Rnf114 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rnf114
Entrez Gene ID	81018
Full Name	ring finger protein 114
Synonyms	1110008J21Rik,AI225886,AW549494,Zfp228,Zfp313,Znf228
General protein information	Preferred Names ring finger protein 114 Names E3 ubiquitin-protein ligase RNF114 RING-type E3 ubiquitin transferase RNF114 zinc finger protein 313
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H3

mRNA and Protein(s)

mRNA	Protein	Name
NM_030743.5	NP_109668.2	E3 ubiquitin-protein ligase RNF114 isoform 1
NM_030743.6	NP_109668.2	E3 ubiquitin-protein ligase RNF114 isoform 1
NM_001360930.1	NP_001347859.1	E3 ubiquitin-protein ligase RNF114 isoform 1
NM_001356496.1	NP_001343425.1	E3 ubiquitin-protein ligase RNF114 isoform 2
XM_006500439.1	XP_006500502.1	E3 ubiquitin-protein ligase RNF114 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RNF114	NP_001019702.1
Pan troglodytes (chimpanzee)	RNF114	NP_001120978.1
Danio rerio (zebrafish)	rnf114	NP_001001828.2
Canis lupus familiaris (dog)	RNF114	XP_534453.3
Mus musculus (house mouse)	Rnf114	NP_109668.2
Rattus norvegicus (Norway rat)	Rnf114	NP_001001517.1
Homo sapiens (human)	RNF114	NP_061153.1
Gallus gallus (chicken)	RNF114	NP_001001767.2
Xenopus tropicalis (tropical clawed frog)	rnf114	NP_001001857.1

Gene Ontology
Bibliography

Related articles in PubMed

Negative regulation of the RLH signaling by the E3 ubiquitin ligase RNF114.
Lin B, Ke Q, Li H, Pheifer NS, Velliquette DC, Leaman DW
Cytokine99186-193(2017 Nov)

The E3 ubiquitin ligase RNF114 and TAB1 degradation are required for maternal-to-zygotic transition.
Yang Y, Zhou C, Wang Y, Liu W, Liu C, Wang L, Liu Y, Shang Y, Li M, Zhou S, Wang Y, Zeng W, Zhou J, Huo R, Li W
EMBO reports18(2)205-216(2017 Feb)

ZNF313 is a novel cell cycle activator with an E3 ligase activity inhibiting cellular senescence by destabilizing p21(WAF1.).
Han J, Kim YL, Lee KW, Her NG, Ha TK, Yoon S, Jeong SI, Lee JH, Kang MJ, Lee MG, Ryu BK, Baik JH, Chi SG
Cell death and differentiation20(8)1055-67(2013 Aug)

Deterministic and stochastic allele specific gene expression in single mouse blastomeres.
Tang F, Barbacioru C, Nordman E, Bao S, Lee C, Wang X, Tuch BB, Heard E, Lao K, Surani MA
PloS one6(6)e21208(2011)

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Guo G, Huss M, Tong GQ, Wang C, Li Sun L, Clarke ND, Robson P
Developmental cell18(4)675-85(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our study uncovers that RNF114-mediated ubiquitination and degradation of TAB1 activate the NF-kappaB pathway during MZT, and thus directly link maternal clearance to early embryo development.
Title: The E3 ubiquitin ligase RNF114 and TAB1 degradation are required for maternal-to-zygotic transition.

ZNF313 is a novel cell cycle activator with an E3 ligase activity inhibiting cellular senescence by destabilizing p21(WAF1.).
Title: ZNF313 is a novel cell cycle activator with an E3 ligase activity inhibiting cellular senescence by destabilizing p21(WAF1.).

Present data suggests that Znf313 gene might play a fundamental role in gene transcription and regulation and relates to spermatogenesis.
Title: Cloning and expression analysis of a novel mouse zinc finger protein gene Znf313 abundantly expressed in testis.

The following Rnf114 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rnf114 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18589	NM_001360930.1 Latest version!	Mus musculus ring finger protein 114 (Rnf114), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu18589	NM_030743.6 Latest version!	Mus musculus ring finger protein 114 (Rnf114), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu83843	NM_001356496.1 Latest version!	Mus musculus ring finger protein 114 (Rnf114), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu18589	XM_006500439.1 Latest version!	Mus musculus ring finger protein 114 (Rnf114), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
View Old Accession Versions >>
OMu18589	NM_030743.5	Mus musculus ring finger protein 114 (Rnf114), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rnf114 ( NM_030743.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_109668.2 Mus musculus ring finger protein 114 (Rnf114), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18589
Clone ID Related Accession (Same CDS sequence)	NM_030743.5 , NM_030743.6 , NM_001360930.1 , XM_006500439.1
Accession Version	NM_030743.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-04
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF114 isoform 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL589870.30. On Mar 21, 2007 this sequence version replaced NM_030743.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC085146.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01164135 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CGCAGCCGGA GTCCCGAGAT GGAGCCGCGC AGTCGGCGAA GCCGGCGTCC 
GAGACGGACC CCCTCAGCCG CTTTACGTGT CCCGTGTGCC TGGAAGTATT CGAGAAGCCG 
GTGCAGGTGC CCTGCGGACA CGTCTTTTGC TCTGCATGCC TGCAGGAATG TCTGAAGCCG 
AAGAAACCTG TCTGTGGGGT GTGTCGCAGC GCTCTGGCAC CTGGCGTCCG AGCTGTGGAG 
CTCGAGCGGC AGATCGAGAG CATAGAGACT TCTTGCCATG GCTGCCGTAA GAATTTCATC 
CTATCTAAGA TCCGGGCCCA TGTGACGTCA TGTTCCAAAT ACCAGAATTA CATCATGGAA 
GGTGTAAAGG CCACCACCAA GGATGCATCC CTTCAGCCGA GAAACATCCC AAACCGATAC 
ACCTTTCCTT GTCCTTACTG TCCCGAGAAG AACTTTGATC AGGAAGGACT CGTGGAGCAC 
TGCAAACTCA CTCACAGCAC CGATACCAAG TCTGTGGTTT GTCCCATCTG TGCCTCGATG 
CCCTGGGGAG ACCCCAGCTA CCGCAGTGCC AACTTCATGG AGCATATCCA ACGCCGTCAC 
CGCTTTTCCT ACGACACTTT TGTGGATTAC GATGTTGATG AAGATGACAT GATAAACCAG 
GTGTTGCAGC GCTCCATCAT TGACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_109668.2
CDS	877..1566
Translation	MAAAQPESRDGAAQSAKPASETDPLSRFTCPVCLEVFEKPVQVPCGHVFCSACLQECLKPKKPVCGVCRSALAPGVRAVELERQIESIETSCHGCRKNFILSKIRAHVTSCSKYQNYIMEGVKATTKDASLQPRNIPNRYTFPCPYCPEKNFDQEGLVEHCKLTHSTDTKSVVCPICASMPWGDPSYRSANFMEHIQRRHRFSYDTFVDYDVDEDDMINQVLQRSIIDQ

Target ORF information:

RefSeq Version	NM_030743.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 114 (Rnf114), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030743.5

ORF Insert Sequence:

ATGGCGGCGG CGCAGCCGGA GTCCCGAGAT GGAGCCGCGC AGTCGGCGAA GCCGGCGTCC 
GAGACGGACC CCCTCAGCCG CTTTACGTGT CCCGTGTGCC TGGAAGTATT CGAGAAGCCG 
GTGCAGGTGC CCTGCGGACA CGTCTTTTGC TCTGCATGCC TGCAGGAATG TCTGAAGCCG 
AAGAAACCTG TCTGTGGGGT GTGTCGCAGC GCTCTGGCAC CTGGCGTCCG AGCTGTGGAG 
CTCGAGCGGC AGATCGAGAG CATAGAGACT TCTTGCCATG GCTGCCGTAA GAATTTCATC 
CTATCTAAGA TCCGGGCCCA TGTGACGTCA TGTTCCAAAT ACCAGAATTA CATCATGGAA 
GGTGTAAAGG CCACCACCAA GGATGCATCC CTTCAGCCGA GAAACATCCC AAACCGATAC 
ACCTTTCCTT GTCCTTACTG TCCCGAGAAG AACTTTGATC AGGAAGGACT CGTGGAGCAC 
TGCAAACTCA CTCACAGCAC CGATACCAAG TCTGTGGTTT GTCCCATCTG TGCCTCGATG 
CCCTGGGGAG ACCCCAGCTA CCGCAGTGCC AACTTCATGG AGCATATCCA ACGCCGTCAC 
CGCTTTTCCT ACGACACTTT TGTGGATTAC GATGTTGATG AAGATGACAT GATAAACCAG 
GTGTTGCAGC GCTCCATCAT TGACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf114 ( NM_030743.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_109668.2 Mus musculus ring finger protein 114 (Rnf114), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18589
Clone ID Related Accession (Same CDS sequence)	NM_030743.5 , NM_030743.6 , NM_001360930.1 , XM_006500439.1
Accession Version	NM_030743.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF114 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL589870.30. On Feb 17, 2018 this sequence version replaced NM_030743.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 3 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC085146.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01164135 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CGCAGCCGGA GTCCCGAGAT GGAGCCGCGC AGTCGGCGAA GCCGGCGTCC 
GAGACGGACC CCCTCAGCCG CTTTACGTGT CCCGTGTGCC TGGAAGTATT CGAGAAGCCG 
GTGCAGGTGC CCTGCGGACA CGTCTTTTGC TCTGCATGCC TGCAGGAATG TCTGAAGCCG 
AAGAAACCTG TCTGTGGGGT GTGTCGCAGC GCTCTGGCAC CTGGCGTCCG AGCTGTGGAG 
CTCGAGCGGC AGATCGAGAG CATAGAGACT TCTTGCCATG GCTGCCGTAA GAATTTCATC 
CTATCTAAGA TCCGGGCCCA TGTGACGTCA TGTTCCAAAT ACCAGAATTA CATCATGGAA 
GGTGTAAAGG CCACCACCAA GGATGCATCC CTTCAGCCGA GAAACATCCC AAACCGATAC 
ACCTTTCCTT GTCCTTACTG TCCCGAGAAG AACTTTGATC AGGAAGGACT CGTGGAGCAC 
TGCAAACTCA CTCACAGCAC CGATACCAAG TCTGTGGTTT GTCCCATCTG TGCCTCGATG 
CCCTGGGGAG ACCCCAGCTA CCGCAGTGCC AACTTCATGG AGCATATCCA ACGCCGTCAC 
CGCTTTTCCT ACGACACTTT TGTGGATTAC GATGTTGATG AAGATGACAT GATAAACCAG 
GTGTTGCAGC GCTCCATCAT TGACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_109668.2
CDS	877..1566
Translation	MAAAQPESRDGAAQSAKPASETDPLSRFTCPVCLEVFEKPVQVPCGHVFCSACLQECLKPKKPVCGVCRSALAPGVRAVELERQIESIETSCHGCRKNFILSKIRAHVTSCSKYQNYIMEGVKATTKDASLQPRNIPNRYTFPCPYCPEKNFDQEGLVEHCKLTHSTDTKSVVCPICASMPWGDPSYRSANFMEHIQRRHRFSYDTFVDYDVDEDDMINQVLQRSIIDQ

Target ORF information:

RefSeq Version	NM_030743.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 114 (Rnf114), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030743.6

ORF Insert Sequence:

ATGGCGGCGG CGCAGCCGGA GTCCCGAGAT GGAGCCGCGC AGTCGGCGAA GCCGGCGTCC 
GAGACGGACC CCCTCAGCCG CTTTACGTGT CCCGTGTGCC TGGAAGTATT CGAGAAGCCG 
GTGCAGGTGC CCTGCGGACA CGTCTTTTGC TCTGCATGCC TGCAGGAATG TCTGAAGCCG 
AAGAAACCTG TCTGTGGGGT GTGTCGCAGC GCTCTGGCAC CTGGCGTCCG AGCTGTGGAG 
CTCGAGCGGC AGATCGAGAG CATAGAGACT TCTTGCCATG GCTGCCGTAA GAATTTCATC 
CTATCTAAGA TCCGGGCCCA TGTGACGTCA TGTTCCAAAT ACCAGAATTA CATCATGGAA 
GGTGTAAAGG CCACCACCAA GGATGCATCC CTTCAGCCGA GAAACATCCC AAACCGATAC 
ACCTTTCCTT GTCCTTACTG TCCCGAGAAG AACTTTGATC AGGAAGGACT CGTGGAGCAC 
TGCAAACTCA CTCACAGCAC CGATACCAAG TCTGTGGTTT GTCCCATCTG TGCCTCGATG 
CCCTGGGGAG ACCCCAGCTA CCGCAGTGCC AACTTCATGG AGCATATCCA ACGCCGTCAC 
CGCTTTTCCT ACGACACTTT TGTGGATTAC GATGTTGATG AAGATGACAT GATAAACCAG 
GTGTTGCAGC GCTCCATCAT TGACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf114 ( NM_001360930.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347859.1 Mus musculus ring finger protein 114 (Rnf114), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu18589
Clone ID Related Accession (Same CDS sequence)	NM_030743.5 , NM_030743.6 , NM_001360930.1 , XM_006500439.1
Accession Version	NM_001360930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF114 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK148290.1 and AF502145.1. On Feb 17, 2018 this sequence version replaced XM_006500439.1. ##Evidence-Data-START## Transcript exon combination :: AF282919.2, AF502145.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CGCAGCCGGA GTCCCGAGAT GGAGCCGCGC AGTCGGCGAA GCCGGCGTCC 
GAGACGGACC CCCTCAGCCG CTTTACGTGT CCCGTGTGCC TGGAAGTATT CGAGAAGCCG 
GTGCAGGTGC CCTGCGGACA CGTCTTTTGC TCTGCATGCC TGCAGGAATG TCTGAAGCCG 
AAGAAACCTG TCTGTGGGGT GTGTCGCAGC GCTCTGGCAC CTGGCGTCCG AGCTGTGGAG 
CTCGAGCGGC AGATCGAGAG CATAGAGACT TCTTGCCATG GCTGCCGTAA GAATTTCATC 
CTATCTAAGA TCCGGGCCCA TGTGACGTCA TGTTCCAAAT ACCAGAATTA CATCATGGAA 
GGTGTAAAGG CCACCACCAA GGATGCATCC CTTCAGCCGA GAAACATCCC AAACCGATAC 
ACCTTTCCTT GTCCTTACTG TCCCGAGAAG AACTTTGATC AGGAAGGACT CGTGGAGCAC 
TGCAAACTCA CTCACAGCAC CGATACCAAG TCTGTGGTTT GTCCCATCTG TGCCTCGATG 
CCCTGGGGAG ACCCCAGCTA CCGCAGTGCC AACTTCATGG AGCATATCCA ACGCCGTCAC 
CGCTTTTCCT ACGACACTTT TGTGGATTAC GATGTTGATG AAGATGACAT GATAAACCAG 
GTGTTGCAGC GCTCCATCAT TGACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347859.1
CDS	17..706
Translation	MAAAQPESRDGAAQSAKPASETDPLSRFTCPVCLEVFEKPVQVPCGHVFCSACLQECLKPKKPVCGVCRSALAPGVRAVELERQIESIETSCHGCRKNFILSKIRAHVTSCSKYQNYIMEGVKATTKDASLQPRNIPNRYTFPCPYCPEKNFDQEGLVEHCKLTHSTDTKSVVCPICASMPWGDPSYRSANFMEHIQRRHRFSYDTFVDYDVDEDDMINQVLQRSIIDQ

Target ORF information:

RefSeq Version	NM_001360930.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 114 (Rnf114), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360930.1

ORF Insert Sequence:

ATGGCGGCGG CGCAGCCGGA GTCCCGAGAT GGAGCCGCGC AGTCGGCGAA GCCGGCGTCC 
GAGACGGACC CCCTCAGCCG CTTTACGTGT CCCGTGTGCC TGGAAGTATT CGAGAAGCCG 
GTGCAGGTGC CCTGCGGACA CGTCTTTTGC TCTGCATGCC TGCAGGAATG TCTGAAGCCG 
AAGAAACCTG TCTGTGGGGT GTGTCGCAGC GCTCTGGCAC CTGGCGTCCG AGCTGTGGAG 
CTCGAGCGGC AGATCGAGAG CATAGAGACT TCTTGCCATG GCTGCCGTAA GAATTTCATC 
CTATCTAAGA TCCGGGCCCA TGTGACGTCA TGTTCCAAAT ACCAGAATTA CATCATGGAA 
GGTGTAAAGG CCACCACCAA GGATGCATCC CTTCAGCCGA GAAACATCCC AAACCGATAC 
ACCTTTCCTT GTCCTTACTG TCCCGAGAAG AACTTTGATC AGGAAGGACT CGTGGAGCAC 
TGCAAACTCA CTCACAGCAC CGATACCAAG TCTGTGGTTT GTCCCATCTG TGCCTCGATG 
CCCTGGGGAG ACCCCAGCTA CCGCAGTGCC AACTTCATGG AGCATATCCA ACGCCGTCAC 
CGCTTTTCCT ACGACACTTT TGTGGATTAC GATGTTGATG AAGATGACAT GATAAACCAG 
GTGTTGCAGC GCTCCATCAT TGACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf114 ( NM_001356496.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001343425.1 Mus musculus ring finger protein 114 (Rnf114), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu83843
Clone ID Related Accession (Same CDS sequence)	NM_001356496.1
Accession Version	NM_001356496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-04
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF114 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL589870.30. Transcript Variant: This variant (2) lacks an alternate exon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01164135 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGTGC TAGGAGCCCA ACTCAGGTCG TCTAGAAGAG CACGCGACGC TCCTCAGCGG 
CAAGATGGCG GCGGCGCAGC CGGAGTCCCG AGATGGAGCC GCGCAGTCGG CGAAGCCGGC 
GTCCGAGACG GACCCCCTCA GCCGCTTTAC GTGTCCCGTG TGCCTGGAAG TATTCGAGAA 
GCCGGTGCAG GTGCCCTGCG GACACGTTTC ATCCTATCTA AGATCCGGGC CCATGTGACG 
TCATGTTCCA AATACCAGAA TTACATCATG GAAGGTGTAA AGGCCACCAC CAAGGATGCA 
TCCCTTCAGC CGAGAAACAT CCCAAACCGA TACACCTTTC CTTGTCCTTA CTGTCCCGAG 
AAGAACTTTG ATCAGGAAGG ACTCGTGGAG CACTGCAAAC TCACTCACAG CACCGATACC 
AAGTCTGTGG TTTGTCCCAT CTGTGCCTCG ATGCCCTGGG GAGACCCCAG CTACCGCAGT 
GCCAACTTCA TGGAGCATAT CCAACGCCGT CACCGCTTTT CCTACGACAC TTTTGTGGAT 
TACGATGTTG ATGAAGATGA CATGATAAAC CAGGTGTTGC AGCGCTCCAT CATTGACCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343425.1
CDS	813..1415
Translation	MWVLGAQLRSSRRARDAPQRQDGGGAAGVPRWSRAVGEAGVRDGPPQPLYVSRVPGSIREAGAGALRTRFILSKIRAHVTSCSKYQNYIMEGVKATTKDASLQPRNIPNRYTFPCPYCPEKNFDQEGLVEHCKLTHSTDTKSVVCPICASMPWGDPSYRSANFMEHIQRRHRFSYDTFVDYDVDEDDMINQVLQRSIIDQ

Target ORF information:

RefSeq Version	NM_001356496.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 114 (Rnf114), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356496.1

ORF Insert Sequence:

ATGTGGGTGC TAGGAGCCCA ACTCAGGTCG TCTAGAAGAG CACGCGACGC TCCTCAGCGG 
CAAGATGGCG GCGGCGCAGC CGGAGTCCCG AGATGGAGCC GCGCAGTCGG CGAAGCCGGC 
GTCCGAGACG GACCCCCTCA GCCGCTTTAC GTGTCCCGTG TGCCTGGAAG TATTCGAGAA 
GCCGGTGCAG GTGCCCTGCG GACACGTTTC ATCCTATCTA AGATCCGGGC CCATGTGACG 
TCATGTTCCA AATACCAGAA TTACATCATG GAAGGTGTAA AGGCCACCAC CAAGGATGCA 
TCCCTTCAGC CGAGAAACAT CCCAAACCGA TACACCTTTC CTTGTCCTTA CTGTCCCGAG 
AAGAACTTTG ATCAGGAAGG ACTCGTGGAG CACTGCAAAC TCACTCACAG CACCGATACC 
AAGTCTGTGG TTTGTCCCAT CTGTGCCTCG ATGCCCTGGG GAGACCCCAG CTACCGCAGT 
GCCAACTTCA TGGAGCATAT CCAACGCCGT CACCGCTTTT CCTACGACAC TTTTGTGGAT 
TACGATGTTG ATGAAGATGA CATGATAAAC CAGGTGTTGC AGCGCTCCAT CATTGACCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf114 ( XM_006500439.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500502.1 Mus musculus ring finger protein 114 (Rnf114), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu18589
Clone ID Related Accession (Same CDS sequence)	NM_030743.5 , NM_030743.6 , NM_001360930.1 , XM_006500439.1
Accession Version	XM_006500439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF114 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGCAGCCGGA GTCCCGAGAT GGAGCCGCGC AGTCGGCGAA GCCGGCGTCC 
GAGACGGACC CCCTCAGCCG CTTTACGTGT CCCGTGTGCC TGGAAGTATT CGAGAAGCCG 
GTGCAGGTGC CCTGCGGACA CGTCTTTTGC TCTGCATGCC TGCAGGAATG TCTGAAGCCG 
AAGAAACCTG TCTGTGGGGT GTGTCGCAGC GCTCTGGCAC CTGGCGTCCG AGCTGTGGAG 
CTCGAGCGGC AGATCGAGAG CATAGAGACT TCTTGCCATG GCTGCCGTAA GAATTTCATC 
CTATCTAAGA TCCGGGCCCA TGTGACGTCA TGTTCCAAAT ACCAGAATTA CATCATGGAA 
GGTGTAAAGG CCACCACCAA GGATGCATCC CTTCAGCCGA GAAACATCCC AAACCGATAC 
ACCTTTCCTT GTCCTTACTG TCCCGAGAAG AACTTTGATC AGGAAGGACT CGTGGAGCAC 
TGCAAACTCA CTCACAGCAC CGATACCAAG TCTGTGGTTT GTCCCATCTG TGCCTCGATG 
CCCTGGGGAG ACCCCAGCTA CCGCAGTGCC AACTTCATGG AGCATATCCA ACGCCGTCAC 
CGCTTTTCCT ACGACACTTT TGTGGATTAC GATGTTGATG AAGATGACAT GATAAACCAG 
GTGTTGCAGC GCTCCATCAT TGACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500502.1
CDS	366..1055
Translation	MAAAQPESRDGAAQSAKPASETDPLSRFTCPVCLEVFEKPVQVPCGHVFCSACLQECLKPKKPVCGVCRSALAPGVRAVELERQIESIETSCHGCRKNFILSKIRAHVTSCSKYQNYIMEGVKATTKDASLQPRNIPNRYTFPCPYCPEKNFDQEGLVEHCKLTHSTDTKSVVCPICASMPWGDPSYRSANFMEHIQRRHRFSYDTFVDYDVDEDDMINQVLQRSIIDQ

Target ORF information:

RefSeq Version	XM_006500439.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 114 (Rnf114), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500439.1

ORF Insert Sequence:

ATGGCGGCGG CGCAGCCGGA GTCCCGAGAT GGAGCCGCGC AGTCGGCGAA GCCGGCGTCC 
GAGACGGACC CCCTCAGCCG CTTTACGTGT CCCGTGTGCC TGGAAGTATT CGAGAAGCCG 
GTGCAGGTGC CCTGCGGACA CGTCTTTTGC TCTGCATGCC TGCAGGAATG TCTGAAGCCG 
AAGAAACCTG TCTGTGGGGT GTGTCGCAGC GCTCTGGCAC CTGGCGTCCG AGCTGTGGAG 
CTCGAGCGGC AGATCGAGAG CATAGAGACT TCTTGCCATG GCTGCCGTAA GAATTTCATC 
CTATCTAAGA TCCGGGCCCA TGTGACGTCA TGTTCCAAAT ACCAGAATTA CATCATGGAA 
GGTGTAAAGG CCACCACCAA GGATGCATCC CTTCAGCCGA GAAACATCCC AAACCGATAC 
ACCTTTCCTT GTCCTTACTG TCCCGAGAAG AACTTTGATC AGGAAGGACT CGTGGAGCAC 
TGCAAACTCA CTCACAGCAC CGATACCAAG TCTGTGGTTT GTCCCATCTG TGCCTCGATG 
CCCTGGGGAG ACCCCAGCTA CCGCAGTGCC AACTTCATGG AGCATATCCA ACGCCGTCAC 
CGCTTTTCCT ACGACACTTT TGTGGATTAC GATGTTGATG AAGATGACAT GATAAACCAG 
GTGTTGCAGC GCTCCATCAT TGACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Negative regulation of the RLH signaling by the E3 ubiquitin ligase RNF114.
Cytokine99186-193(2017 Nov)
Lin B,Ke Q,Li H,Pheifer NS,Velliquette DC,Leaman DW

The E3 ubiquitin ligase RNF114 and TAB1 degradation are required for maternal-to-zygotic transition.
EMBO reports18(2)205-216(2017 Feb)
Yang Y,Zhou C,Wang Y,Liu W,Liu C,Wang L,Liu Y,Shang Y,Li M,Zhou S,Wang Y,Zeng W,Zhou J,Huo R,Li W

ZNF313 is a novel cell cycle activator with an E3 ligase activity inhibiting cellular senescence by destabilizing p21(WAF1.).
Cell death and differentiation20(8)1055-67(2013 Aug)
Han J,Kim YL,Lee KW,Her NG,Ha TK,Yoon S,Jeong SI,Lee JH,Kang MJ,Lee MG,Ryu BK,Baik JH,Chi SG

Deterministic and stochastic allele specific gene expression in single mouse blastomeres.
PloS one6(6)e21208(2011)
Tang F,Barbacioru C,Nordman E,Bao S,Lee C,Wang X,Tuch BB,Heard E,Lao K,Surani MA

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Developmental cell18(4)675-85(2010 Apr)
Guo G,Huss M,Tong GQ,Wang C,Li Sun L,Clarke ND,Robson P

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Rnf114 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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