Erap1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Erap1
Entrez Gene ID	80898
Full Name	endoplasmic reticulum aminopeptidase 1
Synonyms	Arts1,ERAAP,PILSA,PILSAP
General protein information	Preferred Names endoplasmic reticulum aminopeptidase 1 Names endoplasmic reticulum aminopeptidase 1 A-LAP ARTS-1 PILS-AP VEGF-induced aminopeptidase adipocyte-derived leucine aminopeptidase aminopeptidase PILS puromycin-insensitive leucyl-specific aminopeptidase type 1 tumor necrosis factor receptor shedding aminopeptidase regulator
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_030711.5	NP_109636.1	endoplasmic reticulum aminopeptidase 1 precursor
XM_030247471.1	XP_030103331.1	endoplasmic reticulum aminopeptidase 1 isoform X2
XM_006517476.3	XP_006517539.1	endoplasmic reticulum aminopeptidase 1 isoform X1
XM_006517476.4	XP_006517539.1	endoplasmic reticulum aminopeptidase 1 isoform X1
NM_030711.4	NP_109636.1	endoplasmic reticulum aminopeptidase 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ERAP1	XP_003310814.1
Mus musculus (house mouse)	Erap1	NP_109636.1
Danio rerio (zebrafish)	zgc:56194	NP_956500.2
	ERAP1	XP_001094790.1
Danio rerio (zebrafish)	si:dkey-24n17.6	XP_005155589.1
Xenopus tropicalis (tropical clawed frog)	erap1	XP_002933475.2
Canis lupus familiaris (dog)	ERAP1	XP_005618143.1
Bos taurus (cattle)	ERAP1	NP_001095473.1
Gallus gallus (chicken)	ERAP1	XP_001232418.2
Homo sapiens (human)	ERAP1	NP_057526.3
Rattus norvegicus (Norway rat)	Erap1	NP_110463.1

Gene Ontology
Bibliography

Related articles in PubMed

Mice expressing human ERAP1 variants associated with ankylosing spondylitis have altered T-cell repertoires and NK cell functions, as well as increased in utero and perinatal mortality.
Rastall DPW, Alyaquob FS, O'Connell P, Pepelyayeva Y, Peters D, Godbehere-Roosa S, Pereira-Hicks C, Aldhamen YA, Amalfitano A
International immunology29(6)277-289(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we have shown that the loss of ERAAP leads to shifts in the nature and lengths of peptides presented by MHC I molecules on the cell surface
Title: ERAAP Shapes the Peptidome Associated with Classical and Nonclassical MHC Class I Molecules.

results suggest that several aminopeptidases play important roles in the maximum synthesis of NO in activated macrophages in a substrate peptide-dependent manner and ERAP1 is one of the aminopeptidases involved in the NO synthesis
Title: Substrate-dependent nitric oxide synthesis by secreted endoplasmic reticulum aminopeptidase 1 in macrophages.

Data show that endoplasmic reticulum protein 44 (ERp44) forms a mixed disulfide bond with aminopeptidase ERAP1 and controls the release of ERAP1 in a redox-dependent manner to control blood pressure.
Title: ERp44 Exerts Redox-Dependent Control of Blood Pressure at the ER.

This study clarifies ERAP1's role in shaping immunodominance through creation and destruction of peptides in vivo and demonstrates the functional significance of ERAP1 in modulating T-cell killing based upon this role.
Title: ERAP1 functions override the intrinsic selection of specific antigens as immunodominant peptides, thereby altering the potency of antigen-specific cytolytic and effector memory T-cell responses.

These results suggest that secretion of ERAP1 is mediated by toll-like receptors via induction of intermediate cytokines
Title: TLR-mediated secretion of endoplasmic reticulum aminopeptidase 1 from macrophages.

The following Erap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Erap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23016	NM_030711.5 Latest version!	Mus musculus endoplasmic reticulum aminopeptidase 1 (Erap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu23016	XM_006517476.4 Latest version!	Mus musculus endoplasmic reticulum aminopeptidase 1 (Erap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu86310	XM_030247471.1 Latest version!	Mus musculus endoplasmic reticulum aminopeptidase 1 (Erap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu23016	XM_006517476.3	Mus musculus endoplasmic reticulum aminopeptidase 1 (Erap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu23016	NM_030711.4	Mus musculus endoplasmic reticulum aminopeptidase 1 (Erap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Erap1 ( NM_030711.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_109636.1 Mus musculus endoplasmic reticulum aminopeptidase 1 (Erap1), mRNA.

Price & Availability↑

CloneID	OMu23016
Clone ID Related Accession (Same CDS sequence)	NM_030711.5 , XM_006517476.3 , XM_006517476.4 , NM_030711.4
Accession Version	NM_030711.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	endoplasmic reticulum aminopeptidase 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC138120.3. On Apr 14, 2018 this sequence version replaced NM_030711.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK036559.1, AK030329.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCTC TTCTTCCCCT AGTATTGACA TTTTTATCTG TGTCATCTCC TTCTTGGTGT 
CAGAACAGTG ATATAGAATC TCTAAAAGCT AGTAATGGAG ACTCATTCCC TTGGAATAAT 
ATGCGACTTC CTGAGTATAT GACCCCGATT CATTATGATC TCATGATCCA TGCAAACCTC 
AGCACTCTGA CTTTCTGGGG AAAAACAGAA GTAGAAATCA TAGCTAGCCG GCCCACCAGC 
ACCATTATTA TGCATAGTCA CCACCTGCAA ATATCTAAGG CCACCCTCAG AAGAGGAGCT 
GGAGAGATGC TGTCTGAAGA ACCACTGAAG GTCCTGGAAT ATCCTGCTCA TGAGCAAGTT 
GCACTGCTGG CTGCCCAGCC GCTTCTTGCT GGGTCCCTGT ACACAGTTAT CATCGACTAT 
GCTGCCAACC TGTCTGAGAG TTTCCATGGA TTCTATAAGA GCACCTACAG AACACAGGAA 
GGTGAAATGA GAATACTTGC AGCAACACAG TTTGAACCCA CAGCTGCTAG AATGGCTTTT 
CCCTGCTTTG ATGAACCTGC CCTCAAGGCA AGTTTTTCCA TCAAGATAAA GAGGGATCCA 
AGGCACCTGG CCATCTCCAA CATGCCCCTG GTGAAATCTG TGAATGTTGC TGAAGGACTC 
ATAGAAGACC ATTTTGACAT CACTGTGAAG ATGAGTACCT ACCTAGTGGC CTTCATCATT 
TCTGATTTTA AGTCTGTGAG CAAGATGACT AAGAGTGGAG TCAAGGTTTC TGTGTATGCT 
GTGCCAGACA AGATAAATCA AGCCGATTAT GCTCTGGATG CTGCAGTGAC TCTTCTAGAG 
TTTTATGAGG ATTATTTCAA CATTCCATAT CCTTTACCCA AGCAAGATCT TGCTGCAATC 
CCAGACTTTC AGTCTGGTGC TATGGAAAAC TGGGGACTGA CCACATACAG AGAGTCCTCT 
CTGTTATACG ATAAAGAAAA GTCTTCTGCA TCTAGTAAGC TTGGCATCAC AATGATTGTG 
TCCCATGAAC TGGCTCACCA GTGGTTCGGG AACCTGGTCA CCATGGAATG GTGGAATGAC 
CTTTGGCTCA ATGAAGGATT TGCCAAATTT ATGGAGTTTG TGTCTGTCAC TGTGACCCAT 
CCTGAACTAA AAGTTGAAGA CTATTTCTTT GGCAAGTGTT TTAATGCAAT GGAAGTTGAT 
GCATTAAACT CCTCTCACCC TGTATCCACA CCTGTGGAGA ATCCTGCGCA GATTCGGGAG 
ATGTTTGATG ATGTTTCTTA TGAAAAGGGA GCTTGTATTC TGAATATGCT AAGAGATTAT 
CTGAGTGCAG ATACATTTAA AAGAGGAATT GTACAGTATC TCCAAAAGTA TAGTTATAAA 
AACACAAAAA ACGAGGACCT GTGGAATAGC ATGATGCATA TTTGCCCTAC AGATGGCACA 
CAAACAATGG ATGGCTTCTG CTCTAGAAGC CAACACTCAT CATCAACCTC ACACTGGCGT 
CAGGAGGTTG TAGATGTAAA GACCATGATG AACACATGGA CACTGCAGAA GGGCTTTCCT 
CTGATAACCA TCACTGTGAG TGGGCGGAAT GTGCACATGA AGCAAGAACA CTACATGAAG 
GGTTCAGAAC GCTTCCCAGA GACTGGGTAT TTGTGGCATG TTCCACTGAC ATTCATTACC 
AGCAAATCAG ACTCAGTCCA GAGATTTTTG CTGAAGACAA AGACAGATGT GCTCATCCTC 
CCAGAAGCAG TGCAGTGGAT CAAATTTAAC GTGGGAATGA ATGGCTATTA CATTGTGCAT 
TACGCGGATG ATGGATGGGC TTCTCTGAGT GGCCTTTTAA AAGAAGCGCA CACAACAATC 
AGCAGTAATG ATCGGGCAAG TCTCATCAAC AATGCATTTC AGCTGGTCAG CATTGAAAAG 
TTATCAATAG AAAAAGCCCT GGATTTAACC CTGTACTTGA AAAATGAAAC TGAAATTATG 
CCCATATTCC AAGCTTTGAA TGAACTCATA CCTATGTATA AGTTAATGGA GAAAAGAGAT 
ATGATTGAGG TGGAAACTCA ATTTAAGGAC TTCCTTCTCA AGTTGCTGAA GGACCTTATT 
GATAAGCAGA CTTGGACAGA TGAAGGCTCT GTCTCAGAGA GGATGCTCAG GAGCCAGCTC 
CTCCTCCTCG CATGTGTGCG CAATTATCAG CCCTGTGTGC AGAGGGCAGA GCGCTATTTC 
AGAGAGTGGA AGTCATCTAA TGGAAACATG AGCATCCCTA TTGATGTGAC CTTGGCTGTG 
TTTGCTGTCG GGGCCCAAAA CACAGAAGGC TGGGATTTTC TTTATAGTAA ATATCAGTCT 
TCTTTGTCTA GTACTGAGAA AAGCCAAATT GAATTTTCCC TCTGTACAAG CAAAGATCCA 
GAAAAACTTC AGTGGCTTCT AGATCAAAGT TTTAAGGGAG AGATAATAAA AACTCAGGAA 
TTTCCACATA TTCTCACACT CATTGGCAGA AACCCAGTGG GTTATCCATT GGCCTGGAAG 
TTTCTGAGGG AAAATTGGAA TAAGCTTGTA CAGAAATTTG AACTTGGCTC ATCGTCCATA 
GCTCACATGG TAATGGGAAC AACAGACCAG TTTTCCACCA GAGCACGTCT TGAAGAGGTA 
AAAGGATTCT TCAGTTCCTT GAAAGAAAAC GGTTCTCAGC TCCGTTGTGT TCAACAAACC 
ATTGAGACCA TTGAAGAAAA CATACGATGG ATGGATAAGA ATTTTGATAA AATAAGACTG 
TGGCTGCAAA AAGAAAAGCC AGAGTTGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_109636.1
CDS	95..2887
Translation	MPSLLPLVLTFLSVSSPSWCQNSDIESLKASNGDSFPWNNMRLPEYMTPIHYDLMIHANLSTLTFWGKTEVEIIASRPTSTIIMHSHHLQISKATLRRGAGEMLSEEPLKVLEYPAHEQVALLAAQPLLAGSLYTVIIDYAANLSESFHGFYKSTYRTQEGEMRILAATQFEPTAARMAFPCFDEPALKASFSIKIKRDPRHLAISNMPLVKSVNVAEGLIEDHFDITVKMSTYLVAFIISDFKSVSKMTKSGVKVSVYAVPDKINQADYALDAAVTLLEFYEDYFNIPYPLPKQDLAAIPDFQSGAMENWGLTTYRESSLLYDKEKSSASSKLGITMIVSHELAHQWFGNLVTMEWWNDLWLNEGFAKFMEFVSVTVTHPELKVEDYFFGKCFNAMEVDALNSSHPVSTPVENPAQIREMFDDVSYEKGACILNMLRDYLSADTFKRGIVQYLQKYSYKNTKNEDLWNSMMHICPTDGTQTMDGFCSRSQHSSSTSHWRQEVVDVKTMMNTWTLQKGFPLITITVSGRNVHMKQEHYMKGSERFPETGYLWHVPLTFITSKSDSVQRFLLKTKTDVLILPEAVQWIKFNVGMNGYYIVHYADDGWASLSGLLKEAHTTISSNDRASLINNAFQLVSIEKLSIEKALDLTLYLKNETEIMPIFQALNELIPMYKLMEKRDMIEVETQFKDFLLKLLKDLIDKQTWTDEGSVSERMLRSQLLLLACVRNYQPCVQRAERYFREWKSSNGNMSIPIDVTLAVFAVGAQNTEGWDFLYSKYQSSLSSTEKSQIEFSLCTSKDPEKLQWLLDQSFKGEIIKTQEFPHILTLIGRNPVGYPLAWKFLRENWNKLVQKFELGSSSIAHMVMGTTDQFSTRARLEEVKGFFSSLKENGSQLRCVQQTIETIEENIRWMDKNFDKIRLWLQKEKPELL

Target ORF information:

RefSeq Version	NM_030711.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus endoplasmic reticulum aminopeptidase 1 (Erap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030711.5

ORF Insert Sequence:

ATGCCCTCTC TTCTTCCCCT AGTATTGACA TTTTTATCTG TGTCATCTCC TTCTTGGTGT 
CAGAACAGTG ATATAGAATC TCTAAAAGCT AGTAATGGAG ACTCATTCCC TTGGAATAAT 
ATGCGACTTC CTGAGTATAT GACCCCGATT CATTATGATC TCATGATCCA TGCAAACCTC 
AGCACTCTGA CTTTCTGGGG AAAAACAGAA GTAGAAATCA TAGCTAGCCG GCCCACCAGC 
ACCATTATTA TGCATAGTCA CCACCTGCAA ATATCTAAGG CCACCCTCAG AAGAGGAGCT 
GGAGAGATGC TGTCTGAAGA ACCACTGAAG GTCCTGGAAT ATCCTGCTCA TGAGCAAGTT 
GCACTGCTGG CTGCCCAGCC GCTTCTTGCT GGGTCCCTGT ACACAGTTAT CATCGACTAT 
GCTGCCAACC TGTCTGAGAG TTTCCATGGA TTCTATAAGA GCACCTACAG AACACAGGAA 
GGTGAAATGA GAATACTTGC AGCAACACAG TTTGAACCCA CAGCTGCTAG AATGGCTTTT 
CCCTGCTTTG ATGAACCTGC CCTCAAGGCA AGTTTTTCCA TCAAGATAAA GAGGGATCCA 
AGGCACCTGG CCATCTCCAA CATGCCCCTG GTGAAATCTG TGAATGTTGC TGAAGGACTC 
ATAGAAGACC ATTTTGACAT CACTGTGAAG ATGAGTACCT ACCTAGTGGC CTTCATCATT 
TCTGATTTTA AGTCTGTGAG CAAGATGACT AAGAGTGGAG TCAAGGTTTC TGTGTATGCT 
GTGCCAGACA AGATAAATCA AGCCGATTAT GCTCTGGATG CTGCAGTGAC TCTTCTAGAG 
TTTTATGAGG ATTATTTCAA CATTCCATAT CCTTTACCCA AGCAAGATCT TGCTGCAATC 
CCAGACTTTC AGTCTGGTGC TATGGAAAAC TGGGGACTGA CCACATACAG AGAGTCCTCT 
CTGTTATACG ATAAAGAAAA GTCTTCTGCA TCTAGTAAGC TTGGCATCAC AATGATTGTG 
TCCCATGAAC TGGCTCACCA GTGGTTCGGG AACCTGGTCA CCATGGAATG GTGGAATGAC 
CTTTGGCTCA ATGAAGGATT TGCCAAATTT ATGGAGTTTG TGTCTGTCAC TGTGACCCAT 
CCTGAACTAA AAGTTGAAGA CTATTTCTTT GGCAAGTGTT TTAATGCAAT GGAAGTTGAT 
GCATTAAACT CCTCTCACCC TGTATCCACA CCTGTGGAGA ATCCTGCGCA GATTCGGGAG 
ATGTTTGATG ATGTTTCTTA TGAAAAGGGA GCTTGTATTC TGAATATGCT AAGAGATTAT 
CTGAGTGCAG ATACATTTAA AAGAGGAATT GTACAGTATC TCCAAAAGTA TAGTTATAAA 
AACACAAAAA ACGAGGACCT GTGGAATAGC ATGATGCATA TTTGCCCTAC AGATGGCACA 
CAAACAATGG ATGGCTTCTG CTCTAGAAGC CAACACTCAT CATCAACCTC ACACTGGCGT 
CAGGAGGTTG TAGATGTAAA GACCATGATG AACACATGGA CACTGCAGAA GGGCTTTCCT 
CTGATAACCA TCACTGTGAG TGGGCGGAAT GTGCACATGA AGCAAGAACA CTACATGAAG 
GGTTCAGAAC GCTTCCCAGA GACTGGGTAT TTGTGGCATG TTCCACTGAC ATTCATTACC 
AGCAAATCAG ACTCAGTCCA GAGATTTTTG CTGAAGACAA AGACAGATGT GCTCATCCTC 
CCAGAAGCAG TGCAGTGGAT CAAATTTAAC GTGGGAATGA ATGGCTATTA CATTGTGCAT 
TACGCGGATG ATGGATGGGC TTCTCTGAGT GGCCTTTTAA AAGAAGCGCA CACAACAATC 
AGCAGTAATG ATCGGGCAAG TCTCATCAAC AATGCATTTC AGCTGGTCAG CATTGAAAAG 
TTATCAATAG AAAAAGCCCT GGATTTAACC CTGTACTTGA AAAATGAAAC TGAAATTATG 
CCCATATTCC AAGCTTTGAA TGAACTCATA CCTATGTATA AGTTAATGGA GAAAAGAGAT 
ATGATTGAGG TGGAAACTCA ATTTAAGGAC TTCCTTCTCA AGTTGCTGAA GGACCTTATT 
GATAAGCAGA CTTGGACAGA TGAAGGCTCT GTCTCAGAGA GGATGCTCAG GAGCCAGCTC 
CTCCTCCTCG CATGTGTGCG CAATTATCAG CCCTGTGTGC AGAGGGCAGA GCGCTATTTC 
AGAGAGTGGA AGTCATCTAA TGGAAACATG AGCATCCCTA TTGATGTGAC CTTGGCTGTG 
TTTGCTGTCG GGGCCCAAAA CACAGAAGGC TGGGATTTTC TTTATAGTAA ATATCAGTCT 
TCTTTGTCTA GTACTGAGAA AAGCCAAATT GAATTTTCCC TCTGTACAAG CAAAGATCCA 
GAAAAACTTC AGTGGCTTCT AGATCAAAGT TTTAAGGGAG AGATAATAAA AACTCAGGAA 
TTTCCACATA TTCTCACACT CATTGGCAGA AACCCAGTGG GTTATCCATT GGCCTGGAAG 
TTTCTGAGGG AAAATTGGAA TAAGCTTGTA CAGAAATTTG AACTTGGCTC ATCGTCCATA 
GCTCACATGG TAATGGGAAC AACAGACCAG TTTTCCACCA GAGCACGTCT TGAAGAGGTA 
AAAGGATTCT TCAGTTCCTT GAAAGAAAAC GGTTCTCAGC TCCGTTGTGT TCAACAAACC 
ATTGAGACCA TTGAAGAAAA CATACGATGG ATGGATAAGA ATTTTGATAA AATAAGACTG 
TGGCTGCAAA AAGAAAAGCC AGAGTTGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Erap1 ( NM_030711.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_109636.1 Mus musculus endoplasmic reticulum aminopeptidase 1 (Erap1), mRNA.

Price & Availability↑

CloneID	OMu23016
Clone ID Related Accession (Same CDS sequence)	NM_030711.5 , XM_006517476.3 , XM_006517476.4 , NM_030711.4
Accession Version	NM_030711.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Mus musculus(house mouse)
Product	endoplasmic reticulum aminopeptidase 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC138120.3. On May 1, 2007 this sequence version replaced NM_030711.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK030329.1, AK036559.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGCCCTCTC TTCTTCCCCT AGTATTGACA TTTTTATCTG TGTCATCTCC TTCTTGGTGT 
CAGAACAGTG ATATAGAATC TCTAAAAGCT AGTAATGGAG ACTCATTCCC TTGGAATAAT 
ATGCGACTTC CTGAGTATAT GACCCCGATT CATTATGATC TCATGATCCA TGCAAACCTC 
AGCACTCTGA CTTTCTGGGG AAAAACAGAA GTAGAAATCA TAGCTAGCCG GCCCACCAGC 
ACCATTATTA TGCATAGTCA CCACCTGCAA ATATCTAAGG CCACCCTCAG AAGAGGAGCT 
GGAGAGATGC TGTCTGAAGA ACCACTGAAG GTCCTGGAAT ATCCTGCTCA TGAGCAAGTT 
GCACTGCTGG CTGCCCAGCC GCTTCTTGCT GGGTCCCTGT ACACAGTTAT CATCGACTAT 
GCTGCCAACC TGTCTGAGAG TTTCCATGGA TTCTATAAGA GCACCTACAG AACACAGGAA 
GGTGAAATGA GAATACTTGC AGCAACACAG TTTGAACCCA CAGCTGCTAG AATGGCTTTT 
CCCTGCTTTG ATGAACCTGC CCTCAAGGCA AGTTTTTCCA TCAAGATAAA GAGGGATCCA 
AGGCACCTGG CCATCTCCAA CATGCCCCTG GTGAAATCTG TGAATGTTGC TGAAGGACTC 
ATAGAAGACC ATTTTGACAT CACTGTGAAG ATGAGTACCT ACCTAGTGGC CTTCATCATT 
TCTGATTTTA AGTCTGTGAG CAAGATGACT AAGAGTGGAG TCAAGGTTTC TGTGTATGCT 
GTGCCAGACA AGATAAATCA AGCCGATTAT GCTCTGGATG CTGCAGTGAC TCTTCTAGAG 
TTTTATGAGG ATTATTTCAA CATTCCATAT CCTTTACCCA AGCAAGATCT TGCTGCAATC 
CCAGACTTTC AGTCTGGTGC TATGGAAAAC TGGGGACTGA CCACATACAG AGAGTCCTCT 
CTGTTATACG ATAAAGAAAA GTCTTCTGCA TCTAGTAAGC TTGGCATCAC AATGATTGTG 
TCCCATGAAC TGGCTCACCA GTGGTTCGGG AACCTGGTCA CCATGGAATG GTGGAATGAC 
CTTTGGCTCA ATGAAGGATT TGCCAAATTT ATGGAGTTTG TGTCTGTCAC TGTGACCCAT 
CCTGAACTAA AAGTTGAAGA CTATTTCTTT GGCAAGTGTT TTAATGCAAT GGAAGTTGAT 
GCATTAAACT CCTCTCACCC TGTATCCACA CCTGTGGAGA ATCCTGCGCA GATTCGGGAG 
ATGTTTGATG ATGTTTCTTA TGAAAAGGGA GCTTGTATTC TGAATATGCT AAGAGATTAT 
CTGAGTGCAG ATACATTTAA AAGAGGAATT GTACAGTATC TCCAAAAGTA TAGTTATAAA 
AACACAAAAA ACGAGGACCT GTGGAATAGC ATGATGCATA TTTGCCCTAC AGATGGCACA 
CAAACAATGG ATGGCTTCTG CTCTAGAAGC CAACACTCAT CATCAACCTC ACACTGGCGT 
CAGGAGGTTG TAGATGTAAA GACCATGATG AACACATGGA CACTGCAGAA GGGCTTTCCT 
CTGATAACCA TCACTGTGAG TGGGCGGAAT GTGCACATGA AGCAAGAACA CTACATGAAG 
GGTTCAGAAC GCTTCCCAGA GACTGGGTAT TTGTGGCATG TTCCACTGAC ATTCATTACC 
AGCAAATCAG ACTCAGTCCA GAGATTTTTG CTGAAGACAA AGACAGATGT GCTCATCCTC 
CCAGAAGCAG TGCAGTGGAT CAAATTTAAC GTGGGAATGA ATGGCTATTA CATTGTGCAT 
TACGCGGATG ATGGATGGGC TTCTCTGAGT GGCCTTTTAA AAGAAGCGCA CACAACAATC 
AGCAGTAATG ATCGGGCAAG TCTCATCAAC AATGCATTTC AGCTGGTCAG CATTGAAAAG 
TTATCAATAG AAAAAGCCCT GGATTTAACC CTGTACTTGA AAAATGAAAC TGAAATTATG 
CCCATATTCC AAGCTTTGAA TGAACTCATA CCTATGTATA AGTTAATGGA GAAAAGAGAT 
ATGATTGAGG TGGAAACTCA ATTTAAGGAC TTCCTTCTCA AGTTGCTGAA GGACCTTATT 
GATAAGCAGA CTTGGACAGA TGAAGGCTCT GTCTCAGAGA GGATGCTCAG GAGCCAGCTC 
CTCCTCCTCG CATGTGTGCG CAATTATCAG CCCTGTGTGC AGAGGGCAGA GCGCTATTTC 
AGAGAGTGGA AGTCATCTAA TGGAAACATG AGCATCCCTA TTGATGTGAC CTTGGCTGTG 
TTTGCTGTCG GGGCCCAAAA CACAGAAGGC TGGGATTTTC TTTATAGTAA ATATCAGTCT 
TCTTTGTCTA GTACTGAGAA AAGCCAAATT GAATTTTCCC TCTGTACAAG CAAAGATCCA 
GAAAAACTTC AGTGGCTTCT AGATCAAAGT TTTAAGGGAG AGATAATAAA AACTCAGGAA 
TTTCCACATA TTCTCACACT CATTGGCAGA AACCCAGTGG GTTATCCATT GGCCTGGAAG 
TTTCTGAGGG AAAATTGGAA TAAGCTTGTA CAGAAATTTG AACTTGGCTC ATCGTCCATA 
GCTCACATGG TAATGGGAAC AACAGACCAG TTTTCCACCA GAGCACGTCT TGAAGAGGTA 
AAAGGATTCT TCAGTTCCTT GAAAGAAAAC GGTTCTCAGC TCCGTTGTGT TCAACAAACC 
ATTGAGACCA TTGAAGAAAA CATACGATGG ATGGATAAGA ATTTTGATAA AATAAGACTG 
TGGCTGCAAA AAGAAAAGCC AGAGTTGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_109636.1
CDS	76..2868
Translation	MPSLLPLVLTFLSVSSPSWCQNSDIESLKASNGDSFPWNNMRLPEYMTPIHYDLMIHANLSTLTFWGKTEVEIIASRPTSTIIMHSHHLQISKATLRRGAGEMLSEEPLKVLEYPAHEQVALLAAQPLLAGSLYTVIIDYAANLSESFHGFYKSTYRTQEGEMRILAATQFEPTAARMAFPCFDEPALKASFSIKIKRDPRHLAISNMPLVKSVNVAEGLIEDHFDITVKMSTYLVAFIISDFKSVSKMTKSGVKVSVYAVPDKINQADYALDAAVTLLEFYEDYFNIPYPLPKQDLAAIPDFQSGAMENWGLTTYRESSLLYDKEKSSASSKLGITMIVSHELAHQWFGNLVTMEWWNDLWLNEGFAKFMEFVSVTVTHPELKVEDYFFGKCFNAMEVDALNSSHPVSTPVENPAQIREMFDDVSYEKGACILNMLRDYLSADTFKRGIVQYLQKYSYKNTKNEDLWNSMMHICPTDGTQTMDGFCSRSQHSSSTSHWRQEVVDVKTMMNTWTLQKGFPLITITVSGRNVHMKQEHYMKGSERFPETGYLWHVPLTFITSKSDSVQRFLLKTKTDVLILPEAVQWIKFNVGMNGYYIVHYADDGWASLSGLLKEAHTTISSNDRASLINNAFQLVSIEKLSIEKALDLTLYLKNETEIMPIFQALNELIPMYKLMEKRDMIEVETQFKDFLLKLLKDLIDKQTWTDEGSVSERMLRSQLLLLACVRNYQPCVQRAERYFREWKSSNGNMSIPIDVTLAVFAVGAQNTEGWDFLYSKYQSSLSSTEKSQIEFSLCTSKDPEKLQWLLDQSFKGEIIKTQEFPHILTLIGRNPVGYPLAWKFLRENWNKLVQKFELGSSSIAHMVMGTTDQFSTRARLEEVKGFFSSLKENGSQLRCVQQTIETIEENIRWMDKNFDKIRLWLQKEKPELL

Target ORF information:

RefSeq Version	NM_030711.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus endoplasmic reticulum aminopeptidase 1 (Erap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030711.4

ORF Insert Sequence:

ATGCCCTCTC TTCTTCCCCT AGTATTGACA TTTTTATCTG TGTCATCTCC TTCTTGGTGT 
CAGAACAGTG ATATAGAATC TCTAAAAGCT AGTAATGGAG ACTCATTCCC TTGGAATAAT 
ATGCGACTTC CTGAGTATAT GACCCCGATT CATTATGATC TCATGATCCA TGCAAACCTC 
AGCACTCTGA CTTTCTGGGG AAAAACAGAA GTAGAAATCA TAGCTAGCCG GCCCACCAGC 
ACCATTATTA TGCATAGTCA CCACCTGCAA ATATCTAAGG CCACCCTCAG AAGAGGAGCT 
GGAGAGATGC TGTCTGAAGA ACCACTGAAG GTCCTGGAAT ATCCTGCTCA TGAGCAAGTT 
GCACTGCTGG CTGCCCAGCC GCTTCTTGCT GGGTCCCTGT ACACAGTTAT CATCGACTAT 
GCTGCCAACC TGTCTGAGAG TTTCCATGGA TTCTATAAGA GCACCTACAG AACACAGGAA 
GGTGAAATGA GAATACTTGC AGCAACACAG TTTGAACCCA CAGCTGCTAG AATGGCTTTT 
CCCTGCTTTG ATGAACCTGC CCTCAAGGCA AGTTTTTCCA TCAAGATAAA GAGGGATCCA 
AGGCACCTGG CCATCTCCAA CATGCCCCTG GTGAAATCTG TGAATGTTGC TGAAGGACTC 
ATAGAAGACC ATTTTGACAT CACTGTGAAG ATGAGTACCT ACCTAGTGGC CTTCATCATT 
TCTGATTTTA AGTCTGTGAG CAAGATGACT AAGAGTGGAG TCAAGGTTTC TGTGTATGCT 
GTGCCAGACA AGATAAATCA AGCCGATTAT GCTCTGGATG CTGCAGTGAC TCTTCTAGAG 
TTTTATGAGG ATTATTTCAA CATTCCATAT CCTTTACCCA AGCAAGATCT TGCTGCAATC 
CCAGACTTTC AGTCTGGTGC TATGGAAAAC TGGGGACTGA CCACATACAG AGAGTCCTCT 
CTGTTATACG ATAAAGAAAA GTCTTCTGCA TCTAGTAAGC TTGGCATCAC AATGATTGTG 
TCCCATGAAC TGGCTCACCA GTGGTTCGGG AACCTGGTCA CCATGGAATG GTGGAATGAC 
CTTTGGCTCA ATGAAGGATT TGCCAAATTT ATGGAGTTTG TGTCTGTCAC TGTGACCCAT 
CCTGAACTAA AAGTTGAAGA CTATTTCTTT GGCAAGTGTT TTAATGCAAT GGAAGTTGAT 
GCATTAAACT CCTCTCACCC TGTATCCACA CCTGTGGAGA ATCCTGCGCA GATTCGGGAG 
ATGTTTGATG ATGTTTCTTA TGAAAAGGGA GCTTGTATTC TGAATATGCT AAGAGATTAT 
CTGAGTGCAG ATACATTTAA AAGAGGAATT GTACAGTATC TCCAAAAGTA TAGTTATAAA 
AACACAAAAA ACGAGGACCT GTGGAATAGC ATGATGCATA TTTGCCCTAC AGATGGCACA 
CAAACAATGG ATGGCTTCTG CTCTAGAAGC CAACACTCAT CATCAACCTC ACACTGGCGT 
CAGGAGGTTG TAGATGTAAA GACCATGATG AACACATGGA CACTGCAGAA GGGCTTTCCT 
CTGATAACCA TCACTGTGAG TGGGCGGAAT GTGCACATGA AGCAAGAACA CTACATGAAG 
GGTTCAGAAC GCTTCCCAGA GACTGGGTAT TTGTGGCATG TTCCACTGAC ATTCATTACC 
AGCAAATCAG ACTCAGTCCA GAGATTTTTG CTGAAGACAA AGACAGATGT GCTCATCCTC 
CCAGAAGCAG TGCAGTGGAT CAAATTTAAC GTGGGAATGA ATGGCTATTA CATTGTGCAT 
TACGCGGATG ATGGATGGGC TTCTCTGAGT GGCCTTTTAA AAGAAGCGCA CACAACAATC 
AGCAGTAATG ATCGGGCAAG TCTCATCAAC AATGCATTTC AGCTGGTCAG CATTGAAAAG 
TTATCAATAG AAAAAGCCCT GGATTTAACC CTGTACTTGA AAAATGAAAC TGAAATTATG 
CCCATATTCC AAGCTTTGAA TGAACTCATA CCTATGTATA AGTTAATGGA GAAAAGAGAT 
ATGATTGAGG TGGAAACTCA ATTTAAGGAC TTCCTTCTCA AGTTGCTGAA GGACCTTATT 
GATAAGCAGA CTTGGACAGA TGAAGGCTCT GTCTCAGAGA GGATGCTCAG GAGCCAGCTC 
CTCCTCCTCG CATGTGTGCG CAATTATCAG CCCTGTGTGC AGAGGGCAGA GCGCTATTTC 
AGAGAGTGGA AGTCATCTAA TGGAAACATG AGCATCCCTA TTGATGTGAC CTTGGCTGTG 
TTTGCTGTCG GGGCCCAAAA CACAGAAGGC TGGGATTTTC TTTATAGTAA ATATCAGTCT 
TCTTTGTCTA GTACTGAGAA AAGCCAAATT GAATTTTCCC TCTGTACAAG CAAAGATCCA 
GAAAAACTTC AGTGGCTTCT AGATCAAAGT TTTAAGGGAG AGATAATAAA AACTCAGGAA 
TTTCCACATA TTCTCACACT CATTGGCAGA AACCCAGTGG GTTATCCATT GGCCTGGAAG 
TTTCTGAGGG AAAATTGGAA TAAGCTTGTA CAGAAATTTG AACTTGGCTC ATCGTCCATA 
GCTCACATGG TAATGGGAAC AACAGACCAG TTTTCCACCA GAGCACGTCT TGAAGAGGTA 
AAAGGATTCT TCAGTTCCTT GAAAGAAAAC GGTTCTCAGC TCCGTTGTGT TCAACAAACC 
ATTGAGACCA TTGAAGAAAA CATACGATGG ATGGATAAGA ATTTTGATAA AATAAGACTG 
TGGCTGCAAA AAGAAAAGCC AGAGTTGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Erap1 ( XM_006517476.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517539.1 Mus musculus endoplasmic reticulum aminopeptidase 1 (Erap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu23016
Clone ID Related Accession (Same CDS sequence)	NM_030711.5 , XM_006517476.3 , XM_006517476.4 , NM_030711.4
Accession Version	XM_006517476.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	endoplasmic reticulum aminopeptidase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517476.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTC TTCTTCCCCT AGTATTGACA TTTTTATCTG TGTCATCTCC TTCTTGGTGT 
CAGAACAGTG ATATAGAATC TCTAAAAGCT AGTAATGGAG ACTCATTCCC TTGGAATAAT 
ATGCGACTTC CTGAGTATAT GACCCCGATT CATTATGATC TCATGATCCA TGCAAACCTC 
AGCACTCTGA CTTTCTGGGG AAAAACAGAA GTAGAAATCA TAGCTAGCCG GCCCACCAGC 
ACCATTATTA TGCATAGTCA CCACCTGCAA ATATCTAAGG CCACCCTCAG AAGAGGAGCT 
GGAGAGATGC TGTCTGAAGA ACCACTGAAG GTCCTGGAAT ATCCTGCTCA TGAGCAAGTT 
GCACTGCTGG CTGCCCAGCC GCTTCTTGCT GGGTCCCTGT ACACAGTTAT CATCGACTAT 
GCTGCCAACC TGTCTGAGAG TTTCCATGGA TTCTATAAGA GCACCTACAG AACACAGGAA 
GGTGAAATGA GAATACTTGC AGCAACACAG TTTGAACCCA CAGCTGCTAG AATGGCTTTT 
CCCTGCTTTG ATGAACCTGC CCTCAAGGCA AGTTTTTCCA TCAAGATAAA GAGGGATCCA 
AGGCACCTGG CCATCTCCAA CATGCCCCTG GTGAAATCTG TGAATGTTGC TGAAGGACTC 
ATAGAAGACC ATTTTGACAT CACTGTGAAG ATGAGTACCT ACCTAGTGGC CTTCATCATT 
TCTGATTTTA AGTCTGTGAG CAAGATGACT AAGAGTGGAG TCAAGGTTTC TGTGTATGCT 
GTGCCAGACA AGATAAATCA AGCCGATTAT GCTCTGGATG CTGCAGTGAC TCTTCTAGAG 
TTTTATGAGG ATTATTTCAA CATTCCATAT CCTTTACCCA AGCAAGATCT TGCTGCAATC 
CCAGACTTTC AGTCTGGTGC TATGGAAAAC TGGGGACTGA CCACATACAG AGAGTCCTCT 
CTGTTATACG ATAAAGAAAA GTCTTCTGCA TCTAGTAAGC TTGGCATCAC AATGATTGTG 
TCCCATGAAC TGGCTCACCA GTGGTTCGGG AACCTGGTCA CCATGGAATG GTGGAATGAC 
CTTTGGCTCA ATGAAGGATT TGCCAAATTT ATGGAGTTTG TGTCTGTCAC TGTGACCCAT 
CCTGAACTAA AAGTTGAAGA CTATTTCTTT GGCAAGTGTT TTAATGCAAT GGAAGTTGAT 
GCATTAAACT CCTCTCACCC TGTATCCACA CCTGTGGAGA ATCCTGCGCA GATTCGGGAG 
ATGTTTGATG ATGTTTCTTA TGAAAAGGGA GCTTGTATTC TGAATATGCT AAGAGATTAT 
CTGAGTGCAG ATACATTTAA AAGAGGAATT GTACAGTATC TCCAAAAGTA TAGTTATAAA 
AACACAAAAA ACGAGGACCT GTGGAATAGC ATGATGCATA TTTGCCCTAC AGATGGCACA 
CAAACAATGG ATGGCTTCTG CTCTAGAAGC CAACACTCAT CATCAACCTC ACACTGGCGT 
CAGGAGGTTG TAGATGTAAA GACCATGATG AACACATGGA CACTGCAGAA GGGCTTTCCT 
CTGATAACCA TCACTGTGAG TGGGCGGAAT GTGCACATGA AGCAAGAACA CTACATGAAG 
GGTTCAGAAC GCTTCCCAGA GACTGGGTAT TTGTGGCATG TTCCACTGAC ATTCATTACC 
AGCAAATCAG ACTCAGTCCA GAGATTTTTG CTGAAGACAA AGACAGATGT GCTCATCCTC 
CCAGAAGCAG TGCAGTGGAT CAAATTTAAC GTGGGAATGA ATGGCTATTA CATTGTGCAT 
TACGCGGATG ATGGATGGGC TTCTCTGAGT GGCCTTTTAA AAGAAGCGCA CACAACAATC 
AGCAGTAATG ATCGGGCAAG TCTCATCAAC AATGCATTTC AGCTGGTCAG CATTGAAAAG 
TTATCAATAG AAAAAGCCCT GGATTTAACC CTGTACTTGA AAAATGAAAC TGAAATTATG 
CCCATATTCC AAGCTTTGAA TGAACTCATA CCTATGTATA AGTTAATGGA GAAAAGAGAT 
ATGATTGAGG TGGAAACTCA ATTTAAGGAC TTCCTTCTCA AGTTGCTGAA GGACCTTATT 
GATAAGCAGA CTTGGACAGA TGAAGGCTCT GTCTCAGAGA GGATGCTCAG GAGCCAGCTC 
CTCCTCCTCG CATGTGTGCG CAATTATCAG CCCTGTGTGC AGAGGGCAGA GCGCTATTTC 
AGAGAGTGGA AGTCATCTAA TGGAAACATG AGCATCCCTA TTGATGTGAC CTTGGCTGTG 
TTTGCTGTCG GGGCCCAAAA CACAGAAGGC TGGGATTTTC TTTATAGTAA ATATCAGTCT 
TCTTTGTCTA GTACTGAGAA AAGCCAAATT GAATTTTCCC TCTGTACAAG CAAAGATCCA 
GAAAAACTTC AGTGGCTTCT AGATCAAAGT TTTAAGGGAG AGATAATAAA AACTCAGGAA 
TTTCCACATA TTCTCACACT CATTGGCAGA AACCCAGTGG GTTATCCATT GGCCTGGAAG 
TTTCTGAGGG AAAATTGGAA TAAGCTTGTA CAGAAATTTG AACTTGGCTC ATCGTCCATA 
GCTCACATGG TAATGGGAAC AACAGACCAG TTTTCCACCA GAGCACGTCT TGAAGAGGTA 
AAAGGATTCT TCAGTTCCTT GAAAGAAAAC GGTTCTCAGC TCCGTTGTGT TCAACAAACC 
ATTGAGACCA TTGAAGAAAA CATACGATGG ATGGATAAGA ATTTTGATAA AATAAGACTG 
TGGCTGCAAA AAGAAAAGCC AGAGTTGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517539.1
CDS	366..3158
Translation	MPSLLPLVLTFLSVSSPSWCQNSDIESLKASNGDSFPWNNMRLPEYMTPIHYDLMIHANLSTLTFWGKTEVEIIASRPTSTIIMHSHHLQISKATLRRGAGEMLSEEPLKVLEYPAHEQVALLAAQPLLAGSLYTVIIDYAANLSESFHGFYKSTYRTQEGEMRILAATQFEPTAARMAFPCFDEPALKASFSIKIKRDPRHLAISNMPLVKSVNVAEGLIEDHFDITVKMSTYLVAFIISDFKSVSKMTKSGVKVSVYAVPDKINQADYALDAAVTLLEFYEDYFNIPYPLPKQDLAAIPDFQSGAMENWGLTTYRESSLLYDKEKSSASSKLGITMIVSHELAHQWFGNLVTMEWWNDLWLNEGFAKFMEFVSVTVTHPELKVEDYFFGKCFNAMEVDALNSSHPVSTPVENPAQIREMFDDVSYEKGACILNMLRDYLSADTFKRGIVQYLQKYSYKNTKNEDLWNSMMHICPTDGTQTMDGFCSRSQHSSSTSHWRQEVVDVKTMMNTWTLQKGFPLITITVSGRNVHMKQEHYMKGSERFPETGYLWHVPLTFITSKSDSVQRFLLKTKTDVLILPEAVQWIKFNVGMNGYYIVHYADDGWASLSGLLKEAHTTISSNDRASLINNAFQLVSIEKLSIEKALDLTLYLKNETEIMPIFQALNELIPMYKLMEKRDMIEVETQFKDFLLKLLKDLIDKQTWTDEGSVSERMLRSQLLLLACVRNYQPCVQRAERYFREWKSSNGNMSIPIDVTLAVFAVGAQNTEGWDFLYSKYQSSLSSTEKSQIEFSLCTSKDPEKLQWLLDQSFKGEIIKTQEFPHILTLIGRNPVGYPLAWKFLRENWNKLVQKFELGSSSIAHMVMGTTDQFSTRARLEEVKGFFSSLKENGSQLRCVQQTIETIEENIRWMDKNFDKIRLWLQKEKPELL

Target ORF information:

RefSeq Version	XM_006517476.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus endoplasmic reticulum aminopeptidase 1 (Erap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517476.3

ORF Insert Sequence:

ATGCCCTCTC TTCTTCCCCT AGTATTGACA TTTTTATCTG TGTCATCTCC TTCTTGGTGT 
CAGAACAGTG ATATAGAATC TCTAAAAGCT AGTAATGGAG ACTCATTCCC TTGGAATAAT 
ATGCGACTTC CTGAGTATAT GACCCCGATT CATTATGATC TCATGATCCA TGCAAACCTC 
AGCACTCTGA CTTTCTGGGG AAAAACAGAA GTAGAAATCA TAGCTAGCCG GCCCACCAGC 
ACCATTATTA TGCATAGTCA CCACCTGCAA ATATCTAAGG CCACCCTCAG AAGAGGAGCT 
GGAGAGATGC TGTCTGAAGA ACCACTGAAG GTCCTGGAAT ATCCTGCTCA TGAGCAAGTT 
GCACTGCTGG CTGCCCAGCC GCTTCTTGCT GGGTCCCTGT ACACAGTTAT CATCGACTAT 
GCTGCCAACC TGTCTGAGAG TTTCCATGGA TTCTATAAGA GCACCTACAG AACACAGGAA 
GGTGAAATGA GAATACTTGC AGCAACACAG TTTGAACCCA CAGCTGCTAG AATGGCTTTT 
CCCTGCTTTG ATGAACCTGC CCTCAAGGCA AGTTTTTCCA TCAAGATAAA GAGGGATCCA 
AGGCACCTGG CCATCTCCAA CATGCCCCTG GTGAAATCTG TGAATGTTGC TGAAGGACTC 
ATAGAAGACC ATTTTGACAT CACTGTGAAG ATGAGTACCT ACCTAGTGGC CTTCATCATT 
TCTGATTTTA AGTCTGTGAG CAAGATGACT AAGAGTGGAG TCAAGGTTTC TGTGTATGCT 
GTGCCAGACA AGATAAATCA AGCCGATTAT GCTCTGGATG CTGCAGTGAC TCTTCTAGAG 
TTTTATGAGG ATTATTTCAA CATTCCATAT CCTTTACCCA AGCAAGATCT TGCTGCAATC 
CCAGACTTTC AGTCTGGTGC TATGGAAAAC TGGGGACTGA CCACATACAG AGAGTCCTCT 
CTGTTATACG ATAAAGAAAA GTCTTCTGCA TCTAGTAAGC TTGGCATCAC AATGATTGTG 
TCCCATGAAC TGGCTCACCA GTGGTTCGGG AACCTGGTCA CCATGGAATG GTGGAATGAC 
CTTTGGCTCA ATGAAGGATT TGCCAAATTT ATGGAGTTTG TGTCTGTCAC TGTGACCCAT 
CCTGAACTAA AAGTTGAAGA CTATTTCTTT GGCAAGTGTT TTAATGCAAT GGAAGTTGAT 
GCATTAAACT CCTCTCACCC TGTATCCACA CCTGTGGAGA ATCCTGCGCA GATTCGGGAG 
ATGTTTGATG ATGTTTCTTA TGAAAAGGGA GCTTGTATTC TGAATATGCT AAGAGATTAT 
CTGAGTGCAG ATACATTTAA AAGAGGAATT GTACAGTATC TCCAAAAGTA TAGTTATAAA 
AACACAAAAA ACGAGGACCT GTGGAATAGC ATGATGCATA TTTGCCCTAC AGATGGCACA 
CAAACAATGG ATGGCTTCTG CTCTAGAAGC CAACACTCAT CATCAACCTC ACACTGGCGT 
CAGGAGGTTG TAGATGTAAA GACCATGATG AACACATGGA CACTGCAGAA GGGCTTTCCT 
CTGATAACCA TCACTGTGAG TGGGCGGAAT GTGCACATGA AGCAAGAACA CTACATGAAG 
GGTTCAGAAC GCTTCCCAGA GACTGGGTAT TTGTGGCATG TTCCACTGAC ATTCATTACC 
AGCAAATCAG ACTCAGTCCA GAGATTTTTG CTGAAGACAA AGACAGATGT GCTCATCCTC 
CCAGAAGCAG TGCAGTGGAT CAAATTTAAC GTGGGAATGA ATGGCTATTA CATTGTGCAT 
TACGCGGATG ATGGATGGGC TTCTCTGAGT GGCCTTTTAA AAGAAGCGCA CACAACAATC 
AGCAGTAATG ATCGGGCAAG TCTCATCAAC AATGCATTTC AGCTGGTCAG CATTGAAAAG 
TTATCAATAG AAAAAGCCCT GGATTTAACC CTGTACTTGA AAAATGAAAC TGAAATTATG 
CCCATATTCC AAGCTTTGAA TGAACTCATA CCTATGTATA AGTTAATGGA GAAAAGAGAT 
ATGATTGAGG TGGAAACTCA ATTTAAGGAC TTCCTTCTCA AGTTGCTGAA GGACCTTATT 
GATAAGCAGA CTTGGACAGA TGAAGGCTCT GTCTCAGAGA GGATGCTCAG GAGCCAGCTC 
CTCCTCCTCG CATGTGTGCG CAATTATCAG CCCTGTGTGC AGAGGGCAGA GCGCTATTTC 
AGAGAGTGGA AGTCATCTAA TGGAAACATG AGCATCCCTA TTGATGTGAC CTTGGCTGTG 
TTTGCTGTCG GGGCCCAAAA CACAGAAGGC TGGGATTTTC TTTATAGTAA ATATCAGTCT 
TCTTTGTCTA GTACTGAGAA AAGCCAAATT GAATTTTCCC TCTGTACAAG CAAAGATCCA 
GAAAAACTTC AGTGGCTTCT AGATCAAAGT TTTAAGGGAG AGATAATAAA AACTCAGGAA 
TTTCCACATA TTCTCACACT CATTGGCAGA AACCCAGTGG GTTATCCATT GGCCTGGAAG 
TTTCTGAGGG AAAATTGGAA TAAGCTTGTA CAGAAATTTG AACTTGGCTC ATCGTCCATA 
GCTCACATGG TAATGGGAAC AACAGACCAG TTTTCCACCA GAGCACGTCT TGAAGAGGTA 
AAAGGATTCT TCAGTTCCTT GAAAGAAAAC GGTTCTCAGC TCCGTTGTGT TCAACAAACC 
ATTGAGACCA TTGAAGAAAA CATACGATGG ATGGATAAGA ATTTTGATAA AATAAGACTG 
TGGCTGCAAA AAGAAAAGCC AGAGTTGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Erap1 ( XM_006517476.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517539.1 Mus musculus endoplasmic reticulum aminopeptidase 1 (Erap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu23016
Clone ID Related Accession (Same CDS sequence)	NM_030711.5 , XM_006517476.3 , XM_006517476.4 , NM_030711.4
Accession Version	XM_006517476.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	endoplasmic reticulum aminopeptidase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517476.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTC TTCTTCCCCT AGTATTGACA TTTTTATCTG TGTCATCTCC TTCTTGGTGT 
CAGAACAGTG ATATAGAATC TCTAAAAGCT AGTAATGGAG ACTCATTCCC TTGGAATAAT 
ATGCGACTTC CTGAGTATAT GACCCCGATT CATTATGATC TCATGATCCA TGCAAACCTC 
AGCACTCTGA CTTTCTGGGG AAAAACAGAA GTAGAAATCA TAGCTAGCCG GCCCACCAGC 
ACCATTATTA TGCATAGTCA CCACCTGCAA ATATCTAAGG CCACCCTCAG AAGAGGAGCT 
GGAGAGATGC TGTCTGAAGA ACCACTGAAG GTCCTGGAAT ATCCTGCTCA TGAGCAAGTT 
GCACTGCTGG CTGCCCAGCC GCTTCTTGCT GGGTCCCTGT ACACAGTTAT CATCGACTAT 
GCTGCCAACC TGTCTGAGAG TTTCCATGGA TTCTATAAGA GCACCTACAG AACACAGGAA 
GGTGAAATGA GAATACTTGC AGCAACACAG TTTGAACCCA CAGCTGCTAG AATGGCTTTT 
CCCTGCTTTG ATGAACCTGC CCTCAAGGCA AGTTTTTCCA TCAAGATAAA GAGGGATCCA 
AGGCACCTGG CCATCTCCAA CATGCCCCTG GTGAAATCTG TGAATGTTGC TGAAGGACTC 
ATAGAAGACC ATTTTGACAT CACTGTGAAG ATGAGTACCT ACCTAGTGGC CTTCATCATT 
TCTGATTTTA AGTCTGTGAG CAAGATGACT AAGAGTGGAG TCAAGGTTTC TGTGTATGCT 
GTGCCAGACA AGATAAATCA AGCCGATTAT GCTCTGGATG CTGCAGTGAC TCTTCTAGAG 
TTTTATGAGG ATTATTTCAA CATTCCATAT CCTTTACCCA AGCAAGATCT TGCTGCAATC 
CCAGACTTTC AGTCTGGTGC TATGGAAAAC TGGGGACTGA CCACATACAG AGAGTCCTCT 
CTGTTATACG ATAAAGAAAA GTCTTCTGCA TCTAGTAAGC TTGGCATCAC AATGATTGTG 
TCCCATGAAC TGGCTCACCA GTGGTTCGGG AACCTGGTCA CCATGGAATG GTGGAATGAC 
CTTTGGCTCA ATGAAGGATT TGCCAAATTT ATGGAGTTTG TGTCTGTCAC TGTGACCCAT 
CCTGAACTAA AAGTTGAAGA CTATTTCTTT GGCAAGTGTT TTAATGCAAT GGAAGTTGAT 
GCATTAAACT CCTCTCACCC TGTATCCACA CCTGTGGAGA ATCCTGCGCA GATTCGGGAG 
ATGTTTGATG ATGTTTCTTA TGAAAAGGGA GCTTGTATTC TGAATATGCT AAGAGATTAT 
CTGAGTGCAG ATACATTTAA AAGAGGAATT GTACAGTATC TCCAAAAGTA TAGTTATAAA 
AACACAAAAA ACGAGGACCT GTGGAATAGC ATGATGCATA TTTGCCCTAC AGATGGCACA 
CAAACAATGG ATGGCTTCTG CTCTAGAAGC CAACACTCAT CATCAACCTC ACACTGGCGT 
CAGGAGGTTG TAGATGTAAA GACCATGATG AACACATGGA CACTGCAGAA GGGCTTTCCT 
CTGATAACCA TCACTGTGAG TGGGCGGAAT GTGCACATGA AGCAAGAACA CTACATGAAG 
GGTTCAGAAC GCTTCCCAGA GACTGGGTAT TTGTGGCATG TTCCACTGAC ATTCATTACC 
AGCAAATCAG ACTCAGTCCA GAGATTTTTG CTGAAGACAA AGACAGATGT GCTCATCCTC 
CCAGAAGCAG TGCAGTGGAT CAAATTTAAC GTGGGAATGA ATGGCTATTA CATTGTGCAT 
TACGCGGATG ATGGATGGGC TTCTCTGAGT GGCCTTTTAA AAGAAGCGCA CACAACAATC 
AGCAGTAATG ATCGGGCAAG TCTCATCAAC AATGCATTTC AGCTGGTCAG CATTGAAAAG 
TTATCAATAG AAAAAGCCCT GGATTTAACC CTGTACTTGA AAAATGAAAC TGAAATTATG 
CCCATATTCC AAGCTTTGAA TGAACTCATA CCTATGTATA AGTTAATGGA GAAAAGAGAT 
ATGATTGAGG TGGAAACTCA ATTTAAGGAC TTCCTTCTCA AGTTGCTGAA GGACCTTATT 
GATAAGCAGA CTTGGACAGA TGAAGGCTCT GTCTCAGAGA GGATGCTCAG GAGCCAGCTC 
CTCCTCCTCG CATGTGTGCG CAATTATCAG CCCTGTGTGC AGAGGGCAGA GCGCTATTTC 
AGAGAGTGGA AGTCATCTAA TGGAAACATG AGCATCCCTA TTGATGTGAC CTTGGCTGTG 
TTTGCTGTCG GGGCCCAAAA CACAGAAGGC TGGGATTTTC TTTATAGTAA ATATCAGTCT 
TCTTTGTCTA GTACTGAGAA AAGCCAAATT GAATTTTCCC TCTGTACAAG CAAAGATCCA 
GAAAAACTTC AGTGGCTTCT AGATCAAAGT TTTAAGGGAG AGATAATAAA AACTCAGGAA 
TTTCCACATA TTCTCACACT CATTGGCAGA AACCCAGTGG GTTATCCATT GGCCTGGAAG 
TTTCTGAGGG AAAATTGGAA TAAGCTTGTA CAGAAATTTG AACTTGGCTC ATCGTCCATA 
GCTCACATGG TAATGGGAAC AACAGACCAG TTTTCCACCA GAGCACGTCT TGAAGAGGTA 
AAAGGATTCT TCAGTTCCTT GAAAGAAAAC GGTTCTCAGC TCCGTTGTGT TCAACAAACC 
ATTGAGACCA TTGAAGAAAA CATACGATGG ATGGATAAGA ATTTTGATAA AATAAGACTG 
TGGCTGCAAA AAGAAAAGCC AGAGTTGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517539.1
CDS	348..3140
Translation	MPSLLPLVLTFLSVSSPSWCQNSDIESLKASNGDSFPWNNMRLPEYMTPIHYDLMIHANLSTLTFWGKTEVEIIASRPTSTIIMHSHHLQISKATLRRGAGEMLSEEPLKVLEYPAHEQVALLAAQPLLAGSLYTVIIDYAANLSESFHGFYKSTYRTQEGEMRILAATQFEPTAARMAFPCFDEPALKASFSIKIKRDPRHLAISNMPLVKSVNVAEGLIEDHFDITVKMSTYLVAFIISDFKSVSKMTKSGVKVSVYAVPDKINQADYALDAAVTLLEFYEDYFNIPYPLPKQDLAAIPDFQSGAMENWGLTTYRESSLLYDKEKSSASSKLGITMIVSHELAHQWFGNLVTMEWWNDLWLNEGFAKFMEFVSVTVTHPELKVEDYFFGKCFNAMEVDALNSSHPVSTPVENPAQIREMFDDVSYEKGACILNMLRDYLSADTFKRGIVQYLQKYSYKNTKNEDLWNSMMHICPTDGTQTMDGFCSRSQHSSSTSHWRQEVVDVKTMMNTWTLQKGFPLITITVSGRNVHMKQEHYMKGSERFPETGYLWHVPLTFITSKSDSVQRFLLKTKTDVLILPEAVQWIKFNVGMNGYYIVHYADDGWASLSGLLKEAHTTISSNDRASLINNAFQLVSIEKLSIEKALDLTLYLKNETEIMPIFQALNELIPMYKLMEKRDMIEVETQFKDFLLKLLKDLIDKQTWTDEGSVSERMLRSQLLLLACVRNYQPCVQRAERYFREWKSSNGNMSIPIDVTLAVFAVGAQNTEGWDFLYSKYQSSLSSTEKSQIEFSLCTSKDPEKLQWLLDQSFKGEIIKTQEFPHILTLIGRNPVGYPLAWKFLRENWNKLVQKFELGSSSIAHMVMGTTDQFSTRARLEEVKGFFSSLKENGSQLRCVQQTIETIEENIRWMDKNFDKIRLWLQKEKPELL

Target ORF information:

RefSeq Version	XM_006517476.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus endoplasmic reticulum aminopeptidase 1 (Erap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517476.4

ORF Insert Sequence:

ATGCCCTCTC TTCTTCCCCT AGTATTGACA TTTTTATCTG TGTCATCTCC TTCTTGGTGT 
CAGAACAGTG ATATAGAATC TCTAAAAGCT AGTAATGGAG ACTCATTCCC TTGGAATAAT 
ATGCGACTTC CTGAGTATAT GACCCCGATT CATTATGATC TCATGATCCA TGCAAACCTC 
AGCACTCTGA CTTTCTGGGG AAAAACAGAA GTAGAAATCA TAGCTAGCCG GCCCACCAGC 
ACCATTATTA TGCATAGTCA CCACCTGCAA ATATCTAAGG CCACCCTCAG AAGAGGAGCT 
GGAGAGATGC TGTCTGAAGA ACCACTGAAG GTCCTGGAAT ATCCTGCTCA TGAGCAAGTT 
GCACTGCTGG CTGCCCAGCC GCTTCTTGCT GGGTCCCTGT ACACAGTTAT CATCGACTAT 
GCTGCCAACC TGTCTGAGAG TTTCCATGGA TTCTATAAGA GCACCTACAG AACACAGGAA 
GGTGAAATGA GAATACTTGC AGCAACACAG TTTGAACCCA CAGCTGCTAG AATGGCTTTT 
CCCTGCTTTG ATGAACCTGC CCTCAAGGCA AGTTTTTCCA TCAAGATAAA GAGGGATCCA 
AGGCACCTGG CCATCTCCAA CATGCCCCTG GTGAAATCTG TGAATGTTGC TGAAGGACTC 
ATAGAAGACC ATTTTGACAT CACTGTGAAG ATGAGTACCT ACCTAGTGGC CTTCATCATT 
TCTGATTTTA AGTCTGTGAG CAAGATGACT AAGAGTGGAG TCAAGGTTTC TGTGTATGCT 
GTGCCAGACA AGATAAATCA AGCCGATTAT GCTCTGGATG CTGCAGTGAC TCTTCTAGAG 
TTTTATGAGG ATTATTTCAA CATTCCATAT CCTTTACCCA AGCAAGATCT TGCTGCAATC 
CCAGACTTTC AGTCTGGTGC TATGGAAAAC TGGGGACTGA CCACATACAG AGAGTCCTCT 
CTGTTATACG ATAAAGAAAA GTCTTCTGCA TCTAGTAAGC TTGGCATCAC AATGATTGTG 
TCCCATGAAC TGGCTCACCA GTGGTTCGGG AACCTGGTCA CCATGGAATG GTGGAATGAC 
CTTTGGCTCA ATGAAGGATT TGCCAAATTT ATGGAGTTTG TGTCTGTCAC TGTGACCCAT 
CCTGAACTAA AAGTTGAAGA CTATTTCTTT GGCAAGTGTT TTAATGCAAT GGAAGTTGAT 
GCATTAAACT CCTCTCACCC TGTATCCACA CCTGTGGAGA ATCCTGCGCA GATTCGGGAG 
ATGTTTGATG ATGTTTCTTA TGAAAAGGGA GCTTGTATTC TGAATATGCT AAGAGATTAT 
CTGAGTGCAG ATACATTTAA AAGAGGAATT GTACAGTATC TCCAAAAGTA TAGTTATAAA 
AACACAAAAA ACGAGGACCT GTGGAATAGC ATGATGCATA TTTGCCCTAC AGATGGCACA 
CAAACAATGG ATGGCTTCTG CTCTAGAAGC CAACACTCAT CATCAACCTC ACACTGGCGT 
CAGGAGGTTG TAGATGTAAA GACCATGATG AACACATGGA CACTGCAGAA GGGCTTTCCT 
CTGATAACCA TCACTGTGAG TGGGCGGAAT GTGCACATGA AGCAAGAACA CTACATGAAG 
GGTTCAGAAC GCTTCCCAGA GACTGGGTAT TTGTGGCATG TTCCACTGAC ATTCATTACC 
AGCAAATCAG ACTCAGTCCA GAGATTTTTG CTGAAGACAA AGACAGATGT GCTCATCCTC 
CCAGAAGCAG TGCAGTGGAT CAAATTTAAC GTGGGAATGA ATGGCTATTA CATTGTGCAT 
TACGCGGATG ATGGATGGGC TTCTCTGAGT GGCCTTTTAA AAGAAGCGCA CACAACAATC 
AGCAGTAATG ATCGGGCAAG TCTCATCAAC AATGCATTTC AGCTGGTCAG CATTGAAAAG 
TTATCAATAG AAAAAGCCCT GGATTTAACC CTGTACTTGA AAAATGAAAC TGAAATTATG 
CCCATATTCC AAGCTTTGAA TGAACTCATA CCTATGTATA AGTTAATGGA GAAAAGAGAT 
ATGATTGAGG TGGAAACTCA ATTTAAGGAC TTCCTTCTCA AGTTGCTGAA GGACCTTATT 
GATAAGCAGA CTTGGACAGA TGAAGGCTCT GTCTCAGAGA GGATGCTCAG GAGCCAGCTC 
CTCCTCCTCG CATGTGTGCG CAATTATCAG CCCTGTGTGC AGAGGGCAGA GCGCTATTTC 
AGAGAGTGGA AGTCATCTAA TGGAAACATG AGCATCCCTA TTGATGTGAC CTTGGCTGTG 
TTTGCTGTCG GGGCCCAAAA CACAGAAGGC TGGGATTTTC TTTATAGTAA ATATCAGTCT 
TCTTTGTCTA GTACTGAGAA AAGCCAAATT GAATTTTCCC TCTGTACAAG CAAAGATCCA 
GAAAAACTTC AGTGGCTTCT AGATCAAAGT TTTAAGGGAG AGATAATAAA AACTCAGGAA 
TTTCCACATA TTCTCACACT CATTGGCAGA AACCCAGTGG GTTATCCATT GGCCTGGAAG 
TTTCTGAGGG AAAATTGGAA TAAGCTTGTA CAGAAATTTG AACTTGGCTC ATCGTCCATA 
GCTCACATGG TAATGGGAAC AACAGACCAG TTTTCCACCA GAGCACGTCT TGAAGAGGTA 
AAAGGATTCT TCAGTTCCTT GAAAGAAAAC GGTTCTCAGC TCCGTTGTGT TCAACAAACC 
ATTGAGACCA TTGAAGAAAA CATACGATGG ATGGATAAGA ATTTTGATAA AATAAGACTG 
TGGCTGCAAA AAGAAAAGCC AGAGTTGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Erap1 ( XM_030247471.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103331.1 Mus musculus endoplasmic reticulum aminopeptidase 1 (Erap1), transcript variant X2, mRNA.

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CloneID	OMu86310
Clone ID Related Accession (Same CDS sequence)	XM_030247471.1
Accession Version	XM_030247471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	endoplasmic reticulum aminopeptidase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTC TTCTTCCCCT AGTATTGACA TTTTTATCTG TGTCATCTCC TTCTTGGTGT 
CAGAACAGTG ATATAGAATC TCTAAAAGCT AGTAATGGAG ACTCATTCCC TTGGAATAAT 
ATGCGACTTC CTGAGTATAT GACCCCGATT CATTATGATC TCATGATCCA TGCAAACCTC 
AGCACTCTGA CTTTCTGGGG AAAAACAGAA GTAGAAATCA TAGCTAGCCG GCCCACCAGC 
ACCATTATTA TGCATAGTCA CCACCTGCAA ATATCTAAGG CCACCCTCAG AAGAGGAGCT 
GGAGAGATGC TGTCTGAAGA ACCACTGAAG GTCCTGGAAT ATCCTGCTCA TGAGCAAGTT 
GCACTGCTGG CTGCCCAGCC GCTTCTTGCT GGGTCCCTGT ACACAGTTAT CATCGACTAT 
GCTGCCAACC TGTCTGAGAG TTTCCATGGA TTCTATAAGA GCACCTACAG AACACAGGAA 
GGTGAAATGA GAATACTTGC AGCAACACAG TTTGAACCCA CAGCTGCTAG AATGGCTTTT 
CCCTGCTTTG ATGAACCTGC CCTCAAGGCA AGTTTTTCCA TCAAGATAAA GAGGGATCCA 
AGGCACCTGG CCATCTCCAA CATGCCCCTG GTGAAATCTG TGAATGTTGC TGAAGGACTC 
ATAGAAGACC ATTTTGACAT CACTGTGAAG ATGAGTACCT ACCTAGTGGC CTTCATCATT 
TCTGATTTTA AGTCTGTGAG CAAGATGACT AAGAGTGGAG TCAAGGTTTC TGTGTATGCT 
GTGCCAGACA AGATAAATCA AGCCGATTAT GCTCTGGATG CTGCAGTGAC TCTTCTAGAG 
TTTTATGAGG ATTATTTCAA CATTCCATAT CCTTTACCCA AGCAAGATCT TGCTGCAATC 
CCAGACTTTC AGTCTGGTGC TATGGAAAAC TGGGGACTGA CCACATACAG AGAGTCCTCT 
CTGTTATACG ATAAAGAAAA GTCTTCTGCA TCTAGTAAGC TTGGCATCAC AATGATTGTG 
TCCCATGAAC TGGCTCACCA GTGGTTCGGG AACCTGGTCA CCATGGAATG GTGGAATGAC 
CTTTGGCTCA ATGAAGGATT TGCCAAATTT ATGGAGTTTG TGTCTGTCAC TGTGACCCAT 
CCTGAACTAA AAGTTGAAGA CTATTTCTTT GGCAAGTGTT TTAATGCAAT GGAAGTTGAT 
GCATTAAACT CCTCTCACCC TGTATCCACA CCTGTGGAGA ATCCTGCGCA GATTCGGGAG 
ATGTTTGATG ATGTTTCTTA TGAAAAGGGA GCTTGTATTC TGAATATGCT AAGAGATTAT 
CTGAGTGCAG ATACATTTAA AAGAGGAATT GTACAGTATC TCCAAAAGTA TAGTTATAAA 
AACACAAAAA ACGAGGACCT GTGGAATAGC ATGATGCATA TTTGCCCTAC AGATGGCACA 
CAAACAATGG ATGGCTTCTG CTCTAGAAGC CAACACTCAT CATCAACCTC ACACTGGCGT 
CAGGAGGTTG TAGATGTAAA GACCATGATG AACACATGGA CACTGCAGAA GGGCTTTCCT 
CTGATAACCA TCACTGTGAG TGGGCGGAAT GTGCACATGA AGCAAGAACA CTACATGAAG 
GGTTCAGAAC GCTTCCCAGA GACTGGGTAT TTGTGGCATG TTCCACTGAC ATTCATTACC 
AGCAAATCAG ACTCAGTCCA GAGATTTTTG CTGAAGACAA AGACAGATGT GCTCATCCTC 
CCAGAAGCAG TGCAGTGGAT CAAATTTAAC GTGGGAATGA ATGGCTATTA CATTGTGCAT 
TACGCGGATG ATGGATGGGC TTCTCTGAGT GGCCTTTTAA AAGAAGCGCA CACAACAATC 
AGCAGTAATG ATCGGGCAAG TCTCATCAAC AATGCATTTC AGCTGGTCAG CATTGAAAAG 
TTATCAATAG AAAAAGCCCT GGATTTAACC CTGTACTTGA AAAATGAAAC TGAAATTATG 
CCCATATTCC AAGCTTTGAA TGAACTCATA CCTATGTATA AGTTAATGGA GAAAAGAGAT 
ATGATTGAGG TGGAAACTCA ATTTAAGGAC TTCCTTCTCA AGTTGCTGAA GGACCTTATT 
GATAAGCAGA CTTGGACAGA TGAAGGCTCT GTCTCAGAGA GGATGCTCAG GAGCCAGCTC 
CTCCTCCTCG CATGTGTGCG CAATTATCAG CCCTGTGTGC AGAGGGCAGA GCGCTATTTC 
AGAGAGTGGA AGTCATCTAA TGGAAACATG AGCATCCCTA TTGATGTGAC CTTGGCTGTG 
TTTGCTGTCG GGGCCCAAAA CACAGAAGGC TGGGATTTTC TTTATAGTAA ATATCAGTCT 
TCTTTGTCTA GTACTGAGAA AAGCCAAATT GAATTTTCCC TCTGTACAAG CAAAGATCCA 
GAAAAACTTC AGTGGCTTCT AGATCAAAGT TTTAAGGGAG AGATAATAAA AACTCAGGAA 
TTTCCACATA TTCTCACACT CATTGGCAGA AACCCAGTGG GTTATCCATT GGCCTGGAAG 
TTTCTGAGGG AAAATTGGAA TAAGCTTGTA CAGAAATTTG AACTTGGCTC ATCGTCCATA 
GCTCACATGG TAATGGGAAC AACAGACCAG TTTTCCACCA GAGCACGTCT TGAAGAGGTA 
AAGGGGTGTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103331.1
CDS	375..3029
Translation	MPSLLPLVLTFLSVSSPSWCQNSDIESLKASNGDSFPWNNMRLPEYMTPIHYDLMIHANLSTLTFWGKTEVEIIASRPTSTIIMHSHHLQISKATLRRGAGEMLSEEPLKVLEYPAHEQVALLAAQPLLAGSLYTVIIDYAANLSESFHGFYKSTYRTQEGEMRILAATQFEPTAARMAFPCFDEPALKASFSIKIKRDPRHLAISNMPLVKSVNVAEGLIEDHFDITVKMSTYLVAFIISDFKSVSKMTKSGVKVSVYAVPDKINQADYALDAAVTLLEFYEDYFNIPYPLPKQDLAAIPDFQSGAMENWGLTTYRESSLLYDKEKSSASSKLGITMIVSHELAHQWFGNLVTMEWWNDLWLNEGFAKFMEFVSVTVTHPELKVEDYFFGKCFNAMEVDALNSSHPVSTPVENPAQIREMFDDVSYEKGACILNMLRDYLSADTFKRGIVQYLQKYSYKNTKNEDLWNSMMHICPTDGTQTMDGFCSRSQHSSSTSHWRQEVVDVKTMMNTWTLQKGFPLITITVSGRNVHMKQEHYMKGSERFPETGYLWHVPLTFITSKSDSVQRFLLKTKTDVLILPEAVQWIKFNVGMNGYYIVHYADDGWASLSGLLKEAHTTISSNDRASLINNAFQLVSIEKLSIEKALDLTLYLKNETEIMPIFQALNELIPMYKLMEKRDMIEVETQFKDFLLKLLKDLIDKQTWTDEGSVSERMLRSQLLLLACVRNYQPCVQRAERYFREWKSSNGNMSIPIDVTLAVFAVGAQNTEGWDFLYSKYQSSLSSTEKSQIEFSLCTSKDPEKLQWLLDQSFKGEIIKTQEFPHILTLIGRNPVGYPLAWKFLRENWNKLVQKFELGSSSIAHMVMGTTDQFSTRARLEEVKGCV

Target ORF information:

RefSeq Version	XM_030247471.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus endoplasmic reticulum aminopeptidase 1 (Erap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247471.1

ORF Insert Sequence:

ATGCCCTCTC TTCTTCCCCT AGTATTGACA TTTTTATCTG TGTCATCTCC TTCTTGGTGT 
CAGAACAGTG ATATAGAATC TCTAAAAGCT AGTAATGGAG ACTCATTCCC TTGGAATAAT 
ATGCGACTTC CTGAGTATAT GACCCCGATT CATTATGATC TCATGATCCA TGCAAACCTC 
AGCACTCTGA CTTTCTGGGG AAAAACAGAA GTAGAAATCA TAGCTAGCCG GCCCACCAGC 
ACCATTATTA TGCATAGTCA CCACCTGCAA ATATCTAAGG CCACCCTCAG AAGAGGAGCT 
GGAGAGATGC TGTCTGAAGA ACCACTGAAG GTCCTGGAAT ATCCTGCTCA TGAGCAAGTT 
GCACTGCTGG CTGCCCAGCC GCTTCTTGCT GGGTCCCTGT ACACAGTTAT CATCGACTAT 
GCTGCCAACC TGTCTGAGAG TTTCCATGGA TTCTATAAGA GCACCTACAG AACACAGGAA 
GGTGAAATGA GAATACTTGC AGCAACACAG TTTGAACCCA CAGCTGCTAG AATGGCTTTT 
CCCTGCTTTG ATGAACCTGC CCTCAAGGCA AGTTTTTCCA TCAAGATAAA GAGGGATCCA 
AGGCACCTGG CCATCTCCAA CATGCCCCTG GTGAAATCTG TGAATGTTGC TGAAGGACTC 
ATAGAAGACC ATTTTGACAT CACTGTGAAG ATGAGTACCT ACCTAGTGGC CTTCATCATT 
TCTGATTTTA AGTCTGTGAG CAAGATGACT AAGAGTGGAG TCAAGGTTTC TGTGTATGCT 
GTGCCAGACA AGATAAATCA AGCCGATTAT GCTCTGGATG CTGCAGTGAC TCTTCTAGAG 
TTTTATGAGG ATTATTTCAA CATTCCATAT CCTTTACCCA AGCAAGATCT TGCTGCAATC 
CCAGACTTTC AGTCTGGTGC TATGGAAAAC TGGGGACTGA CCACATACAG AGAGTCCTCT 
CTGTTATACG ATAAAGAAAA GTCTTCTGCA TCTAGTAAGC TTGGCATCAC AATGATTGTG 
TCCCATGAAC TGGCTCACCA GTGGTTCGGG AACCTGGTCA CCATGGAATG GTGGAATGAC 
CTTTGGCTCA ATGAAGGATT TGCCAAATTT ATGGAGTTTG TGTCTGTCAC TGTGACCCAT 
CCTGAACTAA AAGTTGAAGA CTATTTCTTT GGCAAGTGTT TTAATGCAAT GGAAGTTGAT 
GCATTAAACT CCTCTCACCC TGTATCCACA CCTGTGGAGA ATCCTGCGCA GATTCGGGAG 
ATGTTTGATG ATGTTTCTTA TGAAAAGGGA GCTTGTATTC TGAATATGCT AAGAGATTAT 
CTGAGTGCAG ATACATTTAA AAGAGGAATT GTACAGTATC TCCAAAAGTA TAGTTATAAA 
AACACAAAAA ACGAGGACCT GTGGAATAGC ATGATGCATA TTTGCCCTAC AGATGGCACA 
CAAACAATGG ATGGCTTCTG CTCTAGAAGC CAACACTCAT CATCAACCTC ACACTGGCGT 
CAGGAGGTTG TAGATGTAAA GACCATGATG AACACATGGA CACTGCAGAA GGGCTTTCCT 
CTGATAACCA TCACTGTGAG TGGGCGGAAT GTGCACATGA AGCAAGAACA CTACATGAAG 
GGTTCAGAAC GCTTCCCAGA GACTGGGTAT TTGTGGCATG TTCCACTGAC ATTCATTACC 
AGCAAATCAG ACTCAGTCCA GAGATTTTTG CTGAAGACAA AGACAGATGT GCTCATCCTC 
CCAGAAGCAG TGCAGTGGAT CAAATTTAAC GTGGGAATGA ATGGCTATTA CATTGTGCAT 
TACGCGGATG ATGGATGGGC TTCTCTGAGT GGCCTTTTAA AAGAAGCGCA CACAACAATC 
AGCAGTAATG ATCGGGCAAG TCTCATCAAC AATGCATTTC AGCTGGTCAG CATTGAAAAG 
TTATCAATAG AAAAAGCCCT GGATTTAACC CTGTACTTGA AAAATGAAAC TGAAATTATG 
CCCATATTCC AAGCTTTGAA TGAACTCATA CCTATGTATA AGTTAATGGA GAAAAGAGAT 
ATGATTGAGG TGGAAACTCA ATTTAAGGAC TTCCTTCTCA AGTTGCTGAA GGACCTTATT 
GATAAGCAGA CTTGGACAGA TGAAGGCTCT GTCTCAGAGA GGATGCTCAG GAGCCAGCTC 
CTCCTCCTCG CATGTGTGCG CAATTATCAG CCCTGTGTGC AGAGGGCAGA GCGCTATTTC 
AGAGAGTGGA AGTCATCTAA TGGAAACATG AGCATCCCTA TTGATGTGAC CTTGGCTGTG 
TTTGCTGTCG GGGCCCAAAA CACAGAAGGC TGGGATTTTC TTTATAGTAA ATATCAGTCT 
TCTTTGTCTA GTACTGAGAA AAGCCAAATT GAATTTTCCC TCTGTACAAG CAAAGATCCA 
GAAAAACTTC AGTGGCTTCT AGATCAAAGT TTTAAGGGAG AGATAATAAA AACTCAGGAA 
TTTCCACATA TTCTCACACT CATTGGCAGA AACCCAGTGG GTTATCCATT GGCCTGGAAG 
TTTCTGAGGG AAAATTGGAA TAAGCTTGTA CAGAAATTTG AACTTGGCTC ATCGTCCATA 
GCTCACATGG TAATGGGAAC AACAGACCAG TTTTCCACCA GAGCACGTCT TGAAGAGGTA 
AAGGGGTGTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mice expressing human ERAP1 variants associated with ankylosing spondylitis have altered T-cell repertoires and NK cell functions, as well as increased in utero and perinatal mortality.
International immunology29(6)277-289(2017 Jun)
Rastall DPW,Alyaquob FS,O'Connell P,Pepelyayeva Y,Peters D,Godbehere-Roosa S,Pereira-Hicks C,Aldhamen YA,Amalfitano A

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Erap1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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