Mmp1b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mmp1b
Entrez Gene ID	83996
Full Name	matrix metallopeptidase 1b (interstitial collagenase)
Synonyms	MMP-1b,Mcol-B,Mcolb
General protein information	Preferred Names matrix metallopeptidase 1b (interstitial collagenase) Names interstitial collagenase B collagenase-like B matrix metalloproteinase 1b (interstitial collagenase)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A1
Summary	This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. [provided by RefSeq, Feb 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_032007.3	NP_114396.3	interstitial collagenase B preproprotein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mmp1b	NP_114396.3
Rattus norvegicus (Norway rat)	Mmp1b	XP_006242575.1
Rattus norvegicus (Norway rat)	LOC100910997	XP_006242548.1

Gene Ontology
Bibliography

Related articles in PubMed

Functional interactions between matrix metalloproteinases and glycosaminoglycans.
Tocchi A, Parks WC
The FEBS journal280(10)2332-41(2013 May)

The role of macrophages in the disappearance of Meckel's cartilage during mandibular development in mice.
Tsuzurahara F, Soeta S, Kawawa T, Baba K, Nakamura M
Acta histochemica113(2)194-200(2011 Feb)

Endocardial Brg1 represses ADAMTS1 to maintain the microenvironment for myocardial morphogenesis.
Stankunas K, Hang CT, Tsun ZY, Chen H, Lee NV, Wu JI, Shang C, Bayle JH, Shou W, Iruela-Arispe ML, Chang CP
Developmental cell14(2)298-311(2008 Feb)

Expression of extracellular matrix metalloproteinase inducer and matrix metalloproteinases during mouse embryonic development.
Chen L, Nakai M, Belton RJ Jr, Nowak RA
Reproduction (Cambridge, England)133(2)405-14(2007 Feb)

Dysregulation of TGF-beta1 receptor activation leads to abnormal lung development and emphysema-like phenotype in core fucose-deficient mice.
Wang X, Inoue S, Gu J, Miyoshi E, Noda K, Li W, Mizuno-Horikawa Y, Nakano M, Asahi M, Takahashi M, Uozumi N, Ihara S, Lee SH, Ikeda Y, Yamaguchi Y, Aze Y, Tomiyama Y, Fujii J, Suzuki K, Kondo A, Shapiro SD, Lopez-Otin C, Kuwaki T, Okabe M, Honke K, Taniguchi N
Proceedings of the National Academy of Sciences of the United States of America102(44)15791-6(2005 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mmp1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mmp1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08668	NM_032007.3 Latest version!	Mus musculus matrix metallopeptidase 1b (interstitial collagenase) (Mmp1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mmp1b ( NM_032007.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_114396.3 Mus musculus matrix metallopeptidase 1b (interstitial collagenase) (Mmp1b), mRNA.

Price & Availability↑

CloneID	OMu08668
Clone ID Related Accession (Same CDS sequence)	NM_032007.3
Accession Version	NM_032007.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	interstitial collagenase B preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC169512.1. On Sep 28, 2006 this sequence version replaced NM_032007.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AJ278461.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGTCTAGCC TTCCTTTGCT GTTGCTTCTC TGGGCTGCCA GCTCATACAG TTTCCCTGTG 
ATTCAGGATG GACTCCAGAA AAATGTGAAG ACAGTCTGGA AATACCTGGA AAACTACTAC 
AACTTGGGCA AAAACATGCA AGCTAAAAAC GTGAATGGCA AGGAGGTGAT GGCTGAAAAG 
CTGAGGCAAA TGCAGCAGTT ATTTGGGCTG AAAGTAACTG GAAATTCAGA TCCTGAAACC 
CTGAGAGCTA TGAAGAAGCC CAGGTGTGGG GTGCCTGATG TGGCCCCATA TGCCATTACT 
CACAACAATC CTCGTTGGAC CAAAACACAT CTGACTTACA GCATTTTAAA CTACACACCA 
TATTTGTCAA AAGCAGTTGT GGAAGATGCC ATCGCGAGAG CCTTTAGAGT CTGGAGTGAT 
GTGACACCAC TTACGTTCCA AAGAGTCTTT GAGGAGGAAG GCGATATTGT GCTCTCCTTC 
CACAGAGGAG ACCATGGTGA CCTCTACACA TTTGATGGAT CTAAATATCA CTTTGCTCAT 
GCTTTTCTGC CAGGCCTAGG TTTGGGGGGC AATGTTCATT ATGATCTTGA TCAGAAGTGG 
ACCGACAACA ATGAGGATTT CAACTTGTTC TATGTTACGG CTCATGAACT GGGTCACTCC 
CTTGGACTCT CCCATTCTAA TGATGAAGAG GCACTAATGT TCCCCAGCTA CACATGGAGC 
AATAAAGACT TTGTGCTAAA CCAGGATGAT ATTAATCGCA TCCAGGCCTT ATATGGACCT 
TCCCCAAATC CCATCCAGTT AACAGATGCA ACACTGGATC CATGTAATAG TGGTCTAACC 
TTTGATGCTA TAATTACATA TCGGGGGGAG GTGATATTCT TTAAAGACAG GTTCTACATT 
CGGGTAATTA GCTTCCTGCC AGAACCTTTG ATCGATGTGA TAGACCTTAT CTGGCCAAAT 
CTGCCAGGTA AATTTGATGC AGCTTATGAA GTTAGTGGGG TAGATGAACT CCGCTTTTTC 
AAAGGCAGCA AAGTATGGGC TGTTCAGGAA CAGAATGTAC TGGAAGGATT TCCCATGGAC 
ATCCAGAGTT TCTTTGGCTT CCCTAGCAAT GTGACAAACA TTGATGCTGC TGTTTGTGAA 
GAAGAGACTG GAAAAACATA CTTCTTTGTT GACCACATGT ACTGGAGATA TGATGAAAAT 
ACACGGTCCA TGGATCCAGG TTACCCCAGA TTAATAGCAG AAGACTTTCC TGGAATCGAC 
TATAAAGTTG ATGATGTTAT CCAGAAAGAG GATAATTTCT ATTTCTTTCA CCAATCAATT 
CAATACAGAT TTAACCTCAA AACAAGAAGA ATTGATGATT CCAGTGATAT TAATACTTGG 
TTCAACTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114396.3
CDS	35..1426
Translation	MSSLPLLLLLWAASSYSFPVIQDGLQKNVKTVWKYLENYYNLGKNMQAKNVNGKEVMAEKLRQMQQLFGLKVTGNSDPETLRAMKKPRCGVPDVAPYAITHNNPRWTKTHLTYSILNYTPYLSKAVVEDAIARAFRVWSDVTPLTFQRVFEEEGDIVLSFHRGDHGDLYTFDGSKYHFAHAFLPGLGLGGNVHYDLDQKWTDNNEDFNLFYVTAHELGHSLGLSHSNDEEALMFPSYTWSNKDFVLNQDDINRIQALYGPSPNPIQLTDATLDPCNSGLTFDAIITYRGEVIFFKDRFYIRVISFLPEPLIDVIDLIWPNLPGKFDAAYEVSGVDELRFFKGSKVWAVQEQNVLEGFPMDIQSFFGFPSNVTNIDAAVCEEETGKTYFFVDHMYWRYDENTRSMDPGYPRLIAEDFPGIDYKVDDVIQKEDNFYFFHQSIQYRFNLKTRRIDDSSDINTWFNC

Target ORF information:

RefSeq Version	NM_032007.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus matrix metallopeptidase 1b (interstitial collagenase) (Mmp1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032007.3

ORF Insert Sequence:

ATGTCTAGCC TTCCTTTGCT GTTGCTTCTC TGGGCTGCCA GCTCATACAG TTTCCCTGTG 
ATTCAGGATG GACTCCAGAA AAATGTGAAG ACAGTCTGGA AATACCTGGA AAACTACTAC 
AACTTGGGCA AAAACATGCA AGCTAAAAAC GTGAATGGCA AGGAGGTGAT GGCTGAAAAG 
CTGAGGCAAA TGCAGCAGTT ATTTGGGCTG AAAGTAACTG GAAATTCAGA TCCTGAAACC 
CTGAGAGCTA TGAAGAAGCC CAGGTGTGGG GTGCCTGATG TGGCCCCATA TGCCATTACT 
CACAACAATC CTCGTTGGAC CAAAACACAT CTGACTTACA GCATTTTAAA CTACACACCA 
TATTTGTCAA AAGCAGTTGT GGAAGATGCC ATCGCGAGAG CCTTTAGAGT CTGGAGTGAT 
GTGACACCAC TTACGTTCCA AAGAGTCTTT GAGGAGGAAG GCGATATTGT GCTCTCCTTC 
CACAGAGGAG ACCATGGTGA CCTCTACACA TTTGATGGAT CTAAATATCA CTTTGCTCAT 
GCTTTTCTGC CAGGCCTAGG TTTGGGGGGC AATGTTCATT ATGATCTTGA TCAGAAGTGG 
ACCGACAACA ATGAGGATTT CAACTTGTTC TATGTTACGG CTCATGAACT GGGTCACTCC 
CTTGGACTCT CCCATTCTAA TGATGAAGAG GCACTAATGT TCCCCAGCTA CACATGGAGC 
AATAAAGACT TTGTGCTAAA CCAGGATGAT ATTAATCGCA TCCAGGCCTT ATATGGACCT 
TCCCCAAATC CCATCCAGTT AACAGATGCA ACACTGGATC CATGTAATAG TGGTCTAACC 
TTTGATGCTA TAATTACATA TCGGGGGGAG GTGATATTCT TTAAAGACAG GTTCTACATT 
CGGGTAATTA GCTTCCTGCC AGAACCTTTG ATCGATGTGA TAGACCTTAT CTGGCCAAAT 
CTGCCAGGTA AATTTGATGC AGCTTATGAA GTTAGTGGGG TAGATGAACT CCGCTTTTTC 
AAAGGCAGCA AAGTATGGGC TGTTCAGGAA CAGAATGTAC TGGAAGGATT TCCCATGGAC 
ATCCAGAGTT TCTTTGGCTT CCCTAGCAAT GTGACAAACA TTGATGCTGC TGTTTGTGAA 
GAAGAGACTG GAAAAACATA CTTCTTTGTT GACCACATGT ACTGGAGATA TGATGAAAAT 
ACACGGTCCA TGGATCCAGG TTACCCCAGA TTAATAGCAG AAGACTTTCC TGGAATCGAC 
TATAAAGTTG ATGATGTTAT CCAGAAAGAG GATAATTTCT ATTTCTTTCA CCAATCAATT 
CAATACAGAT TTAACCTCAA AACAAGAAGA ATTGATGATT CCAGTGATAT TAATACTTGG 
TTCAACTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional interactions between matrix metalloproteinases and glycosaminoglycans.
The FEBS journal280(10)2332-41(2013 May)
Tocchi A,Parks WC

The role of macrophages in the disappearance of Meckel's cartilage during mandibular development in mice.
Acta histochemica113(2)194-200(2011 Feb)
Tsuzurahara F,Soeta S,Kawawa T,Baba K,Nakamura M

Endocardial Brg1 represses ADAMTS1 to maintain the microenvironment for myocardial morphogenesis.
Developmental cell14(2)298-311(2008 Feb)
Stankunas K,Hang CT,Tsun ZY,Chen H,Lee NV,Wu JI,Shang C,Bayle JH,Shou W,Iruela-Arispe ML,Chang CP

Expression of extracellular matrix metalloproteinase inducer and matrix metalloproteinases during mouse embryonic development.
Reproduction (Cambridge, England)133(2)405-14(2007 Feb)
Chen L,Nakai M,Belton RJ Jr,Nowak RA

Dysregulation of TGF-beta1 receptor activation leads to abnormal lung development and emphysema-like phenotype in core fucose-deficient mice.
Proceedings of the National Academy of Sciences of the United States of America102(44)15791-6(2005 Nov)
Wang X,Inoue S,Gu J,Miyoshi E,Noda K,Li W,Mizuno-Horikawa Y,Nakano M,Asahi M,Takahashi M,Uozumi N,Ihara S,Lee SH,Ikeda Y,Yamaguchi Y,Aze Y,Tomiyama Y,Fujii J,Suzuki K,Kondo A,Shapiro SD,Lopez-Otin C,Kuwaki T,Okabe M,Honke K,Taniguchi N

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Mmp1b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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