Egfl8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Egfl8
Entrez Gene ID	81701
Full Name	EGF-like domain 8
Synonyms	Ng3
General protein information	Preferred Names EGF-like domain 8 Names epidermal growth factor-like protein 8 EGF-like protein 8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 B1\|17 18.19 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006525179.3	XP_006525242.1	epidermal growth factor-like protein 8 isoform X1
NM_152922.3	NP_690886.3	epidermal growth factor-like protein 8 precursor
XM_006525180.3	XP_006525243.1	epidermal growth factor-like protein 8 isoform X2
XM_006525179.4	XP_006525242.1	epidermal growth factor-like protein 8 isoform X1
XM_006525180.4	XP_006525243.1	epidermal growth factor-like protein 8 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Egfl8	NP_690886.3
	EGFL8	XP_001114431.1
Bos taurus (cattle)	EGFL8	NP_001092657.1
Rattus norvegicus (Norway rat)	Egfl8	NP_001004070.1
Homo sapiens (human)	EGFL8	NP_085155.1
Pan troglodytes (chimpanzee)	EGFL8	XP_003311244.1
Xenopus tropicalis (tropical clawed frog)	egfl8	NP_001072194.1
Canis lupus familiaris (dog)	EGFL8	XP_538846.2

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Molecular characterization and expression analysis of mouse epidermal growth factor-like domain 8.
Song IJ, Ikram M, Subhan F, Choi DJ, Lee JR, Kim HS, Lim YT, Yoon S
International journal of molecular medicine36(2)541-50(2015 Aug)

Epidermal growth factor-like domain 8 inhibits the survival and proliferation of mouse thymocytes.
Subhan F, Yoon TD, Choi HJ, Muhammad I, Lee J, Hong C, Oh SO, Baek SY, Kim BS, Yoon S
International journal of molecular medicine32(4)952-8(2013 Oct)

Regulatory role of mouse epidermal growth factor-like protein 8 in thymic epithelial cells.
Choi HJ, Yoon TD, Muhammad I, Jeong MH, Lee J, Baek SY, Kim BS, Yoon S
Biochemical and biophysical research communications425(2)250-5(2012 Aug)

A mouse knockout library for secreted and transmembrane proteins.
Tang T, Li L, Tang J, Li Y, Lin WY, Martin F, Grant D, Solloway M, Parker L, Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson A, Zambrowicz BP, de Sauvage FJ
Nature biotechnology28(7)749-55(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mouse EGFL8 encodes a protein of 293 amino acids with two EGFL domains, an Emilin-like domain and a Ca(2+)-binding EGFL domain, which has a molecular mass of 32 kDa.
Title: Molecular characterization and expression analysis of mouse epidermal growth factor-like domain 8.

EGFL8 inhibited thymocyte proliferation and induced thymocyte apoptosis.
Title: Epidermal growth factor-like domain 8 inhibits the survival and proliferation of mouse thymocytes.

these data show that EGFL8 exerts inhibitory effects on thymic epithelial cells and thymocytes, suggesting that EGFL8 acts as a negative regulatory molecule in the development of T cells in the mouse thymus.
Title: Regulatory role of mouse epidermal growth factor-like protein 8 in thymic epithelial cells.

The following Egfl8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Egfl8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16176	NM_152922.3 Latest version!	Mus musculus EGF-like domain 8 (Egfl8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu16176	XM_006525179.4 Latest version!	Mus musculus EGF-like domain 8 (Egfl8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu54210	XM_006525180.4 Latest version!	Mus musculus EGF-like domain 8 (Egfl8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu16176	XM_006525179.3	Mus musculus EGF-like domain 8 (Egfl8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu54210	XM_006525180.3	Mus musculus EGF-like domain 8 (Egfl8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Egfl8 ( NM_152922.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_690886.3 Mus musculus EGF-like domain 8 (Egfl8), mRNA.

Price & Availability↑

CloneID	OMu16176
Clone ID Related Accession (Same CDS sequence)	XM_006525179.3 , NM_152922.3 , XM_006525179.4
Accession Version	NM_152922.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	epidermal growth factor-like protein 8 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK148723.1 and BC141312.1. On Apr 3, 2009 this sequence version replaced NM_152922.2. ##Evidence-Data-START## Transcript exon combination :: BC141312.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGGGCTCT GGGCTGAGCT GTGCATTTCC CTACGAGGAC TCTCATTCTT CCTGGTGCTG 
ATGACGGGCG AAGGGACCAG AGGAGGATCT TTCAAAGAGA GTTTGGGAGT GTGCTCCAAG 
CAGACGCTGC TGGTTCCTCT CCGTTACAAC GAGTCCTATA GTCAACCGGT GTACAAACCC 
TACCTGACCT TGTGTGCGGG GAGGCGCATA TGTAGCACCT ACAGGACCAC ATACCGTGTG 
GCCTGGCGGG AGGTGAGGCG GGAGGTACCA CAGACACACG TGGTGTGCTG TCAGGGCTGG 
AAGAAGCCAC ACCCAGGAGC TCTCACCTGT GATGCCATCT GCTCCAAGCC TTGTCTTAAT 
GGAGGTGTCT GCACTGGACC AGACCGGTGC GAGTGTGCCC CAGGCTGGGG AGGAAAGCAT 
TGCCACGTGG ATGTCGATGA ATGCAGGGCC AGCCTTACCC TCTGCTCTCA TGGCTGCCTC 
AACACACTGG GCAGCTTCTT GTGCAGCTGT CCACACCCCC TGGTGCTGGG TCTCGATGGA 
CGCACCTGTG CAGGGGGGCC TCCAGAGTCC CCGACAAGTG CCAGCATCCT CAGTGTGGCA 
GTTCGGGAAG CAGACAGCGA AGAGGAGCGA GCCCTGAGGT GGGAGGTTGC AGAGCTTCGT 
GGGCGTCTGG AGAAGCTGGA GCAGTGGGCC ACACAGGCTG GGGCCTGGGT TCGAGCAGTG 
CTGCCCATGC CCCCTGAAGA GCTGCGGCCT GAACAGGTGG CTGAGCTTTG GGGCCGGGGT 
GACAGAATTG AGTCCCTCAG CGACCAGGTG CTGCTACTGG AGGAAAGACT GGGTGCCTGT 
GCCTGTGAGG ATAACAGCCT GGGCCCAAGC CTGCGTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_690886.3
CDS	85..966
Translation	MGLWAELCISLRGLSFFLVLMTGEGTRGGSFKESLGVCSKQTLLVPLRYNESYSQPVYKPYLTLCAGRRICSTYRTTYRVAWREVRREVPQTHVVCCQGWKKPHPGALTCDAICSKPCLNGGVCTGPDRCECAPGWGGKHCHVDVDECRASLTLCSHGCLNTLGSFLCSCPHPLVLGLDGRTCAGGPPESPTSASILSVAVREADSEEERALRWEVAELRGRLEKLEQWATQAGAWVRAVLPMPPEELRPEQVAELWGRGDRIESLSDQVLLLEERLGACACEDNSLGPSLRG

Target ORF information:

RefSeq Version	NM_152922.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus EGF-like domain 8 (Egfl8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152922.3

ORF Insert Sequence:

ATGGGGCTCT GGGCTGAGCT GTGCATTTCC CTACGAGGAC TCTCATTCTT CCTGGTGCTG 
ATGACGGGCG AAGGGACCAG AGGAGGATCT TTCAAAGAGA GTTTGGGAGT GTGCTCCAAG 
CAGACGCTGC TGGTTCCTCT CCGTTACAAC GAGTCCTATA GTCAACCGGT GTACAAACCC 
TACCTGACCT TGTGTGCGGG GAGGCGCATA TGTAGCACCT ACAGGACCAC ATACCGTGTG 
GCCTGGCGGG AGGTGAGGCG GGAGGTACCA CAGACACACG TGGTGTGCTG TCAGGGCTGG 
AAGAAGCCAC ACCCAGGAGC TCTCACCTGT GATGCCATCT GCTCCAAGCC TTGTCTTAAT 
GGAGGTGTCT GCACTGGACC AGACCGGTGC GAGTGTGCCC CAGGCTGGGG AGGAAAGCAT 
TGCCACGTGG ATGTCGATGA ATGCAGGGCC AGCCTTACCC TCTGCTCTCA TGGCTGCCTC 
AACACACTGG GCAGCTTCTT GTGCAGCTGT CCACACCCCC TGGTGCTGGG TCTCGATGGA 
CGCACCTGTG CAGGGGGGCC TCCAGAGTCC CCGACAAGTG CCAGCATCCT CAGTGTGGCA 
GTTCGGGAAG CAGACAGCGA AGAGGAGCGA GCCCTGAGGT GGGAGGTTGC AGAGCTTCGT 
GGGCGTCTGG AGAAGCTGGA GCAGTGGGCC ACACAGGCTG GGGCCTGGGT TCGAGCAGTG 
CTGCCCATGC CCCCTGAAGA GCTGCGGCCT GAACAGGTGG CTGAGCTTTG GGGCCGGGGT 
GACAGAATTG AGTCCCTCAG CGACCAGGTG CTGCTACTGG AGGAAAGACT GGGTGCCTGT 
GCCTGTGAGG ATAACAGCCT GGGCCCAAGC CTGCGTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egfl8 ( XM_006525179.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525242.1 Mus musculus EGF-like domain 8 (Egfl8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu16176
Clone ID Related Accession (Same CDS sequence)	XM_006525179.3 , NM_152922.3 , XM_006525179.4
Accession Version	XM_006525179.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	epidermal growth factor-like protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525179.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTCT GGGCTGAGCT GTGCATTTCC CTACGAGGAC TCTCATTCTT CCTGGTGCTG 
ATGACGGGCG AAGGGACCAG AGGAGGATCT TTCAAAGAGA GTTTGGGAGT GTGCTCCAAG 
CAGACGCTGC TGGTTCCTCT CCGTTACAAC GAGTCCTATA GTCAACCGGT GTACAAACCC 
TACCTGACCT TGTGTGCGGG GAGGCGCATA TGTAGCACCT ACAGGACCAC ATACCGTGTG 
GCCTGGCGGG AGGTGAGGCG GGAGGTACCA CAGACACACG TGGTGTGCTG TCAGGGCTGG 
AAGAAGCCAC ACCCAGGAGC TCTCACCTGT GATGCCATCT GCTCCAAGCC TTGTCTTAAT 
GGAGGTGTCT GCACTGGACC AGACCGGTGC GAGTGTGCCC CAGGCTGGGG AGGAAAGCAT 
TGCCACGTGG ATGTCGATGA ATGCAGGGCC AGCCTTACCC TCTGCTCTCA TGGCTGCCTC 
AACACACTGG GCAGCTTCTT GTGCAGCTGT CCACACCCCC TGGTGCTGGG TCTCGATGGA 
CGCACCTGTG CAGGGGGGCC TCCAGAGTCC CCGACAAGTG CCAGCATCCT CAGTGTGGCA 
GTTCGGGAAG CAGACAGCGA AGAGGAGCGA GCCCTGAGGT GGGAGGTTGC AGAGCTTCGT 
GGGCGTCTGG AGAAGCTGGA GCAGTGGGCC ACACAGGCTG GGGCCTGGGT TCGAGCAGTG 
CTGCCCATGC CCCCTGAAGA GCTGCGGCCT GAACAGGTGG CTGAGCTTTG GGGCCGGGGT 
GACAGAATTG AGTCCCTCAG CGACCAGGTG CTGCTACTGG AGGAAAGACT GGGTGCCTGT 
GCCTGTGAGG ATAACAGCCT GGGCCCAAGC CTGCGTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525242.1
CDS	861..1742
Translation	MGLWAELCISLRGLSFFLVLMTGEGTRGGSFKESLGVCSKQTLLVPLRYNESYSQPVYKPYLTLCAGRRICSTYRTTYRVAWREVRREVPQTHVVCCQGWKKPHPGALTCDAICSKPCLNGGVCTGPDRCECAPGWGGKHCHVDVDECRASLTLCSHGCLNTLGSFLCSCPHPLVLGLDGRTCAGGPPESPTSASILSVAVREADSEEERALRWEVAELRGRLEKLEQWATQAGAWVRAVLPMPPEELRPEQVAELWGRGDRIESLSDQVLLLEERLGACACEDNSLGPSLRG

Target ORF information:

RefSeq Version	XM_006525179.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus EGF-like domain 8 (Egfl8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525179.3

ORF Insert Sequence:

ATGGGGCTCT GGGCTGAGCT GTGCATTTCC CTACGAGGAC TCTCATTCTT CCTGGTGCTG 
ATGACGGGCG AAGGGACCAG AGGAGGATCT TTCAAAGAGA GTTTGGGAGT GTGCTCCAAG 
CAGACGCTGC TGGTTCCTCT CCGTTACAAC GAGTCCTATA GTCAACCGGT GTACAAACCC 
TACCTGACCT TGTGTGCGGG GAGGCGCATA TGTAGCACCT ACAGGACCAC ATACCGTGTG 
GCCTGGCGGG AGGTGAGGCG GGAGGTACCA CAGACACACG TGGTGTGCTG TCAGGGCTGG 
AAGAAGCCAC ACCCAGGAGC TCTCACCTGT GATGCCATCT GCTCCAAGCC TTGTCTTAAT 
GGAGGTGTCT GCACTGGACC AGACCGGTGC GAGTGTGCCC CAGGCTGGGG AGGAAAGCAT 
TGCCACGTGG ATGTCGATGA ATGCAGGGCC AGCCTTACCC TCTGCTCTCA TGGCTGCCTC 
AACACACTGG GCAGCTTCTT GTGCAGCTGT CCACACCCCC TGGTGCTGGG TCTCGATGGA 
CGCACCTGTG CAGGGGGGCC TCCAGAGTCC CCGACAAGTG CCAGCATCCT CAGTGTGGCA 
GTTCGGGAAG CAGACAGCGA AGAGGAGCGA GCCCTGAGGT GGGAGGTTGC AGAGCTTCGT 
GGGCGTCTGG AGAAGCTGGA GCAGTGGGCC ACACAGGCTG GGGCCTGGGT TCGAGCAGTG 
CTGCCCATGC CCCCTGAAGA GCTGCGGCCT GAACAGGTGG CTGAGCTTTG GGGCCGGGGT 
GACAGAATTG AGTCCCTCAG CGACCAGGTG CTGCTACTGG AGGAAAGACT GGGTGCCTGT 
GCCTGTGAGG ATAACAGCCT GGGCCCAAGC CTGCGTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egfl8 ( XM_006525180.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525243.1 Mus musculus EGF-like domain 8 (Egfl8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu54210
Clone ID Related Accession (Same CDS sequence)	XM_006525180.3 , XM_006525180.4
Accession Version	XM_006525180.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	epidermal growth factor-like protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525180.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTCT GGGCTGAGCT GTGCATTTCC CTACGAGGAC TCTCATTCTT CCTGGTGCTG 
ATGACGGGCG AAGGGACCAG AGGAGGATCT TTCAAAGAGA GTTTGGGAGT GTGCTCCAAG 
CAGACGCTGC TGGTTCCTCT CCGTTACAAC GAGTCCTATA GTCAACCGGT GTACAAACCC 
TACCTGACCT TGTGTGCGGG GAGGCGCATA TGTAGCACCT ACAGGACCAC ATACCGTGTG 
GCCTGGCGGG AGGTGAGGCG GGAGGTACCA CAGACACACG TGGTGTGCTG TCAGGGCTGG 
AAGAAGCCAC ACCCAGGAGC TCTCACCTGT GATGCCATCT GCTCCAAGCC TTGTCTTAAT 
GGAGGTGTCT GCACTGGACC AGACCGGTGC GAGTGTGCCC CAGGCTGGGG AGGAAAGCAT 
TGCCACGTGG ATGTCGATGA ATGCAGGGCC AGCCTTACCC TCTGCTCTCA TGGCTGCCTC 
AACACACTGG GCAGCTTCTT GTGCAGCTGT CCACACCCCC TGGTGCTGGG TCTCGATGGA 
CGCACCTGTG CAGGGGGGCC TCCAGAGTCC CCGACAAGTG CCAGCATCCT CAGTGTGGCA 
GTTCGGGAAG CAGACAGCGA AGAGGAGCGA GCCCTGAGGT GGGAGGTTGC AGAGCTTCGT 
GGGCGTCTGG AGAAGCTGGA GCAGTGGGCC ACACAGGCTG GGGCCTGGGT TCGAGCAGTG 
CTGCCCATGC CCCCTGAAGA GCTGCGGCCT GAACAGGTGG CTGAGCTTTG GGGCCGGGGT 
GACAGAATTG AGTCCCTCAG CGACCAGGTG CTGCTACTGG AGGAAAGACT GGGTGCCTGT 
GAGTCCCCTG CTGCTCCTCC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525243.1
CDS	861..1727
Translation	MGLWAELCISLRGLSFFLVLMTGEGTRGGSFKESLGVCSKQTLLVPLRYNESYSQPVYKPYLTLCAGRRICSTYRTTYRVAWREVRREVPQTHVVCCQGWKKPHPGALTCDAICSKPCLNGGVCTGPDRCECAPGWGGKHCHVDVDECRASLTLCSHGCLNTLGSFLCSCPHPLVLGLDGRTCAGGPPESPTSASILSVAVREADSEEERALRWEVAELRGRLEKLEQWATQAGAWVRAVLPMPPEELRPEQVAELWGRGDRIESLSDQVLLLEERLGACESPAAPPP

Target ORF information:

RefSeq Version	XM_006525180.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus EGF-like domain 8 (Egfl8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525180.3

ORF Insert Sequence:

ATGGGGCTCT GGGCTGAGCT GTGCATTTCC CTACGAGGAC TCTCATTCTT CCTGGTGCTG 
ATGACGGGCG AAGGGACCAG AGGAGGATCT TTCAAAGAGA GTTTGGGAGT GTGCTCCAAG 
CAGACGCTGC TGGTTCCTCT CCGTTACAAC GAGTCCTATA GTCAACCGGT GTACAAACCC 
TACCTGACCT TGTGTGCGGG GAGGCGCATA TGTAGCACCT ACAGGACCAC ATACCGTGTG 
GCCTGGCGGG AGGTGAGGCG GGAGGTACCA CAGACACACG TGGTGTGCTG TCAGGGCTGG 
AAGAAGCCAC ACCCAGGAGC TCTCACCTGT GATGCCATCT GCTCCAAGCC TTGTCTTAAT 
GGAGGTGTCT GCACTGGACC AGACCGGTGC GAGTGTGCCC CAGGCTGGGG AGGAAAGCAT 
TGCCACGTGG ATGTCGATGA ATGCAGGGCC AGCCTTACCC TCTGCTCTCA TGGCTGCCTC 
AACACACTGG GCAGCTTCTT GTGCAGCTGT CCACACCCCC TGGTGCTGGG TCTCGATGGA 
CGCACCTGTG CAGGGGGGCC TCCAGAGTCC CCGACAAGTG CCAGCATCCT CAGTGTGGCA 
GTTCGGGAAG CAGACAGCGA AGAGGAGCGA GCCCTGAGGT GGGAGGTTGC AGAGCTTCGT 
GGGCGTCTGG AGAAGCTGGA GCAGTGGGCC ACACAGGCTG GGGCCTGGGT TCGAGCAGTG 
CTGCCCATGC CCCCTGAAGA GCTGCGGCCT GAACAGGTGG CTGAGCTTTG GGGCCGGGGT 
GACAGAATTG AGTCCCTCAG CGACCAGGTG CTGCTACTGG AGGAAAGACT GGGTGCCTGT 
GAGTCCCCTG CTGCTCCTCC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egfl8 ( XM_006525179.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525242.1 Mus musculus EGF-like domain 8 (Egfl8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu16176
Clone ID Related Accession (Same CDS sequence)	XM_006525179.3 , NM_152922.3 , XM_006525179.4
Accession Version	XM_006525179.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	epidermal growth factor-like protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525179.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTCT GGGCTGAGCT GTGCATTTCC CTACGAGGAC TCTCATTCTT CCTGGTGCTG 
ATGACGGGCG AAGGGACCAG AGGAGGATCT TTCAAAGAGA GTTTGGGAGT GTGCTCCAAG 
CAGACGCTGC TGGTTCCTCT CCGTTACAAC GAGTCCTATA GTCAACCGGT GTACAAACCC 
TACCTGACCT TGTGTGCGGG GAGGCGCATA TGTAGCACCT ACAGGACCAC ATACCGTGTG 
GCCTGGCGGG AGGTGAGGCG GGAGGTACCA CAGACACACG TGGTGTGCTG TCAGGGCTGG 
AAGAAGCCAC ACCCAGGAGC TCTCACCTGT GATGCCATCT GCTCCAAGCC TTGTCTTAAT 
GGAGGTGTCT GCACTGGACC AGACCGGTGC GAGTGTGCCC CAGGCTGGGG AGGAAAGCAT 
TGCCACGTGG ATGTCGATGA ATGCAGGGCC AGCCTTACCC TCTGCTCTCA TGGCTGCCTC 
AACACACTGG GCAGCTTCTT GTGCAGCTGT CCACACCCCC TGGTGCTGGG TCTCGATGGA 
CGCACCTGTG CAGGGGGGCC TCCAGAGTCC CCGACAAGTG CCAGCATCCT CAGTGTGGCA 
GTTCGGGAAG CAGACAGCGA AGAGGAGCGA GCCCTGAGGT GGGAGGTTGC AGAGCTTCGT 
GGGCGTCTGG AGAAGCTGGA GCAGTGGGCC ACACAGGCTG GGGCCTGGGT TCGAGCAGTG 
CTGCCCATGC CCCCTGAAGA GCTGCGGCCT GAACAGGTGG CTGAGCTTTG GGGCCGGGGT 
GACAGAATTG AGTCCCTCAG CGACCAGGTG CTGCTACTGG AGGAAAGACT GGGTGCCTGT 
GCCTGTGAGG ATAACAGCCT GGGCCCAAGC CTGCGTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525242.1
CDS	854..1735
Translation	MGLWAELCISLRGLSFFLVLMTGEGTRGGSFKESLGVCSKQTLLVPLRYNESYSQPVYKPYLTLCAGRRICSTYRTTYRVAWREVRREVPQTHVVCCQGWKKPHPGALTCDAICSKPCLNGGVCTGPDRCECAPGWGGKHCHVDVDECRASLTLCSHGCLNTLGSFLCSCPHPLVLGLDGRTCAGGPPESPTSASILSVAVREADSEEERALRWEVAELRGRLEKLEQWATQAGAWVRAVLPMPPEELRPEQVAELWGRGDRIESLSDQVLLLEERLGACACEDNSLGPSLRG

Target ORF information:

RefSeq Version	XM_006525179.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus EGF-like domain 8 (Egfl8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525179.4

ORF Insert Sequence:

ATGGGGCTCT GGGCTGAGCT GTGCATTTCC CTACGAGGAC TCTCATTCTT CCTGGTGCTG 
ATGACGGGCG AAGGGACCAG AGGAGGATCT TTCAAAGAGA GTTTGGGAGT GTGCTCCAAG 
CAGACGCTGC TGGTTCCTCT CCGTTACAAC GAGTCCTATA GTCAACCGGT GTACAAACCC 
TACCTGACCT TGTGTGCGGG GAGGCGCATA TGTAGCACCT ACAGGACCAC ATACCGTGTG 
GCCTGGCGGG AGGTGAGGCG GGAGGTACCA CAGACACACG TGGTGTGCTG TCAGGGCTGG 
AAGAAGCCAC ACCCAGGAGC TCTCACCTGT GATGCCATCT GCTCCAAGCC TTGTCTTAAT 
GGAGGTGTCT GCACTGGACC AGACCGGTGC GAGTGTGCCC CAGGCTGGGG AGGAAAGCAT 
TGCCACGTGG ATGTCGATGA ATGCAGGGCC AGCCTTACCC TCTGCTCTCA TGGCTGCCTC 
AACACACTGG GCAGCTTCTT GTGCAGCTGT CCACACCCCC TGGTGCTGGG TCTCGATGGA 
CGCACCTGTG CAGGGGGGCC TCCAGAGTCC CCGACAAGTG CCAGCATCCT CAGTGTGGCA 
GTTCGGGAAG CAGACAGCGA AGAGGAGCGA GCCCTGAGGT GGGAGGTTGC AGAGCTTCGT 
GGGCGTCTGG AGAAGCTGGA GCAGTGGGCC ACACAGGCTG GGGCCTGGGT TCGAGCAGTG 
CTGCCCATGC CCCCTGAAGA GCTGCGGCCT GAACAGGTGG CTGAGCTTTG GGGCCGGGGT 
GACAGAATTG AGTCCCTCAG CGACCAGGTG CTGCTACTGG AGGAAAGACT GGGTGCCTGT 
GCCTGTGAGG ATAACAGCCT GGGCCCAAGC CTGCGTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egfl8 ( XM_006525180.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525243.1 Mus musculus EGF-like domain 8 (Egfl8), transcript variant X2, mRNA.

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CloneID	OMu54210
Clone ID Related Accession (Same CDS sequence)	XM_006525180.3 , XM_006525180.4
Accession Version	XM_006525180.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	epidermal growth factor-like protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525180.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTCT GGGCTGAGCT GTGCATTTCC CTACGAGGAC TCTCATTCTT CCTGGTGCTG 
ATGACGGGCG AAGGGACCAG AGGAGGATCT TTCAAAGAGA GTTTGGGAGT GTGCTCCAAG 
CAGACGCTGC TGGTTCCTCT CCGTTACAAC GAGTCCTATA GTCAACCGGT GTACAAACCC 
TACCTGACCT TGTGTGCGGG GAGGCGCATA TGTAGCACCT ACAGGACCAC ATACCGTGTG 
GCCTGGCGGG AGGTGAGGCG GGAGGTACCA CAGACACACG TGGTGTGCTG TCAGGGCTGG 
AAGAAGCCAC ACCCAGGAGC TCTCACCTGT GATGCCATCT GCTCCAAGCC TTGTCTTAAT 
GGAGGTGTCT GCACTGGACC AGACCGGTGC GAGTGTGCCC CAGGCTGGGG AGGAAAGCAT 
TGCCACGTGG ATGTCGATGA ATGCAGGGCC AGCCTTACCC TCTGCTCTCA TGGCTGCCTC 
AACACACTGG GCAGCTTCTT GTGCAGCTGT CCACACCCCC TGGTGCTGGG TCTCGATGGA 
CGCACCTGTG CAGGGGGGCC TCCAGAGTCC CCGACAAGTG CCAGCATCCT CAGTGTGGCA 
GTTCGGGAAG CAGACAGCGA AGAGGAGCGA GCCCTGAGGT GGGAGGTTGC AGAGCTTCGT 
GGGCGTCTGG AGAAGCTGGA GCAGTGGGCC ACACAGGCTG GGGCCTGGGT TCGAGCAGTG 
CTGCCCATGC CCCCTGAAGA GCTGCGGCCT GAACAGGTGG CTGAGCTTTG GGGCCGGGGT 
GACAGAATTG AGTCCCTCAG CGACCAGGTG CTGCTACTGG AGGAAAGACT GGGTGCCTGT 
GAGTCCCCTG CTGCTCCTCC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525243.1
CDS	854..1720
Translation	MGLWAELCISLRGLSFFLVLMTGEGTRGGSFKESLGVCSKQTLLVPLRYNESYSQPVYKPYLTLCAGRRICSTYRTTYRVAWREVRREVPQTHVVCCQGWKKPHPGALTCDAICSKPCLNGGVCTGPDRCECAPGWGGKHCHVDVDECRASLTLCSHGCLNTLGSFLCSCPHPLVLGLDGRTCAGGPPESPTSASILSVAVREADSEEERALRWEVAELRGRLEKLEQWATQAGAWVRAVLPMPPEELRPEQVAELWGRGDRIESLSDQVLLLEERLGACESPAAPPP

Target ORF information:

RefSeq Version	XM_006525180.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus EGF-like domain 8 (Egfl8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525180.4

ORF Insert Sequence:

ATGGGGCTCT GGGCTGAGCT GTGCATTTCC CTACGAGGAC TCTCATTCTT CCTGGTGCTG 
ATGACGGGCG AAGGGACCAG AGGAGGATCT TTCAAAGAGA GTTTGGGAGT GTGCTCCAAG 
CAGACGCTGC TGGTTCCTCT CCGTTACAAC GAGTCCTATA GTCAACCGGT GTACAAACCC 
TACCTGACCT TGTGTGCGGG GAGGCGCATA TGTAGCACCT ACAGGACCAC ATACCGTGTG 
GCCTGGCGGG AGGTGAGGCG GGAGGTACCA CAGACACACG TGGTGTGCTG TCAGGGCTGG 
AAGAAGCCAC ACCCAGGAGC TCTCACCTGT GATGCCATCT GCTCCAAGCC TTGTCTTAAT 
GGAGGTGTCT GCACTGGACC AGACCGGTGC GAGTGTGCCC CAGGCTGGGG AGGAAAGCAT 
TGCCACGTGG ATGTCGATGA ATGCAGGGCC AGCCTTACCC TCTGCTCTCA TGGCTGCCTC 
AACACACTGG GCAGCTTCTT GTGCAGCTGT CCACACCCCC TGGTGCTGGG TCTCGATGGA 
CGCACCTGTG CAGGGGGGCC TCCAGAGTCC CCGACAAGTG CCAGCATCCT CAGTGTGGCA 
GTTCGGGAAG CAGACAGCGA AGAGGAGCGA GCCCTGAGGT GGGAGGTTGC AGAGCTTCGT 
GGGCGTCTGG AGAAGCTGGA GCAGTGGGCC ACACAGGCTG GGGCCTGGGT TCGAGCAGTG 
CTGCCCATGC CCCCTGAAGA GCTGCGGCCT GAACAGGTGG CTGAGCTTTG GGGCCGGGGT 
GACAGAATTG AGTCCCTCAG CGACCAGGTG CTGCTACTGG AGGAAAGACT GGGTGCCTGT 
GAGTCCCCTG CTGCTCCTCC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Molecular characterization and expression analysis of mouse epidermal growth factor-like domain 8.
International journal of molecular medicine36(2)541-50(2015 Aug)
Song IJ,Ikram M,Subhan F,Choi DJ,Lee JR,Kim HS,Lim YT,Yoon S

Epidermal growth factor-like domain 8 inhibits the survival and proliferation of mouse thymocytes.
International journal of molecular medicine32(4)952-8(2013 Oct)
Subhan F,Yoon TD,Choi HJ,Muhammad I,Lee J,Hong C,Oh SO,Baek SY,Kim BS,Yoon S

Regulatory role of mouse epidermal growth factor-like protein 8 in thymic epithelial cells.
Biochemical and biophysical research communications425(2)250-5(2012 Aug)
Choi HJ,Yoon TD,Muhammad I,Jeong MH,Lee J,Baek SY,Kim BS,Yoon S

A mouse knockout library for secreted and transmembrane proteins.
Nature biotechnology28(7)749-55(2010 Jul)
Tang T,Li L,Tang J,Li Y,Lin WY,Martin F,Grant D,Solloway M,Parker L,Ye W,Forrest W,Ghilardi N,Oravecz T,Platt KA,Rice DS,Hansen GM,Abuin A,Eberhart DE,Godowski P,Holt KH,Peterson A,Zambrowicz BP,de Sauvage FJ

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Egfl8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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