Sf3b1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sf3b1
Entrez Gene ID	81898
Full Name	splicing factor 3b, subunit 1
Synonyms	155kDa,2810001M05Rik,AA409119,Prp10,SAP155,SF3b155,TA-8,Targ4
General protein information	Preferred Names splicing factor 3b, subunit 1 Names splicing factor 3B subunit 1 SAP 155 pre-mRNA-splicing factor SF3b 155 kDa subunit spliceosome-associated protein 155 splicing factor 3b, subunit 1, 155 kDa transforming growth factor alpha regulated gene 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 C1.2\|1 27.98 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_030243736.1	XP_030099596.1	splicing factor 3B subunit 1 isoform X1
XM_017312972.1	XP_017168461.1	splicing factor 3B subunit 1 isoform X1
XM_017312973.1	XP_017168462.1	splicing factor 3B subunit 1 isoform X1
NM_031179.2	NP_112456.2	splicing factor 3B subunit 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SF3B1	XP_545578.2
Mus musculus (house mouse)	Sf3b1	NP_112456.2
Drosophila melanogaster (fruit fly)	CG2807	NP_001259837.1
Arabidopsis thaliana (thale cress)	AT5G64270	NP_201232.1
Pan troglodytes (chimpanzee)	SF3B1	XP_516006.3
	SF3B1	XP_001086317.2
Rattus norvegicus (Norway rat)	Sf3b1	NP_445878.1
Gallus gallus (chicken)	SF3B1	XP_421912.3
Schizosaccharomyces pombe (fission yeast)	prp10	NP_594538.2
Oryza sativa (rice)	Os02g0147300	NP_001045883.1
Homo sapiens (human)	SF3B1	NP_036565.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000178	XP_310958.5
Magnaporthe oryzae (rice blast fungus)	MGG_00356	XP_003718725.1
Bos taurus (cattle)	SF3B1	NP_001179923.1
Danio rerio (zebrafish)	sf3b1	XP_005167843.1
Caenorhabditis elegans (roundworm)	T08A11.2	NP_497853.1
Neurospora crassa	NCU03042	XP_964047.1
Xenopus tropicalis (tropical clawed frog)	sf3b1	XP_002931569.2

Gene Ontology
Bibliography

Related articles in PubMed

Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.
Cui W, Dai X, Marcho C, Han Z, Zhang K, Tremblay KD, Mager J
Scientific reports637396(2016 Nov)

Physiologic Expression of Sf3b1(K700E) Causes Impaired Erythropoiesis, Aberrant Splicing, and Sensitivity to Therapeutic Spliceosome Modulation.
Obeng EA, Chappell RJ, Seiler M, Chen MC, Campagna DR, Schmidt PJ, Schneider RK, Lord AM, Wang L, Gambe RG, McConkey ME, Ali AM, Raza A, Yu L, Buonamici S, Smith PG, Mullally A, Wu CJ, Fleming MD, Ebert BL
Cancer cell30(3)404-417(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our findings demonstrate that, despite significant differences in affected transcripts, there is overlap in the phenotypes associated with SF3B1-K700E between human and mouse.
Title: Hemopoietic-specific Sf3b1-K700E knock-in mice display the splicing defect seen in human MDS but develop anemia without ring sideroblasts.

Sf3b1(K700E) mice develop macrocytic anemia due to a terminal erythroid maturation defect, erythroid dysplasia, and long-term hematopoietic stem cell (LT-HSC) expansion.
Title: Physiologic Expression of Sf3b1(K700E) Causes Impaired Erythropoiesis, Aberrant Splicing, and Sensitivity to Therapeutic Spliceosome Modulation.

myocardial hypoxia actuates fructose metabolism in human and mouse models of pathological cardiac hypertrophy through hypoxia-inducible factor 1alpha (HIF1alpha) activation of SF3B1 and SF3B1-mediated splice switching of KHK-A to KHK-C
Title: HIF-driven SF3B1 induces KHK-C to enforce fructolysis and heart disease.

SF3B1 plays an important role in the regulation of hematopoietic stem cells, whereas SF3B1 haploinsufficiency itself is not associated with the myelodysplastic syndrome phenotype with ring sideroblasts.
Title: Haploinsufficiency of Sf3b1 leads to compromised stem cell function but not to myelodysplasia.

The level of Sf3b1 expression is critical for the proliferative capacity of hematopoietic stem cells. Depletion of Sf3b1 impairs proliferative capacity of hematopoietic stem cells but is not sufficient to induce myelodysplasia.
Title: Depletion of Sf3b1 impairs proliferative capacity of hematopoietic stem cells but is not sufficient to induce myelodysplasia.

The following Sf3b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sf3b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu27668	XM_030243736.1 Latest version!	Mus musculus splicing factor 3b, subunit 1 (Sf3b1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu20984	NM_031179.2 Latest version!	Mus musculus splicing factor 3b, subunit 1 (Sf3b1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu27668	XM_017312972.1 Latest version!	Mus musculus splicing factor 3b, subunit 1 (Sf3b1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu27668	XM_017312973.1 Latest version!	Mus musculus splicing factor 3b, subunit 1 (Sf3b1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sf3b1 ( XM_030243736.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099596.1 Mus musculus splicing factor 3b, subunit 1 (Sf3b1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu27668
Clone ID Related Accession (Same CDS sequence)	XM_030243736.1 , XM_017312972.1 , XM_017312973.1
Accession Version	XM_030243736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	splicing factor 3B subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCTA GAACCTACAT GGATGTTATG CGAGAACAAC ATTTGACTAA GGAAGAGAGA 
GAAATTAGGC AACAACTAGC AGAAAAAGCT AAAGCTGGAG AACTAAAAGT CGTCAATGGA 
GCAGCAGCAT CCCAGCCTCC CTCAAAACGA AAACGGCGCT GGGATCAGAC CGCTGACCAG 
ACTCCTGGTG CCACTCCCAA AAAGCTATCG AGTTGGGATC AGGCAGAGAC CCCTGGGCAT 
ACCCCATCTT TAAGATGGGA TGAGACACCG GGTCGTGCAA AAGGAAGTGA AACACCTGGC 
GCAACCCCAG GCTCAAAAAT ATGGGACCCT ACACCGAGTC ATACACCTGC GGGAGCTGCT 
ACTCCTGGGC GAGGCGACAC ACCAGGCCAT GCAACCCCGG GCCATGGTGG TGCAACTTCC 
AGTGCTCGTA AAAACAGATG GGATGAGACC CCCAAAACAG AAAGAGATAC TCCTGGGCAT 
GGAAGTGGAT GGGCTGAGAC TCCTCGAACA GATCGAGGTG GAGACTCTAT TGGTGAAACA 
CCAACTCCTG GAGCAAGTAA AAGAAAGTCT CGTTGGGATG AAACGCCGGC GAGTCAAATG 
GGTGGAAGCA CTCCTGTTCT GACTCCAGGA AAAACACCAA TTGGCACACC AGCCATGAAC 
ATGGCTACCC CGACTCCAGG TCACATAATG AGCATGACAC CTGAACAGCT TCAGGCGTGG 
CGGTGGGAGA GAGAAATTGA TGAACGAAAC CGCCCACTTT CTGATGAAGA ATTAGATGCT 
ATGTTCCCAG AAGGATATAA GGTACTTCCC CCTCCAGCTG GTTATGTTCC TATTCGAACT 
CCAGCTCGAA AGCTGACAGC AACTCCTACA CCTTTGGGTG GTATGACTGG TTTCCACATG 
CAAACTGAAG ACAGAACTAT GAAAAGTGTC AATGACCAGC CATCTGGGAA TCTTCCATTT 
TTAAAACCTG ATGACATTCA GTACTTTGAT AAACTATTGG TTGATGTAGA TGAATCAACA 
CTTAGTCCAG AAGAACAAAA AGAAAGAAAA ATAATGAAGT TGCTTTTAAA AATTAAGAAT 
GGTACACCTC CAATGAGAAA GGCTGCCTTA CGTCAGATTA CTGATAAAGC TCGAGAATTT 
GGAGCTGGAC CACTGTTTAA CCAGATTCTT CCTCTGTTGA TGTCCCCTAC ACTTGAGGAT 
CAAGAGCGAC ATTTACTTGT GAAAGTTATT GATAGAATAT TGTATAAACT TGATGACTTG 
GTGCGGCCGT ATGTGCACAA GATTCTTGTG GTTATCGAGC CACTGTTGAT TGATGAAGAT 
TACTATGCTA GAGTGGAAGG CCGAGAGATT ATTTCTAATT TGGCAAAGGC TGCTGGTCTG 
GCTACTATGA TTTCTACCAT GAGACCTGAT ATAGATAACA TGGATGAATA TGTCCGAAAC 
ACAACAGCTA GAGCTTTTGC TGTCGTAGCC TCTGCCCTGG GCATCCCTTC TTTATTGCCT 
TTCTTAAAAG CTGTGTGCAA AAGCAAGAAG TCTTGGCAAG CGAGGCACAC TGGTATTAAG 
ATTGTACAAC AGATAGCCAT TCTCATGGGC TGTGCTATCT TGCCACATCT CAGAAGTTTG 
GTTGAAATCA TTGAACATGG TCTCGTGGAT GAGCAGCAGA AAGTTCGGAC CATCAGTGCT 
CTGGCCATTG CTGCTTTGGC TGAGGCAGCA ACTCCTTATG GTATTGAGTC TTTTGATTCT 
GTGCTCAAGC CCCTATGGAA GGGTATCCGC CAACACAGAG GAAAGGGTCT GGCTGCTTTC 
TTGAAGGCTA TTGGGTATCT TATTCCTCTT ATGGATGCAG AATATGCCAA CTACTATACT 
AGAGAAGTGA TGTTAATCCT CATTCGAGAA TTTCAGTCTC CTGATGAAGA AATGAAGAAA 
ATTGTTTTGA AGGTGGTGAA ACAGTGTTGT GGAACTGATG GTGTAGAAGC AAACTACATT 
AAAACAGAGA TTCTTCCACC TTTCTTTAAA CACTTTTGGC AACACAGAAT GGCTTTGGAT 
AGAAGAAATT ACCGACAGTT AGTTGATACA ACTGTGGAGT TGGCAAACAA AGTAGGTGCA 
GCAGAGATCA TATCTAGGAT TGTTGATGAT CTGAAGGATG AAGCTGAGCA GTACAGGAAA 
ATGGTGATGG AAACAATTGA GAAAATTATG GGTAATCTAG GCGCAGCAGA TATTGACCAT 
AAACTTGAGG AACAACTGAT TGATGGTATT CTCTATGCTT TCCAAGAACA GACTACAGAG 
GATTCAGTAA TGTTGAATGG CTTTGGCACA GTAGTTAATG CTCTTGGCAA ACGAGTCAAA 
CCATACTTGC CTCAGATCTG TGGTACAGTT TTATGGCGTT TAAATAACAA ATCAGCAAAA 
GTTAGGCAAC AAGCAGCTGA CTTGATTTCT CGAACTGCTG TTGTTATGAA GACTTGTCAA 
GAGGAAAAAC TGATGGGGCA CTTGGGTGTG GTGCTGTATG AATATTTGGG TGAAGAGTAC 
CCTGAAGTAT TGGGCAGCAT TCTTGGAGCA CTGAAGGCTA TTGTAAACGT AATAGGTATG 
CATAAGATGA CACCACCAAT TAAAGATCTC CTGCCTAGAC TCACCCCCAT TTTAAAGAAC 
AGACATGAAA AAGTACAAGA GAATTGTATT GATCTTGTTG GACGCATTGC TGACAGGGGA 
GCTGAATATG TATCTGCAAG AGAATGGATG AGGATTTGCT TTGAGCTTTT AGAGCTCTTA 
AAAGCTCACA AAAAAGCTAT TCGAAGAGCT ACCGTCAATA CATTTGGTTA TATCGCAAAG 
GCTATTGGCC CTCATGATGT ATTGGCTACA CTTTTAAACA ACCTCAAAGT TCAGGAAAGG 
CAGAACAGGG TTTGTACGAC TGTAGCCATA GCCATTGTTG CTGAAACATG CTCCCCCTTC 
ACAGTACTGC CTGCCTTAAT GAATGAATAC AGAGTTCCTG AACTTAATGT GCAAAATGGA 
GTGTTAAAAT CACTCTCCTT CTTGTTTGAG TATATCGGCG AAATGGGGAA AGACTACATC 
TATGCTGTGA CGCCTTTACT TGAGGATGCG TTAATGGACA GGGACCTTGT TCACAGACAG 
ACAGCGAGTG CAGTGGTGCA GCACATGTCA CTTGGTGTTT ACGGATTTGG CTGTGAGGAT 
TCTCTGAATC ACTTACTGAA CTATGTGTGG CCTAATGTGT TTGAGACATC TCCCCATGTA 
ATCCAGGCAG TTATGGGAGC TCTGGAGGGC CTGAGGGTTG CTATTGGACC ATGCAGAATG 
TTGCAGTATT GCTTACAGGG TCTGTTCCAC CCAGCTCGGA AAGTCAGAGA CGTATATTGG 
AAAATTTACA ACTCCATCTA CATTGGCTCC CAGGATGCTC TCATAGCACA TTACCCACGA 
ATCTACAATG ACGATAAGAA CACCTATATT CGTTACGAAC TTGACTATAT CTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099596.1
CDS	1975..5451
Translation	MNARTYMDVMREQHLTKEEREIRQQLAEKAKAGELKVVNGAAASQPPSKRKRRWDQTADQTPGATPKKLSSWDQAETPGHTPSLRWDETPGRAKGSETPGATPGSKIWDPTPSHTPAGAATPGRGDTPGHATPGHGGATSSARKNRWDETPKTERDTPGHGSGWAETPRTDRGGDSIGETPTPGASKRKSRWDETPASQMGGSTPVLTPGKTPIGTPAMNMATPTPGHIMSMTPEQLQAWRWEREIDERNRPLSDEELDAMFPEGYKVLPPPAGYVPIRTPARKLTATPTPLGGMTGFHMQTEDRTMKSVNDQPSGNLPFLKPDDIQYFDKLLVDVDESTLSPEEQKERKIMKLLLKIKNGTPPMRKAALRQITDKAREFGAGPLFNQILPLLMSPTLEDQERHLLVKVIDRILYKLDDLVRPYVHKILVVIEPLLIDEDYYARVEGREIISNLAKAAGLATMISTMRPDIDNMDEYVRNTTARAFAVVASALGIPSLLPFLKAVCKSKKSWQARHTGIKIVQQIAILMGCAILPHLRSLVEIIEHGLVDEQQKVRTISALAIAALAEAATPYGIESFDSVLKPLWKGIRQHRGKGLAAFLKAIGYLIPLMDAEYANYYTREVMLILIREFQSPDEEMKKIVLKVVKQCCGTDGVEANYIKTEILPPFFKHFWQHRMALDRRNYRQLVDTTVELANKVGAAEIISRIVDDLKDEAEQYRKMVMETIEKIMGNLGAADIDHKLEEQLIDGILYAFQEQTTEDSVMLNGFGTVVNALGKRVKPYLPQICGTVLWRLNNKSAKVRQQAADLISRTAVVMKTCQEEKLMGHLGVVLYEYLGEEYPEVLGSILGALKAIVNVIGMHKMTPPIKDLLPRLTPILKNRHEKVQENCIDLVGRIADRGAEYVSAREWMRICFELLELLKAHKKAIRRATVNTFGYIAKAIGPHDVLATLLNNLKVQERQNRVCTTVAIAIVAETCSPFTVLPALMNEYRVPELNVQNGVLKSLSFLFEYIGEMGKDYIYAVTPLLEDALMDRDLVHRQTASAVVQHMSLGVYGFGCEDSLNHLLNYVWPNVFETSPHVIQAVMGALEGLRVAIGPCRMLQYCLQGLFHPARKVRDVYWKIYNSIYIGSQDALIAHYPRIYNDDKNTYIRYELDYIL

Target ORF information:

RefSeq Version	XM_030243736.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus splicing factor 3b, subunit 1 (Sf3b1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243736.1

ORF Insert Sequence:

ATGAATGCTA GAACCTACAT GGATGTTATG CGAGAACAAC ATTTGACTAA GGAAGAGAGA 
GAAATTAGGC AACAACTAGC AGAAAAAGCT AAAGCTGGAG AACTAAAAGT CGTCAATGGA 
GCAGCAGCAT CCCAGCCTCC CTCAAAACGA AAACGGCGCT GGGATCAGAC CGCTGACCAG 
ACTCCTGGTG CCACTCCCAA AAAGCTATCG AGTTGGGATC AGGCAGAGAC CCCTGGGCAT 
ACCCCATCTT TAAGATGGGA TGAGACACCG GGTCGTGCAA AAGGAAGTGA AACACCTGGC 
GCAACCCCAG GCTCAAAAAT ATGGGACCCT ACACCGAGTC ATACACCTGC GGGAGCTGCT 
ACTCCTGGGC GAGGCGACAC ACCAGGCCAT GCAACCCCGG GCCATGGTGG TGCAACTTCC 
AGTGCTCGTA AAAACAGATG GGATGAGACC CCCAAAACAG AAAGAGATAC TCCTGGGCAT 
GGAAGTGGAT GGGCTGAGAC TCCTCGAACA GATCGAGGTG GAGACTCTAT TGGTGAAACA 
CCAACTCCTG GAGCAAGTAA AAGAAAGTCT CGTTGGGATG AAACGCCGGC GAGTCAAATG 
GGTGGAAGCA CTCCTGTTCT GACTCCAGGA AAAACACCAA TTGGCACACC AGCCATGAAC 
ATGGCTACCC CGACTCCAGG TCACATAATG AGCATGACAC CTGAACAGCT TCAGGCGTGG 
CGGTGGGAGA GAGAAATTGA TGAACGAAAC CGCCCACTTT CTGATGAAGA ATTAGATGCT 
ATGTTCCCAG AAGGATATAA GGTACTTCCC CCTCCAGCTG GTTATGTTCC TATTCGAACT 
CCAGCTCGAA AGCTGACAGC AACTCCTACA CCTTTGGGTG GTATGACTGG TTTCCACATG 
CAAACTGAAG ACAGAACTAT GAAAAGTGTC AATGACCAGC CATCTGGGAA TCTTCCATTT 
TTAAAACCTG ATGACATTCA GTACTTTGAT AAACTATTGG TTGATGTAGA TGAATCAACA 
CTTAGTCCAG AAGAACAAAA AGAAAGAAAA ATAATGAAGT TGCTTTTAAA AATTAAGAAT 
GGTACACCTC CAATGAGAAA GGCTGCCTTA CGTCAGATTA CTGATAAAGC TCGAGAATTT 
GGAGCTGGAC CACTGTTTAA CCAGATTCTT CCTCTGTTGA TGTCCCCTAC ACTTGAGGAT 
CAAGAGCGAC ATTTACTTGT GAAAGTTATT GATAGAATAT TGTATAAACT TGATGACTTG 
GTGCGGCCGT ATGTGCACAA GATTCTTGTG GTTATCGAGC CACTGTTGAT TGATGAAGAT 
TACTATGCTA GAGTGGAAGG CCGAGAGATT ATTTCTAATT TGGCAAAGGC TGCTGGTCTG 
GCTACTATGA TTTCTACCAT GAGACCTGAT ATAGATAACA TGGATGAATA TGTCCGAAAC 
ACAACAGCTA GAGCTTTTGC TGTCGTAGCC TCTGCCCTGG GCATCCCTTC TTTATTGCCT 
TTCTTAAAAG CTGTGTGCAA AAGCAAGAAG TCTTGGCAAG CGAGGCACAC TGGTATTAAG 
ATTGTACAAC AGATAGCCAT TCTCATGGGC TGTGCTATCT TGCCACATCT CAGAAGTTTG 
GTTGAAATCA TTGAACATGG TCTCGTGGAT GAGCAGCAGA AAGTTCGGAC CATCAGTGCT 
CTGGCCATTG CTGCTTTGGC TGAGGCAGCA ACTCCTTATG GTATTGAGTC TTTTGATTCT 
GTGCTCAAGC CCCTATGGAA GGGTATCCGC CAACACAGAG GAAAGGGTCT GGCTGCTTTC 
TTGAAGGCTA TTGGGTATCT TATTCCTCTT ATGGATGCAG AATATGCCAA CTACTATACT 
AGAGAAGTGA TGTTAATCCT CATTCGAGAA TTTCAGTCTC CTGATGAAGA AATGAAGAAA 
ATTGTTTTGA AGGTGGTGAA ACAGTGTTGT GGAACTGATG GTGTAGAAGC AAACTACATT 
AAAACAGAGA TTCTTCCACC TTTCTTTAAA CACTTTTGGC AACACAGAAT GGCTTTGGAT 
AGAAGAAATT ACCGACAGTT AGTTGATACA ACTGTGGAGT TGGCAAACAA AGTAGGTGCA 
GCAGAGATCA TATCTAGGAT TGTTGATGAT CTGAAGGATG AAGCTGAGCA GTACAGGAAA 
ATGGTGATGG AAACAATTGA GAAAATTATG GGTAATCTAG GCGCAGCAGA TATTGACCAT 
AAACTTGAGG AACAACTGAT TGATGGTATT CTCTATGCTT TCCAAGAACA GACTACAGAG 
GATTCAGTAA TGTTGAATGG CTTTGGCACA GTAGTTAATG CTCTTGGCAA ACGAGTCAAA 
CCATACTTGC CTCAGATCTG TGGTACAGTT TTATGGCGTT TAAATAACAA ATCAGCAAAA 
GTTAGGCAAC AAGCAGCTGA CTTGATTTCT CGAACTGCTG TTGTTATGAA GACTTGTCAA 
GAGGAAAAAC TGATGGGGCA CTTGGGTGTG GTGCTGTATG AATATTTGGG TGAAGAGTAC 
CCTGAAGTAT TGGGCAGCAT TCTTGGAGCA CTGAAGGCTA TTGTAAACGT AATAGGTATG 
CATAAGATGA CACCACCAAT TAAAGATCTC CTGCCTAGAC TCACCCCCAT TTTAAAGAAC 
AGACATGAAA AAGTACAAGA GAATTGTATT GATCTTGTTG GACGCATTGC TGACAGGGGA 
GCTGAATATG TATCTGCAAG AGAATGGATG AGGATTTGCT TTGAGCTTTT AGAGCTCTTA 
AAAGCTCACA AAAAAGCTAT TCGAAGAGCT ACCGTCAATA CATTTGGTTA TATCGCAAAG 
GCTATTGGCC CTCATGATGT ATTGGCTACA CTTTTAAACA ACCTCAAAGT TCAGGAAAGG 
CAGAACAGGG TTTGTACGAC TGTAGCCATA GCCATTGTTG CTGAAACATG CTCCCCCTTC 
ACAGTACTGC CTGCCTTAAT GAATGAATAC AGAGTTCCTG AACTTAATGT GCAAAATGGA 
GTGTTAAAAT CACTCTCCTT CTTGTTTGAG TATATCGGCG AAATGGGGAA AGACTACATC 
TATGCTGTGA CGCCTTTACT TGAGGATGCG TTAATGGACA GGGACCTTGT TCACAGACAG 
ACAGCGAGTG CAGTGGTGCA GCACATGTCA CTTGGTGTTT ACGGATTTGG CTGTGAGGAT 
TCTCTGAATC ACTTACTGAA CTATGTGTGG CCTAATGTGT TTGAGACATC TCCCCATGTA 
ATCCAGGCAG TTATGGGAGC TCTGGAGGGC CTGAGGGTTG CTATTGGACC ATGCAGAATG 
TTGCAGTATT GCTTACAGGG TCTGTTCCAC CCAGCTCGGA AAGTCAGAGA CGTATATTGG 
AAAATTTACA ACTCCATCTA CATTGGCTCC CAGGATGCTC TCATAGCACA TTACCCACGA 
ATCTACAATG ACGATAAGAA CACCTATATT CGTTACGAAC TTGACTATAT CTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sf3b1 ( NM_031179.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_112456.2 Mus musculus splicing factor 3b, subunit 1 (Sf3b1), mRNA.

Price & Availability↑

CloneID	OMu20984
Clone ID Related Accession (Same CDS sequence)	NM_031179.2
Accession Version	NM_031179.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-07
Organism	Mus musculus(house mouse)
Product	splicing factor 3B subunit 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK331410.1, AK140982.1, CN526934.1, CF952155.1, AK048521.1, AK087700.1 and AK077993.1. On Jul 25, 2007 this sequence version replaced NM_031179.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB037890.1, SRR1660815.221636.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164141, SAMN01164142 [ECO:0000348] ##Evidence-Data-END##

ATGGCGAAGA TCGCCAAGAC TCACGAAGAT ATCGAAGCAC AGATTCGAGA AATTCAAGGC 
AAGAAGGCAG CTCTTGATGA AGCCCAAGGA GTGGGCCTTG ATTCCACAGG TTATTATGAC 
CAAGAAATTT ATGGTGGAAG TGATAGCAGA TTTGCTGGAT ATGTGACATC AATTGCTGCA 
ACTGAACTTG AAGATGATGA CGATGACTAC TCATCATCCA CGAGCTTGCT CGGTCAGAAG 
AAGCCTGGAT ATCATGCCCC CGTGGCGTTG CTTAATGATA TACCACAGTC AACAGAGCAG 
TATGATCCAT TTGCTGAGCA TCGTCCTCCA AAGATTGCCG ATCGGGAAGA TGAATACAAA 
AAGCATAGGC GGACCATGAT AATTTCCCCA GAGCGTCTTG ATCCTTTTGC AGATGGAGGG 
AAGACTCCCG ATCCTAAAAT GAATGCTAGA ACCTACATGG ATGTTATGCG AGAACAACAT 
TTGACTAAGG AAGAGAGAGA AATTAGGCAA CAACTAGCAG AAAAAGCTAA AGCTGGAGAA 
CTAAAAGTCG TCAATGGAGC AGCAGCATCC CAGCCTCCCT CAAAACGAAA ACGGCGCTGG 
GATCAGACCG CTGACCAGAC TCCTGGTGCC ACTCCCAAAA AGCTATCGAG TTGGGATCAG 
GCAGAGACCC CTGGGCATAC CCCATCTTTA AGATGGGATG AGACACCGGG TCGTGCAAAA 
GGAAGTGAAA CACCTGGCGC AACCCCAGGC TCAAAAATAT GGGACCCTAC ACCGAGTCAT 
ACACCTGCGG GAGCTGCTAC TCCTGGGCGA GGCGACACAC CAGGCCATGC AACCCCGGGC 
CATGGTGGTG CAACTTCCAG TGCTCGTAAA AACAGATGGG ATGAGACCCC CAAAACAGAA 
AGAGATACTC CTGGGCATGG AAGTGGATGG GCTGAGACTC CTCGAACAGA TCGAGGTGGA 
GACTCTATTG GTGAAACACC AACTCCTGGA GCAAGTAAAA GAAAGTCTCG TTGGGATGAA 
ACGCCGGCGA GTCAAATGGG TGGAAGCACT CCTGTTCTGA CTCCAGGAAA AACACCAATT 
GGCACACCAG CCATGAACAT GGCTACCCCG ACTCCAGGTC ACATAATGAG CATGACACCT 
GAACAGCTTC AGGCGTGGCG GTGGGAGAGA GAAATTGATG AACGAAACCG CCCACTTTCT 
GATGAAGAAT TAGATGCTAT GTTCCCAGAA GGATATAAGG TACTTCCCCC TCCAGCTGGT 
TATGTTCCTA TTCGAACTCC AGCTCGAAAG CTGACAGCAA CTCCTACACC TTTGGGTGGT 
ATGACTGGTT TCCACATGCA AACTGAAGAC AGAACTATGA AAAGTGTCAA TGACCAGCCA 
TCTGGGAATC TTCCATTTTT AAAACCTGAT GACATTCAGT ACTTTGATAA ACTATTGGTT 
GATGTAGATG AATCAACACT TAGTCCAGAA GAACAAAAAG AAAGAAAAAT AATGAAGTTG 
CTTTTAAAAA TTAAGAATGG TACACCTCCA ATGAGAAAGG CTGCCTTACG TCAGATTACT 
GATAAAGCTC GAGAATTTGG AGCTGGACCA CTGTTTAACC AGATTCTTCC TCTGTTGATG 
TCCCCTACAC TTGAGGATCA AGAGCGACAT TTACTTGTGA AAGTTATTGA TAGAATATTG 
TATAAACTTG ATGACTTGGT GCGGCCGTAT GTGCACAAGA TTCTTGTGGT TATCGAGCCA 
CTGTTGATTG ATGAAGATTA CTATGCTAGA GTGGAAGGCC GAGAGATTAT TTCTAATTTG 
GCAAAGGCTG CTGGTCTGGC TACTATGATT TCTACCATGA GACCTGATAT AGATAACATG 
GATGAATATG TCCGAAACAC AACAGCTAGA GCTTTTGCTG TCGTAGCCTC TGCCCTGGGC 
ATCCCTTCTT TATTGCCTTT CTTAAAAGCT GTGTGCAAAA GCAAGAAGTC TTGGCAAGCG 
AGGCACACTG GTATTAAGAT TGTACAACAG ATAGCCATTC TCATGGGCTG TGCTATCTTG 
CCACATCTCA GAAGTTTGGT TGAAATCATT GAACATGGTC TCGTGGATGA GCAGCAGAAA 
GTTCGGACCA TCAGTGCTCT GGCCATTGCT GCTTTGGCTG AGGCAGCAAC TCCTTATGGT 
ATTGAGTCTT TTGATTCTGT GCTCAAGCCC CTATGGAAGG GTATCCGCCA ACACAGAGGA 
AAGGGTCTGG CTGCTTTCTT GAAGGCTATT GGGTATCTTA TTCCTCTTAT GGATGCAGAA 
TATGCCAACT ACTATACTAG AGAAGTGATG TTAATCCTCA TTCGAGAATT TCAGTCTCCT 
GATGAAGAAA TGAAGAAAAT TGTTTTGAAG GTGGTGAAAC AGTGTTGTGG AACTGATGGT 
GTAGAAGCAA ACTACATTAA AACAGAGATT CTTCCACCTT TCTTTAAACA CTTTTGGCAA 
CACAGAATGG CTTTGGATAG AAGAAATTAC CGACAGTTAG TTGATACAAC TGTGGAGTTG 
GCAAACAAAG TAGGTGCAGC AGAGATCATA TCTAGGATTG TTGATGATCT GAAGGATGAA 
GCTGAGCAGT ACAGGAAAAT GGTGATGGAA ACAATTGAGA AAATTATGGG TAATCTAGGC 
GCAGCAGATA TTGACCATAA ACTTGAGGAA CAACTGATTG ATGGTATTCT CTATGCTTTC 
CAAGAACAGA CTACAGAGGA TTCAGTAATG TTGAATGGCT TTGGCACAGT AGTTAATGCT 
CTTGGCAAAC GAGTCAAACC ATACTTGCCT CAGATCTGTG GTACAGTTTT ATGGCGTTTA 
AATAACAAAT CAGCAAAAGT TAGGCAACAA GCAGCTGACT TGATTTCTCG AACTGCTGTT 
GTTATGAAGA CTTGTCAAGA GGAAAAACTG ATGGGGCACT TGGGTGTGGT GCTGTATGAA 
TATTTGGGTG AAGAGTACCC TGAAGTATTG GGCAGCATTC TTGGAGCACT GAAGGCTATT 
GTAAACGTAA TAGGTATGCA TAAGATGACA CCACCAATTA AAGATCTCCT GCCTAGACTC 
ACCCCCATTT TAAAGAACAG ACATGAAAAA GTACAAGAGA ATTGTATTGA TCTTGTTGGA 
CGCATTGCTG ACAGGGGAGC TGAATATGTA TCTGCAAGAG AATGGATGAG GATTTGCTTT 
GAGCTTTTAG AGCTCTTAAA AGCTCACAAA AAAGCTATTC GAAGAGCTAC CGTCAATACA 
TTTGGTTATA TCGCAAAGGC TATTGGCCCT CATGATGTAT TGGCTACACT TTTAAACAAC 
CTCAAAGTTC AGGAAAGGCA GAACAGGGTT TGTACGACTG TAGCCATAGC CATTGTTGCT 
GAAACATGCT CCCCCTTCAC AGTACTGCCT GCCTTAATGA ATGAATACAG AGTTCCTGAA 
CTTAATGTGC AAAATGGAGT GTTAAAATCA CTCTCCTTCT TGTTTGAGTA TATCGGCGAA 
ATGGGGAAAG ACTACATCTA TGCTGTGACG CCTTTACTTG AGGATGCGTT AATGGACAGG 
GACCTTGTTC ACAGACAGAC AGCGAGTGCA GTGGTGCAGC ACATGTCACT TGGTGTTTAC 
GGATTTGGCT GTGAGGATTC TCTGAATCAC TTACTGAACT ATGTGTGGCC TAATGTGTTT 
GAGACATCTC CCCATGTAAT CCAGGCAGTT ATGGGAGCTC TGGAGGGCCT GAGGGTTGCT 
ATTGGACCAT GCAGAATGTT GCAGTATTGC TTACAGGGTC TGTTCCACCC AGCTCGGAAA 
GTCAGAGACG TATATTGGAA AATTTACAAC TCCATCTACA TTGGCTCCCA GGATGCTCTC 
ATAGCACATT ACCCACGAAT CTACAATGAC GATAAGAACA CCTATATTCG TTACGAACTT 
GACTATATCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112456.2
CDS	113..4027
Translation	MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTMIISPERLDPFADGGKTPDPKMNARTYMDVMREQHLTKEEREIRQQLAEKAKAGELKVVNGAAASQPPSKRKRRWDQTADQTPGATPKKLSSWDQAETPGHTPSLRWDETPGRAKGSETPGATPGSKIWDPTPSHTPAGAATPGRGDTPGHATPGHGGATSSARKNRWDETPKTERDTPGHGSGWAETPRTDRGGDSIGETPTPGASKRKSRWDETPASQMGGSTPVLTPGKTPIGTPAMNMATPTPGHIMSMTPEQLQAWRWEREIDERNRPLSDEELDAMFPEGYKVLPPPAGYVPIRTPARKLTATPTPLGGMTGFHMQTEDRTMKSVNDQPSGNLPFLKPDDIQYFDKLLVDVDESTLSPEEQKERKIMKLLLKIKNGTPPMRKAALRQITDKAREFGAGPLFNQILPLLMSPTLEDQERHLLVKVIDRILYKLDDLVRPYVHKILVVIEPLLIDEDYYARVEGREIISNLAKAAGLATMISTMRPDIDNMDEYVRNTTARAFAVVASALGIPSLLPFLKAVCKSKKSWQARHTGIKIVQQIAILMGCAILPHLRSLVEIIEHGLVDEQQKVRTISALAIAALAEAATPYGIESFDSVLKPLWKGIRQHRGKGLAAFLKAIGYLIPLMDAEYANYYTREVMLILIREFQSPDEEMKKIVLKVVKQCCGTDGVEANYIKTEILPPFFKHFWQHRMALDRRNYRQLVDTTVELANKVGAAEIISRIVDDLKDEAEQYRKMVMETIEKIMGNLGAADIDHKLEEQLIDGILYAFQEQTTEDSVMLNGFGTVVNALGKRVKPYLPQICGTVLWRLNNKSAKVRQQAADLISRTAVVMKTCQEEKLMGHLGVVLYEYLGEEYPEVLGSILGALKAIVNVIGMHKMTPPIKDLLPRLTPILKNRHEKVQENCIDLVGRIADRGAEYVSAREWMRICFELLELLKAHKKAIRRATVNTFGYIAKAIGPHDVLATLLNNLKVQERQNRVCTTVAIAIVAETCSPFTVLPALMNEYRVPELNVQNGVLKSLSFLFEYIGEMGKDYIYAVTPLLEDALMDRDLVHRQTASAVVQHMSLGVYGFGCEDSLNHLLNYVWPNVFETSPHVIQAVMGALEGLRVAIGPCRMLQYCLQGLFHPARKVRDVYWKIYNSIYIGSQDALIAHYPRIYNDDKNTYIRYELDYIL

Target ORF information:

RefSeq Version	NM_031179.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus splicing factor 3b, subunit 1 (Sf3b1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031179.2

ORF Insert Sequence:

ATGGCGAAGA TCGCCAAGAC TCACGAAGAT ATCGAAGCAC AGATTCGAGA AATTCAAGGC 
AAGAAGGCAG CTCTTGATGA AGCCCAAGGA GTGGGCCTTG ATTCCACAGG TTATTATGAC 
CAAGAAATTT ATGGTGGAAG TGATAGCAGA TTTGCTGGAT ATGTGACATC AATTGCTGCA 
ACTGAACTTG AAGATGATGA CGATGACTAC TCATCATCCA CGAGCTTGCT CGGTCAGAAG 
AAGCCTGGAT ATCATGCCCC CGTGGCGTTG CTTAATGATA TACCACAGTC AACAGAGCAG 
TATGATCCAT TTGCTGAGCA TCGTCCTCCA AAGATTGCCG ATCGGGAAGA TGAATACAAA 
AAGCATAGGC GGACCATGAT AATTTCCCCA GAGCGTCTTG ATCCTTTTGC AGATGGAGGG 
AAGACTCCCG ATCCTAAAAT GAATGCTAGA ACCTACATGG ATGTTATGCG AGAACAACAT 
TTGACTAAGG AAGAGAGAGA AATTAGGCAA CAACTAGCAG AAAAAGCTAA AGCTGGAGAA 
CTAAAAGTCG TCAATGGAGC AGCAGCATCC CAGCCTCCCT CAAAACGAAA ACGGCGCTGG 
GATCAGACCG CTGACCAGAC TCCTGGTGCC ACTCCCAAAA AGCTATCGAG TTGGGATCAG 
GCAGAGACCC CTGGGCATAC CCCATCTTTA AGATGGGATG AGACACCGGG TCGTGCAAAA 
GGAAGTGAAA CACCTGGCGC AACCCCAGGC TCAAAAATAT GGGACCCTAC ACCGAGTCAT 
ACACCTGCGG GAGCTGCTAC TCCTGGGCGA GGCGACACAC CAGGCCATGC AACCCCGGGC 
CATGGTGGTG CAACTTCCAG TGCTCGTAAA AACAGATGGG ATGAGACCCC CAAAACAGAA 
AGAGATACTC CTGGGCATGG AAGTGGATGG GCTGAGACTC CTCGAACAGA TCGAGGTGGA 
GACTCTATTG GTGAAACACC AACTCCTGGA GCAAGTAAAA GAAAGTCTCG TTGGGATGAA 
ACGCCGGCGA GTCAAATGGG TGGAAGCACT CCTGTTCTGA CTCCAGGAAA AACACCAATT 
GGCACACCAG CCATGAACAT GGCTACCCCG ACTCCAGGTC ACATAATGAG CATGACACCT 
GAACAGCTTC AGGCGTGGCG GTGGGAGAGA GAAATTGATG AACGAAACCG CCCACTTTCT 
GATGAAGAAT TAGATGCTAT GTTCCCAGAA GGATATAAGG TACTTCCCCC TCCAGCTGGT 
TATGTTCCTA TTCGAACTCC AGCTCGAAAG CTGACAGCAA CTCCTACACC TTTGGGTGGT 
ATGACTGGTT TCCACATGCA AACTGAAGAC AGAACTATGA AAAGTGTCAA TGACCAGCCA 
TCTGGGAATC TTCCATTTTT AAAACCTGAT GACATTCAGT ACTTTGATAA ACTATTGGTT 
GATGTAGATG AATCAACACT TAGTCCAGAA GAACAAAAAG AAAGAAAAAT AATGAAGTTG 
CTTTTAAAAA TTAAGAATGG TACACCTCCA ATGAGAAAGG CTGCCTTACG TCAGATTACT 
GATAAAGCTC GAGAATTTGG AGCTGGACCA CTGTTTAACC AGATTCTTCC TCTGTTGATG 
TCCCCTACAC TTGAGGATCA AGAGCGACAT TTACTTGTGA AAGTTATTGA TAGAATATTG 
TATAAACTTG ATGACTTGGT GCGGCCGTAT GTGCACAAGA TTCTTGTGGT TATCGAGCCA 
CTGTTGATTG ATGAAGATTA CTATGCTAGA GTGGAAGGCC GAGAGATTAT TTCTAATTTG 
GCAAAGGCTG CTGGTCTGGC TACTATGATT TCTACCATGA GACCTGATAT AGATAACATG 
GATGAATATG TCCGAAACAC AACAGCTAGA GCTTTTGCTG TCGTAGCCTC TGCCCTGGGC 
ATCCCTTCTT TATTGCCTTT CTTAAAAGCT GTGTGCAAAA GCAAGAAGTC TTGGCAAGCG 
AGGCACACTG GTATTAAGAT TGTACAACAG ATAGCCATTC TCATGGGCTG TGCTATCTTG 
CCACATCTCA GAAGTTTGGT TGAAATCATT GAACATGGTC TCGTGGATGA GCAGCAGAAA 
GTTCGGACCA TCAGTGCTCT GGCCATTGCT GCTTTGGCTG AGGCAGCAAC TCCTTATGGT 
ATTGAGTCTT TTGATTCTGT GCTCAAGCCC CTATGGAAGG GTATCCGCCA ACACAGAGGA 
AAGGGTCTGG CTGCTTTCTT GAAGGCTATT GGGTATCTTA TTCCTCTTAT GGATGCAGAA 
TATGCCAACT ACTATACTAG AGAAGTGATG TTAATCCTCA TTCGAGAATT TCAGTCTCCT 
GATGAAGAAA TGAAGAAAAT TGTTTTGAAG GTGGTGAAAC AGTGTTGTGG AACTGATGGT 
GTAGAAGCAA ACTACATTAA AACAGAGATT CTTCCACCTT TCTTTAAACA CTTTTGGCAA 
CACAGAATGG CTTTGGATAG AAGAAATTAC CGACAGTTAG TTGATACAAC TGTGGAGTTG 
GCAAACAAAG TAGGTGCAGC AGAGATCATA TCTAGGATTG TTGATGATCT GAAGGATGAA 
GCTGAGCAGT ACAGGAAAAT GGTGATGGAA ACAATTGAGA AAATTATGGG TAATCTAGGC 
GCAGCAGATA TTGACCATAA ACTTGAGGAA CAACTGATTG ATGGTATTCT CTATGCTTTC 
CAAGAACAGA CTACAGAGGA TTCAGTAATG TTGAATGGCT TTGGCACAGT AGTTAATGCT 
CTTGGCAAAC GAGTCAAACC ATACTTGCCT CAGATCTGTG GTACAGTTTT ATGGCGTTTA 
AATAACAAAT CAGCAAAAGT TAGGCAACAA GCAGCTGACT TGATTTCTCG AACTGCTGTT 
GTTATGAAGA CTTGTCAAGA GGAAAAACTG ATGGGGCACT TGGGTGTGGT GCTGTATGAA 
TATTTGGGTG AAGAGTACCC TGAAGTATTG GGCAGCATTC TTGGAGCACT GAAGGCTATT 
GTAAACGTAA TAGGTATGCA TAAGATGACA CCACCAATTA AAGATCTCCT GCCTAGACTC 
ACCCCCATTT TAAAGAACAG ACATGAAAAA GTACAAGAGA ATTGTATTGA TCTTGTTGGA 
CGCATTGCTG ACAGGGGAGC TGAATATGTA TCTGCAAGAG AATGGATGAG GATTTGCTTT 
GAGCTTTTAG AGCTCTTAAA AGCTCACAAA AAAGCTATTC GAAGAGCTAC CGTCAATACA 
TTTGGTTATA TCGCAAAGGC TATTGGCCCT CATGATGTAT TGGCTACACT TTTAAACAAC 
CTCAAAGTTC AGGAAAGGCA GAACAGGGTT TGTACGACTG TAGCCATAGC CATTGTTGCT 
GAAACATGCT CCCCCTTCAC AGTACTGCCT GCCTTAATGA ATGAATACAG AGTTCCTGAA 
CTTAATGTGC AAAATGGAGT GTTAAAATCA CTCTCCTTCT TGTTTGAGTA TATCGGCGAA 
ATGGGGAAAG ACTACATCTA TGCTGTGACG CCTTTACTTG AGGATGCGTT AATGGACAGG 
GACCTTGTTC ACAGACAGAC AGCGAGTGCA GTGGTGCAGC ACATGTCACT TGGTGTTTAC 
GGATTTGGCT GTGAGGATTC TCTGAATCAC TTACTGAACT ATGTGTGGCC TAATGTGTTT 
GAGACATCTC CCCATGTAAT CCAGGCAGTT ATGGGAGCTC TGGAGGGCCT GAGGGTTGCT 
ATTGGACCAT GCAGAATGTT GCAGTATTGC TTACAGGGTC TGTTCCACCC AGCTCGGAAA 
GTCAGAGACG TATATTGGAA AATTTACAAC TCCATCTACA TTGGCTCCCA GGATGCTCTC 
ATAGCACATT ACCCACGAAT CTACAATGAC GATAAGAACA CCTATATTCG TTACGAACTT 
GACTATATCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sf3b1 ( XM_017312972.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168461.1 Mus musculus splicing factor 3b, subunit 1 (Sf3b1), transcript variant X1, mRNA.

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CloneID	OMu27668
Clone ID Related Accession (Same CDS sequence)	XM_030243736.1 , XM_017312972.1 , XM_017312973.1
Accession Version	XM_017312972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	splicing factor 3B subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCTA GAACCTACAT GGATGTTATG CGAGAACAAC ATTTGACTAA GGAAGAGAGA 
GAAATTAGGC AACAACTAGC AGAAAAAGCT AAAGCTGGAG AACTAAAAGT CGTCAATGGA 
GCAGCAGCAT CCCAGCCTCC CTCAAAACGA AAACGGCGCT GGGATCAGAC CGCTGACCAG 
ACTCCTGGTG CCACTCCCAA AAAGCTATCG AGTTGGGATC AGGCAGAGAC CCCTGGGCAT 
ACCCCATCTT TAAGATGGGA TGAGACACCG GGTCGTGCAA AAGGAAGTGA AACACCTGGC 
GCAACCCCAG GCTCAAAAAT ATGGGACCCT ACACCGAGTC ATACACCTGC GGGAGCTGCT 
ACTCCTGGGC GAGGCGACAC ACCAGGCCAT GCAACCCCGG GCCATGGTGG TGCAACTTCC 
AGTGCTCGTA AAAACAGATG GGATGAGACC CCCAAAACAG AAAGAGATAC TCCTGGGCAT 
GGAAGTGGAT GGGCTGAGAC TCCTCGAACA GATCGAGGTG GAGACTCTAT TGGTGAAACA 
CCAACTCCTG GAGCAAGTAA AAGAAAGTCT CGTTGGGATG AAACGCCGGC GAGTCAAATG 
GGTGGAAGCA CTCCTGTTCT GACTCCAGGA AAAACACCAA TTGGCACACC AGCCATGAAC 
ATGGCTACCC CGACTCCAGG TCACATAATG AGCATGACAC CTGAACAGCT TCAGGCGTGG 
CGGTGGGAGA GAGAAATTGA TGAACGAAAC CGCCCACTTT CTGATGAAGA ATTAGATGCT 
ATGTTCCCAG AAGGATATAA GGTACTTCCC CCTCCAGCTG GTTATGTTCC TATTCGAACT 
CCAGCTCGAA AGCTGACAGC AACTCCTACA CCTTTGGGTG GTATGACTGG TTTCCACATG 
CAAACTGAAG ACAGAACTAT GAAAAGTGTC AATGACCAGC CATCTGGGAA TCTTCCATTT 
TTAAAACCTG ATGACATTCA GTACTTTGAT AAACTATTGG TTGATGTAGA TGAATCAACA 
CTTAGTCCAG AAGAACAAAA AGAAAGAAAA ATAATGAAGT TGCTTTTAAA AATTAAGAAT 
GGTACACCTC CAATGAGAAA GGCTGCCTTA CGTCAGATTA CTGATAAAGC TCGAGAATTT 
GGAGCTGGAC CACTGTTTAA CCAGATTCTT CCTCTGTTGA TGTCCCCTAC ACTTGAGGAT 
CAAGAGCGAC ATTTACTTGT GAAAGTTATT GATAGAATAT TGTATAAACT TGATGACTTG 
GTGCGGCCGT ATGTGCACAA GATTCTTGTG GTTATCGAGC CACTGTTGAT TGATGAAGAT 
TACTATGCTA GAGTGGAAGG CCGAGAGATT ATTTCTAATT TGGCAAAGGC TGCTGGTCTG 
GCTACTATGA TTTCTACCAT GAGACCTGAT ATAGATAACA TGGATGAATA TGTCCGAAAC 
ACAACAGCTA GAGCTTTTGC TGTCGTAGCC TCTGCCCTGG GCATCCCTTC TTTATTGCCT 
TTCTTAAAAG CTGTGTGCAA AAGCAAGAAG TCTTGGCAAG CGAGGCACAC TGGTATTAAG 
ATTGTACAAC AGATAGCCAT TCTCATGGGC TGTGCTATCT TGCCACATCT CAGAAGTTTG 
GTTGAAATCA TTGAACATGG TCTCGTGGAT GAGCAGCAGA AAGTTCGGAC CATCAGTGCT 
CTGGCCATTG CTGCTTTGGC TGAGGCAGCA ACTCCTTATG GTATTGAGTC TTTTGATTCT 
GTGCTCAAGC CCCTATGGAA GGGTATCCGC CAACACAGAG GAAAGGGTCT GGCTGCTTTC 
TTGAAGGCTA TTGGGTATCT TATTCCTCTT ATGGATGCAG AATATGCCAA CTACTATACT 
AGAGAAGTGA TGTTAATCCT CATTCGAGAA TTTCAGTCTC CTGATGAAGA AATGAAGAAA 
ATTGTTTTGA AGGTGGTGAA ACAGTGTTGT GGAACTGATG GTGTAGAAGC AAACTACATT 
AAAACAGAGA TTCTTCCACC TTTCTTTAAA CACTTTTGGC AACACAGAAT GGCTTTGGAT 
AGAAGAAATT ACCGACAGTT AGTTGATACA ACTGTGGAGT TGGCAAACAA AGTAGGTGCA 
GCAGAGATCA TATCTAGGAT TGTTGATGAT CTGAAGGATG AAGCTGAGCA GTACAGGAAA 
ATGGTGATGG AAACAATTGA GAAAATTATG GGTAATCTAG GCGCAGCAGA TATTGACCAT 
AAACTTGAGG AACAACTGAT TGATGGTATT CTCTATGCTT TCCAAGAACA GACTACAGAG 
GATTCAGTAA TGTTGAATGG CTTTGGCACA GTAGTTAATG CTCTTGGCAA ACGAGTCAAA 
CCATACTTGC CTCAGATCTG TGGTACAGTT TTATGGCGTT TAAATAACAA ATCAGCAAAA 
GTTAGGCAAC AAGCAGCTGA CTTGATTTCT CGAACTGCTG TTGTTATGAA GACTTGTCAA 
GAGGAAAAAC TGATGGGGCA CTTGGGTGTG GTGCTGTATG AATATTTGGG TGAAGAGTAC 
CCTGAAGTAT TGGGCAGCAT TCTTGGAGCA CTGAAGGCTA TTGTAAACGT AATAGGTATG 
CATAAGATGA CACCACCAAT TAAAGATCTC CTGCCTAGAC TCACCCCCAT TTTAAAGAAC 
AGACATGAAA AAGTACAAGA GAATTGTATT GATCTTGTTG GACGCATTGC TGACAGGGGA 
GCTGAATATG TATCTGCAAG AGAATGGATG AGGATTTGCT TTGAGCTTTT AGAGCTCTTA 
AAAGCTCACA AAAAAGCTAT TCGAAGAGCT ACCGTCAATA CATTTGGTTA TATCGCAAAG 
GCTATTGGCC CTCATGATGT ATTGGCTACA CTTTTAAACA ACCTCAAAGT TCAGGAAAGG 
CAGAACAGGG TTTGTACGAC TGTAGCCATA GCCATTGTTG CTGAAACATG CTCCCCCTTC 
ACAGTACTGC CTGCCTTAAT GAATGAATAC AGAGTTCCTG AACTTAATGT GCAAAATGGA 
GTGTTAAAAT CACTCTCCTT CTTGTTTGAG TATATCGGCG AAATGGGGAA AGACTACATC 
TATGCTGTGA CGCCTTTACT TGAGGATGCG TTAATGGACA GGGACCTTGT TCACAGACAG 
ACAGCGAGTG CAGTGGTGCA GCACATGTCA CTTGGTGTTT ACGGATTTGG CTGTGAGGAT 
TCTCTGAATC ACTTACTGAA CTATGTGTGG CCTAATGTGT TTGAGACATC TCCCCATGTA 
ATCCAGGCAG TTATGGGAGC TCTGGAGGGC CTGAGGGTTG CTATTGGACC ATGCAGAATG 
TTGCAGTATT GCTTACAGGG TCTGTTCCAC CCAGCTCGGA AAGTCAGAGA CGTATATTGG 
AAAATTTACA ACTCCATCTA CATTGGCTCC CAGGATGCTC TCATAGCACA TTACCCACGA 
ATCTACAATG ACGATAAGAA CACCTATATT CGTTACGAAC TTGACTATAT CTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168461.1
CDS	1760..5236
Translation	MNARTYMDVMREQHLTKEEREIRQQLAEKAKAGELKVVNGAAASQPPSKRKRRWDQTADQTPGATPKKLSSWDQAETPGHTPSLRWDETPGRAKGSETPGATPGSKIWDPTPSHTPAGAATPGRGDTPGHATPGHGGATSSARKNRWDETPKTERDTPGHGSGWAETPRTDRGGDSIGETPTPGASKRKSRWDETPASQMGGSTPVLTPGKTPIGTPAMNMATPTPGHIMSMTPEQLQAWRWEREIDERNRPLSDEELDAMFPEGYKVLPPPAGYVPIRTPARKLTATPTPLGGMTGFHMQTEDRTMKSVNDQPSGNLPFLKPDDIQYFDKLLVDVDESTLSPEEQKERKIMKLLLKIKNGTPPMRKAALRQITDKAREFGAGPLFNQILPLLMSPTLEDQERHLLVKVIDRILYKLDDLVRPYVHKILVVIEPLLIDEDYYARVEGREIISNLAKAAGLATMISTMRPDIDNMDEYVRNTTARAFAVVASALGIPSLLPFLKAVCKSKKSWQARHTGIKIVQQIAILMGCAILPHLRSLVEIIEHGLVDEQQKVRTISALAIAALAEAATPYGIESFDSVLKPLWKGIRQHRGKGLAAFLKAIGYLIPLMDAEYANYYTREVMLILIREFQSPDEEMKKIVLKVVKQCCGTDGVEANYIKTEILPPFFKHFWQHRMALDRRNYRQLVDTTVELANKVGAAEIISRIVDDLKDEAEQYRKMVMETIEKIMGNLGAADIDHKLEEQLIDGILYAFQEQTTEDSVMLNGFGTVVNALGKRVKPYLPQICGTVLWRLNNKSAKVRQQAADLISRTAVVMKTCQEEKLMGHLGVVLYEYLGEEYPEVLGSILGALKAIVNVIGMHKMTPPIKDLLPRLTPILKNRHEKVQENCIDLVGRIADRGAEYVSAREWMRICFELLELLKAHKKAIRRATVNTFGYIAKAIGPHDVLATLLNNLKVQERQNRVCTTVAIAIVAETCSPFTVLPALMNEYRVPELNVQNGVLKSLSFLFEYIGEMGKDYIYAVTPLLEDALMDRDLVHRQTASAVVQHMSLGVYGFGCEDSLNHLLNYVWPNVFETSPHVIQAVMGALEGLRVAIGPCRMLQYCLQGLFHPARKVRDVYWKIYNSIYIGSQDALIAHYPRIYNDDKNTYIRYELDYIL

Target ORF information:

RefSeq Version	XM_017312972.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus splicing factor 3b, subunit 1 (Sf3b1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312972.1

ORF Insert Sequence:

ATGAATGCTA GAACCTACAT GGATGTTATG CGAGAACAAC ATTTGACTAA GGAAGAGAGA 
GAAATTAGGC AACAACTAGC AGAAAAAGCT AAAGCTGGAG AACTAAAAGT CGTCAATGGA 
GCAGCAGCAT CCCAGCCTCC CTCAAAACGA AAACGGCGCT GGGATCAGAC CGCTGACCAG 
ACTCCTGGTG CCACTCCCAA AAAGCTATCG AGTTGGGATC AGGCAGAGAC CCCTGGGCAT 
ACCCCATCTT TAAGATGGGA TGAGACACCG GGTCGTGCAA AAGGAAGTGA AACACCTGGC 
GCAACCCCAG GCTCAAAAAT ATGGGACCCT ACACCGAGTC ATACACCTGC GGGAGCTGCT 
ACTCCTGGGC GAGGCGACAC ACCAGGCCAT GCAACCCCGG GCCATGGTGG TGCAACTTCC 
AGTGCTCGTA AAAACAGATG GGATGAGACC CCCAAAACAG AAAGAGATAC TCCTGGGCAT 
GGAAGTGGAT GGGCTGAGAC TCCTCGAACA GATCGAGGTG GAGACTCTAT TGGTGAAACA 
CCAACTCCTG GAGCAAGTAA AAGAAAGTCT CGTTGGGATG AAACGCCGGC GAGTCAAATG 
GGTGGAAGCA CTCCTGTTCT GACTCCAGGA AAAACACCAA TTGGCACACC AGCCATGAAC 
ATGGCTACCC CGACTCCAGG TCACATAATG AGCATGACAC CTGAACAGCT TCAGGCGTGG 
CGGTGGGAGA GAGAAATTGA TGAACGAAAC CGCCCACTTT CTGATGAAGA ATTAGATGCT 
ATGTTCCCAG AAGGATATAA GGTACTTCCC CCTCCAGCTG GTTATGTTCC TATTCGAACT 
CCAGCTCGAA AGCTGACAGC AACTCCTACA CCTTTGGGTG GTATGACTGG TTTCCACATG 
CAAACTGAAG ACAGAACTAT GAAAAGTGTC AATGACCAGC CATCTGGGAA TCTTCCATTT 
TTAAAACCTG ATGACATTCA GTACTTTGAT AAACTATTGG TTGATGTAGA TGAATCAACA 
CTTAGTCCAG AAGAACAAAA AGAAAGAAAA ATAATGAAGT TGCTTTTAAA AATTAAGAAT 
GGTACACCTC CAATGAGAAA GGCTGCCTTA CGTCAGATTA CTGATAAAGC TCGAGAATTT 
GGAGCTGGAC CACTGTTTAA CCAGATTCTT CCTCTGTTGA TGTCCCCTAC ACTTGAGGAT 
CAAGAGCGAC ATTTACTTGT GAAAGTTATT GATAGAATAT TGTATAAACT TGATGACTTG 
GTGCGGCCGT ATGTGCACAA GATTCTTGTG GTTATCGAGC CACTGTTGAT TGATGAAGAT 
TACTATGCTA GAGTGGAAGG CCGAGAGATT ATTTCTAATT TGGCAAAGGC TGCTGGTCTG 
GCTACTATGA TTTCTACCAT GAGACCTGAT ATAGATAACA TGGATGAATA TGTCCGAAAC 
ACAACAGCTA GAGCTTTTGC TGTCGTAGCC TCTGCCCTGG GCATCCCTTC TTTATTGCCT 
TTCTTAAAAG CTGTGTGCAA AAGCAAGAAG TCTTGGCAAG CGAGGCACAC TGGTATTAAG 
ATTGTACAAC AGATAGCCAT TCTCATGGGC TGTGCTATCT TGCCACATCT CAGAAGTTTG 
GTTGAAATCA TTGAACATGG TCTCGTGGAT GAGCAGCAGA AAGTTCGGAC CATCAGTGCT 
CTGGCCATTG CTGCTTTGGC TGAGGCAGCA ACTCCTTATG GTATTGAGTC TTTTGATTCT 
GTGCTCAAGC CCCTATGGAA GGGTATCCGC CAACACAGAG GAAAGGGTCT GGCTGCTTTC 
TTGAAGGCTA TTGGGTATCT TATTCCTCTT ATGGATGCAG AATATGCCAA CTACTATACT 
AGAGAAGTGA TGTTAATCCT CATTCGAGAA TTTCAGTCTC CTGATGAAGA AATGAAGAAA 
ATTGTTTTGA AGGTGGTGAA ACAGTGTTGT GGAACTGATG GTGTAGAAGC AAACTACATT 
AAAACAGAGA TTCTTCCACC TTTCTTTAAA CACTTTTGGC AACACAGAAT GGCTTTGGAT 
AGAAGAAATT ACCGACAGTT AGTTGATACA ACTGTGGAGT TGGCAAACAA AGTAGGTGCA 
GCAGAGATCA TATCTAGGAT TGTTGATGAT CTGAAGGATG AAGCTGAGCA GTACAGGAAA 
ATGGTGATGG AAACAATTGA GAAAATTATG GGTAATCTAG GCGCAGCAGA TATTGACCAT 
AAACTTGAGG AACAACTGAT TGATGGTATT CTCTATGCTT TCCAAGAACA GACTACAGAG 
GATTCAGTAA TGTTGAATGG CTTTGGCACA GTAGTTAATG CTCTTGGCAA ACGAGTCAAA 
CCATACTTGC CTCAGATCTG TGGTACAGTT TTATGGCGTT TAAATAACAA ATCAGCAAAA 
GTTAGGCAAC AAGCAGCTGA CTTGATTTCT CGAACTGCTG TTGTTATGAA GACTTGTCAA 
GAGGAAAAAC TGATGGGGCA CTTGGGTGTG GTGCTGTATG AATATTTGGG TGAAGAGTAC 
CCTGAAGTAT TGGGCAGCAT TCTTGGAGCA CTGAAGGCTA TTGTAAACGT AATAGGTATG 
CATAAGATGA CACCACCAAT TAAAGATCTC CTGCCTAGAC TCACCCCCAT TTTAAAGAAC 
AGACATGAAA AAGTACAAGA GAATTGTATT GATCTTGTTG GACGCATTGC TGACAGGGGA 
GCTGAATATG TATCTGCAAG AGAATGGATG AGGATTTGCT TTGAGCTTTT AGAGCTCTTA 
AAAGCTCACA AAAAAGCTAT TCGAAGAGCT ACCGTCAATA CATTTGGTTA TATCGCAAAG 
GCTATTGGCC CTCATGATGT ATTGGCTACA CTTTTAAACA ACCTCAAAGT TCAGGAAAGG 
CAGAACAGGG TTTGTACGAC TGTAGCCATA GCCATTGTTG CTGAAACATG CTCCCCCTTC 
ACAGTACTGC CTGCCTTAAT GAATGAATAC AGAGTTCCTG AACTTAATGT GCAAAATGGA 
GTGTTAAAAT CACTCTCCTT CTTGTTTGAG TATATCGGCG AAATGGGGAA AGACTACATC 
TATGCTGTGA CGCCTTTACT TGAGGATGCG TTAATGGACA GGGACCTTGT TCACAGACAG 
ACAGCGAGTG CAGTGGTGCA GCACATGTCA CTTGGTGTTT ACGGATTTGG CTGTGAGGAT 
TCTCTGAATC ACTTACTGAA CTATGTGTGG CCTAATGTGT TTGAGACATC TCCCCATGTA 
ATCCAGGCAG TTATGGGAGC TCTGGAGGGC CTGAGGGTTG CTATTGGACC ATGCAGAATG 
TTGCAGTATT GCTTACAGGG TCTGTTCCAC CCAGCTCGGA AAGTCAGAGA CGTATATTGG 
AAAATTTACA ACTCCATCTA CATTGGCTCC CAGGATGCTC TCATAGCACA TTACCCACGA 
ATCTACAATG ACGATAAGAA CACCTATATT CGTTACGAAC TTGACTATAT CTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sf3b1 ( XM_017312973.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168462.1 Mus musculus splicing factor 3b, subunit 1 (Sf3b1), transcript variant X2, mRNA.

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CloneID	OMu27668
Clone ID Related Accession (Same CDS sequence)	XM_030243736.1 , XM_017312972.1 , XM_017312973.1
Accession Version	XM_017312973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	splicing factor 3B subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCTA GAACCTACAT GGATGTTATG CGAGAACAAC ATTTGACTAA GGAAGAGAGA 
GAAATTAGGC AACAACTAGC AGAAAAAGCT AAAGCTGGAG AACTAAAAGT CGTCAATGGA 
GCAGCAGCAT CCCAGCCTCC CTCAAAACGA AAACGGCGCT GGGATCAGAC CGCTGACCAG 
ACTCCTGGTG CCACTCCCAA AAAGCTATCG AGTTGGGATC AGGCAGAGAC CCCTGGGCAT 
ACCCCATCTT TAAGATGGGA TGAGACACCG GGTCGTGCAA AAGGAAGTGA AACACCTGGC 
GCAACCCCAG GCTCAAAAAT ATGGGACCCT ACACCGAGTC ATACACCTGC GGGAGCTGCT 
ACTCCTGGGC GAGGCGACAC ACCAGGCCAT GCAACCCCGG GCCATGGTGG TGCAACTTCC 
AGTGCTCGTA AAAACAGATG GGATGAGACC CCCAAAACAG AAAGAGATAC TCCTGGGCAT 
GGAAGTGGAT GGGCTGAGAC TCCTCGAACA GATCGAGGTG GAGACTCTAT TGGTGAAACA 
CCAACTCCTG GAGCAAGTAA AAGAAAGTCT CGTTGGGATG AAACGCCGGC GAGTCAAATG 
GGTGGAAGCA CTCCTGTTCT GACTCCAGGA AAAACACCAA TTGGCACACC AGCCATGAAC 
ATGGCTACCC CGACTCCAGG TCACATAATG AGCATGACAC CTGAACAGCT TCAGGCGTGG 
CGGTGGGAGA GAGAAATTGA TGAACGAAAC CGCCCACTTT CTGATGAAGA ATTAGATGCT 
ATGTTCCCAG AAGGATATAA GGTACTTCCC CCTCCAGCTG GTTATGTTCC TATTCGAACT 
CCAGCTCGAA AGCTGACAGC AACTCCTACA CCTTTGGGTG GTATGACTGG TTTCCACATG 
CAAACTGAAG ACAGAACTAT GAAAAGTGTC AATGACCAGC CATCTGGGAA TCTTCCATTT 
TTAAAACCTG ATGACATTCA GTACTTTGAT AAACTATTGG TTGATGTAGA TGAATCAACA 
CTTAGTCCAG AAGAACAAAA AGAAAGAAAA ATAATGAAGT TGCTTTTAAA AATTAAGAAT 
GGTACACCTC CAATGAGAAA GGCTGCCTTA CGTCAGATTA CTGATAAAGC TCGAGAATTT 
GGAGCTGGAC CACTGTTTAA CCAGATTCTT CCTCTGTTGA TGTCCCCTAC ACTTGAGGAT 
CAAGAGCGAC ATTTACTTGT GAAAGTTATT GATAGAATAT TGTATAAACT TGATGACTTG 
GTGCGGCCGT ATGTGCACAA GATTCTTGTG GTTATCGAGC CACTGTTGAT TGATGAAGAT 
TACTATGCTA GAGTGGAAGG CCGAGAGATT ATTTCTAATT TGGCAAAGGC TGCTGGTCTG 
GCTACTATGA TTTCTACCAT GAGACCTGAT ATAGATAACA TGGATGAATA TGTCCGAAAC 
ACAACAGCTA GAGCTTTTGC TGTCGTAGCC TCTGCCCTGG GCATCCCTTC TTTATTGCCT 
TTCTTAAAAG CTGTGTGCAA AAGCAAGAAG TCTTGGCAAG CGAGGCACAC TGGTATTAAG 
ATTGTACAAC AGATAGCCAT TCTCATGGGC TGTGCTATCT TGCCACATCT CAGAAGTTTG 
GTTGAAATCA TTGAACATGG TCTCGTGGAT GAGCAGCAGA AAGTTCGGAC CATCAGTGCT 
CTGGCCATTG CTGCTTTGGC TGAGGCAGCA ACTCCTTATG GTATTGAGTC TTTTGATTCT 
GTGCTCAAGC CCCTATGGAA GGGTATCCGC CAACACAGAG GAAAGGGTCT GGCTGCTTTC 
TTGAAGGCTA TTGGGTATCT TATTCCTCTT ATGGATGCAG AATATGCCAA CTACTATACT 
AGAGAAGTGA TGTTAATCCT CATTCGAGAA TTTCAGTCTC CTGATGAAGA AATGAAGAAA 
ATTGTTTTGA AGGTGGTGAA ACAGTGTTGT GGAACTGATG GTGTAGAAGC AAACTACATT 
AAAACAGAGA TTCTTCCACC TTTCTTTAAA CACTTTTGGC AACACAGAAT GGCTTTGGAT 
AGAAGAAATT ACCGACAGTT AGTTGATACA ACTGTGGAGT TGGCAAACAA AGTAGGTGCA 
GCAGAGATCA TATCTAGGAT TGTTGATGAT CTGAAGGATG AAGCTGAGCA GTACAGGAAA 
ATGGTGATGG AAACAATTGA GAAAATTATG GGTAATCTAG GCGCAGCAGA TATTGACCAT 
AAACTTGAGG AACAACTGAT TGATGGTATT CTCTATGCTT TCCAAGAACA GACTACAGAG 
GATTCAGTAA TGTTGAATGG CTTTGGCACA GTAGTTAATG CTCTTGGCAA ACGAGTCAAA 
CCATACTTGC CTCAGATCTG TGGTACAGTT TTATGGCGTT TAAATAACAA ATCAGCAAAA 
GTTAGGCAAC AAGCAGCTGA CTTGATTTCT CGAACTGCTG TTGTTATGAA GACTTGTCAA 
GAGGAAAAAC TGATGGGGCA CTTGGGTGTG GTGCTGTATG AATATTTGGG TGAAGAGTAC 
CCTGAAGTAT TGGGCAGCAT TCTTGGAGCA CTGAAGGCTA TTGTAAACGT AATAGGTATG 
CATAAGATGA CACCACCAAT TAAAGATCTC CTGCCTAGAC TCACCCCCAT TTTAAAGAAC 
AGACATGAAA AAGTACAAGA GAATTGTATT GATCTTGTTG GACGCATTGC TGACAGGGGA 
GCTGAATATG TATCTGCAAG AGAATGGATG AGGATTTGCT TTGAGCTTTT AGAGCTCTTA 
AAAGCTCACA AAAAAGCTAT TCGAAGAGCT ACCGTCAATA CATTTGGTTA TATCGCAAAG 
GCTATTGGCC CTCATGATGT ATTGGCTACA CTTTTAAACA ACCTCAAAGT TCAGGAAAGG 
CAGAACAGGG TTTGTACGAC TGTAGCCATA GCCATTGTTG CTGAAACATG CTCCCCCTTC 
ACAGTACTGC CTGCCTTAAT GAATGAATAC AGAGTTCCTG AACTTAATGT GCAAAATGGA 
GTGTTAAAAT CACTCTCCTT CTTGTTTGAG TATATCGGCG AAATGGGGAA AGACTACATC 
TATGCTGTGA CGCCTTTACT TGAGGATGCG TTAATGGACA GGGACCTTGT TCACAGACAG 
ACAGCGAGTG CAGTGGTGCA GCACATGTCA CTTGGTGTTT ACGGATTTGG CTGTGAGGAT 
TCTCTGAATC ACTTACTGAA CTATGTGTGG CCTAATGTGT TTGAGACATC TCCCCATGTA 
ATCCAGGCAG TTATGGGAGC TCTGGAGGGC CTGAGGGTTG CTATTGGACC ATGCAGAATG 
TTGCAGTATT GCTTACAGGG TCTGTTCCAC CCAGCTCGGA AAGTCAGAGA CGTATATTGG 
AAAATTTACA ACTCCATCTA CATTGGCTCC CAGGATGCTC TCATAGCACA TTACCCACGA 
ATCTACAATG ACGATAAGAA CACCTATATT CGTTACGAAC TTGACTATAT CTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168462.1
CDS	512..3988
Translation	MNARTYMDVMREQHLTKEEREIRQQLAEKAKAGELKVVNGAAASQPPSKRKRRWDQTADQTPGATPKKLSSWDQAETPGHTPSLRWDETPGRAKGSETPGATPGSKIWDPTPSHTPAGAATPGRGDTPGHATPGHGGATSSARKNRWDETPKTERDTPGHGSGWAETPRTDRGGDSIGETPTPGASKRKSRWDETPASQMGGSTPVLTPGKTPIGTPAMNMATPTPGHIMSMTPEQLQAWRWEREIDERNRPLSDEELDAMFPEGYKVLPPPAGYVPIRTPARKLTATPTPLGGMTGFHMQTEDRTMKSVNDQPSGNLPFLKPDDIQYFDKLLVDVDESTLSPEEQKERKIMKLLLKIKNGTPPMRKAALRQITDKAREFGAGPLFNQILPLLMSPTLEDQERHLLVKVIDRILYKLDDLVRPYVHKILVVIEPLLIDEDYYARVEGREIISNLAKAAGLATMISTMRPDIDNMDEYVRNTTARAFAVVASALGIPSLLPFLKAVCKSKKSWQARHTGIKIVQQIAILMGCAILPHLRSLVEIIEHGLVDEQQKVRTISALAIAALAEAATPYGIESFDSVLKPLWKGIRQHRGKGLAAFLKAIGYLIPLMDAEYANYYTREVMLILIREFQSPDEEMKKIVLKVVKQCCGTDGVEANYIKTEILPPFFKHFWQHRMALDRRNYRQLVDTTVELANKVGAAEIISRIVDDLKDEAEQYRKMVMETIEKIMGNLGAADIDHKLEEQLIDGILYAFQEQTTEDSVMLNGFGTVVNALGKRVKPYLPQICGTVLWRLNNKSAKVRQQAADLISRTAVVMKTCQEEKLMGHLGVVLYEYLGEEYPEVLGSILGALKAIVNVIGMHKMTPPIKDLLPRLTPILKNRHEKVQENCIDLVGRIADRGAEYVSAREWMRICFELLELLKAHKKAIRRATVNTFGYIAKAIGPHDVLATLLNNLKVQERQNRVCTTVAIAIVAETCSPFTVLPALMNEYRVPELNVQNGVLKSLSFLFEYIGEMGKDYIYAVTPLLEDALMDRDLVHRQTASAVVQHMSLGVYGFGCEDSLNHLLNYVWPNVFETSPHVIQAVMGALEGLRVAIGPCRMLQYCLQGLFHPARKVRDVYWKIYNSIYIGSQDALIAHYPRIYNDDKNTYIRYELDYIL

Target ORF information:

RefSeq Version	XM_017312973.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus splicing factor 3b, subunit 1 (Sf3b1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312973.1

ORF Insert Sequence:

ATGAATGCTA GAACCTACAT GGATGTTATG CGAGAACAAC ATTTGACTAA GGAAGAGAGA 
GAAATTAGGC AACAACTAGC AGAAAAAGCT AAAGCTGGAG AACTAAAAGT CGTCAATGGA 
GCAGCAGCAT CCCAGCCTCC CTCAAAACGA AAACGGCGCT GGGATCAGAC CGCTGACCAG 
ACTCCTGGTG CCACTCCCAA AAAGCTATCG AGTTGGGATC AGGCAGAGAC CCCTGGGCAT 
ACCCCATCTT TAAGATGGGA TGAGACACCG GGTCGTGCAA AAGGAAGTGA AACACCTGGC 
GCAACCCCAG GCTCAAAAAT ATGGGACCCT ACACCGAGTC ATACACCTGC GGGAGCTGCT 
ACTCCTGGGC GAGGCGACAC ACCAGGCCAT GCAACCCCGG GCCATGGTGG TGCAACTTCC 
AGTGCTCGTA AAAACAGATG GGATGAGACC CCCAAAACAG AAAGAGATAC TCCTGGGCAT 
GGAAGTGGAT GGGCTGAGAC TCCTCGAACA GATCGAGGTG GAGACTCTAT TGGTGAAACA 
CCAACTCCTG GAGCAAGTAA AAGAAAGTCT CGTTGGGATG AAACGCCGGC GAGTCAAATG 
GGTGGAAGCA CTCCTGTTCT GACTCCAGGA AAAACACCAA TTGGCACACC AGCCATGAAC 
ATGGCTACCC CGACTCCAGG TCACATAATG AGCATGACAC CTGAACAGCT TCAGGCGTGG 
CGGTGGGAGA GAGAAATTGA TGAACGAAAC CGCCCACTTT CTGATGAAGA ATTAGATGCT 
ATGTTCCCAG AAGGATATAA GGTACTTCCC CCTCCAGCTG GTTATGTTCC TATTCGAACT 
CCAGCTCGAA AGCTGACAGC AACTCCTACA CCTTTGGGTG GTATGACTGG TTTCCACATG 
CAAACTGAAG ACAGAACTAT GAAAAGTGTC AATGACCAGC CATCTGGGAA TCTTCCATTT 
TTAAAACCTG ATGACATTCA GTACTTTGAT AAACTATTGG TTGATGTAGA TGAATCAACA 
CTTAGTCCAG AAGAACAAAA AGAAAGAAAA ATAATGAAGT TGCTTTTAAA AATTAAGAAT 
GGTACACCTC CAATGAGAAA GGCTGCCTTA CGTCAGATTA CTGATAAAGC TCGAGAATTT 
GGAGCTGGAC CACTGTTTAA CCAGATTCTT CCTCTGTTGA TGTCCCCTAC ACTTGAGGAT 
CAAGAGCGAC ATTTACTTGT GAAAGTTATT GATAGAATAT TGTATAAACT TGATGACTTG 
GTGCGGCCGT ATGTGCACAA GATTCTTGTG GTTATCGAGC CACTGTTGAT TGATGAAGAT 
TACTATGCTA GAGTGGAAGG CCGAGAGATT ATTTCTAATT TGGCAAAGGC TGCTGGTCTG 
GCTACTATGA TTTCTACCAT GAGACCTGAT ATAGATAACA TGGATGAATA TGTCCGAAAC 
ACAACAGCTA GAGCTTTTGC TGTCGTAGCC TCTGCCCTGG GCATCCCTTC TTTATTGCCT 
TTCTTAAAAG CTGTGTGCAA AAGCAAGAAG TCTTGGCAAG CGAGGCACAC TGGTATTAAG 
ATTGTACAAC AGATAGCCAT TCTCATGGGC TGTGCTATCT TGCCACATCT CAGAAGTTTG 
GTTGAAATCA TTGAACATGG TCTCGTGGAT GAGCAGCAGA AAGTTCGGAC CATCAGTGCT 
CTGGCCATTG CTGCTTTGGC TGAGGCAGCA ACTCCTTATG GTATTGAGTC TTTTGATTCT 
GTGCTCAAGC CCCTATGGAA GGGTATCCGC CAACACAGAG GAAAGGGTCT GGCTGCTTTC 
TTGAAGGCTA TTGGGTATCT TATTCCTCTT ATGGATGCAG AATATGCCAA CTACTATACT 
AGAGAAGTGA TGTTAATCCT CATTCGAGAA TTTCAGTCTC CTGATGAAGA AATGAAGAAA 
ATTGTTTTGA AGGTGGTGAA ACAGTGTTGT GGAACTGATG GTGTAGAAGC AAACTACATT 
AAAACAGAGA TTCTTCCACC TTTCTTTAAA CACTTTTGGC AACACAGAAT GGCTTTGGAT 
AGAAGAAATT ACCGACAGTT AGTTGATACA ACTGTGGAGT TGGCAAACAA AGTAGGTGCA 
GCAGAGATCA TATCTAGGAT TGTTGATGAT CTGAAGGATG AAGCTGAGCA GTACAGGAAA 
ATGGTGATGG AAACAATTGA GAAAATTATG GGTAATCTAG GCGCAGCAGA TATTGACCAT 
AAACTTGAGG AACAACTGAT TGATGGTATT CTCTATGCTT TCCAAGAACA GACTACAGAG 
GATTCAGTAA TGTTGAATGG CTTTGGCACA GTAGTTAATG CTCTTGGCAA ACGAGTCAAA 
CCATACTTGC CTCAGATCTG TGGTACAGTT TTATGGCGTT TAAATAACAA ATCAGCAAAA 
GTTAGGCAAC AAGCAGCTGA CTTGATTTCT CGAACTGCTG TTGTTATGAA GACTTGTCAA 
GAGGAAAAAC TGATGGGGCA CTTGGGTGTG GTGCTGTATG AATATTTGGG TGAAGAGTAC 
CCTGAAGTAT TGGGCAGCAT TCTTGGAGCA CTGAAGGCTA TTGTAAACGT AATAGGTATG 
CATAAGATGA CACCACCAAT TAAAGATCTC CTGCCTAGAC TCACCCCCAT TTTAAAGAAC 
AGACATGAAA AAGTACAAGA GAATTGTATT GATCTTGTTG GACGCATTGC TGACAGGGGA 
GCTGAATATG TATCTGCAAG AGAATGGATG AGGATTTGCT TTGAGCTTTT AGAGCTCTTA 
AAAGCTCACA AAAAAGCTAT TCGAAGAGCT ACCGTCAATA CATTTGGTTA TATCGCAAAG 
GCTATTGGCC CTCATGATGT ATTGGCTACA CTTTTAAACA ACCTCAAAGT TCAGGAAAGG 
CAGAACAGGG TTTGTACGAC TGTAGCCATA GCCATTGTTG CTGAAACATG CTCCCCCTTC 
ACAGTACTGC CTGCCTTAAT GAATGAATAC AGAGTTCCTG AACTTAATGT GCAAAATGGA 
GTGTTAAAAT CACTCTCCTT CTTGTTTGAG TATATCGGCG AAATGGGGAA AGACTACATC 
TATGCTGTGA CGCCTTTACT TGAGGATGCG TTAATGGACA GGGACCTTGT TCACAGACAG 
ACAGCGAGTG CAGTGGTGCA GCACATGTCA CTTGGTGTTT ACGGATTTGG CTGTGAGGAT 
TCTCTGAATC ACTTACTGAA CTATGTGTGG CCTAATGTGT TTGAGACATC TCCCCATGTA 
ATCCAGGCAG TTATGGGAGC TCTGGAGGGC CTGAGGGTTG CTATTGGACC ATGCAGAATG 
TTGCAGTATT GCTTACAGGG TCTGTTCCAC CCAGCTCGGA AAGTCAGAGA CGTATATTGG 
AAAATTTACA ACTCCATCTA CATTGGCTCC CAGGATGCTC TCATAGCACA TTACCCACGA 
ATCTACAATG ACGATAAGAA CACCTATATT CGTTACGAAC TTGACTATAT CTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.
Scientific reports637396(2016 Nov)
Cui W,Dai X,Marcho C,Han Z,Zhang K,Tremblay KD,Mager J

Physiologic Expression of Sf3b1(K700E) Causes Impaired Erythropoiesis, Aberrant Splicing, and Sensitivity to Therapeutic Spliceosome Modulation.
Cancer cell30(3)404-417(2016 Sep)
Obeng EA,Chappell RJ,Seiler M,Chen MC,Campagna DR,Schmidt PJ,Schneider RK,Lord AM,Wang L,Gambe RG,McConkey ME,Ali AM,Raza A,Yu L,Buonamici S,Smith PG,Mullally A,Wu CJ,Fleming MD,Ebert BL

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Sf3b1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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