Ift122 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ift122
Entrez Gene ID	81896
Full Name	intraflagellar transport 122
Synonyms	C86139,Wdr10,sopb
General protein information	Preferred Names intraflagellar transport 122 Names intraflagellar transport protein 122 homolog WD repeat domain 10 WD repeat-containing protein 10 intraflagellar transport 122 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 E3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006506799.4	XP_006506862.1	intraflagellar transport protein 122 homolog isoform X3
NM_031177.4	NP_112454.2	intraflagellar transport protein 122 homolog isoform 1
XM_006506799.3	XP_006506862.1	intraflagellar transport protein 122 homolog isoform X3
XM_006506797.2	XP_006506860.1	intraflagellar transport protein 122 homolog isoform X1
XM_006506798.2	XP_006506861.1	intraflagellar transport protein 122 homolog isoform X2
NM_001167763.1	NP_001161235.1	intraflagellar transport protein 122 homolog isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ift122	NP_112454.2
Caenorhabditis elegans (roundworm)	daf-10	NP_501591.2
Xenopus tropicalis (tropical clawed frog)	ift122	NP_001106492.1
Pan troglodytes (chimpanzee)	IFT122	XP_516739.2
Rattus norvegicus (Norway rat)	Ift122	XP_006237129.1
Drosophila melanogaster (fruit fly)	Oseg1	NP_648221.3
Homo sapiens (human)	IFT122	NP_443715.1
Canis lupus familiaris (dog)	IFT122	XP_005632188.1
	IFT122	XP_002798754.1
Bos taurus (cattle)	IFT122	NP_001193521.1
Gallus gallus (chicken)	IFT122	XP_414454.4
Danio rerio (zebrafish)	ift122	NP_998055.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006535	XP_316573.2

Gene Ontology
Bibliography

Related articles in PubMed

Proper ciliary assembly is critical for restricting Hedgehog signaling during early eye development in mice.
Burnett JB, Lupu FI, Eggenschwiler JT
Developmental biology430(1)32-40(2017 10)

ATMIN is a transcriptional regulator of both lung morphogenesis and ciliogenesis.
Goggolidou P, Stevens JL, Agueci F, Keynton J, Wheway G, Grimes DT, Patel SH, Hilton H, Morthorst SK, DiPaolo A, Williams DJ, Sanderson J, Khoronenkova SV, Powles-Glover N, Ermakov A, Esapa CT, Romero R, Dianov GL, Briscoe J, Johnson CA, Pedersen LB, Norris DP
Development (Cambridge, England)141(20)3966-77(2014 Oct)

What are those cilia doing in the neural tube?
Bay SN, Caspary T
Cilia1(1)19(2012 Oct)

The IFT-A complex regulates Shh signaling through cilia structure and membrane protein trafficking.
Liem KF Jr, Ashe A, He M, Satir P, Moran J, Beier D, Wicking C, Anderson KV
The Journal of cell biology197(6)789-800(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that IFT122 controls the ciliary localization of Shh pathway regulators in different ways.
Title: Intraflagellar transport protein 122 antagonizes Sonic Hedgehog signaling and controls ciliary localization of pathway components.

Studies on a complex A mutant mouse, defective for the Ift122 gene, is reported.
Title: Defective ciliogenesis, embryonic lethality and severe impairment of the Sonic Hedgehog pathway caused by inactivation of the mouse complex A intraflagellar transport gene Ift122/Wdr10, partially overlapping with the DNA repair gene Med1/Mbd4.

The following Ift122 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ift122 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu37694	XM_006506799.4 Latest version!	Mus musculus intraflagellar transport 122 (Ift122), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu08494	NM_031177.4 Latest version!	Mus musculus intraflagellar transport 122 (Ift122), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu11119	NM_001167763.1 Latest version!	Mus musculus intraflagellar transport 122 (Ift122), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu37692	XM_006506797.2 Latest version!	Mus musculus intraflagellar transport 122 (Ift122), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu37693	XM_006506798.2 Latest version!	Mus musculus intraflagellar transport 122 (Ift122), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu37694	XM_006506799.3	Mus musculus intraflagellar transport 122 (Ift122), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ift122 ( XM_006506799.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506862.1 Mus musculus intraflagellar transport 122 (Ift122), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu37694
Clone ID Related Accession (Same CDS sequence)	XM_006506799.4 , XM_006506799.3
Accession Version	XM_006506799.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	intraflagellar transport protein 122 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506799.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAATG GCATCATCAG TATACGGAAC AAGAATGGTG AAGAGAAAGT GAAGATCGAG 
CGCCCTGGGG GCTCCCTCTC CCCAATATGG TCCATCTGCT GGAACCCTTC AAGCCGATGG 
GAGACTATCT GGATGAGCAG AGAGAACGAG GATGCTGAGG ATGTCATTGT CAACAAATAT 
GTTCAGGAAA TCCCTTCCAT TCTGAAGTCA ACAGTGTACA ATAGTCAGGG TAGTGAGGCA 
GAGGAGGAAG AGCAAGAGGA AGAGGACGAC AGTCCCAGGG ACGGCAACTC AGAGGAACAT 
AATGACATCC TGGCTGTAGC TGACTGGGGA CAGAAACTTT CCTTCTACCA GCTGAGTGGA 
AAACAGATTG GGAAGGATCG GCCACTGAAC TTTGACCCCT GTTGCATCAG CTACTTCACT 
AAAGGAGAGT ACATTTTGGT GGGAGGTTCA GACAAGCAGG TATCTCTTTT CACCAAGGAT 
GGAGTGCGAC TGGGGACCGT TGGGGAGCAG AACTCCTGGG TGTGGACCTG TAGAGTGAAA 
CCTGACTCCA ATTATGTGGT GGTCGGCTGC CAGGATGGCA CCATCTCCTT CTACCAGCTC 
ATCTTCAGCA CAGTCCATGG GCTCTACAAA GATCGGTATG CCTACAGGGA CAGCATGACA 
GACGTCATCG TGCAGCACCT CATCACAGAG CAGAAAGTTA GGATTAAGTG CAGAGAGCTT 
GTCAAGAAAA TTGCCATCTA CAAAAATCGA TTAGCTATCC AGCTGCCAGA GAAGATCCTC 
ATCTACGAGC TGTACTCTGA AGACTCGACA GACATGCACT ACCGGGTAAA GGAGAAAATT 
GTCAAGAAGT TTGAGTGCAA CCTGCTGGTG GTGTGTGCTG ACCACATCAT CCTGTGCCAG 
GAGAAACGGC TACAGTGCCT GTCCTTCAGT GGAGTGAAGG AAAGGGAATG GCAGATGGAA 
TCTCTCATCC GCTACATCAA GGTGATCGGT GGCCCTGCTG GAAGAGAAGG TCTGTTGGTA 
GGCCTGAAAA ACGGCCAGAT CCTGAAGATC TTTGTGGACA ATCTTTTTGC TATTGTCCTG 
CTGAAGCAGG CCACAGCCGT GCGCTGCCTG GACATGAGTG CCTCCCGGAA CAAGCTGGCT 
GTGGTGGATG AGAATGACAC GTGCCTGGTG TACGACATCC ACACGAAGGA ACTGCTTTTC 
CAGGAACCGA ATGCTAACAG CGTGGCCTGG AACACGCAGT GTGAGGACAT GCTCTGCTTC 
TCTGGAGGAG GCTACCTCAA CATCAAGGCC AGCACCTTCC CAGTGCACCA GCAGAAGCTG 
CAGGGCTTTG TGGTTGGCTA CAACGGCTCC AAGATCTTCT GCCTTCATGT CTTCTCTATG 
TCTGCCGTGG AGGTGCCACA GTCCGCTCCA ATGTACCAGT ACCTCGATAG GAAGATGTTC 
AAAGAAGCAT ATCAGATCGC TTGCTTGGGA GTGACAGACG CTGATTGGCG TGAACTGGCC 
ATGGAAGCTC TAGAAGGACT AGAGTTTGAG ACGGCAAGGA AGGCCTTCAC CAGAGTCCAG 
GACCTTCGAT ACCTAGAACT GATCAGCAGT ATTGAGGAGA GGAAGAAGAG GGGAGAGACC 
AACAATGACC TGTTTCTGGC AGATGTGTTT TCCTACCAGG GGAAGTTTCA TGAAGCCGCC 
AAACTATACA AGAGGAGCGG GCATGAGAAC CTCGCTCTCG ACATGTATAC TGACCTCTGC 
ATGTTTGAGT ACGCCAAGGA TTTCCTTGGA TCTGGAGACC CTAAAGAAAC GAAGATGCTC 
ATCACCAAGC AGGCCGACTG GGCCAGAAAC ATCAATGAGC CCAAAGCTGC AGTGGAGATG 
TACATCTCAG CAGGGGAGCA CGCCAAGGCC ATAGAGATCA GCGGCAGCCA TGGCTGGGTG 
GACATGTTGA TAGACATTGC TCGGAAGCTG GACAAGGCTG AACGGGAGCC CCTGCTCATG 
TGTGCTTGCT ACTTTAAGAA GCTGGACAGT CCTGGCTATG CTGCTGAGAC CTACCTGAAG 
ATCGGGGACC TGAAGTCTCT GGTGCAGCTG TATGTGGACA CCAAGCGCTG GGATGAGGCC 
TTTGCGTTAG GTGAGAAGCA CCCGGAGTTC AAGGATGACG TCTACGTGCC CTACGCGCAG 
TGGCTGGCGG AGAACGACCG CTTTGAGGAA GCCCAGAAAG CCTTCCACAA GGCCGGGAGG 
CAGGGAGAAG CAGTCCGGGT GCTGGAGCAG CTCACGCACA ATGCTGTGGT GGAGAGCAGG 
TTTAACGACG CTGCCTATTA TTACTGGATG CTGTCCATGC AGTGCCTCGA CATGGCTCAA 
GCAGATCCTG CCCAGAAGGA CGCGATGCTG GACAAGTTTC ACCACTTCCA ACACCTGGCT 
GAGCTGTACC ATGGCTATCA AACCATTCAC CGATACACGG AGGAGCCATT CAGCTTTGAC 
CTCCCTGAAA CCCTCTTCAA CATCTCCAAG TTCTTGCTGC ACAGCCTGAC CAAGGCCACC 
CCCTTGGGTA TCTCCAAAGT GAATACACTC TTCACACTGG CTAAGCAGAG CAAGGCTCTG 
GGTGCTTACA AGCTGGCCCG GCATGCCTAT GACAAGCTAC GGGGGCTGCA GATCCCCGCC 
AGGATCCAGA AGTCCATTGA GCTGGGCACC CTGACCATCC GCTCCAAGCC CTTCCATGAC 
AGTGAGGAGC TGGTACCCTT GTGCTACCGC TGTTCTACCA ACAACCCGCT GCTGAACAAC 
CTTGGCAACG TCTGCATCAA CTGCCGCCAG CCCTTCATCT TCTCCGCCTC TTCCTATGAG 
GTGCTGCACC TGGTGGAATT CTACCTGGAA GAGGGGATCA CTGACGAAGA AGCTGTTGCC 
CTCATCGATC TGGAGGCACC AAGACACAAG AGGGAGGGCA AGTGGCGAGA GACCTCAAGC 
AACAATTCCC AGACGTTGAA GCTGGACGAG ACCATGGACT CCATAGGAGA AGATGACCCA 
TTTACAGCCA AGCTGAGCTT TGAGCAAGGT AGCTCTGAGT TTGTGCCTGT GGTGGTGAAC 
CGGTCAGTAC TACGCTCCAT GAGCCGAAGA GATGTTCTCA TCAAGCGCTG GCCCCCGCCG 
CTGCAATGGC AATATTTCCG CTCGCTGTTG CCTGATGCCT CCATCACCAT GTGCCCCTCC 
TGCTTTCAGA TGTTCCATTC AGAGGACTAC GAACTGCTTG TGCTCCAGCA CGCCTGCTGC 
CCTTACTGCC GAAGGCGTAT CGACGACACT GGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506862.1
CDS	533..3811
Translation	MANGIISIRNKNGEEKVKIERPGGSLSPIWSICWNPSSRWETIWMSRENEDAEDVIVNKYVQEIPSILKSTVYNSQGSEAEEEEQEEEDDSPRDGNSEEHNDILAVADWGQKLSFYQLSGKQIGKDRPLNFDPCCISYFTKGEYILVGGSDKQVSLFTKDGVRLGTVGEQNSWVWTCRVKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCRELVKKIAIYKNRLAIQLPEKILIYELYSEDSTDMHYRVKEKIVKKFECNLLVVCADHIILCQEKRLQCLSFSGVKEREWQMESLIRYIKVIGGPAGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRNKLAVVDENDTCLVYDIHTKELLFQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHQQKLQGFVVGYNGSKIFCLHVFSMSAVEVPQSAPMYQYLDRKMFKEAYQIACLGVTDADWRELAMEALEGLEFETARKAFTRVQDLRYLELISSIEERKKRGETNNDLFLADVFSYQGKFHEAAKLYKRSGHENLALDMYTDLCMFEYAKDFLGSGDPKETKMLITKQADWARNINEPKAAVEMYISAGEHAKAIEISGSHGWVDMLIDIARKLDKAEREPLLMCACYFKKLDSPGYAAETYLKIGDLKSLVQLYVDTKRWDEAFALGEKHPEFKDDVYVPYAQWLAENDRFEEAQKAFHKAGRQGEAVRVLEQLTHNAVVESRFNDAAYYYWMLSMQCLDMAQADPAQKDAMLDKFHHFQHLAELYHGYQTIHRYTEEPFSFDLPETLFNISKFLLHSLTKATPLGISKVNTLFTLAKQSKALGAYKLARHAYDKLRGLQIPARIQKSIELGTLTIRSKPFHDSEELVPLCYRCSTNNPLLNNLGNVCINCRQPFIFSASSYEVLHLVEFYLEEGITDEEAVALIDLEAPRHKREGKWRETSSNNSQTLKLDETMDSIGEDDPFTAKLSFEQGSSEFVPVVVNRSVLRSMSRRDVLIKRWPPPLQWQYFRSLLPDASITMCPSCFQMFHSEDYELLVLQHACCPYCRRRIDDTGP

Target ORF information:

RefSeq Version	XM_006506799.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus intraflagellar transport 122 (Ift122), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506799.4

ORF Insert Sequence:

ATGGCCAATG GCATCATCAG TATACGGAAC AAGAATGGTG AAGAGAAAGT GAAGATCGAG 
CGCCCTGGGG GCTCCCTCTC CCCAATATGG TCCATCTGCT GGAACCCTTC AAGCCGATGG 
GAGACTATCT GGATGAGCAG AGAGAACGAG GATGCTGAGG ATGTCATTGT CAACAAATAT 
GTTCAGGAAA TCCCTTCCAT TCTGAAGTCA ACAGTGTACA ATAGTCAGGG TAGTGAGGCA 
GAGGAGGAAG AGCAAGAGGA AGAGGACGAC AGTCCCAGGG ACGGCAACTC AGAGGAACAT 
AATGACATCC TGGCTGTAGC TGACTGGGGA CAGAAACTTT CCTTCTACCA GCTGAGTGGA 
AAACAGATTG GGAAGGATCG GCCACTGAAC TTTGACCCCT GTTGCATCAG CTACTTCACT 
AAAGGAGAGT ACATTTTGGT GGGAGGTTCA GACAAGCAGG TATCTCTTTT CACCAAGGAT 
GGAGTGCGAC TGGGGACCGT TGGGGAGCAG AACTCCTGGG TGTGGACCTG TAGAGTGAAA 
CCTGACTCCA ATTATGTGGT GGTCGGCTGC CAGGATGGCA CCATCTCCTT CTACCAGCTC 
ATCTTCAGCA CAGTCCATGG GCTCTACAAA GATCGGTATG CCTACAGGGA CAGCATGACA 
GACGTCATCG TGCAGCACCT CATCACAGAG CAGAAAGTTA GGATTAAGTG CAGAGAGCTT 
GTCAAGAAAA TTGCCATCTA CAAAAATCGA TTAGCTATCC AGCTGCCAGA GAAGATCCTC 
ATCTACGAGC TGTACTCTGA AGACTCGACA GACATGCACT ACCGGGTAAA GGAGAAAATT 
GTCAAGAAGT TTGAGTGCAA CCTGCTGGTG GTGTGTGCTG ACCACATCAT CCTGTGCCAG 
GAGAAACGGC TACAGTGCCT GTCCTTCAGT GGAGTGAAGG AAAGGGAATG GCAGATGGAA 
TCTCTCATCC GCTACATCAA GGTGATCGGT GGCCCTGCTG GAAGAGAAGG TCTGTTGGTA 
GGCCTGAAAA ACGGCCAGAT CCTGAAGATC TTTGTGGACA ATCTTTTTGC TATTGTCCTG 
CTGAAGCAGG CCACAGCCGT GCGCTGCCTG GACATGAGTG CCTCCCGGAA CAAGCTGGCT 
GTGGTGGATG AGAATGACAC GTGCCTGGTG TACGACATCC ACACGAAGGA ACTGCTTTTC 
CAGGAACCGA ATGCTAACAG CGTGGCCTGG AACACGCAGT GTGAGGACAT GCTCTGCTTC 
TCTGGAGGAG GCTACCTCAA CATCAAGGCC AGCACCTTCC CAGTGCACCA GCAGAAGCTG 
CAGGGCTTTG TGGTTGGCTA CAACGGCTCC AAGATCTTCT GCCTTCATGT CTTCTCTATG 
TCTGCCGTGG AGGTGCCACA GTCCGCTCCA ATGTACCAGT ACCTCGATAG GAAGATGTTC 
AAAGAAGCAT ATCAGATCGC TTGCTTGGGA GTGACAGACG CTGATTGGCG TGAACTGGCC 
ATGGAAGCTC TAGAAGGACT AGAGTTTGAG ACGGCAAGGA AGGCCTTCAC CAGAGTCCAG 
GACCTTCGAT ACCTAGAACT GATCAGCAGT ATTGAGGAGA GGAAGAAGAG GGGAGAGACC 
AACAATGACC TGTTTCTGGC AGATGTGTTT TCCTACCAGG GGAAGTTTCA TGAAGCCGCC 
AAACTATACA AGAGGAGCGG GCATGAGAAC CTCGCTCTCG ACATGTATAC TGACCTCTGC 
ATGTTTGAGT ACGCCAAGGA TTTCCTTGGA TCTGGAGACC CTAAAGAAAC GAAGATGCTC 
ATCACCAAGC AGGCCGACTG GGCCAGAAAC ATCAATGAGC CCAAAGCTGC AGTGGAGATG 
TACATCTCAG CAGGGGAGCA CGCCAAGGCC ATAGAGATCA GCGGCAGCCA TGGCTGGGTG 
GACATGTTGA TAGACATTGC TCGGAAGCTG GACAAGGCTG AACGGGAGCC CCTGCTCATG 
TGTGCTTGCT ACTTTAAGAA GCTGGACAGT CCTGGCTATG CTGCTGAGAC CTACCTGAAG 
ATCGGGGACC TGAAGTCTCT GGTGCAGCTG TATGTGGACA CCAAGCGCTG GGATGAGGCC 
TTTGCGTTAG GTGAGAAGCA CCCGGAGTTC AAGGATGACG TCTACGTGCC CTACGCGCAG 
TGGCTGGCGG AGAACGACCG CTTTGAGGAA GCCCAGAAAG CCTTCCACAA GGCCGGGAGG 
CAGGGAGAAG CAGTCCGGGT GCTGGAGCAG CTCACGCACA ATGCTGTGGT GGAGAGCAGG 
TTTAACGACG CTGCCTATTA TTACTGGATG CTGTCCATGC AGTGCCTCGA CATGGCTCAA 
GCAGATCCTG CCCAGAAGGA CGCGATGCTG GACAAGTTTC ACCACTTCCA ACACCTGGCT 
GAGCTGTACC ATGGCTATCA AACCATTCAC CGATACACGG AGGAGCCATT CAGCTTTGAC 
CTCCCTGAAA CCCTCTTCAA CATCTCCAAG TTCTTGCTGC ACAGCCTGAC CAAGGCCACC 
CCCTTGGGTA TCTCCAAAGT GAATACACTC TTCACACTGG CTAAGCAGAG CAAGGCTCTG 
GGTGCTTACA AGCTGGCCCG GCATGCCTAT GACAAGCTAC GGGGGCTGCA GATCCCCGCC 
AGGATCCAGA AGTCCATTGA GCTGGGCACC CTGACCATCC GCTCCAAGCC CTTCCATGAC 
AGTGAGGAGC TGGTACCCTT GTGCTACCGC TGTTCTACCA ACAACCCGCT GCTGAACAAC 
CTTGGCAACG TCTGCATCAA CTGCCGCCAG CCCTTCATCT TCTCCGCCTC TTCCTATGAG 
GTGCTGCACC TGGTGGAATT CTACCTGGAA GAGGGGATCA CTGACGAAGA AGCTGTTGCC 
CTCATCGATC TGGAGGCACC AAGACACAAG AGGGAGGGCA AGTGGCGAGA GACCTCAAGC 
AACAATTCCC AGACGTTGAA GCTGGACGAG ACCATGGACT CCATAGGAGA AGATGACCCA 
TTTACAGCCA AGCTGAGCTT TGAGCAAGGT AGCTCTGAGT TTGTGCCTGT GGTGGTGAAC 
CGGTCAGTAC TACGCTCCAT GAGCCGAAGA GATGTTCTCA TCAAGCGCTG GCCCCCGCCG 
CTGCAATGGC AATATTTCCG CTCGCTGTTG CCTGATGCCT CCATCACCAT GTGCCCCTCC 
TGCTTTCAGA TGTTCCATTC AGAGGACTAC GAACTGCTTG TGCTCCAGCA CGCCTGCTGC 
CCTTACTGCC GAAGGCGTAT CGACGACACT GGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ift122 ( NM_031177.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_112454.2 Mus musculus intraflagellar transport 122 (Ift122), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu08494
Clone ID Related Accession (Same CDS sequence)	NM_031177.4
Accession Version	NM_031177.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	intraflagellar transport protein 122 homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY085672.1, AK167412.1, AK044456.1 and CO043683.1. On Nov 17, 2009 this sequence version replaced NM_031177.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK044456.1, SRR1660811.129605.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGGCCG TGTTGACGTG GAGAGACAAA GCGGAGCAGT GTATCTACGA CCTCGCATTT 
AAGCCTGATG GAACACAACT GATTCTGGCT GCAGGAAACA GATTGCTGGT TTATGACACC 
TCTGATGGCA CCTTACTTCA GCCCCTGAAG GGACACAAAG ACACCGTGTA CTGTGTGGCG 
TATGCAAAGG ATGGCAAGCG CTTTGCATCT GGATCTGCTG ACAAGAGCAT CATTATCTGG 
ACCTCAAAAC TTGAAGGCAT TCTGAAGTAC ACGCACAACG ACTCTATACA GTGTGTCTCC 
TACAATCCTG TTACCCACCA GCTGGCATCT TGTTCCTCCA GTGACTTTGG GTTATGGTCT 
CCTGAGCAGA AGTCTGTGTC CAAGCACAAA TCCAGCAGCA AGATCACGTG CTGCAGCTGG 
ACAAACGATG GTCAGTACCT GGCTCTGGGC ATGGCCAATG GCATCATCAG TATACGGAAC 
AAGAATGGTG AAGAGAAAGT GAAGATCGAG CGCCCTGGGG GCTCCCTCTC CCCAATATGG 
TCCATCTGCT GGAACCCTTC AAGAGAGGAA CATAATGACA TCCTGGCTGT AGCTGACTGG 
GGACAGAAAC TTTCCTTCTA CCAGCTGAGT GGAAAACAGA TTGGGAAGGA TCGGCCACTG 
AACTTTGACC CCTGTTGCAT CAGCTACTTC ACTAAAGGAG AGTACATTTT GGTGGGAGGT 
TCAGACAAGC AGGTATCTCT TTTCACCAAG GATGGAGTGC GACTGGGGAC CGTTGGGGAG 
CAGAACTCCT GGGTGTGGAC CTGTAGAGTG AAACCTGACT CCAATTATGT GGTGGTCGGC 
TGCCAGGATG GCACCATCTC CTTCTACCAG CTCATCTTCA GCACAGTCCA TGGGCTCTAC 
AAAGATCGGT ATGCCTACAG GGACAGCATG ACAGACGTCA TCGTGCAGCA CCTCATCACA 
GAGCAGAAAG TTAGGATTAA GTGCAGAGAG CTTGTCAAGA AAATTGCCAT CTACAAAAAT 
CGATTAGCTA TCCAGCTGCC AGAGAAGATC CTCATCTACG AGCTGTACTC TGAAGACTCG 
ACAGACATGC ACTACCGGGT AAAGGAGAAA ATTGTCAAGA AGTTTGAGTG CAACCTGCTG 
GTGGTGTGTG CTGACCACAT CATCCTGTGC CAGGAGAAAC GGCTACAGTG CCTGTCCTTC 
AGTGGAGTGA AGGAAAGGGA ATGGCAGATG GAATCTCTCA TCCGCTACAT CAAGGTGATC 
GGTGGCCCTG CTGGAAGAGA AGGTCTGTTG GTAGGCCTGA AAAACGGCCA GATCCTGAAG 
ATCTTTGTGG ACAATCTTTT TGCTATTGTC CTGCTGAAGC AGGCCACAGC CGTGCGCTGC 
CTGGACATGA GTGCCTCCCG GAACAAGCTG GCTGTGGTGG ATGAGAATGA CACGTGCCTG 
GTGTACGACA TCCACACGAA GGAACTGCTT TTCCAGGAAC CGAATGCTAA CAGCGTGGCC 
TGGAACACGC AGTGTGAGGA CATGCTCTGC TTCTCTGGAG GAGGCTACCT CAACATCAAG 
GCCAGCACCT TCCCAGTGCA CCAGCAGAAG CTGCAGGGCT TTGTGGTTGG CTACAACGGC 
TCCAAGATCT TCTGCCTTCA TGTCTTCTCT ATGTCTGCCG TGGAGGTGCC ACAGTCCGCT 
CCAATGTACC AGTACCTCGA TAGGAAGATG TTCAAAGAAG CATATCAGAT CGCTTGCTTG 
GGAGTGACAG ACGCTGATTG GCGTGAACTG GCCATGGAAG CTCTAGAAGG ACTAGAGTTT 
GAGACGGCAA GGAAGGCCTT CACCAGAGTC CAGGACCTTC GATACCTAGA ACTGATCAGC 
AGTATTGAGG AGAGGAAGAA GAGGGGAGAG ACCAACAATG ACCTGTTTCT GGCAGATGTG 
TTTTCCTACC AGGGGAAGTT TCATGAAGCC GCCAAACTAT ACAAGAGGAG CGGGCATGAG 
AACCTCGCTC TCGACATGTA TACTGACCTC TGCATGTTTG AGTACGCCAA GGATTTCCTT 
GGATCTGGAG ACCCTAAAGA AACGAAGATG CTCATCACCA AGCAGGCCGA CTGGGCCAGA 
AACATCAATG AGCCCAAAGC TGCAGTGGAG ATGTACATCT CAGCAGGGGA GCACGCCAAG 
GCCATAGAGA TCAGCGGCAG CCATGGCTGG GTGGACATGT TGATAGACAT TGCTCGGAAG 
CTGGACAAGG CTGAACGGGA GCCCCTGCTC ATGTGTGCTT GCTACTTTAA GAAGCTGGAC 
AGTCCTGGCT ATGCTGCTGA GACCTACCTG AAGATCGGGG ACCTGAAGTC TCTGGTGCAG 
CTGTATGTGG ACACCAAGCG CTGGGATGAG GCCTTTGCGT TAGGTGAGAA GCACCCGGAG 
TTCAAGGATG ACGTCTACGT GCCCTACGCG CAGTGGCTGG CGGAGAACGA CCGCTTTGAG 
GAAGCCCAGA AAGCCTTCCA CAAGGCCGGG AGGCAGGGAG AAGCAGTCCG GGTGCTGGAG 
CAGCTCACGC ACAATGCTGT GGTGGAGAGC AGGTTTAACG ACGCTGCCTA TTATTACTGG 
ATGCTGTCCA TGCAGTGCCT CGACATGGCT CAAGCAGATC CTGCCCAGAA GGACGCGATG 
CTGGACAAGT TTCACCACTT CCAACACCTG GCTGAGCTGT ACCATGGCTA TCAAACCATT 
CACCGATACA CGGAGGAGCC ATTCAGCTTT GACCTCCCTG AAACCCTCTT CAACATCTCC 
AAGTTCTTGC TGCACAGCCT GACCAAGGCC ACCCCCTTGG GTATCTCCAA AGTGAATACA 
CTCTTCACAC TGGCTAAGCA GAGCAAGGCT CTGGGTGCTT ACAAGCTGGC CCGGCATGCC 
TATGACAAGC TACGGGGGCT GCAGATCCCC GCCAGGATCC AGAAGTCCAT TGAGCTGGGC 
ACCCTGACCA TCCGCTCCAA GCCCTTCCAT GACAGTGAGG AGCTGGTACC CTTGTGCTAC 
CGCTGTTCTA CCAACAACCC GCTGCTGAAC AACCTTGGCA ACGTCTGCAT CAACTGCCGC 
CAGCCCTTCA TCTTCTCCGC CTCTTCCTAT GAGGTGCTGC ACCTGGTGGA ATTCTACCTG 
GAAGAGGGGA TCACTGACGA AGAAGCTGTT GCCCTCATCG ATCTGGAGGC ACCAAGACAC 
AAGAGGGAGG GCAAGTGGCG AGAGACCTCA AGCAACAATT CCCAGACGTT GAAGCTGGAC 
GAGACCATGG ACTCCATAGG AGAAGATGAC CCATTTACAG CCAAGCTGAG CTTTGAGCAA 
GGTAGCTCTG AGTTTGTGCC TGTGGTGGTG AACCGGTCAG TACTACGCTC CATGAGCCGA 
AGAGATGTTC TCATCAAGCG CTGGCCCCCG CCGCTGCAAT GGCAATATTT CCGCTCGCTG 
TTGCCTGATG CCTCCATCAC CATGTGCCCC TCCTGCTTTC AGATGTTCCA TTCAGAGGAC 
TACGAACTGC TTGTGCTCCA GCACGCCTGC TGCCCTTACT GCCGAAGGCG TATCGACGAC 
ACTGGCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112454.2
CDS	142..3693
Translation	MRAVLTWRDKAEQCIYDLAFKPDGTQLILAAGNRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGKRFASGSADKSIIIWTSKLEGILKYTHNDSIQCVSYNPVTHQLASCSSSDFGLWSPEQKSVSKHKSSSKITCCSWTNDGQYLALGMANGIISIRNKNGEEKVKIERPGGSLSPIWSICWNPSREEHNDILAVADWGQKLSFYQLSGKQIGKDRPLNFDPCCISYFTKGEYILVGGSDKQVSLFTKDGVRLGTVGEQNSWVWTCRVKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCRELVKKIAIYKNRLAIQLPEKILIYELYSEDSTDMHYRVKEKIVKKFECNLLVVCADHIILCQEKRLQCLSFSGVKEREWQMESLIRYIKVIGGPAGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRNKLAVVDENDTCLVYDIHTKELLFQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHQQKLQGFVVGYNGSKIFCLHVFSMSAVEVPQSAPMYQYLDRKMFKEAYQIACLGVTDADWRELAMEALEGLEFETARKAFTRVQDLRYLELISSIEERKKRGETNNDLFLADVFSYQGKFHEAAKLYKRSGHENLALDMYTDLCMFEYAKDFLGSGDPKETKMLITKQADWARNINEPKAAVEMYISAGEHAKAIEISGSHGWVDMLIDIARKLDKAEREPLLMCACYFKKLDSPGYAAETYLKIGDLKSLVQLYVDTKRWDEAFALGEKHPEFKDDVYVPYAQWLAENDRFEEAQKAFHKAGRQGEAVRVLEQLTHNAVVESRFNDAAYYYWMLSMQCLDMAQADPAQKDAMLDKFHHFQHLAELYHGYQTIHRYTEEPFSFDLPETLFNISKFLLHSLTKATPLGISKVNTLFTLAKQSKALGAYKLARHAYDKLRGLQIPARIQKSIELGTLTIRSKPFHDSEELVPLCYRCSTNNPLLNNLGNVCINCRQPFIFSASSYEVLHLVEFYLEEGITDEEAVALIDLEAPRHKREGKWRETSSNNSQTLKLDETMDSIGEDDPFTAKLSFEQGSSEFVPVVVNRSVLRSMSRRDVLIKRWPPPLQWQYFRSLLPDASITMCPSCFQMFHSEDYELLVLQHACCPYCRRRIDDTGP

Target ORF information:

RefSeq Version	NM_031177.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus intraflagellar transport 122 (Ift122), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031177.4

ORF Insert Sequence:

ATGAGGGCCG TGTTGACGTG GAGAGACAAA GCGGAGCAGT GTATCTACGA CCTCGCATTT 
AAGCCTGATG GAACACAACT GATTCTGGCT GCAGGAAACA GATTGCTGGT TTATGACACC 
TCTGATGGCA CCTTACTTCA GCCCCTGAAG GGACACAAAG ACACCGTGTA CTGTGTGGCG 
TATGCAAAGG ATGGCAAGCG CTTTGCATCT GGATCTGCTG ACAAGAGCAT CATTATCTGG 
ACCTCAAAAC TTGAAGGCAT TCTGAAGTAC ACGCACAACG ACTCTATACA GTGTGTCTCC 
TACAATCCTG TTACCCACCA GCTGGCATCT TGTTCCTCCA GTGACTTTGG GTTATGGTCT 
CCTGAGCAGA AGTCTGTGTC CAAGCACAAA TCCAGCAGCA AGATCACGTG CTGCAGCTGG 
ACAAACGATG GTCAGTACCT GGCTCTGGGC ATGGCCAATG GCATCATCAG TATACGGAAC 
AAGAATGGTG AAGAGAAAGT GAAGATCGAG CGCCCTGGGG GCTCCCTCTC CCCAATATGG 
TCCATCTGCT GGAACCCTTC AAGAGAGGAA CATAATGACA TCCTGGCTGT AGCTGACTGG 
GGACAGAAAC TTTCCTTCTA CCAGCTGAGT GGAAAACAGA TTGGGAAGGA TCGGCCACTG 
AACTTTGACC CCTGTTGCAT CAGCTACTTC ACTAAAGGAG AGTACATTTT GGTGGGAGGT 
TCAGACAAGC AGGTATCTCT TTTCACCAAG GATGGAGTGC GACTGGGGAC CGTTGGGGAG 
CAGAACTCCT GGGTGTGGAC CTGTAGAGTG AAACCTGACT CCAATTATGT GGTGGTCGGC 
TGCCAGGATG GCACCATCTC CTTCTACCAG CTCATCTTCA GCACAGTCCA TGGGCTCTAC 
AAAGATCGGT ATGCCTACAG GGACAGCATG ACAGACGTCA TCGTGCAGCA CCTCATCACA 
GAGCAGAAAG TTAGGATTAA GTGCAGAGAG CTTGTCAAGA AAATTGCCAT CTACAAAAAT 
CGATTAGCTA TCCAGCTGCC AGAGAAGATC CTCATCTACG AGCTGTACTC TGAAGACTCG 
ACAGACATGC ACTACCGGGT AAAGGAGAAA ATTGTCAAGA AGTTTGAGTG CAACCTGCTG 
GTGGTGTGTG CTGACCACAT CATCCTGTGC CAGGAGAAAC GGCTACAGTG CCTGTCCTTC 
AGTGGAGTGA AGGAAAGGGA ATGGCAGATG GAATCTCTCA TCCGCTACAT CAAGGTGATC 
GGTGGCCCTG CTGGAAGAGA AGGTCTGTTG GTAGGCCTGA AAAACGGCCA GATCCTGAAG 
ATCTTTGTGG ACAATCTTTT TGCTATTGTC CTGCTGAAGC AGGCCACAGC CGTGCGCTGC 
CTGGACATGA GTGCCTCCCG GAACAAGCTG GCTGTGGTGG ATGAGAATGA CACGTGCCTG 
GTGTACGACA TCCACACGAA GGAACTGCTT TTCCAGGAAC CGAATGCTAA CAGCGTGGCC 
TGGAACACGC AGTGTGAGGA CATGCTCTGC TTCTCTGGAG GAGGCTACCT CAACATCAAG 
GCCAGCACCT TCCCAGTGCA CCAGCAGAAG CTGCAGGGCT TTGTGGTTGG CTACAACGGC 
TCCAAGATCT TCTGCCTTCA TGTCTTCTCT ATGTCTGCCG TGGAGGTGCC ACAGTCCGCT 
CCAATGTACC AGTACCTCGA TAGGAAGATG TTCAAAGAAG CATATCAGAT CGCTTGCTTG 
GGAGTGACAG ACGCTGATTG GCGTGAACTG GCCATGGAAG CTCTAGAAGG ACTAGAGTTT 
GAGACGGCAA GGAAGGCCTT CACCAGAGTC CAGGACCTTC GATACCTAGA ACTGATCAGC 
AGTATTGAGG AGAGGAAGAA GAGGGGAGAG ACCAACAATG ACCTGTTTCT GGCAGATGTG 
TTTTCCTACC AGGGGAAGTT TCATGAAGCC GCCAAACTAT ACAAGAGGAG CGGGCATGAG 
AACCTCGCTC TCGACATGTA TACTGACCTC TGCATGTTTG AGTACGCCAA GGATTTCCTT 
GGATCTGGAG ACCCTAAAGA AACGAAGATG CTCATCACCA AGCAGGCCGA CTGGGCCAGA 
AACATCAATG AGCCCAAAGC TGCAGTGGAG ATGTACATCT CAGCAGGGGA GCACGCCAAG 
GCCATAGAGA TCAGCGGCAG CCATGGCTGG GTGGACATGT TGATAGACAT TGCTCGGAAG 
CTGGACAAGG CTGAACGGGA GCCCCTGCTC ATGTGTGCTT GCTACTTTAA GAAGCTGGAC 
AGTCCTGGCT ATGCTGCTGA GACCTACCTG AAGATCGGGG ACCTGAAGTC TCTGGTGCAG 
CTGTATGTGG ACACCAAGCG CTGGGATGAG GCCTTTGCGT TAGGTGAGAA GCACCCGGAG 
TTCAAGGATG ACGTCTACGT GCCCTACGCG CAGTGGCTGG CGGAGAACGA CCGCTTTGAG 
GAAGCCCAGA AAGCCTTCCA CAAGGCCGGG AGGCAGGGAG AAGCAGTCCG GGTGCTGGAG 
CAGCTCACGC ACAATGCTGT GGTGGAGAGC AGGTTTAACG ACGCTGCCTA TTATTACTGG 
ATGCTGTCCA TGCAGTGCCT CGACATGGCT CAAGCAGATC CTGCCCAGAA GGACGCGATG 
CTGGACAAGT TTCACCACTT CCAACACCTG GCTGAGCTGT ACCATGGCTA TCAAACCATT 
CACCGATACA CGGAGGAGCC ATTCAGCTTT GACCTCCCTG AAACCCTCTT CAACATCTCC 
AAGTTCTTGC TGCACAGCCT GACCAAGGCC ACCCCCTTGG GTATCTCCAA AGTGAATACA 
CTCTTCACAC TGGCTAAGCA GAGCAAGGCT CTGGGTGCTT ACAAGCTGGC CCGGCATGCC 
TATGACAAGC TACGGGGGCT GCAGATCCCC GCCAGGATCC AGAAGTCCAT TGAGCTGGGC 
ACCCTGACCA TCCGCTCCAA GCCCTTCCAT GACAGTGAGG AGCTGGTACC CTTGTGCTAC 
CGCTGTTCTA CCAACAACCC GCTGCTGAAC AACCTTGGCA ACGTCTGCAT CAACTGCCGC 
CAGCCCTTCA TCTTCTCCGC CTCTTCCTAT GAGGTGCTGC ACCTGGTGGA ATTCTACCTG 
GAAGAGGGGA TCACTGACGA AGAAGCTGTT GCCCTCATCG ATCTGGAGGC ACCAAGACAC 
AAGAGGGAGG GCAAGTGGCG AGAGACCTCA AGCAACAATT CCCAGACGTT GAAGCTGGAC 
GAGACCATGG ACTCCATAGG AGAAGATGAC CCATTTACAG CCAAGCTGAG CTTTGAGCAA 
GGTAGCTCTG AGTTTGTGCC TGTGGTGGTG AACCGGTCAG TACTACGCTC CATGAGCCGA 
AGAGATGTTC TCATCAAGCG CTGGCCCCCG CCGCTGCAAT GGCAATATTT CCGCTCGCTG 
TTGCCTGATG CCTCCATCAC CATGTGCCCC TCCTGCTTTC AGATGTTCCA TTCAGAGGAC 
TACGAACTGC TTGTGCTCCA GCACGCCTGC TGCCCTTACT GCCGAAGGCG TATCGACGAC 
ACTGGCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ift122 ( NM_001167763.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001161235.1 Mus musculus intraflagellar transport 122 (Ift122), transcript variant 2, mRNA.

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CloneID	OMu11119
Clone ID Related Accession (Same CDS sequence)	NM_001167763.1
Accession Version	NM_001167763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	intraflagellar transport protein 122 homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY085672.1, AK167412.1 and CO043683.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is one aa shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC066083.1, AK131115.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGGCCG TGTTGACGTG GAGAGACAAA GCGGAGCAGT GTATCTACGA CCTCGCATTT 
AAGCCTGATG GAACACAACT GATTCTGGCT GCAGGAAACA GATTGCTGGT TTATGACACC 
TCTGATGGCA CCTTACTTCA GCCCCTGAAG GGACACAAAG ACACCGTGTA CTGTGTGGCG 
TATGCAAAGG ATGGCAAGCG CTTTGCATCT GGATCTGCTG ACAAGAGCAT CATTATCTGG 
ACCTCAAAAC TTGAAGGCAT TCTGAAGTAC ACGCACAACG ACTCTATACA GTGTGTCTCC 
TACAATCCTG TTACCCACCA GCTGGCATCT TGTTCCTCCA GTGACTTTGG GTTATGGTCT 
CCTGAGCAGA AGTCTGTGTC CAAGCACAAA TCCAGCAGCA AGATCACGTG CTGCAGCTGG 
ACAAACGATG GTCAGTACCT GGCTCTGGGC ATGGCCAATG GCATCATCAG TATACGGAAC 
AAGAATGGTG AAGAGAAAGT GAAGATCGAG CGCCCTGGGG GCTCCCTCTC CCCAATATGG 
TCCATCTGCT GGAACCCTTC AAGAGAGGAA CATAATGACA TCCTGGCTGT AGCTGACTGG 
GGACAGAAAC TTTCCTTCTA CCAGCTGAGT GGAAAACAGA TTGGGAAGGA TCGGCCACTG 
AACTTTGACC CCTGTTGCAT CAGCTACTTC ACTAAAGGAG AGTACATTTT GGTGGGAGGT 
TCAGACAAGC AGGTATCTCT TTTCACCAAG GATGGAGTGC GACTGGGGAC CGTTGGGGAG 
CAGAACTCCT GGGTGTGGAC CTGTAGAGTG AAACCTGACT CCAATTATGT GGTGGTCGGC 
TGCCAGGATG GCACCATCTC CTTCTACCAG CTCATCTTCA GCACAGTCCA TGGGCTCTAC 
AAAGATCGGT ATGCCTACAG GGACAGCATG ACAGACGTCA TCGTGCAGCA CCTCATCACA 
GAGCAGAAAG TTAGGATTAA GTGCAGAGAG CTTGTCAAGA AAATTGCCAT CTACAAAAAT 
CGATTAGCTA TCCAGCTGCC AGAGAAGATC CTCATCTACG AGCTGTACTC TGAAGACTCG 
ACAGACATGC ACTACCGGGT AAAGGAGAAA ATTGTCAAGA AGTTTGAGTG CAACCTGCTG 
GTGGTGTGTG CTGACCACAT CATCCTGTGC CAGGAGAAAC GGCTACAGTG CCTGTCCTTC 
AGTGGAGTGA AGGAAAGGGA ATGGCAGATG GAATCTCTCA TCCGCTACAT CAAGGTGATC 
GGTGGCCCTG CTGGAAGAGA AGGTCTGTTG GTAGGCCTGA AAAACGGCCA GATCCTGAAG 
ATCTTTGTGG ACAATCTTTT TGCTATTGTC CTGCTGAAGC AGGCCACAGC CGTGCGCTGC 
CTGGACATGA GTGCCTCCCG GAACAAGCTG GCTGTGGTGG ATGAGAATGA CACGTGCCTG 
GTGTACGACA TCCACACGAA GGAACTGCTT TTCCAGGAAC CGAATGCTAA CAGCGTGGCC 
TGGAACACGC AGTGTGAGGA CATGCTCTGC TTCTCTGGAG GAGGCTACCT CAACATCAAG 
GCCAGCACCT TCCCAGTGCA CCAGCAGAAG CTGCAGGGCT TTGTGGTTGG CTACAACGGC 
TCCAAGATCT TCTGCCTTCA TGTCTTCTCT ATGTCTGCCG TGGAGGTGCC ACAGTCCGCT 
CCAATGTACC AGTACCTCGA TAGGAAGATG TTCAAAGAAG CATATCAGAT CGCTTGCTTG 
GGAGTGACAG ACGCTGATTG GCGTGAACTG GCCATGGAAG CTCTAGAAGG ACTAGAGTTT 
GAGACGGCAA GGAAGGCCTT CACCAGAGTC CAGGACCTTC GATACCTAGA ACTGATCAGC 
AGTATTGAGG AGAGGAAGAA GAGGGGAGAG ACCAACAATG ACCTGTTTCT GGCAGATGTG 
TTTTCCTACC AGGGGAAGTT TCATGAAGCC GCCAAACTAT ACAAGAGGAG CGGGCATGAG 
AACCTCGCTC TCGACATGTA TACTGACCTC TGCATGTTTG AGTACGCCAA GGATTTCCTT 
GGATCTGGAG ACCCTAAAGA AACGAAGATG CTCATCACCA AGCAGGCCGA CTGGGCCAGA 
AACATCAATG AGCCCAAAGC TGCAGTGGAG ATGTACATCT CAGCAGGGGA GCACGCCAAG 
GCCATAGAGA TCAGCGGCAG CCATGGCTGG GTGGACATGT TGATAGACAT TGCTCGGAAG 
CTGGACAAGG CTGAACGGGA GCCCCTGCTC ATGTGTGCTT GCTACTTTAA GAAGCTGGAC 
AGTCCTGGCT ATGCTGCTGA GACCTACCTG AAGATCGGGG ACCTGAAGTC TCTGGTGCAG 
CTGTATGTGG ACACCAAGCG CTGGGATGAG GCCTTTGCGT TAGGTGAGAA GCACCCGGAG 
TTCAAGGATG ACGTCTACGT GCCCTACGCG CAGTGGCTGG CGGAGAACGA CCGCTTTGAG 
GAAGCCCAGA AAGCCTTCCA CAAGGCCGGG AGGCAGGGAG AAGCAGTCCG GGTGCTGGAG 
CAGCTCACGC ACAATGCTGT GGTGGAGAGC AGGTTTAACG ACGCTGCCTA TTATTACTGG 
ATGCTGTCCA TGCAGTGCCT CGACATGGCT CAAGATCCTG CCCAGAAGGA CGCGATGCTG 
GACAAGTTTC ACCACTTCCA ACACCTGGCT GAGCTGTACC ATGGCTATCA AACCATTCAC 
CGATACACGG AGGAGCCATT CAGCTTTGAC CTCCCTGAAA CCCTCTTCAA CATCTCCAAG 
TTCTTGCTGC ACAGCCTGAC CAAGGCCACC CCCTTGGGTA TCTCCAAAGT GAATACACTC 
TTCACACTGG CTAAGCAGAG CAAGGCTCTG GGTGCTTACA AGCTGGCCCG GCATGCCTAT 
GACAAGCTAC GGGGGCTGCA GATCCCCGCC AGGATCCAGA AGTCCATTGA GCTGGGCACC 
CTGACCATCC GCTCCAAGCC CTTCCATGAC AGTGAGGAGC TGGTACCCTT GTGCTACCGC 
TGTTCTACCA ACAACCCGCT GCTGAACAAC CTTGGCAACG TCTGCATCAA CTGCCGCCAG 
CCCTTCATCT TCTCCGCCTC TTCCTATGAG GTGCTGCACC TGGTGGAATT CTACCTGGAA 
GAGGGGATCA CTGACGAAGA AGCTGTTGCC CTCATCGATC TGGAGGCACC AAGACACAAG 
AGGGAGGGCA AGTGGCGAGA GACCTCAAGC AACAATTCCC AGACGTTGAA GCTGGACGAG 
ACCATGGACT CCATAGGAGA AGATGACCCA TTTACAGCCA AGCTGAGCTT TGAGCAAGGT 
AGCTCTGAGT TTGTGCCTGT GGTGGTGAAC CGGTCAGTAC TACGCTCCAT GAGCCGAAGA 
GATGTTCTCA TCAAGCGCTG GCCCCCGCCG CTGCAATGGC AATATTTCCG CTCGCTGTTG 
CCTGATGCCT CCATCACCAT GTGCCCCTCC TGCTTTCAGA TGTTCCATTC AGAGGACTAC 
GAACTGCTTG TGCTCCAGCA CGCCTGCTGC CCTTACTGCC GAAGGCGTAT CGACGACACT 
GGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161235.1
CDS	142..3690
Translation	MRAVLTWRDKAEQCIYDLAFKPDGTQLILAAGNRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGKRFASGSADKSIIIWTSKLEGILKYTHNDSIQCVSYNPVTHQLASCSSSDFGLWSPEQKSVSKHKSSSKITCCSWTNDGQYLALGMANGIISIRNKNGEEKVKIERPGGSLSPIWSICWNPSREEHNDILAVADWGQKLSFYQLSGKQIGKDRPLNFDPCCISYFTKGEYILVGGSDKQVSLFTKDGVRLGTVGEQNSWVWTCRVKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCRELVKKIAIYKNRLAIQLPEKILIYELYSEDSTDMHYRVKEKIVKKFECNLLVVCADHIILCQEKRLQCLSFSGVKEREWQMESLIRYIKVIGGPAGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRNKLAVVDENDTCLVYDIHTKELLFQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHQQKLQGFVVGYNGSKIFCLHVFSMSAVEVPQSAPMYQYLDRKMFKEAYQIACLGVTDADWRELAMEALEGLEFETARKAFTRVQDLRYLELISSIEERKKRGETNNDLFLADVFSYQGKFHEAAKLYKRSGHENLALDMYTDLCMFEYAKDFLGSGDPKETKMLITKQADWARNINEPKAAVEMYISAGEHAKAIEISGSHGWVDMLIDIARKLDKAEREPLLMCACYFKKLDSPGYAAETYLKIGDLKSLVQLYVDTKRWDEAFALGEKHPEFKDDVYVPYAQWLAENDRFEEAQKAFHKAGRQGEAVRVLEQLTHNAVVESRFNDAAYYYWMLSMQCLDMAQDPAQKDAMLDKFHHFQHLAELYHGYQTIHRYTEEPFSFDLPETLFNISKFLLHSLTKATPLGISKVNTLFTLAKQSKALGAYKLARHAYDKLRGLQIPARIQKSIELGTLTIRSKPFHDSEELVPLCYRCSTNNPLLNNLGNVCINCRQPFIFSASSYEVLHLVEFYLEEGITDEEAVALIDLEAPRHKREGKWRETSSNNSQTLKLDETMDSIGEDDPFTAKLSFEQGSSEFVPVVVNRSVLRSMSRRDVLIKRWPPPLQWQYFRSLLPDASITMCPSCFQMFHSEDYELLVLQHACCPYCRRRIDDTGP

Target ORF information:

RefSeq Version	NM_001167763.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus intraflagellar transport 122 (Ift122), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001167763.1

ORF Insert Sequence:

ATGAGGGCCG TGTTGACGTG GAGAGACAAA GCGGAGCAGT GTATCTACGA CCTCGCATTT 
AAGCCTGATG GAACACAACT GATTCTGGCT GCAGGAAACA GATTGCTGGT TTATGACACC 
TCTGATGGCA CCTTACTTCA GCCCCTGAAG GGACACAAAG ACACCGTGTA CTGTGTGGCG 
TATGCAAAGG ATGGCAAGCG CTTTGCATCT GGATCTGCTG ACAAGAGCAT CATTATCTGG 
ACCTCAAAAC TTGAAGGCAT TCTGAAGTAC ACGCACAACG ACTCTATACA GTGTGTCTCC 
TACAATCCTG TTACCCACCA GCTGGCATCT TGTTCCTCCA GTGACTTTGG GTTATGGTCT 
CCTGAGCAGA AGTCTGTGTC CAAGCACAAA TCCAGCAGCA AGATCACGTG CTGCAGCTGG 
ACAAACGATG GTCAGTACCT GGCTCTGGGC ATGGCCAATG GCATCATCAG TATACGGAAC 
AAGAATGGTG AAGAGAAAGT GAAGATCGAG CGCCCTGGGG GCTCCCTCTC CCCAATATGG 
TCCATCTGCT GGAACCCTTC AAGAGAGGAA CATAATGACA TCCTGGCTGT AGCTGACTGG 
GGACAGAAAC TTTCCTTCTA CCAGCTGAGT GGAAAACAGA TTGGGAAGGA TCGGCCACTG 
AACTTTGACC CCTGTTGCAT CAGCTACTTC ACTAAAGGAG AGTACATTTT GGTGGGAGGT 
TCAGACAAGC AGGTATCTCT TTTCACCAAG GATGGAGTGC GACTGGGGAC CGTTGGGGAG 
CAGAACTCCT GGGTGTGGAC CTGTAGAGTG AAACCTGACT CCAATTATGT GGTGGTCGGC 
TGCCAGGATG GCACCATCTC CTTCTACCAG CTCATCTTCA GCACAGTCCA TGGGCTCTAC 
AAAGATCGGT ATGCCTACAG GGACAGCATG ACAGACGTCA TCGTGCAGCA CCTCATCACA 
GAGCAGAAAG TTAGGATTAA GTGCAGAGAG CTTGTCAAGA AAATTGCCAT CTACAAAAAT 
CGATTAGCTA TCCAGCTGCC AGAGAAGATC CTCATCTACG AGCTGTACTC TGAAGACTCG 
ACAGACATGC ACTACCGGGT AAAGGAGAAA ATTGTCAAGA AGTTTGAGTG CAACCTGCTG 
GTGGTGTGTG CTGACCACAT CATCCTGTGC CAGGAGAAAC GGCTACAGTG CCTGTCCTTC 
AGTGGAGTGA AGGAAAGGGA ATGGCAGATG GAATCTCTCA TCCGCTACAT CAAGGTGATC 
GGTGGCCCTG CTGGAAGAGA AGGTCTGTTG GTAGGCCTGA AAAACGGCCA GATCCTGAAG 
ATCTTTGTGG ACAATCTTTT TGCTATTGTC CTGCTGAAGC AGGCCACAGC CGTGCGCTGC 
CTGGACATGA GTGCCTCCCG GAACAAGCTG GCTGTGGTGG ATGAGAATGA CACGTGCCTG 
GTGTACGACA TCCACACGAA GGAACTGCTT TTCCAGGAAC CGAATGCTAA CAGCGTGGCC 
TGGAACACGC AGTGTGAGGA CATGCTCTGC TTCTCTGGAG GAGGCTACCT CAACATCAAG 
GCCAGCACCT TCCCAGTGCA CCAGCAGAAG CTGCAGGGCT TTGTGGTTGG CTACAACGGC 
TCCAAGATCT TCTGCCTTCA TGTCTTCTCT ATGTCTGCCG TGGAGGTGCC ACAGTCCGCT 
CCAATGTACC AGTACCTCGA TAGGAAGATG TTCAAAGAAG CATATCAGAT CGCTTGCTTG 
GGAGTGACAG ACGCTGATTG GCGTGAACTG GCCATGGAAG CTCTAGAAGG ACTAGAGTTT 
GAGACGGCAA GGAAGGCCTT CACCAGAGTC CAGGACCTTC GATACCTAGA ACTGATCAGC 
AGTATTGAGG AGAGGAAGAA GAGGGGAGAG ACCAACAATG ACCTGTTTCT GGCAGATGTG 
TTTTCCTACC AGGGGAAGTT TCATGAAGCC GCCAAACTAT ACAAGAGGAG CGGGCATGAG 
AACCTCGCTC TCGACATGTA TACTGACCTC TGCATGTTTG AGTACGCCAA GGATTTCCTT 
GGATCTGGAG ACCCTAAAGA AACGAAGATG CTCATCACCA AGCAGGCCGA CTGGGCCAGA 
AACATCAATG AGCCCAAAGC TGCAGTGGAG ATGTACATCT CAGCAGGGGA GCACGCCAAG 
GCCATAGAGA TCAGCGGCAG CCATGGCTGG GTGGACATGT TGATAGACAT TGCTCGGAAG 
CTGGACAAGG CTGAACGGGA GCCCCTGCTC ATGTGTGCTT GCTACTTTAA GAAGCTGGAC 
AGTCCTGGCT ATGCTGCTGA GACCTACCTG AAGATCGGGG ACCTGAAGTC TCTGGTGCAG 
CTGTATGTGG ACACCAAGCG CTGGGATGAG GCCTTTGCGT TAGGTGAGAA GCACCCGGAG 
TTCAAGGATG ACGTCTACGT GCCCTACGCG CAGTGGCTGG CGGAGAACGA CCGCTTTGAG 
GAAGCCCAGA AAGCCTTCCA CAAGGCCGGG AGGCAGGGAG AAGCAGTCCG GGTGCTGGAG 
CAGCTCACGC ACAATGCTGT GGTGGAGAGC AGGTTTAACG ACGCTGCCTA TTATTACTGG 
ATGCTGTCCA TGCAGTGCCT CGACATGGCT CAAGATCCTG CCCAGAAGGA CGCGATGCTG 
GACAAGTTTC ACCACTTCCA ACACCTGGCT GAGCTGTACC ATGGCTATCA AACCATTCAC 
CGATACACGG AGGAGCCATT CAGCTTTGAC CTCCCTGAAA CCCTCTTCAA CATCTCCAAG 
TTCTTGCTGC ACAGCCTGAC CAAGGCCACC CCCTTGGGTA TCTCCAAAGT GAATACACTC 
TTCACACTGG CTAAGCAGAG CAAGGCTCTG GGTGCTTACA AGCTGGCCCG GCATGCCTAT 
GACAAGCTAC GGGGGCTGCA GATCCCCGCC AGGATCCAGA AGTCCATTGA GCTGGGCACC 
CTGACCATCC GCTCCAAGCC CTTCCATGAC AGTGAGGAGC TGGTACCCTT GTGCTACCGC 
TGTTCTACCA ACAACCCGCT GCTGAACAAC CTTGGCAACG TCTGCATCAA CTGCCGCCAG 
CCCTTCATCT TCTCCGCCTC TTCCTATGAG GTGCTGCACC TGGTGGAATT CTACCTGGAA 
GAGGGGATCA CTGACGAAGA AGCTGTTGCC CTCATCGATC TGGAGGCACC AAGACACAAG 
AGGGAGGGCA AGTGGCGAGA GACCTCAAGC AACAATTCCC AGACGTTGAA GCTGGACGAG 
ACCATGGACT CCATAGGAGA AGATGACCCA TTTACAGCCA AGCTGAGCTT TGAGCAAGGT 
AGCTCTGAGT TTGTGCCTGT GGTGGTGAAC CGGTCAGTAC TACGCTCCAT GAGCCGAAGA 
GATGTTCTCA TCAAGCGCTG GCCCCCGCCG CTGCAATGGC AATATTTCCG CTCGCTGTTG 
CCTGATGCCT CCATCACCAT GTGCCCCTCC TGCTTTCAGA TGTTCCATTC AGAGGACTAC 
GAACTGCTTG TGCTCCAGCA CGCCTGCTGC CCTTACTGCC GAAGGCGTAT CGACGACACT 
GGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ift122 ( XM_006506797.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506860.1 Mus musculus intraflagellar transport 122 (Ift122), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu37692
Clone ID Related Accession (Same CDS sequence)	XM_006506797.2
Accession Version	XM_006506797.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	intraflagellar transport protein 122 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506797.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCCG TGTTGACGTG GAGAGACAAA GCGGAGCAGT GTATCTACGA CCTCGCATTT 
AAGCCTGATG GAACACAACT GATTCTGGCT GCAGGAAACA GATTGCTGGT TTATGACACC 
TCTGATGGCA CCTTACTTCA GCCCCTGAAG GGACACAAAG ACACCGTGTA CTGTGTGGCG 
TATGCAAAGG ATGGCAAGCG CTTTGCATCT GGATCTGCTG ACAAGAGCAT CATTATCTGG 
ACCTCAAAAC TTGAAGGCAT TCTGAAGTAC ACGCACAACG ACTCTATACA GTGTGTCTCC 
TACAATCCTG TTACCCACCA GCTGGCATCT TGTTCCTCCA GTGACTTTGG GTTATGGTCT 
CCTGAGCAGA AGTCTGTGTC CAAGCACAAA TCCAGCAGCA AGATCACGTG CTGCAGCTGG 
ACAAACGATG GTCAGTACCT GGCTCTGGGC ATGGCCAATG GCATCATCAG TATACGGAAC 
AAGAATGGTG AAGAGAAAGT GAAGATCGAG CGCCCTGGGG GCTCCCTCTC CCCAATATGG 
TCCATCTGCT GGAACCCTTC AAGCCGATGG GAGACTATCT GGATGAGCAG AGAGAACGAG 
GATGCTGAGG ATGTCATTGT CAACAAATAT GTTCAGGAAA TCCCTTCCAT TCTGAAGTCA 
ACAGTGTACA ATAGTCAGGG TAGTGAGGCA GAGGAGGAAG AGCAAGAGGA AGAGGACGAC 
AGTCCCAGGG ACGGCAACTC AGAGGAACAT AATGACATCC TGGCTGTAGC TGACTGGGGA 
CAGAAACTTT CCTTCTACCA GCTGAGTGGA AAACAGATTG GGAAGGATCG GCCACTGAAC 
TTTGACCCCT GTTGCATCAG CTACTTCACT AAAGGAGAGT ACATTTTGGT GGGAGGTTCA 
GACAAGCAGG TATCTCTTTT CACCAAGGAT GGAGTGCGAC TGGGGACCGT TGGGGAGCAG 
AACTCCTGGG TGTGGACCTG TAGAGTGAAA CCTGACTCCA ATTATGTGGT GGTCGGCTGC 
CAGGATGGCA CCATCTCCTT CTACCAGCTC ATCTTCAGCA CAGTCCATGG GCTCTACAAA 
GATCGGTATG CCTACAGGGA CAGCATGACA GACGTCATCG TGCAGCACCT CATCACAGAG 
CAGAAAGTTA GGATTAAGTG CAGAGAGCTT GTCAAGAAAA TTGCCATCTA CAAAAATCGA 
TTAGCTATCC AGCTGCCAGA GAAGATCCTC ATCTACGAGC TGTACTCTGA AGACTCGACA 
GACATGCACT ACCGGGTAAA GGAGAAAATT GTCAAGAAGT TTGAGTGCAA CCTGCTGGTG 
GTGTGTGCTG ACCACATCAT CCTGTGCCAG GAGAAACGGC TACAGTGCCT GTCCTTCAGT 
GGAGTGAAGG AAAGGGAATG GCAGATGGAA TCTCTCATCC GCTACATCAA GGTGATCGGT 
GGCCCTGCTG GAAGAGAAGG TCTGTTGGTA GGCCTGAAAA ACGGCCAGAT CCTGAAGATC 
TTTGTGGACA ATCTTTTTGC TATTGTCCTG CTGAAGCAGG CCACAGCCGT GCGCTGCCTG 
GACATGAGTG CCTCCCGGAA CAAGCTGGCT GTGGTGGATG AGAATGACAC GTGCCTGGTG 
TACGACATCC ACACGAAGGA ACTGCTTTTC CAGGAACCGA ATGCTAACAG CGTGGCCTGG 
AACACGCAGT GTGAGGACAT GCTCTGCTTC TCTGGAGGAG GCTACCTCAA CATCAAGGCC 
AGCACCTTCC CAGTGCACCA GCAGAAGCTG CAGGGCTTTG TGGTTGGCTA CAACGGCTCC 
AAGATCTTCT GCCTTCATGT CTTCTCTATG TCTGCCGTGG AGGTGCCACA GTCCGCTCCA 
ATGTACCAGT ACCTCGATAG GAAGATGTTC AAAGAAGCAT ATCAGATCGC TTGCTTGGGA 
GTGACAGACG CTGATTGGCG TGAACTGGCC ATGGAAGCTC TAGAAGGACT AGAGTTTGAG 
ACGGCAAGGA AGGCCTTCAC CAGAGTCCAG GACCTTCGAT ACCTAGAACT GATCAGCAGT 
ATTGAGGAGA GGAAGAAGAG GGGAGAGACC AACAATGACC TGTTTCTGGC AGATGTGTTT 
TCCTACCAGG GGAAGTTTCA TGAAGCCGCC AAACTATACA AGAGGAGCGG GCATGAGAAC 
CTCGCTCTCG ACATGTATAC TGACCTCTGC ATGTTTGAGT ACGCCAAGGA TTTCCTTGGA 
TCTGGAGACC CTAAAGAAAC GAAGATGCTC ATCACCAAGC AGGCCGACTG GGCCAGAAAC 
ATCAATGAGC CCAAAGCTGC AGTGGAGATG TACATCTCAG CAGGGGAGCA CGCCAAGGCC 
ATAGAGATCA GCGGCAGCCA TGGCTGGGTG GACATGTTGA TAGACATTGC TCGGAAGCTG 
GACAAGGCTG AACGGGAGCC CCTGCTCATG TGTGCTTGCT ACTTTAAGAA GCTGGACAGT 
CCTGGCTATG CTGCTGAGAC CTACCTGAAG ATCGGGGACC TGAAGTCTCT GGTGCAGCTG 
TATGTGGACA CCAAGCGCTG GGATGAGGCC TTTGCGTTAG GTGAGAAGCA CCCGGAGTTC 
AAGGATGACG TCTACGTGCC CTACGCGCAG TGGCTGGCGG AGAACGACCG CTTTGAGGAA 
GCCCAGAAAG CCTTCCACAA GGCCGGGAGG CAGGGAGAAG CAGTCCGGGT GCTGGAGCAG 
CTCACGCACA ATGCTGTGGT GGAGAGCAGG TTTAACGACG CTGCCTATTA TTACTGGATG 
CTGTCCATGC AGTGCCTCGA CATGGCTCAA GCAGATCCTG CCCAGAAGGA CGCGATGCTG 
GACAAGTTTC ACCACTTCCA ACACCTGGCT GAGCTGTACC ATGGCTATCA AACCATTCAC 
CGATACACGG AGGAGCCATT CAGCTTTGAC CTCCCTGAAA CCCTCTTCAA CATCTCCAAG 
TTCTTGCTGC ACAGCCTGAC CAAGGCCACC CCCTTGGGTA TCTCCAAAGT GAATACACTC 
TTCACACTGG CTAAGCAGAG CAAGGCTCTG GGTGCTTACA AGCTGGCCCG GCATGCCTAT 
GACAAGCTAC GGGGGCTGCA GATCCCCGCC AGGATCCAGA AGTCCATTGA GCTGGGCACC 
CTGACCATCC GCTCCAAGCC CTTCCATGAC AGTGAGGAGC TGGTACCCTT GTGCTACCGC 
TGTTCTACCA ACAACCCGCT GCTGAACAAC CTTGGCAACG TCTGCATCAA CTGCCGCCAG 
CCCTTCATCT TCTCCGCCTC TTCCTATGAG GTGCTGCACC TGGTGGAATT CTACCTGGAA 
GAGGGGATCA CTGACGAAGA AGCTGTTGCC CTCATCGATC TGGAGGCACC AAGACACAAG 
AGGGAGGGCA AGTGGCGAGA GACCTCAAGC AACAATTCCC AGACGTTGAA GCTGGACGAG 
ACCATGGACT CCATAGGAGA AGATGACCCA TTTACAGCCA AGCTGAGCTT TGAGCAAGGT 
AGCTCTGAGT TTGTGCCTGT GGTGGTGAAC CGGTCAGTAC TACGCTCCAT GAGCCGAAGA 
GATGTTCTCA TCAAGCGCTG GCCCCCGCCG CTGCAATGGC AATATTTCCG CTCGCTGTTG 
CCTGATGCCT CCATCACCAT GTGCCCCTCC TGCTTTCAGA TGTTCCATTC AGAGGACTAC 
GAACTGCTTG TGCTCCAGCA CGCCTGCTGC CCTTACTGCC GAAGGCGTAT CGACGACACT 
GGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506860.1
CDS	195..3923
Translation	MRAVLTWRDKAEQCIYDLAFKPDGTQLILAAGNRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGKRFASGSADKSIIIWTSKLEGILKYTHNDSIQCVSYNPVTHQLASCSSSDFGLWSPEQKSVSKHKSSSKITCCSWTNDGQYLALGMANGIISIRNKNGEEKVKIERPGGSLSPIWSICWNPSSRWETIWMSRENEDAEDVIVNKYVQEIPSILKSTVYNSQGSEAEEEEQEEEDDSPRDGNSEEHNDILAVADWGQKLSFYQLSGKQIGKDRPLNFDPCCISYFTKGEYILVGGSDKQVSLFTKDGVRLGTVGEQNSWVWTCRVKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCRELVKKIAIYKNRLAIQLPEKILIYELYSEDSTDMHYRVKEKIVKKFECNLLVVCADHIILCQEKRLQCLSFSGVKEREWQMESLIRYIKVIGGPAGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRNKLAVVDENDTCLVYDIHTKELLFQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHQQKLQGFVVGYNGSKIFCLHVFSMSAVEVPQSAPMYQYLDRKMFKEAYQIACLGVTDADWRELAMEALEGLEFETARKAFTRVQDLRYLELISSIEERKKRGETNNDLFLADVFSYQGKFHEAAKLYKRSGHENLALDMYTDLCMFEYAKDFLGSGDPKETKMLITKQADWARNINEPKAAVEMYISAGEHAKAIEISGSHGWVDMLIDIARKLDKAEREPLLMCACYFKKLDSPGYAAETYLKIGDLKSLVQLYVDTKRWDEAFALGEKHPEFKDDVYVPYAQWLAENDRFEEAQKAFHKAGRQGEAVRVLEQLTHNAVVESRFNDAAYYYWMLSMQCLDMAQADPAQKDAMLDKFHHFQHLAELYHGYQTIHRYTEEPFSFDLPETLFNISKFLLHSLTKATPLGISKVNTLFTLAKQSKALGAYKLARHAYDKLRGLQIPARIQKSIELGTLTIRSKPFHDSEELVPLCYRCSTNNPLLNNLGNVCINCRQPFIFSASSYEVLHLVEFYLEEGITDEEAVALIDLEAPRHKREGKWRETSSNNSQTLKLDETMDSIGEDDPFTAKLSFEQGSSEFVPVVVNRSVLRSMSRRDVLIKRWPPPLQWQYFRSLLPDASITMCPSCFQMFHSEDYELLVLQHACCPYCRRRIDDTGP

Target ORF information:

RefSeq Version	XM_006506797.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus intraflagellar transport 122 (Ift122), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506797.2

ORF Insert Sequence:

ATGAGGGCCG TGTTGACGTG GAGAGACAAA GCGGAGCAGT GTATCTACGA CCTCGCATTT 
AAGCCTGATG GAACACAACT GATTCTGGCT GCAGGAAACA GATTGCTGGT TTATGACACC 
TCTGATGGCA CCTTACTTCA GCCCCTGAAG GGACACAAAG ACACCGTGTA CTGTGTGGCG 
TATGCAAAGG ATGGCAAGCG CTTTGCATCT GGATCTGCTG ACAAGAGCAT CATTATCTGG 
ACCTCAAAAC TTGAAGGCAT TCTGAAGTAC ACGCACAACG ACTCTATACA GTGTGTCTCC 
TACAATCCTG TTACCCACCA GCTGGCATCT TGTTCCTCCA GTGACTTTGG GTTATGGTCT 
CCTGAGCAGA AGTCTGTGTC CAAGCACAAA TCCAGCAGCA AGATCACGTG CTGCAGCTGG 
ACAAACGATG GTCAGTACCT GGCTCTGGGC ATGGCCAATG GCATCATCAG TATACGGAAC 
AAGAATGGTG AAGAGAAAGT GAAGATCGAG CGCCCTGGGG GCTCCCTCTC CCCAATATGG 
TCCATCTGCT GGAACCCTTC AAGCCGATGG GAGACTATCT GGATGAGCAG AGAGAACGAG 
GATGCTGAGG ATGTCATTGT CAACAAATAT GTTCAGGAAA TCCCTTCCAT TCTGAAGTCA 
ACAGTGTACA ATAGTCAGGG TAGTGAGGCA GAGGAGGAAG AGCAAGAGGA AGAGGACGAC 
AGTCCCAGGG ACGGCAACTC AGAGGAACAT AATGACATCC TGGCTGTAGC TGACTGGGGA 
CAGAAACTTT CCTTCTACCA GCTGAGTGGA AAACAGATTG GGAAGGATCG GCCACTGAAC 
TTTGACCCCT GTTGCATCAG CTACTTCACT AAAGGAGAGT ACATTTTGGT GGGAGGTTCA 
GACAAGCAGG TATCTCTTTT CACCAAGGAT GGAGTGCGAC TGGGGACCGT TGGGGAGCAG 
AACTCCTGGG TGTGGACCTG TAGAGTGAAA CCTGACTCCA ATTATGTGGT GGTCGGCTGC 
CAGGATGGCA CCATCTCCTT CTACCAGCTC ATCTTCAGCA CAGTCCATGG GCTCTACAAA 
GATCGGTATG CCTACAGGGA CAGCATGACA GACGTCATCG TGCAGCACCT CATCACAGAG 
CAGAAAGTTA GGATTAAGTG CAGAGAGCTT GTCAAGAAAA TTGCCATCTA CAAAAATCGA 
TTAGCTATCC AGCTGCCAGA GAAGATCCTC ATCTACGAGC TGTACTCTGA AGACTCGACA 
GACATGCACT ACCGGGTAAA GGAGAAAATT GTCAAGAAGT TTGAGTGCAA CCTGCTGGTG 
GTGTGTGCTG ACCACATCAT CCTGTGCCAG GAGAAACGGC TACAGTGCCT GTCCTTCAGT 
GGAGTGAAGG AAAGGGAATG GCAGATGGAA TCTCTCATCC GCTACATCAA GGTGATCGGT 
GGCCCTGCTG GAAGAGAAGG TCTGTTGGTA GGCCTGAAAA ACGGCCAGAT CCTGAAGATC 
TTTGTGGACA ATCTTTTTGC TATTGTCCTG CTGAAGCAGG CCACAGCCGT GCGCTGCCTG 
GACATGAGTG CCTCCCGGAA CAAGCTGGCT GTGGTGGATG AGAATGACAC GTGCCTGGTG 
TACGACATCC ACACGAAGGA ACTGCTTTTC CAGGAACCGA ATGCTAACAG CGTGGCCTGG 
AACACGCAGT GTGAGGACAT GCTCTGCTTC TCTGGAGGAG GCTACCTCAA CATCAAGGCC 
AGCACCTTCC CAGTGCACCA GCAGAAGCTG CAGGGCTTTG TGGTTGGCTA CAACGGCTCC 
AAGATCTTCT GCCTTCATGT CTTCTCTATG TCTGCCGTGG AGGTGCCACA GTCCGCTCCA 
ATGTACCAGT ACCTCGATAG GAAGATGTTC AAAGAAGCAT ATCAGATCGC TTGCTTGGGA 
GTGACAGACG CTGATTGGCG TGAACTGGCC ATGGAAGCTC TAGAAGGACT AGAGTTTGAG 
ACGGCAAGGA AGGCCTTCAC CAGAGTCCAG GACCTTCGAT ACCTAGAACT GATCAGCAGT 
ATTGAGGAGA GGAAGAAGAG GGGAGAGACC AACAATGACC TGTTTCTGGC AGATGTGTTT 
TCCTACCAGG GGAAGTTTCA TGAAGCCGCC AAACTATACA AGAGGAGCGG GCATGAGAAC 
CTCGCTCTCG ACATGTATAC TGACCTCTGC ATGTTTGAGT ACGCCAAGGA TTTCCTTGGA 
TCTGGAGACC CTAAAGAAAC GAAGATGCTC ATCACCAAGC AGGCCGACTG GGCCAGAAAC 
ATCAATGAGC CCAAAGCTGC AGTGGAGATG TACATCTCAG CAGGGGAGCA CGCCAAGGCC 
ATAGAGATCA GCGGCAGCCA TGGCTGGGTG GACATGTTGA TAGACATTGC TCGGAAGCTG 
GACAAGGCTG AACGGGAGCC CCTGCTCATG TGTGCTTGCT ACTTTAAGAA GCTGGACAGT 
CCTGGCTATG CTGCTGAGAC CTACCTGAAG ATCGGGGACC TGAAGTCTCT GGTGCAGCTG 
TATGTGGACA CCAAGCGCTG GGATGAGGCC TTTGCGTTAG GTGAGAAGCA CCCGGAGTTC 
AAGGATGACG TCTACGTGCC CTACGCGCAG TGGCTGGCGG AGAACGACCG CTTTGAGGAA 
GCCCAGAAAG CCTTCCACAA GGCCGGGAGG CAGGGAGAAG CAGTCCGGGT GCTGGAGCAG 
CTCACGCACA ATGCTGTGGT GGAGAGCAGG TTTAACGACG CTGCCTATTA TTACTGGATG 
CTGTCCATGC AGTGCCTCGA CATGGCTCAA GCAGATCCTG CCCAGAAGGA CGCGATGCTG 
GACAAGTTTC ACCACTTCCA ACACCTGGCT GAGCTGTACC ATGGCTATCA AACCATTCAC 
CGATACACGG AGGAGCCATT CAGCTTTGAC CTCCCTGAAA CCCTCTTCAA CATCTCCAAG 
TTCTTGCTGC ACAGCCTGAC CAAGGCCACC CCCTTGGGTA TCTCCAAAGT GAATACACTC 
TTCACACTGG CTAAGCAGAG CAAGGCTCTG GGTGCTTACA AGCTGGCCCG GCATGCCTAT 
GACAAGCTAC GGGGGCTGCA GATCCCCGCC AGGATCCAGA AGTCCATTGA GCTGGGCACC 
CTGACCATCC GCTCCAAGCC CTTCCATGAC AGTGAGGAGC TGGTACCCTT GTGCTACCGC 
TGTTCTACCA ACAACCCGCT GCTGAACAAC CTTGGCAACG TCTGCATCAA CTGCCGCCAG 
CCCTTCATCT TCTCCGCCTC TTCCTATGAG GTGCTGCACC TGGTGGAATT CTACCTGGAA 
GAGGGGATCA CTGACGAAGA AGCTGTTGCC CTCATCGATC TGGAGGCACC AAGACACAAG 
AGGGAGGGCA AGTGGCGAGA GACCTCAAGC AACAATTCCC AGACGTTGAA GCTGGACGAG 
ACCATGGACT CCATAGGAGA AGATGACCCA TTTACAGCCA AGCTGAGCTT TGAGCAAGGT 
AGCTCTGAGT TTGTGCCTGT GGTGGTGAAC CGGTCAGTAC TACGCTCCAT GAGCCGAAGA 
GATGTTCTCA TCAAGCGCTG GCCCCCGCCG CTGCAATGGC AATATTTCCG CTCGCTGTTG 
CCTGATGCCT CCATCACCAT GTGCCCCTCC TGCTTTCAGA TGTTCCATTC AGAGGACTAC 
GAACTGCTTG TGCTCCAGCA CGCCTGCTGC CCTTACTGCC GAAGGCGTAT CGACGACACT 
GGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ift122 ( XM_006506798.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506861.1 Mus musculus intraflagellar transport 122 (Ift122), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu37693
Clone ID Related Accession (Same CDS sequence)	XM_006506798.2
Accession Version	XM_006506798.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	intraflagellar transport protein 122 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506798.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCCG TGTTGACGTG GAGAGACAAA GCGGAGCAGT GTATCTACGA CCTCGCATTT 
AAGCCTGATG GAACACAACT GATTCTGGCT GCAGGAAACA GATTGCTGGT TTATGACACC 
TCTGATGGCA CCTTACTTCA GCCCCTGAAG GGACACAAAG ACACCGTGTA CTGTGTGGCG 
TATGCAAAGG ATGGCAAGCG CTTTGCATCT GGATCTGCTG ACAAGAGCAT CATTATCTGG 
ACCTCAAAAC TTGAAGGCAT TCTGAAGTAC ACGCACAACG ACTCTATACA GTGTGTCTCC 
TACAATCCTG TTACCCACCA GCTGGCATCT TGTTCCTCCA GTGACTTTGG GTTATGGTCT 
CCTGAGCAGA AGTCTGTGTC CAAGCACAAA TCCAGCAGCA AGATCACGTG CTGCAGCTGG 
ACAAACGATG GTCAGTACCT GGCTCTGGGC ATGGCCAATG GCATCATCAG TATACGGAAC 
AAGAATGGTG AAGAGAAAGT GAAGATCGAG CGCCCTGGGG GCTCCCTCTC CCCAATATGG 
TCCATCTGCT GGAACCCTTC AAGCCGATGG GAGACTATCT GGATGAGCAG AGAGAACGAG 
GATGCTGAGG ATGTCATTGT CAACAAATAT GTTCAGGAAA TCCCTTCCAT TCTGAAGTCA 
ACAGTGTACA ATAGTCAGGG TAGTGAGGCA GAGGAGGAAG AGCAAGAGGA AGAGGACGAC 
AGTCCCAGGG ACGGCAACTC AGAGGAACAT AATGACATCC TGGCTGTAGC TGACTGGGGA 
CAGAAACTTT CCTTCTACCA GCTGAGTGGA AAACAGATTG GGAAGGATCG GCCACTGAAC 
TTTGACCCCT GTTGCATCAG CTACTTCACT AAAGGAGAGT ACATTTTGGT GGGAGGTTCA 
GACAAGCAGG TATCTCTTTT CACCAAGGAT GGAGTGCGAC TGGGGACCGT TGGGGAGCAG 
AACTCCTGGG TGTGGACCTG TAGAGTGAAA CCTGACTCCA ATTATGTGGT GGTCGGCTGC 
CAGGATGGCA CCATCTCCTT CTACCAGCTC ATCTTCAGCA CAGTCCATGG GCTCTACAAA 
GATCGGTATG CCTACAGGGA CAGCATGACA GACGTCATCG TGCAGCACCT CATCACAGAG 
CAGAAAGTTA GGATTAAGTG CAGAGAGCTT GTCAAGAAAA TTGCCATCTA CAAAAATCGA 
TTAGCTATCC AGCTGCCAGA GAAGATCCTC ATCTACGAGC TGTACTCTGA AGACTCGACA 
GACATGCACT ACCGGGTAAA GGAGAAAATT GTCAAGAAGT TTGAGTGCAA CCTGCTGGTG 
GTGTGTGCTG ACCACATCAT CCTGTGCCAG GAGAAACGGC TACAGTGCCT GTCCTTCAGT 
GGAGTGAAGG AAAGGGAATG GCAGATGGAA TCTCTCATCC GCTACATCAA GGTGATCGGT 
GGCCCTGCTG GAAGAGAAGG TCTGTTGGTA GGCCTGAAAA ACGGCCAGAT CCTGAAGATC 
TTTGTGGACA ATCTTTTTGC TATTGTCCTG CTGAAGCAGG CCACAGCCGT GCGCTGCCTG 
GACATGAGTG CCTCCCGGAA CAAGCTGGCT GTGGTGGATG AGAATGACAC GTGCCTGGTG 
TACGACATCC ACACGAAGGA ACTGCTTTTC CAGGAACCGA ATGCTAACAG CGTGGCCTGG 
AACACGCAGT GTGAGGACAT GCTCTGCTTC TCTGGAGGAG GCTACCTCAA CATCAAGGCC 
AGCACCTTCC CAGTGCACCA GCAGAAGCTG CAGGGCTTTG TGGTTGGCTA CAACGGCTCC 
AAGATCTTCT GCCTTCATGT CTTCTCTATG TCTGCCGTGG AGGTGCCACA GTCCGCTCCA 
ATGTACCAGT ACCTCGATAG GAAGATGTTC AAAGAAGCAT ATCAGATCGC TTGCTTGGGA 
GTGACAGACG CTGATTGGCG TGAACTGGCC ATGGAAGCTC TAGAAGGACT AGAGTTTGAG 
ACGGCAAGGA AGGCCTTCAC CAGAGTCCAG GACCTTCGAT ACCTAGAACT GATCAGCAGT 
ATTGAGGAGA GGAAGAAGAG GGGAGAGACC AACAATGACC TGTTTCTGGC AGATGTGTTT 
TCCTACCAGG GGAAGTTTCA TGAAGCCGCC AAACTATACA AGAGGAGCGG GCATGAGAAC 
CTCGCTCTCG ACATGTATAC TGACCTCTGC ATGTTTGAGT ACGCCAAGGA TTTCCTTGGA 
TCTGGAGACC CTAAAGAAAC GAAGATGCTC ATCACCAAGC AGGCCGACTG GGCCAGAAAC 
ATCAATGAGC CCAAAGCTGC AGTGGAGATG TACATCTCAG CAGGGGAGCA CGCCAAGGCC 
ATAGAGATCA GCGGCAGCCA TGGCTGGGTG GACATGTTGA TAGACATTGC TCGGAAGCTG 
GACAAGGCTG AACGGGAGCC CCTGCTCATG TGTGCTTGCT ACTTTAAGAA GCTGGACAGT 
CCTGGCTATG CTGCTGAGAC CTACCTGAAG ATCGGGGACC TGAAGTCTCT GGTGCAGCTG 
TATGTGGACA CCAAGCGCTG GGATGAGGCC TTTGCGTTAG GTGAGAAGCA CCCGGAGTTC 
AAGGATGACG TCTACGTGCC CTACGCGCAG TGGCTGGCGG AGAACGACCG CTTTGAGGAA 
GCCCAGAAAG CCTTCCACAA GGCCGGGAGG CAGGGAGAAG CAGTCCGGGT GCTGGAGCAG 
CTCACGCACA ATGCTGTGGT GGAGAGCAGG TTTAACGACG CTGCCTATTA TTACTGGATG 
CTGTCCATGC AGTGCCTCGA CATGGCTCAA GATCCTGCCC AGAAGGACGC GATGCTGGAC 
AAGTTTCACC ACTTCCAACA CCTGGCTGAG CTGTACCATG GCTATCAAAC CATTCACCGA 
TACACGGAGG AGCCATTCAG CTTTGACCTC CCTGAAACCC TCTTCAACAT CTCCAAGTTC 
TTGCTGCACA GCCTGACCAA GGCCACCCCC TTGGGTATCT CCAAAGTGAA TACACTCTTC 
ACACTGGCTA AGCAGAGCAA GGCTCTGGGT GCTTACAAGC TGGCCCGGCA TGCCTATGAC 
AAGCTACGGG GGCTGCAGAT CCCCGCCAGG ATCCAGAAGT CCATTGAGCT GGGCACCCTG 
ACCATCCGCT CCAAGCCCTT CCATGACAGT GAGGAGCTGG TACCCTTGTG CTACCGCTGT 
TCTACCAACA ACCCGCTGCT GAACAACCTT GGCAACGTCT GCATCAACTG CCGCCAGCCC 
TTCATCTTCT CCGCCTCTTC CTATGAGGTG CTGCACCTGG TGGAATTCTA CCTGGAAGAG 
GGGATCACTG ACGAAGAAGC TGTTGCCCTC ATCGATCTGG AGGCACCAAG ACACAAGAGG 
GAGGGCAAGT GGCGAGAGAC CTCAAGCAAC AATTCCCAGA CGTTGAAGCT GGACGAGACC 
ATGGACTCCA TAGGAGAAGA TGACCCATTT ACAGCCAAGC TGAGCTTTGA GCAAGGTAGC 
TCTGAGTTTG TGCCTGTGGT GGTGAACCGG TCAGTACTAC GCTCCATGAG CCGAAGAGAT 
GTTCTCATCA AGCGCTGGCC CCCGCCGCTG CAATGGCAAT ATTTCCGCTC GCTGTTGCCT 
GATGCCTCCA TCACCATGTG CCCCTCCTGC TTTCAGATGT TCCATTCAGA GGACTACGAA 
CTGCTTGTGC TCCAGCACGC CTGCTGCCCT TACTGCCGAA GGCGTATCGA CGACACTGGC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506861.1
CDS	195..3920
Translation	MRAVLTWRDKAEQCIYDLAFKPDGTQLILAAGNRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGKRFASGSADKSIIIWTSKLEGILKYTHNDSIQCVSYNPVTHQLASCSSSDFGLWSPEQKSVSKHKSSSKITCCSWTNDGQYLALGMANGIISIRNKNGEEKVKIERPGGSLSPIWSICWNPSSRWETIWMSRENEDAEDVIVNKYVQEIPSILKSTVYNSQGSEAEEEEQEEEDDSPRDGNSEEHNDILAVADWGQKLSFYQLSGKQIGKDRPLNFDPCCISYFTKGEYILVGGSDKQVSLFTKDGVRLGTVGEQNSWVWTCRVKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCRELVKKIAIYKNRLAIQLPEKILIYELYSEDSTDMHYRVKEKIVKKFECNLLVVCADHIILCQEKRLQCLSFSGVKEREWQMESLIRYIKVIGGPAGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRNKLAVVDENDTCLVYDIHTKELLFQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHQQKLQGFVVGYNGSKIFCLHVFSMSAVEVPQSAPMYQYLDRKMFKEAYQIACLGVTDADWRELAMEALEGLEFETARKAFTRVQDLRYLELISSIEERKKRGETNNDLFLADVFSYQGKFHEAAKLYKRSGHENLALDMYTDLCMFEYAKDFLGSGDPKETKMLITKQADWARNINEPKAAVEMYISAGEHAKAIEISGSHGWVDMLIDIARKLDKAEREPLLMCACYFKKLDSPGYAAETYLKIGDLKSLVQLYVDTKRWDEAFALGEKHPEFKDDVYVPYAQWLAENDRFEEAQKAFHKAGRQGEAVRVLEQLTHNAVVESRFNDAAYYYWMLSMQCLDMAQDPAQKDAMLDKFHHFQHLAELYHGYQTIHRYTEEPFSFDLPETLFNISKFLLHSLTKATPLGISKVNTLFTLAKQSKALGAYKLARHAYDKLRGLQIPARIQKSIELGTLTIRSKPFHDSEELVPLCYRCSTNNPLLNNLGNVCINCRQPFIFSASSYEVLHLVEFYLEEGITDEEAVALIDLEAPRHKREGKWRETSSNNSQTLKLDETMDSIGEDDPFTAKLSFEQGSSEFVPVVVNRSVLRSMSRRDVLIKRWPPPLQWQYFRSLLPDASITMCPSCFQMFHSEDYELLVLQHACCPYCRRRIDDTGP

Target ORF information:

RefSeq Version	XM_006506798.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus intraflagellar transport 122 (Ift122), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506798.2

ORF Insert Sequence:

ATGAGGGCCG TGTTGACGTG GAGAGACAAA GCGGAGCAGT GTATCTACGA CCTCGCATTT 
AAGCCTGATG GAACACAACT GATTCTGGCT GCAGGAAACA GATTGCTGGT TTATGACACC 
TCTGATGGCA CCTTACTTCA GCCCCTGAAG GGACACAAAG ACACCGTGTA CTGTGTGGCG 
TATGCAAAGG ATGGCAAGCG CTTTGCATCT GGATCTGCTG ACAAGAGCAT CATTATCTGG 
ACCTCAAAAC TTGAAGGCAT TCTGAAGTAC ACGCACAACG ACTCTATACA GTGTGTCTCC 
TACAATCCTG TTACCCACCA GCTGGCATCT TGTTCCTCCA GTGACTTTGG GTTATGGTCT 
CCTGAGCAGA AGTCTGTGTC CAAGCACAAA TCCAGCAGCA AGATCACGTG CTGCAGCTGG 
ACAAACGATG GTCAGTACCT GGCTCTGGGC ATGGCCAATG GCATCATCAG TATACGGAAC 
AAGAATGGTG AAGAGAAAGT GAAGATCGAG CGCCCTGGGG GCTCCCTCTC CCCAATATGG 
TCCATCTGCT GGAACCCTTC AAGCCGATGG GAGACTATCT GGATGAGCAG AGAGAACGAG 
GATGCTGAGG ATGTCATTGT CAACAAATAT GTTCAGGAAA TCCCTTCCAT TCTGAAGTCA 
ACAGTGTACA ATAGTCAGGG TAGTGAGGCA GAGGAGGAAG AGCAAGAGGA AGAGGACGAC 
AGTCCCAGGG ACGGCAACTC AGAGGAACAT AATGACATCC TGGCTGTAGC TGACTGGGGA 
CAGAAACTTT CCTTCTACCA GCTGAGTGGA AAACAGATTG GGAAGGATCG GCCACTGAAC 
TTTGACCCCT GTTGCATCAG CTACTTCACT AAAGGAGAGT ACATTTTGGT GGGAGGTTCA 
GACAAGCAGG TATCTCTTTT CACCAAGGAT GGAGTGCGAC TGGGGACCGT TGGGGAGCAG 
AACTCCTGGG TGTGGACCTG TAGAGTGAAA CCTGACTCCA ATTATGTGGT GGTCGGCTGC 
CAGGATGGCA CCATCTCCTT CTACCAGCTC ATCTTCAGCA CAGTCCATGG GCTCTACAAA 
GATCGGTATG CCTACAGGGA CAGCATGACA GACGTCATCG TGCAGCACCT CATCACAGAG 
CAGAAAGTTA GGATTAAGTG CAGAGAGCTT GTCAAGAAAA TTGCCATCTA CAAAAATCGA 
TTAGCTATCC AGCTGCCAGA GAAGATCCTC ATCTACGAGC TGTACTCTGA AGACTCGACA 
GACATGCACT ACCGGGTAAA GGAGAAAATT GTCAAGAAGT TTGAGTGCAA CCTGCTGGTG 
GTGTGTGCTG ACCACATCAT CCTGTGCCAG GAGAAACGGC TACAGTGCCT GTCCTTCAGT 
GGAGTGAAGG AAAGGGAATG GCAGATGGAA TCTCTCATCC GCTACATCAA GGTGATCGGT 
GGCCCTGCTG GAAGAGAAGG TCTGTTGGTA GGCCTGAAAA ACGGCCAGAT CCTGAAGATC 
TTTGTGGACA ATCTTTTTGC TATTGTCCTG CTGAAGCAGG CCACAGCCGT GCGCTGCCTG 
GACATGAGTG CCTCCCGGAA CAAGCTGGCT GTGGTGGATG AGAATGACAC GTGCCTGGTG 
TACGACATCC ACACGAAGGA ACTGCTTTTC CAGGAACCGA ATGCTAACAG CGTGGCCTGG 
AACACGCAGT GTGAGGACAT GCTCTGCTTC TCTGGAGGAG GCTACCTCAA CATCAAGGCC 
AGCACCTTCC CAGTGCACCA GCAGAAGCTG CAGGGCTTTG TGGTTGGCTA CAACGGCTCC 
AAGATCTTCT GCCTTCATGT CTTCTCTATG TCTGCCGTGG AGGTGCCACA GTCCGCTCCA 
ATGTACCAGT ACCTCGATAG GAAGATGTTC AAAGAAGCAT ATCAGATCGC TTGCTTGGGA 
GTGACAGACG CTGATTGGCG TGAACTGGCC ATGGAAGCTC TAGAAGGACT AGAGTTTGAG 
ACGGCAAGGA AGGCCTTCAC CAGAGTCCAG GACCTTCGAT ACCTAGAACT GATCAGCAGT 
ATTGAGGAGA GGAAGAAGAG GGGAGAGACC AACAATGACC TGTTTCTGGC AGATGTGTTT 
TCCTACCAGG GGAAGTTTCA TGAAGCCGCC AAACTATACA AGAGGAGCGG GCATGAGAAC 
CTCGCTCTCG ACATGTATAC TGACCTCTGC ATGTTTGAGT ACGCCAAGGA TTTCCTTGGA 
TCTGGAGACC CTAAAGAAAC GAAGATGCTC ATCACCAAGC AGGCCGACTG GGCCAGAAAC 
ATCAATGAGC CCAAAGCTGC AGTGGAGATG TACATCTCAG CAGGGGAGCA CGCCAAGGCC 
ATAGAGATCA GCGGCAGCCA TGGCTGGGTG GACATGTTGA TAGACATTGC TCGGAAGCTG 
GACAAGGCTG AACGGGAGCC CCTGCTCATG TGTGCTTGCT ACTTTAAGAA GCTGGACAGT 
CCTGGCTATG CTGCTGAGAC CTACCTGAAG ATCGGGGACC TGAAGTCTCT GGTGCAGCTG 
TATGTGGACA CCAAGCGCTG GGATGAGGCC TTTGCGTTAG GTGAGAAGCA CCCGGAGTTC 
AAGGATGACG TCTACGTGCC CTACGCGCAG TGGCTGGCGG AGAACGACCG CTTTGAGGAA 
GCCCAGAAAG CCTTCCACAA GGCCGGGAGG CAGGGAGAAG CAGTCCGGGT GCTGGAGCAG 
CTCACGCACA ATGCTGTGGT GGAGAGCAGG TTTAACGACG CTGCCTATTA TTACTGGATG 
CTGTCCATGC AGTGCCTCGA CATGGCTCAA GATCCTGCCC AGAAGGACGC GATGCTGGAC 
AAGTTTCACC ACTTCCAACA CCTGGCTGAG CTGTACCATG GCTATCAAAC CATTCACCGA 
TACACGGAGG AGCCATTCAG CTTTGACCTC CCTGAAACCC TCTTCAACAT CTCCAAGTTC 
TTGCTGCACA GCCTGACCAA GGCCACCCCC TTGGGTATCT CCAAAGTGAA TACACTCTTC 
ACACTGGCTA AGCAGAGCAA GGCTCTGGGT GCTTACAAGC TGGCCCGGCA TGCCTATGAC 
AAGCTACGGG GGCTGCAGAT CCCCGCCAGG ATCCAGAAGT CCATTGAGCT GGGCACCCTG 
ACCATCCGCT CCAAGCCCTT CCATGACAGT GAGGAGCTGG TACCCTTGTG CTACCGCTGT 
TCTACCAACA ACCCGCTGCT GAACAACCTT GGCAACGTCT GCATCAACTG CCGCCAGCCC 
TTCATCTTCT CCGCCTCTTC CTATGAGGTG CTGCACCTGG TGGAATTCTA CCTGGAAGAG 
GGGATCACTG ACGAAGAAGC TGTTGCCCTC ATCGATCTGG AGGCACCAAG ACACAAGAGG 
GAGGGCAAGT GGCGAGAGAC CTCAAGCAAC AATTCCCAGA CGTTGAAGCT GGACGAGACC 
ATGGACTCCA TAGGAGAAGA TGACCCATTT ACAGCCAAGC TGAGCTTTGA GCAAGGTAGC 
TCTGAGTTTG TGCCTGTGGT GGTGAACCGG TCAGTACTAC GCTCCATGAG CCGAAGAGAT 
GTTCTCATCA AGCGCTGGCC CCCGCCGCTG CAATGGCAAT ATTTCCGCTC GCTGTTGCCT 
GATGCCTCCA TCACCATGTG CCCCTCCTGC TTTCAGATGT TCCATTCAGA GGACTACGAA 
CTGCTTGTGC TCCAGCACGC CTGCTGCCCT TACTGCCGAA GGCGTATCGA CGACACTGGC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ift122 ( XM_006506799.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506862.1 Mus musculus intraflagellar transport 122 (Ift122), transcript variant X3, mRNA.

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CloneID	OMu37694
Clone ID Related Accession (Same CDS sequence)	XM_006506799.4 , XM_006506799.3
Accession Version	XM_006506799.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	intraflagellar transport protein 122 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506799.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAATG GCATCATCAG TATACGGAAC AAGAATGGTG AAGAGAAAGT GAAGATCGAG 
CGCCCTGGGG GCTCCCTCTC CCCAATATGG TCCATCTGCT GGAACCCTTC AAGCCGATGG 
GAGACTATCT GGATGAGCAG AGAGAACGAG GATGCTGAGG ATGTCATTGT CAACAAATAT 
GTTCAGGAAA TCCCTTCCAT TCTGAAGTCA ACAGTGTACA ATAGTCAGGG TAGTGAGGCA 
GAGGAGGAAG AGCAAGAGGA AGAGGACGAC AGTCCCAGGG ACGGCAACTC AGAGGAACAT 
AATGACATCC TGGCTGTAGC TGACTGGGGA CAGAAACTTT CCTTCTACCA GCTGAGTGGA 
AAACAGATTG GGAAGGATCG GCCACTGAAC TTTGACCCCT GTTGCATCAG CTACTTCACT 
AAAGGAGAGT ACATTTTGGT GGGAGGTTCA GACAAGCAGG TATCTCTTTT CACCAAGGAT 
GGAGTGCGAC TGGGGACCGT TGGGGAGCAG AACTCCTGGG TGTGGACCTG TAGAGTGAAA 
CCTGACTCCA ATTATGTGGT GGTCGGCTGC CAGGATGGCA CCATCTCCTT CTACCAGCTC 
ATCTTCAGCA CAGTCCATGG GCTCTACAAA GATCGGTATG CCTACAGGGA CAGCATGACA 
GACGTCATCG TGCAGCACCT CATCACAGAG CAGAAAGTTA GGATTAAGTG CAGAGAGCTT 
GTCAAGAAAA TTGCCATCTA CAAAAATCGA TTAGCTATCC AGCTGCCAGA GAAGATCCTC 
ATCTACGAGC TGTACTCTGA AGACTCGACA GACATGCACT ACCGGGTAAA GGAGAAAATT 
GTCAAGAAGT TTGAGTGCAA CCTGCTGGTG GTGTGTGCTG ACCACATCAT CCTGTGCCAG 
GAGAAACGGC TACAGTGCCT GTCCTTCAGT GGAGTGAAGG AAAGGGAATG GCAGATGGAA 
TCTCTCATCC GCTACATCAA GGTGATCGGT GGCCCTGCTG GAAGAGAAGG TCTGTTGGTA 
GGCCTGAAAA ACGGCCAGAT CCTGAAGATC TTTGTGGACA ATCTTTTTGC TATTGTCCTG 
CTGAAGCAGG CCACAGCCGT GCGCTGCCTG GACATGAGTG CCTCCCGGAA CAAGCTGGCT 
GTGGTGGATG AGAATGACAC GTGCCTGGTG TACGACATCC ACACGAAGGA ACTGCTTTTC 
CAGGAACCGA ATGCTAACAG CGTGGCCTGG AACACGCAGT GTGAGGACAT GCTCTGCTTC 
TCTGGAGGAG GCTACCTCAA CATCAAGGCC AGCACCTTCC CAGTGCACCA GCAGAAGCTG 
CAGGGCTTTG TGGTTGGCTA CAACGGCTCC AAGATCTTCT GCCTTCATGT CTTCTCTATG 
TCTGCCGTGG AGGTGCCACA GTCCGCTCCA ATGTACCAGT ACCTCGATAG GAAGATGTTC 
AAAGAAGCAT ATCAGATCGC TTGCTTGGGA GTGACAGACG CTGATTGGCG TGAACTGGCC 
ATGGAAGCTC TAGAAGGACT AGAGTTTGAG ACGGCAAGGA AGGCCTTCAC CAGAGTCCAG 
GACCTTCGAT ACCTAGAACT GATCAGCAGT ATTGAGGAGA GGAAGAAGAG GGGAGAGACC 
AACAATGACC TGTTTCTGGC AGATGTGTTT TCCTACCAGG GGAAGTTTCA TGAAGCCGCC 
AAACTATACA AGAGGAGCGG GCATGAGAAC CTCGCTCTCG ACATGTATAC TGACCTCTGC 
ATGTTTGAGT ACGCCAAGGA TTTCCTTGGA TCTGGAGACC CTAAAGAAAC GAAGATGCTC 
ATCACCAAGC AGGCCGACTG GGCCAGAAAC ATCAATGAGC CCAAAGCTGC AGTGGAGATG 
TACATCTCAG CAGGGGAGCA CGCCAAGGCC ATAGAGATCA GCGGCAGCCA TGGCTGGGTG 
GACATGTTGA TAGACATTGC TCGGAAGCTG GACAAGGCTG AACGGGAGCC CCTGCTCATG 
TGTGCTTGCT ACTTTAAGAA GCTGGACAGT CCTGGCTATG CTGCTGAGAC CTACCTGAAG 
ATCGGGGACC TGAAGTCTCT GGTGCAGCTG TATGTGGACA CCAAGCGCTG GGATGAGGCC 
TTTGCGTTAG GTGAGAAGCA CCCGGAGTTC AAGGATGACG TCTACGTGCC CTACGCGCAG 
TGGCTGGCGG AGAACGACCG CTTTGAGGAA GCCCAGAAAG CCTTCCACAA GGCCGGGAGG 
CAGGGAGAAG CAGTCCGGGT GCTGGAGCAG CTCACGCACA ATGCTGTGGT GGAGAGCAGG 
TTTAACGACG CTGCCTATTA TTACTGGATG CTGTCCATGC AGTGCCTCGA CATGGCTCAA 
GCAGATCCTG CCCAGAAGGA CGCGATGCTG GACAAGTTTC ACCACTTCCA ACACCTGGCT 
GAGCTGTACC ATGGCTATCA AACCATTCAC CGATACACGG AGGAGCCATT CAGCTTTGAC 
CTCCCTGAAA CCCTCTTCAA CATCTCCAAG TTCTTGCTGC ACAGCCTGAC CAAGGCCACC 
CCCTTGGGTA TCTCCAAAGT GAATACACTC TTCACACTGG CTAAGCAGAG CAAGGCTCTG 
GGTGCTTACA AGCTGGCCCG GCATGCCTAT GACAAGCTAC GGGGGCTGCA GATCCCCGCC 
AGGATCCAGA AGTCCATTGA GCTGGGCACC CTGACCATCC GCTCCAAGCC CTTCCATGAC 
AGTGAGGAGC TGGTACCCTT GTGCTACCGC TGTTCTACCA ACAACCCGCT GCTGAACAAC 
CTTGGCAACG TCTGCATCAA CTGCCGCCAG CCCTTCATCT TCTCCGCCTC TTCCTATGAG 
GTGCTGCACC TGGTGGAATT CTACCTGGAA GAGGGGATCA CTGACGAAGA AGCTGTTGCC 
CTCATCGATC TGGAGGCACC AAGACACAAG AGGGAGGGCA AGTGGCGAGA GACCTCAAGC 
AACAATTCCC AGACGTTGAA GCTGGACGAG ACCATGGACT CCATAGGAGA AGATGACCCA 
TTTACAGCCA AGCTGAGCTT TGAGCAAGGT AGCTCTGAGT TTGTGCCTGT GGTGGTGAAC 
CGGTCAGTAC TACGCTCCAT GAGCCGAAGA GATGTTCTCA TCAAGCGCTG GCCCCCGCCG 
CTGCAATGGC AATATTTCCG CTCGCTGTTG CCTGATGCCT CCATCACCAT GTGCCCCTCC 
TGCTTTCAGA TGTTCCATTC AGAGGACTAC GAACTGCTTG TGCTCCAGCA CGCCTGCTGC 
CCTTACTGCC GAAGGCGTAT CGACGACACT GGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506862.1
CDS	457..3735
Translation	MANGIISIRNKNGEEKVKIERPGGSLSPIWSICWNPSSRWETIWMSRENEDAEDVIVNKYVQEIPSILKSTVYNSQGSEAEEEEQEEEDDSPRDGNSEEHNDILAVADWGQKLSFYQLSGKQIGKDRPLNFDPCCISYFTKGEYILVGGSDKQVSLFTKDGVRLGTVGEQNSWVWTCRVKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCRELVKKIAIYKNRLAIQLPEKILIYELYSEDSTDMHYRVKEKIVKKFECNLLVVCADHIILCQEKRLQCLSFSGVKEREWQMESLIRYIKVIGGPAGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRNKLAVVDENDTCLVYDIHTKELLFQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHQQKLQGFVVGYNGSKIFCLHVFSMSAVEVPQSAPMYQYLDRKMFKEAYQIACLGVTDADWRELAMEALEGLEFETARKAFTRVQDLRYLELISSIEERKKRGETNNDLFLADVFSYQGKFHEAAKLYKRSGHENLALDMYTDLCMFEYAKDFLGSGDPKETKMLITKQADWARNINEPKAAVEMYISAGEHAKAIEISGSHGWVDMLIDIARKLDKAEREPLLMCACYFKKLDSPGYAAETYLKIGDLKSLVQLYVDTKRWDEAFALGEKHPEFKDDVYVPYAQWLAENDRFEEAQKAFHKAGRQGEAVRVLEQLTHNAVVESRFNDAAYYYWMLSMQCLDMAQADPAQKDAMLDKFHHFQHLAELYHGYQTIHRYTEEPFSFDLPETLFNISKFLLHSLTKATPLGISKVNTLFTLAKQSKALGAYKLARHAYDKLRGLQIPARIQKSIELGTLTIRSKPFHDSEELVPLCYRCSTNNPLLNNLGNVCINCRQPFIFSASSYEVLHLVEFYLEEGITDEEAVALIDLEAPRHKREGKWRETSSNNSQTLKLDETMDSIGEDDPFTAKLSFEQGSSEFVPVVVNRSVLRSMSRRDVLIKRWPPPLQWQYFRSLLPDASITMCPSCFQMFHSEDYELLVLQHACCPYCRRRIDDTGP

Target ORF information:

RefSeq Version	XM_006506799.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus intraflagellar transport 122 (Ift122), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506799.3

ORF Insert Sequence:

ATGGCCAATG GCATCATCAG TATACGGAAC AAGAATGGTG AAGAGAAAGT GAAGATCGAG 
CGCCCTGGGG GCTCCCTCTC CCCAATATGG TCCATCTGCT GGAACCCTTC AAGCCGATGG 
GAGACTATCT GGATGAGCAG AGAGAACGAG GATGCTGAGG ATGTCATTGT CAACAAATAT 
GTTCAGGAAA TCCCTTCCAT TCTGAAGTCA ACAGTGTACA ATAGTCAGGG TAGTGAGGCA 
GAGGAGGAAG AGCAAGAGGA AGAGGACGAC AGTCCCAGGG ACGGCAACTC AGAGGAACAT 
AATGACATCC TGGCTGTAGC TGACTGGGGA CAGAAACTTT CCTTCTACCA GCTGAGTGGA 
AAACAGATTG GGAAGGATCG GCCACTGAAC TTTGACCCCT GTTGCATCAG CTACTTCACT 
AAAGGAGAGT ACATTTTGGT GGGAGGTTCA GACAAGCAGG TATCTCTTTT CACCAAGGAT 
GGAGTGCGAC TGGGGACCGT TGGGGAGCAG AACTCCTGGG TGTGGACCTG TAGAGTGAAA 
CCTGACTCCA ATTATGTGGT GGTCGGCTGC CAGGATGGCA CCATCTCCTT CTACCAGCTC 
ATCTTCAGCA CAGTCCATGG GCTCTACAAA GATCGGTATG CCTACAGGGA CAGCATGACA 
GACGTCATCG TGCAGCACCT CATCACAGAG CAGAAAGTTA GGATTAAGTG CAGAGAGCTT 
GTCAAGAAAA TTGCCATCTA CAAAAATCGA TTAGCTATCC AGCTGCCAGA GAAGATCCTC 
ATCTACGAGC TGTACTCTGA AGACTCGACA GACATGCACT ACCGGGTAAA GGAGAAAATT 
GTCAAGAAGT TTGAGTGCAA CCTGCTGGTG GTGTGTGCTG ACCACATCAT CCTGTGCCAG 
GAGAAACGGC TACAGTGCCT GTCCTTCAGT GGAGTGAAGG AAAGGGAATG GCAGATGGAA 
TCTCTCATCC GCTACATCAA GGTGATCGGT GGCCCTGCTG GAAGAGAAGG TCTGTTGGTA 
GGCCTGAAAA ACGGCCAGAT CCTGAAGATC TTTGTGGACA ATCTTTTTGC TATTGTCCTG 
CTGAAGCAGG CCACAGCCGT GCGCTGCCTG GACATGAGTG CCTCCCGGAA CAAGCTGGCT 
GTGGTGGATG AGAATGACAC GTGCCTGGTG TACGACATCC ACACGAAGGA ACTGCTTTTC 
CAGGAACCGA ATGCTAACAG CGTGGCCTGG AACACGCAGT GTGAGGACAT GCTCTGCTTC 
TCTGGAGGAG GCTACCTCAA CATCAAGGCC AGCACCTTCC CAGTGCACCA GCAGAAGCTG 
CAGGGCTTTG TGGTTGGCTA CAACGGCTCC AAGATCTTCT GCCTTCATGT CTTCTCTATG 
TCTGCCGTGG AGGTGCCACA GTCCGCTCCA ATGTACCAGT ACCTCGATAG GAAGATGTTC 
AAAGAAGCAT ATCAGATCGC TTGCTTGGGA GTGACAGACG CTGATTGGCG TGAACTGGCC 
ATGGAAGCTC TAGAAGGACT AGAGTTTGAG ACGGCAAGGA AGGCCTTCAC CAGAGTCCAG 
GACCTTCGAT ACCTAGAACT GATCAGCAGT ATTGAGGAGA GGAAGAAGAG GGGAGAGACC 
AACAATGACC TGTTTCTGGC AGATGTGTTT TCCTACCAGG GGAAGTTTCA TGAAGCCGCC 
AAACTATACA AGAGGAGCGG GCATGAGAAC CTCGCTCTCG ACATGTATAC TGACCTCTGC 
ATGTTTGAGT ACGCCAAGGA TTTCCTTGGA TCTGGAGACC CTAAAGAAAC GAAGATGCTC 
ATCACCAAGC AGGCCGACTG GGCCAGAAAC ATCAATGAGC CCAAAGCTGC AGTGGAGATG 
TACATCTCAG CAGGGGAGCA CGCCAAGGCC ATAGAGATCA GCGGCAGCCA TGGCTGGGTG 
GACATGTTGA TAGACATTGC TCGGAAGCTG GACAAGGCTG AACGGGAGCC CCTGCTCATG 
TGTGCTTGCT ACTTTAAGAA GCTGGACAGT CCTGGCTATG CTGCTGAGAC CTACCTGAAG 
ATCGGGGACC TGAAGTCTCT GGTGCAGCTG TATGTGGACA CCAAGCGCTG GGATGAGGCC 
TTTGCGTTAG GTGAGAAGCA CCCGGAGTTC AAGGATGACG TCTACGTGCC CTACGCGCAG 
TGGCTGGCGG AGAACGACCG CTTTGAGGAA GCCCAGAAAG CCTTCCACAA GGCCGGGAGG 
CAGGGAGAAG CAGTCCGGGT GCTGGAGCAG CTCACGCACA ATGCTGTGGT GGAGAGCAGG 
TTTAACGACG CTGCCTATTA TTACTGGATG CTGTCCATGC AGTGCCTCGA CATGGCTCAA 
GCAGATCCTG CCCAGAAGGA CGCGATGCTG GACAAGTTTC ACCACTTCCA ACACCTGGCT 
GAGCTGTACC ATGGCTATCA AACCATTCAC CGATACACGG AGGAGCCATT CAGCTTTGAC 
CTCCCTGAAA CCCTCTTCAA CATCTCCAAG TTCTTGCTGC ACAGCCTGAC CAAGGCCACC 
CCCTTGGGTA TCTCCAAAGT GAATACACTC TTCACACTGG CTAAGCAGAG CAAGGCTCTG 
GGTGCTTACA AGCTGGCCCG GCATGCCTAT GACAAGCTAC GGGGGCTGCA GATCCCCGCC 
AGGATCCAGA AGTCCATTGA GCTGGGCACC CTGACCATCC GCTCCAAGCC CTTCCATGAC 
AGTGAGGAGC TGGTACCCTT GTGCTACCGC TGTTCTACCA ACAACCCGCT GCTGAACAAC 
CTTGGCAACG TCTGCATCAA CTGCCGCCAG CCCTTCATCT TCTCCGCCTC TTCCTATGAG 
GTGCTGCACC TGGTGGAATT CTACCTGGAA GAGGGGATCA CTGACGAAGA AGCTGTTGCC 
CTCATCGATC TGGAGGCACC AAGACACAAG AGGGAGGGCA AGTGGCGAGA GACCTCAAGC 
AACAATTCCC AGACGTTGAA GCTGGACGAG ACCATGGACT CCATAGGAGA AGATGACCCA 
TTTACAGCCA AGCTGAGCTT TGAGCAAGGT AGCTCTGAGT TTGTGCCTGT GGTGGTGAAC 
CGGTCAGTAC TACGCTCCAT GAGCCGAAGA GATGTTCTCA TCAAGCGCTG GCCCCCGCCG 
CTGCAATGGC AATATTTCCG CTCGCTGTTG CCTGATGCCT CCATCACCAT GTGCCCCTCC 
TGCTTTCAGA TGTTCCATTC AGAGGACTAC GAACTGCTTG TGCTCCAGCA CGCCTGCTGC 
CCTTACTGCC GAAGGCGTAT CGACGACACT GGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proper ciliary assembly is critical for restricting Hedgehog signaling during early eye development in mice.
Developmental biology430(1)32-40(2017 10)
Burnett JB,Lupu FI,Eggenschwiler JT

ATMIN is a transcriptional regulator of both lung morphogenesis and ciliogenesis.
Development (Cambridge, England)141(20)3966-77(2014 Oct)
Goggolidou P,Stevens JL,Agueci F,Keynton J,Wheway G,Grimes DT,Patel SH,Hilton H,Morthorst SK,DiPaolo A,Williams DJ,Sanderson J,Khoronenkova SV,Powles-Glover N,Ermakov A,Esapa CT,Romero R,Dianov GL,Briscoe J,Johnson CA,Pedersen LB,Norris DP

What are those cilia doing in the neural tube?
Cilia1(1)19(2012 Oct)
Bay SN,Caspary T

The IFT-A complex regulates Shh signaling through cilia structure and membrane protein trafficking.
The Journal of cell biology197(6)789-800(2012 Jun)
Liem KF Jr,Ashe A,He M,Satir P,Moran J,Beier D,Wicking C,Anderson KV

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Ift122 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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