Sptbn4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sptbn4
Entrez Gene ID	80297
Full Name	spectrin beta, non-erythrocytic 4
Synonyms	1700022P15Rik,5830426A08Rik,ROSA62,SpbIV,Spnb4,dyn,lnd,nmf261,nmf379,qv
General protein information	Preferred Names spectrin beta, non-erythrocytic 4 Names spectrin beta chain, non-erythrocytic 4 beta-spectrin 4 lumbosacral neuroaxonal dystrophy neuroaxonal dystrophy quivering spectrin beta 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 A3\|7 15.88 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001199234.1	NP_001186163.1	spectrin beta chain, non-erythrocytic 4 isoform sigma6
NM_001199235.1	NP_001186164.1	spectrin beta chain, non-erythrocytic 4 isoform sigma6
XM_006540437.2	XP_006540500.1	spectrin beta chain, non-erythrocytic 4 isoform X2
NM_032610.2	NP_115999.2	spectrin beta chain, non-erythrocytic 4 isoform sigma1
NM_001199236.1	NP_001186165.1	spectrin beta chain, non-erythrocytic 4 isoform sigma6
XM_006540436.3	XP_006540499.1	spectrin beta chain, non-erythrocytic 4 isoform X1
XM_006540436.2	XP_006540499.1	spectrin beta chain, non-erythrocytic 4 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sptbn4	NP_001094315.1
	SPTBN4	XP_002801295.1
Canis lupus familiaris (dog)	SPTBN4	XP_541613.2
Bos taurus (cattle)	SPTBN4	NP_001179839.1
Xenopus tropicalis (tropical clawed frog)	sptbn4	XP_002938861.2
Homo sapiens (human)	SPTBN4	NP_066022.2
Mus musculus (house mouse)	Sptbn4	NP_115999.2

Gene Ontology
Bibliography

Related articles in PubMed

Schwannomin-interacting Protein 1 Isoform IQCJ-SCHIP1 Is a Multipartner Ankyrin- and Spectrin-binding Protein Involved in the Organization of Nodes of Ranvier.
Martin PM, Cifuentes-Diaz C, Devaux J, Garcia M, Bureau J, Thomasseau S, Klingler E, Girault JA, Goutebroze L
The Journal of biological chemistry292(6)2441-2456(2017 02)

Postnatal Development of Spasticity Following Transgene Insertion in the Mouse ?IV Spectrin Gene (SPTBN4).
Kichkin E, Visvanathan A, Lovicu FJ, Shu DY, Das SJ, Reddel SW, McCann EP, Zhang KY, Williams KL, Blair IP, Phillips WD
Journal of neuromuscular diseases4(2)159-164(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In embryonic mouse axons neither beta4-spectrin nor Neurofascin control the distal-to-proximal restriction of AnkG.
Title: In vivo assembly of the axon initial segment in motor neurons.

provide new insight into membrane targeting of TREK-1 in the heart and establish a broader role for beta(IV)-spectrin in organizing functional membrane domains critical for normal heart function
Title: ?(IV)-Spectrin regulates TREK-1 membrane targeting in the heart.

Data indicate that betaIV-Spectrin-targeted CaMKII directly phosphorylates the inwardly-rectifying potassium channel, Kir6.2.
Title: ?IV-Spectrin and CaMKII facilitate Kir6.2 regulation in pancreatic beta cells.

These findings demonstrate that spectrin cytoskeleton finely regulates ion channel distribution and implicates KCNQ2/3 subunits in axonal excitability and in myokymia etiology.
Title: The C-terminal domain of ?IV-spectrin is crucial for KCNQ2 aggregation and excitability at nodes of Ranvier.

identified a regulatory mechanism for Na(+) channels, via direct phosphorylation by beta(IV)-spectrin-targeted calcium/calmodulin-dependent kinase II
Title: A ?(IV)-spectrin/CaMKII signaling complex is essential for membrane excitability in mice.

The following Sptbn4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sptbn4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu38545	XM_006540436.3 Latest version!	Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu02126	NM_001199235.1 Latest version!	Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant sigma6b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu02126	NM_001199234.1 Latest version!	Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant sigma6a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu02126	NM_001199236.1 Latest version!	Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant sigma6c, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu20714	NM_032610.2 Latest version!	Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant sigma1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OMu38546	XM_006540437.2 Latest version!	Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
View Old Accession Versions >>
OMu38545	XM_006540436.2	Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sptbn4 ( XM_006540436.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540499.1 Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu38545
Clone ID Related Accession (Same CDS sequence)	XM_006540436.3 , XM_006540436.2
Accession Version	XM_006540436.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540436.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGATCT GGCCGAGCGG CGGGCCGGTG GGGCCGAACA GAGCCGGGCC CGGCCCGGCG 
ACGAACGCCC GCAGGGAGGG CGGTGCTGCG CGGCGAGCAT CCGGGACCGC ATCGGCGGCC 
GGCGCCCAGG CCCTGCCTCC CCCAATGGCA CAAGTACCAG GGGAGGTGGA CAACATGGAG 
GGCCCAGCAG TCTCCAACAA CAACAACCCT TCAGCTCGCT GGGAGAGCCC AGATCGGGGC 
TGGGATCGGG AACCTCCCGC AGCCGCCAAT GCTGCGGCTT CACTCTTTGA GTGCTCTCGG 
ATCAAGGCTT TAGCAGATGA GCGAGAGGCT GTGCAGAAGA AAACCTTCAC CAAGTGGGTG 
AATTCGCACC TTGCCCGCGT GGGCTGCCAC ATTGGAGACC TGTATGCAGA CCTTCGGGAC 
GGGTTTGTCC TCACCCGGCT TCTGGAAGTG CTGTCGGGGG AGCAGCTGCC GAGGCCCACT 
CGCGGCCGCA TGCGGATCCA CTCGCTGGAG AACGTGGACA AGGCTCTGCA GTTCCTGAAG 
GAGCAGCGTG TGCATCTGGA GAACGTGGGG TCACATGACA TCGTGGATGG GAACCACCGA 
CTGACGCTGG GGCTGGTCTG GACCATCATC CTGCGCTTCC AGATTCAAGT CATCAAGATT 
GAGACAGAAG ACAACAGAGA GACCCGCTCC GCCAAGGACG CTCTGCTCTT GTGGTGCCAG 
ATGAAAACAG CCGGTTACCC TGAGGTAAAC ATCCAGAATT TCACCACCAG CTGGAGAGAT 
GGCCTGGCCT TCAATGCCCT CATTCACCGT CACAGGCCTG ATCTTGTGGA CCTCAGCAAA 
CTTACCAAGT CCAACGCCAA CTACAACCTG CAGAGAGCTT TCCGCACAGC CGAGCAGCAC 
CTGGGGCTAG CACGTCTGCT GGACCCCGAA GATGTGAACA TGGAGGCTCC AGATGAGAAG 
TCCATTATCA CCTATGTGGT CTCCTTCTAC CACTACTTCT CCAAGATGAA GGCTCTGGCG 
GTGGAGGGAA AACGGATTGG AAAGGTCCTG GACCAGGTGT TGGAGGTGGA CAAGATCATA 
GAACGCTATG AGGAGCTGGC GGCGGAGCTG TTGGCCTGGA TCCATCGCAC TGTGGACCTC 
ATTAGCAACC AAAAGTTTGC TAACTCACTG AGTGGGGTGC AGCAGCAGCT GCAGGCCTTC 
ACGGCTTACT GTACGCTGGA GAAGCCCGTC AAGTTTCAGG AGAAGGGGAA CCTGGAGGTG 
CTACTCTTCA GCATCCAAAG CAAACTGCGC GCCCACAACC GGCGCCTCTT TGTGCCTCGA 
GAAGGCTGTG GCATCTGGGA TATTGACAAG GCGTGGGGTG AGCTGGAGAA GGCAGAGCAT 
GAGCGAGAGG CGGCCCTCAG AGCTGAGCTC ATCCGGCAGG AGAAGTTGGA ACTCTTGGCT 
CAAAGATTTG ACCACAAGGT GGCCATGAGG GAGAGCTGGC TAAATGAGAA CCAGCGCCTG 
GTCTCCCAGG ACAACTTCGG ATATGAGCTG CCGGCAGTGG AGGCAGCCAT GAAGAAGCAC 
GAAGCCATTG AGGCAGACAT TGCAGCCTAC GAGGAGCGGG TTCAGGGTGT GGCGGAGTTA 
GCCCAGGCGT TGGCAGCTGA AGGCTACTAT GATGCCCGGC GGGTGGCTGC CCAGCGTGAC 
AGTGTCCTTC GCCAGTGGGC CCTGCTGACC GGGCTGGTAG GTGCCCGGCG GACCCGGCTT 
GAGCAGAATC TGGCCCTGCA GAAGGTCTTC CAGGAGATGG TCTACATGGT GGACTGGATG 
GAGGAGATGC AGACTCAGCT GCTGTCTCGG GAATGTGGGC AGCACCTGGT GGAGGCCGAT 
GACCTGCTGC AGAAGCACGG GCTGCTGGAG GGGGACATCG CAGCCCAGAG TGAGCGAGTG 
GAGGCCCTCA ATGCTGCAGC CCTGAGATTC TCCCAGCTAC AGGGCTATCA GCCCTGCGAC 
CCTCAGGTCA TTTGCAACCG TGTGAACCAC GTGCATGGCT GTCTGTCGGA GCTGCAGGAG 
CAGGCAGCAC GGCGGCGCGC AGAGCTGGAA GCTTCTCGGA GCCTGTGGGC GCTGCTGCAG 
GAGCTGGAGG AGGCGGAGAG TTGGGCGCGG GACAAGGAGC GCCTCCTAGA GGCCACGAGC 
GGCAGTGGTG GCGCGGCGGG CACGGCGGGC GGCGCGCACG ACCTGTCCAG CACCGCTCGC 
CTGCTGGCGC AGCATAAGAT CCTGCAGGGC GAGCTGGGCG GCCGGCGGGC GCTGCTGCAG 
CAGGCGCTGC GACGCGGGGA GGAGTTGGCT GCGGCGGGCG GCTCCGTGGG CCCGGGCGCC 
GAGCCTTTGC ATTTGGCTGG CCTGGCGGAG CGCGCGGCTA GCGCTCGGCG CCGCTGGCAG 
CGGCTGGAAG AGGCGGCTGC GCGCCGGGAA CGGCGGCTGC AGGAGGCGCG GGCGCTGCAC 
CAATTTGGCG CCGACCTGGA CGGGCTGCTG GACTGGCTTC GTGACGCGTA TCGTCTAGCA 
GCCGCTGGAG ATTTTGGTCA CGACGAAGCG TCCAGCCGTC GCCTGGCGCG CCAGCACCGC 
GCTCTCACCG GGGAGGTGGA AGCGCACCGT GGCCCTGTGG GTGGCCTGAG GCGCCAGCTG 
GCGACACTGG GAGGAGCCAG TGGCGCAGGG CCGCTGGTGG TGGCTCTGCA GGTGCGCGTG 
GTGGAAGCCG AACAGTTGTT CGCCGAGGTG ACCGAGGTGG CCGCGCTGCG ACGACAGTGG 
CTTCGGGACG CGCTCGCCGT GTACCGCATG TTCGGCGAGG TACATGCCTG CGAGTTGTGG 
ATCGGGGAGA AAGAGCAGTG GCTGCTGGCC ATGCGTGTGC CCGATTCCCT CGACGACGTT 
GAGGTGGTGC AGCATCGATT TGAAAGTCTT GACCAGGAAA TGAACAGCCT GATGGGCCGA 
GTCCTGGATG TGAACCAAAC AGTTCAGGAG TTGGTAGAAG GAGGTCATCC CAGCTCCGAT 
GAGGTGCGCT CCTGTCAGGA CCATCTCAAC AGCAGGTGGA ACCGCATCGT GGAGTTGGTG 
GAACAGCGCA AAGAGGAGAT GAGCGCGGTG CTGCTGGTGG AGAACCACGT GCTGGAGGTG 
GCCGAGGTGC GCGCCCAAGT GCGCGAGAAG CGACGGGCAG TGGAGAGCGC GCCCCGGGCG 
GGGGGAGCCC TGCAATGGCG CCTGAGTGGC CTGGAGGCGG CTCTACAGGC GCTAGAGCCG 
CGACAGGCGG CGCTGCTGGA GGAGGCGGCG CTGCTGGCCG AACGCTTCCC CGCACAGGCG 
ACGCGCCTGC ACCAGGGCGC CGAGGAGCTA GGAGCCGAGT GGGGCGCACT GGCCGGAGCG 
GCTCAGGCCT GCGGCGAGGC GGTTGCGGCG GCAGGTCGCC TGCAGCGTTT CCTGCGCGAT 
TTGGATACTT TCCTGGACTG GCTGGTGCGG GCGCAGGAGG CAGCAGGCGC CGTCGAGGGG 
CCGCTGCCTC GCAGCCTGGA GGAGGCGGAT GGGCTGCTGG CGCGCCACGC CGCGCTCAAG 
GAGGAGGTGG ATCAGCGTGA GGAGGACTAC GCGCGCATCG TGGCTGCCAG TGAAGCGCTG 
CTAGCGTCCG AGGGCGCGGA GCTGGGCCCC GGTCTGGCGC TAGACGAGTG GCTGCCGCAC 
CTCGAGGTTG GCTGGCACAA ACTGCTCGGC TTGTGGGAGG AGCGCAGGGA GGCGCTGGTC 
CAAGCGCACG TCTATCAACT CTTCCTGAGA GACCTGTGCC AGGCGCTTGC GGTGCTGCGC 
AACCAGGAGG TGGCGCTGTC TGGCGCTGAA CTCCCTTGCA CAGTGGAGTC GGTAGAAGAG 
GCGATGAAAA GGCACCGGGA TTTTCTCACC ACGATGGAGC TGAACCAGCA AAAGATGCAG 
GTGGCGGTTC AAGCTGCAGA GAGCCTGCTG CGGCAAGGCA ACGCCTACGG GGAGCAGGCG 
CAGGAGGCAG TGGCTCGGCT GCTAGAGAAG AGCCAAGAAA ATCAGCTGAG GGCCCAGCAG 
TGGATGCAAA AACTACTGGA CCAGCTTGTG CTGCAGCACT TTCTTAGAGA TTGTCATGAG 
CTGGATGGCT GGATCCATGA GAAGATGCTG ATGGCTCGTG ATGGCACCCG GGAAGACAGC 
CACAAGCTGC ACAAGAGATG GCTCCGGCAC CAGGCGTTCA TGGCTGAGCT GGCTCAGAAT 
AAGGAGTGGC TGGAGAAGAT AGAAAGGGAG GGCCAGCAAC TGATGCAGGA GAAACCGGAG 
CTGGCAGCCT CTGTGCGCAA GAAATTAGGT GAGATCCGGC AGTGCTGGGC GGAGCTGGAG 
AGCACCACAC AGGCCAAGGC GCGCCAGCTC TTCGAGGCCA GCAAGGCGGA CCAGCTAGTG 
CAGAGCTTCG CGGAGCTGGA CAAGCGGCTG CTTCACATGG AAAGCCAGCT GCAAGACGTA 
GACCCCGGTG GGGACCTGGC CACTGTCAAC AGCCAGCTCA AGAAGTTACA GTCCATGGAG 
TCTCAGGTGG AGGAGTGGTG CCGAGAGGTG GGCGAGCTTC AGGCGCAGAC GGCGGCGCTT 
CCGCTGGAGC AGGCGAGCAA GGAGTTGGTG GGGGAGCGGC AGAGTGCGGT GGGCGAGCGC 
CTGGTGCGCC TGCTGGAGCC TTTGCAGGAG CGACGCCGCT TGTTGCTGGC ATCCAAGGAG 
CTGCATCAGG TGGCCCACGA CCTGGACGAT GAACTGGCTT GGGTTCAAGA GCGGCTGCCA 
CTGGCCATGC AGACAGAGCG AGGCACTGGC TTGCAGGCTG TCCAGCAGCA CATCAAAAAG 
AACCAGGGCC TGCGGCGGGA GATCCAGGCT CACGGGCCGC GCTTGGAGGA GGTGCTGGAG 
CGCGCGGGCG TGCTGGCCTC GCTGCGCAGC CCAGAGGCCG AAGCGGTGCG CCGTGGCCAA 
GAGCAGCTGC AGAGCGCCTG GACCGGGCTG CGGGAAGCGG CCGAACGGCG GCAGCAGACG 
CTGGACGCCG CCTTCCAGGT GGAACAGTAC TACTTTGACG TGGCTGAGGT GGAGGCATGG 
CTGGGCGAGC AGGAGCTGCT CATGATGAGT GAGGACAAGG GCAAGGATGA ACAGAGCACC 
CTGCAGCTGC TCAAGAAGCA TTTACAACTG GAACAGGGCG TGGAGAACTA TGAGGAAAGC 
ATCGCCCAGC TGTCACGCCA GTGCCGGGCG CTACTGGAAA TGGGACACCC AGACAGTGAG 
CAAATCAGCC GCCGACAGTC CCAAGTGGAT CGCCTCTATG TGGCGCTCAA GGAGCTGGGC 
GAGGAACGCA GGGTGAGCCT GGAACAGCAG TACTGGCTCT ACCAGCTCAG CCGCCAGGTG 
GATGAGCTGG AACACTGGAT AGCCGAGAAG GAGGTGGTAG CTGGCTCCCC AGAGCTGGGC 
CAGGACTTCG AACACGTGTC GGTGCTACAG GAGAAATTCT CAGAGTTTGC CAGTGAGACA 
GGAACCGCAG GGCGGGAGCG GCTGGCGGCG GTCAACCAGA TGGTGGACGA GCTGATTGAG 
TGTGGTCACA CAGCAGCGGC CACCATGGCT GAGTGGAAGG ACGGGCTGAA CGAGGCCTGG 
GCTGAGCTGC TGGAACTCAT GGGCACTCGG GCCCAGCTGC TCGCTGCCTC TCGGGAGCTG 
CATAAGTTCT TCAGCGATGC CCGGGAGCTT CAAGGGCAGA TTGAGGAGAA GCGGAGGAGG 
CTGCCCCGCC TGACGGCACC ACCTGAACCC AGACCCAGTG CCAGCTCCAT GCAGCGGACC 
CTGCGAGCCT TTGAGCACGA CCTGCAGCTC CTCGTGTCCC AGGTACGGCA GCTGCAGGAA 
GGGGCGGCCC AGCTGCGGAC GGTGTACGCT GGCGAGCACG CCGAGGCCAT CGCCAGCCGC 
GAGCAGGAGG TGTTGCAGGG CTGGAAGGAG CTGCTAGCAG CCTGTGAAGA TGCTCGTCTG 
CACGTCAGCT CCACGGCCGA CGCCCTGCGC TTCCACAGCC AGGCCCGCGA CCTGCTCTCC 
TGGATGGACG GCATCGCGGG CCAGATCGGG GCAGCTGACA AACCCAGGGA TGTGTCATCG 
GTGGAGGTGC TTATGAACTA CCACCAGGGC CTGAAGACAG AGTTGGAGGC GCGCGTGCCT 
GAGCTGGCAA CCTGCCAGGA GCTGGGGCGG TCTCTATTAC TCAACAAAAG CGCCATGGCT 
GATGAGATCC AGGCCCAGCT GGACAAGCTG GGAAGCCGGA AGGAGGAGGT GTCGGAGAAG 
TGGGACCGTC ACTGGGAGTG GCTGCAGCAG ATGCTGGAGG TACACCAGTT TGCTCAGGAG 
GCCGTGGTGG CTGACGCCTG GCTGACAGCT CAGGAACCGC TCCTGCAGAG CCGGGAGCTG 
GGCAGCAGTG TGGATGAGGT GGAGCAGCTT ATCCGGCGAC ACGAGGCCTT CCGAAAAGCA 
GCTGCGGCCT GGGAGGAGAG GTTCAGCTCT CTGCGGCGCT TGACCACGAT CGAGAAACTC 
AAGGCAGAAC AGAGTAAGCA GCCACCCACC CCCTTACTGG GGCGCAAGTT CTTTGGGGAC 
CCCACAGAGC TGGCGGCCAA GGCAGCGCCT TTGCTGCGTC CAGGGGGCTA TGACAGGGGC 
CTGGAGCCTT TGGCCCGCAG GGCCTCGGAC ACGCTGTCAG CGGAGGTGCG GACCCGGGTT 
GGGTATGTGC GCCAGGAGCT CAAGCCCGAG CGTCTCCAGC CGCGCATTGA CCGGCTGCCA 
GAGACCTCGG GGAAGGTAGA ACCCGCGGCC CCGACAGCCG CAGCGTTGGA CACGACGGAC 
ACCCCTGGGA CTCCGGCGGC GACGGAGTTG GTCCGGCCCC GGTCGGAGCG CCAGGAGTTA 
GCGGATCGTG CGGAGGAGCT GCCACGGAGA CGAAGATCAG AACGCCAGGA GTCCGTGGAT 
CAACCAGAGG AGACAGCGCG GAGGCGGCGG CCTGAGCGCC AGGAGTCCGC GGACCACGAG 
GGGCCGCACA GCCTTACTCT AGGTCGCTAC GAGCAGATGG AGAGACGGCG GGAGCGGAGG 
GAGCGGCGGA TAGAACGACA GGAGTCTAGC GAACAGGAGA CTCCCACCAG GGGAGAGCTG 
GTCAAAGGGA AGGCCACTCT GGCTGATATT GTGGAGCAAC TTCAGGAGAA AGAGGCAGGC 
CCAGGGATCC CTGCTGGGGT GCCGTCGCTG CCTCAGCCTC GCGAGCTTCC CCCTGGTCGC 
CTGCCCAACG GGCTTGAGCC GCCCGAGCGG ACACCTCGGC CCGATCGGCC GCGGGCACGG 
GACCGGCCCA AGCCGCGCCG GCGGCCACGG CCTCGAGAGG GTGGTGAGGG CGGGGGAAGC 
CGGCGCTCGC GCTCCGCCCC TGCCCAGGGA GGCTCCGCCC CCGCGCCGCC ACCTCCGCCC 
ACTCACACAG TGCAGCACGA GGGCTTCCTG CTGCGCAAGC GCGAGCTCGA CGCTAACCGC 
AAGTCGTCCA ACCGGTCATG GGTGAGCCTG TACTGTGTGC TCAGCAAGGG GGAGCTGGGC 
TTCTACAAGG ACTCCAAAGG CCCAGCATCA GGGGGCACGC ATGGTGGGGA ACCACTGCTC 
AGCCTGCACA AAGCCACCAG CGAGGTGGCT AGTGACTACA AGAAAAAGAA GCATGTCTTC 
AAGCTCCAGA CCCAGGATGG CAGTGAGTTC TTGCTCCAGG CTAAAGATGA GGAGGAGATG 
AACGGCTGGC TGGAGGCTGT GGCTAACTCC GTGGCAGAAC ACGCCGAGAT CGCCCGATGG 
GGTCAGACAC TACCTACCAC ATCATCCACA GATGAGGGCA ACCCCAAGCG TGAGGGCGGG 
GAGCGTAGGG CCAGCGGGCG CCGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540499.1
CDS	20..7849
Translation	MRIWPSGGPVGPNRAGPGPATNARREGGAARRASGTASAAGAQALPPPMAQVPGEVDNMEGPAVSNNNNPSARWESPDRGWDREPPAAANAAASLFECSRIKALADEREAVQKKTFTKWVNSHLARVGCHIGDLYADLRDGFVLTRLLEVLSGEQLPRPTRGRMRIHSLENVDKALQFLKEQRVHLENVGSHDIVDGNHRLTLGLVWTIILRFQIQVIKIETEDNRETRSAKDALLLWCQMKTAGYPEVNIQNFTTSWRDGLAFNALIHRHRPDLVDLSKLTKSNANYNLQRAFRTAEQHLGLARLLDPEDVNMEAPDEKSIITYVVSFYHYFSKMKALAVEGKRIGKVLDQVLEVDKIIERYEELAAELLAWIHRTVDLISNQKFANSLSGVQQQLQAFTAYCTLEKPVKFQEKGNLEVLLFSIQSKLRAHNRRLFVPREGCGIWDIDKAWGELEKAEHEREAALRAELIRQEKLELLAQRFDHKVAMRESWLNENQRLVSQDNFGYELPAVEAAMKKHEAIEADIAAYEERVQGVAELAQALAAEGYYDARRVAAQRDSVLRQWALLTGLVGARRTRLEQNLALQKVFQEMVYMVDWMEEMQTQLLSRECGQHLVEADDLLQKHGLLEGDIAAQSERVEALNAAALRFSQLQGYQPCDPQVICNRVNHVHGCLSELQEQAARRRAELEASRSLWALLQELEEAESWARDKERLLEATSGSGGAAGTAGGAHDLSSTARLLAQHKILQGELGGRRALLQQALRRGEELAAAGGSVGPGAEPLHLAGLAERAASARRRWQRLEEAAARRERRLQEARALHQFGADLDGLLDWLRDAYRLAAAGDFGHDEASSRRLARQHRALTGEVEAHRGPVGGLRRQLATLGGASGAGPLVVALQVRVVEAEQLFAEVTEVAALRRQWLRDALAVYRMFGEVHACELWIGEKEQWLLAMRVPDSLDDVEVVQHRFESLDQEMNSLMGRVLDVNQTVQELVEGGHPSSDEVRSCQDHLNSRWNRIVELVEQRKEEMSAVLLVENHVLEVAEVRAQVREKRRAVESAPRAGGALQWRLSGLEAALQALEPRQAALLEEAALLAERFPAQATRLHQGAEELGAEWGALAGAAQACGEAVAAAGRLQRFLRDLDTFLDWLVRAQEAAGAVEGPLPRSLEEADGLLARHAALKEEVDQREEDYARIVAASEALLASEGAELGPGLALDEWLPHLEVGWHKLLGLWEERREALVQAHVYQLFLRDLCQALAVLRNQEVALSGAELPCTVESVEEAMKRHRDFLTTMELNQQKMQVAVQAAESLLRQGNAYGEQAQEAVARLLEKSQENQLRAQQWMQKLLDQLVLQHFLRDCHELDGWIHEKMLMARDGTREDSHKLHKRWLRHQAFMAELAQNKEWLEKIEREGQQLMQEKPELAASVRKKLGEIRQCWAELESTTQAKARQLFEASKADQLVQSFAELDKRLLHMESQLQDVDPGGDLATVNSQLKKLQSMESQVEEWCREVGELQAQTAALPLEQASKELVGERQSAVGERLVRLLEPLQERRRLLLASKELHQVAHDLDDELAWVQERLPLAMQTERGTGLQAVQQHIKKNQGLRREIQAHGPRLEEVLERAGVLASLRSPEAEAVRRGQEQLQSAWTGLREAAERRQQTLDAAFQVEQYYFDVAEVEAWLGEQELLMMSEDKGKDEQSTLQLLKKHLQLEQGVENYEESIAQLSRQCRALLEMGHPDSEQISRRQSQVDRLYVALKELGEERRVSLEQQYWLYQLSRQVDELEHWIAEKEVVAGSPELGQDFEHVSVLQEKFSEFASETGTAGRERLAAVNQMVDELIECGHTAAATMAEWKDGLNEAWAELLELMGTRAQLLAASRELHKFFSDARELQGQIEEKRRRLPRLTAPPEPRPSASSMQRTLRAFEHDLQLLVSQVRQLQEGAAQLRTVYAGEHAEAIASREQEVLQGWKELLAACEDARLHVSSTADALRFHSQARDLLSWMDGIAGQIGAADKPRDVSSVEVLMNYHQGLKTELEARVPELATCQELGRSLLLNKSAMADEIQAQLDKLGSRKEEVSEKWDRHWEWLQQMLEVHQFAQEAVVADAWLTAQEPLLQSRELGSSVDEVEQLIRRHEAFRKAAAAWEERFSSLRRLTTIEKLKAEQSKQPPTPLLGRKFFGDPTELAAKAAPLLRPGGYDRGLEPLARRASDTLSAEVRTRVGYVRQELKPERLQPRIDRLPETSGKVEPAAPTAAALDTTDTPGTPAATELVRPRSERQELADRAEELPRRRRSERQESVDQPEETARRRRPERQESADHEGPHSLTLGRYEQMERRRERRERRIERQESSEQETPTRGELVKGKATLADIVEQLQEKEAGPGIPAGVPSLPQPRELPPGRLPNGLEPPERTPRPDRPRARDRPKPRRRPRPREGGEGGGSRRSRSAPAQGGSAPAPPPPPTHTVQHEGFLLRKRELDANRKSSNRSWVSLYCVLSKGELGFYKDSKGPASGGTHGGEPLLSLHKATSEVASDYKKKKHVFKLQTQDGSEFLLQAKDEEEMNGWLEAVANSVAEHAEIARWGQTLPTTSSTDEGNPKREGGERRASGRRK

Target ORF information:

RefSeq Version	XM_006540436.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540436.3

ORF Insert Sequence:

ATGCGGATCT GGCCGAGCGG CGGGCCGGTG GGGCCGAACA GAGCCGGGCC CGGCCCGGCG 
ACGAACGCCC GCAGGGAGGG CGGTGCTGCG CGGCGAGCAT CCGGGACCGC ATCGGCGGCC 
GGCGCCCAGG CCCTGCCTCC CCCAATGGCA CAAGTACCAG GGGAGGTGGA CAACATGGAG 
GGCCCAGCAG TCTCCAACAA CAACAACCCT TCAGCTCGCT GGGAGAGCCC AGATCGGGGC 
TGGGATCGGG AACCTCCCGC AGCCGCCAAT GCTGCGGCTT CACTCTTTGA GTGCTCTCGG 
ATCAAGGCTT TAGCAGATGA GCGAGAGGCT GTGCAGAAGA AAACCTTCAC CAAGTGGGTG 
AATTCGCACC TTGCCCGCGT GGGCTGCCAC ATTGGAGACC TGTATGCAGA CCTTCGGGAC 
GGGTTTGTCC TCACCCGGCT TCTGGAAGTG CTGTCGGGGG AGCAGCTGCC GAGGCCCACT 
CGCGGCCGCA TGCGGATCCA CTCGCTGGAG AACGTGGACA AGGCTCTGCA GTTCCTGAAG 
GAGCAGCGTG TGCATCTGGA GAACGTGGGG TCACATGACA TCGTGGATGG GAACCACCGA 
CTGACGCTGG GGCTGGTCTG GACCATCATC CTGCGCTTCC AGATTCAAGT CATCAAGATT 
GAGACAGAAG ACAACAGAGA GACCCGCTCC GCCAAGGACG CTCTGCTCTT GTGGTGCCAG 
ATGAAAACAG CCGGTTACCC TGAGGTAAAC ATCCAGAATT TCACCACCAG CTGGAGAGAT 
GGCCTGGCCT TCAATGCCCT CATTCACCGT CACAGGCCTG ATCTTGTGGA CCTCAGCAAA 
CTTACCAAGT CCAACGCCAA CTACAACCTG CAGAGAGCTT TCCGCACAGC CGAGCAGCAC 
CTGGGGCTAG CACGTCTGCT GGACCCCGAA GATGTGAACA TGGAGGCTCC AGATGAGAAG 
TCCATTATCA CCTATGTGGT CTCCTTCTAC CACTACTTCT CCAAGATGAA GGCTCTGGCG 
GTGGAGGGAA AACGGATTGG AAAGGTCCTG GACCAGGTGT TGGAGGTGGA CAAGATCATA 
GAACGCTATG AGGAGCTGGC GGCGGAGCTG TTGGCCTGGA TCCATCGCAC TGTGGACCTC 
ATTAGCAACC AAAAGTTTGC TAACTCACTG AGTGGGGTGC AGCAGCAGCT GCAGGCCTTC 
ACGGCTTACT GTACGCTGGA GAAGCCCGTC AAGTTTCAGG AGAAGGGGAA CCTGGAGGTG 
CTACTCTTCA GCATCCAAAG CAAACTGCGC GCCCACAACC GGCGCCTCTT TGTGCCTCGA 
GAAGGCTGTG GCATCTGGGA TATTGACAAG GCGTGGGGTG AGCTGGAGAA GGCAGAGCAT 
GAGCGAGAGG CGGCCCTCAG AGCTGAGCTC ATCCGGCAGG AGAAGTTGGA ACTCTTGGCT 
CAAAGATTTG ACCACAAGGT GGCCATGAGG GAGAGCTGGC TAAATGAGAA CCAGCGCCTG 
GTCTCCCAGG ACAACTTCGG ATATGAGCTG CCGGCAGTGG AGGCAGCCAT GAAGAAGCAC 
GAAGCCATTG AGGCAGACAT TGCAGCCTAC GAGGAGCGGG TTCAGGGTGT GGCGGAGTTA 
GCCCAGGCGT TGGCAGCTGA AGGCTACTAT GATGCCCGGC GGGTGGCTGC CCAGCGTGAC 
AGTGTCCTTC GCCAGTGGGC CCTGCTGACC GGGCTGGTAG GTGCCCGGCG GACCCGGCTT 
GAGCAGAATC TGGCCCTGCA GAAGGTCTTC CAGGAGATGG TCTACATGGT GGACTGGATG 
GAGGAGATGC AGACTCAGCT GCTGTCTCGG GAATGTGGGC AGCACCTGGT GGAGGCCGAT 
GACCTGCTGC AGAAGCACGG GCTGCTGGAG GGGGACATCG CAGCCCAGAG TGAGCGAGTG 
GAGGCCCTCA ATGCTGCAGC CCTGAGATTC TCCCAGCTAC AGGGCTATCA GCCCTGCGAC 
CCTCAGGTCA TTTGCAACCG TGTGAACCAC GTGCATGGCT GTCTGTCGGA GCTGCAGGAG 
CAGGCAGCAC GGCGGCGCGC AGAGCTGGAA GCTTCTCGGA GCCTGTGGGC GCTGCTGCAG 
GAGCTGGAGG AGGCGGAGAG TTGGGCGCGG GACAAGGAGC GCCTCCTAGA GGCCACGAGC 
GGCAGTGGTG GCGCGGCGGG CACGGCGGGC GGCGCGCACG ACCTGTCCAG CACCGCTCGC 
CTGCTGGCGC AGCATAAGAT CCTGCAGGGC GAGCTGGGCG GCCGGCGGGC GCTGCTGCAG 
CAGGCGCTGC GACGCGGGGA GGAGTTGGCT GCGGCGGGCG GCTCCGTGGG CCCGGGCGCC 
GAGCCTTTGC ATTTGGCTGG CCTGGCGGAG CGCGCGGCTA GCGCTCGGCG CCGCTGGCAG 
CGGCTGGAAG AGGCGGCTGC GCGCCGGGAA CGGCGGCTGC AGGAGGCGCG GGCGCTGCAC 
CAATTTGGCG CCGACCTGGA CGGGCTGCTG GACTGGCTTC GTGACGCGTA TCGTCTAGCA 
GCCGCTGGAG ATTTTGGTCA CGACGAAGCG TCCAGCCGTC GCCTGGCGCG CCAGCACCGC 
GCTCTCACCG GGGAGGTGGA AGCGCACCGT GGCCCTGTGG GTGGCCTGAG GCGCCAGCTG 
GCGACACTGG GAGGAGCCAG TGGCGCAGGG CCGCTGGTGG TGGCTCTGCA GGTGCGCGTG 
GTGGAAGCCG AACAGTTGTT CGCCGAGGTG ACCGAGGTGG CCGCGCTGCG ACGACAGTGG 
CTTCGGGACG CGCTCGCCGT GTACCGCATG TTCGGCGAGG TACATGCCTG CGAGTTGTGG 
ATCGGGGAGA AAGAGCAGTG GCTGCTGGCC ATGCGTGTGC CCGATTCCCT CGACGACGTT 
GAGGTGGTGC AGCATCGATT TGAAAGTCTT GACCAGGAAA TGAACAGCCT GATGGGCCGA 
GTCCTGGATG TGAACCAAAC AGTTCAGGAG TTGGTAGAAG GAGGTCATCC CAGCTCCGAT 
GAGGTGCGCT CCTGTCAGGA CCATCTCAAC AGCAGGTGGA ACCGCATCGT GGAGTTGGTG 
GAACAGCGCA AAGAGGAGAT GAGCGCGGTG CTGCTGGTGG AGAACCACGT GCTGGAGGTG 
GCCGAGGTGC GCGCCCAAGT GCGCGAGAAG CGACGGGCAG TGGAGAGCGC GCCCCGGGCG 
GGGGGAGCCC TGCAATGGCG CCTGAGTGGC CTGGAGGCGG CTCTACAGGC GCTAGAGCCG 
CGACAGGCGG CGCTGCTGGA GGAGGCGGCG CTGCTGGCCG AACGCTTCCC CGCACAGGCG 
ACGCGCCTGC ACCAGGGCGC CGAGGAGCTA GGAGCCGAGT GGGGCGCACT GGCCGGAGCG 
GCTCAGGCCT GCGGCGAGGC GGTTGCGGCG GCAGGTCGCC TGCAGCGTTT CCTGCGCGAT 
TTGGATACTT TCCTGGACTG GCTGGTGCGG GCGCAGGAGG CAGCAGGCGC CGTCGAGGGG 
CCGCTGCCTC GCAGCCTGGA GGAGGCGGAT GGGCTGCTGG CGCGCCACGC CGCGCTCAAG 
GAGGAGGTGG ATCAGCGTGA GGAGGACTAC GCGCGCATCG TGGCTGCCAG TGAAGCGCTG 
CTAGCGTCCG AGGGCGCGGA GCTGGGCCCC GGTCTGGCGC TAGACGAGTG GCTGCCGCAC 
CTCGAGGTTG GCTGGCACAA ACTGCTCGGC TTGTGGGAGG AGCGCAGGGA GGCGCTGGTC 
CAAGCGCACG TCTATCAACT CTTCCTGAGA GACCTGTGCC AGGCGCTTGC GGTGCTGCGC 
AACCAGGAGG TGGCGCTGTC TGGCGCTGAA CTCCCTTGCA CAGTGGAGTC GGTAGAAGAG 
GCGATGAAAA GGCACCGGGA TTTTCTCACC ACGATGGAGC TGAACCAGCA AAAGATGCAG 
GTGGCGGTTC AAGCTGCAGA GAGCCTGCTG CGGCAAGGCA ACGCCTACGG GGAGCAGGCG 
CAGGAGGCAG TGGCTCGGCT GCTAGAGAAG AGCCAAGAAA ATCAGCTGAG GGCCCAGCAG 
TGGATGCAAA AACTACTGGA CCAGCTTGTG CTGCAGCACT TTCTTAGAGA TTGTCATGAG 
CTGGATGGCT GGATCCATGA GAAGATGCTG ATGGCTCGTG ATGGCACCCG GGAAGACAGC 
CACAAGCTGC ACAAGAGATG GCTCCGGCAC CAGGCGTTCA TGGCTGAGCT GGCTCAGAAT 
AAGGAGTGGC TGGAGAAGAT AGAAAGGGAG GGCCAGCAAC TGATGCAGGA GAAACCGGAG 
CTGGCAGCCT CTGTGCGCAA GAAATTAGGT GAGATCCGGC AGTGCTGGGC GGAGCTGGAG 
AGCACCACAC AGGCCAAGGC GCGCCAGCTC TTCGAGGCCA GCAAGGCGGA CCAGCTAGTG 
CAGAGCTTCG CGGAGCTGGA CAAGCGGCTG CTTCACATGG AAAGCCAGCT GCAAGACGTA 
GACCCCGGTG GGGACCTGGC CACTGTCAAC AGCCAGCTCA AGAAGTTACA GTCCATGGAG 
TCTCAGGTGG AGGAGTGGTG CCGAGAGGTG GGCGAGCTTC AGGCGCAGAC GGCGGCGCTT 
CCGCTGGAGC AGGCGAGCAA GGAGTTGGTG GGGGAGCGGC AGAGTGCGGT GGGCGAGCGC 
CTGGTGCGCC TGCTGGAGCC TTTGCAGGAG CGACGCCGCT TGTTGCTGGC ATCCAAGGAG 
CTGCATCAGG TGGCCCACGA CCTGGACGAT GAACTGGCTT GGGTTCAAGA GCGGCTGCCA 
CTGGCCATGC AGACAGAGCG AGGCACTGGC TTGCAGGCTG TCCAGCAGCA CATCAAAAAG 
AACCAGGGCC TGCGGCGGGA GATCCAGGCT CACGGGCCGC GCTTGGAGGA GGTGCTGGAG 
CGCGCGGGCG TGCTGGCCTC GCTGCGCAGC CCAGAGGCCG AAGCGGTGCG CCGTGGCCAA 
GAGCAGCTGC AGAGCGCCTG GACCGGGCTG CGGGAAGCGG CCGAACGGCG GCAGCAGACG 
CTGGACGCCG CCTTCCAGGT GGAACAGTAC TACTTTGACG TGGCTGAGGT GGAGGCATGG 
CTGGGCGAGC AGGAGCTGCT CATGATGAGT GAGGACAAGG GCAAGGATGA ACAGAGCACC 
CTGCAGCTGC TCAAGAAGCA TTTACAACTG GAACAGGGCG TGGAGAACTA TGAGGAAAGC 
ATCGCCCAGC TGTCACGCCA GTGCCGGGCG CTACTGGAAA TGGGACACCC AGACAGTGAG 
CAAATCAGCC GCCGACAGTC CCAAGTGGAT CGCCTCTATG TGGCGCTCAA GGAGCTGGGC 
GAGGAACGCA GGGTGAGCCT GGAACAGCAG TACTGGCTCT ACCAGCTCAG CCGCCAGGTG 
GATGAGCTGG AACACTGGAT AGCCGAGAAG GAGGTGGTAG CTGGCTCCCC AGAGCTGGGC 
CAGGACTTCG AACACGTGTC GGTGCTACAG GAGAAATTCT CAGAGTTTGC CAGTGAGACA 
GGAACCGCAG GGCGGGAGCG GCTGGCGGCG GTCAACCAGA TGGTGGACGA GCTGATTGAG 
TGTGGTCACA CAGCAGCGGC CACCATGGCT GAGTGGAAGG ACGGGCTGAA CGAGGCCTGG 
GCTGAGCTGC TGGAACTCAT GGGCACTCGG GCCCAGCTGC TCGCTGCCTC TCGGGAGCTG 
CATAAGTTCT TCAGCGATGC CCGGGAGCTT CAAGGGCAGA TTGAGGAGAA GCGGAGGAGG 
CTGCCCCGCC TGACGGCACC ACCTGAACCC AGACCCAGTG CCAGCTCCAT GCAGCGGACC 
CTGCGAGCCT TTGAGCACGA CCTGCAGCTC CTCGTGTCCC AGGTACGGCA GCTGCAGGAA 
GGGGCGGCCC AGCTGCGGAC GGTGTACGCT GGCGAGCACG CCGAGGCCAT CGCCAGCCGC 
GAGCAGGAGG TGTTGCAGGG CTGGAAGGAG CTGCTAGCAG CCTGTGAAGA TGCTCGTCTG 
CACGTCAGCT CCACGGCCGA CGCCCTGCGC TTCCACAGCC AGGCCCGCGA CCTGCTCTCC 
TGGATGGACG GCATCGCGGG CCAGATCGGG GCAGCTGACA AACCCAGGGA TGTGTCATCG 
GTGGAGGTGC TTATGAACTA CCACCAGGGC CTGAAGACAG AGTTGGAGGC GCGCGTGCCT 
GAGCTGGCAA CCTGCCAGGA GCTGGGGCGG TCTCTATTAC TCAACAAAAG CGCCATGGCT 
GATGAGATCC AGGCCCAGCT GGACAAGCTG GGAAGCCGGA AGGAGGAGGT GTCGGAGAAG 
TGGGACCGTC ACTGGGAGTG GCTGCAGCAG ATGCTGGAGG TACACCAGTT TGCTCAGGAG 
GCCGTGGTGG CTGACGCCTG GCTGACAGCT CAGGAACCGC TCCTGCAGAG CCGGGAGCTG 
GGCAGCAGTG TGGATGAGGT GGAGCAGCTT ATCCGGCGAC ACGAGGCCTT CCGAAAAGCA 
GCTGCGGCCT GGGAGGAGAG GTTCAGCTCT CTGCGGCGCT TGACCACGAT CGAGAAACTC 
AAGGCAGAAC AGAGTAAGCA GCCACCCACC CCCTTACTGG GGCGCAAGTT CTTTGGGGAC 
CCCACAGAGC TGGCGGCCAA GGCAGCGCCT TTGCTGCGTC CAGGGGGCTA TGACAGGGGC 
CTGGAGCCTT TGGCCCGCAG GGCCTCGGAC ACGCTGTCAG CGGAGGTGCG GACCCGGGTT 
GGGTATGTGC GCCAGGAGCT CAAGCCCGAG CGTCTCCAGC CGCGCATTGA CCGGCTGCCA 
GAGACCTCGG GGAAGGTAGA ACCCGCGGCC CCGACAGCCG CAGCGTTGGA CACGACGGAC 
ACCCCTGGGA CTCCGGCGGC GACGGAGTTG GTCCGGCCCC GGTCGGAGCG CCAGGAGTTA 
GCGGATCGTG CGGAGGAGCT GCCACGGAGA CGAAGATCAG AACGCCAGGA GTCCGTGGAT 
CAACCAGAGG AGACAGCGCG GAGGCGGCGG CCTGAGCGCC AGGAGTCCGC GGACCACGAG 
GGGCCGCACA GCCTTACTCT AGGTCGCTAC GAGCAGATGG AGAGACGGCG GGAGCGGAGG 
GAGCGGCGGA TAGAACGACA GGAGTCTAGC GAACAGGAGA CTCCCACCAG GGGAGAGCTG 
GTCAAAGGGA AGGCCACTCT GGCTGATATT GTGGAGCAAC TTCAGGAGAA AGAGGCAGGC 
CCAGGGATCC CTGCTGGGGT GCCGTCGCTG CCTCAGCCTC GCGAGCTTCC CCCTGGTCGC 
CTGCCCAACG GGCTTGAGCC GCCCGAGCGG ACACCTCGGC CCGATCGGCC GCGGGCACGG 
GACCGGCCCA AGCCGCGCCG GCGGCCACGG CCTCGAGAGG GTGGTGAGGG CGGGGGAAGC 
CGGCGCTCGC GCTCCGCCCC TGCCCAGGGA GGCTCCGCCC CCGCGCCGCC ACCTCCGCCC 
ACTCACACAG TGCAGCACGA GGGCTTCCTG CTGCGCAAGC GCGAGCTCGA CGCTAACCGC 
AAGTCGTCCA ACCGGTCATG GGTGAGCCTG TACTGTGTGC TCAGCAAGGG GGAGCTGGGC 
TTCTACAAGG ACTCCAAAGG CCCAGCATCA GGGGGCACGC ATGGTGGGGA ACCACTGCTC 
AGCCTGCACA AAGCCACCAG CGAGGTGGCT AGTGACTACA AGAAAAAGAA GCATGTCTTC 
AAGCTCCAGA CCCAGGATGG CAGTGAGTTC TTGCTCCAGG CTAAAGATGA GGAGGAGATG 
AACGGCTGGC TGGAGGCTGT GGCTAACTCC GTGGCAGAAC ACGCCGAGAT CGCCCGATGG 
GGTCAGACAC TACCTACCAC ATCATCCACA GATGAGGGCA ACCCCAAGCG TGAGGGCGGG 
GAGCGTAGGG CCAGCGGGCG CCGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn4 ( NM_001199234.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001186163.1 Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant sigma6a, mRNA.

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CloneID	OMu02126
Clone ID Related Accession (Same CDS sequence)	NM_001199234.1 , NM_001199235.1 , NM_001199236.1
Accession Version	NM_001199234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 4 isoform sigma6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC157561.10. Transcript Variant: This variant (sigma6a) has a shorter and distinct 5' terminus which results in the use of an in-frame downstream translation initiation signal, compared to sigma1. Compared to isoform sigma1, it encodes an isoform (sigma6) that lacks multiple calponin-homology domains and spectrin repeats at the N-terminus. Variants sigma6a-c encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments to AB055619.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB055619.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGCTGATGG CTCGTGATGG CACCCGGGAA GACAGCCACA AGCTGCACAA GAGATGGCTC 
CGGCACCAGG CGTTCATGGC TGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATAGAA 
AGGGAGGGCC AGCAACTGAT GCAGGAGAAA CCGGAGCTGG CAGCCTCTGT GCGCAAGAAA 
TTAGGTGAGA TCCGGCAGTG CTGGGCGGAG CTGGAGAGCA CCACACAGGC CAAGGCGCGC 
CAGCTCTTCG AGGCCAGCAA GGCGGACCAG CTAGTGCAGA GCTTCGCGGA GCTGGACAAG 
CGGCTGCTTC ACATGGAAAG CCAGCTGCAA GACGTAGACC CCGGTGGGGA CCTGGCCACT 
GTCAACAGCC AGCTCAAGAA GTTACAGTCC ATGGAGTCTC AGGTGGAGGA GTGGTGCCGA 
GAGGTGGGCG AGCTTCAGGC GCAGACGGCG GCGCTTCCGC TGGAGCAGGC GAGCAAGGAG 
TTGGTGGGGG AGCGGCAGAG TGCGGTGGGC GAGCGCCTGG TGCGCCTGCT GGAGCCTTTG 
CAGGAGCGAC GCCGCTTGTT GCTGGCATCC AAGGAGCTGC ATCAGGTGGC CCACGACCTG 
GACGATGAAC TGGCTTGGGT TCAAGAGCGG CTGCCACTGG CCATGCAGAC AGAGCGAGGC 
ACTGGCTTGC AGGCTGTCCA GCAGCACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC 
CAGGCTCACG GGCCGCGCTT GGAGGAGGTG CTGGAGCGCG CGGGCGTGCT GGCCTCGCTG 
CGCAGCCCAG AGGCCGAAGC GGTGCGCCGT GGCCAAGAGC AGCTGCAGAG CGCCTGGACC 
GGGCTGCGGG AAGCGGCCGA ACGGCGGCAG CAGACGCTGG ACGCCGCCTT CCAGGTGGAA 
CAGTACTACT TTGACGTGGC TGAGGTGGAG GCATGGCTGG GCGAGCAGGA GCTGCTCATG 
ATGAGTGAGG ACAAGGGCAA GGATGAACAG AGCACCCTGC AGCTGCTCAA GAAGCATTTA 
CAACTGGAAC AGGGCGTGGA GAACTATGAG GAAAGCATCG CCCAGCTGTC ACGCCAGTGC 
CGGGCGCTAC TGGAAATGGG ACACCCAGAC AGTGAGCAAA TCAGCCGCCG ACAGTCCCAA 
GTGGATCGCC TCTATGTGGC GCTCAAGGAG CTGGGCGAGG AACGCAGGGT GAGCCTGGAA 
CAGCAGTACT GGCTCTACCA GCTCAGCCGC CAGGTGGATG AGCTGGAACA CTGGATAGCC 
GAGAAGGAGG TGGTAGCTGG CTCCCCAGAG CTGGGCCAGG ACTTCGAACA CGTGTCGGTG 
CTACAGGAGA AATTCTCAGA GTTTGCCAGT GAGACAGGAA CCGCAGGGCG GGAGCGGCTG 
GCGGCGGTCA ACCAGATGGT GGACGAGCTG ATTGAGTGTG GTCACACAGC AGCGGCCACC 
ATGGCTGAGT GGAAGGACGG GCTGAACGAG GCCTGGGCTG AGCTGCTGGA ACTCATGGGC 
ACTCGGGCCC AGCTGCTCGC TGCCTCTCGG GAGCTGCATA AGTTCTTCAG CGATGCCCGG 
GAGCTTCAAG GGCAGATTGA GGAGAAGCGG AGGAGGCTGC CCCGCCTGAC GGCACCACCT 
GAACCCAGAC CCAGTGCCAG CTCCATGCAG CGGACCCTGC GAGCCTTTGA GCACGACCTG 
CAGCTCCTCG TGTCCCAGGT ACGGCAGCTG CAGGAAGGGG CGGCCCAGCT GCGGACGGTG 
TACGCTGGCG AGCACGCCGA GGCCATCGCC AGCCGCGAGC AGGAGGTGTT GCAGGGCTGG 
AAGGAGCTGC TAGCAGCCTG TGAAGATGCT CGTCTGCACG TCAGCTCCAC GGCCGACGCC 
CTGCGCTTCC ACAGCCAGGC CCGCGACCTG CTCTCCTGGA TGGACGGCAT CGCGGGCCAG 
ATCGGGGCAG CTGACAAACC CAGGGATGTG TCATCGGTGG AGGTGCTTAT GAACTACCAC 
CAGGGCCTGA AGACAGAGTT GGAGGCGCGC GTGCCTGAGC TGGCAACCTG CCAGGAGCTG 
GGGCGGTCTC TATTACTCAA CAAAAGCGCC ATGGCTGATG AGATCCAGGC CCAGCTGGAC 
AAGCTGGGAA GCCGGAAGGA GGAGGTGTCG GAGAAGTGGG ACCGTCACTG GGAGTGGCTG 
CAGCAGATGC TGGAGGTACA CCAGTTTGCT CAGGAGGCCG TGGTGGCTGA CGCCTGGCTG 
ACAGCTCAGG AACCGCTCCT GCAGAGCCGG GAGCTGGGCA GCAGTGTGGA TGAGGTGGAG 
CAGCTTATCC GGCGACACGA GGCCTTCCGA AAAGCAGCTG CGGCCTGGGA GGAGAGGTTC 
AGCTCTCTGC GGCGCTTGAC CACGATCGAG AAACTCAAGG CAGAACAGAG TAAGCAGCCA 
CCCACCCCCT TACTGGGGCG CAAGTTCTTT GGGGACCCCA CAGAGCTGGC GGCCAAGGCA 
GCGCCTTTGC TGCGTCCAGG GGGCTATGAC AGGGGCCTGG AGCCTTTGGC CCGCAGGGCC 
TCGGACACGC TGTCAGCGGA GGTGCGGACC CGGGTTGGGT ATGTGCGCCA GGAGCTCAAG 
CCCGAGCGTC TCCAGCCGCG CATTGACCGG CTGCCAGAGA CCTCGGGGAA GGTAGAACCC 
GCGGCCCCGA CAGCCGCAGC GTTGGACACG ACGGACACCC CTGGGACTCC GGCGGCGACG 
GAGTTGGTCC GGCCCCGGTC GGAGCGCCAG GAGTTAGCGG ATCGTGCGGA GGAGCTGCCA 
CGGAGACGAA GATCAGAACG CCAGGAGTCC GTGGATCAAC CAGAGGAGAC AGCGCGGAGG 
CGGCGGCCTG AGCGCCAGGA GTCCGCGGAC CACGAGGGGC CGCACAGCCT TACTCTAGGT 
CGCTACGAGC AGATGGAGAG ACGGCGGGAG CGGAGGGAGC GGCGGATAGA ACGACAGGAG 
TCTAGCGAAC AGGAGACTCC CACCAGGGGA GAGCTGGTCA AAGGGAAGGC CACTCTGGCT 
GATATTGTGG AGCAACTTCA GGAGAAAGAG GCAGGCCCAG GGATCCCTGC TGGGGTGCCG 
TCGCTGCCTC AGCCTCGCGA GCTTCCCCCT GGTCGCCTGC CCAACGGGCT TGAGCCGCCC 
GAGCGGACAC CTCGGCCCGA TCGGCCGCGG GCACGGGACC GGCCCAAGCC GCGCCGGCGG 
CCACGGCCTC GAGAGGGTGG TGAGGGCGGG GGAAGCCGGC GCTCGCGCTC CGCCCCTGCC 
CAGGGAGGCT CCGCCCCCGC GCCGCCACCT CCGCCCACTC ACACAGTGCA GCACGAGGGC 
TTCCTGCTGC GCAAGCGCGA GCTCGACGCT AACCGCAAGT CGTCCAACCG GTCATGGGTG 
AGCCTGTACT GTGTGCTCAG CAAGGGGGAG CTGGGCTTCT ACAAGGACTC CAAAGGCCCA 
GCATCAGGGG GCACGCATGG TGGGGAACCA CTGCTCAGCC TGCACAAAGC CACCAGCGAG 
GTGGCTAGTG ACTACAAGAA AAAGAAGCAT GTCTTCAAGC TCCAGACCCA GGATGGCAGT 
GAGTTCTTGC TCCAGGCTAA AGATGAGGAG GAGATGAACG GCTGGCTGGA GGCTGTGGCT 
AACTCCGTGG CAGAACACGC CGAGATCGCC CGATGGGGTC AGACACTACC TACCACATCA 
TCCACAGATG AGGGCAACCC CAAGCGTGAG GGCGGGGAGC GTAGGGCCAG CGGGCGCCGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186163.1
CDS	106..3831
Translation	MLMARDGTREDSHKLHKRWLRHQAFMAELAQNKEWLEKIEREGQQLMQEKPELAASVRKKLGEIRQCWAELESTTQAKARQLFEASKADQLVQSFAELDKRLLHMESQLQDVDPGGDLATVNSQLKKLQSMESQVEEWCREVGELQAQTAALPLEQASKELVGERQSAVGERLVRLLEPLQERRRLLLASKELHQVAHDLDDELAWVQERLPLAMQTERGTGLQAVQQHIKKNQGLRREIQAHGPRLEEVLERAGVLASLRSPEAEAVRRGQEQLQSAWTGLREAAERRQQTLDAAFQVEQYYFDVAEVEAWLGEQELLMMSEDKGKDEQSTLQLLKKHLQLEQGVENYEESIAQLSRQCRALLEMGHPDSEQISRRQSQVDRLYVALKELGEERRVSLEQQYWLYQLSRQVDELEHWIAEKEVVAGSPELGQDFEHVSVLQEKFSEFASETGTAGRERLAAVNQMVDELIECGHTAAATMAEWKDGLNEAWAELLELMGTRAQLLAASRELHKFFSDARELQGQIEEKRRRLPRLTAPPEPRPSASSMQRTLRAFEHDLQLLVSQVRQLQEGAAQLRTVYAGEHAEAIASREQEVLQGWKELLAACEDARLHVSSTADALRFHSQARDLLSWMDGIAGQIGAADKPRDVSSVEVLMNYHQGLKTELEARVPELATCQELGRSLLLNKSAMADEIQAQLDKLGSRKEEVSEKWDRHWEWLQQMLEVHQFAQEAVVADAWLTAQEPLLQSRELGSSVDEVEQLIRRHEAFRKAAAAWEERFSSLRRLTTIEKLKAEQSKQPPTPLLGRKFFGDPTELAAKAAPLLRPGGYDRGLEPLARRASDTLSAEVRTRVGYVRQELKPERLQPRIDRLPETSGKVEPAAPTAAALDTTDTPGTPAATELVRPRSERQELADRAEELPRRRRSERQESVDQPEETARRRRPERQESADHEGPHSLTLGRYEQMERRRERRERRIERQESSEQETPTRGELVKGKATLADIVEQLQEKEAGPGIPAGVPSLPQPRELPPGRLPNGLEPPERTPRPDRPRARDRPKPRRRPRPREGGEGGGSRRSRSAPAQGGSAPAPPPPPTHTVQHEGFLLRKRELDANRKSSNRSWVSLYCVLSKGELGFYKDSKGPASGGTHGGEPLLSLHKATSEVASDYKKKKHVFKLQTQDGSEFLLQAKDEEEMNGWLEAVANSVAEHAEIARWGQTLPTTSSTDEGNPKREGGERRASGRRK

Target ORF information:

RefSeq Version	NM_001199234.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant sigma6a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199234.1

ORF Insert Sequence:

ATGCTGATGG CTCGTGATGG CACCCGGGAA GACAGCCACA AGCTGCACAA GAGATGGCTC 
CGGCACCAGG CGTTCATGGC TGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATAGAA 
AGGGAGGGCC AGCAACTGAT GCAGGAGAAA CCGGAGCTGG CAGCCTCTGT GCGCAAGAAA 
TTAGGTGAGA TCCGGCAGTG CTGGGCGGAG CTGGAGAGCA CCACACAGGC CAAGGCGCGC 
CAGCTCTTCG AGGCCAGCAA GGCGGACCAG CTAGTGCAGA GCTTCGCGGA GCTGGACAAG 
CGGCTGCTTC ACATGGAAAG CCAGCTGCAA GACGTAGACC CCGGTGGGGA CCTGGCCACT 
GTCAACAGCC AGCTCAAGAA GTTACAGTCC ATGGAGTCTC AGGTGGAGGA GTGGTGCCGA 
GAGGTGGGCG AGCTTCAGGC GCAGACGGCG GCGCTTCCGC TGGAGCAGGC GAGCAAGGAG 
TTGGTGGGGG AGCGGCAGAG TGCGGTGGGC GAGCGCCTGG TGCGCCTGCT GGAGCCTTTG 
CAGGAGCGAC GCCGCTTGTT GCTGGCATCC AAGGAGCTGC ATCAGGTGGC CCACGACCTG 
GACGATGAAC TGGCTTGGGT TCAAGAGCGG CTGCCACTGG CCATGCAGAC AGAGCGAGGC 
ACTGGCTTGC AGGCTGTCCA GCAGCACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC 
CAGGCTCACG GGCCGCGCTT GGAGGAGGTG CTGGAGCGCG CGGGCGTGCT GGCCTCGCTG 
CGCAGCCCAG AGGCCGAAGC GGTGCGCCGT GGCCAAGAGC AGCTGCAGAG CGCCTGGACC 
GGGCTGCGGG AAGCGGCCGA ACGGCGGCAG CAGACGCTGG ACGCCGCCTT CCAGGTGGAA 
CAGTACTACT TTGACGTGGC TGAGGTGGAG GCATGGCTGG GCGAGCAGGA GCTGCTCATG 
ATGAGTGAGG ACAAGGGCAA GGATGAACAG AGCACCCTGC AGCTGCTCAA GAAGCATTTA 
CAACTGGAAC AGGGCGTGGA GAACTATGAG GAAAGCATCG CCCAGCTGTC ACGCCAGTGC 
CGGGCGCTAC TGGAAATGGG ACACCCAGAC AGTGAGCAAA TCAGCCGCCG ACAGTCCCAA 
GTGGATCGCC TCTATGTGGC GCTCAAGGAG CTGGGCGAGG AACGCAGGGT GAGCCTGGAA 
CAGCAGTACT GGCTCTACCA GCTCAGCCGC CAGGTGGATG AGCTGGAACA CTGGATAGCC 
GAGAAGGAGG TGGTAGCTGG CTCCCCAGAG CTGGGCCAGG ACTTCGAACA CGTGTCGGTG 
CTACAGGAGA AATTCTCAGA GTTTGCCAGT GAGACAGGAA CCGCAGGGCG GGAGCGGCTG 
GCGGCGGTCA ACCAGATGGT GGACGAGCTG ATTGAGTGTG GTCACACAGC AGCGGCCACC 
ATGGCTGAGT GGAAGGACGG GCTGAACGAG GCCTGGGCTG AGCTGCTGGA ACTCATGGGC 
ACTCGGGCCC AGCTGCTCGC TGCCTCTCGG GAGCTGCATA AGTTCTTCAG CGATGCCCGG 
GAGCTTCAAG GGCAGATTGA GGAGAAGCGG AGGAGGCTGC CCCGCCTGAC GGCACCACCT 
GAACCCAGAC CCAGTGCCAG CTCCATGCAG CGGACCCTGC GAGCCTTTGA GCACGACCTG 
CAGCTCCTCG TGTCCCAGGT ACGGCAGCTG CAGGAAGGGG CGGCCCAGCT GCGGACGGTG 
TACGCTGGCG AGCACGCCGA GGCCATCGCC AGCCGCGAGC AGGAGGTGTT GCAGGGCTGG 
AAGGAGCTGC TAGCAGCCTG TGAAGATGCT CGTCTGCACG TCAGCTCCAC GGCCGACGCC 
CTGCGCTTCC ACAGCCAGGC CCGCGACCTG CTCTCCTGGA TGGACGGCAT CGCGGGCCAG 
ATCGGGGCAG CTGACAAACC CAGGGATGTG TCATCGGTGG AGGTGCTTAT GAACTACCAC 
CAGGGCCTGA AGACAGAGTT GGAGGCGCGC GTGCCTGAGC TGGCAACCTG CCAGGAGCTG 
GGGCGGTCTC TATTACTCAA CAAAAGCGCC ATGGCTGATG AGATCCAGGC CCAGCTGGAC 
AAGCTGGGAA GCCGGAAGGA GGAGGTGTCG GAGAAGTGGG ACCGTCACTG GGAGTGGCTG 
CAGCAGATGC TGGAGGTACA CCAGTTTGCT CAGGAGGCCG TGGTGGCTGA CGCCTGGCTG 
ACAGCTCAGG AACCGCTCCT GCAGAGCCGG GAGCTGGGCA GCAGTGTGGA TGAGGTGGAG 
CAGCTTATCC GGCGACACGA GGCCTTCCGA AAAGCAGCTG CGGCCTGGGA GGAGAGGTTC 
AGCTCTCTGC GGCGCTTGAC CACGATCGAG AAACTCAAGG CAGAACAGAG TAAGCAGCCA 
CCCACCCCCT TACTGGGGCG CAAGTTCTTT GGGGACCCCA CAGAGCTGGC GGCCAAGGCA 
GCGCCTTTGC TGCGTCCAGG GGGCTATGAC AGGGGCCTGG AGCCTTTGGC CCGCAGGGCC 
TCGGACACGC TGTCAGCGGA GGTGCGGACC CGGGTTGGGT ATGTGCGCCA GGAGCTCAAG 
CCCGAGCGTC TCCAGCCGCG CATTGACCGG CTGCCAGAGA CCTCGGGGAA GGTAGAACCC 
GCGGCCCCGA CAGCCGCAGC GTTGGACACG ACGGACACCC CTGGGACTCC GGCGGCGACG 
GAGTTGGTCC GGCCCCGGTC GGAGCGCCAG GAGTTAGCGG ATCGTGCGGA GGAGCTGCCA 
CGGAGACGAA GATCAGAACG CCAGGAGTCC GTGGATCAAC CAGAGGAGAC AGCGCGGAGG 
CGGCGGCCTG AGCGCCAGGA GTCCGCGGAC CACGAGGGGC CGCACAGCCT TACTCTAGGT 
CGCTACGAGC AGATGGAGAG ACGGCGGGAG CGGAGGGAGC GGCGGATAGA ACGACAGGAG 
TCTAGCGAAC AGGAGACTCC CACCAGGGGA GAGCTGGTCA AAGGGAAGGC CACTCTGGCT 
GATATTGTGG AGCAACTTCA GGAGAAAGAG GCAGGCCCAG GGATCCCTGC TGGGGTGCCG 
TCGCTGCCTC AGCCTCGCGA GCTTCCCCCT GGTCGCCTGC CCAACGGGCT TGAGCCGCCC 
GAGCGGACAC CTCGGCCCGA TCGGCCGCGG GCACGGGACC GGCCCAAGCC GCGCCGGCGG 
CCACGGCCTC GAGAGGGTGG TGAGGGCGGG GGAAGCCGGC GCTCGCGCTC CGCCCCTGCC 
CAGGGAGGCT CCGCCCCCGC GCCGCCACCT CCGCCCACTC ACACAGTGCA GCACGAGGGC 
TTCCTGCTGC GCAAGCGCGA GCTCGACGCT AACCGCAAGT CGTCCAACCG GTCATGGGTG 
AGCCTGTACT GTGTGCTCAG CAAGGGGGAG CTGGGCTTCT ACAAGGACTC CAAAGGCCCA 
GCATCAGGGG GCACGCATGG TGGGGAACCA CTGCTCAGCC TGCACAAAGC CACCAGCGAG 
GTGGCTAGTG ACTACAAGAA AAAGAAGCAT GTCTTCAAGC TCCAGACCCA GGATGGCAGT 
GAGTTCTTGC TCCAGGCTAA AGATGAGGAG GAGATGAACG GCTGGCTGGA GGCTGTGGCT 
AACTCCGTGG CAGAACACGC CGAGATCGCC CGATGGGGTC AGACACTACC TACCACATCA 
TCCACAGATG AGGGCAACCC CAAGCGTGAG GGCGGGGAGC GTAGGGCCAG CGGGCGCCGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn4 ( NM_001199235.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001186164.1 Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant sigma6b, mRNA.

Price & Availability↑

CloneID	OMu02126
Clone ID Related Accession (Same CDS sequence)	NM_001199234.1 , NM_001199235.1 , NM_001199236.1
Accession Version	NM_001199235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 4 isoform sigma6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC157561.10. Transcript Variant: This variant (sigma6b) has a shorter and distinct 5' terminus which results in the use of an in-frame downstream translation initiation signal, compared to sigma1. Compared to isoform sigma1, it encodes an isoform (sigma6) that lacks multiple calponin-homology domains and spectrin repeats at the N-terminus. Variants sigma6a-c encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments to AB055620.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB055620.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGCTGATGG CTCGTGATGG CACCCGGGAA GACAGCCACA AGCTGCACAA GAGATGGCTC 
CGGCACCAGG CGTTCATGGC TGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATAGAA 
AGGGAGGGCC AGCAACTGAT GCAGGAGAAA CCGGAGCTGG CAGCCTCTGT GCGCAAGAAA 
TTAGGTGAGA TCCGGCAGTG CTGGGCGGAG CTGGAGAGCA CCACACAGGC CAAGGCGCGC 
CAGCTCTTCG AGGCCAGCAA GGCGGACCAG CTAGTGCAGA GCTTCGCGGA GCTGGACAAG 
CGGCTGCTTC ACATGGAAAG CCAGCTGCAA GACGTAGACC CCGGTGGGGA CCTGGCCACT 
GTCAACAGCC AGCTCAAGAA GTTACAGTCC ATGGAGTCTC AGGTGGAGGA GTGGTGCCGA 
GAGGTGGGCG AGCTTCAGGC GCAGACGGCG GCGCTTCCGC TGGAGCAGGC GAGCAAGGAG 
TTGGTGGGGG AGCGGCAGAG TGCGGTGGGC GAGCGCCTGG TGCGCCTGCT GGAGCCTTTG 
CAGGAGCGAC GCCGCTTGTT GCTGGCATCC AAGGAGCTGC ATCAGGTGGC CCACGACCTG 
GACGATGAAC TGGCTTGGGT TCAAGAGCGG CTGCCACTGG CCATGCAGAC AGAGCGAGGC 
ACTGGCTTGC AGGCTGTCCA GCAGCACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC 
CAGGCTCACG GGCCGCGCTT GGAGGAGGTG CTGGAGCGCG CGGGCGTGCT GGCCTCGCTG 
CGCAGCCCAG AGGCCGAAGC GGTGCGCCGT GGCCAAGAGC AGCTGCAGAG CGCCTGGACC 
GGGCTGCGGG AAGCGGCCGA ACGGCGGCAG CAGACGCTGG ACGCCGCCTT CCAGGTGGAA 
CAGTACTACT TTGACGTGGC TGAGGTGGAG GCATGGCTGG GCGAGCAGGA GCTGCTCATG 
ATGAGTGAGG ACAAGGGCAA GGATGAACAG AGCACCCTGC AGCTGCTCAA GAAGCATTTA 
CAACTGGAAC AGGGCGTGGA GAACTATGAG GAAAGCATCG CCCAGCTGTC ACGCCAGTGC 
CGGGCGCTAC TGGAAATGGG ACACCCAGAC AGTGAGCAAA TCAGCCGCCG ACAGTCCCAA 
GTGGATCGCC TCTATGTGGC GCTCAAGGAG CTGGGCGAGG AACGCAGGGT GAGCCTGGAA 
CAGCAGTACT GGCTCTACCA GCTCAGCCGC CAGGTGGATG AGCTGGAACA CTGGATAGCC 
GAGAAGGAGG TGGTAGCTGG CTCCCCAGAG CTGGGCCAGG ACTTCGAACA CGTGTCGGTG 
CTACAGGAGA AATTCTCAGA GTTTGCCAGT GAGACAGGAA CCGCAGGGCG GGAGCGGCTG 
GCGGCGGTCA ACCAGATGGT GGACGAGCTG ATTGAGTGTG GTCACACAGC AGCGGCCACC 
ATGGCTGAGT GGAAGGACGG GCTGAACGAG GCCTGGGCTG AGCTGCTGGA ACTCATGGGC 
ACTCGGGCCC AGCTGCTCGC TGCCTCTCGG GAGCTGCATA AGTTCTTCAG CGATGCCCGG 
GAGCTTCAAG GGCAGATTGA GGAGAAGCGG AGGAGGCTGC CCCGCCTGAC GGCACCACCT 
GAACCCAGAC CCAGTGCCAG CTCCATGCAG CGGACCCTGC GAGCCTTTGA GCACGACCTG 
CAGCTCCTCG TGTCCCAGGT ACGGCAGCTG CAGGAAGGGG CGGCCCAGCT GCGGACGGTG 
TACGCTGGCG AGCACGCCGA GGCCATCGCC AGCCGCGAGC AGGAGGTGTT GCAGGGCTGG 
AAGGAGCTGC TAGCAGCCTG TGAAGATGCT CGTCTGCACG TCAGCTCCAC GGCCGACGCC 
CTGCGCTTCC ACAGCCAGGC CCGCGACCTG CTCTCCTGGA TGGACGGCAT CGCGGGCCAG 
ATCGGGGCAG CTGACAAACC CAGGGATGTG TCATCGGTGG AGGTGCTTAT GAACTACCAC 
CAGGGCCTGA AGACAGAGTT GGAGGCGCGC GTGCCTGAGC TGGCAACCTG CCAGGAGCTG 
GGGCGGTCTC TATTACTCAA CAAAAGCGCC ATGGCTGATG AGATCCAGGC CCAGCTGGAC 
AAGCTGGGAA GCCGGAAGGA GGAGGTGTCG GAGAAGTGGG ACCGTCACTG GGAGTGGCTG 
CAGCAGATGC TGGAGGTACA CCAGTTTGCT CAGGAGGCCG TGGTGGCTGA CGCCTGGCTG 
ACAGCTCAGG AACCGCTCCT GCAGAGCCGG GAGCTGGGCA GCAGTGTGGA TGAGGTGGAG 
CAGCTTATCC GGCGACACGA GGCCTTCCGA AAAGCAGCTG CGGCCTGGGA GGAGAGGTTC 
AGCTCTCTGC GGCGCTTGAC CACGATCGAG AAACTCAAGG CAGAACAGAG TAAGCAGCCA 
CCCACCCCCT TACTGGGGCG CAAGTTCTTT GGGGACCCCA CAGAGCTGGC GGCCAAGGCA 
GCGCCTTTGC TGCGTCCAGG GGGCTATGAC AGGGGCCTGG AGCCTTTGGC CCGCAGGGCC 
TCGGACACGC TGTCAGCGGA GGTGCGGACC CGGGTTGGGT ATGTGCGCCA GGAGCTCAAG 
CCCGAGCGTC TCCAGCCGCG CATTGACCGG CTGCCAGAGA CCTCGGGGAA GGTAGAACCC 
GCGGCCCCGA CAGCCGCAGC GTTGGACACG ACGGACACCC CTGGGACTCC GGCGGCGACG 
GAGTTGGTCC GGCCCCGGTC GGAGCGCCAG GAGTTAGCGG ATCGTGCGGA GGAGCTGCCA 
CGGAGACGAA GATCAGAACG CCAGGAGTCC GTGGATCAAC CAGAGGAGAC AGCGCGGAGG 
CGGCGGCCTG AGCGCCAGGA GTCCGCGGAC CACGAGGGGC CGCACAGCCT TACTCTAGGT 
CGCTACGAGC AGATGGAGAG ACGGCGGGAG CGGAGGGAGC GGCGGATAGA ACGACAGGAG 
TCTAGCGAAC AGGAGACTCC CACCAGGGGA GAGCTGGTCA AAGGGAAGGC CACTCTGGCT 
GATATTGTGG AGCAACTTCA GGAGAAAGAG GCAGGCCCAG GGATCCCTGC TGGGGTGCCG 
TCGCTGCCTC AGCCTCGCGA GCTTCCCCCT GGTCGCCTGC CCAACGGGCT TGAGCCGCCC 
GAGCGGACAC CTCGGCCCGA TCGGCCGCGG GCACGGGACC GGCCCAAGCC GCGCCGGCGG 
CCACGGCCTC GAGAGGGTGG TGAGGGCGGG GGAAGCCGGC GCTCGCGCTC CGCCCCTGCC 
CAGGGAGGCT CCGCCCCCGC GCCGCCACCT CCGCCCACTC ACACAGTGCA GCACGAGGGC 
TTCCTGCTGC GCAAGCGCGA GCTCGACGCT AACCGCAAGT CGTCCAACCG GTCATGGGTG 
AGCCTGTACT GTGTGCTCAG CAAGGGGGAG CTGGGCTTCT ACAAGGACTC CAAAGGCCCA 
GCATCAGGGG GCACGCATGG TGGGGAACCA CTGCTCAGCC TGCACAAAGC CACCAGCGAG 
GTGGCTAGTG ACTACAAGAA AAAGAAGCAT GTCTTCAAGC TCCAGACCCA GGATGGCAGT 
GAGTTCTTGC TCCAGGCTAA AGATGAGGAG GAGATGAACG GCTGGCTGGA GGCTGTGGCT 
AACTCCGTGG CAGAACACGC CGAGATCGCC CGATGGGGTC AGACACTACC TACCACATCA 
TCCACAGATG AGGGCAACCC CAAGCGTGAG GGCGGGGAGC GTAGGGCCAG CGGGCGCCGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186164.1
CDS	227..3952
Translation	MLMARDGTREDSHKLHKRWLRHQAFMAELAQNKEWLEKIEREGQQLMQEKPELAASVRKKLGEIRQCWAELESTTQAKARQLFEASKADQLVQSFAELDKRLLHMESQLQDVDPGGDLATVNSQLKKLQSMESQVEEWCREVGELQAQTAALPLEQASKELVGERQSAVGERLVRLLEPLQERRRLLLASKELHQVAHDLDDELAWVQERLPLAMQTERGTGLQAVQQHIKKNQGLRREIQAHGPRLEEVLERAGVLASLRSPEAEAVRRGQEQLQSAWTGLREAAERRQQTLDAAFQVEQYYFDVAEVEAWLGEQELLMMSEDKGKDEQSTLQLLKKHLQLEQGVENYEESIAQLSRQCRALLEMGHPDSEQISRRQSQVDRLYVALKELGEERRVSLEQQYWLYQLSRQVDELEHWIAEKEVVAGSPELGQDFEHVSVLQEKFSEFASETGTAGRERLAAVNQMVDELIECGHTAAATMAEWKDGLNEAWAELLELMGTRAQLLAASRELHKFFSDARELQGQIEEKRRRLPRLTAPPEPRPSASSMQRTLRAFEHDLQLLVSQVRQLQEGAAQLRTVYAGEHAEAIASREQEVLQGWKELLAACEDARLHVSSTADALRFHSQARDLLSWMDGIAGQIGAADKPRDVSSVEVLMNYHQGLKTELEARVPELATCQELGRSLLLNKSAMADEIQAQLDKLGSRKEEVSEKWDRHWEWLQQMLEVHQFAQEAVVADAWLTAQEPLLQSRELGSSVDEVEQLIRRHEAFRKAAAAWEERFSSLRRLTTIEKLKAEQSKQPPTPLLGRKFFGDPTELAAKAAPLLRPGGYDRGLEPLARRASDTLSAEVRTRVGYVRQELKPERLQPRIDRLPETSGKVEPAAPTAAALDTTDTPGTPAATELVRPRSERQELADRAEELPRRRRSERQESVDQPEETARRRRPERQESADHEGPHSLTLGRYEQMERRRERRERRIERQESSEQETPTRGELVKGKATLADIVEQLQEKEAGPGIPAGVPSLPQPRELPPGRLPNGLEPPERTPRPDRPRARDRPKPRRRPRPREGGEGGGSRRSRSAPAQGGSAPAPPPPPTHTVQHEGFLLRKRELDANRKSSNRSWVSLYCVLSKGELGFYKDSKGPASGGTHGGEPLLSLHKATSEVASDYKKKKHVFKLQTQDGSEFLLQAKDEEEMNGWLEAVANSVAEHAEIARWGQTLPTTSSTDEGNPKREGGERRASGRRK

Target ORF information:

RefSeq Version	NM_001199235.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant sigma6b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199235.1

ORF Insert Sequence:

ATGCTGATGG CTCGTGATGG CACCCGGGAA GACAGCCACA AGCTGCACAA GAGATGGCTC 
CGGCACCAGG CGTTCATGGC TGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATAGAA 
AGGGAGGGCC AGCAACTGAT GCAGGAGAAA CCGGAGCTGG CAGCCTCTGT GCGCAAGAAA 
TTAGGTGAGA TCCGGCAGTG CTGGGCGGAG CTGGAGAGCA CCACACAGGC CAAGGCGCGC 
CAGCTCTTCG AGGCCAGCAA GGCGGACCAG CTAGTGCAGA GCTTCGCGGA GCTGGACAAG 
CGGCTGCTTC ACATGGAAAG CCAGCTGCAA GACGTAGACC CCGGTGGGGA CCTGGCCACT 
GTCAACAGCC AGCTCAAGAA GTTACAGTCC ATGGAGTCTC AGGTGGAGGA GTGGTGCCGA 
GAGGTGGGCG AGCTTCAGGC GCAGACGGCG GCGCTTCCGC TGGAGCAGGC GAGCAAGGAG 
TTGGTGGGGG AGCGGCAGAG TGCGGTGGGC GAGCGCCTGG TGCGCCTGCT GGAGCCTTTG 
CAGGAGCGAC GCCGCTTGTT GCTGGCATCC AAGGAGCTGC ATCAGGTGGC CCACGACCTG 
GACGATGAAC TGGCTTGGGT TCAAGAGCGG CTGCCACTGG CCATGCAGAC AGAGCGAGGC 
ACTGGCTTGC AGGCTGTCCA GCAGCACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC 
CAGGCTCACG GGCCGCGCTT GGAGGAGGTG CTGGAGCGCG CGGGCGTGCT GGCCTCGCTG 
CGCAGCCCAG AGGCCGAAGC GGTGCGCCGT GGCCAAGAGC AGCTGCAGAG CGCCTGGACC 
GGGCTGCGGG AAGCGGCCGA ACGGCGGCAG CAGACGCTGG ACGCCGCCTT CCAGGTGGAA 
CAGTACTACT TTGACGTGGC TGAGGTGGAG GCATGGCTGG GCGAGCAGGA GCTGCTCATG 
ATGAGTGAGG ACAAGGGCAA GGATGAACAG AGCACCCTGC AGCTGCTCAA GAAGCATTTA 
CAACTGGAAC AGGGCGTGGA GAACTATGAG GAAAGCATCG CCCAGCTGTC ACGCCAGTGC 
CGGGCGCTAC TGGAAATGGG ACACCCAGAC AGTGAGCAAA TCAGCCGCCG ACAGTCCCAA 
GTGGATCGCC TCTATGTGGC GCTCAAGGAG CTGGGCGAGG AACGCAGGGT GAGCCTGGAA 
CAGCAGTACT GGCTCTACCA GCTCAGCCGC CAGGTGGATG AGCTGGAACA CTGGATAGCC 
GAGAAGGAGG TGGTAGCTGG CTCCCCAGAG CTGGGCCAGG ACTTCGAACA CGTGTCGGTG 
CTACAGGAGA AATTCTCAGA GTTTGCCAGT GAGACAGGAA CCGCAGGGCG GGAGCGGCTG 
GCGGCGGTCA ACCAGATGGT GGACGAGCTG ATTGAGTGTG GTCACACAGC AGCGGCCACC 
ATGGCTGAGT GGAAGGACGG GCTGAACGAG GCCTGGGCTG AGCTGCTGGA ACTCATGGGC 
ACTCGGGCCC AGCTGCTCGC TGCCTCTCGG GAGCTGCATA AGTTCTTCAG CGATGCCCGG 
GAGCTTCAAG GGCAGATTGA GGAGAAGCGG AGGAGGCTGC CCCGCCTGAC GGCACCACCT 
GAACCCAGAC CCAGTGCCAG CTCCATGCAG CGGACCCTGC GAGCCTTTGA GCACGACCTG 
CAGCTCCTCG TGTCCCAGGT ACGGCAGCTG CAGGAAGGGG CGGCCCAGCT GCGGACGGTG 
TACGCTGGCG AGCACGCCGA GGCCATCGCC AGCCGCGAGC AGGAGGTGTT GCAGGGCTGG 
AAGGAGCTGC TAGCAGCCTG TGAAGATGCT CGTCTGCACG TCAGCTCCAC GGCCGACGCC 
CTGCGCTTCC ACAGCCAGGC CCGCGACCTG CTCTCCTGGA TGGACGGCAT CGCGGGCCAG 
ATCGGGGCAG CTGACAAACC CAGGGATGTG TCATCGGTGG AGGTGCTTAT GAACTACCAC 
CAGGGCCTGA AGACAGAGTT GGAGGCGCGC GTGCCTGAGC TGGCAACCTG CCAGGAGCTG 
GGGCGGTCTC TATTACTCAA CAAAAGCGCC ATGGCTGATG AGATCCAGGC CCAGCTGGAC 
AAGCTGGGAA GCCGGAAGGA GGAGGTGTCG GAGAAGTGGG ACCGTCACTG GGAGTGGCTG 
CAGCAGATGC TGGAGGTACA CCAGTTTGCT CAGGAGGCCG TGGTGGCTGA CGCCTGGCTG 
ACAGCTCAGG AACCGCTCCT GCAGAGCCGG GAGCTGGGCA GCAGTGTGGA TGAGGTGGAG 
CAGCTTATCC GGCGACACGA GGCCTTCCGA AAAGCAGCTG CGGCCTGGGA GGAGAGGTTC 
AGCTCTCTGC GGCGCTTGAC CACGATCGAG AAACTCAAGG CAGAACAGAG TAAGCAGCCA 
CCCACCCCCT TACTGGGGCG CAAGTTCTTT GGGGACCCCA CAGAGCTGGC GGCCAAGGCA 
GCGCCTTTGC TGCGTCCAGG GGGCTATGAC AGGGGCCTGG AGCCTTTGGC CCGCAGGGCC 
TCGGACACGC TGTCAGCGGA GGTGCGGACC CGGGTTGGGT ATGTGCGCCA GGAGCTCAAG 
CCCGAGCGTC TCCAGCCGCG CATTGACCGG CTGCCAGAGA CCTCGGGGAA GGTAGAACCC 
GCGGCCCCGA CAGCCGCAGC GTTGGACACG ACGGACACCC CTGGGACTCC GGCGGCGACG 
GAGTTGGTCC GGCCCCGGTC GGAGCGCCAG GAGTTAGCGG ATCGTGCGGA GGAGCTGCCA 
CGGAGACGAA GATCAGAACG CCAGGAGTCC GTGGATCAAC CAGAGGAGAC AGCGCGGAGG 
CGGCGGCCTG AGCGCCAGGA GTCCGCGGAC CACGAGGGGC CGCACAGCCT TACTCTAGGT 
CGCTACGAGC AGATGGAGAG ACGGCGGGAG CGGAGGGAGC GGCGGATAGA ACGACAGGAG 
TCTAGCGAAC AGGAGACTCC CACCAGGGGA GAGCTGGTCA AAGGGAAGGC CACTCTGGCT 
GATATTGTGG AGCAACTTCA GGAGAAAGAG GCAGGCCCAG GGATCCCTGC TGGGGTGCCG 
TCGCTGCCTC AGCCTCGCGA GCTTCCCCCT GGTCGCCTGC CCAACGGGCT TGAGCCGCCC 
GAGCGGACAC CTCGGCCCGA TCGGCCGCGG GCACGGGACC GGCCCAAGCC GCGCCGGCGG 
CCACGGCCTC GAGAGGGTGG TGAGGGCGGG GGAAGCCGGC GCTCGCGCTC CGCCCCTGCC 
CAGGGAGGCT CCGCCCCCGC GCCGCCACCT CCGCCCACTC ACACAGTGCA GCACGAGGGC 
TTCCTGCTGC GCAAGCGCGA GCTCGACGCT AACCGCAAGT CGTCCAACCG GTCATGGGTG 
AGCCTGTACT GTGTGCTCAG CAAGGGGGAG CTGGGCTTCT ACAAGGACTC CAAAGGCCCA 
GCATCAGGGG GCACGCATGG TGGGGAACCA CTGCTCAGCC TGCACAAAGC CACCAGCGAG 
GTGGCTAGTG ACTACAAGAA AAAGAAGCAT GTCTTCAAGC TCCAGACCCA GGATGGCAGT 
GAGTTCTTGC TCCAGGCTAA AGATGAGGAG GAGATGAACG GCTGGCTGGA GGCTGTGGCT 
AACTCCGTGG CAGAACACGC CGAGATCGCC CGATGGGGTC AGACACTACC TACCACATCA 
TCCACAGATG AGGGCAACCC CAAGCGTGAG GGCGGGGAGC GTAGGGCCAG CGGGCGCCGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn4 ( NM_001199236.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001186165.1 Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant sigma6c, mRNA.

Price & Availability↑

CloneID	OMu02126
Clone ID Related Accession (Same CDS sequence)	NM_001199234.1 , NM_001199235.1 , NM_001199236.1
Accession Version	NM_001199236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 4 isoform sigma6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC157561.10. Transcript Variant: This variant (sigma6c) has a shorter and distinct 5' terminus which results in the use of an in-frame downstream translation initiation signal, compared to sigma1. Compared to isoform sigma1, it encodes an isoform (sigma6) that lacks multiple calponin-homology domains and spectrin repeats at the N-terminus. Variants sigma6a-c encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments to AB055621.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB055621.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGCTGATGG CTCGTGATGG CACCCGGGAA GACAGCCACA AGCTGCACAA GAGATGGCTC 
CGGCACCAGG CGTTCATGGC TGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATAGAA 
AGGGAGGGCC AGCAACTGAT GCAGGAGAAA CCGGAGCTGG CAGCCTCTGT GCGCAAGAAA 
TTAGGTGAGA TCCGGCAGTG CTGGGCGGAG CTGGAGAGCA CCACACAGGC CAAGGCGCGC 
CAGCTCTTCG AGGCCAGCAA GGCGGACCAG CTAGTGCAGA GCTTCGCGGA GCTGGACAAG 
CGGCTGCTTC ACATGGAAAG CCAGCTGCAA GACGTAGACC CCGGTGGGGA CCTGGCCACT 
GTCAACAGCC AGCTCAAGAA GTTACAGTCC ATGGAGTCTC AGGTGGAGGA GTGGTGCCGA 
GAGGTGGGCG AGCTTCAGGC GCAGACGGCG GCGCTTCCGC TGGAGCAGGC GAGCAAGGAG 
TTGGTGGGGG AGCGGCAGAG TGCGGTGGGC GAGCGCCTGG TGCGCCTGCT GGAGCCTTTG 
CAGGAGCGAC GCCGCTTGTT GCTGGCATCC AAGGAGCTGC ATCAGGTGGC CCACGACCTG 
GACGATGAAC TGGCTTGGGT TCAAGAGCGG CTGCCACTGG CCATGCAGAC AGAGCGAGGC 
ACTGGCTTGC AGGCTGTCCA GCAGCACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC 
CAGGCTCACG GGCCGCGCTT GGAGGAGGTG CTGGAGCGCG CGGGCGTGCT GGCCTCGCTG 
CGCAGCCCAG AGGCCGAAGC GGTGCGCCGT GGCCAAGAGC AGCTGCAGAG CGCCTGGACC 
GGGCTGCGGG AAGCGGCCGA ACGGCGGCAG CAGACGCTGG ACGCCGCCTT CCAGGTGGAA 
CAGTACTACT TTGACGTGGC TGAGGTGGAG GCATGGCTGG GCGAGCAGGA GCTGCTCATG 
ATGAGTGAGG ACAAGGGCAA GGATGAACAG AGCACCCTGC AGCTGCTCAA GAAGCATTTA 
CAACTGGAAC AGGGCGTGGA GAACTATGAG GAAAGCATCG CCCAGCTGTC ACGCCAGTGC 
CGGGCGCTAC TGGAAATGGG ACACCCAGAC AGTGAGCAAA TCAGCCGCCG ACAGTCCCAA 
GTGGATCGCC TCTATGTGGC GCTCAAGGAG CTGGGCGAGG AACGCAGGGT GAGCCTGGAA 
CAGCAGTACT GGCTCTACCA GCTCAGCCGC CAGGTGGATG AGCTGGAACA CTGGATAGCC 
GAGAAGGAGG TGGTAGCTGG CTCCCCAGAG CTGGGCCAGG ACTTCGAACA CGTGTCGGTG 
CTACAGGAGA AATTCTCAGA GTTTGCCAGT GAGACAGGAA CCGCAGGGCG GGAGCGGCTG 
GCGGCGGTCA ACCAGATGGT GGACGAGCTG ATTGAGTGTG GTCACACAGC AGCGGCCACC 
ATGGCTGAGT GGAAGGACGG GCTGAACGAG GCCTGGGCTG AGCTGCTGGA ACTCATGGGC 
ACTCGGGCCC AGCTGCTCGC TGCCTCTCGG GAGCTGCATA AGTTCTTCAG CGATGCCCGG 
GAGCTTCAAG GGCAGATTGA GGAGAAGCGG AGGAGGCTGC CCCGCCTGAC GGCACCACCT 
GAACCCAGAC CCAGTGCCAG CTCCATGCAG CGGACCCTGC GAGCCTTTGA GCACGACCTG 
CAGCTCCTCG TGTCCCAGGT ACGGCAGCTG CAGGAAGGGG CGGCCCAGCT GCGGACGGTG 
TACGCTGGCG AGCACGCCGA GGCCATCGCC AGCCGCGAGC AGGAGGTGTT GCAGGGCTGG 
AAGGAGCTGC TAGCAGCCTG TGAAGATGCT CGTCTGCACG TCAGCTCCAC GGCCGACGCC 
CTGCGCTTCC ACAGCCAGGC CCGCGACCTG CTCTCCTGGA TGGACGGCAT CGCGGGCCAG 
ATCGGGGCAG CTGACAAACC CAGGGATGTG TCATCGGTGG AGGTGCTTAT GAACTACCAC 
CAGGGCCTGA AGACAGAGTT GGAGGCGCGC GTGCCTGAGC TGGCAACCTG CCAGGAGCTG 
GGGCGGTCTC TATTACTCAA CAAAAGCGCC ATGGCTGATG AGATCCAGGC CCAGCTGGAC 
AAGCTGGGAA GCCGGAAGGA GGAGGTGTCG GAGAAGTGGG ACCGTCACTG GGAGTGGCTG 
CAGCAGATGC TGGAGGTACA CCAGTTTGCT CAGGAGGCCG TGGTGGCTGA CGCCTGGCTG 
ACAGCTCAGG AACCGCTCCT GCAGAGCCGG GAGCTGGGCA GCAGTGTGGA TGAGGTGGAG 
CAGCTTATCC GGCGACACGA GGCCTTCCGA AAAGCAGCTG CGGCCTGGGA GGAGAGGTTC 
AGCTCTCTGC GGCGCTTGAC CACGATCGAG AAACTCAAGG CAGAACAGAG TAAGCAGCCA 
CCCACCCCCT TACTGGGGCG CAAGTTCTTT GGGGACCCCA CAGAGCTGGC GGCCAAGGCA 
GCGCCTTTGC TGCGTCCAGG GGGCTATGAC AGGGGCCTGG AGCCTTTGGC CCGCAGGGCC 
TCGGACACGC TGTCAGCGGA GGTGCGGACC CGGGTTGGGT ATGTGCGCCA GGAGCTCAAG 
CCCGAGCGTC TCCAGCCGCG CATTGACCGG CTGCCAGAGA CCTCGGGGAA GGTAGAACCC 
GCGGCCCCGA CAGCCGCAGC GTTGGACACG ACGGACACCC CTGGGACTCC GGCGGCGACG 
GAGTTGGTCC GGCCCCGGTC GGAGCGCCAG GAGTTAGCGG ATCGTGCGGA GGAGCTGCCA 
CGGAGACGAA GATCAGAACG CCAGGAGTCC GTGGATCAAC CAGAGGAGAC AGCGCGGAGG 
CGGCGGCCTG AGCGCCAGGA GTCCGCGGAC CACGAGGGGC CGCACAGCCT TACTCTAGGT 
CGCTACGAGC AGATGGAGAG ACGGCGGGAG CGGAGGGAGC GGCGGATAGA ACGACAGGAG 
TCTAGCGAAC AGGAGACTCC CACCAGGGGA GAGCTGGTCA AAGGGAAGGC CACTCTGGCT 
GATATTGTGG AGCAACTTCA GGAGAAAGAG GCAGGCCCAG GGATCCCTGC TGGGGTGCCG 
TCGCTGCCTC AGCCTCGCGA GCTTCCCCCT GGTCGCCTGC CCAACGGGCT TGAGCCGCCC 
GAGCGGACAC CTCGGCCCGA TCGGCCGCGG GCACGGGACC GGCCCAAGCC GCGCCGGCGG 
CCACGGCCTC GAGAGGGTGG TGAGGGCGGG GGAAGCCGGC GCTCGCGCTC CGCCCCTGCC 
CAGGGAGGCT CCGCCCCCGC GCCGCCACCT CCGCCCACTC ACACAGTGCA GCACGAGGGC 
TTCCTGCTGC GCAAGCGCGA GCTCGACGCT AACCGCAAGT CGTCCAACCG GTCATGGGTG 
AGCCTGTACT GTGTGCTCAG CAAGGGGGAG CTGGGCTTCT ACAAGGACTC CAAAGGCCCA 
GCATCAGGGG GCACGCATGG TGGGGAACCA CTGCTCAGCC TGCACAAAGC CACCAGCGAG 
GTGGCTAGTG ACTACAAGAA AAAGAAGCAT GTCTTCAAGC TCCAGACCCA GGATGGCAGT 
GAGTTCTTGC TCCAGGCTAA AGATGAGGAG GAGATGAACG GCTGGCTGGA GGCTGTGGCT 
AACTCCGTGG CAGAACACGC CGAGATCGCC CGATGGGGTC AGACACTACC TACCACATCA 
TCCACAGATG AGGGCAACCC CAAGCGTGAG GGCGGGGAGC GTAGGGCCAG CGGGCGCCGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186165.1
CDS	90..3815
Translation	MLMARDGTREDSHKLHKRWLRHQAFMAELAQNKEWLEKIEREGQQLMQEKPELAASVRKKLGEIRQCWAELESTTQAKARQLFEASKADQLVQSFAELDKRLLHMESQLQDVDPGGDLATVNSQLKKLQSMESQVEEWCREVGELQAQTAALPLEQASKELVGERQSAVGERLVRLLEPLQERRRLLLASKELHQVAHDLDDELAWVQERLPLAMQTERGTGLQAVQQHIKKNQGLRREIQAHGPRLEEVLERAGVLASLRSPEAEAVRRGQEQLQSAWTGLREAAERRQQTLDAAFQVEQYYFDVAEVEAWLGEQELLMMSEDKGKDEQSTLQLLKKHLQLEQGVENYEESIAQLSRQCRALLEMGHPDSEQISRRQSQVDRLYVALKELGEERRVSLEQQYWLYQLSRQVDELEHWIAEKEVVAGSPELGQDFEHVSVLQEKFSEFASETGTAGRERLAAVNQMVDELIECGHTAAATMAEWKDGLNEAWAELLELMGTRAQLLAASRELHKFFSDARELQGQIEEKRRRLPRLTAPPEPRPSASSMQRTLRAFEHDLQLLVSQVRQLQEGAAQLRTVYAGEHAEAIASREQEVLQGWKELLAACEDARLHVSSTADALRFHSQARDLLSWMDGIAGQIGAADKPRDVSSVEVLMNYHQGLKTELEARVPELATCQELGRSLLLNKSAMADEIQAQLDKLGSRKEEVSEKWDRHWEWLQQMLEVHQFAQEAVVADAWLTAQEPLLQSRELGSSVDEVEQLIRRHEAFRKAAAAWEERFSSLRRLTTIEKLKAEQSKQPPTPLLGRKFFGDPTELAAKAAPLLRPGGYDRGLEPLARRASDTLSAEVRTRVGYVRQELKPERLQPRIDRLPETSGKVEPAAPTAAALDTTDTPGTPAATELVRPRSERQELADRAEELPRRRRSERQESVDQPEETARRRRPERQESADHEGPHSLTLGRYEQMERRRERRERRIERQESSEQETPTRGELVKGKATLADIVEQLQEKEAGPGIPAGVPSLPQPRELPPGRLPNGLEPPERTPRPDRPRARDRPKPRRRPRPREGGEGGGSRRSRSAPAQGGSAPAPPPPPTHTVQHEGFLLRKRELDANRKSSNRSWVSLYCVLSKGELGFYKDSKGPASGGTHGGEPLLSLHKATSEVASDYKKKKHVFKLQTQDGSEFLLQAKDEEEMNGWLEAVANSVAEHAEIARWGQTLPTTSSTDEGNPKREGGERRASGRRK

Target ORF information:

RefSeq Version	NM_001199236.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant sigma6c, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199236.1

ORF Insert Sequence:

ATGCTGATGG CTCGTGATGG CACCCGGGAA GACAGCCACA AGCTGCACAA GAGATGGCTC 
CGGCACCAGG CGTTCATGGC TGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATAGAA 
AGGGAGGGCC AGCAACTGAT GCAGGAGAAA CCGGAGCTGG CAGCCTCTGT GCGCAAGAAA 
TTAGGTGAGA TCCGGCAGTG CTGGGCGGAG CTGGAGAGCA CCACACAGGC CAAGGCGCGC 
CAGCTCTTCG AGGCCAGCAA GGCGGACCAG CTAGTGCAGA GCTTCGCGGA GCTGGACAAG 
CGGCTGCTTC ACATGGAAAG CCAGCTGCAA GACGTAGACC CCGGTGGGGA CCTGGCCACT 
GTCAACAGCC AGCTCAAGAA GTTACAGTCC ATGGAGTCTC AGGTGGAGGA GTGGTGCCGA 
GAGGTGGGCG AGCTTCAGGC GCAGACGGCG GCGCTTCCGC TGGAGCAGGC GAGCAAGGAG 
TTGGTGGGGG AGCGGCAGAG TGCGGTGGGC GAGCGCCTGG TGCGCCTGCT GGAGCCTTTG 
CAGGAGCGAC GCCGCTTGTT GCTGGCATCC AAGGAGCTGC ATCAGGTGGC CCACGACCTG 
GACGATGAAC TGGCTTGGGT TCAAGAGCGG CTGCCACTGG CCATGCAGAC AGAGCGAGGC 
ACTGGCTTGC AGGCTGTCCA GCAGCACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC 
CAGGCTCACG GGCCGCGCTT GGAGGAGGTG CTGGAGCGCG CGGGCGTGCT GGCCTCGCTG 
CGCAGCCCAG AGGCCGAAGC GGTGCGCCGT GGCCAAGAGC AGCTGCAGAG CGCCTGGACC 
GGGCTGCGGG AAGCGGCCGA ACGGCGGCAG CAGACGCTGG ACGCCGCCTT CCAGGTGGAA 
CAGTACTACT TTGACGTGGC TGAGGTGGAG GCATGGCTGG GCGAGCAGGA GCTGCTCATG 
ATGAGTGAGG ACAAGGGCAA GGATGAACAG AGCACCCTGC AGCTGCTCAA GAAGCATTTA 
CAACTGGAAC AGGGCGTGGA GAACTATGAG GAAAGCATCG CCCAGCTGTC ACGCCAGTGC 
CGGGCGCTAC TGGAAATGGG ACACCCAGAC AGTGAGCAAA TCAGCCGCCG ACAGTCCCAA 
GTGGATCGCC TCTATGTGGC GCTCAAGGAG CTGGGCGAGG AACGCAGGGT GAGCCTGGAA 
CAGCAGTACT GGCTCTACCA GCTCAGCCGC CAGGTGGATG AGCTGGAACA CTGGATAGCC 
GAGAAGGAGG TGGTAGCTGG CTCCCCAGAG CTGGGCCAGG ACTTCGAACA CGTGTCGGTG 
CTACAGGAGA AATTCTCAGA GTTTGCCAGT GAGACAGGAA CCGCAGGGCG GGAGCGGCTG 
GCGGCGGTCA ACCAGATGGT GGACGAGCTG ATTGAGTGTG GTCACACAGC AGCGGCCACC 
ATGGCTGAGT GGAAGGACGG GCTGAACGAG GCCTGGGCTG AGCTGCTGGA ACTCATGGGC 
ACTCGGGCCC AGCTGCTCGC TGCCTCTCGG GAGCTGCATA AGTTCTTCAG CGATGCCCGG 
GAGCTTCAAG GGCAGATTGA GGAGAAGCGG AGGAGGCTGC CCCGCCTGAC GGCACCACCT 
GAACCCAGAC CCAGTGCCAG CTCCATGCAG CGGACCCTGC GAGCCTTTGA GCACGACCTG 
CAGCTCCTCG TGTCCCAGGT ACGGCAGCTG CAGGAAGGGG CGGCCCAGCT GCGGACGGTG 
TACGCTGGCG AGCACGCCGA GGCCATCGCC AGCCGCGAGC AGGAGGTGTT GCAGGGCTGG 
AAGGAGCTGC TAGCAGCCTG TGAAGATGCT CGTCTGCACG TCAGCTCCAC GGCCGACGCC 
CTGCGCTTCC ACAGCCAGGC CCGCGACCTG CTCTCCTGGA TGGACGGCAT CGCGGGCCAG 
ATCGGGGCAG CTGACAAACC CAGGGATGTG TCATCGGTGG AGGTGCTTAT GAACTACCAC 
CAGGGCCTGA AGACAGAGTT GGAGGCGCGC GTGCCTGAGC TGGCAACCTG CCAGGAGCTG 
GGGCGGTCTC TATTACTCAA CAAAAGCGCC ATGGCTGATG AGATCCAGGC CCAGCTGGAC 
AAGCTGGGAA GCCGGAAGGA GGAGGTGTCG GAGAAGTGGG ACCGTCACTG GGAGTGGCTG 
CAGCAGATGC TGGAGGTACA CCAGTTTGCT CAGGAGGCCG TGGTGGCTGA CGCCTGGCTG 
ACAGCTCAGG AACCGCTCCT GCAGAGCCGG GAGCTGGGCA GCAGTGTGGA TGAGGTGGAG 
CAGCTTATCC GGCGACACGA GGCCTTCCGA AAAGCAGCTG CGGCCTGGGA GGAGAGGTTC 
AGCTCTCTGC GGCGCTTGAC CACGATCGAG AAACTCAAGG CAGAACAGAG TAAGCAGCCA 
CCCACCCCCT TACTGGGGCG CAAGTTCTTT GGGGACCCCA CAGAGCTGGC GGCCAAGGCA 
GCGCCTTTGC TGCGTCCAGG GGGCTATGAC AGGGGCCTGG AGCCTTTGGC CCGCAGGGCC 
TCGGACACGC TGTCAGCGGA GGTGCGGACC CGGGTTGGGT ATGTGCGCCA GGAGCTCAAG 
CCCGAGCGTC TCCAGCCGCG CATTGACCGG CTGCCAGAGA CCTCGGGGAA GGTAGAACCC 
GCGGCCCCGA CAGCCGCAGC GTTGGACACG ACGGACACCC CTGGGACTCC GGCGGCGACG 
GAGTTGGTCC GGCCCCGGTC GGAGCGCCAG GAGTTAGCGG ATCGTGCGGA GGAGCTGCCA 
CGGAGACGAA GATCAGAACG CCAGGAGTCC GTGGATCAAC CAGAGGAGAC AGCGCGGAGG 
CGGCGGCCTG AGCGCCAGGA GTCCGCGGAC CACGAGGGGC CGCACAGCCT TACTCTAGGT 
CGCTACGAGC AGATGGAGAG ACGGCGGGAG CGGAGGGAGC GGCGGATAGA ACGACAGGAG 
TCTAGCGAAC AGGAGACTCC CACCAGGGGA GAGCTGGTCA AAGGGAAGGC CACTCTGGCT 
GATATTGTGG AGCAACTTCA GGAGAAAGAG GCAGGCCCAG GGATCCCTGC TGGGGTGCCG 
TCGCTGCCTC AGCCTCGCGA GCTTCCCCCT GGTCGCCTGC CCAACGGGCT TGAGCCGCCC 
GAGCGGACAC CTCGGCCCGA TCGGCCGCGG GCACGGGACC GGCCCAAGCC GCGCCGGCGG 
CCACGGCCTC GAGAGGGTGG TGAGGGCGGG GGAAGCCGGC GCTCGCGCTC CGCCCCTGCC 
CAGGGAGGCT CCGCCCCCGC GCCGCCACCT CCGCCCACTC ACACAGTGCA GCACGAGGGC 
TTCCTGCTGC GCAAGCGCGA GCTCGACGCT AACCGCAAGT CGTCCAACCG GTCATGGGTG 
AGCCTGTACT GTGTGCTCAG CAAGGGGGAG CTGGGCTTCT ACAAGGACTC CAAAGGCCCA 
GCATCAGGGG GCACGCATGG TGGGGAACCA CTGCTCAGCC TGCACAAAGC CACCAGCGAG 
GTGGCTAGTG ACTACAAGAA AAAGAAGCAT GTCTTCAAGC TCCAGACCCA GGATGGCAGT 
GAGTTCTTGC TCCAGGCTAA AGATGAGGAG GAGATGAACG GCTGGCTGGA GGCTGTGGCT 
AACTCCGTGG CAGAACACGC CGAGATCGCC CGATGGGGTC AGACACTACC TACCACATCA 
TCCACAGATG AGGGCAACCC CAAGCGTGAG GGCGGGGAGC GTAGGGCCAG CGGGCGCCGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn4 ( NM_032610.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_115999.2 Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant sigma1, mRNA.

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CloneID	OMu20714
Clone ID Related Accession (Same CDS sequence)	NM_032610.2
Accession Version	NM_032610.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 4 isoform sigma1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147558.1, AB055618.1 and AC157561.10. On Oct 14, 2006 this sequence version replaced NM_032610.1. Transcript Variant: This variant (sigma1) represents the longest transcript and encodes the longer isoform (sigma1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB055618.1, AK147558.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACAAG TACCAGGGGA GGTGGACAAC ATGGAGGGCC CAGCAGTCTC CAACAACAAC 
AACCCTTCAG CTCGCTGGGA GAGCCCAGAT CGGGGCTGGG ATCGGGAACC TCCCGCAGCC 
GCCAATGCTG CGGCTTCACT CTTTGAGTGC TCTCGGATCA AGGCTTTAGC AGATGAGCGA 
GAGGCTGTGC AGAAGAAAAC CTTCACCAAG TGGGTGAATT CGCACCTTGC CCGCGTGGGC 
TGCCACATTG GAGACCTGTA TGCAGACCTT CGGGACGGGT TTGTCCTCAC CCGGCTTCTG 
GAAGTGCTGT CGGGGGAGCA GCTGCCGAGG CCCACTCGCG GCCGCATGCG GATCCACTCG 
CTGGAGAACG TGGACAAGGC TCTGCAGTTC CTGAAGGAGC AGCGTGTGCA TCTGGAGAAC 
GTGGGGTCAC ATGACATCGT GGATGGGAAC CACCGACTGA CGCTGGGGCT GGTCTGGACC 
ATCATCCTGC GCTTCCAGAT TCAAGTCATC AAGATTGAGA CAGAAGACAA CAGAGAGACC 
CGCTCCGCCA AGGACGCTCT GCTCTTGTGG TGCCAGATGA AAACAGCCGG TTACCCTGAG 
GTAAACATCC AGAATTTCAC CACCAGCTGG AGAGATGGCC TGGCCTTCAA TGCCCTCATT 
CACCGTCACA GGCCTGATCT TGTGGACCTC AGCAAACTTA CCAAGTCCAA CGCCAACTAC 
AACCTGCAGA GAGCTTTCCG CACAGCCGAG CAGCACCTGG GGCTAGCACG TCTGCTGGAC 
CCCGAAGATG TGAACATGGA GGCTCCAGAT GAGAAGTCCA TTATCACCTA TGTGGTCTCC 
TTCTACCACT ACTTCTCCAA GATGAAGGCT CTGGCGGTGG AGGGAAAACG GATTGGAAAG 
GTCCTGGACC AGGTGTTGGA GGTGGACAAG ATCATAGAAC GCTATGAGGA GCTGGCGGCG 
GAGCTGTTGG CCTGGATCCA TCGCACTGTG GACCTCATTA GCAACCAAAA GTTTGCTAAC 
TCACTGAGTG GGGTGCAGCA GCAGCTGCAG GCCTTCACGG CTTACTGTAC GCTGGAGAAG 
CCCGTCAAGT TTCAGGAGAA GGGGAACCTG GAGGTGCTAC TCTTCAGCAT CCAAAGCAAA 
CTGCGCGCCC ACAACCGGCG CCTCTTTGTG CCTCGAGAAG GCTGTGGCAT CTGGGATATT 
GACAAGGCGT GGGGTGAGCT GGAGAAGGCA GAGCATGAGC GAGAGGCGGC CCTCAGAGCT 
GAGCTCATCC GGCAGGAGAA GTTGGAACTC TTGGCTCAAA GATTTGACCA CAAGGTGGCC 
ATGAGGGAGA GCTGGCTAAA TGAGAACCAG CGCCTGGTCT CCCAGGACAA CTTCGGATAT 
GAGCTGCCGG CAGTGGAGGC AGCCATGAAG AAGCACGAAG CCATTGAGGC AGACATTGCA 
GCCTACGAGG AGCGGGTTCA GGGTGTGGCG GAGTTAGCCC AGGCGTTGGC AGCTGAAGGC 
TACTATGATG CCCGGCGGGT GGCTGCCCAG CGTGACAGTG TCCTTCGCCA GTGGGCCCTG 
CTGACCGGGC TGGTAGGTGC CCGGCGGACC CGGCTTGAGC AGAATCTGGC CCTGCAGAAG 
GTCTTCCAGG AGATGGTCTA CATGGTGGAC TGGATGGAGG AGATGCAGAC TCAGCTGCTG 
TCTCGGGAAT GTGGGCAGCA CCTGGTGGAG GCCGATGACC TGCTGCAGAA GCACGGGCTG 
CTGGAGGGGG ACATCGCAGC CCAGAGTGAG CGAGTGGAGG CCCTCAATGC TGCAGCCCTG 
AGATTCTCCC AGCTACAGGG CTATCAGCCC TGCGACCCTC AGGTCATTTG CAACCGTGTG 
AACCACGTGC ATGGCTGTCT GTCGGAGCTG CAGGAGCAGG CAGCACGGCG GCGCGCAGAG 
CTGGAAGCTT CTCGGAGCCT GTGGGCGCTG CTGCAGGAGC TGGAGGAGGC GGAGAGTTGG 
GCGCGGGACA AGGAGCGCCT CCTAGAGGCC ACGAGCGGCA GTGGTGGCGC GGCGGGCACG 
GCGGGCGGCG CGCACGACCT GTCCAGCACC GCTCGCCTGC TGGCGCAGCA TAAGATCCTG 
CAGGGCGAGC TGGGCGGCCG GCGGGCGCTG CTGCAGCAGG CGCTGCGACG CGGGGAGGAG 
TTGGCTGCGG CGGGCGGCTC CGTGGGCCCG GGCGCCGAGC CTTTGCATTT GGCTGGCCTG 
GCGGAGCGCG CGGCTAGCGC TCGGCGCCGC TGGCAGCGGC TGGAAGAGGC GGCTGCGCGC 
CGGGAACGGC GGCTGCAGGA GGCGCGGGCG CTGCACCAAT TTGGCGCCGA CCTGGACGGG 
CTGCTGGACT GGCTTCGTGA CGCGTATCGT CTAGCAGCCG CTGGAGATTT TGGTCACGAC 
GAAGCGTCCA GCCGTCGCCT GGCGCGCCAG CACCGCGCTC TCACCGGGGA GGTGGAAGCG 
CACCGTGGCC CTGTGGGTGG CCTGAGGCGC CAGCTGGCGA CACTGGGAGG AGCCAGTGGC 
GCAGGGCCGC TGGTGGTGGC TCTGCAGGTG CGCGTGGTGG AAGCCGAACA GTTGTTCGCC 
GAGGTGACCG AGGTGGCCGC GCTGCGACGA CAGTGGCTTC GGGACGCGCT CGCCGTGTAC 
CGCATGTTCG GCGAGGTACA TGCCTGCGAG TTGTGGATCG GGGAGAAAGA GCAGTGGCTG 
CTGGCCATGC GTGTGCCCGA TTCCCTCGAC GACGTTGAGG TGGTGCAGCA TCGATTTGAA 
AGTCTTGACC AGGAAATGAA CAGCCTGATG GGCCGAGTCC TGGATGTGAA CCAAACAGTT 
CAGGAGTTGG TAGAAGGAGG TCATCCCAGC TCCGATGAGG TGCGCTCCTG TCAGGACCAT 
CTCAACAGCA GGTGGAACCG CATCGTGGAG TTGGTGGAAC AGCGCAAAGA GGAGATGAGC 
GCGGTGCTGC TGGTGGAGAA CCACGTGCTG GAGGTGGCCG AGGTGCGCGC CCAAGTGCGC 
GAGAAGCGAC GGGCAGTGGA GAGCGCGCCC CGGGCGGGGG GAGCCCTGCA ATGGCGCCTG 
AGTGGCCTGG AGGCGGCTCT ACAGGCGCTA GAGCCGCGAC AGGCGGCGCT GCTGGAGGAG 
GCGGCGCTGC TGGCCGAACG CTTCCCCGCA CAGGCGACGC GCCTGCACCA GGGCGCCGAG 
GAGCTAGGAG CCGAGTGGGG CGCACTGGCC GGAGCGGCTC AGGCCTGCGG CGAGGCGGTT 
GCGGCGGCAG GTCGCCTGCA GCGTTTCCTG CGCGATTTGG ATACTTTCCT GGACTGGCTG 
GTGCGGGCGC AGGAGGCAGC AGGCGCCGTC GAGGGGCCGC TGCCTCGCAG CCTGGAGGAG 
GCGGATGGGC TGCTGGCGCG CCACGCCGCG CTCAAGGAGG AGGTGGATCA GCGTGAGGAG 
GACTACGCGC GCATCGTGGC TGCCAGTGAA GCGCTGCTAG CGTCCGAGGG CGCGGAGCTG 
GGCCCCGGTC TGGCGCTAGA CGAGTGGCTG CCGCACCTCG AGGTTGGCTG GCACAAACTG 
CTCGGCTTGT GGGAGGAGCG CAGGGAGGCG CTGGTCCAAG CGCACGTCTA TCAACTCTTC 
CTGAGAGACC TGTGCCAGGC GCTTGCGGTG CTGCGCAACC AGGAGGTGGC GCTGTCTGGC 
GCTGAACTCC CTTGCACAGT GGAGTCGGTA GAAGAGGCGA TGAAAAGGCA CCGGGATTTT 
CTCACCACGA TGGAGCTGAA CCAGCAAAAG ATGCAGGTGG CGGTTCAAGC TGCAGAGAGC 
CTGCTGCGGC AAGGCAACGC CTACGGGGAG CAGGCGCAGG AGGCAGTGGC TCGGCTGCTA 
GAGAAGAGCC AAGAAAATCA GCTGAGGGCC CAGCAGTGGA TGCAAAAACT ACTGGACCAG 
CTTGTGCTGC AGCACTTTCT TAGAGATTGT CATGAGCTGG ATGGCTGGAT CCATGAGAAG 
ATGCTGATGG CTCGTGATGG CACCCGGGAA GACAGCCACA AGCTGCACAA GAGATGGCTC 
CGGCACCAGG CGTTCATGGC TGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATAGAA 
AGGGAGGGCC AGCAACTGAT GCAGGAGAAA CCGGAGCTGG CAGCCTCTGT GCGCAAGAAA 
TTAGGTGAGA TCCGGCAGTG CTGGGCGGAG CTGGAGAGCA CCACACAGGC CAAGGCGCGC 
CAGCTCTTCG AGGCCAGCAA GGCGGACCAG CTAGTGCAGA GCTTCGCGGA GCTGGACAAG 
CGGCTGCTTC ACATGGAAAG CCAGCTGCAA GACGTAGACC CCGGTGGGGA CCTGGCCACT 
GTCAACAGCC AGCTCAAGAA GTTACAGTCC ATGGAGTCTC AGGTGGAGGA GTGGTGCCGA 
GAGGTGGGCG AGCTTCAGGC GCAGACGGCG GCGCTTCCGC TGGAGCAGGC GAGCAAGGAG 
TTGGTGGGGG AGCGGCAGAG TGCGGTGGGC GAGCGCCTGG TGCGCCTGCT GGAGCCTTTG 
CAGGAGCGAC GCCGCTTGTT GCTGGCATCC AAGGAGCTGC ATCAGGTGGC CCACGACCTG 
GACGATGAAC TGGCTTGGGT TCAAGAGCGG CTGCCACTGG CCATGCAGAC AGAGCGAGGC 
ACTGGCTTGC AGGCTGTCCA GCAGCACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC 
CAGGCTCACG GGCCGCGCTT GGAGGAGGTG CTGGAGCGCG CGGGCGTGCT GGCCTCGCTG 
CGCAGCCCAG AGGCCGAAGC GGTGCGCCGT GGCCAAGAGC AGCTGCAGAG CGCCTGGACC 
GGGCTGCGGG AAGCGGCCGA ACGGCGGCAG CAGACGCTGG ACGCCGCCTT CCAGGTGGAA 
CAGTACTACT TTGACGTGGC TGAGGTGGAG GCATGGCTGG GCGAGCAGGA GCTGCTCATG 
ATGAGTGAGG ACAAGGGCAA GGATGAACAG AGCACCCTGC AGCTGCTCAA GAAGCATTTA 
CAACTGGAAC AGGGCGTGGA GAACTATGAG GAAAGCATCG CCCAGCTGTC ACGCCAGTGC 
CGGGCGCTAC TGGAAATGGG ACACCCAGAC AGTGAGCAAA TCAGCCGCCG ACAGTCCCAA 
GTGGATCGCC TCTATGTGGC GCTCAAGGAG CTGGGCGAGG AACGCAGGGT GAGCCTGGAA 
CAGCAGTACT GGCTCTACCA GCTCAGCCGC CAGGTGGATG AGCTGGAACA CTGGATAGCC 
GAGAAGGAGG TGGTAGCTGG CTCCCCAGAG CTGGGCCAGG ACTTCGAACA CGTGTCGGTG 
CTACAGGAGA AATTCTCAGA GTTTGCCAGT GAGACAGGAA CCGCAGGGCG GGAGCGGCTG 
GCGGCGGTCA ACCAGATGGT GGACGAGCTG ATTGAGTGTG GTCACACAGC AGCGGCCACC 
ATGGCTGAGT GGAAGGACGG GCTGAACGAG GCCTGGGCTG AGCTGCTGGA ACTCATGGGC 
ACTCGGGCCC AGCTGCTCGC TGCCTCTCGG GAGCTGCATA AGTTCTTCAG CGATGCCCGG 
GAGCTTCAAG GGCAGATTGA GGAGAAGCGG AGGAGGCTGC CCCGCCTGAC GGCACCACCT 
GAACCCAGAC CCAGTGCCAG CTCCATGCAG CGGACCCTGC GAGCCTTTGA GCACGACCTG 
CAGCTCCTCG TGTCCCAGGT ACGGCAGCTG CAGGAAGGGG CGGCCCAGCT GCGGACGGTG 
TACGCTGGCG AGCACGCCGA GGCCATCGCC AGCCGCGAGC AGGAGGTGTT GCAGGGCTGG 
AAGGAGCTGC TAGCAGCCTG TGAAGATGCT CGTCTGCACG TCAGCTCCAC GGCCGACGCC 
CTGCGCTTCC ACAGCCAGGC CCGCGACCTG CTCTCCTGGA TGGACGGCAT CGCGGGCCAG 
ATCGGGGCAG CTGACAAACC CAGGGATGTG TCATCGGTGG AGGTGCTTAT GAACTACCAC 
CAGGGCCTGA AGACAGAGTT GGAGGCGCGC GTGCCTGAGC TGGCAACCTG CCAGGAGCTG 
GGGCGGTCTC TATTACTCAA CAAAAGCGCC ATGGCTGATG AGATCCAGGC CCAGCTGGAC 
AAGCTGGGAA GCCGGAAGGA GGAGGTGTCG GAGAAGTGGG ACCGTCACTG GGAGTGGCTG 
CAGCAGATGC TGGAGGTACA CCAGTTTGCT CAGGAGGCCG TGGTGGCTGA CGCCTGGCTG 
ACAGCTCAGG AACCGCTCCT GCAGAGCCGG GAGCTGGGCA GCAGTGTGGA TGAGGTGGAG 
CAGCTTATCC GGCGACACGA GGCCTTCCGA AAAGCAGCTG CGGCCTGGGA GGAGAGGTTC 
AGCTCTCTGC GGCGCTTGAC CACGATCGAG AAACTCAAGG CAGAACAGAG TAAGCAGCCA 
CCCACCCCCT TACTGGGGCG CAAGTTCTTT GGGGACCCCA CAGAGCTGGC GGCCAAGGCA 
GCGCCTTTGC TGCGTCCAGG GGGCTATGAC AGGGGCCTGG AGCCTTTGGC CCGCAGGGCC 
TCGGACACGC TGTCAGCGGA GGTGCGGACC CGGGTTGGGT ATGTGCGCCA GGAGCTCAAG 
CCCGAGCGTC TCCAGCCGCG CATTGACCGG CTGCCAGAGA CCTCGGGGAA GGTAGAACCC 
GCGGCCCCGA CAGCCGCAGC GTTGGACACG ACGGACACCC CTGGGACTCC GGCGGCGACG 
GAGTTGGTCC GGCCCCGGTC GGAGCGCCAG GAGTTAGCGG ATCGTGCGGA GGAGCTGCCA 
CGGAGACGAA GATCAGAACG CCAGGAGTCC GTGGATCAAC CAGAGGAGAC AGCGCGGAGG 
CGGCGGCCTG AGCGCCAGGA GTCCGCGGAC CACGAGGGGC CGCACAGCCT TACTCTAGGT 
CGCTACGAGC AGATGGAGAG ACGGCGGGAG CGGAGGGAGC GGCGGATAGA ACGACAGGAG 
TCTAGCGAAC AGGAGACTCC CACCAGGGGA GAGCTGGTCA AAGGGAAGGC CACTCTGGCT 
GATATTGTGG AGCAACTTCA GGAGAAAGAG GCAGGCCCAG GGATCCCTGC TGGGGTGCCG 
TCGCTGCCTC AGCCTCGCGA GCTTCCCCCT GGTCGCCTGC CCAACGGGCT TGAGCCGCCC 
GAGCGGACAC CTCGGCCCGA TCGGCCGCGG GCACGGGACC GGCCCAAGCC GCGCCGGCGG 
CCACGGCCTC GAGAGGGTGG TGAGGGCGGG GGAAGCCGGC GCTCGCGCTC CGCCCCTGCC 
CAGGGAGGCT CCGCCCCCGC GCCGCCACCT CCGCCCACTC ACACAGTGCA GCACGAGGGC 
TTCCTGCTGC GCAAGCGCGA GCTCGACGCT AACCGCAAGT CGTCCAACCG GTCATGGGTG 
AGCCTGTACT GTGTGCTCAG CAAGGGGGAG CTGGGCTTCT ACAAGGACTC CAAAGGCCCA 
GCATCAGGGG GCACGCATGG TGGGGAACCA CTGCTCAGCC TGCACAAAGC CACCAGCGAG 
GTGGCTAGTG ACTACAAGAA AAAGAAGCAT GTCTTCAAGC TCCAGACCCA GGATGGCAGT 
GAGTTCTTGC TCCAGGCTAA AGATGAGGAG GAGATGAACG GCTGGCTGGA GGCTGTGGCT 
AACTCCGTGG CAGAACACGC CGAGATCGCC CGATGGGGTC AGACACTACC TACCACATCA 
TCCACAGATG AGGGCAACCC CAAGCGTGAG GGCGGGGAGC GTAGGGCCAG CGGGCGCCGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115999.2
CDS	143..7828
Translation	MAQVPGEVDNMEGPAVSNNNNPSARWESPDRGWDREPPAAANAAASLFECSRIKALADEREAVQKKTFTKWVNSHLARVGCHIGDLYADLRDGFVLTRLLEVLSGEQLPRPTRGRMRIHSLENVDKALQFLKEQRVHLENVGSHDIVDGNHRLTLGLVWTIILRFQIQVIKIETEDNRETRSAKDALLLWCQMKTAGYPEVNIQNFTTSWRDGLAFNALIHRHRPDLVDLSKLTKSNANYNLQRAFRTAEQHLGLARLLDPEDVNMEAPDEKSIITYVVSFYHYFSKMKALAVEGKRIGKVLDQVLEVDKIIERYEELAAELLAWIHRTVDLISNQKFANSLSGVQQQLQAFTAYCTLEKPVKFQEKGNLEVLLFSIQSKLRAHNRRLFVPREGCGIWDIDKAWGELEKAEHEREAALRAELIRQEKLELLAQRFDHKVAMRESWLNENQRLVSQDNFGYELPAVEAAMKKHEAIEADIAAYEERVQGVAELAQALAAEGYYDARRVAAQRDSVLRQWALLTGLVGARRTRLEQNLALQKVFQEMVYMVDWMEEMQTQLLSRECGQHLVEADDLLQKHGLLEGDIAAQSERVEALNAAALRFSQLQGYQPCDPQVICNRVNHVHGCLSELQEQAARRRAELEASRSLWALLQELEEAESWARDKERLLEATSGSGGAAGTAGGAHDLSSTARLLAQHKILQGELGGRRALLQQALRRGEELAAAGGSVGPGAEPLHLAGLAERAASARRRWQRLEEAAARRERRLQEARALHQFGADLDGLLDWLRDAYRLAAAGDFGHDEASSRRLARQHRALTGEVEAHRGPVGGLRRQLATLGGASGAGPLVVALQVRVVEAEQLFAEVTEVAALRRQWLRDALAVYRMFGEVHACELWIGEKEQWLLAMRVPDSLDDVEVVQHRFESLDQEMNSLMGRVLDVNQTVQELVEGGHPSSDEVRSCQDHLNSRWNRIVELVEQRKEEMSAVLLVENHVLEVAEVRAQVREKRRAVESAPRAGGALQWRLSGLEAALQALEPRQAALLEEAALLAERFPAQATRLHQGAEELGAEWGALAGAAQACGEAVAAAGRLQRFLRDLDTFLDWLVRAQEAAGAVEGPLPRSLEEADGLLARHAALKEEVDQREEDYARIVAASEALLASEGAELGPGLALDEWLPHLEVGWHKLLGLWEERREALVQAHVYQLFLRDLCQALAVLRNQEVALSGAELPCTVESVEEAMKRHRDFLTTMELNQQKMQVAVQAAESLLRQGNAYGEQAQEAVARLLEKSQENQLRAQQWMQKLLDQLVLQHFLRDCHELDGWIHEKMLMARDGTREDSHKLHKRWLRHQAFMAELAQNKEWLEKIEREGQQLMQEKPELAASVRKKLGEIRQCWAELESTTQAKARQLFEASKADQLVQSFAELDKRLLHMESQLQDVDPGGDLATVNSQLKKLQSMESQVEEWCREVGELQAQTAALPLEQASKELVGERQSAVGERLVRLLEPLQERRRLLLASKELHQVAHDLDDELAWVQERLPLAMQTERGTGLQAVQQHIKKNQGLRREIQAHGPRLEEVLERAGVLASLRSPEAEAVRRGQEQLQSAWTGLREAAERRQQTLDAAFQVEQYYFDVAEVEAWLGEQELLMMSEDKGKDEQSTLQLLKKHLQLEQGVENYEESIAQLSRQCRALLEMGHPDSEQISRRQSQVDRLYVALKELGEERRVSLEQQYWLYQLSRQVDELEHWIAEKEVVAGSPELGQDFEHVSVLQEKFSEFASETGTAGRERLAAVNQMVDELIECGHTAAATMAEWKDGLNEAWAELLELMGTRAQLLAASRELHKFFSDARELQGQIEEKRRRLPRLTAPPEPRPSASSMQRTLRAFEHDLQLLVSQVRQLQEGAAQLRTVYAGEHAEAIASREQEVLQGWKELLAACEDARLHVSSTADALRFHSQARDLLSWMDGIAGQIGAADKPRDVSSVEVLMNYHQGLKTELEARVPELATCQELGRSLLLNKSAMADEIQAQLDKLGSRKEEVSEKWDRHWEWLQQMLEVHQFAQEAVVADAWLTAQEPLLQSRELGSSVDEVEQLIRRHEAFRKAAAAWEERFSSLRRLTTIEKLKAEQSKQPPTPLLGRKFFGDPTELAAKAAPLLRPGGYDRGLEPLARRASDTLSAEVRTRVGYVRQELKPERLQPRIDRLPETSGKVEPAAPTAAALDTTDTPGTPAATELVRPRSERQELADRAEELPRRRRSERQESVDQPEETARRRRPERQESADHEGPHSLTLGRYEQMERRRERRERRIERQESSEQETPTRGELVKGKATLADIVEQLQEKEAGPGIPAGVPSLPQPRELPPGRLPNGLEPPERTPRPDRPRARDRPKPRRRPRPREGGEGGGSRRSRSAPAQGGSAPAPPPPPTHTVQHEGFLLRKRELDANRKSSNRSWVSLYCVLSKGELGFYKDSKGPASGGTHGGEPLLSLHKATSEVASDYKKKKHVFKLQTQDGSEFLLQAKDEEEMNGWLEAVANSVAEHAEIARWGQTLPTTSSTDEGNPKREGGERRASGRRK

Target ORF information:

RefSeq Version	NM_032610.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant sigma1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032610.2

ORF Insert Sequence:

ATGGCACAAG TACCAGGGGA GGTGGACAAC ATGGAGGGCC CAGCAGTCTC CAACAACAAC 
AACCCTTCAG CTCGCTGGGA GAGCCCAGAT CGGGGCTGGG ATCGGGAACC TCCCGCAGCC 
GCCAATGCTG CGGCTTCACT CTTTGAGTGC TCTCGGATCA AGGCTTTAGC AGATGAGCGA 
GAGGCTGTGC AGAAGAAAAC CTTCACCAAG TGGGTGAATT CGCACCTTGC CCGCGTGGGC 
TGCCACATTG GAGACCTGTA TGCAGACCTT CGGGACGGGT TTGTCCTCAC CCGGCTTCTG 
GAAGTGCTGT CGGGGGAGCA GCTGCCGAGG CCCACTCGCG GCCGCATGCG GATCCACTCG 
CTGGAGAACG TGGACAAGGC TCTGCAGTTC CTGAAGGAGC AGCGTGTGCA TCTGGAGAAC 
GTGGGGTCAC ATGACATCGT GGATGGGAAC CACCGACTGA CGCTGGGGCT GGTCTGGACC 
ATCATCCTGC GCTTCCAGAT TCAAGTCATC AAGATTGAGA CAGAAGACAA CAGAGAGACC 
CGCTCCGCCA AGGACGCTCT GCTCTTGTGG TGCCAGATGA AAACAGCCGG TTACCCTGAG 
GTAAACATCC AGAATTTCAC CACCAGCTGG AGAGATGGCC TGGCCTTCAA TGCCCTCATT 
CACCGTCACA GGCCTGATCT TGTGGACCTC AGCAAACTTA CCAAGTCCAA CGCCAACTAC 
AACCTGCAGA GAGCTTTCCG CACAGCCGAG CAGCACCTGG GGCTAGCACG TCTGCTGGAC 
CCCGAAGATG TGAACATGGA GGCTCCAGAT GAGAAGTCCA TTATCACCTA TGTGGTCTCC 
TTCTACCACT ACTTCTCCAA GATGAAGGCT CTGGCGGTGG AGGGAAAACG GATTGGAAAG 
GTCCTGGACC AGGTGTTGGA GGTGGACAAG ATCATAGAAC GCTATGAGGA GCTGGCGGCG 
GAGCTGTTGG CCTGGATCCA TCGCACTGTG GACCTCATTA GCAACCAAAA GTTTGCTAAC 
TCACTGAGTG GGGTGCAGCA GCAGCTGCAG GCCTTCACGG CTTACTGTAC GCTGGAGAAG 
CCCGTCAAGT TTCAGGAGAA GGGGAACCTG GAGGTGCTAC TCTTCAGCAT CCAAAGCAAA 
CTGCGCGCCC ACAACCGGCG CCTCTTTGTG CCTCGAGAAG GCTGTGGCAT CTGGGATATT 
GACAAGGCGT GGGGTGAGCT GGAGAAGGCA GAGCATGAGC GAGAGGCGGC CCTCAGAGCT 
GAGCTCATCC GGCAGGAGAA GTTGGAACTC TTGGCTCAAA GATTTGACCA CAAGGTGGCC 
ATGAGGGAGA GCTGGCTAAA TGAGAACCAG CGCCTGGTCT CCCAGGACAA CTTCGGATAT 
GAGCTGCCGG CAGTGGAGGC AGCCATGAAG AAGCACGAAG CCATTGAGGC AGACATTGCA 
GCCTACGAGG AGCGGGTTCA GGGTGTGGCG GAGTTAGCCC AGGCGTTGGC AGCTGAAGGC 
TACTATGATG CCCGGCGGGT GGCTGCCCAG CGTGACAGTG TCCTTCGCCA GTGGGCCCTG 
CTGACCGGGC TGGTAGGTGC CCGGCGGACC CGGCTTGAGC AGAATCTGGC CCTGCAGAAG 
GTCTTCCAGG AGATGGTCTA CATGGTGGAC TGGATGGAGG AGATGCAGAC TCAGCTGCTG 
TCTCGGGAAT GTGGGCAGCA CCTGGTGGAG GCCGATGACC TGCTGCAGAA GCACGGGCTG 
CTGGAGGGGG ACATCGCAGC CCAGAGTGAG CGAGTGGAGG CCCTCAATGC TGCAGCCCTG 
AGATTCTCCC AGCTACAGGG CTATCAGCCC TGCGACCCTC AGGTCATTTG CAACCGTGTG 
AACCACGTGC ATGGCTGTCT GTCGGAGCTG CAGGAGCAGG CAGCACGGCG GCGCGCAGAG 
CTGGAAGCTT CTCGGAGCCT GTGGGCGCTG CTGCAGGAGC TGGAGGAGGC GGAGAGTTGG 
GCGCGGGACA AGGAGCGCCT CCTAGAGGCC ACGAGCGGCA GTGGTGGCGC GGCGGGCACG 
GCGGGCGGCG CGCACGACCT GTCCAGCACC GCTCGCCTGC TGGCGCAGCA TAAGATCCTG 
CAGGGCGAGC TGGGCGGCCG GCGGGCGCTG CTGCAGCAGG CGCTGCGACG CGGGGAGGAG 
TTGGCTGCGG CGGGCGGCTC CGTGGGCCCG GGCGCCGAGC CTTTGCATTT GGCTGGCCTG 
GCGGAGCGCG CGGCTAGCGC TCGGCGCCGC TGGCAGCGGC TGGAAGAGGC GGCTGCGCGC 
CGGGAACGGC GGCTGCAGGA GGCGCGGGCG CTGCACCAAT TTGGCGCCGA CCTGGACGGG 
CTGCTGGACT GGCTTCGTGA CGCGTATCGT CTAGCAGCCG CTGGAGATTT TGGTCACGAC 
GAAGCGTCCA GCCGTCGCCT GGCGCGCCAG CACCGCGCTC TCACCGGGGA GGTGGAAGCG 
CACCGTGGCC CTGTGGGTGG CCTGAGGCGC CAGCTGGCGA CACTGGGAGG AGCCAGTGGC 
GCAGGGCCGC TGGTGGTGGC TCTGCAGGTG CGCGTGGTGG AAGCCGAACA GTTGTTCGCC 
GAGGTGACCG AGGTGGCCGC GCTGCGACGA CAGTGGCTTC GGGACGCGCT CGCCGTGTAC 
CGCATGTTCG GCGAGGTACA TGCCTGCGAG TTGTGGATCG GGGAGAAAGA GCAGTGGCTG 
CTGGCCATGC GTGTGCCCGA TTCCCTCGAC GACGTTGAGG TGGTGCAGCA TCGATTTGAA 
AGTCTTGACC AGGAAATGAA CAGCCTGATG GGCCGAGTCC TGGATGTGAA CCAAACAGTT 
CAGGAGTTGG TAGAAGGAGG TCATCCCAGC TCCGATGAGG TGCGCTCCTG TCAGGACCAT 
CTCAACAGCA GGTGGAACCG CATCGTGGAG TTGGTGGAAC AGCGCAAAGA GGAGATGAGC 
GCGGTGCTGC TGGTGGAGAA CCACGTGCTG GAGGTGGCCG AGGTGCGCGC CCAAGTGCGC 
GAGAAGCGAC GGGCAGTGGA GAGCGCGCCC CGGGCGGGGG GAGCCCTGCA ATGGCGCCTG 
AGTGGCCTGG AGGCGGCTCT ACAGGCGCTA GAGCCGCGAC AGGCGGCGCT GCTGGAGGAG 
GCGGCGCTGC TGGCCGAACG CTTCCCCGCA CAGGCGACGC GCCTGCACCA GGGCGCCGAG 
GAGCTAGGAG CCGAGTGGGG CGCACTGGCC GGAGCGGCTC AGGCCTGCGG CGAGGCGGTT 
GCGGCGGCAG GTCGCCTGCA GCGTTTCCTG CGCGATTTGG ATACTTTCCT GGACTGGCTG 
GTGCGGGCGC AGGAGGCAGC AGGCGCCGTC GAGGGGCCGC TGCCTCGCAG CCTGGAGGAG 
GCGGATGGGC TGCTGGCGCG CCACGCCGCG CTCAAGGAGG AGGTGGATCA GCGTGAGGAG 
GACTACGCGC GCATCGTGGC TGCCAGTGAA GCGCTGCTAG CGTCCGAGGG CGCGGAGCTG 
GGCCCCGGTC TGGCGCTAGA CGAGTGGCTG CCGCACCTCG AGGTTGGCTG GCACAAACTG 
CTCGGCTTGT GGGAGGAGCG CAGGGAGGCG CTGGTCCAAG CGCACGTCTA TCAACTCTTC 
CTGAGAGACC TGTGCCAGGC GCTTGCGGTG CTGCGCAACC AGGAGGTGGC GCTGTCTGGC 
GCTGAACTCC CTTGCACAGT GGAGTCGGTA GAAGAGGCGA TGAAAAGGCA CCGGGATTTT 
CTCACCACGA TGGAGCTGAA CCAGCAAAAG ATGCAGGTGG CGGTTCAAGC TGCAGAGAGC 
CTGCTGCGGC AAGGCAACGC CTACGGGGAG CAGGCGCAGG AGGCAGTGGC TCGGCTGCTA 
GAGAAGAGCC AAGAAAATCA GCTGAGGGCC CAGCAGTGGA TGCAAAAACT ACTGGACCAG 
CTTGTGCTGC AGCACTTTCT TAGAGATTGT CATGAGCTGG ATGGCTGGAT CCATGAGAAG 
ATGCTGATGG CTCGTGATGG CACCCGGGAA GACAGCCACA AGCTGCACAA GAGATGGCTC 
CGGCACCAGG CGTTCATGGC TGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATAGAA 
AGGGAGGGCC AGCAACTGAT GCAGGAGAAA CCGGAGCTGG CAGCCTCTGT GCGCAAGAAA 
TTAGGTGAGA TCCGGCAGTG CTGGGCGGAG CTGGAGAGCA CCACACAGGC CAAGGCGCGC 
CAGCTCTTCG AGGCCAGCAA GGCGGACCAG CTAGTGCAGA GCTTCGCGGA GCTGGACAAG 
CGGCTGCTTC ACATGGAAAG CCAGCTGCAA GACGTAGACC CCGGTGGGGA CCTGGCCACT 
GTCAACAGCC AGCTCAAGAA GTTACAGTCC ATGGAGTCTC AGGTGGAGGA GTGGTGCCGA 
GAGGTGGGCG AGCTTCAGGC GCAGACGGCG GCGCTTCCGC TGGAGCAGGC GAGCAAGGAG 
TTGGTGGGGG AGCGGCAGAG TGCGGTGGGC GAGCGCCTGG TGCGCCTGCT GGAGCCTTTG 
CAGGAGCGAC GCCGCTTGTT GCTGGCATCC AAGGAGCTGC ATCAGGTGGC CCACGACCTG 
GACGATGAAC TGGCTTGGGT TCAAGAGCGG CTGCCACTGG CCATGCAGAC AGAGCGAGGC 
ACTGGCTTGC AGGCTGTCCA GCAGCACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC 
CAGGCTCACG GGCCGCGCTT GGAGGAGGTG CTGGAGCGCG CGGGCGTGCT GGCCTCGCTG 
CGCAGCCCAG AGGCCGAAGC GGTGCGCCGT GGCCAAGAGC AGCTGCAGAG CGCCTGGACC 
GGGCTGCGGG AAGCGGCCGA ACGGCGGCAG CAGACGCTGG ACGCCGCCTT CCAGGTGGAA 
CAGTACTACT TTGACGTGGC TGAGGTGGAG GCATGGCTGG GCGAGCAGGA GCTGCTCATG 
ATGAGTGAGG ACAAGGGCAA GGATGAACAG AGCACCCTGC AGCTGCTCAA GAAGCATTTA 
CAACTGGAAC AGGGCGTGGA GAACTATGAG GAAAGCATCG CCCAGCTGTC ACGCCAGTGC 
CGGGCGCTAC TGGAAATGGG ACACCCAGAC AGTGAGCAAA TCAGCCGCCG ACAGTCCCAA 
GTGGATCGCC TCTATGTGGC GCTCAAGGAG CTGGGCGAGG AACGCAGGGT GAGCCTGGAA 
CAGCAGTACT GGCTCTACCA GCTCAGCCGC CAGGTGGATG AGCTGGAACA CTGGATAGCC 
GAGAAGGAGG TGGTAGCTGG CTCCCCAGAG CTGGGCCAGG ACTTCGAACA CGTGTCGGTG 
CTACAGGAGA AATTCTCAGA GTTTGCCAGT GAGACAGGAA CCGCAGGGCG GGAGCGGCTG 
GCGGCGGTCA ACCAGATGGT GGACGAGCTG ATTGAGTGTG GTCACACAGC AGCGGCCACC 
ATGGCTGAGT GGAAGGACGG GCTGAACGAG GCCTGGGCTG AGCTGCTGGA ACTCATGGGC 
ACTCGGGCCC AGCTGCTCGC TGCCTCTCGG GAGCTGCATA AGTTCTTCAG CGATGCCCGG 
GAGCTTCAAG GGCAGATTGA GGAGAAGCGG AGGAGGCTGC CCCGCCTGAC GGCACCACCT 
GAACCCAGAC CCAGTGCCAG CTCCATGCAG CGGACCCTGC GAGCCTTTGA GCACGACCTG 
CAGCTCCTCG TGTCCCAGGT ACGGCAGCTG CAGGAAGGGG CGGCCCAGCT GCGGACGGTG 
TACGCTGGCG AGCACGCCGA GGCCATCGCC AGCCGCGAGC AGGAGGTGTT GCAGGGCTGG 
AAGGAGCTGC TAGCAGCCTG TGAAGATGCT CGTCTGCACG TCAGCTCCAC GGCCGACGCC 
CTGCGCTTCC ACAGCCAGGC CCGCGACCTG CTCTCCTGGA TGGACGGCAT CGCGGGCCAG 
ATCGGGGCAG CTGACAAACC CAGGGATGTG TCATCGGTGG AGGTGCTTAT GAACTACCAC 
CAGGGCCTGA AGACAGAGTT GGAGGCGCGC GTGCCTGAGC TGGCAACCTG CCAGGAGCTG 
GGGCGGTCTC TATTACTCAA CAAAAGCGCC ATGGCTGATG AGATCCAGGC CCAGCTGGAC 
AAGCTGGGAA GCCGGAAGGA GGAGGTGTCG GAGAAGTGGG ACCGTCACTG GGAGTGGCTG 
CAGCAGATGC TGGAGGTACA CCAGTTTGCT CAGGAGGCCG TGGTGGCTGA CGCCTGGCTG 
ACAGCTCAGG AACCGCTCCT GCAGAGCCGG GAGCTGGGCA GCAGTGTGGA TGAGGTGGAG 
CAGCTTATCC GGCGACACGA GGCCTTCCGA AAAGCAGCTG CGGCCTGGGA GGAGAGGTTC 
AGCTCTCTGC GGCGCTTGAC CACGATCGAG AAACTCAAGG CAGAACAGAG TAAGCAGCCA 
CCCACCCCCT TACTGGGGCG CAAGTTCTTT GGGGACCCCA CAGAGCTGGC GGCCAAGGCA 
GCGCCTTTGC TGCGTCCAGG GGGCTATGAC AGGGGCCTGG AGCCTTTGGC CCGCAGGGCC 
TCGGACACGC TGTCAGCGGA GGTGCGGACC CGGGTTGGGT ATGTGCGCCA GGAGCTCAAG 
CCCGAGCGTC TCCAGCCGCG CATTGACCGG CTGCCAGAGA CCTCGGGGAA GGTAGAACCC 
GCGGCCCCGA CAGCCGCAGC GTTGGACACG ACGGACACCC CTGGGACTCC GGCGGCGACG 
GAGTTGGTCC GGCCCCGGTC GGAGCGCCAG GAGTTAGCGG ATCGTGCGGA GGAGCTGCCA 
CGGAGACGAA GATCAGAACG CCAGGAGTCC GTGGATCAAC CAGAGGAGAC AGCGCGGAGG 
CGGCGGCCTG AGCGCCAGGA GTCCGCGGAC CACGAGGGGC CGCACAGCCT TACTCTAGGT 
CGCTACGAGC AGATGGAGAG ACGGCGGGAG CGGAGGGAGC GGCGGATAGA ACGACAGGAG 
TCTAGCGAAC AGGAGACTCC CACCAGGGGA GAGCTGGTCA AAGGGAAGGC CACTCTGGCT 
GATATTGTGG AGCAACTTCA GGAGAAAGAG GCAGGCCCAG GGATCCCTGC TGGGGTGCCG 
TCGCTGCCTC AGCCTCGCGA GCTTCCCCCT GGTCGCCTGC CCAACGGGCT TGAGCCGCCC 
GAGCGGACAC CTCGGCCCGA TCGGCCGCGG GCACGGGACC GGCCCAAGCC GCGCCGGCGG 
CCACGGCCTC GAGAGGGTGG TGAGGGCGGG GGAAGCCGGC GCTCGCGCTC CGCCCCTGCC 
CAGGGAGGCT CCGCCCCCGC GCCGCCACCT CCGCCCACTC ACACAGTGCA GCACGAGGGC 
TTCCTGCTGC GCAAGCGCGA GCTCGACGCT AACCGCAAGT CGTCCAACCG GTCATGGGTG 
AGCCTGTACT GTGTGCTCAG CAAGGGGGAG CTGGGCTTCT ACAAGGACTC CAAAGGCCCA 
GCATCAGGGG GCACGCATGG TGGGGAACCA CTGCTCAGCC TGCACAAAGC CACCAGCGAG 
GTGGCTAGTG ACTACAAGAA AAAGAAGCAT GTCTTCAAGC TCCAGACCCA GGATGGCAGT 
GAGTTCTTGC TCCAGGCTAA AGATGAGGAG GAGATGAACG GCTGGCTGGA GGCTGTGGCT 
AACTCCGTGG CAGAACACGC CGAGATCGCC CGATGGGGTC AGACACTACC TACCACATCA 
TCCACAGATG AGGGCAACCC CAAGCGTGAG GGCGGGGAGC GTAGGGCCAG CGGGCGCCGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn4 ( XM_006540436.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540499.1 Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant X1, mRNA.

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CloneID	OMu38545
Clone ID Related Accession (Same CDS sequence)	XM_006540436.3 , XM_006540436.2
Accession Version	XM_006540436.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006540436.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGATCT GGCCGAGCGG CGGGCCGGTG GGGCCGAACA GAGCCGGGCC CGGCCCGGCG 
ACGAACGCCC GCAGGGAGGG CGGTGCTGCG CGGCGAGCAT CCGGGACCGC ATCGGCGGCC 
GGCGCCCAGG CCCTGCCTCC CCCAATGGCA CAAGTACCAG GGGAGGTGGA CAACATGGAG 
GGCCCAGCAG TCTCCAACAA CAACAACCCT TCAGCTCGCT GGGAGAGCCC AGATCGGGGC 
TGGGATCGGG AACCTCCCGC AGCCGCCAAT GCTGCGGCTT CACTCTTTGA GTGCTCTCGG 
ATCAAGGCTT TAGCAGATGA GCGAGAGGCT GTGCAGAAGA AAACCTTCAC CAAGTGGGTG 
AATTCGCACC TTGCCCGCGT GGGCTGCCAC ATTGGAGACC TGTATGCAGA CCTTCGGGAC 
GGGTTTGTCC TCACCCGGCT TCTGGAAGTG CTGTCGGGGG AGCAGCTGCC GAGGCCCACT 
CGCGGCCGCA TGCGGATCCA CTCGCTGGAG AACGTGGACA AGGCTCTGCA GTTCCTGAAG 
GAGCAGCGTG TGCATCTGGA GAACGTGGGG TCACATGACA TCGTGGATGG GAACCACCGA 
CTGACGCTGG GGCTGGTCTG GACCATCATC CTGCGCTTCC AGATTCAAGT CATCAAGATT 
GAGACAGAAG ACAACAGAGA GACCCGCTCC GCCAAGGACG CTCTGCTCTT GTGGTGCCAG 
ATGAAAACAG CCGGTTACCC TGAGGTAAAC ATCCAGAATT TCACCACCAG CTGGAGAGAT 
GGCCTGGCCT TCAATGCCCT CATTCACCGT CACAGGCCTG ATCTTGTGGA CCTCAGCAAA 
CTTACCAAGT CCAACGCCAA CTACAACCTG CAGAGAGCTT TCCGCACAGC CGAGCAGCAC 
CTGGGGCTAG CACGTCTGCT GGACCCCGAA GATGTGAACA TGGAGGCTCC AGATGAGAAG 
TCCATTATCA CCTATGTGGT CTCCTTCTAC CACTACTTCT CCAAGATGAA GGCTCTGGCG 
GTGGAGGGAA AACGGATTGG AAAGGTCCTG GACCAGGTGT TGGAGGTGGA CAAGATCATA 
GAACGCTATG AGGAGCTGGC GGCGGAGCTG TTGGCCTGGA TCCATCGCAC TGTGGACCTC 
ATTAGCAACC AAAAGTTTGC TAACTCACTG AGTGGGGTGC AGCAGCAGCT GCAGGCCTTC 
ACGGCTTACT GTACGCTGGA GAAGCCCGTC AAGTTTCAGG AGAAGGGGAA CCTGGAGGTG 
CTACTCTTCA GCATCCAAAG CAAACTGCGC GCCCACAACC GGCGCCTCTT TGTGCCTCGA 
GAAGGCTGTG GCATCTGGGA TATTGACAAG GCGTGGGGTG AGCTGGAGAA GGCAGAGCAT 
GAGCGAGAGG CGGCCCTCAG AGCTGAGCTC ATCCGGCAGG AGAAGTTGGA ACTCTTGGCT 
CAAAGATTTG ACCACAAGGT GGCCATGAGG GAGAGCTGGC TAAATGAGAA CCAGCGCCTG 
GTCTCCCAGG ACAACTTCGG ATATGAGCTG CCGGCAGTGG AGGCAGCCAT GAAGAAGCAC 
GAAGCCATTG AGGCAGACAT TGCAGCCTAC GAGGAGCGGG TTCAGGGTGT GGCGGAGTTA 
GCCCAGGCGT TGGCAGCTGA AGGCTACTAT GATGCCCGGC GGGTGGCTGC CCAGCGTGAC 
AGTGTCCTTC GCCAGTGGGC CCTGCTGACC GGGCTGGTAG GTGCCCGGCG GACCCGGCTT 
GAGCAGAATC TGGCCCTGCA GAAGGTCTTC CAGGAGATGG TCTACATGGT GGACTGGATG 
GAGGAGATGC AGACTCAGCT GCTGTCTCGG GAATGTGGGC AGCACCTGGT GGAGGCCGAT 
GACCTGCTGC AGAAGCACGG GCTGCTGGAG GGGGACATCG CAGCCCAGAG TGAGCGAGTG 
GAGGCCCTCA ATGCTGCAGC CCTGAGATTC TCCCAGCTAC AGGGCTATCA GCCCTGCGAC 
CCTCAGGTCA TTTGCAACCG TGTGAACCAC GTGCATGGCT GTCTGTCGGA GCTGCAGGAG 
CAGGCAGCAC GGCGGCGCGC AGAGCTGGAA GCTTCTCGGA GCCTGTGGGC GCTGCTGCAG 
GAGCTGGAGG AGGCGGAGAG TTGGGCGCGG GACAAGGAGC GCCTCCTAGA GGCCACGAGC 
GGCAGTGGTG GCGCGGCGGG CACGGCGGGC GGCGCGCACG ACCTGTCCAG CACCGCTCGC 
CTGCTGGCGC AGCATAAGAT CCTGCAGGGC GAGCTGGGCG GCCGGCGGGC GCTGCTGCAG 
CAGGCGCTGC GACGCGGGGA GGAGTTGGCT GCGGCGGGCG GCTCCGTGGG CCCGGGCGCC 
GAGCCTTTGC ATTTGGCTGG CCTGGCGGAG CGCGCGGCTA GCGCTCGGCG CCGCTGGCAG 
CGGCTGGAAG AGGCGGCTGC GCGCCGGGAA CGGCGGCTGC AGGAGGCGCG GGCGCTGCAC 
CAATTTGGCG CCGACCTGGA CGGGCTGCTG GACTGGCTTC GTGACGCGTA TCGTCTAGCA 
GCCGCTGGAG ATTTTGGTCA CGACGAAGCG TCCAGCCGTC GCCTGGCGCG CCAGCACCGC 
GCTCTCACCG GGGAGGTGGA AGCGCACCGT GGCCCTGTGG GTGGCCTGAG GCGCCAGCTG 
GCGACACTGG GAGGAGCCAG TGGCGCAGGG CCGCTGGTGG TGGCTCTGCA GGTGCGCGTG 
GTGGAAGCCG AACAGTTGTT CGCCGAGGTG ACCGAGGTGG CCGCGCTGCG ACGACAGTGG 
CTTCGGGACG CGCTCGCCGT GTACCGCATG TTCGGCGAGG TACATGCCTG CGAGTTGTGG 
ATCGGGGAGA AAGAGCAGTG GCTGCTGGCC ATGCGTGTGC CCGATTCCCT CGACGACGTT 
GAGGTGGTGC AGCATCGATT TGAAAGTCTT GACCAGGAAA TGAACAGCCT GATGGGCCGA 
GTCCTGGATG TGAACCAAAC AGTTCAGGAG TTGGTAGAAG GAGGTCATCC CAGCTCCGAT 
GAGGTGCGCT CCTGTCAGGA CCATCTCAAC AGCAGGTGGA ACCGCATCGT GGAGTTGGTG 
GAACAGCGCA AAGAGGAGAT GAGCGCGGTG CTGCTGGTGG AGAACCACGT GCTGGAGGTG 
GCCGAGGTGC GCGCCCAAGT GCGCGAGAAG CGACGGGCAG TGGAGAGCGC GCCCCGGGCG 
GGGGGAGCCC TGCAATGGCG CCTGAGTGGC CTGGAGGCGG CTCTACAGGC GCTAGAGCCG 
CGACAGGCGG CGCTGCTGGA GGAGGCGGCG CTGCTGGCCG AACGCTTCCC CGCACAGGCG 
ACGCGCCTGC ACCAGGGCGC CGAGGAGCTA GGAGCCGAGT GGGGCGCACT GGCCGGAGCG 
GCTCAGGCCT GCGGCGAGGC GGTTGCGGCG GCAGGTCGCC TGCAGCGTTT CCTGCGCGAT 
TTGGATACTT TCCTGGACTG GCTGGTGCGG GCGCAGGAGG CAGCAGGCGC CGTCGAGGGG 
CCGCTGCCTC GCAGCCTGGA GGAGGCGGAT GGGCTGCTGG CGCGCCACGC CGCGCTCAAG 
GAGGAGGTGG ATCAGCGTGA GGAGGACTAC GCGCGCATCG TGGCTGCCAG TGAAGCGCTG 
CTAGCGTCCG AGGGCGCGGA GCTGGGCCCC GGTCTGGCGC TAGACGAGTG GCTGCCGCAC 
CTCGAGGTTG GCTGGCACAA ACTGCTCGGC TTGTGGGAGG AGCGCAGGGA GGCGCTGGTC 
CAAGCGCACG TCTATCAACT CTTCCTGAGA GACCTGTGCC AGGCGCTTGC GGTGCTGCGC 
AACCAGGAGG TGGCGCTGTC TGGCGCTGAA CTCCCTTGCA CAGTGGAGTC GGTAGAAGAG 
GCGATGAAAA GGCACCGGGA TTTTCTCACC ACGATGGAGC TGAACCAGCA AAAGATGCAG 
GTGGCGGTTC AAGCTGCAGA GAGCCTGCTG CGGCAAGGCA ACGCCTACGG GGAGCAGGCG 
CAGGAGGCAG TGGCTCGGCT GCTAGAGAAG AGCCAAGAAA ATCAGCTGAG GGCCCAGCAG 
TGGATGCAAA AACTACTGGA CCAGCTTGTG CTGCAGCACT TTCTTAGAGA TTGTCATGAG 
CTGGATGGCT GGATCCATGA GAAGATGCTG ATGGCTCGTG ATGGCACCCG GGAAGACAGC 
CACAAGCTGC ACAAGAGATG GCTCCGGCAC CAGGCGTTCA TGGCTGAGCT GGCTCAGAAT 
AAGGAGTGGC TGGAGAAGAT AGAAAGGGAG GGCCAGCAAC TGATGCAGGA GAAACCGGAG 
CTGGCAGCCT CTGTGCGCAA GAAATTAGGT GAGATCCGGC AGTGCTGGGC GGAGCTGGAG 
AGCACCACAC AGGCCAAGGC GCGCCAGCTC TTCGAGGCCA GCAAGGCGGA CCAGCTAGTG 
CAGAGCTTCG CGGAGCTGGA CAAGCGGCTG CTTCACATGG AAAGCCAGCT GCAAGACGTA 
GACCCCGGTG GGGACCTGGC CACTGTCAAC AGCCAGCTCA AGAAGTTACA GTCCATGGAG 
TCTCAGGTGG AGGAGTGGTG CCGAGAGGTG GGCGAGCTTC AGGCGCAGAC GGCGGCGCTT 
CCGCTGGAGC AGGCGAGCAA GGAGTTGGTG GGGGAGCGGC AGAGTGCGGT GGGCGAGCGC 
CTGGTGCGCC TGCTGGAGCC TTTGCAGGAG CGACGCCGCT TGTTGCTGGC ATCCAAGGAG 
CTGCATCAGG TGGCCCACGA CCTGGACGAT GAACTGGCTT GGGTTCAAGA GCGGCTGCCA 
CTGGCCATGC AGACAGAGCG AGGCACTGGC TTGCAGGCTG TCCAGCAGCA CATCAAAAAG 
AACCAGGGCC TGCGGCGGGA GATCCAGGCT CACGGGCCGC GCTTGGAGGA GGTGCTGGAG 
CGCGCGGGCG TGCTGGCCTC GCTGCGCAGC CCAGAGGCCG AAGCGGTGCG CCGTGGCCAA 
GAGCAGCTGC AGAGCGCCTG GACCGGGCTG CGGGAAGCGG CCGAACGGCG GCAGCAGACG 
CTGGACGCCG CCTTCCAGGT GGAACAGTAC TACTTTGACG TGGCTGAGGT GGAGGCATGG 
CTGGGCGAGC AGGAGCTGCT CATGATGAGT GAGGACAAGG GCAAGGATGA ACAGAGCACC 
CTGCAGCTGC TCAAGAAGCA TTTACAACTG GAACAGGGCG TGGAGAACTA TGAGGAAAGC 
ATCGCCCAGC TGTCACGCCA GTGCCGGGCG CTACTGGAAA TGGGACACCC AGACAGTGAG 
CAAATCAGCC GCCGACAGTC CCAAGTGGAT CGCCTCTATG TGGCGCTCAA GGAGCTGGGC 
GAGGAACGCA GGGTGAGCCT GGAACAGCAG TACTGGCTCT ACCAGCTCAG CCGCCAGGTG 
GATGAGCTGG AACACTGGAT AGCCGAGAAG GAGGTGGTAG CTGGCTCCCC AGAGCTGGGC 
CAGGACTTCG AACACGTGTC GGTGCTACAG GAGAAATTCT CAGAGTTTGC CAGTGAGACA 
GGAACCGCAG GGCGGGAGCG GCTGGCGGCG GTCAACCAGA TGGTGGACGA GCTGATTGAG 
TGTGGTCACA CAGCAGCGGC CACCATGGCT GAGTGGAAGG ACGGGCTGAA CGAGGCCTGG 
GCTGAGCTGC TGGAACTCAT GGGCACTCGG GCCCAGCTGC TCGCTGCCTC TCGGGAGCTG 
CATAAGTTCT TCAGCGATGC CCGGGAGCTT CAAGGGCAGA TTGAGGAGAA GCGGAGGAGG 
CTGCCCCGCC TGACGGCACC ACCTGAACCC AGACCCAGTG CCAGCTCCAT GCAGCGGACC 
CTGCGAGCCT TTGAGCACGA CCTGCAGCTC CTCGTGTCCC AGGTACGGCA GCTGCAGGAA 
GGGGCGGCCC AGCTGCGGAC GGTGTACGCT GGCGAGCACG CCGAGGCCAT CGCCAGCCGC 
GAGCAGGAGG TGTTGCAGGG CTGGAAGGAG CTGCTAGCAG CCTGTGAAGA TGCTCGTCTG 
CACGTCAGCT CCACGGCCGA CGCCCTGCGC TTCCACAGCC AGGCCCGCGA CCTGCTCTCC 
TGGATGGACG GCATCGCGGG CCAGATCGGG GCAGCTGACA AACCCAGGGA TGTGTCATCG 
GTGGAGGTGC TTATGAACTA CCACCAGGGC CTGAAGACAG AGTTGGAGGC GCGCGTGCCT 
GAGCTGGCAA CCTGCCAGGA GCTGGGGCGG TCTCTATTAC TCAACAAAAG CGCCATGGCT 
GATGAGATCC AGGCCCAGCT GGACAAGCTG GGAAGCCGGA AGGAGGAGGT GTCGGAGAAG 
TGGGACCGTC ACTGGGAGTG GCTGCAGCAG ATGCTGGAGG TACACCAGTT TGCTCAGGAG 
GCCGTGGTGG CTGACGCCTG GCTGACAGCT CAGGAACCGC TCCTGCAGAG CCGGGAGCTG 
GGCAGCAGTG TGGATGAGGT GGAGCAGCTT ATCCGGCGAC ACGAGGCCTT CCGAAAAGCA 
GCTGCGGCCT GGGAGGAGAG GTTCAGCTCT CTGCGGCGCT TGACCACGAT CGAGAAACTC 
AAGGCAGAAC AGAGTAAGCA GCCACCCACC CCCTTACTGG GGCGCAAGTT CTTTGGGGAC 
CCCACAGAGC TGGCGGCCAA GGCAGCGCCT TTGCTGCGTC CAGGGGGCTA TGACAGGGGC 
CTGGAGCCTT TGGCCCGCAG GGCCTCGGAC ACGCTGTCAG CGGAGGTGCG GACCCGGGTT 
GGGTATGTGC GCCAGGAGCT CAAGCCCGAG CGTCTCCAGC CGCGCATTGA CCGGCTGCCA 
GAGACCTCGG GGAAGGTAGA ACCCGCGGCC CCGACAGCCG CAGCGTTGGA CACGACGGAC 
ACCCCTGGGA CTCCGGCGGC GACGGAGTTG GTCCGGCCCC GGTCGGAGCG CCAGGAGTTA 
GCGGATCGTG CGGAGGAGCT GCCACGGAGA CGAAGATCAG AACGCCAGGA GTCCGTGGAT 
CAACCAGAGG AGACAGCGCG GAGGCGGCGG CCTGAGCGCC AGGAGTCCGC GGACCACGAG 
GGGCCGCACA GCCTTACTCT AGGTCGCTAC GAGCAGATGG AGAGACGGCG GGAGCGGAGG 
GAGCGGCGGA TAGAACGACA GGAGTCTAGC GAACAGGAGA CTCCCACCAG GGGAGAGCTG 
GTCAAAGGGA AGGCCACTCT GGCTGATATT GTGGAGCAAC TTCAGGAGAA AGAGGCAGGC 
CCAGGGATCC CTGCTGGGGT GCCGTCGCTG CCTCAGCCTC GCGAGCTTCC CCCTGGTCGC 
CTGCCCAACG GGCTTGAGCC GCCCGAGCGG ACACCTCGGC CCGATCGGCC GCGGGCACGG 
GACCGGCCCA AGCCGCGCCG GCGGCCACGG CCTCGAGAGG GTGGTGAGGG CGGGGGAAGC 
CGGCGCTCGC GCTCCGCCCC TGCCCAGGGA GGCTCCGCCC CCGCGCCGCC ACCTCCGCCC 
ACTCACACAG TGCAGCACGA GGGCTTCCTG CTGCGCAAGC GCGAGCTCGA CGCTAACCGC 
AAGTCGTCCA ACCGGTCATG GGTGAGCCTG TACTGTGTGC TCAGCAAGGG GGAGCTGGGC 
TTCTACAAGG ACTCCAAAGG CCCAGCATCA GGGGGCACGC ATGGTGGGGA ACCACTGCTC 
AGCCTGCACA AAGCCACCAG CGAGGTGGCT AGTGACTACA AGAAAAAGAA GCATGTCTTC 
AAGCTCCAGA CCCAGGATGG CAGTGAGTTC TTGCTCCAGG CTAAAGATGA GGAGGAGATG 
AACGGCTGGC TGGAGGCTGT GGCTAACTCC GTGGCAGAAC ACGCCGAGAT CGCCCGATGG 
GGTCAGACAC TACCTACCAC ATCATCCACA GATGAGGGCA ACCCCAAGCG TGAGGGCGGG 
GAGCGTAGGG CCAGCGGGCG CCGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540499.1
CDS	21..7850
Translation	MRIWPSGGPVGPNRAGPGPATNARREGGAARRASGTASAAGAQALPPPMAQVPGEVDNMEGPAVSNNNNPSARWESPDRGWDREPPAAANAAASLFECSRIKALADEREAVQKKTFTKWVNSHLARVGCHIGDLYADLRDGFVLTRLLEVLSGEQLPRPTRGRMRIHSLENVDKALQFLKEQRVHLENVGSHDIVDGNHRLTLGLVWTIILRFQIQVIKIETEDNRETRSAKDALLLWCQMKTAGYPEVNIQNFTTSWRDGLAFNALIHRHRPDLVDLSKLTKSNANYNLQRAFRTAEQHLGLARLLDPEDVNMEAPDEKSIITYVVSFYHYFSKMKALAVEGKRIGKVLDQVLEVDKIIERYEELAAELLAWIHRTVDLISNQKFANSLSGVQQQLQAFTAYCTLEKPVKFQEKGNLEVLLFSIQSKLRAHNRRLFVPREGCGIWDIDKAWGELEKAEHEREAALRAELIRQEKLELLAQRFDHKVAMRESWLNENQRLVSQDNFGYELPAVEAAMKKHEAIEADIAAYEERVQGVAELAQALAAEGYYDARRVAAQRDSVLRQWALLTGLVGARRTRLEQNLALQKVFQEMVYMVDWMEEMQTQLLSRECGQHLVEADDLLQKHGLLEGDIAAQSERVEALNAAALRFSQLQGYQPCDPQVICNRVNHVHGCLSELQEQAARRRAELEASRSLWALLQELEEAESWARDKERLLEATSGSGGAAGTAGGAHDLSSTARLLAQHKILQGELGGRRALLQQALRRGEELAAAGGSVGPGAEPLHLAGLAERAASARRRWQRLEEAAARRERRLQEARALHQFGADLDGLLDWLRDAYRLAAAGDFGHDEASSRRLARQHRALTGEVEAHRGPVGGLRRQLATLGGASGAGPLVVALQVRVVEAEQLFAEVTEVAALRRQWLRDALAVYRMFGEVHACELWIGEKEQWLLAMRVPDSLDDVEVVQHRFESLDQEMNSLMGRVLDVNQTVQELVEGGHPSSDEVRSCQDHLNSRWNRIVELVEQRKEEMSAVLLVENHVLEVAEVRAQVREKRRAVESAPRAGGALQWRLSGLEAALQALEPRQAALLEEAALLAERFPAQATRLHQGAEELGAEWGALAGAAQACGEAVAAAGRLQRFLRDLDTFLDWLVRAQEAAGAVEGPLPRSLEEADGLLARHAALKEEVDQREEDYARIVAASEALLASEGAELGPGLALDEWLPHLEVGWHKLLGLWEERREALVQAHVYQLFLRDLCQALAVLRNQEVALSGAELPCTVESVEEAMKRHRDFLTTMELNQQKMQVAVQAAESLLRQGNAYGEQAQEAVARLLEKSQENQLRAQQWMQKLLDQLVLQHFLRDCHELDGWIHEKMLMARDGTREDSHKLHKRWLRHQAFMAELAQNKEWLEKIEREGQQLMQEKPELAASVRKKLGEIRQCWAELESTTQAKARQLFEASKADQLVQSFAELDKRLLHMESQLQDVDPGGDLATVNSQLKKLQSMESQVEEWCREVGELQAQTAALPLEQASKELVGERQSAVGERLVRLLEPLQERRRLLLASKELHQVAHDLDDELAWVQERLPLAMQTERGTGLQAVQQHIKKNQGLRREIQAHGPRLEEVLERAGVLASLRSPEAEAVRRGQEQLQSAWTGLREAAERRQQTLDAAFQVEQYYFDVAEVEAWLGEQELLMMSEDKGKDEQSTLQLLKKHLQLEQGVENYEESIAQLSRQCRALLEMGHPDSEQISRRQSQVDRLYVALKELGEERRVSLEQQYWLYQLSRQVDELEHWIAEKEVVAGSPELGQDFEHVSVLQEKFSEFASETGTAGRERLAAVNQMVDELIECGHTAAATMAEWKDGLNEAWAELLELMGTRAQLLAASRELHKFFSDARELQGQIEEKRRRLPRLTAPPEPRPSASSMQRTLRAFEHDLQLLVSQVRQLQEGAAQLRTVYAGEHAEAIASREQEVLQGWKELLAACEDARLHVSSTADALRFHSQARDLLSWMDGIAGQIGAADKPRDVSSVEVLMNYHQGLKTELEARVPELATCQELGRSLLLNKSAMADEIQAQLDKLGSRKEEVSEKWDRHWEWLQQMLEVHQFAQEAVVADAWLTAQEPLLQSRELGSSVDEVEQLIRRHEAFRKAAAAWEERFSSLRRLTTIEKLKAEQSKQPPTPLLGRKFFGDPTELAAKAAPLLRPGGYDRGLEPLARRASDTLSAEVRTRVGYVRQELKPERLQPRIDRLPETSGKVEPAAPTAAALDTTDTPGTPAATELVRPRSERQELADRAEELPRRRRSERQESVDQPEETARRRRPERQESADHEGPHSLTLGRYEQMERRRERRERRIERQESSEQETPTRGELVKGKATLADIVEQLQEKEAGPGIPAGVPSLPQPRELPPGRLPNGLEPPERTPRPDRPRARDRPKPRRRPRPREGGEGGGSRRSRSAPAQGGSAPAPPPPPTHTVQHEGFLLRKRELDANRKSSNRSWVSLYCVLSKGELGFYKDSKGPASGGTHGGEPLLSLHKATSEVASDYKKKKHVFKLQTQDGSEFLLQAKDEEEMNGWLEAVANSVAEHAEIARWGQTLPTTSSTDEGNPKREGGERRASGRRK

Target ORF information:

RefSeq Version	XM_006540436.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540436.2

ORF Insert Sequence:

ATGCGGATCT GGCCGAGCGG CGGGCCGGTG GGGCCGAACA GAGCCGGGCC CGGCCCGGCG 
ACGAACGCCC GCAGGGAGGG CGGTGCTGCG CGGCGAGCAT CCGGGACCGC ATCGGCGGCC 
GGCGCCCAGG CCCTGCCTCC CCCAATGGCA CAAGTACCAG GGGAGGTGGA CAACATGGAG 
GGCCCAGCAG TCTCCAACAA CAACAACCCT TCAGCTCGCT GGGAGAGCCC AGATCGGGGC 
TGGGATCGGG AACCTCCCGC AGCCGCCAAT GCTGCGGCTT CACTCTTTGA GTGCTCTCGG 
ATCAAGGCTT TAGCAGATGA GCGAGAGGCT GTGCAGAAGA AAACCTTCAC CAAGTGGGTG 
AATTCGCACC TTGCCCGCGT GGGCTGCCAC ATTGGAGACC TGTATGCAGA CCTTCGGGAC 
GGGTTTGTCC TCACCCGGCT TCTGGAAGTG CTGTCGGGGG AGCAGCTGCC GAGGCCCACT 
CGCGGCCGCA TGCGGATCCA CTCGCTGGAG AACGTGGACA AGGCTCTGCA GTTCCTGAAG 
GAGCAGCGTG TGCATCTGGA GAACGTGGGG TCACATGACA TCGTGGATGG GAACCACCGA 
CTGACGCTGG GGCTGGTCTG GACCATCATC CTGCGCTTCC AGATTCAAGT CATCAAGATT 
GAGACAGAAG ACAACAGAGA GACCCGCTCC GCCAAGGACG CTCTGCTCTT GTGGTGCCAG 
ATGAAAACAG CCGGTTACCC TGAGGTAAAC ATCCAGAATT TCACCACCAG CTGGAGAGAT 
GGCCTGGCCT TCAATGCCCT CATTCACCGT CACAGGCCTG ATCTTGTGGA CCTCAGCAAA 
CTTACCAAGT CCAACGCCAA CTACAACCTG CAGAGAGCTT TCCGCACAGC CGAGCAGCAC 
CTGGGGCTAG CACGTCTGCT GGACCCCGAA GATGTGAACA TGGAGGCTCC AGATGAGAAG 
TCCATTATCA CCTATGTGGT CTCCTTCTAC CACTACTTCT CCAAGATGAA GGCTCTGGCG 
GTGGAGGGAA AACGGATTGG AAAGGTCCTG GACCAGGTGT TGGAGGTGGA CAAGATCATA 
GAACGCTATG AGGAGCTGGC GGCGGAGCTG TTGGCCTGGA TCCATCGCAC TGTGGACCTC 
ATTAGCAACC AAAAGTTTGC TAACTCACTG AGTGGGGTGC AGCAGCAGCT GCAGGCCTTC 
ACGGCTTACT GTACGCTGGA GAAGCCCGTC AAGTTTCAGG AGAAGGGGAA CCTGGAGGTG 
CTACTCTTCA GCATCCAAAG CAAACTGCGC GCCCACAACC GGCGCCTCTT TGTGCCTCGA 
GAAGGCTGTG GCATCTGGGA TATTGACAAG GCGTGGGGTG AGCTGGAGAA GGCAGAGCAT 
GAGCGAGAGG CGGCCCTCAG AGCTGAGCTC ATCCGGCAGG AGAAGTTGGA ACTCTTGGCT 
CAAAGATTTG ACCACAAGGT GGCCATGAGG GAGAGCTGGC TAAATGAGAA CCAGCGCCTG 
GTCTCCCAGG ACAACTTCGG ATATGAGCTG CCGGCAGTGG AGGCAGCCAT GAAGAAGCAC 
GAAGCCATTG AGGCAGACAT TGCAGCCTAC GAGGAGCGGG TTCAGGGTGT GGCGGAGTTA 
GCCCAGGCGT TGGCAGCTGA AGGCTACTAT GATGCCCGGC GGGTGGCTGC CCAGCGTGAC 
AGTGTCCTTC GCCAGTGGGC CCTGCTGACC GGGCTGGTAG GTGCCCGGCG GACCCGGCTT 
GAGCAGAATC TGGCCCTGCA GAAGGTCTTC CAGGAGATGG TCTACATGGT GGACTGGATG 
GAGGAGATGC AGACTCAGCT GCTGTCTCGG GAATGTGGGC AGCACCTGGT GGAGGCCGAT 
GACCTGCTGC AGAAGCACGG GCTGCTGGAG GGGGACATCG CAGCCCAGAG TGAGCGAGTG 
GAGGCCCTCA ATGCTGCAGC CCTGAGATTC TCCCAGCTAC AGGGCTATCA GCCCTGCGAC 
CCTCAGGTCA TTTGCAACCG TGTGAACCAC GTGCATGGCT GTCTGTCGGA GCTGCAGGAG 
CAGGCAGCAC GGCGGCGCGC AGAGCTGGAA GCTTCTCGGA GCCTGTGGGC GCTGCTGCAG 
GAGCTGGAGG AGGCGGAGAG TTGGGCGCGG GACAAGGAGC GCCTCCTAGA GGCCACGAGC 
GGCAGTGGTG GCGCGGCGGG CACGGCGGGC GGCGCGCACG ACCTGTCCAG CACCGCTCGC 
CTGCTGGCGC AGCATAAGAT CCTGCAGGGC GAGCTGGGCG GCCGGCGGGC GCTGCTGCAG 
CAGGCGCTGC GACGCGGGGA GGAGTTGGCT GCGGCGGGCG GCTCCGTGGG CCCGGGCGCC 
GAGCCTTTGC ATTTGGCTGG CCTGGCGGAG CGCGCGGCTA GCGCTCGGCG CCGCTGGCAG 
CGGCTGGAAG AGGCGGCTGC GCGCCGGGAA CGGCGGCTGC AGGAGGCGCG GGCGCTGCAC 
CAATTTGGCG CCGACCTGGA CGGGCTGCTG GACTGGCTTC GTGACGCGTA TCGTCTAGCA 
GCCGCTGGAG ATTTTGGTCA CGACGAAGCG TCCAGCCGTC GCCTGGCGCG CCAGCACCGC 
GCTCTCACCG GGGAGGTGGA AGCGCACCGT GGCCCTGTGG GTGGCCTGAG GCGCCAGCTG 
GCGACACTGG GAGGAGCCAG TGGCGCAGGG CCGCTGGTGG TGGCTCTGCA GGTGCGCGTG 
GTGGAAGCCG AACAGTTGTT CGCCGAGGTG ACCGAGGTGG CCGCGCTGCG ACGACAGTGG 
CTTCGGGACG CGCTCGCCGT GTACCGCATG TTCGGCGAGG TACATGCCTG CGAGTTGTGG 
ATCGGGGAGA AAGAGCAGTG GCTGCTGGCC ATGCGTGTGC CCGATTCCCT CGACGACGTT 
GAGGTGGTGC AGCATCGATT TGAAAGTCTT GACCAGGAAA TGAACAGCCT GATGGGCCGA 
GTCCTGGATG TGAACCAAAC AGTTCAGGAG TTGGTAGAAG GAGGTCATCC CAGCTCCGAT 
GAGGTGCGCT CCTGTCAGGA CCATCTCAAC AGCAGGTGGA ACCGCATCGT GGAGTTGGTG 
GAACAGCGCA AAGAGGAGAT GAGCGCGGTG CTGCTGGTGG AGAACCACGT GCTGGAGGTG 
GCCGAGGTGC GCGCCCAAGT GCGCGAGAAG CGACGGGCAG TGGAGAGCGC GCCCCGGGCG 
GGGGGAGCCC TGCAATGGCG CCTGAGTGGC CTGGAGGCGG CTCTACAGGC GCTAGAGCCG 
CGACAGGCGG CGCTGCTGGA GGAGGCGGCG CTGCTGGCCG AACGCTTCCC CGCACAGGCG 
ACGCGCCTGC ACCAGGGCGC CGAGGAGCTA GGAGCCGAGT GGGGCGCACT GGCCGGAGCG 
GCTCAGGCCT GCGGCGAGGC GGTTGCGGCG GCAGGTCGCC TGCAGCGTTT CCTGCGCGAT 
TTGGATACTT TCCTGGACTG GCTGGTGCGG GCGCAGGAGG CAGCAGGCGC CGTCGAGGGG 
CCGCTGCCTC GCAGCCTGGA GGAGGCGGAT GGGCTGCTGG CGCGCCACGC CGCGCTCAAG 
GAGGAGGTGG ATCAGCGTGA GGAGGACTAC GCGCGCATCG TGGCTGCCAG TGAAGCGCTG 
CTAGCGTCCG AGGGCGCGGA GCTGGGCCCC GGTCTGGCGC TAGACGAGTG GCTGCCGCAC 
CTCGAGGTTG GCTGGCACAA ACTGCTCGGC TTGTGGGAGG AGCGCAGGGA GGCGCTGGTC 
CAAGCGCACG TCTATCAACT CTTCCTGAGA GACCTGTGCC AGGCGCTTGC GGTGCTGCGC 
AACCAGGAGG TGGCGCTGTC TGGCGCTGAA CTCCCTTGCA CAGTGGAGTC GGTAGAAGAG 
GCGATGAAAA GGCACCGGGA TTTTCTCACC ACGATGGAGC TGAACCAGCA AAAGATGCAG 
GTGGCGGTTC AAGCTGCAGA GAGCCTGCTG CGGCAAGGCA ACGCCTACGG GGAGCAGGCG 
CAGGAGGCAG TGGCTCGGCT GCTAGAGAAG AGCCAAGAAA ATCAGCTGAG GGCCCAGCAG 
TGGATGCAAA AACTACTGGA CCAGCTTGTG CTGCAGCACT TTCTTAGAGA TTGTCATGAG 
CTGGATGGCT GGATCCATGA GAAGATGCTG ATGGCTCGTG ATGGCACCCG GGAAGACAGC 
CACAAGCTGC ACAAGAGATG GCTCCGGCAC CAGGCGTTCA TGGCTGAGCT GGCTCAGAAT 
AAGGAGTGGC TGGAGAAGAT AGAAAGGGAG GGCCAGCAAC TGATGCAGGA GAAACCGGAG 
CTGGCAGCCT CTGTGCGCAA GAAATTAGGT GAGATCCGGC AGTGCTGGGC GGAGCTGGAG 
AGCACCACAC AGGCCAAGGC GCGCCAGCTC TTCGAGGCCA GCAAGGCGGA CCAGCTAGTG 
CAGAGCTTCG CGGAGCTGGA CAAGCGGCTG CTTCACATGG AAAGCCAGCT GCAAGACGTA 
GACCCCGGTG GGGACCTGGC CACTGTCAAC AGCCAGCTCA AGAAGTTACA GTCCATGGAG 
TCTCAGGTGG AGGAGTGGTG CCGAGAGGTG GGCGAGCTTC AGGCGCAGAC GGCGGCGCTT 
CCGCTGGAGC AGGCGAGCAA GGAGTTGGTG GGGGAGCGGC AGAGTGCGGT GGGCGAGCGC 
CTGGTGCGCC TGCTGGAGCC TTTGCAGGAG CGACGCCGCT TGTTGCTGGC ATCCAAGGAG 
CTGCATCAGG TGGCCCACGA CCTGGACGAT GAACTGGCTT GGGTTCAAGA GCGGCTGCCA 
CTGGCCATGC AGACAGAGCG AGGCACTGGC TTGCAGGCTG TCCAGCAGCA CATCAAAAAG 
AACCAGGGCC TGCGGCGGGA GATCCAGGCT CACGGGCCGC GCTTGGAGGA GGTGCTGGAG 
CGCGCGGGCG TGCTGGCCTC GCTGCGCAGC CCAGAGGCCG AAGCGGTGCG CCGTGGCCAA 
GAGCAGCTGC AGAGCGCCTG GACCGGGCTG CGGGAAGCGG CCGAACGGCG GCAGCAGACG 
CTGGACGCCG CCTTCCAGGT GGAACAGTAC TACTTTGACG TGGCTGAGGT GGAGGCATGG 
CTGGGCGAGC AGGAGCTGCT CATGATGAGT GAGGACAAGG GCAAGGATGA ACAGAGCACC 
CTGCAGCTGC TCAAGAAGCA TTTACAACTG GAACAGGGCG TGGAGAACTA TGAGGAAAGC 
ATCGCCCAGC TGTCACGCCA GTGCCGGGCG CTACTGGAAA TGGGACACCC AGACAGTGAG 
CAAATCAGCC GCCGACAGTC CCAAGTGGAT CGCCTCTATG TGGCGCTCAA GGAGCTGGGC 
GAGGAACGCA GGGTGAGCCT GGAACAGCAG TACTGGCTCT ACCAGCTCAG CCGCCAGGTG 
GATGAGCTGG AACACTGGAT AGCCGAGAAG GAGGTGGTAG CTGGCTCCCC AGAGCTGGGC 
CAGGACTTCG AACACGTGTC GGTGCTACAG GAGAAATTCT CAGAGTTTGC CAGTGAGACA 
GGAACCGCAG GGCGGGAGCG GCTGGCGGCG GTCAACCAGA TGGTGGACGA GCTGATTGAG 
TGTGGTCACA CAGCAGCGGC CACCATGGCT GAGTGGAAGG ACGGGCTGAA CGAGGCCTGG 
GCTGAGCTGC TGGAACTCAT GGGCACTCGG GCCCAGCTGC TCGCTGCCTC TCGGGAGCTG 
CATAAGTTCT TCAGCGATGC CCGGGAGCTT CAAGGGCAGA TTGAGGAGAA GCGGAGGAGG 
CTGCCCCGCC TGACGGCACC ACCTGAACCC AGACCCAGTG CCAGCTCCAT GCAGCGGACC 
CTGCGAGCCT TTGAGCACGA CCTGCAGCTC CTCGTGTCCC AGGTACGGCA GCTGCAGGAA 
GGGGCGGCCC AGCTGCGGAC GGTGTACGCT GGCGAGCACG CCGAGGCCAT CGCCAGCCGC 
GAGCAGGAGG TGTTGCAGGG CTGGAAGGAG CTGCTAGCAG CCTGTGAAGA TGCTCGTCTG 
CACGTCAGCT CCACGGCCGA CGCCCTGCGC TTCCACAGCC AGGCCCGCGA CCTGCTCTCC 
TGGATGGACG GCATCGCGGG CCAGATCGGG GCAGCTGACA AACCCAGGGA TGTGTCATCG 
GTGGAGGTGC TTATGAACTA CCACCAGGGC CTGAAGACAG AGTTGGAGGC GCGCGTGCCT 
GAGCTGGCAA CCTGCCAGGA GCTGGGGCGG TCTCTATTAC TCAACAAAAG CGCCATGGCT 
GATGAGATCC AGGCCCAGCT GGACAAGCTG GGAAGCCGGA AGGAGGAGGT GTCGGAGAAG 
TGGGACCGTC ACTGGGAGTG GCTGCAGCAG ATGCTGGAGG TACACCAGTT TGCTCAGGAG 
GCCGTGGTGG CTGACGCCTG GCTGACAGCT CAGGAACCGC TCCTGCAGAG CCGGGAGCTG 
GGCAGCAGTG TGGATGAGGT GGAGCAGCTT ATCCGGCGAC ACGAGGCCTT CCGAAAAGCA 
GCTGCGGCCT GGGAGGAGAG GTTCAGCTCT CTGCGGCGCT TGACCACGAT CGAGAAACTC 
AAGGCAGAAC AGAGTAAGCA GCCACCCACC CCCTTACTGG GGCGCAAGTT CTTTGGGGAC 
CCCACAGAGC TGGCGGCCAA GGCAGCGCCT TTGCTGCGTC CAGGGGGCTA TGACAGGGGC 
CTGGAGCCTT TGGCCCGCAG GGCCTCGGAC ACGCTGTCAG CGGAGGTGCG GACCCGGGTT 
GGGTATGTGC GCCAGGAGCT CAAGCCCGAG CGTCTCCAGC CGCGCATTGA CCGGCTGCCA 
GAGACCTCGG GGAAGGTAGA ACCCGCGGCC CCGACAGCCG CAGCGTTGGA CACGACGGAC 
ACCCCTGGGA CTCCGGCGGC GACGGAGTTG GTCCGGCCCC GGTCGGAGCG CCAGGAGTTA 
GCGGATCGTG CGGAGGAGCT GCCACGGAGA CGAAGATCAG AACGCCAGGA GTCCGTGGAT 
CAACCAGAGG AGACAGCGCG GAGGCGGCGG CCTGAGCGCC AGGAGTCCGC GGACCACGAG 
GGGCCGCACA GCCTTACTCT AGGTCGCTAC GAGCAGATGG AGAGACGGCG GGAGCGGAGG 
GAGCGGCGGA TAGAACGACA GGAGTCTAGC GAACAGGAGA CTCCCACCAG GGGAGAGCTG 
GTCAAAGGGA AGGCCACTCT GGCTGATATT GTGGAGCAAC TTCAGGAGAA AGAGGCAGGC 
CCAGGGATCC CTGCTGGGGT GCCGTCGCTG CCTCAGCCTC GCGAGCTTCC CCCTGGTCGC 
CTGCCCAACG GGCTTGAGCC GCCCGAGCGG ACACCTCGGC CCGATCGGCC GCGGGCACGG 
GACCGGCCCA AGCCGCGCCG GCGGCCACGG CCTCGAGAGG GTGGTGAGGG CGGGGGAAGC 
CGGCGCTCGC GCTCCGCCCC TGCCCAGGGA GGCTCCGCCC CCGCGCCGCC ACCTCCGCCC 
ACTCACACAG TGCAGCACGA GGGCTTCCTG CTGCGCAAGC GCGAGCTCGA CGCTAACCGC 
AAGTCGTCCA ACCGGTCATG GGTGAGCCTG TACTGTGTGC TCAGCAAGGG GGAGCTGGGC 
TTCTACAAGG ACTCCAAAGG CCCAGCATCA GGGGGCACGC ATGGTGGGGA ACCACTGCTC 
AGCCTGCACA AAGCCACCAG CGAGGTGGCT AGTGACTACA AGAAAAAGAA GCATGTCTTC 
AAGCTCCAGA CCCAGGATGG CAGTGAGTTC TTGCTCCAGG CTAAAGATGA GGAGGAGATG 
AACGGCTGGC TGGAGGCTGT GGCTAACTCC GTGGCAGAAC ACGCCGAGAT CGCCCGATGG 
GGTCAGACAC TACCTACCAC ATCATCCACA GATGAGGGCA ACCCCAAGCG TGAGGGCGGG 
GAGCGTAGGG CCAGCGGGCG CCGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn4 ( XM_006540437.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540500.1 Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant X2, mRNA.

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CloneID	OMu38546
Clone ID Related Accession (Same CDS sequence)	XM_006540437.2
Accession Version	XM_006540437.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006540437.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTGG GCGACACCCG GTTTCATTCC TTGTGTTGCA ATGCCCTGCC TCCCCCAATG 
GCACAAGTAC CAGGGGAGGT GGACAACATG GAGGGCCCAG CAGTCTCCAA CAACAACAAC 
CCTTCAGCTC GCTGGGAGAG CCCAGATCGG GGCTGGGATC GGGAACCTCC CGCAGCCGCC 
AATGCTGCGG CTTCACTCTT TGAGTGCTCT CGGATCAAGG CTTTAGCAGA TGAGCGAGAG 
GCTGTGCAGA AGAAAACCTT CACCAAGTGG GTGAATTCGC ACCTTGCCCG CGTGGGCTGC 
CACATTGGAG ACCTGTATGC AGACCTTCGG GACGGGTTTG TCCTCACCCG GCTTCTGGAA 
GTGCTGTCGG GGGAGCAGCT GCCGAGGCCC ACTCGCGGCC GCATGCGGAT CCACTCGCTG 
GAGAACGTGG ACAAGGCTCT GCAGTTCCTG AAGGAGCAGC GTGTGCATCT GGAGAACGTG 
GGGTCACATG ACATCGTGGA TGGGAACCAC CGACTGACGC TGGGGCTGGT CTGGACCATC 
ATCCTGCGCT TCCAGATTCA AGTCATCAAG ATTGAGACAG AAGACAACAG AGAGACCCGC 
TCCGCCAAGG ACGCTCTGCT CTTGTGGTGC CAGATGAAAA CAGCCGGTTA CCCTGAGGTA 
AACATCCAGA ATTTCACCAC CAGCTGGAGA GATGGCCTGG CCTTCAATGC CCTCATTCAC 
CGTCACAGGC CTGATCTTGT GGACCTCAGC AAACTTACCA AGTCCAACGC CAACTACAAC 
CTGCAGAGAG CTTTCCGCAC AGCCGAGCAG CACCTGGGGC TAGCACGTCT GCTGGACCCC 
GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATTA TCACCTATGT GGTCTCCTTC 
TACCACTACT TCTCCAAGAT GAAGGCTCTG GCGGTGGAGG GAAAACGGAT TGGAAAGGTC 
CTGGACCAGG TGTTGGAGGT GGACAAGATC ATAGAACGCT ATGAGGAGCT GGCGGCGGAG 
CTGTTGGCCT GGATCCATCG CACTGTGGAC CTCATTAGCA ACCAAAAGTT TGCTAACTCA 
CTGAGTGGGG TGCAGCAGCA GCTGCAGGCC TTCACGGCTT ACTGTACGCT GGAGAAGCCC 
GTCAAGTTTC AGGAGAAGGG GAACCTGGAG GTGCTACTCT TCAGCATCCA AAGCAAACTG 
CGCGCCCACA ACCGGCGCCT CTTTGTGCCT CGAGAAGGCT GTGGCATCTG GGATATTGAC 
AAGGCGTGGG GTGAGCTGGA GAAGGCAGAG CATGAGCGAG AGGCGGCCCT CAGAGCTGAG 
CTCATCCGGC AGGAGAAGTT GGAACTCTTG GCTCAAAGAT TTGACCACAA GGTGGCCATG 
AGGGAGAGCT GGCTAAATGA GAACCAGCGC CTGGTCTCCC AGGACAACTT CGGATATGAG 
CTGCCGGCAG TGGAGGCAGC CATGAAGAAG CACGAAGCCA TTGAGGCAGA CATTGCAGCC 
TACGAGGAGC GGGTTCAGGG TGTGGCGGAG TTAGCCCAGG CGTTGGCAGC TGAAGGCTAC 
TATGATGCCC GGCGGGTGGC TGCCCAGCGT GACAGTGTCC TTCGCCAGTG GGCCCTGCTG 
ACCGGGCTGG TAGGTGCCCG GCGGACCCGG CTTGAGCAGA ATCTGGCCCT GCAGAAGGTC 
TTCCAGGAGA TGGTCTACAT GGTGGACTGG ATGGAGGAGA TGCAGACTCA GCTGCTGTCT 
CGGGAATGTG GGCAGCACCT GGTGGAGGCC GATGACCTGC TGCAGAAGCA CGGGCTGCTG 
GAGGGGGACA TCGCAGCCCA GAGTGAGCGA GTGGAGGCCC TCAATGCTGC AGCCCTGAGA 
TTCTCCCAGC TACAGGGCTA TCAGCCCTGC GACCCTCAGG TCATTTGCAA CCGTGTGAAC 
CACGTGCATG GCTGTCTGTC GGAGCTGCAG GAGCAGGCAG CACGGCGGCG CGCAGAGCTG 
GAAGCTTCTC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCGGA GAGTTGGGCG 
CGGGACAAGG AGCGCCTCCT AGAGGCCACG AGCGGCAGTG GTGGCGCGGC GGGCACGGCG 
GGCGGCGCGC ACGACCTGTC CAGCACCGCT CGCCTGCTGG CGCAGCATAA GATCCTGCAG 
GGCGAGCTGG GCGGCCGGCG GGCGCTGCTG CAGCAGGCGC TGCGACGCGG GGAGGAGTTG 
GCTGCGGCGG GCGGCTCCGT GGGCCCGGGC GCCGAGCCTT TGCATTTGGC TGGCCTGGCG 
GAGCGCGCGG CTAGCGCTCG GCGCCGCTGG CAGCGGCTGG AAGAGGCGGC TGCGCGCCGG 
GAACGGCGGC TGCAGGAGGC GCGGGCGCTG CACCAATTTG GCGCCGACCT GGACGGGCTG 
CTGGACTGGC TTCGTGACGC GTATCGTCTA GCAGCCGCTG GAGATTTTGG TCACGACGAA 
GCGTCCAGCC GTCGCCTGGC GCGCCAGCAC CGCGCTCTCA CCGGGGAGGT GGAAGCGCAC 
CGTGGCCCTG TGGGTGGCCT GAGGCGCCAG CTGGCGACAC TGGGAGGAGC CAGTGGCGCA 
GGGCCGCTGG TGGTGGCTCT GCAGGTGCGC GTGGTGGAAG CCGAACAGTT GTTCGCCGAG 
GTGACCGAGG TGGCCGCGCT GCGACGACAG TGGCTTCGGG ACGCGCTCGC CGTGTACCGC 
ATGTTCGGCG AGGTACATGC CTGCGAGTTG TGGATCGGGG AGAAAGAGCA GTGGCTGCTG 
GCCATGCGTG TGCCCGATTC CCTCGACGAC GTTGAGGTGG TGCAGCATCG ATTTGAAAGT 
CTTGACCAGG AAATGAACAG CCTGATGGGC CGAGTCCTGG ATGTGAACCA AACAGTTCAG 
GAGTTGGTAG AAGGAGGTCA TCCCAGCTCC GATGAGGTGC GCTCCTGTCA GGACCATCTC 
AACAGCAGGT GGAACCGCAT CGTGGAGTTG GTGGAACAGC GCAAAGAGGA GATGAGCGCG 
GTGCTGCTGG TGGAGAACCA CGTGCTGGAG GTGGCCGAGG TGCGCGCCCA AGTGCGCGAG 
AAGCGACGGG CAGTGGAGAG CGCGCCCCGG GCGGGGGGAG CCCTGCAATG GCGCCTGAGT 
GGCCTGGAGG CGGCTCTACA GGCGCTAGAG CCGCGACAGG CGGCGCTGCT GGAGGAGGCG 
GCGCTGCTGG CCGAACGCTT CCCCGCACAG GCGACGCGCC TGCACCAGGG CGCCGAGGAG 
CTAGGAGCCG AGTGGGGCGC ACTGGCCGGA GCGGCTCAGG CCTGCGGCGA GGCGGTTGCG 
GCGGCAGGTC GCCTGCAGCG TTTCCTGCGC GATTTGGATA CTTTCCTGGA CTGGCTGGTG 
CGGGCGCAGG AGGCAGCAGG CGCCGTCGAG GGGCCGCTGC CTCGCAGCCT GGAGGAGGCG 
GATGGGCTGC TGGCGCGCCA CGCCGCGCTC AAGGAGGAGG TGGATCAGCG TGAGGAGGAC 
TACGCGCGCA TCGTGGCTGC CAGTGAAGCG CTGCTAGCGT CCGAGGGCGC GGAGCTGGGC 
CCCGGTCTGG CGCTAGACGA GTGGCTGCCG CACCTCGAGG TTGGCTGGCA CAAACTGCTC 
GGCTTGTGGG AGGAGCGCAG GGAGGCGCTG GTCCAAGCGC ACGTCTATCA ACTCTTCCTG 
AGAGACCTGT GCCAGGCGCT TGCGGTGCTG CGCAACCAGG AGGTGGCGCT GTCTGGCGCT 
GAACTCCCTT GCACAGTGGA GTCGGTAGAA GAGGCGATGA AAAGGCACCG GGATTTTCTC 
ACCACGATGG AGCTGAACCA GCAAAAGATG CAGGTGGCGG TTCAAGCTGC AGAGAGCCTG 
CTGCGGCAAG GCAACGCCTA CGGGGAGCAG GCGCAGGAGG CAGTGGCTCG GCTGCTAGAG 
AAGAGCCAAG AAAATCAGCT GAGGGCCCAG CAGTGGATGC AAAAACTACT GGACCAGCTT 
GTGCTGCAGC ACTTTCTTAG AGATTGTCAT GAGCTGGATG GCTGGATCCA TGAGAAGATG 
CTGATGGCTC GTGATGGCAC CCGGGAAGAC AGCCACAAGC TGCACAAGAG ATGGCTCCGG 
CACCAGGCGT TCATGGCTGA GCTGGCTCAG AATAAGGAGT GGCTGGAGAA GATAGAAAGG 
GAGGGCCAGC AACTGATGCA GGAGAAACCG GAGCTGGCAG CCTCTGTGCG CAAGAAATTA 
GGTGAGATCC GGCAGTGCTG GGCGGAGCTG GAGAGCACCA CACAGGCCAA GGCGCGCCAG 
CTCTTCGAGG CCAGCAAGGC GGACCAGCTA GTGCAGAGCT TCGCGGAGCT GGACAAGCGG 
CTGCTTCACA TGGAAAGCCA GCTGCAAGAC GTAGACCCCG GTGGGGACCT GGCCACTGTC 
AACAGCCAGC TCAAGAAGTT ACAGTCCATG GAGTCTCAGG TGGAGGAGTG GTGCCGAGAG 
GTGGGCGAGC TTCAGGCGCA GACGGCGGCG CTTCCGCTGG AGCAGGCGAG CAAGGAGTTG 
GTGGGGGAGC GGCAGAGTGC GGTGGGCGAG CGCCTGGTGC GCCTGCTGGA GCCTTTGCAG 
GAGCGACGCC GCTTGTTGCT GGCATCCAAG GAGCTGCATC AGGTGGCCCA CGACCTGGAC 
GATGAACTGG CTTGGGTTCA AGAGCGGCTG CCACTGGCCA TGCAGACAGA GCGAGGCACT 
GGCTTGCAGG CTGTCCAGCA GCACATCAAA AAGAACCAGG GCCTGCGGCG GGAGATCCAG 
GCTCACGGGC CGCGCTTGGA GGAGGTGCTG GAGCGCGCGG GCGTGCTGGC CTCGCTGCGC 
AGCCCAGAGG CCGAAGCGGT GCGCCGTGGC CAAGAGCAGC TGCAGAGCGC CTGGACCGGG 
CTGCGGGAAG CGGCCGAACG GCGGCAGCAG ACGCTGGACG CCGCCTTCCA GGTGGAACAG 
TACTACTTTG ACGTGGCTGA GGTGGAGGCA TGGCTGGGCG AGCAGGAGCT GCTCATGATG 
AGTGAGGACA AGGGCAAGGA TGAACAGAGC ACCCTGCAGC TGCTCAAGAA GCATTTACAA 
CTGGAACAGG GCGTGGAGAA CTATGAGGAA AGCATCGCCC AGCTGTCACG CCAGTGCCGG 
GCGCTACTGG AAATGGGACA CCCAGACAGT GAGCAAATCA GCCGCCGACA GTCCCAAGTG 
GATCGCCTCT ATGTGGCGCT CAAGGAGCTG GGCGAGGAAC GCAGGGTGAG CCTGGAACAG 
CAGTACTGGC TCTACCAGCT CAGCCGCCAG GTGGATGAGC TGGAACACTG GATAGCCGAG 
AAGGAGGTGG TAGCTGGCTC CCCAGAGCTG GGCCAGGACT TCGAACACGT GTCGGTGCTA 
CAGGAGAAAT TCTCAGAGTT TGCCAGTGAG ACAGGAACCG CAGGGCGGGA GCGGCTGGCG 
GCGGTCAACC AGATGGTGGA CGAGCTGATT GAGTGTGGTC ACACAGCAGC GGCCACCATG 
GCTGAGTGGA AGGACGGGCT GAACGAGGCC TGGGCTGAGC TGCTGGAACT CATGGGCACT 
CGGGCCCAGC TGCTCGCTGC CTCTCGGGAG CTGCATAAGT TCTTCAGCGA TGCCCGGGAG 
CTTCAAGGGC AGATTGAGGA GAAGCGGAGG AGGCTGCCCC GCCTGACGGC ACCACCTGAA 
CCCAGACCCA GTGCCAGCTC CATGCAGCGG ACCCTGCGAG CCTTTGAGCA CGACCTGCAG 
CTCCTCGTGT CCCAGGTACG GCAGCTGCAG GAAGGGGCGG CCCAGCTGCG GACGGTGTAC 
GCTGGCGAGC ACGCCGAGGC CATCGCCAGC CGCGAGCAGG AGGTGTTGCA GGGCTGGAAG 
GAGCTGCTAG CAGCCTGTGA AGATGCTCGT CTGCACGTCA GCTCCACGGC CGACGCCCTG 
CGCTTCCACA GCCAGGCCCG CGACCTGCTC TCCTGGATGG ACGGCATCGC GGGCCAGATC 
GGGGCAGCTG ACAAACCCAG GGATGTGTCA TCGGTGGAGG TGCTTATGAA CTACCACCAG 
GGCCTGAAGA CAGAGTTGGA GGCGCGCGTG CCTGAGCTGG CAACCTGCCA GGAGCTGGGG 
CGGTCTCTAT TACTCAACAA AAGCGCCATG GCTGATGAGA TCCAGGCCCA GCTGGACAAG 
CTGGGAAGCC GGAAGGAGGA GGTGTCGGAG AAGTGGGACC GTCACTGGGA GTGGCTGCAG 
CAGATGCTGG AGGTACACCA GTTTGCTCAG GAGGCCGTGG TGGCTGACGC CTGGCTGACA 
GCTCAGGAAC CGCTCCTGCA GAGCCGGGAG CTGGGCAGCA GTGTGGATGA GGTGGAGCAG 
CTTATCCGGC GACACGAGGC CTTCCGAAAA GCAGCTGCGG CCTGGGAGGA GAGGTTCAGC 
TCTCTGCGGC GCTTGACCAC GATCGAGAAA CTCAAGGCAG AACAGAGTAA GCAGCCACCC 
ACCCCCTTAC TGGGGCGCAA GTTCTTTGGG GACCCCACAG AGCTGGCGGC CAAGGCAGCG 
CCTTTGCTGC GTCCAGGGGG CTATGACAGG GGCCTGGAGC CTTTGGCCCG CAGGGCCTCG 
GACACGCTGT CAGCGGAGGT GCGGACCCGG GTTGGGTATG TGCGCCAGGA GCTCAAGCCC 
GAGCGTCTCC AGCCGCGCAT TGACCGGCTG CCAGAGACCT CGGGGAAGGT AGAACCCGCG 
GCCCCGACAG CCGCAGCGTT GGACACGACG GACACCCCTG GGACTCCGGC GGCGACGGAG 
TTGGTCCGGC CCCGGTCGGA GCGCCAGGAG TTAGCGGATC GTGCGGAGGA GCTGCCACGG 
AGACGAAGAT CAGAACGCCA GGAGTCCGTG GATCAACCAG AGGAGACAGC GCGGAGGCGG 
CGGCCTGAGC GCCAGGAGTC CGCGGACCAC GAGGGGCCGC ACAGCCTTAC TCTAGGTCGC 
TACGAGCAGA TGGAGAGACG GCGGGAGCGG AGGGAGCGGC GGATAGAACG ACAGGAGTCT 
AGCGAACAGG AGACTCCCAC CAGGGGAGAG CTGGTCAAAG GGAAGGCCAC TCTGGCTGAT 
ATTGTGGAGC AACTTCAGGA GAAAGAGGCA GGCCCAGGGA TCCCTGCTGG GGTGCCGTCG 
CTGCCTCAGC CTCGCGAGCT TCCCCCTGGT CGCCTGCCCA ACGGGCTTGA GCCGCCCGAG 
CGGACACCTC GGCCCGATCG GCCGCGGGCA CGGGACCGGC CCAAGCCGCG CCGGCGGCCA 
CGGCCTCGAG AGGGTGGTGA GGGCGGGGGA AGCCGGCGCT CGCGCTCCGC CCCTGCCCAG 
GGAGGCTCCG CCCCCGCGCC GCCACCTCCG CCCACTCACA CAGTGCAGCA CGAGGGCTTC 
CTGCTGCGCA AGCGCGAGCT CGACGCTAAC CGCAAGTCGT CCAACCGGTC ATGGGTGAGC 
CTGTACTGTG TGCTCAGCAA GGGGGAGCTG GGCTTCTACA AGGACTCCAA AGGCCCAGCA 
TCAGGGGGCA CGCATGGTGG GGAACCACTG CTCAGCCTGC ACAAAGCCAC CAGCGAGGTG 
GCTAGTGACT ACAAGAAAAA GAAGCATGTC TTCAAGCTCC AGACCCAGGA TGGCAGTGAG 
TTCTTGCTCC AGGCTAAAGA TGAGGAGGAG ATGAACGGCT GGCTGGAGGC TGTGGCTAAC 
TCCGTGGCAG AACACGCCGA GATCGCCCGA TGGGGTCAGA CACTACCTAC CACATCATCC 
ACAGATGAGG GCAACCCCAA GCGTGAGGGC GGGGAGCGTA GGGCCAGCGG GCGCCGGAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540500.1
CDS	126..7868
Translation	MVVGDTRFHSLCCNALPPPMAQVPGEVDNMEGPAVSNNNNPSARWESPDRGWDREPPAAANAAASLFECSRIKALADEREAVQKKTFTKWVNSHLARVGCHIGDLYADLRDGFVLTRLLEVLSGEQLPRPTRGRMRIHSLENVDKALQFLKEQRVHLENVGSHDIVDGNHRLTLGLVWTIILRFQIQVIKIETEDNRETRSAKDALLLWCQMKTAGYPEVNIQNFTTSWRDGLAFNALIHRHRPDLVDLSKLTKSNANYNLQRAFRTAEQHLGLARLLDPEDVNMEAPDEKSIITYVVSFYHYFSKMKALAVEGKRIGKVLDQVLEVDKIIERYEELAAELLAWIHRTVDLISNQKFANSLSGVQQQLQAFTAYCTLEKPVKFQEKGNLEVLLFSIQSKLRAHNRRLFVPREGCGIWDIDKAWGELEKAEHEREAALRAELIRQEKLELLAQRFDHKVAMRESWLNENQRLVSQDNFGYELPAVEAAMKKHEAIEADIAAYEERVQGVAELAQALAAEGYYDARRVAAQRDSVLRQWALLTGLVGARRTRLEQNLALQKVFQEMVYMVDWMEEMQTQLLSRECGQHLVEADDLLQKHGLLEGDIAAQSERVEALNAAALRFSQLQGYQPCDPQVICNRVNHVHGCLSELQEQAARRRAELEASRSLWALLQELEEAESWARDKERLLEATSGSGGAAGTAGGAHDLSSTARLLAQHKILQGELGGRRALLQQALRRGEELAAAGGSVGPGAEPLHLAGLAERAASARRRWQRLEEAAARRERRLQEARALHQFGADLDGLLDWLRDAYRLAAAGDFGHDEASSRRLARQHRALTGEVEAHRGPVGGLRRQLATLGGASGAGPLVVALQVRVVEAEQLFAEVTEVAALRRQWLRDALAVYRMFGEVHACELWIGEKEQWLLAMRVPDSLDDVEVVQHRFESLDQEMNSLMGRVLDVNQTVQELVEGGHPSSDEVRSCQDHLNSRWNRIVELVEQRKEEMSAVLLVENHVLEVAEVRAQVREKRRAVESAPRAGGALQWRLSGLEAALQALEPRQAALLEEAALLAERFPAQATRLHQGAEELGAEWGALAGAAQACGEAVAAAGRLQRFLRDLDTFLDWLVRAQEAAGAVEGPLPRSLEEADGLLARHAALKEEVDQREEDYARIVAASEALLASEGAELGPGLALDEWLPHLEVGWHKLLGLWEERREALVQAHVYQLFLRDLCQALAVLRNQEVALSGAELPCTVESVEEAMKRHRDFLTTMELNQQKMQVAVQAAESLLRQGNAYGEQAQEAVARLLEKSQENQLRAQQWMQKLLDQLVLQHFLRDCHELDGWIHEKMLMARDGTREDSHKLHKRWLRHQAFMAELAQNKEWLEKIEREGQQLMQEKPELAASVRKKLGEIRQCWAELESTTQAKARQLFEASKADQLVQSFAELDKRLLHMESQLQDVDPGGDLATVNSQLKKLQSMESQVEEWCREVGELQAQTAALPLEQASKELVGERQSAVGERLVRLLEPLQERRRLLLASKELHQVAHDLDDELAWVQERLPLAMQTERGTGLQAVQQHIKKNQGLRREIQAHGPRLEEVLERAGVLASLRSPEAEAVRRGQEQLQSAWTGLREAAERRQQTLDAAFQVEQYYFDVAEVEAWLGEQELLMMSEDKGKDEQSTLQLLKKHLQLEQGVENYEESIAQLSRQCRALLEMGHPDSEQISRRQSQVDRLYVALKELGEERRVSLEQQYWLYQLSRQVDELEHWIAEKEVVAGSPELGQDFEHVSVLQEKFSEFASETGTAGRERLAAVNQMVDELIECGHTAAATMAEWKDGLNEAWAELLELMGTRAQLLAASRELHKFFSDARELQGQIEEKRRRLPRLTAPPEPRPSASSMQRTLRAFEHDLQLLVSQVRQLQEGAAQLRTVYAGEHAEAIASREQEVLQGWKELLAACEDARLHVSSTADALRFHSQARDLLSWMDGIAGQIGAADKPRDVSSVEVLMNYHQGLKTELEARVPELATCQELGRSLLLNKSAMADEIQAQLDKLGSRKEEVSEKWDRHWEWLQQMLEVHQFAQEAVVADAWLTAQEPLLQSRELGSSVDEVEQLIRRHEAFRKAAAAWEERFSSLRRLTTIEKLKAEQSKQPPTPLLGRKFFGDPTELAAKAAPLLRPGGYDRGLEPLARRASDTLSAEVRTRVGYVRQELKPERLQPRIDRLPETSGKVEPAAPTAAALDTTDTPGTPAATELVRPRSERQELADRAEELPRRRRSERQESVDQPEETARRRRPERQESADHEGPHSLTLGRYEQMERRRERRERRIERQESSEQETPTRGELVKGKATLADIVEQLQEKEAGPGIPAGVPSLPQPRELPPGRLPNGLEPPERTPRPDRPRARDRPKPRRRPRPREGGEGGGSRRSRSAPAQGGSAPAPPPPPTHTVQHEGFLLRKRELDANRKSSNRSWVSLYCVLSKGELGFYKDSKGPASGGTHGGEPLLSLHKATSEVASDYKKKKHVFKLQTQDGSEFLLQAKDEEEMNGWLEAVANSVAEHAEIARWGQTLPTTSSTDEGNPKREGGERRASGRRK

Target ORF information:

RefSeq Version	XM_006540437.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 4 (Sptbn4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540437.2

ORF Insert Sequence:

ATGGTTGTGG GCGACACCCG GTTTCATTCC TTGTGTTGCA ATGCCCTGCC TCCCCCAATG 
GCACAAGTAC CAGGGGAGGT GGACAACATG GAGGGCCCAG CAGTCTCCAA CAACAACAAC 
CCTTCAGCTC GCTGGGAGAG CCCAGATCGG GGCTGGGATC GGGAACCTCC CGCAGCCGCC 
AATGCTGCGG CTTCACTCTT TGAGTGCTCT CGGATCAAGG CTTTAGCAGA TGAGCGAGAG 
GCTGTGCAGA AGAAAACCTT CACCAAGTGG GTGAATTCGC ACCTTGCCCG CGTGGGCTGC 
CACATTGGAG ACCTGTATGC AGACCTTCGG GACGGGTTTG TCCTCACCCG GCTTCTGGAA 
GTGCTGTCGG GGGAGCAGCT GCCGAGGCCC ACTCGCGGCC GCATGCGGAT CCACTCGCTG 
GAGAACGTGG ACAAGGCTCT GCAGTTCCTG AAGGAGCAGC GTGTGCATCT GGAGAACGTG 
GGGTCACATG ACATCGTGGA TGGGAACCAC CGACTGACGC TGGGGCTGGT CTGGACCATC 
ATCCTGCGCT TCCAGATTCA AGTCATCAAG ATTGAGACAG AAGACAACAG AGAGACCCGC 
TCCGCCAAGG ACGCTCTGCT CTTGTGGTGC CAGATGAAAA CAGCCGGTTA CCCTGAGGTA 
AACATCCAGA ATTTCACCAC CAGCTGGAGA GATGGCCTGG CCTTCAATGC CCTCATTCAC 
CGTCACAGGC CTGATCTTGT GGACCTCAGC AAACTTACCA AGTCCAACGC CAACTACAAC 
CTGCAGAGAG CTTTCCGCAC AGCCGAGCAG CACCTGGGGC TAGCACGTCT GCTGGACCCC 
GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATTA TCACCTATGT GGTCTCCTTC 
TACCACTACT TCTCCAAGAT GAAGGCTCTG GCGGTGGAGG GAAAACGGAT TGGAAAGGTC 
CTGGACCAGG TGTTGGAGGT GGACAAGATC ATAGAACGCT ATGAGGAGCT GGCGGCGGAG 
CTGTTGGCCT GGATCCATCG CACTGTGGAC CTCATTAGCA ACCAAAAGTT TGCTAACTCA 
CTGAGTGGGG TGCAGCAGCA GCTGCAGGCC TTCACGGCTT ACTGTACGCT GGAGAAGCCC 
GTCAAGTTTC AGGAGAAGGG GAACCTGGAG GTGCTACTCT TCAGCATCCA AAGCAAACTG 
CGCGCCCACA ACCGGCGCCT CTTTGTGCCT CGAGAAGGCT GTGGCATCTG GGATATTGAC 
AAGGCGTGGG GTGAGCTGGA GAAGGCAGAG CATGAGCGAG AGGCGGCCCT CAGAGCTGAG 
CTCATCCGGC AGGAGAAGTT GGAACTCTTG GCTCAAAGAT TTGACCACAA GGTGGCCATG 
AGGGAGAGCT GGCTAAATGA GAACCAGCGC CTGGTCTCCC AGGACAACTT CGGATATGAG 
CTGCCGGCAG TGGAGGCAGC CATGAAGAAG CACGAAGCCA TTGAGGCAGA CATTGCAGCC 
TACGAGGAGC GGGTTCAGGG TGTGGCGGAG TTAGCCCAGG CGTTGGCAGC TGAAGGCTAC 
TATGATGCCC GGCGGGTGGC TGCCCAGCGT GACAGTGTCC TTCGCCAGTG GGCCCTGCTG 
ACCGGGCTGG TAGGTGCCCG GCGGACCCGG CTTGAGCAGA ATCTGGCCCT GCAGAAGGTC 
TTCCAGGAGA TGGTCTACAT GGTGGACTGG ATGGAGGAGA TGCAGACTCA GCTGCTGTCT 
CGGGAATGTG GGCAGCACCT GGTGGAGGCC GATGACCTGC TGCAGAAGCA CGGGCTGCTG 
GAGGGGGACA TCGCAGCCCA GAGTGAGCGA GTGGAGGCCC TCAATGCTGC AGCCCTGAGA 
TTCTCCCAGC TACAGGGCTA TCAGCCCTGC GACCCTCAGG TCATTTGCAA CCGTGTGAAC 
CACGTGCATG GCTGTCTGTC GGAGCTGCAG GAGCAGGCAG CACGGCGGCG CGCAGAGCTG 
GAAGCTTCTC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCGGA GAGTTGGGCG 
CGGGACAAGG AGCGCCTCCT AGAGGCCACG AGCGGCAGTG GTGGCGCGGC GGGCACGGCG 
GGCGGCGCGC ACGACCTGTC CAGCACCGCT CGCCTGCTGG CGCAGCATAA GATCCTGCAG 
GGCGAGCTGG GCGGCCGGCG GGCGCTGCTG CAGCAGGCGC TGCGACGCGG GGAGGAGTTG 
GCTGCGGCGG GCGGCTCCGT GGGCCCGGGC GCCGAGCCTT TGCATTTGGC TGGCCTGGCG 
GAGCGCGCGG CTAGCGCTCG GCGCCGCTGG CAGCGGCTGG AAGAGGCGGC TGCGCGCCGG 
GAACGGCGGC TGCAGGAGGC GCGGGCGCTG CACCAATTTG GCGCCGACCT GGACGGGCTG 
CTGGACTGGC TTCGTGACGC GTATCGTCTA GCAGCCGCTG GAGATTTTGG TCACGACGAA 
GCGTCCAGCC GTCGCCTGGC GCGCCAGCAC CGCGCTCTCA CCGGGGAGGT GGAAGCGCAC 
CGTGGCCCTG TGGGTGGCCT GAGGCGCCAG CTGGCGACAC TGGGAGGAGC CAGTGGCGCA 
GGGCCGCTGG TGGTGGCTCT GCAGGTGCGC GTGGTGGAAG CCGAACAGTT GTTCGCCGAG 
GTGACCGAGG TGGCCGCGCT GCGACGACAG TGGCTTCGGG ACGCGCTCGC CGTGTACCGC 
ATGTTCGGCG AGGTACATGC CTGCGAGTTG TGGATCGGGG AGAAAGAGCA GTGGCTGCTG 
GCCATGCGTG TGCCCGATTC CCTCGACGAC GTTGAGGTGG TGCAGCATCG ATTTGAAAGT 
CTTGACCAGG AAATGAACAG CCTGATGGGC CGAGTCCTGG ATGTGAACCA AACAGTTCAG 
GAGTTGGTAG AAGGAGGTCA TCCCAGCTCC GATGAGGTGC GCTCCTGTCA GGACCATCTC 
AACAGCAGGT GGAACCGCAT CGTGGAGTTG GTGGAACAGC GCAAAGAGGA GATGAGCGCG 
GTGCTGCTGG TGGAGAACCA CGTGCTGGAG GTGGCCGAGG TGCGCGCCCA AGTGCGCGAG 
AAGCGACGGG CAGTGGAGAG CGCGCCCCGG GCGGGGGGAG CCCTGCAATG GCGCCTGAGT 
GGCCTGGAGG CGGCTCTACA GGCGCTAGAG CCGCGACAGG CGGCGCTGCT GGAGGAGGCG 
GCGCTGCTGG CCGAACGCTT CCCCGCACAG GCGACGCGCC TGCACCAGGG CGCCGAGGAG 
CTAGGAGCCG AGTGGGGCGC ACTGGCCGGA GCGGCTCAGG CCTGCGGCGA GGCGGTTGCG 
GCGGCAGGTC GCCTGCAGCG TTTCCTGCGC GATTTGGATA CTTTCCTGGA CTGGCTGGTG 
CGGGCGCAGG AGGCAGCAGG CGCCGTCGAG GGGCCGCTGC CTCGCAGCCT GGAGGAGGCG 
GATGGGCTGC TGGCGCGCCA CGCCGCGCTC AAGGAGGAGG TGGATCAGCG TGAGGAGGAC 
TACGCGCGCA TCGTGGCTGC CAGTGAAGCG CTGCTAGCGT CCGAGGGCGC GGAGCTGGGC 
CCCGGTCTGG CGCTAGACGA GTGGCTGCCG CACCTCGAGG TTGGCTGGCA CAAACTGCTC 
GGCTTGTGGG AGGAGCGCAG GGAGGCGCTG GTCCAAGCGC ACGTCTATCA ACTCTTCCTG 
AGAGACCTGT GCCAGGCGCT TGCGGTGCTG CGCAACCAGG AGGTGGCGCT GTCTGGCGCT 
GAACTCCCTT GCACAGTGGA GTCGGTAGAA GAGGCGATGA AAAGGCACCG GGATTTTCTC 
ACCACGATGG AGCTGAACCA GCAAAAGATG CAGGTGGCGG TTCAAGCTGC AGAGAGCCTG 
CTGCGGCAAG GCAACGCCTA CGGGGAGCAG GCGCAGGAGG CAGTGGCTCG GCTGCTAGAG 
AAGAGCCAAG AAAATCAGCT GAGGGCCCAG CAGTGGATGC AAAAACTACT GGACCAGCTT 
GTGCTGCAGC ACTTTCTTAG AGATTGTCAT GAGCTGGATG GCTGGATCCA TGAGAAGATG 
CTGATGGCTC GTGATGGCAC CCGGGAAGAC AGCCACAAGC TGCACAAGAG ATGGCTCCGG 
CACCAGGCGT TCATGGCTGA GCTGGCTCAG AATAAGGAGT GGCTGGAGAA GATAGAAAGG 
GAGGGCCAGC AACTGATGCA GGAGAAACCG GAGCTGGCAG CCTCTGTGCG CAAGAAATTA 
GGTGAGATCC GGCAGTGCTG GGCGGAGCTG GAGAGCACCA CACAGGCCAA GGCGCGCCAG 
CTCTTCGAGG CCAGCAAGGC GGACCAGCTA GTGCAGAGCT TCGCGGAGCT GGACAAGCGG 
CTGCTTCACA TGGAAAGCCA GCTGCAAGAC GTAGACCCCG GTGGGGACCT GGCCACTGTC 
AACAGCCAGC TCAAGAAGTT ACAGTCCATG GAGTCTCAGG TGGAGGAGTG GTGCCGAGAG 
GTGGGCGAGC TTCAGGCGCA GACGGCGGCG CTTCCGCTGG AGCAGGCGAG CAAGGAGTTG 
GTGGGGGAGC GGCAGAGTGC GGTGGGCGAG CGCCTGGTGC GCCTGCTGGA GCCTTTGCAG 
GAGCGACGCC GCTTGTTGCT GGCATCCAAG GAGCTGCATC AGGTGGCCCA CGACCTGGAC 
GATGAACTGG CTTGGGTTCA AGAGCGGCTG CCACTGGCCA TGCAGACAGA GCGAGGCACT 
GGCTTGCAGG CTGTCCAGCA GCACATCAAA AAGAACCAGG GCCTGCGGCG GGAGATCCAG 
GCTCACGGGC CGCGCTTGGA GGAGGTGCTG GAGCGCGCGG GCGTGCTGGC CTCGCTGCGC 
AGCCCAGAGG CCGAAGCGGT GCGCCGTGGC CAAGAGCAGC TGCAGAGCGC CTGGACCGGG 
CTGCGGGAAG CGGCCGAACG GCGGCAGCAG ACGCTGGACG CCGCCTTCCA GGTGGAACAG 
TACTACTTTG ACGTGGCTGA GGTGGAGGCA TGGCTGGGCG AGCAGGAGCT GCTCATGATG 
AGTGAGGACA AGGGCAAGGA TGAACAGAGC ACCCTGCAGC TGCTCAAGAA GCATTTACAA 
CTGGAACAGG GCGTGGAGAA CTATGAGGAA AGCATCGCCC AGCTGTCACG CCAGTGCCGG 
GCGCTACTGG AAATGGGACA CCCAGACAGT GAGCAAATCA GCCGCCGACA GTCCCAAGTG 
GATCGCCTCT ATGTGGCGCT CAAGGAGCTG GGCGAGGAAC GCAGGGTGAG CCTGGAACAG 
CAGTACTGGC TCTACCAGCT CAGCCGCCAG GTGGATGAGC TGGAACACTG GATAGCCGAG 
AAGGAGGTGG TAGCTGGCTC CCCAGAGCTG GGCCAGGACT TCGAACACGT GTCGGTGCTA 
CAGGAGAAAT TCTCAGAGTT TGCCAGTGAG ACAGGAACCG CAGGGCGGGA GCGGCTGGCG 
GCGGTCAACC AGATGGTGGA CGAGCTGATT GAGTGTGGTC ACACAGCAGC GGCCACCATG 
GCTGAGTGGA AGGACGGGCT GAACGAGGCC TGGGCTGAGC TGCTGGAACT CATGGGCACT 
CGGGCCCAGC TGCTCGCTGC CTCTCGGGAG CTGCATAAGT TCTTCAGCGA TGCCCGGGAG 
CTTCAAGGGC AGATTGAGGA GAAGCGGAGG AGGCTGCCCC GCCTGACGGC ACCACCTGAA 
CCCAGACCCA GTGCCAGCTC CATGCAGCGG ACCCTGCGAG CCTTTGAGCA CGACCTGCAG 
CTCCTCGTGT CCCAGGTACG GCAGCTGCAG GAAGGGGCGG CCCAGCTGCG GACGGTGTAC 
GCTGGCGAGC ACGCCGAGGC CATCGCCAGC CGCGAGCAGG AGGTGTTGCA GGGCTGGAAG 
GAGCTGCTAG CAGCCTGTGA AGATGCTCGT CTGCACGTCA GCTCCACGGC CGACGCCCTG 
CGCTTCCACA GCCAGGCCCG CGACCTGCTC TCCTGGATGG ACGGCATCGC GGGCCAGATC 
GGGGCAGCTG ACAAACCCAG GGATGTGTCA TCGGTGGAGG TGCTTATGAA CTACCACCAG 
GGCCTGAAGA CAGAGTTGGA GGCGCGCGTG CCTGAGCTGG CAACCTGCCA GGAGCTGGGG 
CGGTCTCTAT TACTCAACAA AAGCGCCATG GCTGATGAGA TCCAGGCCCA GCTGGACAAG 
CTGGGAAGCC GGAAGGAGGA GGTGTCGGAG AAGTGGGACC GTCACTGGGA GTGGCTGCAG 
CAGATGCTGG AGGTACACCA GTTTGCTCAG GAGGCCGTGG TGGCTGACGC CTGGCTGACA 
GCTCAGGAAC CGCTCCTGCA GAGCCGGGAG CTGGGCAGCA GTGTGGATGA GGTGGAGCAG 
CTTATCCGGC GACACGAGGC CTTCCGAAAA GCAGCTGCGG CCTGGGAGGA GAGGTTCAGC 
TCTCTGCGGC GCTTGACCAC GATCGAGAAA CTCAAGGCAG AACAGAGTAA GCAGCCACCC 
ACCCCCTTAC TGGGGCGCAA GTTCTTTGGG GACCCCACAG AGCTGGCGGC CAAGGCAGCG 
CCTTTGCTGC GTCCAGGGGG CTATGACAGG GGCCTGGAGC CTTTGGCCCG CAGGGCCTCG 
GACACGCTGT CAGCGGAGGT GCGGACCCGG GTTGGGTATG TGCGCCAGGA GCTCAAGCCC 
GAGCGTCTCC AGCCGCGCAT TGACCGGCTG CCAGAGACCT CGGGGAAGGT AGAACCCGCG 
GCCCCGACAG CCGCAGCGTT GGACACGACG GACACCCCTG GGACTCCGGC GGCGACGGAG 
TTGGTCCGGC CCCGGTCGGA GCGCCAGGAG TTAGCGGATC GTGCGGAGGA GCTGCCACGG 
AGACGAAGAT CAGAACGCCA GGAGTCCGTG GATCAACCAG AGGAGACAGC GCGGAGGCGG 
CGGCCTGAGC GCCAGGAGTC CGCGGACCAC GAGGGGCCGC ACAGCCTTAC TCTAGGTCGC 
TACGAGCAGA TGGAGAGACG GCGGGAGCGG AGGGAGCGGC GGATAGAACG ACAGGAGTCT 
AGCGAACAGG AGACTCCCAC CAGGGGAGAG CTGGTCAAAG GGAAGGCCAC TCTGGCTGAT 
ATTGTGGAGC AACTTCAGGA GAAAGAGGCA GGCCCAGGGA TCCCTGCTGG GGTGCCGTCG 
CTGCCTCAGC CTCGCGAGCT TCCCCCTGGT CGCCTGCCCA ACGGGCTTGA GCCGCCCGAG 
CGGACACCTC GGCCCGATCG GCCGCGGGCA CGGGACCGGC CCAAGCCGCG CCGGCGGCCA 
CGGCCTCGAG AGGGTGGTGA GGGCGGGGGA AGCCGGCGCT CGCGCTCCGC CCCTGCCCAG 
GGAGGCTCCG CCCCCGCGCC GCCACCTCCG CCCACTCACA CAGTGCAGCA CGAGGGCTTC 
CTGCTGCGCA AGCGCGAGCT CGACGCTAAC CGCAAGTCGT CCAACCGGTC ATGGGTGAGC 
CTGTACTGTG TGCTCAGCAA GGGGGAGCTG GGCTTCTACA AGGACTCCAA AGGCCCAGCA 
TCAGGGGGCA CGCATGGTGG GGAACCACTG CTCAGCCTGC ACAAAGCCAC CAGCGAGGTG 
GCTAGTGACT ACAAGAAAAA GAAGCATGTC TTCAAGCTCC AGACCCAGGA TGGCAGTGAG 
TTCTTGCTCC AGGCTAAAGA TGAGGAGGAG ATGAACGGCT GGCTGGAGGC TGTGGCTAAC 
TCCGTGGCAG AACACGCCGA GATCGCCCGA TGGGGTCAGA CACTACCTAC CACATCATCC 
ACAGATGAGG GCAACCCCAA GCGTGAGGGC GGGGAGCGTA GGGCCAGCGG GCGCCGGAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Schwannomin-interacting Protein 1 Isoform IQCJ-SCHIP1 Is a Multipartner Ankyrin- and Spectrin-binding Protein Involved in the Organization of Nodes of Ranvier.
The Journal of biological chemistry292(6)2441-2456(2017 02)
Martin PM,Cifuentes-Diaz C,Devaux J,Garcia M,Bureau J,Thomasseau S,Klingler E,Girault JA,Goutebroze L

Postnatal Development of Spasticity Following Transgene Insertion in the Mouse ?IV Spectrin Gene (SPTBN4).
Journal of neuromuscular diseases4(2)159-164(2017)
Kichkin E,Visvanathan A,Lovicu FJ,Shu DY,Das SJ,Reddel SW,McCann EP,Zhang KY,Williams KL,Blair IP,Phillips WD

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Sptbn4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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