Nfkbiz cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nfkbiz
Entrez Gene ID	80859
Full Name	nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta
Synonyms	AA408868,INAP,Mail
General protein information	Preferred Names nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta Names NF-kappa-B inhibitor zeta I-kappa-B-zeta IL-1 inducible nuclear ankyrin-repeat protein IkappaB-zeta ikB-zeta ikappaBzeta molecule possessing ankyrin-repeats induced by lipopolysaccharide
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 C1.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_030249339.1	XP_030105199.1	NF-kappa-B inhibitor zeta isoform X1
NM_001159394.1	NP_001152866.1	NF-kappa-B inhibitor zeta isoform a
NM_001159395.1	NP_001152867.1	NF-kappa-B inhibitor zeta isoform b
NM_030612.3	NP_085115.1	NF-kappa-B inhibitor zeta isoform a

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NFKBIZ	XP_005639490.1
Gallus gallus (chicken)	NFKBIZ	NP_001006254.1
Xenopus tropicalis (tropical clawed frog)	LOC100485153	XP_002932322.1
Pan troglodytes (chimpanzee)	NFKBIZ	XP_516633.3
Mus musculus (house mouse)	Nfkbiz	NP_001152866.1
Bos taurus (cattle)	NFKBIZ	NP_777151.1
Rattus norvegicus (Norway rat)	Nfkbiz	NP_001100565.1
Xenopus tropicalis (tropical clawed frog)	nfkbiz	NP_001123738.1
Homo sapiens (human)	NFKBIZ	NP_113607.1
	NFKBIZ	XP_001099425.1

Gene Ontology
Bibliography

Related articles in PubMed

The resident pathobiont Staphylococcus xylosus in Nfkbiz-deficient skin accelerates spontaneous skin inflammation.
Kim Y, Lee YS, Yang JY, Lee SH, Park YY, Kweon MN
Scientific reports7(1)6348(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In cultured macrophages, LPS-induced expression of monocyte chemoattractant protein (MCP)-1 was suppressed by NaN3 through inhibition of STAT1 and IkappaBzeta activities.
Title: Sodium azide suppresses LPS-induced expression MCP-1 through regulating I?B? and STAT1 activities in macrophages.

Data show that I-kappa-B-zeta (IkappaBzeta) regulates macrophage interleukin-10 (IL_10) expression by directly activated the Il-10 promoter.
Title: The Atypical Inhibitor of NF-?B, I?B?, Controls Macrophage Interleukin-10 Expression.

These data reveal a previously unappreciated role for IkappaB-zeta in IFN-gamma production in T cells and the immunoregulatory function of T regulatory cells.
Title: Control of IFN-? production and regulatory function by the inducible nuclear protein I?B-? in T cells.

Nfkbiz-/- keratinocytes were hypoproliferative. In skin from Nfkbiz-/- mice, the expression of the keratinocyte differentiation markers K10 and filaggrin were reduced, although that of K14 was unchanged.
Title: Nfkbiz regulates the proliferation and differentiation of keratinocytes.

A possible important role of Nfkbiz in modulating the progression of inflammatory diseases in which IFN-gamma and IL-1 are abundant.
Title: The role of IFN-? in regulating Nfkbiz expression in epidermal keratinocytes.

The following Nfkbiz gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nfkbiz cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07434	NM_001159394.1 Latest version!	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta (Nfkbiz), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu07434	NM_030612.3 Latest version!	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta (Nfkbiz), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu07437	NM_001159395.1 Latest version!	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta (Nfkbiz), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu07434	XM_030249339.1 Latest version!	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta (Nfkbiz), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nfkbiz ( NM_001159394.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001152866.1 Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta (Nfkbiz), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07434
Clone ID Related Accession (Same CDS sequence)	XM_030249339.1 , NM_001159394.1 , NM_030612.3
Accession Version	NM_001159394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	NF-kappa-B inhibitor zeta isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB026551.2 and AK082908.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB026551.2, AK159917.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCGTGG ACAAGCTGCT GGACGACAGC CGCGGCGGAG AGGGGCTGCT GGACGCGGCC 
GGCGACTGCG GCCTCATGAC CAGCCCGCTC AACCTGGCTT ACTTCTACGG CGCCTCGCCT 
CCTTCCGCCC CCGGCGCCGG CGACACCGGC TACCTGTCCG CCGTGCCCTC CGCGCCCGGC 
TCGCCCGGCT CCGACTCCTC CGATTTCTCC TCCACTTCCT CCGTGTCCTC CTGCGGGGCC 
GTGGAGTCCC GTCCCAGAGG TGGCGCCCGC GCCGAGCGCC CTCAAGTTGA GCCCCACATG 
GGGGTTGGCA GGCAGCAGAG AGGCCCCTTT CAAGGTGTTC GCGTGAAGAA CTCGGTGAAG 
GAGCTCCTGC TACACATCCG AAGCAACAAG CAGAAGGCTT CCGGCCAGCC CGTGGATGAG 
TTTAAGACAC AGAGTGTGAA CATTGAGCAG CTGACAGACC TGAAGAGTGC AGTGTCCGCA 
GTGGGGAAAA GAAAGGGACC CGATCCTCTG TCCGATGGAC CGGTTTGCAA GAGGCCAGCT 
CTGTTGCCTT CTCACTTCGT GACATCACCG CAAACGCCTA CACCTGGGGA GAGCATGGAA 
GATGTTCGCC ACAGCGAATC CAAGCTGGAT AGCAGCGCTG CCCTGCTTCA GAATATTATA 
AACATCAAGA ATGAGTGCAA CCCAGTTTCA CTAAACACTG TTCAGGTTAG CTGGATGAGC 
CCCACGGTTC CCCAGAACTC GCCAAGAGAC CAGTGCCAGG ATTTCCATGG AGGCCAGGCC 
TTCTCTCCAC CTCAGAAATA CCAACCGTTC CAAGTCAGTG GCTCTCCACA GATGATGGAC 
CAGGCGTCAA TGTACCAGTA TTCGCCACAA ACACAGAACA TGCAGCAGCC GCCGCCGCTG 
CCACCACAGC AGCAACATCA ACAGAATTAT CCCCACAACT CACCCCTGCA GTTCAGTCCT 
TACTCCAGAA TGTCCCAGTC TCCCAAGTAT GATTCCAACC TCTTTGATAC TCACGAGCCA 
CAGTTCTGCA CGGGCCAAAG TTTTGTGTCT TTACTGACTG GCCCGGGGGA ACCCGAGTCT 
CTTGCTGTTC CCGTTCCGGC TCCCACCAGC ATTCCACCCC AAACTGAGAC TCAGCTGCAG 
ACCTTCAGCC TGATGCCAAG CAATGCCTGC GAGGCCGTCG TCGGGGTGCA CGATGTGGGC 
TCGCACTCTT TGGGCACGTC CCTGTCACTC CAGAACATCA TGGGAAGCCC GATGAATACC 
ACCCAGCTGG GGAAGTCATT TTTTCAGTGG CAGGTAGAGC AGGAAGAAAG CAAATTGGCA 
AATATTCCCC AGGACCAGTT CCTCGCTAGG GACGGAGATG GTGACACGTT TCTCCATATT 
GCTGTTGCCC AAGGGAGGCG AGCGCTCTCG TATGTCCTTG CAAGAAAGAT GAACGCTCTT 
CACATGCTGG ACATTAAGGA GCACAATGGA CAGAGTGCCT TTCAGGTGGC GGTAGCTGCC 
AATCAGCACC TCATTGTGCA AGATCTGGTA AACCTTGGTG CCCAGGTGAA CACCACGGAT 
TGCTGGGGAA GGACACCTCT GCATGTCTGT GCGGAGAAGG GCCACTCCCA GGTGCTCCAG 
GCCATCCAGA AGGGAGCTGT GAGGAGCAAT CAGTTTGTGG ATCTTGAGGC AACGAACTAT 
GATGGCCTGA CTCCCCTACA TTGCGCGGTC GTGGCTCACA ATGCGGTGGT GCACGAACTG 
CAGAGGAATC GGCAGTCTCA TTCGCCCGAA GTGCAGGACC TCTTACTAAG GAATAAGAGC 
CTGGTAGACA CCATTAAGTG TCTGATTCAG ATGGGAGCCG CAGTGGAGGC AAAGGATCGT 
AAGAGTGGCC GCACAGCCCT GCACTTGGCA GCGGAAGAAG CAAATCTGGA ACTCATTCGC 
CTCTTTTTGG AGTTGCCCAG TTGCCTGTCT TTCGTGAATG CCAAGGCTTA CAATGGAAAC 
ACCGCCCTCC ATGTTGCTGC CAGCCTGCAG TATCGGGTGA CACAGTTGGA CGCAGTCCGC 
CTGTTGATGA GGAAGGGAGC TGACCCCAGT ACTCGGAACT TGGAGAATGA ACAGCCCGTG 
CATTTGGTCC CCGATGGCCC AGTGGGAGAA CAGATCCGAC GTATCCTGAA GGGGAAGTCC 
ATTCAGCAGC GAGCACCACC ATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001152866.1
CDS	86..2272
Translation	MIVDKLLDDSRGGEGLLDAAGDCGLMTSPLNLAYFYGASPPSAPGAGDTGYLSAVPSAPGSPGSDSSDFSSTSSVSSCGAVESRPRGGARAERPQVEPHMGVGRQQRGPFQGVRVKNSVKELLLHIRSNKQKASGQPVDEFKTQSVNIEQLTDLKSAVSAVGKRKGPDPLSDGPVCKRPALLPSHFVTSPQTPTPGESMEDVRHSESKLDSSAALLQNIINIKNECNPVSLNTVQVSWMSPTVPQNSPRDQCQDFHGGQAFSPPQKYQPFQVSGSPQMMDQASMYQYSPQTQNMQQPPPLPPQQQHQQNYPHNSPLQFSPYSRMSQSPKYDSNLFDTHEPQFCTGQSFVSLLTGPGEPESLAVPVPAPTSIPPQTETQLQTFSLMPSNACEAVVGVHDVGSHSLGTSLSLQNIMGSPMNTTQLGKSFFQWQVEQEESKLANIPQDQFLARDGDGDTFLHIAVAQGRRALSYVLARKMNALHMLDIKEHNGQSAFQVAVAANQHLIVQDLVNLGAQVNTTDCWGRTPLHVCAEKGHSQVLQAIQKGAVRSNQFVDLEATNYDGLTPLHCAVVAHNAVVHELQRNRQSHSPEVQDLLLRNKSLVDTIKCLIQMGAAVEAKDRKSGRTALHLAAEEANLELIRLFLELPSCLSFVNAKAYNGNTALHVAASLQYRVTQLDAVRLLMRKGADPSTRNLENEQPVHLVPDGPVGEQIRRILKGKSIQQRAPPY

Target ORF information:

RefSeq Version	NM_001159394.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta (Nfkbiz), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159394.1

ORF Insert Sequence:

ATGATCGTGG ACAAGCTGCT GGACGACAGC CGCGGCGGAG AGGGGCTGCT GGACGCGGCC 
GGCGACTGCG GCCTCATGAC CAGCCCGCTC AACCTGGCTT ACTTCTACGG CGCCTCGCCT 
CCTTCCGCCC CCGGCGCCGG CGACACCGGC TACCTGTCCG CCGTGCCCTC CGCGCCCGGC 
TCGCCCGGCT CCGACTCCTC CGATTTCTCC TCCACTTCCT CCGTGTCCTC CTGCGGGGCC 
GTGGAGTCCC GTCCCAGAGG TGGCGCCCGC GCCGAGCGCC CTCAAGTTGA GCCCCACATG 
GGGGTTGGCA GGCAGCAGAG AGGCCCCTTT CAAGGTGTTC GCGTGAAGAA CTCGGTGAAG 
GAGCTCCTGC TACACATCCG AAGCAACAAG CAGAAGGCTT CCGGCCAGCC CGTGGATGAG 
TTTAAGACAC AGAGTGTGAA CATTGAGCAG CTGACAGACC TGAAGAGTGC AGTGTCCGCA 
GTGGGGAAAA GAAAGGGACC CGATCCTCTG TCCGATGGAC CGGTTTGCAA GAGGCCAGCT 
CTGTTGCCTT CTCACTTCGT GACATCACCG CAAACGCCTA CACCTGGGGA GAGCATGGAA 
GATGTTCGCC ACAGCGAATC CAAGCTGGAT AGCAGCGCTG CCCTGCTTCA GAATATTATA 
AACATCAAGA ATGAGTGCAA CCCAGTTTCA CTAAACACTG TTCAGGTTAG CTGGATGAGC 
CCCACGGTTC CCCAGAACTC GCCAAGAGAC CAGTGCCAGG ATTTCCATGG AGGCCAGGCC 
TTCTCTCCAC CTCAGAAATA CCAACCGTTC CAAGTCAGTG GCTCTCCACA GATGATGGAC 
CAGGCGTCAA TGTACCAGTA TTCGCCACAA ACACAGAACA TGCAGCAGCC GCCGCCGCTG 
CCACCACAGC AGCAACATCA ACAGAATTAT CCCCACAACT CACCCCTGCA GTTCAGTCCT 
TACTCCAGAA TGTCCCAGTC TCCCAAGTAT GATTCCAACC TCTTTGATAC TCACGAGCCA 
CAGTTCTGCA CGGGCCAAAG TTTTGTGTCT TTACTGACTG GCCCGGGGGA ACCCGAGTCT 
CTTGCTGTTC CCGTTCCGGC TCCCACCAGC ATTCCACCCC AAACTGAGAC TCAGCTGCAG 
ACCTTCAGCC TGATGCCAAG CAATGCCTGC GAGGCCGTCG TCGGGGTGCA CGATGTGGGC 
TCGCACTCTT TGGGCACGTC CCTGTCACTC CAGAACATCA TGGGAAGCCC GATGAATACC 
ACCCAGCTGG GGAAGTCATT TTTTCAGTGG CAGGTAGAGC AGGAAGAAAG CAAATTGGCA 
AATATTCCCC AGGACCAGTT CCTCGCTAGG GACGGAGATG GTGACACGTT TCTCCATATT 
GCTGTTGCCC AAGGGAGGCG AGCGCTCTCG TATGTCCTTG CAAGAAAGAT GAACGCTCTT 
CACATGCTGG ACATTAAGGA GCACAATGGA CAGAGTGCCT TTCAGGTGGC GGTAGCTGCC 
AATCAGCACC TCATTGTGCA AGATCTGGTA AACCTTGGTG CCCAGGTGAA CACCACGGAT 
TGCTGGGGAA GGACACCTCT GCATGTCTGT GCGGAGAAGG GCCACTCCCA GGTGCTCCAG 
GCCATCCAGA AGGGAGCTGT GAGGAGCAAT CAGTTTGTGG ATCTTGAGGC AACGAACTAT 
GATGGCCTGA CTCCCCTACA TTGCGCGGTC GTGGCTCACA ATGCGGTGGT GCACGAACTG 
CAGAGGAATC GGCAGTCTCA TTCGCCCGAA GTGCAGGACC TCTTACTAAG GAATAAGAGC 
CTGGTAGACA CCATTAAGTG TCTGATTCAG ATGGGAGCCG CAGTGGAGGC AAAGGATCGT 
AAGAGTGGCC GCACAGCCCT GCACTTGGCA GCGGAAGAAG CAAATCTGGA ACTCATTCGC 
CTCTTTTTGG AGTTGCCCAG TTGCCTGTCT TTCGTGAATG CCAAGGCTTA CAATGGAAAC 
ACCGCCCTCC ATGTTGCTGC CAGCCTGCAG TATCGGGTGA CACAGTTGGA CGCAGTCCGC 
CTGTTGATGA GGAAGGGAGC TGACCCCAGT ACTCGGAACT TGGAGAATGA ACAGCCCGTG 
CATTTGGTCC CCGATGGCCC AGTGGGAGAA CAGATCCGAC GTATCCTGAA GGGGAAGTCC 
ATTCAGCAGC GAGCACCACC ATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfkbiz ( NM_030612.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_085115.1 Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta (Nfkbiz), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07434
Clone ID Related Accession (Same CDS sequence)	XM_030249339.1 , NM_001159394.1 , NM_030612.3
Accession Version	NM_030612.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	NF-kappa-B inhibitor zeta isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB047549.2, AB026551.2 and AK159171.1. On Apr 16, 2009 this sequence version replaced NM_030612.2. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 both encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB020974.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGATCGTGG ACAAGCTGCT GGACGACAGC CGCGGCGGAG AGGGGCTGCT GGACGCGGCC 
GGCGACTGCG GCCTCATGAC CAGCCCGCTC AACCTGGCTT ACTTCTACGG CGCCTCGCCT 
CCTTCCGCCC CCGGCGCCGG CGACACCGGC TACCTGTCCG CCGTGCCCTC CGCGCCCGGC 
TCGCCCGGCT CCGACTCCTC CGATTTCTCC TCCACTTCCT CCGTGTCCTC CTGCGGGGCC 
GTGGAGTCCC GTCCCAGAGG TGGCGCCCGC GCCGAGCGCC CTCAAGTTGA GCCCCACATG 
GGGGTTGGCA GGCAGCAGAG AGGCCCCTTT CAAGGTGTTC GCGTGAAGAA CTCGGTGAAG 
GAGCTCCTGC TACACATCCG AAGCAACAAG CAGAAGGCTT CCGGCCAGCC CGTGGATGAG 
TTTAAGACAC AGAGTGTGAA CATTGAGCAG CTGACAGACC TGAAGAGTGC AGTGTCCGCA 
GTGGGGAAAA GAAAGGGACC CGATCCTCTG TCCGATGGAC CGGTTTGCAA GAGGCCAGCT 
CTGTTGCCTT CTCACTTCGT GACATCACCG CAAACGCCTA CACCTGGGGA GAGCATGGAA 
GATGTTCGCC ACAGCGAATC CAAGCTGGAT AGCAGCGCTG CCCTGCTTCA GAATATTATA 
AACATCAAGA ATGAGTGCAA CCCAGTTTCA CTAAACACTG TTCAGGTTAG CTGGATGAGC 
CCCACGGTTC CCCAGAACTC GCCAAGAGAC CAGTGCCAGG ATTTCCATGG AGGCCAGGCC 
TTCTCTCCAC CTCAGAAATA CCAACCGTTC CAAGTCAGTG GCTCTCCACA GATGATGGAC 
CAGGCGTCAA TGTACCAGTA TTCGCCACAA ACACAGAACA TGCAGCAGCC GCCGCCGCTG 
CCACCACAGC AGCAACATCA ACAGAATTAT CCCCACAACT CACCCCTGCA GTTCAGTCCT 
TACTCCAGAA TGTCCCAGTC TCCCAAGTAT GATTCCAACC TCTTTGATAC TCACGAGCCA 
CAGTTCTGCA CGGGCCAAAG TTTTGTGTCT TTACTGACTG GCCCGGGGGA ACCCGAGTCT 
CTTGCTGTTC CCGTTCCGGC TCCCACCAGC ATTCCACCCC AAACTGAGAC TCAGCTGCAG 
ACCTTCAGCC TGATGCCAAG CAATGCCTGC GAGGCCGTCG TCGGGGTGCA CGATGTGGGC 
TCGCACTCTT TGGGCACGTC CCTGTCACTC CAGAACATCA TGGGAAGCCC GATGAATACC 
ACCCAGCTGG GGAAGTCATT TTTTCAGTGG CAGGTAGAGC AGGAAGAAAG CAAATTGGCA 
AATATTCCCC AGGACCAGTT CCTCGCTAGG GACGGAGATG GTGACACGTT TCTCCATATT 
GCTGTTGCCC AAGGGAGGCG AGCGCTCTCG TATGTCCTTG CAAGAAAGAT GAACGCTCTT 
CACATGCTGG ACATTAAGGA GCACAATGGA CAGAGTGCCT TTCAGGTGGC GGTAGCTGCC 
AATCAGCACC TCATTGTGCA AGATCTGGTA AACCTTGGTG CCCAGGTGAA CACCACGGAT 
TGCTGGGGAA GGACACCTCT GCATGTCTGT GCGGAGAAGG GCCACTCCCA GGTGCTCCAG 
GCCATCCAGA AGGGAGCTGT GAGGAGCAAT CAGTTTGTGG ATCTTGAGGC AACGAACTAT 
GATGGCCTGA CTCCCCTACA TTGCGCGGTC GTGGCTCACA ATGCGGTGGT GCACGAACTG 
CAGAGGAATC GGCAGTCTCA TTCGCCCGAA GTGCAGGACC TCTTACTAAG GAATAAGAGC 
CTGGTAGACA CCATTAAGTG TCTGATTCAG ATGGGAGCCG CAGTGGAGGC AAAGGATCGT 
AAGAGTGGCC GCACAGCCCT GCACTTGGCA GCGGAAGAAG CAAATCTGGA ACTCATTCGC 
CTCTTTTTGG AGTTGCCCAG TTGCCTGTCT TTCGTGAATG CCAAGGCTTA CAATGGAAAC 
ACCGCCCTCC ATGTTGCTGC CAGCCTGCAG TATCGGGTGA CACAGTTGGA CGCAGTCCGC 
CTGTTGATGA GGAAGGGAGC TGACCCCAGT ACTCGGAACT TGGAGAATGA ACAGCCCGTG 
CATTTGGTCC CCGATGGCCC AGTGGGAGAA CAGATCCGAC GTATCCTGAA GGGGAAGTCC 
ATTCAGCAGC GAGCACCACC ATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_085115.1
CDS	316..2502
Translation	MIVDKLLDDSRGGEGLLDAAGDCGLMTSPLNLAYFYGASPPSAPGAGDTGYLSAVPSAPGSPGSDSSDFSSTSSVSSCGAVESRPRGGARAERPQVEPHMGVGRQQRGPFQGVRVKNSVKELLLHIRSNKQKASGQPVDEFKTQSVNIEQLTDLKSAVSAVGKRKGPDPLSDGPVCKRPALLPSHFVTSPQTPTPGESMEDVRHSESKLDSSAALLQNIINIKNECNPVSLNTVQVSWMSPTVPQNSPRDQCQDFHGGQAFSPPQKYQPFQVSGSPQMMDQASMYQYSPQTQNMQQPPPLPPQQQHQQNYPHNSPLQFSPYSRMSQSPKYDSNLFDTHEPQFCTGQSFVSLLTGPGEPESLAVPVPAPTSIPPQTETQLQTFSLMPSNACEAVVGVHDVGSHSLGTSLSLQNIMGSPMNTTQLGKSFFQWQVEQEESKLANIPQDQFLARDGDGDTFLHIAVAQGRRALSYVLARKMNALHMLDIKEHNGQSAFQVAVAANQHLIVQDLVNLGAQVNTTDCWGRTPLHVCAEKGHSQVLQAIQKGAVRSNQFVDLEATNYDGLTPLHCAVVAHNAVVHELQRNRQSHSPEVQDLLLRNKSLVDTIKCLIQMGAAVEAKDRKSGRTALHLAAEEANLELIRLFLELPSCLSFVNAKAYNGNTALHVAASLQYRVTQLDAVRLLMRKGADPSTRNLENEQPVHLVPDGPVGEQIRRILKGKSIQQRAPPY

Target ORF information:

RefSeq Version	NM_030612.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta (Nfkbiz), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030612.3

ORF Insert Sequence:

ATGATCGTGG ACAAGCTGCT GGACGACAGC CGCGGCGGAG AGGGGCTGCT GGACGCGGCC 
GGCGACTGCG GCCTCATGAC CAGCCCGCTC AACCTGGCTT ACTTCTACGG CGCCTCGCCT 
CCTTCCGCCC CCGGCGCCGG CGACACCGGC TACCTGTCCG CCGTGCCCTC CGCGCCCGGC 
TCGCCCGGCT CCGACTCCTC CGATTTCTCC TCCACTTCCT CCGTGTCCTC CTGCGGGGCC 
GTGGAGTCCC GTCCCAGAGG TGGCGCCCGC GCCGAGCGCC CTCAAGTTGA GCCCCACATG 
GGGGTTGGCA GGCAGCAGAG AGGCCCCTTT CAAGGTGTTC GCGTGAAGAA CTCGGTGAAG 
GAGCTCCTGC TACACATCCG AAGCAACAAG CAGAAGGCTT CCGGCCAGCC CGTGGATGAG 
TTTAAGACAC AGAGTGTGAA CATTGAGCAG CTGACAGACC TGAAGAGTGC AGTGTCCGCA 
GTGGGGAAAA GAAAGGGACC CGATCCTCTG TCCGATGGAC CGGTTTGCAA GAGGCCAGCT 
CTGTTGCCTT CTCACTTCGT GACATCACCG CAAACGCCTA CACCTGGGGA GAGCATGGAA 
GATGTTCGCC ACAGCGAATC CAAGCTGGAT AGCAGCGCTG CCCTGCTTCA GAATATTATA 
AACATCAAGA ATGAGTGCAA CCCAGTTTCA CTAAACACTG TTCAGGTTAG CTGGATGAGC 
CCCACGGTTC CCCAGAACTC GCCAAGAGAC CAGTGCCAGG ATTTCCATGG AGGCCAGGCC 
TTCTCTCCAC CTCAGAAATA CCAACCGTTC CAAGTCAGTG GCTCTCCACA GATGATGGAC 
CAGGCGTCAA TGTACCAGTA TTCGCCACAA ACACAGAACA TGCAGCAGCC GCCGCCGCTG 
CCACCACAGC AGCAACATCA ACAGAATTAT CCCCACAACT CACCCCTGCA GTTCAGTCCT 
TACTCCAGAA TGTCCCAGTC TCCCAAGTAT GATTCCAACC TCTTTGATAC TCACGAGCCA 
CAGTTCTGCA CGGGCCAAAG TTTTGTGTCT TTACTGACTG GCCCGGGGGA ACCCGAGTCT 
CTTGCTGTTC CCGTTCCGGC TCCCACCAGC ATTCCACCCC AAACTGAGAC TCAGCTGCAG 
ACCTTCAGCC TGATGCCAAG CAATGCCTGC GAGGCCGTCG TCGGGGTGCA CGATGTGGGC 
TCGCACTCTT TGGGCACGTC CCTGTCACTC CAGAACATCA TGGGAAGCCC GATGAATACC 
ACCCAGCTGG GGAAGTCATT TTTTCAGTGG CAGGTAGAGC AGGAAGAAAG CAAATTGGCA 
AATATTCCCC AGGACCAGTT CCTCGCTAGG GACGGAGATG GTGACACGTT TCTCCATATT 
GCTGTTGCCC AAGGGAGGCG AGCGCTCTCG TATGTCCTTG CAAGAAAGAT GAACGCTCTT 
CACATGCTGG ACATTAAGGA GCACAATGGA CAGAGTGCCT TTCAGGTGGC GGTAGCTGCC 
AATCAGCACC TCATTGTGCA AGATCTGGTA AACCTTGGTG CCCAGGTGAA CACCACGGAT 
TGCTGGGGAA GGACACCTCT GCATGTCTGT GCGGAGAAGG GCCACTCCCA GGTGCTCCAG 
GCCATCCAGA AGGGAGCTGT GAGGAGCAAT CAGTTTGTGG ATCTTGAGGC AACGAACTAT 
GATGGCCTGA CTCCCCTACA TTGCGCGGTC GTGGCTCACA ATGCGGTGGT GCACGAACTG 
CAGAGGAATC GGCAGTCTCA TTCGCCCGAA GTGCAGGACC TCTTACTAAG GAATAAGAGC 
CTGGTAGACA CCATTAAGTG TCTGATTCAG ATGGGAGCCG CAGTGGAGGC AAAGGATCGT 
AAGAGTGGCC GCACAGCCCT GCACTTGGCA GCGGAAGAAG CAAATCTGGA ACTCATTCGC 
CTCTTTTTGG AGTTGCCCAG TTGCCTGTCT TTCGTGAATG CCAAGGCTTA CAATGGAAAC 
ACCGCCCTCC ATGTTGCTGC CAGCCTGCAG TATCGGGTGA CACAGTTGGA CGCAGTCCGC 
CTGTTGATGA GGAAGGGAGC TGACCCCAGT ACTCGGAACT TGGAGAATGA ACAGCCCGTG 
CATTTGGTCC CCGATGGCCC AGTGGGAGAA CAGATCCGAC GTATCCTGAA GGGGAAGTCC 
ATTCAGCAGC GAGCACCACC ATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfkbiz ( NM_001159395.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001152867.1 Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta (Nfkbiz), transcript variant 3, mRNA.

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CloneID	OMu07437
Clone ID Related Accession (Same CDS sequence)	NM_001159395.1
Accession Version	NM_001159395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	NF-kappa-B inhibitor zeta isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB047549.2 and AK159171.1. Transcript Variant: This variant (3) lacks the alternate exon that in variant 1 contains the translation start site. This results in a downstream in-frame translation start site being used. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB047549.2, AK149946.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGGGTTG GCAGGCAGCA GAGAGGCCCC TTTCAAGGTG TTCGCGTGAA GAACTCGGTG 
AAGGAGCTCC TGCTACACAT CCGAAGCAAC AAGCAGAAGG CTTCCGGCCA GCCCGTGGAT 
GAGTTTAAGA CACAGAGTGT GAACATTGAG CAGCTGACAG ACCTGAAGAG TGCAGTGTCC 
GCAGTGGGGA AAAGAAAGGG ACCCGATCCT CTGTCCGATG GACCGGTTTG CAAGAGGCCA 
GCTCTGTTGC CTTCTCACTT CGTGACATCA CCGCAAACGC CTACACCTGG GGAGAGCATG 
GAAGATGTTC GCCACAGCGA ATCCAAGCTG GATAGCAGCG CTGCCCTGCT TCAGAATATT 
ATAAACATCA AGAATGAGTG CAACCCAGTT TCACTAAACA CTGTTCAGGT TAGCTGGATG 
AGCCCCACGG TTCCCCAGAA CTCGCCAAGA GACCAGTGCC AGGATTTCCA TGGAGGCCAG 
GCCTTCTCTC CACCTCAGAA ATACCAACCG TTCCAAGTCA GTGGCTCTCC ACAGATGATG 
GACCAGGCGT CAATGTACCA GTATTCGCCA CAAACACAGA ACATGCAGCA GCCGCCGCCG 
CTGCCACCAC AGCAGCAACA TCAACAGAAT TATCCCCACA ACTCACCCCT GCAGTTCAGT 
CCTTACTCCA GAATGTCCCA GTCTCCCAAG TATGATTCCA ACCTCTTTGA TACTCACGAG 
CCACAGTTCT GCACGGGCCA AAGTTTTGTG TCTTTACTGA CTGGCCCGGG GGAACCCGAG 
TCTCTTGCTG TTCCCGTTCC GGCTCCCACC AGCATTCCAC CCCAAACTGA GACTCAGCTG 
CAGACCTTCA GCCTGATGCC AAGCAATGCC TGCGAGGCCG TCGTCGGGGT GCACGATGTG 
GGCTCGCACT CTTTGGGCAC GTCCCTGTCA CTCCAGAACA TCATGGGAAG CCCGATGAAT 
ACCACCCAGC TGGGGAAGTC ATTTTTTCAG TGGCAGGTAG AGCAGGAAGA AAGCAAATTG 
GCAAATATTC CCCAGGACCA GTTCCTCGCT AGGGACGGAG ATGGTGACAC GTTTCTCCAT 
ATTGCTGTTG CCCAAGGGAG GCGAGCGCTC TCGTATGTCC TTGCAAGAAA GATGAACGCT 
CTTCACATGC TGGACATTAA GGAGCACAAT GGACAGAGTG CCTTTCAGGT GGCGGTAGCT 
GCCAATCAGC ACCTCATTGT GCAAGATCTG GTAAACCTTG GTGCCCAGGT GAACACCACG 
GATTGCTGGG GAAGGACACC TCTGCATGTC TGTGCGGAGA AGGGCCACTC CCAGGTGCTC 
CAGGCCATCC AGAAGGGAGC TGTGAGGAGC AATCAGTTTG TGGATCTTGA GGCAACGAAC 
TATGATGGCC TGACTCCCCT ACATTGCGCG GTCGTGGCTC ACAATGCGGT GGTGCACGAA 
CTGCAGAGGA ATCGGCAGTC TCATTCGCCC GAAGTGCAGG ACCTCTTACT AAGGAATAAG 
AGCCTGGTAG ACACCATTAA GTGTCTGATT CAGATGGGAG CCGCAGTGGA GGCAAAGGAT 
CGTAAGAGTG GCCGCACAGC CCTGCACTTG GCAGCGGAAG AAGCAAATCT GGAACTCATT 
CGCCTCTTTT TGGAGTTGCC CAGTTGCCTG TCTTTCGTGA ATGCCAAGGC TTACAATGGA 
AACACCGCCC TCCATGTTGC TGCCAGCCTG CAGTATCGGG TGACACAGTT GGACGCAGTC 
CGCCTGTTGA TGAGGAAGGG AGCTGACCCC AGTACTCGGA ACTTGGAGAA TGAACAGCCC 
GTGCATTTGG TCCCCGATGG CCCAGTGGGA GAACAGATCC GACGTATCCT GAAGGGGAAG 
TCCATTCAGC AGCGAGCACC ACCATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001152867.1
CDS	265..2154
Translation	MGVGRQQRGPFQGVRVKNSVKELLLHIRSNKQKASGQPVDEFKTQSVNIEQLTDLKSAVSAVGKRKGPDPLSDGPVCKRPALLPSHFVTSPQTPTPGESMEDVRHSESKLDSSAALLQNIINIKNECNPVSLNTVQVSWMSPTVPQNSPRDQCQDFHGGQAFSPPQKYQPFQVSGSPQMMDQASMYQYSPQTQNMQQPPPLPPQQQHQQNYPHNSPLQFSPYSRMSQSPKYDSNLFDTHEPQFCTGQSFVSLLTGPGEPESLAVPVPAPTSIPPQTETQLQTFSLMPSNACEAVVGVHDVGSHSLGTSLSLQNIMGSPMNTTQLGKSFFQWQVEQEESKLANIPQDQFLARDGDGDTFLHIAVAQGRRALSYVLARKMNALHMLDIKEHNGQSAFQVAVAANQHLIVQDLVNLGAQVNTTDCWGRTPLHVCAEKGHSQVLQAIQKGAVRSNQFVDLEATNYDGLTPLHCAVVAHNAVVHELQRNRQSHSPEVQDLLLRNKSLVDTIKCLIQMGAAVEAKDRKSGRTALHLAAEEANLELIRLFLELPSCLSFVNAKAYNGNTALHVAASLQYRVTQLDAVRLLMRKGADPSTRNLENEQPVHLVPDGPVGEQIRRILKGKSIQQRAPPY

Target ORF information:

RefSeq Version	NM_001159395.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta (Nfkbiz), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159395.1

ORF Insert Sequence:

ATGGGGGTTG GCAGGCAGCA GAGAGGCCCC TTTCAAGGTG TTCGCGTGAA GAACTCGGTG 
AAGGAGCTCC TGCTACACAT CCGAAGCAAC AAGCAGAAGG CTTCCGGCCA GCCCGTGGAT 
GAGTTTAAGA CACAGAGTGT GAACATTGAG CAGCTGACAG ACCTGAAGAG TGCAGTGTCC 
GCAGTGGGGA AAAGAAAGGG ACCCGATCCT CTGTCCGATG GACCGGTTTG CAAGAGGCCA 
GCTCTGTTGC CTTCTCACTT CGTGACATCA CCGCAAACGC CTACACCTGG GGAGAGCATG 
GAAGATGTTC GCCACAGCGA ATCCAAGCTG GATAGCAGCG CTGCCCTGCT TCAGAATATT 
ATAAACATCA AGAATGAGTG CAACCCAGTT TCACTAAACA CTGTTCAGGT TAGCTGGATG 
AGCCCCACGG TTCCCCAGAA CTCGCCAAGA GACCAGTGCC AGGATTTCCA TGGAGGCCAG 
GCCTTCTCTC CACCTCAGAA ATACCAACCG TTCCAAGTCA GTGGCTCTCC ACAGATGATG 
GACCAGGCGT CAATGTACCA GTATTCGCCA CAAACACAGA ACATGCAGCA GCCGCCGCCG 
CTGCCACCAC AGCAGCAACA TCAACAGAAT TATCCCCACA ACTCACCCCT GCAGTTCAGT 
CCTTACTCCA GAATGTCCCA GTCTCCCAAG TATGATTCCA ACCTCTTTGA TACTCACGAG 
CCACAGTTCT GCACGGGCCA AAGTTTTGTG TCTTTACTGA CTGGCCCGGG GGAACCCGAG 
TCTCTTGCTG TTCCCGTTCC GGCTCCCACC AGCATTCCAC CCCAAACTGA GACTCAGCTG 
CAGACCTTCA GCCTGATGCC AAGCAATGCC TGCGAGGCCG TCGTCGGGGT GCACGATGTG 
GGCTCGCACT CTTTGGGCAC GTCCCTGTCA CTCCAGAACA TCATGGGAAG CCCGATGAAT 
ACCACCCAGC TGGGGAAGTC ATTTTTTCAG TGGCAGGTAG AGCAGGAAGA AAGCAAATTG 
GCAAATATTC CCCAGGACCA GTTCCTCGCT AGGGACGGAG ATGGTGACAC GTTTCTCCAT 
ATTGCTGTTG CCCAAGGGAG GCGAGCGCTC TCGTATGTCC TTGCAAGAAA GATGAACGCT 
CTTCACATGC TGGACATTAA GGAGCACAAT GGACAGAGTG CCTTTCAGGT GGCGGTAGCT 
GCCAATCAGC ACCTCATTGT GCAAGATCTG GTAAACCTTG GTGCCCAGGT GAACACCACG 
GATTGCTGGG GAAGGACACC TCTGCATGTC TGTGCGGAGA AGGGCCACTC CCAGGTGCTC 
CAGGCCATCC AGAAGGGAGC TGTGAGGAGC AATCAGTTTG TGGATCTTGA GGCAACGAAC 
TATGATGGCC TGACTCCCCT ACATTGCGCG GTCGTGGCTC ACAATGCGGT GGTGCACGAA 
CTGCAGAGGA ATCGGCAGTC TCATTCGCCC GAAGTGCAGG ACCTCTTACT AAGGAATAAG 
AGCCTGGTAG ACACCATTAA GTGTCTGATT CAGATGGGAG CCGCAGTGGA GGCAAAGGAT 
CGTAAGAGTG GCCGCACAGC CCTGCACTTG GCAGCGGAAG AAGCAAATCT GGAACTCATT 
CGCCTCTTTT TGGAGTTGCC CAGTTGCCTG TCTTTCGTGA ATGCCAAGGC TTACAATGGA 
AACACCGCCC TCCATGTTGC TGCCAGCCTG CAGTATCGGG TGACACAGTT GGACGCAGTC 
CGCCTGTTGA TGAGGAAGGG AGCTGACCCC AGTACTCGGA ACTTGGAGAA TGAACAGCCC 
GTGCATTTGG TCCCCGATGG CCCAGTGGGA GAACAGATCC GACGTATCCT GAAGGGGAAG 
TCCATTCAGC AGCGAGCACC ACCATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfkbiz ( XM_030249339.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105199.1 Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta (Nfkbiz), transcript variant X1, mRNA.

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CloneID	OMu07434
Clone ID Related Accession (Same CDS sequence)	XM_030249339.1 , NM_001159394.1 , NM_030612.3
Accession Version	XM_030249339.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NF-kappa-B inhibitor zeta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGTGG ACAAGCTGCT GGACGACAGC CGCGGCGGAG AGGGGCTGCT GGACGCGGCC 
GGCGACTGCG GCCTCATGAC CAGCCCGCTC AACCTGGCTT ACTTCTACGG CGCCTCGCCT 
CCTTCCGCCC CCGGCGCCGG CGACACCGGC TACCTGTCCG CCGTGCCCTC CGCGCCCGGC 
TCGCCCGGCT CCGACTCCTC CGATTTCTCC TCCACTTCCT CCGTGTCCTC CTGCGGGGCC 
GTGGAGTCCC GTCCCAGAGG TGGCGCCCGC GCCGAGCGCC CTCAAGTTGA GCCCCACATG 
GGGGTTGGCA GGCAGCAGAG AGGCCCCTTT CAAGGTGTTC GCGTGAAGAA CTCGGTGAAG 
GAGCTCCTGC TACACATCCG AAGCAACAAG CAGAAGGCTT CCGGCCAGCC CGTGGATGAG 
TTTAAGACAC AGAGTGTGAA CATTGAGCAG CTGACAGACC TGAAGAGTGC AGTGTCCGCA 
GTGGGGAAAA GAAAGGGACC CGATCCTCTG TCCGATGGAC CGGTTTGCAA GAGGCCAGCT 
CTGTTGCCTT CTCACTTCGT GACATCACCG CAAACGCCTA CACCTGGGGA GAGCATGGAA 
GATGTTCGCC ACAGCGAATC CAAGCTGGAT AGCAGCGCTG CCCTGCTTCA GAATATTATA 
AACATCAAGA ATGAGTGCAA CCCAGTTTCA CTAAACACTG TTCAGGTTAG CTGGATGAGC 
CCCACGGTTC CCCAGAACTC GCCAAGAGAC CAGTGCCAGG ATTTCCATGG AGGCCAGGCC 
TTCTCTCCAC CTCAGAAATA CCAACCGTTC CAAGTCAGTG GCTCTCCACA GATGATGGAC 
CAGGCGTCAA TGTACCAGTA TTCGCCACAA ACACAGAACA TGCAGCAGCC GCCGCCGCTG 
CCACCACAGC AGCAACATCA ACAGAATTAT CCCCACAACT CACCCCTGCA GTTCAGTCCT 
TACTCCAGAA TGTCCCAGTC TCCCAAGTAT GATTCCAACC TCTTTGATAC TCACGAGCCA 
CAGTTCTGCA CGGGCCAAAG TTTTGTGTCT TTACTGACTG GCCCGGGGGA ACCCGAGTCT 
CTTGCTGTTC CCGTTCCGGC TCCCACCAGC ATTCCACCCC AAACTGAGAC TCAGCTGCAG 
ACCTTCAGCC TGATGCCAAG CAATGCCTGC GAGGCCGTCG TCGGGGTGCA CGATGTGGGC 
TCGCACTCTT TGGGCACGTC CCTGTCACTC CAGAACATCA TGGGAAGCCC GATGAATACC 
ACCCAGCTGG GGAAGTCATT TTTTCAGTGG CAGGTAGAGC AGGAAGAAAG CAAATTGGCA 
AATATTCCCC AGGACCAGTT CCTCGCTAGG GACGGAGATG GTGACACGTT TCTCCATATT 
GCTGTTGCCC AAGGGAGGCG AGCGCTCTCG TATGTCCTTG CAAGAAAGAT GAACGCTCTT 
CACATGCTGG ACATTAAGGA GCACAATGGA CAGAGTGCCT TTCAGGTGGC GGTAGCTGCC 
AATCAGCACC TCATTGTGCA AGATCTGGTA AACCTTGGTG CCCAGGTGAA CACCACGGAT 
TGCTGGGGAA GGACACCTCT GCATGTCTGT GCGGAGAAGG GCCACTCCCA GGTGCTCCAG 
GCCATCCAGA AGGGAGCTGT GAGGAGCAAT CAGTTTGTGG ATCTTGAGGC AACGAACTAT 
GATGGCCTGA CTCCCCTACA TTGCGCGGTC GTGGCTCACA ATGCGGTGGT GCACGAACTG 
CAGAGGAATC GGCAGTCTCA TTCGCCCGAA GTGCAGGACC TCTTACTAAG GAATAAGAGC 
CTGGTAGACA CCATTAAGTG TCTGATTCAG ATGGGAGCCG CAGTGGAGGC AAAGGATCGT 
AAGAGTGGCC GCACAGCCCT GCACTTGGCA GCGGAAGAAG CAAATCTGGA ACTCATTCGC 
CTCTTTTTGG AGTTGCCCAG TTGCCTGTCT TTCGTGAATG CCAAGGCTTA CAATGGAAAC 
ACCGCCCTCC ATGTTGCTGC CAGCCTGCAG TATCGGGTGA CACAGTTGGA CGCAGTCCGC 
CTGTTGATGA GGAAGGGAGC TGACCCCAGT ACTCGGAACT TGGAGAATGA ACAGCCCGTG 
CATTTGGTCC CCGATGGCCC AGTGGGAGAA CAGATCCGAC GTATCCTGAA GGGGAAGTCC 
ATTCAGCAGC GAGCACCACC ATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105199.1
CDS	4059..6245
Translation	MIVDKLLDDSRGGEGLLDAAGDCGLMTSPLNLAYFYGASPPSAPGAGDTGYLSAVPSAPGSPGSDSSDFSSTSSVSSCGAVESRPRGGARAERPQVEPHMGVGRQQRGPFQGVRVKNSVKELLLHIRSNKQKASGQPVDEFKTQSVNIEQLTDLKSAVSAVGKRKGPDPLSDGPVCKRPALLPSHFVTSPQTPTPGESMEDVRHSESKLDSSAALLQNIINIKNECNPVSLNTVQVSWMSPTVPQNSPRDQCQDFHGGQAFSPPQKYQPFQVSGSPQMMDQASMYQYSPQTQNMQQPPPLPPQQQHQQNYPHNSPLQFSPYSRMSQSPKYDSNLFDTHEPQFCTGQSFVSLLTGPGEPESLAVPVPAPTSIPPQTETQLQTFSLMPSNACEAVVGVHDVGSHSLGTSLSLQNIMGSPMNTTQLGKSFFQWQVEQEESKLANIPQDQFLARDGDGDTFLHIAVAQGRRALSYVLARKMNALHMLDIKEHNGQSAFQVAVAANQHLIVQDLVNLGAQVNTTDCWGRTPLHVCAEKGHSQVLQAIQKGAVRSNQFVDLEATNYDGLTPLHCAVVAHNAVVHELQRNRQSHSPEVQDLLLRNKSLVDTIKCLIQMGAAVEAKDRKSGRTALHLAAEEANLELIRLFLELPSCLSFVNAKAYNGNTALHVAASLQYRVTQLDAVRLLMRKGADPSTRNLENEQPVHLVPDGPVGEQIRRILKGKSIQQRAPPY

Target ORF information:

RefSeq Version	XM_030249339.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta (Nfkbiz), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249339.1

ORF Insert Sequence:

ATGATCGTGG ACAAGCTGCT GGACGACAGC CGCGGCGGAG AGGGGCTGCT GGACGCGGCC 
GGCGACTGCG GCCTCATGAC CAGCCCGCTC AACCTGGCTT ACTTCTACGG CGCCTCGCCT 
CCTTCCGCCC CCGGCGCCGG CGACACCGGC TACCTGTCCG CCGTGCCCTC CGCGCCCGGC 
TCGCCCGGCT CCGACTCCTC CGATTTCTCC TCCACTTCCT CCGTGTCCTC CTGCGGGGCC 
GTGGAGTCCC GTCCCAGAGG TGGCGCCCGC GCCGAGCGCC CTCAAGTTGA GCCCCACATG 
GGGGTTGGCA GGCAGCAGAG AGGCCCCTTT CAAGGTGTTC GCGTGAAGAA CTCGGTGAAG 
GAGCTCCTGC TACACATCCG AAGCAACAAG CAGAAGGCTT CCGGCCAGCC CGTGGATGAG 
TTTAAGACAC AGAGTGTGAA CATTGAGCAG CTGACAGACC TGAAGAGTGC AGTGTCCGCA 
GTGGGGAAAA GAAAGGGACC CGATCCTCTG TCCGATGGAC CGGTTTGCAA GAGGCCAGCT 
CTGTTGCCTT CTCACTTCGT GACATCACCG CAAACGCCTA CACCTGGGGA GAGCATGGAA 
GATGTTCGCC ACAGCGAATC CAAGCTGGAT AGCAGCGCTG CCCTGCTTCA GAATATTATA 
AACATCAAGA ATGAGTGCAA CCCAGTTTCA CTAAACACTG TTCAGGTTAG CTGGATGAGC 
CCCACGGTTC CCCAGAACTC GCCAAGAGAC CAGTGCCAGG ATTTCCATGG AGGCCAGGCC 
TTCTCTCCAC CTCAGAAATA CCAACCGTTC CAAGTCAGTG GCTCTCCACA GATGATGGAC 
CAGGCGTCAA TGTACCAGTA TTCGCCACAA ACACAGAACA TGCAGCAGCC GCCGCCGCTG 
CCACCACAGC AGCAACATCA ACAGAATTAT CCCCACAACT CACCCCTGCA GTTCAGTCCT 
TACTCCAGAA TGTCCCAGTC TCCCAAGTAT GATTCCAACC TCTTTGATAC TCACGAGCCA 
CAGTTCTGCA CGGGCCAAAG TTTTGTGTCT TTACTGACTG GCCCGGGGGA ACCCGAGTCT 
CTTGCTGTTC CCGTTCCGGC TCCCACCAGC ATTCCACCCC AAACTGAGAC TCAGCTGCAG 
ACCTTCAGCC TGATGCCAAG CAATGCCTGC GAGGCCGTCG TCGGGGTGCA CGATGTGGGC 
TCGCACTCTT TGGGCACGTC CCTGTCACTC CAGAACATCA TGGGAAGCCC GATGAATACC 
ACCCAGCTGG GGAAGTCATT TTTTCAGTGG CAGGTAGAGC AGGAAGAAAG CAAATTGGCA 
AATATTCCCC AGGACCAGTT CCTCGCTAGG GACGGAGATG GTGACACGTT TCTCCATATT 
GCTGTTGCCC AAGGGAGGCG AGCGCTCTCG TATGTCCTTG CAAGAAAGAT GAACGCTCTT 
CACATGCTGG ACATTAAGGA GCACAATGGA CAGAGTGCCT TTCAGGTGGC GGTAGCTGCC 
AATCAGCACC TCATTGTGCA AGATCTGGTA AACCTTGGTG CCCAGGTGAA CACCACGGAT 
TGCTGGGGAA GGACACCTCT GCATGTCTGT GCGGAGAAGG GCCACTCCCA GGTGCTCCAG 
GCCATCCAGA AGGGAGCTGT GAGGAGCAAT CAGTTTGTGG ATCTTGAGGC AACGAACTAT 
GATGGCCTGA CTCCCCTACA TTGCGCGGTC GTGGCTCACA ATGCGGTGGT GCACGAACTG 
CAGAGGAATC GGCAGTCTCA TTCGCCCGAA GTGCAGGACC TCTTACTAAG GAATAAGAGC 
CTGGTAGACA CCATTAAGTG TCTGATTCAG ATGGGAGCCG CAGTGGAGGC AAAGGATCGT 
AAGAGTGGCC GCACAGCCCT GCACTTGGCA GCGGAAGAAG CAAATCTGGA ACTCATTCGC 
CTCTTTTTGG AGTTGCCCAG TTGCCTGTCT TTCGTGAATG CCAAGGCTTA CAATGGAAAC 
ACCGCCCTCC ATGTTGCTGC CAGCCTGCAG TATCGGGTGA CACAGTTGGA CGCAGTCCGC 
CTGTTGATGA GGAAGGGAGC TGACCCCAGT ACTCGGAACT TGGAGAATGA ACAGCCCGTG 
CATTTGGTCC CCGATGGCCC AGTGGGAGAA CAGATCCGAC GTATCCTGAA GGGGAAGTCC 
ATTCAGCAGC GAGCACCACC ATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The resident pathobiont Staphylococcus xylosus in Nfkbiz-deficient skin accelerates spontaneous skin inflammation.
Scientific reports7(1)6348(2017 Jul)
Kim Y,Lee YS,Yang JY,Lee SH,Park YY,Kweon MN

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Nfkbiz cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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