Dnajb1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dnajb1
Entrez Gene ID	81489
Full Name	DnaJ heat shock protein family (Hsp40) member B1
Synonyms	0610007I11Rik,DjB1,HSPF1,Hdj1,Hsp40
General protein information	Preferred Names DnaJ heat shock protein family (Hsp40) member B1 Names dnaJ homolog subfamily B member 1 DnaJ (Hsp40) homolog, subfamily B, member 1 heat shock 40 kDa protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 C2
Summary	This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. The encoded protein may also inhibit apoptosis. Peritoneal macrophages derived from homozygous knockout mice for this gene exhibit impaired heat tolerance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_018808.3	NP_061278.1	dnaJ homolog subfamily B member 1 isoform 1
NM_001308227.1	NP_001295156.1	dnaJ homolog subfamily B member 1 isoform 2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SIS1	NP_014391.1
Kluyveromyces lactis	KLLA0D04818g	XP_453274.1
Xenopus tropicalis (tropical clawed frog)	dnajb1	NP_001008112.1
Mus musculus (house mouse)	Dnajb1	NP_061278.1
Rattus norvegicus (Norway rat)	Dnajb1	NP_001101911.1
Gallus gallus (chicken)	LOC100858808	XP_003643610.2
Neurospora crassa	NCU03732	XP_961508.1
Bos taurus (cattle)	DNAJB1	NP_001028935.1
Danio rerio (zebrafish)	dnajb1a	NP_001003571.1
Eremothecium gossypii	AGOS_AAL008W	NP_982534.1
Schizosaccharomyces pombe (fission yeast)	psi1	NP_588477.1
Pan troglodytes (chimpanzee)	DNAJB1	XP_524134.4
Canis lupus familiaris (dog)	DNAJB1	XP_852900.1
Magnaporthe oryzae (rice blast fungus)	MGG_08180	XP_003715152.1
Oryza sativa (rice)	Os01g0239100	NP_001042543.1
Homo sapiens (human)	DNAJB1	NP_006136.1
Danio rerio (zebrafish)	dnajb1b	NP_956067.1

Gene Ontology
Bibliography

Related articles in PubMed

CRISPR/Cas9 Engineering of Adult Mouse Liver Demonstrates?That the Dnajb1-Prkaca Gene Fusion Is Sufficient?to Induce Tumors Resembling Fibrolamellar Hepatocellular Carcinoma.
Engelholm LH, Riaz A, Serra D, Dagn?s-Hansen F, Johansen JV, Santoni-Rugiu E, Hansen SH, Niola F, Fr?din M
Gastroenterology153(6)1662-1673.e10(2017 Dec)

Kastenhuber ER, Lalazar G, Houlihan SL, Tschaharganeh DF, Baslan T, Chen CC, Requena D, Tian S, Bosbach B, Wilkinson JE, Simon SM, Lowe SW
Proceedings of the National Academy of Sciences of the United States of America114(50)13076-13084(2017 Dec)

Overexpression of the essential Sis1 chaperone reduces TDP-43 effects on toxicity and proteolysis.
Park SK, Hong JY, Arslan F, Kanneganti V, Patel B, Tietsort A, Tank EMH, Li X, Barmada SJ, Liebman SW
PLoS genetics13(5)e1006805(2017 May)

Interaction of a Novel Chaperone PhLP2A With the Heat Shock Protein Hsp90.
Krzemie?-Ojak ?, G?ral A, Joachimiak E, Filipek A, Fabczak H
Journal of cellular biochemistry118(2)420-429(2017 Feb)

FOXE3 contributes to Peters anomaly through transcriptional regulation of an autophagy-associated protein termed DNAJB1.
Khan SY, Vasanth S, Kabir F, Gottsch JD, Khan AO, Chaerkady R, Lee MC, Leitch CC, Ma Z, Laux J, Villasmil R, Khan SN, Riazuddin S, Akram J, Cole RN, Talbot CC, Pourmand N, Zaghloul NA, Hejtmancik JF, Riazuddin SA
Nature communications710953(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Generation of the Dnajb1-Prkaca fusion gene in wild-type mice to be sufficient to initiate formation of tumors that have many features of human fibrolamellar hepatocellular carcinonma.
Title: CRISPR/Cas9 Engineering of Adult Mouse Liver Demonstrates?That the Dnajb1-Prkaca Gene Fusion Is Sufficient?to Induce Tumors Resembling Fibrolamellar Hepatocellular Carcinoma.

Overexpression of DNAJB1 relieves TDP-43 mediated toxicity in primary rodent cortical neurons.
Title: Overexpression of the essential Sis1 chaperone reduces TDP-43 effects on toxicity and proteolysis.

a biochemical link between Hsp40 expression and Abeta(42) proteostasis that is cell specific
Title: Cell line specific modulation of extracellular a?42 by Hsp40.

Hsc70, Hdj1 and Hdj2 interact with soluble and fibrillar alpha-synuclein
Title: Hsc70 protein interaction with soluble and fibrillar alpha-synuclein.

a role for Hsp40 crosstalk with CSPalpha in neuroprotection.
Title: Hsp40 couples with the CSPalpha chaperone complex upon induction of the heat shock response.

The following Dnajb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dnajb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu41827	NM_001308227.1 Latest version!	Mus musculus DnaJ heat shock protein family (Hsp40) member B1 (Dnajb1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu11040	NM_018808.3 Latest version!	Mus musculus DnaJ heat shock protein family (Hsp40) member B1 (Dnajb1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dnajb1 ( NM_001308227.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001295156.1 Mus musculus DnaJ heat shock protein family (Hsp40) member B1 (Dnajb1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu41827
Clone ID Related Accession (Same CDS sequence)	NM_001308227.1
Accession Version	NM_001308227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	dnaJ homolog subfamily B member 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC151992.3, AK158530.1, AA794374.1, BY658568.1 and BY476619.1. Transcript Variant: This variant (2) uses an alternate 5' terminal exon, resulting in a distinct 5' UTR and the use of an in-frame downstream start site, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK158530.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTGCTG AGTTCTTCGG TGGCAGAAAC CCCTTTGATA CCTTTTTTGG GCAGCGCAAC 
GGGGAAGAAG GCATGGACAT TGATGACACA TTCTCTAGCT TTCCAATGGG TATGGGTGGC 
TTCACCAACA TGAACTTTGG ACGTTCCCGC CCTTCTCAAG AGCCCACCCG GAAGAAGCAA 
GATCCTCCAG TCACCCATGA CCTTCGGGTC TCCCTTGAAG AGATCTACAG CGGCTGTACC 
AAGAAGATGA AAATCTCCCA CAAGCGGCTG AACCCTGATG GAAAGAGCAT TCGAAATGAA 
GATAAGATCC TGACCATCGA AGTGAAGAGG GGCTGGAAAG AAGGGACCAA AATCACCTTT 
CCCAAGGAAG GGGACCAGAC CTCGAACAAC ATTCCAGCAG ACATCGTCTT TGTTTTAAAG 
GACAAGCCAC ACAATATCTT CAAGAGAGAT GGTTCTGATG TCATCTATCC AGCCAGGATT 
AGCCTTCGGG AGGCTCTCTG TGGTTGCACT GTGAATGTCC CTACTCTGGA CGGCAGGACC 
ATCCCTGTTG TATTCAAAGA TGTCATCAGG CCTGGTATGC GGCGGAAAGT CCCTGGAGAA 
GGCCTCCCTC TCCCCAAAAC ACCTGAGAAA CGTGGAGACC TTGTTATCGA GTTTGAAGTG 
ATCTTCCCGG AAAGGATTCC CGTCTCATCC AGAACCATCC TGGAGCAGGT TCTTCCCATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295156.1
CDS	303..1025
Translation	MFAEFFGGRNPFDTFFGQRNGEEGMDIDDTFSSFPMGMGGFTNMNFGRSRPSQEPTRKKQDPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKRGWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLVIEFEVIFPERIPVSSRTILEQVLPI

Target ORF information:

RefSeq Version	NM_001308227.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DnaJ heat shock protein family (Hsp40) member B1 (Dnajb1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308227.1

ORF Insert Sequence:

ATGTTTGCTG AGTTCTTCGG TGGCAGAAAC CCCTTTGATA CCTTTTTTGG GCAGCGCAAC 
GGGGAAGAAG GCATGGACAT TGATGACACA TTCTCTAGCT TTCCAATGGG TATGGGTGGC 
TTCACCAACA TGAACTTTGG ACGTTCCCGC CCTTCTCAAG AGCCCACCCG GAAGAAGCAA 
GATCCTCCAG TCACCCATGA CCTTCGGGTC TCCCTTGAAG AGATCTACAG CGGCTGTACC 
AAGAAGATGA AAATCTCCCA CAAGCGGCTG AACCCTGATG GAAAGAGCAT TCGAAATGAA 
GATAAGATCC TGACCATCGA AGTGAAGAGG GGCTGGAAAG AAGGGACCAA AATCACCTTT 
CCCAAGGAAG GGGACCAGAC CTCGAACAAC ATTCCAGCAG ACATCGTCTT TGTTTTAAAG 
GACAAGCCAC ACAATATCTT CAAGAGAGAT GGTTCTGATG TCATCTATCC AGCCAGGATT 
AGCCTTCGGG AGGCTCTCTG TGGTTGCACT GTGAATGTCC CTACTCTGGA CGGCAGGACC 
ATCCCTGTTG TATTCAAAGA TGTCATCAGG CCTGGTATGC GGCGGAAAGT CCCTGGAGAA 
GGCCTCCCTC TCCCCAAAAC ACCTGAGAAA CGTGGAGACC TTGTTATCGA GTTTGAAGTG 
ATCTTCCCGG AAAGGATTCC CGTCTCATCC AGAACCATCC TGGAGCAGGT TCTTCCCATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnajb1 ( NM_018808.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_061278.1 Mus musculus DnaJ heat shock protein family (Hsp40) member B1 (Dnajb1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11040
Clone ID Related Accession (Same CDS sequence)	NM_018808.3
Accession Version	NM_018808.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	dnaJ homolog subfamily B member 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY251864.1, AK146657.1, AC151992.3, AA794374.1, BY658568.1 and BY476619.1. On Apr 28, 2015 this sequence version replaced NM_018808.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB028272.1, AK146657.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAAGG ACTACTATCA GACGCTGGGC CTGGCCCGCG GCGCGTCCGA CGACGAGATC 
AAGCGAGCCT ACCGCCGCCA GGCGCTGCGC TACCACCCGG ACAAGAACAA GGAGCCCGGC 
GCTGAGGAGA AGTTCAAGGA GATCGCCGAG GCCTACGACG TGCTCAGCGA CCCGCGCAAG 
CGCGAGATTT TCGACCGCTA TGGAGAGGAA GGCCTGAAGG GTGGTAGCCC CAGTGGAGGA 
AGCAGTGGTG GTGCTAATGG TACCTCTTTC AGCTACACAT TCCACGGAGA CCCCCATGCC 
ATGTTTGCTG AGTTCTTCGG TGGCAGAAAC CCCTTTGATA CCTTTTTTGG GCAGCGCAAC 
GGGGAAGAAG GCATGGACAT TGATGACACA TTCTCTAGCT TTCCAATGGG TATGGGTGGC 
TTCACCAACA TGAACTTTGG ACGTTCCCGC CCTTCTCAAG AGCCCACCCG GAAGAAGCAA 
GATCCTCCAG TCACCCATGA CCTTCGGGTC TCCCTTGAAG AGATCTACAG CGGCTGTACC 
AAGAAGATGA AAATCTCCCA CAAGCGGCTG AACCCTGATG GAAAGAGCAT TCGAAATGAA 
GATAAGATCC TGACCATCGA AGTGAAGAGG GGCTGGAAAG AAGGGACCAA AATCACCTTT 
CCCAAGGAAG GGGACCAGAC CTCGAACAAC ATTCCAGCAG ACATCGTCTT TGTTTTAAAG 
GACAAGCCAC ACAATATCTT CAAGAGAGAT GGTTCTGATG TCATCTATCC AGCCAGGATT 
AGCCTTCGGG AGGCTCTCTG TGGTTGCACT GTGAATGTCC CTACTCTGGA CGGCAGGACC 
ATCCCTGTTG TATTCAAAGA TGTCATCAGG CCTGGTATGC GGCGGAAAGT CCCTGGAGAA 
GGCCTCCCTC TCCCCAAAAC ACCTGAGAAA CGTGGAGACC TTGTTATCGA GTTTGAAGTG 
ATCTTCCCGG AAAGGATTCC CGTCTCATCC AGAACCATCC TGGAGCAGGT TCTTCCCATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061278.1
CDS	158..1180
Translation	MGKDYYQTLGLARGASDDEIKRAYRRQALRYHPDKNKEPGAEEKFKEIAEAYDVLSDPRKREIFDRYGEEGLKGGSPSGGSSGGANGTSFSYTFHGDPHAMFAEFFGGRNPFDTFFGQRNGEEGMDIDDTFSSFPMGMGGFTNMNFGRSRPSQEPTRKKQDPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKRGWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLVIEFEVIFPERIPVSSRTILEQVLPI

Target ORF information:

RefSeq Version	NM_018808.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DnaJ heat shock protein family (Hsp40) member B1 (Dnajb1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018808.3

ORF Insert Sequence:

ATGGGCAAGG ACTACTATCA GACGCTGGGC CTGGCCCGCG GCGCGTCCGA CGACGAGATC 
AAGCGAGCCT ACCGCCGCCA GGCGCTGCGC TACCACCCGG ACAAGAACAA GGAGCCCGGC 
GCTGAGGAGA AGTTCAAGGA GATCGCCGAG GCCTACGACG TGCTCAGCGA CCCGCGCAAG 
CGCGAGATTT TCGACCGCTA TGGAGAGGAA GGCCTGAAGG GTGGTAGCCC CAGTGGAGGA 
AGCAGTGGTG GTGCTAATGG TACCTCTTTC AGCTACACAT TCCACGGAGA CCCCCATGCC 
ATGTTTGCTG AGTTCTTCGG TGGCAGAAAC CCCTTTGATA CCTTTTTTGG GCAGCGCAAC 
GGGGAAGAAG GCATGGACAT TGATGACACA TTCTCTAGCT TTCCAATGGG TATGGGTGGC 
TTCACCAACA TGAACTTTGG ACGTTCCCGC CCTTCTCAAG AGCCCACCCG GAAGAAGCAA 
GATCCTCCAG TCACCCATGA CCTTCGGGTC TCCCTTGAAG AGATCTACAG CGGCTGTACC 
AAGAAGATGA AAATCTCCCA CAAGCGGCTG AACCCTGATG GAAAGAGCAT TCGAAATGAA 
GATAAGATCC TGACCATCGA AGTGAAGAGG GGCTGGAAAG AAGGGACCAA AATCACCTTT 
CCCAAGGAAG GGGACCAGAC CTCGAACAAC ATTCCAGCAG ACATCGTCTT TGTTTTAAAG 
GACAAGCCAC ACAATATCTT CAAGAGAGAT GGTTCTGATG TCATCTATCC AGCCAGGATT 
AGCCTTCGGG AGGCTCTCTG TGGTTGCACT GTGAATGTCC CTACTCTGGA CGGCAGGACC 
ATCCCTGTTG TATTCAAAGA TGTCATCAGG CCTGGTATGC GGCGGAAAGT CCCTGGAGAA 
GGCCTCCCTC TCCCCAAAAC ACCTGAGAAA CGTGGAGACC TTGTTATCGA GTTTGAAGTG 
ATCTTCCCGG AAAGGATTCC CGTCTCATCC AGAACCATCC TGGAGCAGGT TCTTCCCATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CRISPR/Cas9 Engineering of Adult Mouse Liver Demonstrates?That the Dnajb1-Prkaca Gene Fusion Is Sufficient?to Induce Tumors Resembling Fibrolamellar Hepatocellular Carcinoma.
Gastroenterology153(6)1662-1673.e10(2017 Dec)
Engelholm LH,Riaz A,Serra D,Dagn?s-Hansen F,Johansen JV,Santoni-Rugiu E,Hansen SH,Niola F,Fr?din M

Proceedings of the National Academy of Sciences of the United States of America114(50)13076-13084(2017 Dec)
Kastenhuber ER,Lalazar G,Houlihan SL,Tschaharganeh DF,Baslan T,Chen CC,Requena D,Tian S,Bosbach B,Wilkinson JE,Simon SM,Lowe SW

Overexpression of the essential Sis1 chaperone reduces TDP-43 effects on toxicity and proteolysis.
PLoS genetics13(5)e1006805(2017 May)
Park SK,Hong JY,Arslan F,Kanneganti V,Patel B,Tietsort A,Tank EMH,Li X,Barmada SJ,Liebman SW

Interaction of a Novel Chaperone PhLP2A With the Heat Shock Protein Hsp90.
Journal of cellular biochemistry118(2)420-429(2017 Feb)
Krzemie?-Ojak ?,G?ral A,Joachimiak E,Filipek A,Fabczak H

FOXE3 contributes to Peters anomaly through transcriptional regulation of an autophagy-associated protein termed DNAJB1.
Nature communications710953(2016 Apr)
Khan SY,Vasanth S,Kabir F,Gottsch JD,Khan AO,Chaerkady R,Lee MC,Leitch CC,Ma Z,Laux J,Villasmil R,Khan SN,Riazuddin S,Akram J,Cole RN,Talbot CC,Pourmand N,Zaghloul NA,Hejtmancik JF,Riazuddin SA

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Dnajb1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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