeq Version	XM_011247405.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247405.3

ORF Insert Sequence:

ATGGACGAGA CGAGCCCGCT AGTGTCCCCC GAGCGGGCCC AACCCCCGGA GTACACCTTC 
CCGTCGGGCT CCGGAGCTCA CTTTCCGCAA GTACCGGGGG GCGCGGTCCG CGTGGCGGCG 
GCGGCCGGCT CCGGCCCGTC ACCGCCGTGC TCGCCCGGCC ACGACCGGGA GCGGCAGCCC 
CTGCTGGACC GGGCCCGGGG CGCGGCGGCG CAGGGCCAGA CCCACACGGT GGCGGTGCAG 
GCCCAGGCCC TGGCCGCCCA AGCGGCCGTG GCGGCGCACG CCGTTCAGAC CCACCGCGAG 
CGGAACGACT TCCCGGAGGA CCCCGAGTTC GAGGTGGTGG TGCGGCAGGC CGAGGTTGCC 
ATCGAGTGCA GCATCTATCC CGAGCGCATC TACCAGGGCT CCAGTGGAAG CTACTTCGTC 
AAGGACTCTC AGGGGAGAAT CGTTGCTGTC TTCAAACCCA AGAATGAAGA GCCATACGGG 
CACCTTAACC CTAAGTGGAC CAAGTGGCTG CAGAAGCTGT GCTGCCCCTG CTGCTTTGGC 
CGAGACTGCC TTGTTCTCAA CCAGGGCTAT CTCTCAGAGG CAGGGGCTAG CCTGGTGGAC 
CAAAAACTGG AACTCAACAT TGTACCACGT ACAAAGGTAG TATACCTGGC CAGTGAAACC 
TTCAACTACA GTGCCATTGA TCGAGTAAAG TCCAGGGGCA AGCGGCTTGC ACTAGAGAAA 
GTGCCAAAAG TTGGGCAGCG GTTTAACCGA ATCGGCCTGC CACCAAAGGT CGGGTCATTC 
CAGCTCTTCG TTGAAGGCTA CAAAGATGCA GACTATTGGC TGCGGCGTTT TGAAGCAGAA 
CCTCTCCCTG AGAACACGAA CCGACAGCTG CTATTGCAGT TTGAGCGGTT GGTGGTCCTG 
GACTACATCA TCCGCAACAC TGACCGAGGC AATGACAACT GGTTGATCAA ATATGACTGT 
CCGATGGATA ATTCTAGCTG TCGGGACACA GATTGGGTGA TGGTGAGGGA GCCTGTTATC 
AAGGTGGCTG CCATAGACAA CGGGCTAGCT TTCCCACTGA AGCATCCTGA CTCCTGGAGG 
GCATATCCTT TTTACTGGGC CTGGCTGCCT CAGGCGAAAG TCCCGTTCTC TCAGGAGATC 
AAAGATTTGA TTCTTCCAAA GATTTCAGAC CCTAACTTCA TCAAGGACTT GGAGGAGGAC 
CTATATGAAC TCTTCAAGAG AGATCCTGGC TTCGACAGGG GCCAGTTCCA TAAGCAGATT 
GCTGTCATGA GAGGCCAGAT CCTAAATTTG ACCCAGGCCC TGAAAGACAA TAAGAGCCCC 
CTGCACCTCG TCCAGATGCC ACCTGTGATT GTCGAGACGG CCCGCTCTCA CCAGCGGTCT 
GCAAGCGAAT CCTACACACA GAGCTTTCAG AGTCGGAAGC CCTTCTTTTC ATGGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi4k2a ( XM_011247406.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245708.1 Mus musculus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu71881
Clone ID Related Accession (Same CDS sequence)	XM_011247406.2 , XM_011247406.3
Accession Version	XM_011247406.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-kinase type 2-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247406.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGA CGAGCCCGCT AGTGTCCCCC GAGCGGGCCC AACCCCCGGA GTACACCTTC 
CCGTCGGGCT CCGGAGCTCA CTTTCCGCAA GTACCGGGGG GCGCGGTCCG CGTGGCGGCG 
GCGGCCGGCT CCGGCCCGTC ACCGCCGTGC TCGCCCGGCC ACGACCGGGA GCGGCAGCCC 
CTGCTGGACC GGGCCCGGGG CGCGGCGGCG CAGGGCCAGA CCCACACGGT GGCGGTGCAG 
GCCCAGGCCC TGGCCGCCCA AGCGGCCGTG GCGGCGCACG CCGTTCAGAC CCACCGCGAG 
CGGAACGACT TCCCGGAGGA CCCCGAGTTC GAGGTGGTGG TGCGGCAGGC CGAGGTTGCC 
ATCGAGTGCA GCATCTATCC CGAGCGCATC TACCAGGGCT CCAGTGGAAG CTACTTCGTC 
AAGGACTCTC AGGGGAGAAT CGTTGCTGTC TTCAAACCCA AGAATGAAGA GCCATACGGG 
CACCTTAACC CTAAGTGGAC CAAGTGGCTG CAGAAGCTGT GCTGCCCCTG CTGCTTTGGC 
CGAGACTGCC TTGTTCTCAA CCAGGGCTAT CTCTCAGAGG CAGGGGCTAG CCTGGTGGAC 
CAAAAACTGG AACTCAACAT TGTACCACGT ACAAAGGTAG TATACCTGGC CAGTGAAACC 
TTCAACTACA GTGCCATTGA TCGAGTAAAG TCCAGGGGCA AGCGGCTTGC ACTAGAGAAA 
GTGCCAAAAG TTGGGCAGCG GTTTAACCGA ATCGGCCTGC CACCAAAGGT CGGGTCATTC 
CAGCTCTTCG TTGAAGGCTA CAAAGATGCA GACTATTGGC TGCGGCGTTT TGAAGCAGAA 
CCTCTCCCTG AGAACACGAA CCGACAGCTG CTATTGCAGT TTGAGCGGTT GGTGGTCCTG 
GACTACATCA TCCGCAACAC TGACCGAGGC AATGACAACT GGTTGATCAA ATATGACTGT 
CCGATGGATA ATTCTAGCTG TCGGGACACA GATTGGGTGA TGGTGAGGGA GCCTGTTATC 
AAGGTGGCTG CCATAGACAA CGGGCTAGCT TTCCCACTGA AGCATCCTGA CTCCTGGAGG 
GCATATCCTT TTTACTGGGC CTGGCTGCCT CAGGCGAAAG TCCCGTTCTC TCAGGAGATC 
AAAGATTTGA TTCTTCCAAA GATTTCAGAC CCTAACTTCA TCAAGGACTT GGAGGAGGAC 
CTATATGAAC TCTTCAAGAT CCTAAATTTG ACCCAGGCCC TGAAAGACAA TAAGAGCCCC 
CTGCACCTCG TCCAGATGCC ACCTGTGATT GTCGAGACGG CCCGCTCTCA CCAGCGGTCT 
GCAAGCGAAT CCTACACACA GAGCTTTCAG AGTCGGAAGC CCTTCTTTTC ATGGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245708.1
CDS	355..1734
Translation	MDETSPLVSPERAQPPEYTFPSGSGAHFPQVPGGAVRVAAAAGSGPSPPCSPGHDRERQPLLDRARGAAAQGQTHTVAVQAQALAAQAAVAAHAVQTHRERNDFPEDPEFEVVVRQAEVAIECSIYPERIYQGSSGSYFVKDSQGRIVAVFKPKNEEPYGHLNPKWTKWLQKLCCPCCFGRDCLVLNQGYLSEAGASLVDQKLELNIVPRTKVVYLASETFNYSAIDRVKSRGKRLALEKVPKVGQRFNRIGLPPKVGSFQLFVEGYKDADYWLRRFEAEPLPENTNRQLLLQFERLVVLDYIIRNTDRGNDNWLIKYDCPMDNSSCRDTDWVMVREPVIKVAAIDNGLAFPLKHPDSWRAYPFYWAWLPQAKVPFSQEIKDLILPKISDPNFIKDLEEDLYELFKILNLTQALKDNKSPLHLVQMPPVIVETARSHQRSASESYTQSFQSRKPFFSWW

Target ORF information:

RefSeq Version	XM_011247406.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247406.3

ORF Insert Sequence:

ATGGACGAGA CGAGCCCGCT AGTGTCCCCC GAGCGGGCCC AACCCCCGGA GTACACCTTC 
CCGTCGGGCT CCGGAGCTCA CTTTCCGCAA GTACCGGGGG GCGCGGTCCG CGTGGCGGCG 
GCGGCCGGCT CCGGCCCGTC ACCGCCGTGC TCGCCCGGCC ACGACCGGGA GCGGCAGCCC 
CTGCTGGACC GGGCCCGGGG CGCGGCGGCG CAGGGCCAGA CCCACACGGT GGCGGTGCAG 
GCCCAGGCCC TGGCCGCCCA AGCGGCCGTG GCGGCGCACG CCGTTCAGAC CCACCGCGAG 
CGGAACGACT TCCCGGAGGA CCCCGAGTTC GAGGTGGTGG TGCGGCAGGC CGAGGTTGCC 
ATCGAGTGCA GCATCTATCC CGAGCGCATC TACCAGGGCT CCAGTGGAAG CTACTTCGTC 
AAGGACTCTC AGGGGAGAAT CGTTGCTGTC TTCAAACCCA AGAATGAAGA GCCATACGGG 
CACCTTAACC CTAAGTGGAC CAAGTGGCTG CAGAAGCTGT GCTGCCCCTG CTGCTTTGGC 
CGAGACTGCC TTGTTCTCAA CCAGGGCTAT CTCTCAGAGG CAGGGGCTAG CCTGGTGGAC 
CAAAAACTGG AACTCAACAT TGTACCACGT ACAAAGGTAG TATACCTGGC CAGTGAAACC 
TTCAACTACA GTGCCATTGA TCGAGTAAAG TCCAGGGGCA AGCGGCTTGC ACTAGAGAAA 
GTGCCAAAAG TTGGGCAGCG GTTTAACCGA ATCGGCCTGC CACCAAAGGT CGGGTCATTC 
CAGCTCTTCG TTGAAGGCTA CAAAGATGCA GACTATTGGC TGCGGCGTTT TGAAGCAGAA 
CCTCTCCCTG AGAACACGAA CCGACAGCTG CTATTGCAGT TTGAGCGGTT GGTGGTCCTG 
GACTACATCA TCCGCAACAC TGACCGAGGC AATGACAACT GGTTGATCAA ATATGACTGT 
CCGATGGATA ATTCTAGCTG TCGGGACACA GATTGGGTGA TGGTGAGGGA GCCTGTTATC 
AAGGTGGCTG CCATAGACAA CGGGCTAGCT TTCCCACTGA AGCATCCTGA CTCCTGGAGG 
GCATATCCTT TTTACTGGGC CTGGCTGCCT CAGGCGAAAG TCCCGTTCTC TCAGGAGATC 
AAAGATTTGA TTCTTCCAAA GATTTCAGAC CCTAACTTCA TCAAGGACTT GGAGGAGGAC 
CTATATGAAC TCTTCAAGAT CCTAAATTTG ACCCAGGCCC TGAAAGACAA TAAGAGCCCC 
CTGCACCTCG TCCAGATGCC ACCTGTGATT GTCGAGACGG CCCGCTCTCA CCAGCGGTCT 
GCAAGCGAAT CCTACACACA GAGCTTTCAG AGTCGGAAGC CCTTCTTTTC ATGGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi4k2a ( NM_145501.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663476.1 Mus musculus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), mRNA.

Price & Availability↑

CloneID	OMu11693
Clone ID Related Accession (Same CDS sequence)	NM_145501.2 , XM_011247405.2 , XM_011247405.3
Accession Version	NM_145501.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-kinase type 2-alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY732177.1, AK030725.1 and AC133503.4. On Feb 1, 2006 this sequence version replaced NM_145501.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK030725.1, BC110363.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGACGAGA CGAGCCCGCT AGTGTCCCCC GAGCGGGCCC AACCCCCGGA GTACACCTTC 
CCGTCGGGCT CCGGAGCTCA CTTTCCGCAA GTACCGGGGG GCGCGGTCCG CGTGGCGGCG 
GCGGCCGGCT CCGGCCCGTC ACCGCCGTGC TCGCCCGGCC ACGACCGGGA GCGGCAGCCC 
CTGCTGGACC GGGCCCGGGG CGCGGCGGCG CAGGGCCAGA CCCACACGGT GGCGGTGCAG 
GCCCAGGCCC TGGCCGCCCA AGCGGCCGTG GCGGCGCACG CCGTTCAGAC CCACCGCGAG 
CGGAACGACT TCCCGGAGGA CCCCGAGTTC GAGGTGGTGG TGCGGCAGGC CGAGGTTGCC 
ATCGAGTGCA GCATCTATCC CGAGCGCATC TACCAGGGCT CCAGTGGAAG CTACTTCGTC 
AAGGACTCTC AGGGGAGAAT CGTTGCTGTC TTCAAACCCA AGAATGAAGA GCCATACGGG 
CACCTTAACC CTAAGTGGAC CAAGTGGCTG CAGAAGCTGT GCTGCCCCTG CTGCTTTGGC 
CGAGACTGCC TTGTTCTCAA CCAGGGCTAT CTCTCAGAGG CAGGGGCTAG CCTGGTGGAC 
CAAAAACTGG AACTCAACAT TGTACCACGT ACAAAGGTAG TATACCTGGC CAGTGAAACC 
TTCAACTACA GTGCCATTGA TCGAGTAAAG TCCAGGGGCA AGCGGCTTGC ACTAGAGAAA 
GTGCCAAAAG TTGGGCAGCG GTTTAACCGA ATCGGCCTGC CACCAAAGGT CGGGTCATTC 
CAGCTCTTCG TTGAAGGCTA CAAAGATGCA GACTATTGGC TGCGGCGTTT TGAAGCAGAA 
CCTCTCCCTG AGAACACGAA CCGACAGCTG CTATTGCAGT TTGAGCGGTT GGTGGTCCTG 
GACTACATCA TCCGCAACAC TGACCGAGGC AATGACAACT GGTTGATCAA ATATGACTGT 
CCGATGGATA ATTCTAGCTG TCGGGACACA GATTGGGTGA TGGTGAGGGA GCCTGTTATC 
AAGGTGGCTG CCATAGACAA CGGGCTAGCT TTCCCACTGA AGCATCCTGA CTCCTGGAGG 
GCATATCCTT TTTACTGGGC CTGGCTGCCT CAGGCGAAAG TCCCGTTCTC TCAGGAGATC 
AAAGATTTGA TTCTTCCAAA GATTTCAGAC CCTAACTTCA TCAAGGACTT GGAGGAGGAC 
CTATATGAAC TCTTCAAGAG AGATCCTGGC TTCGACAGGG GCCAGTTCCA TAAGCAGATT 
GCTGTCATGA GAGGCCAGAT CCTAAATTTG ACCCAGGCCC TGAAAGACAA TAAGAGCCCC 
CTGCACCTCG TCCAGATGCC ACCTGTGATT GTCGAGACGG CCCGCTCTCA CCAGCGGTCT 
GCAAGCGAAT CCTACACACA GAGCTTTCAG AGTCGGAAGC CCTTCTTTTC ATGGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663476.1
CDS	68..1507
Translation	MDETSPLVSPERAQPPEYTFPSGSGAHFPQVPGGAVRVAAAAGSGPSPPCSPGHDRERQPLLDRARGAAAQGQTHTVAVQAQALAAQAAVAAHAVQTHRERNDFPEDPEFEVVVRQAEVAIECSIYPERIYQGSSGSYFVKDSQGRIVAVFKPKNEEPYGHLNPKWTKWLQKLCCPCCFGRDCLVLNQGYLSEAGASLVDQKLELNIVPRTKVVYLASETFNYSAIDRVKSRGKRLALEKVPKVGQRFNRIGLPPKVGSFQLFVEGYKDADYWLRRFEAEPLPENTNRQLLLQFERLVVLDYIIRNTDRGNDNWLIKYDCPMDNSSCRDTDWVMVREPVIKVAAIDNGLAFPLKHPDSWRAYPFYWAWLPQAKVPFSQEIKDLILPKISDPNFIKDLEEDLYELFKRDPGFDRGQFHKQIAVMRGQILNLTQALKDNKSPLHLVQMPPVIVETARSHQRSASESYTQSFQSRKPFFSWW

Target ORF information:

RefSeq Version	NM_145501.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145501.2

ORF Insert Sequence:

ATGGACGAGA CGAGCCCGCT AGTGTCCCCC GAGCGGGCCC AACCCCCGGA GTACACCTTC 
CCGTCGGGCT CCGGAGCTCA CTTTCCGCAA GTACCGGGGG GCGCGGTCCG CGTGGCGGCG 
GCGGCCGGCT CCGGCCCGTC ACCGCCGTGC TCGCCCGGCC ACGACCGGGA GCGGCAGCCC 
CTGCTGGACC GGGCCCGGGG CGCGGCGGCG CAGGGCCAGA CCCACACGGT GGCGGTGCAG 
GCCCAGGCCC TGGCCGCCCA AGCGGCCGTG GCGGCGCACG CCGTTCAGAC CCACCGCGAG 
CGGAACGACT TCCCGGAGGA CCCCGAGTTC GAGGTGGTGG TGCGGCAGGC CGAGGTTGCC 
ATCGAGTGCA GCATCTATCC CGAGCGCATC TACCAGGGCT CCAGTGGAAG CTACTTCGTC 
AAGGACTCTC AGGGGAGAAT CGTTGCTGTC TTCAAACCCA AGAATGAAGA GCCATACGGG 
CACCTTAACC CTAAGTGGAC CAAGTGGCTG CAGAAGCTGT GCTGCCCCTG CTGCTTTGGC 
CGAGACTGCC TTGTTCTCAA CCAGGGCTAT CTCTCAGAGG CAGGGGCTAG CCTGGTGGAC 
CAAAAACTGG AACTCAACAT TGTACCACGT ACAAAGGTAG TATACCTGGC CAGTGAAACC 
TTCAACTACA GTGCCATTGA TCGAGTAAAG TCCAGGGGCA AGCGGCTTGC ACTAGAGAAA 
GTGCCAAAAG TTGGGCAGCG GTTTAACCGA ATCGGCCTGC CACCAAAGGT CGGGTCATTC 
CAGCTCTTCG TTGAAGGCTA CAAAGATGCA GACTATTGGC TGCGGCGTTT TGAAGCAGAA 
CCTCTCCCTG AGAACACGAA CCGACAGCTG CTATTGCAGT TTGAGCGGTT GGTGGTCCTG 
GACTACATCA TCCGCAACAC TGACCGAGGC AATGACAACT GGTTGATCAA ATATGACTGT 
CCGATGGATA ATTCTAGCTG TCGGGACACA GATTGGGTGA TGGTGAGGGA GCCTGTTATC 
AAGGTGGCTG CCATAGACAA CGGGCTAGCT TTCCCACTGA AGCATCCTGA CTCCTGGAGG 
GCATATCCTT TTTACTGGGC CTGGCTGCCT CAGGCGAAAG TCCCGTTCTC TCAGGAGATC 
AAAGATTTGA TTCTTCCAAA GATTTCAGAC CCTAACTTCA TCAAGGACTT GGAGGAGGAC 
CTATATGAAC TCTTCAAGAG AGATCCTGGC TTCGACAGGG GCCAGTTCCA TAAGCAGATT 
GCTGTCATGA GAGGCCAGAT CCTAAATTTG ACCCAGGCCC TGAAAGACAA TAAGAGCCCC 
CTGCACCTCG TCCAGATGCC ACCTGTGATT GTCGAGACGG CCCGCTCTCA CCAGCGGTCT 
GCAAGCGAAT CCTACACACA GAGCTTTCAG AGTCGGAAGC CCTTCTTTTC ATGGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi4k2a ( XM_011247405.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245707.1 Mus musculus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), transcript variant X2, mRNA.

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CloneID	OMu11693
Clone ID Related Accession (Same CDS sequence)	NM_145501.2 , XM_011247405.2 , XM_011247405.3
Accession Version	XM_011247405.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-kinase type 2-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247405.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGA CGAGCCCGCT AGTGTCCCCC GAGCGGGCCC AACCCCCGGA GTACACCTTC 
CCGTCGGGCT CCGGAGCTCA CTTTCCGCAA GTACCGGGGG GCGCGGTCCG CGTGGCGGCG 
GCGGCCGGCT CCGGCCCGTC ACCGCCGTGC TCGCCCGGCC ACGACCGGGA GCGGCAGCCC 
CTGCTGGACC GGGCCCGGGG CGCGGCGGCG CAGGGCCAGA CCCACACGGT GGCGGTGCAG 
GCCCAGGCCC TGGCCGCCCA AGCGGCCGTG GCGGCGCACG CCGTTCAGAC CCACCGCGAG 
CGGAACGACT TCCCGGAGGA CCCCGAGTTC GAGGTGGTGG TGCGGCAGGC CGAGGTTGCC 
ATCGAGTGCA GCATCTATCC CGAGCGCATC TACCAGGGCT CCAGTGGAAG CTACTTCGTC 
AAGGACTCTC AGGGGAGAAT CGTTGCTGTC TTCAAACCCA AGAATGAAGA GCCATACGGG 
CACCTTAACC CTAAGTGGAC CAAGTGGCTG CAGAAGCTGT GCTGCCCCTG CTGCTTTGGC 
CGAGACTGCC TTGTTCTCAA CCAGGGCTAT CTCTCAGAGG CAGGGGCTAG CCTGGTGGAC 
CAAAAACTGG AACTCAACAT TGTACCACGT ACAAAGGTAG TATACCTGGC CAGTGAAACC 
TTCAACTACA GTGCCATTGA TCGAGTAAAG TCCAGGGGCA AGCGGCTTGC ACTAGAGAAA 
GTGCCAAAAG TTGGGCAGCG GTTTAACCGA ATCGGCCTGC CACCAAAGGT CGGGTCATTC 
CAGCTCTTCG TTGAAGGCTA CAAAGATGCA GACTATTGGC TGCGGCGTTT TGAAGCAGAA 
CCTCTCCCTG AGAACACGAA CCGACAGCTG CTATTGCAGT TTGAGCGGTT GGTGGTCCTG 
GACTACATCA TCCGCAACAC TGACCGAGGC AATGACAACT GGTTGATCAA ATATGACTGT 
CCGATGGATA ATTCTAGCTG TCGGGACACA GATTGGGTGA TGGTGAGGGA GCCTGTTATC 
AAGGTGGCTG CCATAGACAA CGGGCTAGCT TTCCCACTGA AGCATCCTGA CTCCTGGAGG 
GCATATCCTT TTTACTGGGC CTGGCTGCCT CAGGCGAAAG TCCCGTTCTC TCAGGAGATC 
AAAGATTTGA TTCTTCCAAA GATTTCAGAC CCTAACTTCA TCAAGGACTT GGAGGAGGAC 
CTATATGAAC TCTTCAAGAG AGATCCTGGC TTCGACAGGG GCCAGTTCCA TAAGCAGATT 
GCTGTCATGA GAGGCCAGAT CCTAAATTTG ACCCAGGCCC TGAAAGACAA TAAGAGCCCC 
CTGCACCTCG TCCAGATGCC ACCTGTGATT GTCGAGACGG CCCGCTCTCA CCAGCGGTCT 
GCAAGCGAAT CCTACACACA GAGCTTTCAG AGTCGGAAGC CCTTCTTTTC ATGGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245707.1
CDS	357..1796
Translation	MDETSPLVSPERAQPPEYTFPSGSGAHFPQVPGGAVRVAAAAGSGPSPPCSPGHDRERQPLLDRARGAAAQGQTHTVAVQAQALAAQAAVAAHAVQTHRERNDFPEDPEFEVVVRQAEVAIECSIYPERIYQGSSGSYFVKDSQGRIVAVFKPKNEEPYGHLNPKWTKWLQKLCCPCCFGRDCLVLNQGYLSEAGASLVDQKLELNIVPRTKVVYLASETFNYSAIDRVKSRGKRLALEKVPKVGQRFNRIGLPPKVGSFQLFVEGYKDADYWLRRFEAEPLPENTNRQLLLQFERLVVLDYIIRNTDRGNDNWLIKYDCPMDNSSCRDTDWVMVREPVIKVAAIDNGLAFPLKHPDSWRAYPFYWAWLPQAKVPFSQEIKDLILPKISDPNFIKDLEEDLYELFKRDPGFDRGQFHKQIAVMRGQILNLTQALKDNKSPLHLVQMPPVIVETARSHQRSASESYTQSFQSRKPFFSWW

Target ORF information:

RefSeq Version	XM_011247405.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247405.2

ORF Insert Sequence:

ATGGACGAGA CGAGCCCGCT AGTGTCCCCC GAGCGGGCCC AACCCCCGGA GTACACCTTC 
CCGTCGGGCT CCGGAGCTCA CTTTCCGCAA GTACCGGGGG GCGCGGTCCG CGTGGCGGCG 
GCGGCCGGCT CCGGCCCGTC ACCGCCGTGC TCGCCCGGCC ACGACCGGGA GCGGCAGCCC 
CTGCTGGACC GGGCCCGGGG CGCGGCGGCG CAGGGCCAGA CCCACACGGT GGCGGTGCAG 
GCCCAGGCCC TGGCCGCCCA AGCGGCCGTG GCGGCGCACG CCGTTCAGAC CCACCGCGAG 
CGGAACGACT TCCCGGAGGA CCCCGAGTTC GAGGTGGTGG TGCGGCAGGC CGAGGTTGCC 
ATCGAGTGCA GCATCTATCC CGAGCGCATC TACCAGGGCT CCAGTGGAAG CTACTTCGTC 
AAGGACTCTC AGGGGAGAAT CGTTGCTGTC TTCAAACCCA AGAATGAAGA GCCATACGGG 
CACCTTAACC CTAAGTGGAC CAAGTGGCTG CAGAAGCTGT GCTGCCCCTG CTGCTTTGGC 
CGAGACTGCC TTGTTCTCAA CCAGGGCTAT CTCTCAGAGG CAGGGGCTAG CCTGGTGGAC 
CAAAAACTGG AACTCAACAT TGTACCACGT ACAAAGGTAG TATACCTGGC CAGTGAAACC 
TTCAACTACA GTGCCATTGA TCGAGTAAAG TCCAGGGGCA AGCGGCTTGC ACTAGAGAAA 
GTGCCAAAAG TTGGGCAGCG GTTTAACCGA ATCGGCCTGC CACCAAAGGT CGGGTCATTC 
CAGCTCTTCG TTGAAGGCTA CAAAGATGCA GACTATTGGC TGCGGCGTTT TGAAGCAGAA 
CCTCTCCCTG AGAACACGAA CCGACAGCTG CTATTGCAGT TTGAGCGGTT GGTGGTCCTG 
GACTACATCA TCCGCAACAC TGACCGAGGC AATGACAACT GGTTGATCAA ATATGACTGT 
CCGATGGATA ATTCTAGCTG TCGGGACACA GATTGGGTGA TGGTGAGGGA GCCTGTTATC 
AAGGTGGCTG CCATAGACAA CGGGCTAGCT TTCCCACTGA AGCATCCTGA CTCCTGGAGG 
GCATATCCTT TTTACTGGGC CTGGCTGCCT CAGGCGAAAG TCCCGTTCTC TCAGGAGATC 
AAAGATTTGA TTCTTCCAAA GATTTCAGAC CCTAACTTCA TCAAGGACTT GGAGGAGGAC 
CTATATGAAC TCTTCAAGAG AGATCCTGGC TTCGACAGGG GCCAGTTCCA TAAGCAGATT 
GCTGTCATGA GAGGCCAGAT CCTAAATTTG ACCCAGGCCC TGAAAGACAA TAAGAGCCCC 
CTGCACCTCG TCCAGATGCC ACCTGTGATT GTCGAGACGG CCCGCTCTCA CCAGCGGTCT 
GCAAGCGAAT CCTACACACA GAGCTTTCAG AGTCGGAAGC CCTTCTTTTC ATGGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi4k2a ( XM_011247406.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245708.1 Mus musculus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), transcript variant X3, mRNA.

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CloneID	OMu71881
Clone ID Related Accession (Same CDS sequence)	XM_011247406.2 , XM_011247406.3
Accession Version	XM_011247406.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-kinase type 2-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247406.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGA CGAGCCCGCT AGTGTCCCCC GAGCGGGCCC AACCCCCGGA GTACACCTTC 
CCGTCGGGCT CCGGAGCTCA CTTTCCGCAA GTACCGGGGG GCGCGGTCCG CGTGGCGGCG 
GCGGCCGGCT CCGGCCCGTC ACCGCCGTGC TCGCCCGGCC ACGACCGGGA GCGGCAGCCC 
CTGCTGGACC GGGCCCGGGG CGCGGCGGCG CAGGGCCAGA CCCACACGGT GGCGGTGCAG 
GCCCAGGCCC TGGCCGCCCA AGCGGCCGTG GCGGCGCACG CCGTTCAGAC CCACCGCGAG 
CGGAACGACT TCCCGGAGGA CCCCGAGTTC GAGGTGGTGG TGCGGCAGGC CGAGGTTGCC 
ATCGAGTGCA GCATCTATCC CGAGCGCATC TACCAGGGCT CCAGTGGAAG CTACTTCGTC 
AAGGACTCTC AGGGGAGAAT CGTTGCTGTC TTCAAACCCA AGAATGAAGA GCCATACGGG 
CACCTTAACC CTAAGTGGAC CAAGTGGCTG CAGAAGCTGT GCTGCCCCTG CTGCTTTGGC 
CGAGACTGCC TTGTTCTCAA CCAGGGCTAT CTCTCAGAGG CAGGGGCTAG CCTGGTGGAC 
CAAAAACTGG AACTCAACAT TGTACCACGT ACAAAGGTAG TATACCTGGC CAGTGAAACC 
TTCAACTACA GTGCCATTGA TCGAGTAAAG TCCAGGGGCA AGCGGCTTGC ACTAGAGAAA 
GTGCCAAAAG TTGGGCAGCG GTTTAACCGA ATCGGCCTGC CACCAAAGGT CGGGTCATTC 
CAGCTCTTCG TTGAAGGCTA CAAAGATGCA GACTATTGGC TGCGGCGTTT TGAAGCAGAA 
CCTCTCCCTG AGAACACGAA CCGACAGCTG CTATTGCAGT TTGAGCGGTT GGTGGTCCTG 
GACTACATCA TCCGCAACAC TGACCGAGGC AATGACAACT GGTTGATCAA ATATGACTGT 
CCGATGGATA ATTCTAGCTG TCGGGACACA GATTGGGTGA TGGTGAGGGA GCCTGTTATC 
AAGGTGGCTG CCATAGACAA CGGGCTAGCT TTCCCACTGA AGCATCCTGA CTCCTGGAGG 
GCATATCCTT TTTACTGGGC CTGGCTGCCT CAGGCGAAAG TCCCGTTCTC TCAGGAGATC 
AAAGATTTGA TTCTTCCAAA GATTTCAGAC CCTAACTTCA TCAAGGACTT GGAGGAGGAC 
CTATATGAAC TCTTCAAGAT CCTAAATTTG ACCCAGGCCC TGAAAGACAA TAAGAGCCCC 
CTGCACCTCG TCCAGATGCC ACCTGTGATT GTCGAGACGG CCCGCTCTCA CCAGCGGTCT 
GCAAGCGAAT CCTACACACA GAGCTTTCAG AGTCGGAAGC CCTTCTTTTC ATGGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245708.1
CDS	358..1737
Translation	MDETSPLVSPERAQPPEYTFPSGSGAHFPQVPGGAVRVAAAAGSGPSPPCSPGHDRERQPLLDRARGAAAQGQTHTVAVQAQALAAQAAVAAHAVQTHRERNDFPEDPEFEVVVRQAEVAIECSIYPERIYQGSSGSYFVKDSQGRIVAVFKPKNEEPYGHLNPKWTKWLQKLCCPCCFGRDCLVLNQGYLSEAGASLVDQKLELNIVPRTKVVYLASETFNYSAIDRVKSRGKRLALEKVPKVGQRFNRIGLPPKVGSFQLFVEGYKDADYWLRRFEAEPLPENTNRQLLLQFERLVVLDYIIRNTDRGNDNWLIKYDCPMDNSSCRDTDWVMVREPVIKVAAIDNGLAFPLKHPDSWRAYPFYWAWLPQAKVPFSQEIKDLILPKISDPNFIKDLEEDLYELFKILNLTQALKDNKSPLHLVQMPPVIVETARSHQRSASESYTQSFQSRKPFFSWW

Target ORF information:

RefSeq Version	XM_011247406.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247406.2

ORF Insert Sequence:

ATGGACGAGA CGAGCCCGCT AGTGTCCCCC GAGCGGGCCC AACCCCCGGA GTACACCTTC 
CCGTCGGGCT CCGGAGCTCA CTTTCCGCAA GTACCGGGGG GCGCGGTCCG CGTGGCGGCG 
GCGGCCGGCT CCGGCCCGTC ACCGCCGTGC TCGCCCGGCC ACGACCGGGA GCGGCAGCCC 
CTGCTGGACC GGGCCCGGGG CGCGGCGGCG CAGGGCCAGA CCCACACGGT GGCGGTGCAG 
GCCCAGGCCC TGGCCGCCCA AGCGGCCGTG GCGGCGCACG CCGTTCAGAC CCACCGCGAG 
CGGAACGACT TCCCGGAGGA CCCCGAGTTC GAGGTGGTGG TGCGGCAGGC CGAGGTTGCC 
ATCGAGTGCA GCATCTATCC CGAGCGCATC TACCAGGGCT CCAGTGGAAG CTACTTCGTC 
AAGGACTCTC AGGGGAGAAT CGTTGCTGTC TTCAAACCCA AGAATGAAGA GCCATACGGG 
CACCTTAACC CTAAGTGGAC CAAGTGGCTG CAGAAGCTGT GCTGCCCCTG CTGCTTTGGC 
CGAGACTGCC TTGTTCTCAA CCAGGGCTAT CTCTCAGAGG CAGGGGCTAG CCTGGTGGAC 
CAAAAACTGG AACTCAACAT TGTACCACGT ACAAAGGTAG TATACCTGGC CAGTGAAACC 
TTCAACTACA GTGCCATTGA TCGAGTAAAG TCCAGGGGCA AGCGGCTTGC ACTAGAGAAA 
GTGCCAAAAG TTGGGCAGCG GTTTAACCGA ATCGGCCTGC CACCAAAGGT CGGGTCATTC 
CAGCTCTTCG TTGAAGGCTA CAAAGATGCA GACTATTGGC TGCGGCGTTT TGAAGCAGAA 
CCTCTCCCTG AGAACACGAA CCGACAGCTG CTATTGCAGT TTGAGCGGTT GGTGGTCCTG 
GACTACATCA TCCGCAACAC TGACCGAGGC AATGACAACT GGTTGATCAA ATATGACTGT 
CCGATGGATA ATTCTAGCTG TCGGGACACA GATTGGGTGA TGGTGAGGGA GCCTGTTATC 
AAGGTGGCTG CCATAGACAA CGGGCTAGCT TTCCCACTGA AGCATCCTGA CTCCTGGAGG 
GCATATCCTT TTTACTGGGC CTGGCTGCCT CAGGCGAAAG TCCCGTTCTC TCAGGAGATC 
AAAGATTTGA TTCTTCCAAA GATTTCAGAC CCTAACTTCA TCAAGGACTT GGAGGAGGAC 
CTATATGAAC TCTTCAAGAT CCTAAATTTG ACCCAGGCCC TGAAAGACAA TAAGAGCCCC 
CTGCACCTCG TCCAGATGCC ACCTGTGATT GTCGAGACGG CCCGCTCTCA CCAGCGGTCT 
GCAAGCGAAT CCTACACACA GAGCTTTCAG AGTCGGAAGC CCTTCTTTTC ATGGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Type II PI4-kinases control Weibel-Palade body biogenesis and von Willebrand factor structure in human endothelial cells.
Journal of cell science129(10)2096-105(2016 05)
Lopes da Silva M,O'Connor MN,Kriston-Vizi J,White IJ,Al-Shawi R,Simons JP,M?ssinger J,Haucke V,Cutler DF

PI4KII? phosphorylation by GSK3 directs vesicular trafficking to lysosomes.
The Biochemical journal464(1)145-56(2014 Nov)
Robinson JW,Leshchyns'ka I,Farghaian H,Hughes WE,Sytnyk V,Neely GG,Cole AR

Phosphatidylinositol 4-kinase II? function at endosomes is regulated by the ubiquitin ligase Itch.
EMBO reports13(12)1087-94(2012 Dec)
M?ssinger J,Wieffer M,Krause E,Freund C,Gerth F,Krauss M,Haucke V

Differences in health status affect susceptibility and mapping of genetic loci for atherosclerosis (fatty streak) in inbred mice.
Arteriosclerosis, thrombosis, and vascular biology32(10)2380-6(2012 Oct)
Srivastava U,Paigen BJ,Korstanje R

The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse.
Molecular biology of the cell22(24)4854-67(2011 Dec)
Larimore J,Tornieri K,Ryder PV,Gokhale A,Zlatic SA,Craige B,Lee JD,Talbot K,Pare JF,Smith Y,Faundez V

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Pi4k2a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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