Slc25a12 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc25a12
Entrez Gene ID	78830
Full Name	solute carrier family 25 (mitochondrial carrier, Aralar), member 12
Synonyms	2610002D09Rik,AI839531,B230107K20Rik,BB129864
General protein information	Preferred Names solute carrier family 25 (mitochondrial carrier, Aralar), member 12 Names calcium-binding mitochondrial carrier protein Aralar1 mitochondrial aspartate glutamate carrier 1 solute carrier family 25 member 12
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 C2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006500420.1	XP_006500483.1	calcium-binding mitochondrial carrier protein Aralar1 isoform X2
NM_172436.4	NP_766024.1	calcium-binding mitochondrial carrier protein Aralar1
XM_030252254.1	XP_030108114.1	calcium-binding mitochondrial carrier protein Aralar1 isoform X2
NM_172436.3	NP_766024.1	calcium-binding mitochondrial carrier protein Aralar1
XM_011239853.2	XP_011238155.1	calcium-binding mitochondrial carrier protein Aralar1 isoform X1
XM_011239853.3	XP_011238155.1	calcium-binding mitochondrial carrier protein Aralar1 isoform X1

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	K02F3.2	NP_497274.2
	SLC25A12	XP_001085208.1
Canis lupus familiaris (dog)	SLC25A12	XP_005640369.1
Gallus gallus (chicken)	SLC25A12	XP_428938.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000958	XP_309148.5
Neurospora crassa	NCU01241	XP_961607.1
Homo sapiens (human)	SLC25A12	NP_003696.2
Pan troglodytes (chimpanzee)	SLC25A12	XP_003949966.1
Bos taurus (cattle)	SLC25A12	NP_001094664.1
Mus musculus (house mouse)	Slc25a12	NP_766024.1
Danio rerio (zebrafish)	slc25a12	NP_997947.1
Drosophila melanogaster (fruit fly)	aralar1	NP_001247368.1
Magnaporthe oryzae (rice blast fungus)	MGG_07066	XP_003715248.1

Gene Ontology
Bibliography

Related articles in PubMed

ARALAR/AGC1 deficiency, a neurodevelopmental disorder with severe impairment of neuronal mitochondrial respiration, does not produce a primary increase in brain lactate.
Juaristi I, Garc?a-Mart?n ML, Rodrigues TB, Satr?stegui J, Llorente-Folch I, Pardo B
Journal of neurochemistry142(1)132-139(2017 Jul)

L-Lactate-Mediated Neuroprotection against Glutamate-Induced Excitotoxicity Requires ARALAR/AGC1.
Llorente-Folch I, Rueda CB, P?rez-Li?bana I, Satr?stegui J, Pardo B
The Journal of neuroscience : the official journal of the Society for Neuroscience36(16)4443-56(2016 Apr)

Phototransduction Influences Metabolic Flux and Nucleotide Metabolism in Mouse Retina.
Du J, Rountree A, Cleghorn WM, Contreras L, Lindsay KJ, Sadilek M, Gu H, Djukovic D, Raftery D, Satr?stegui J, Kanow M, Chan L, Tsang SH, Sweet IR, Hurley JB
The Journal of biological chemistry291(9)4698-710(2016 Feb)

Pyruvate kinase and aspartate-glutamate carrier distributions reveal key metabolic links between neurons and glia in retina.
Lindsay KJ, Du J, Sloat SR, Contreras L, Linton JD, Turner SJ, Sadilek M, Satr?stegui J, Hurley JB
Proceedings of the National Academy of Sciences of the United States of America111(43)15579-84(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Muller glia compensate for their unique metabolic adaptations by using lactate and aspartate from neurons as surrogates for their missing PK and AGC1.
Title: Pyruvate kinase and aspartate-glutamate carrier distributions reveal key metabolic links between neurons and glia in retina.

Aralar-knockout postnatal mice show hyperactivity, anxiety-like behavior, and hyperreactivity with a decrease of dopamine (DA) in terminal-rich regions.
Title: AGC1-malate aspartate shuttle activity is critical for dopamine handling in the nigrostriatal pathway.

Aralar protein & mRNA expressions were the same in adult neurons & astrocytes, whole brain,& well-differentiated astrocyte cultures, but not until late development, like the late-maturing brain ability to form & degrade glutamate.
Title: Aralar mRNA and protein levels in neurons and astrocytes freshly isolated from young and adult mouse brain and in maturing cultured astrocytes.

Postnatal Electrophysiological Development Is Arrested in Aralar-Knockout Mice.
Title: Altered postnatal development of cortico-hippocampal neuronal electric activity in mice deficient for the mitochondrial aspartate-glutamate transporter.

the primary defect of pyramidal neurons in cerebral cortex is possibly associated with a progressive failure in glutamatergic neurotransmission and may be among the main causes of the pathology of aralar/AGC1 deficiency.
Title: Deficiency of the mitochondrial transporter of aspartate/glutamate aralar/AGC1 causes hypomyelination and neuronal defects unrelated to myelin deficits in mouse brain.

The following Slc25a12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc25a12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu31036	XM_006500420.1 Latest version!	Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu22409	NM_172436.4 Latest version!	Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$398.30 $569.00
OMu31036	XM_030252254.1 Latest version!	Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu71772	XM_011239853.3 Latest version!	Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu22409	NM_172436.3	Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$398.30 $569.00
OMu71772	XM_011239853.2	Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc25a12 ( XM_006500420.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500483.1 Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu31036
Clone ID Related Accession (Same CDS sequence)	XM_006500420.1 , XM_030252254.1
Accession Version	XM_006500420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	calcium-binding mitochondrial carrier protein Aralar1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTCGG GTCTGGATTT CAGTGACGTC ATGGTCACCA TCCGGTCCCA CATGCTCACT 
CCTTTTGTGG AGGAGAACCT GGTCTCAGCT GCCGGAGGGG GTACCTCACA CCAAGTTAGC 
TTCTCCTACT TCAACGCATT TAACTCCTTA CTCAACAACA TGGAGCTTGT TCGAAAGATC 
TATAGCACTT TAGCAGGCAC GAGGAAAGAT ATTGAAGTCA CCAAGGAGGA ATTTGCCCAG 
AGCGCCATAC GCTATGGACA GGTCACTCCA CTAGAAATTG ATATCTTGTA CCAGCTTGCA 
GACCTATATA ATGCCTCAGG GCGCTTGACT CTGGCAGACA TCGAGAGAAT AGCTCCGCTG 
GCCGAGGGGG CCTTACCTTA CAACCTGGCA GAGCTTCAGA GGCAGCAATC TCCTGGCTTG 
GGCAGACCTA TCTGGCTCCA GATTGCCGAG TCTGCATACA GGTTCACTCT GGGCTCAGTT 
GCTGGAGCTG TGGGGGCCAC TGCGGTGTAC CCCATAGACC TGGTGAAGAC CCGGATGCAA 
AACCAGCGTG GTACGGGCTC TGTGGTCGGG GAGCTGATGT ATAAAAACAG CTTCGACTGT 
TTTAAGAAAG TGTTGCGCTA CGAGGGTTTC TTTGGACTGT ACCGAGGTCT GATCCCACAG 
CTGATAGGGG TTGCACCAGA AAAAGCCATT AAGCTAACAG TTAATGATTT TGTCCGGGAC 
AAATTTACCA AAAGAGATGG CTCTATCCCA CTGCCGGCGG AAATCCTTGC TGGAGGTTGC 
GCTGGAGGCT CGCAGGTCAT CTTTACCAAC CCTCTGGAGA TAGTGAAGAT CCGCCTGCAG 
GTAGCCGGAG AGATCACCAC AGGACCGAGA GTCAGTGCTC TGAATGTGCT TCAGGACCTG 
GGACTTTTCG GGCTGTACAA GGGTGCCAAA GCCTGTTTCC TCCGAGACAT TCCCTTCTCC 
GCAATCTATT TTCCCGTGTA CGCTCACTGC AAACTCCTCC TGGCTGATGA GAACGGACGC 
GTGGGAGGCA TCAATCTCCT GACAGCGGGA GCCTTGGCAG GCGTACCTGC TGCTTCTCTG 
GTGACCCCTG CTGATGTCAT CAAGACAAGA CTGCAAGTGG CCGCCAGGGC CGGCCAGACA 
ACATACAGCG GGGTCGTCGA CTGTTTCCGA AAGATTCTCC GGGAAGAAGG GCCCTCGGCA 
TTTTGGAAAG GGACTGCAGC TCGGGTGTTT CGATCCTCAC CTCAGTTTGG TGTCACTCTG 
GTTACCTACG AGCTTCTGCA GCGGTGGTTT TACATAGATT TTGGAGGCCT CAAACCTTCT 
GGCTCAGAAC CAACGCCAAA GTCCCGCATC GCAGACCTCC CTCCTGCCAA CCCCGATCAC 
ATCGGTGGCT ACAGACTTGC CACGGCCACT TTCGCTGGCA TTGAAAACAA GTTTGGCCTT 
TACCTCCCCA AGTTTAAGTC TCCTAGCGTA GCTGTGGCTC AGCCCAAGGC GGCAGCAGCA 
GCCCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500483.1
CDS	316..1824
Translation	MISGLDFSDVMVTIRSHMLTPFVEENLVSAAGGGTSHQVSFSYFNAFNSLLNNMELVRKIYSTLAGTRKDIEVTKEEFAQSAIRYGQVTPLEIDILYQLADLYNASGRLTLADIERIAPLAEGALPYNLAELQRQQSPGLGRPIWLQIAESAYRFTLGSVAGAVGATAVYPIDLVKTRMQNQRGTGSVVGELMYKNSFDCFKKVLRYEGFFGLYRGLIPQLIGVAPEKAIKLTVNDFVRDKFTKRDGSIPLPAEILAGGCAGGSQVIFTNPLEIVKIRLQVAGEITTGPRVSALNVLQDLGLFGLYKGAKACFLRDIPFSAIYFPVYAHCKLLLADENGRVGGINLLTAGALAGVPAASLVTPADVIKTRLQVAARAGQTTYSGVVDCFRKILREEGPSAFWKGTAARVFRSSPQFGVTLVTYELLQRWFYIDFGGLKPSGSEPTPKSRIADLPPANPDHIGGYRLATATFAGIENKFGLYLPKFKSPSVAVAQPKAAAAAQ

Target ORF information:

RefSeq Version	XM_006500420.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500420.1

ORF Insert Sequence:

ATGATCTCGG GTCTGGATTT CAGTGACGTC ATGGTCACCA TCCGGTCCCA CATGCTCACT 
CCTTTTGTGG AGGAGAACCT GGTCTCAGCT GCCGGAGGGG GTACCTCACA CCAAGTTAGC 
TTCTCCTACT TCAACGCATT TAACTCCTTA CTCAACAACA TGGAGCTTGT TCGAAAGATC 
TATAGCACTT TAGCAGGCAC GAGGAAAGAT ATTGAAGTCA CCAAGGAGGA ATTTGCCCAG 
AGCGCCATAC GCTATGGACA GGTCACTCCA CTAGAAATTG ATATCTTGTA CCAGCTTGCA 
GACCTATATA ATGCCTCAGG GCGCTTGACT CTGGCAGACA TCGAGAGAAT AGCTCCGCTG 
GCCGAGGGGG CCTTACCTTA CAACCTGGCA GAGCTTCAGA GGCAGCAATC TCCTGGCTTG 
GGCAGACCTA TCTGGCTCCA GATTGCCGAG TCTGCATACA GGTTCACTCT GGGCTCAGTT 
GCTGGAGCTG TGGGGGCCAC TGCGGTGTAC CCCATAGACC TGGTGAAGAC CCGGATGCAA 
AACCAGCGTG GTACGGGCTC TGTGGTCGGG GAGCTGATGT ATAAAAACAG CTTCGACTGT 
TTTAAGAAAG TGTTGCGCTA CGAGGGTTTC TTTGGACTGT ACCGAGGTCT GATCCCACAG 
CTGATAGGGG TTGCACCAGA AAAAGCCATT AAGCTAACAG TTAATGATTT TGTCCGGGAC 
AAATTTACCA AAAGAGATGG CTCTATCCCA CTGCCGGCGG AAATCCTTGC TGGAGGTTGC 
GCTGGAGGCT CGCAGGTCAT CTTTACCAAC CCTCTGGAGA TAGTGAAGAT CCGCCTGCAG 
GTAGCCGGAG AGATCACCAC AGGACCGAGA GTCAGTGCTC TGAATGTGCT TCAGGACCTG 
GGACTTTTCG GGCTGTACAA GGGTGCCAAA GCCTGTTTCC TCCGAGACAT TCCCTTCTCC 
GCAATCTATT TTCCCGTGTA CGCTCACTGC AAACTCCTCC TGGCTGATGA GAACGGACGC 
GTGGGAGGCA TCAATCTCCT GACAGCGGGA GCCTTGGCAG GCGTACCTGC TGCTTCTCTG 
GTGACCCCTG CTGATGTCAT CAAGACAAGA CTGCAAGTGG CCGCCAGGGC CGGCCAGACA 
ACATACAGCG GGGTCGTCGA CTGTTTCCGA AAGATTCTCC GGGAAGAAGG GCCCTCGGCA 
TTTTGGAAAG GGACTGCAGC TCGGGTGTTT CGATCCTCAC CTCAGTTTGG TGTCACTCTG 
GTTACCTACG AGCTTCTGCA GCGGTGGTTT TACATAGATT TTGGAGGCCT CAAACCTTCT 
GGCTCAGAAC CAACGCCAAA GTCCCGCATC GCAGACCTCC CTCCTGCCAA CCCCGATCAC 
ATCGGTGGCT ACAGACTTGC CACGGCCACT TTCGCTGGCA TTGAAAACAA GTTTGGCCTT 
TACCTCCCCA AGTTTAAGTC TCCTAGCGTA GCTGTGGCTC AGCCCAAGGC GGCAGCAGCA 
GCCCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc25a12 ( XM_011239853.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238155.1 Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu71772
Clone ID Related Accession (Same CDS sequence)	XM_011239853.2 , XM_011239853.3
Accession Version	XM_011239853.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	calcium-binding mitochondrial carrier protein Aralar1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCATAG TGGCTTTCCA ACTGTTTGAC AAGAGCGGAA ATGGAGAGGT GACATTTGAA 
AATGTCAAGG AGATTTTTGG GCAGACTATT ATCCATCACC ACATCCCTTT TAACTGGGAC 
TGCGAGTTCA TCCGGCTGCA CTTTGGGCAC AACCGCAAGA AGCACCTGAA CTATGTGGAA 
TTCACTCAGT TCCTCCAGGA ATTGCAATTG GAACACGCAA GGCAAGCCTT TGCACTGAAA 
GACAAGAGCA AAAGCGGCAT GATCTCGGGT CTGGATTTCA GTGACGTCAT GGTCACCATC 
CGGTCCCACA TGCTCACTCC TTTTGTGGAG GAGAACCTGG TCTCAGCTGC CGGAGGGGGT 
ACCTCACACC AAGTTAGCTT CTCCTACTTC AACGCATTTA ACTCCTTACT CAACAACATG 
GAGCTTGTTC GAAAGATCTA TAGCACTTTA GCAGGCACGA GGAAAGATAT TGAAGTCACC 
AAGGAGGAAT TTGCCCAGAG CGCCATACGC TATGGACAGG TCACTCCACT AGAAATTGAT 
ATCTTGTACC AGCTTGCAGA CCTATATAAT GCCTCAGGGC GCTTGACTCT GGCAGACATC 
GAGAGAATAG CTCCGCTGGC CGAGGGGGCC TTACCTTACA ACCTGGCAGA GCTTCAGAGG 
CAGCAATCTC CTGGCTTGGG CAGACCTATC TGGCTCCAGA TTGCCGAGTC TGCATACAGG 
TTCACTCTGG GCTCAGTTGC TGGAGCTGTG GGGGCCACTG CGGTGTACCC CATAGACCTG 
GTGAAGACCC GGATGCAAAA CCAGCGTGGT ACGGGCTCTG TGGTCGGGGA GCTGATGTAT 
AAAAACAGCT TCGACTGTTT TAAGAAAGTG TTGCGCTACG AGGGTTTCTT TGGACTGTAC 
CGAGGTCTGA TCCCACAGCT GATAGGGGTT GCACCAGAAA AAGCCATTAA GCTAACAGTT 
AATGATTTTG TCCGGGACAA ATTTACCAAA AGAGATGGCT CTATCCCACT GCCGGCGGAA 
ATCCTTGCTG GAGGTTGCGC TGGAGGCTCG CAGGTCATCT TTACCAACCC TCTGGAGATA 
GTGAAGATCC GCCTGCAGGT AGCCGGAGAG ATCACCACAG GACCGAGAGT CAGTGCTCTG 
AATGTGCTTC AGGACCTGGG ACTTTTCGGG CTGTACAAGG GTGCCAAAGC CTGTTTCCTC 
CGAGACATTC CCTTCTCCGC AATCTATTTT CCCGTGTACG CTCACTGCAA ACTCCTCCTG 
GCTGATGAGA ACGGACGCGT GGGAGGCATC AATCTCCTGA CAGCGGGAGC CTTGGCAGGC 
GTACCTGCTG CTTCTCTGGT GACCCCTGCT GATGTCATCA AGACAAGACT GCAAGTGGCC 
GCCAGGGCCG GCCAGACAAC ATACAGCGGG GTCGTCGACT GTTTCCGAAA GATTCTCCGG 
GAAGAAGGGC CCTCGGCATT TTGGAAAGGG ACTGCAGCTC GGGTGTTTCG ATCCTCACCT 
CAGTTTGGTG TCACTCTGGT TACCTACGAG CTTCTGCAGC GGTGGTTTTA CATAGATTTT 
GGAGGCCTCA AACCTTCTGG CTCAGAACCA ACGCCAAAGT CCCGCATCGC AGACCTCCCT 
CCTGCCAACC CCGATCACAT CGGTGGCTAC AGACTTGCCA CGGCCACTTT CGCTGGCATT 
GAAAACAAGT TTGGCCTTTA CCTCCCCAAG TTTAAGTCTC CTAGCGTAGC TGTGGCTCAG 
CCCAAGGCGG CAGCAGCAGC CCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238155.1
CDS	5443..7209
Translation	MFIVAFQLFDKSGNGEVTFENVKEIFGQTIIHHHIPFNWDCEFIRLHFGHNRKKHLNYVEFTQFLQELQLEHARQAFALKDKSKSGMISGLDFSDVMVTIRSHMLTPFVEENLVSAAGGGTSHQVSFSYFNAFNSLLNNMELVRKIYSTLAGTRKDIEVTKEEFAQSAIRYGQVTPLEIDILYQLADLYNASGRLTLADIERIAPLAEGALPYNLAELQRQQSPGLGRPIWLQIAESAYRFTLGSVAGAVGATAVYPIDLVKTRMQNQRGTGSVVGELMYKNSFDCFKKVLRYEGFFGLYRGLIPQLIGVAPEKAIKLTVNDFVRDKFTKRDGSIPLPAEILAGGCAGGSQVIFTNPLEIVKIRLQVAGEITTGPRVSALNVLQDLGLFGLYKGAKACFLRDIPFSAIYFPVYAHCKLLLADENGRVGGINLLTAGALAGVPAASLVTPADVIKTRLQVAARAGQTTYSGVVDCFRKILREEGPSAFWKGTAARVFRSSPQFGVTLVTYELLQRWFYIDFGGLKPSGSEPTPKSRIADLPPANPDHIGGYRLATATFAGIENKFGLYLPKFKSPSVAVAQPKAAAAAQ

Target ORF information:

RefSeq Version	XM_011239853.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239853.2

ORF Insert Sequence:

ATGTTCATAG TGGCTTTCCA ACTGTTTGAC AAGAGCGGAA ATGGAGAGGT GACATTTGAA 
AATGTCAAGG AGATTTTTGG GCAGACTATT ATCCATCACC ACATCCCTTT TAACTGGGAC 
TGCGAGTTCA TCCGGCTGCA CTTTGGGCAC AACCGCAAGA AGCACCTGAA CTATGTGGAA 
TTCACTCAGT TCCTCCAGGA ATTGCAATTG GAACACGCAA GGCAAGCCTT TGCACTGAAA 
GACAAGAGCA AAAGCGGCAT GATCTCGGGT CTGGATTTCA GTGACGTCAT GGTCACCATC 
CGGTCCCACA TGCTCACTCC TTTTGTGGAG GAGAACCTGG TCTCAGCTGC CGGAGGGGGT 
ACCTCACACC AAGTTAGCTT CTCCTACTTC AACGCATTTA ACTCCTTACT CAACAACATG 
GAGCTTGTTC GAAAGATCTA TAGCACTTTA GCAGGCACGA GGAAAGATAT TGAAGTCACC 
AAGGAGGAAT TTGCCCAGAG CGCCATACGC TATGGACAGG TCACTCCACT AGAAATTGAT 
ATCTTGTACC AGCTTGCAGA CCTATATAAT GCCTCAGGGC GCTTGACTCT GGCAGACATC 
GAGAGAATAG CTCCGCTGGC CGAGGGGGCC TTACCTTACA ACCTGGCAGA GCTTCAGAGG 
CAGCAATCTC CTGGCTTGGG CAGACCTATC TGGCTCCAGA TTGCCGAGTC TGCATACAGG 
TTCACTCTGG GCTCAGTTGC TGGAGCTGTG GGGGCCACTG CGGTGTACCC CATAGACCTG 
GTGAAGACCC GGATGCAAAA CCAGCGTGGT ACGGGCTCTG TGGTCGGGGA GCTGATGTAT 
AAAAACAGCT TCGACTGTTT TAAGAAAGTG TTGCGCTACG AGGGTTTCTT TGGACTGTAC 
CGAGGTCTGA TCCCACAGCT GATAGGGGTT GCACCAGAAA AAGCCATTAA GCTAACAGTT 
AATGATTTTG TCCGGGACAA ATTTACCAAA AGAGATGGCT CTATCCCACT GCCGGCGGAA 
ATCCTTGCTG GAGGTTGCGC TGGAGGCTCG CAGGTCATCT TTACCAACCC TCTGGAGATA 
GTGAAGATCC GCCTGCAGGT AGCCGGAGAG ATCACCACAG GACCGAGAGT CAGTGCTCTG 
AATGTGCTTC AGGACCTGGG ACTTTTCGGG CTGTACAAGG GTGCCAAAGC CTGTTTCCTC 
CGAGACATTC CCTTCTCCGC AATCTATTTT CCCGTGTACG CTCACTGCAA ACTCCTCCTG 
GCTGATGAGA ACGGACGCGT GGGAGGCATC AATCTCCTGA CAGCGGGAGC CTTGGCAGGC 
GTACCTGCTG CTTCTCTGGT GACCCCTGCT GATGTCATCA AGACAAGACT GCAAGTGGCC 
GCCAGGGCCG GCCAGACAAC ATACAGCGGG GTCGTCGACT GTTTCCGAAA GATTCTCCGG 
GAAGAAGGGC CCTCGGCATT TTGGAAAGGG ACTGCAGCTC GGGTGTTTCG ATCCTCACCT 
CAGTTTGGTG TCACTCTGGT TACCTACGAG CTTCTGCAGC GGTGGTTTTA CATAGATTTT 
GGAGGCCTCA AACCTTCTGG CTCAGAACCA ACGCCAAAGT CCCGCATCGC AGACCTCCCT 
CCTGCCAACC CCGATCACAT CGGTGGCTAC AGACTTGCCA CGGCCACTTT CGCTGGCATT 
GAAAACAAGT TTGGCCTTTA CCTCCCCAAG TTTAAGTCTC CTAGCGTAGC TGTGGCTCAG 
CCCAAGGCGG CAGCAGCAGC CCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc25a12 ( NM_172436.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766024.1 Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), mRNA.

Price & Availability↑

CloneID	OMu22409
Clone ID Related Accession (Same CDS sequence)	NM_172436.4 , NM_172436.3
Accession Version	NM_172436.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	calcium-binding mitochondrial carrier protein Aralar1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX284624.4, AK083156.1 and BP765755.1. On Apr 26, 2018 this sequence version replaced NM_172436.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK144958.1, AK147948.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTCA AGGTGCATAC AACCAAACGA GGTGATCCTC ATGAGCTGAG AAACATATTC 
CTCCAGTATG CCAGCACTGA GGTGGACGGG GAGCATTACA TGACTCCAGA AGACTTCGTT 
CAACGCTATC TTGGCCTGTA CAACGATCCA AACAGCAACC CAAAGATTGT GCAGCTCTTG 
GCAGGAGTAG CTGATCAAAC CAAGGATGGG TTGATCTCCT ATCAAGAGTT TTTGGCATTT 
GAATCTGTTC TATGTGCTCC AGATTCCATG TTCATAGTGG CTTTCCAACT GTTTGACAAG 
AGCGGAAATG GAGAGGTGAC ATTTGAAAAT GTCAAGGAGA TTTTTGGGCA GACTATTATC 
CATCACCACA TCCCTTTTAA CTGGGACTGC GAGTTCATCC GGCTGCACTT TGGGCACAAC 
CGCAAGAAGC ACCTGAACTA TGTGGAATTC ACTCAGTTCC TCCAGGAATT GCAATTGGAA 
CACGCAAGGC AAGCCTTTGC ACTGAAAGAC AAGAGCAAAA GCGGCATGAT CTCGGGTCTG 
GATTTCAGTG ACGTCATGGT CACCATCCGG TCCCACATGC TCACTCCTTT TGTGGAGGAG 
AACCTGGTCT CAGCTGCCGG AGGGGGTACC TCACACCAAG TTAGCTTCTC CTACTTCAAC 
GCATTTAACT CCTTACTCAA CAACATGGAG CTTGTTCGAA AGATCTATAG CACTTTAGCA 
GGCACGAGGA AAGATATTGA AGTCACCAAG GAGGAATTTG CCCAGAGCGC CATACGCTAT 
GGACAGGTCA CTCCACTAGA AATTGATATC TTGTACCAGC TTGCAGACCT ATATAATGCC 
TCAGGGCGCT TGACTCTGGC AGACATCGAG AGAATAGCTC CGCTGGCCGA GGGGGCCTTA 
CCTTACAACC TGGCAGAGCT TCAGAGGCAG CAATCTCCTG GCTTGGGCAG ACCTATCTGG 
CTCCAGATTG CCGAGTCTGC ATACAGGTTC ACTCTGGGCT CAGTTGCTGG AGCTGTGGGG 
GCCACTGCGG TGTACCCCAT AGACCTGGTG AAGACCCGGA TGCAAAACCA GCGTGGTACG 
GGCTCTGTGG TCGGGGAGCT GATGTATAAA AACAGCTTCG ACTGTTTTAA GAAAGTGTTG 
CGCTACGAGG GTTTCTTTGG ACTGTACCGA GGTCTGATCC CACAGCTGAT AGGGGTTGCA 
CCAGAAAAAG CCATTAAGCT AACAGTTAAT GATTTTGTCC GGGACAAATT TACCAAAAGA 
GATGGCTCTA TCCCACTGCC GGCGGAAATC CTTGCTGGAG GTTGCGCTGG AGGCTCGCAG 
GTCATCTTTA CCAACCCTCT GGAGATAGTG AAGATCCGCC TGCAGGTAGC CGGAGAGATC 
ACCACAGGAC CGAGAGTCAG TGCTCTGAAT GTGCTTCAGG ACCTGGGACT TTTCGGGCTG 
TACAAGGGTG CCAAAGCCTG TTTCCTCCGA GACATTCCCT TCTCCGCAAT CTATTTTCCC 
GTGTACGCTC ACTGCAAACT CCTCCTGGCT GATGAGAACG GACGCGTGGG AGGCATCAAT 
CTCCTGACAG CGGGAGCCTT GGCAGGCGTA CCTGCTGCTT CTCTGGTGAC CCCTGCTGAT 
GTCATCAAGA CAAGACTGCA AGTGGCCGCC AGGGCCGGCC AGACAACATA CAGCGGGGTC 
GTCGACTGTT TCCGAAAGAT TCTCCGGGAA GAAGGGCCCT CGGCATTTTG GAAAGGGACT 
GCAGCTCGGG TGTTTCGATC CTCACCTCAG TTTGGTGTCA CTCTGGTTAC CTACGAGCTT 
CTGCAGCGGT GGTTTTACAT AGATTTTGGA GGCCTCAAAC CTTCTGGCTC AGAACCAACG 
CCAAAGTCCC GCATCGCAGA CCTCCCTCCT GCCAACCCCG ATCACATCGG TGGCTACAGA 
CTTGCCACGG CCACTTTCGC TGGCATTGAA AACAAGTTTG GCCTTTACCT CCCCAAGTTT 
AAGTCTCCTA GCGTAGCTGT GGCTCAGCCC AAGGCGGCAG CAGCAGCCCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766024.1
CDS	232..2265
Translation	MAVKVHTTKRGDPHELRNIFLQYASTEVDGEHYMTPEDFVQRYLGLYNDPNSNPKIVQLLAGVADQTKDGLISYQEFLAFESVLCAPDSMFIVAFQLFDKSGNGEVTFENVKEIFGQTIIHHHIPFNWDCEFIRLHFGHNRKKHLNYVEFTQFLQELQLEHARQAFALKDKSKSGMISGLDFSDVMVTIRSHMLTPFVEENLVSAAGGGTSHQVSFSYFNAFNSLLNNMELVRKIYSTLAGTRKDIEVTKEEFAQSAIRYGQVTPLEIDILYQLADLYNASGRLTLADIERIAPLAEGALPYNLAELQRQQSPGLGRPIWLQIAESAYRFTLGSVAGAVGATAVYPIDLVKTRMQNQRGTGSVVGELMYKNSFDCFKKVLRYEGFFGLYRGLIPQLIGVAPEKAIKLTVNDFVRDKFTKRDGSIPLPAEILAGGCAGGSQVIFTNPLEIVKIRLQVAGEITTGPRVSALNVLQDLGLFGLYKGAKACFLRDIPFSAIYFPVYAHCKLLLADENGRVGGINLLTAGALAGVPAASLVTPADVIKTRLQVAARAGQTTYSGVVDCFRKILREEGPSAFWKGTAARVFRSSPQFGVTLVTYELLQRWFYIDFGGLKPSGSEPTPKSRIADLPPANPDHIGGYRLATATFAGIENKFGLYLPKFKSPSVAVAQPKAAAAAQ

Target ORF information:

RefSeq Version	NM_172436.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172436.4

ORF Insert Sequence:

ATGGCGGTCA AGGTGCATAC AACCAAACGA GGTGATCCTC ATGAGCTGAG AAACATATTC 
CTCCAGTATG CCAGCACTGA GGTGGACGGG GAGCATTACA TGACTCCAGA AGACTTCGTT 
CAACGCTATC TTGGCCTGTA CAACGATCCA AACAGCAACC CAAAGATTGT GCAGCTCTTG 
GCAGGAGTAG CTGATCAAAC CAAGGATGGG TTGATCTCCT ATCAAGAGTT TTTGGCATTT 
GAATCTGTTC TATGTGCTCC AGATTCCATG TTCATAGTGG CTTTCCAACT GTTTGACAAG 
AGCGGAAATG GAGAGGTGAC ATTTGAAAAT GTCAAGGAGA TTTTTGGGCA GACTATTATC 
CATCACCACA TCCCTTTTAA CTGGGACTGC GAGTTCATCC GGCTGCACTT TGGGCACAAC 
CGCAAGAAGC ACCTGAACTA TGTGGAATTC ACTCAGTTCC TCCAGGAATT GCAATTGGAA 
CACGCAAGGC AAGCCTTTGC ACTGAAAGAC AAGAGCAAAA GCGGCATGAT CTCGGGTCTG 
GATTTCAGTG ACGTCATGGT CACCATCCGG TCCCACATGC TCACTCCTTT TGTGGAGGAG 
AACCTGGTCT CAGCTGCCGG AGGGGGTACC TCACACCAAG TTAGCTTCTC CTACTTCAAC 
GCATTTAACT CCTTACTCAA CAACATGGAG CTTGTTCGAA AGATCTATAG CACTTTAGCA 
GGCACGAGGA AAGATATTGA AGTCACCAAG GAGGAATTTG CCCAGAGCGC CATACGCTAT 
GGACAGGTCA CTCCACTAGA AATTGATATC TTGTACCAGC TTGCAGACCT ATATAATGCC 
TCAGGGCGCT TGACTCTGGC AGACATCGAG AGAATAGCTC CGCTGGCCGA GGGGGCCTTA 
CCTTACAACC TGGCAGAGCT TCAGAGGCAG CAATCTCCTG GCTTGGGCAG ACCTATCTGG 
CTCCAGATTG CCGAGTCTGC ATACAGGTTC ACTCTGGGCT CAGTTGCTGG AGCTGTGGGG 
GCCACTGCGG TGTACCCCAT AGACCTGGTG AAGACCCGGA TGCAAAACCA GCGTGGTACG 
GGCTCTGTGG TCGGGGAGCT GATGTATAAA AACAGCTTCG ACTGTTTTAA GAAAGTGTTG 
CGCTACGAGG GTTTCTTTGG ACTGTACCGA GGTCTGATCC CACAGCTGAT AGGGGTTGCA 
CCAGAAAAAG CCATTAAGCT AACAGTTAAT GATTTTGTCC GGGACAAATT TACCAAAAGA 
GATGGCTCTA TCCCACTGCC GGCGGAAATC CTTGCTGGAG GTTGCGCTGG AGGCTCGCAG 
GTCATCTTTA CCAACCCTCT GGAGATAGTG AAGATCCGCC TGCAGGTAGC CGGAGAGATC 
ACCACAGGAC CGAGAGTCAG TGCTCTGAAT GTGCTTCAGG ACCTGGGACT TTTCGGGCTG 
TACAAGGGTG CCAAAGCCTG TTTCCTCCGA GACATTCCCT TCTCCGCAAT CTATTTTCCC 
GTGTACGCTC ACTGCAAACT CCTCCTGGCT GATGAGAACG GACGCGTGGG AGGCATCAAT 
CTCCTGACAG CGGGAGCCTT GGCAGGCGTA CCTGCTGCTT CTCTGGTGAC CCCTGCTGAT 
GTCATCAAGA CAAGACTGCA AGTGGCCGCC AGGGCCGGCC AGACAACATA CAGCGGGGTC 
GTCGACTGTT TCCGAAAGAT TCTCCGGGAA GAAGGGCCCT CGGCATTTTG GAAAGGGACT 
GCAGCTCGGG TGTTTCGATC CTCACCTCAG TTTGGTGTCA CTCTGGTTAC CTACGAGCTT 
CTGCAGCGGT GGTTTTACAT AGATTTTGGA GGCCTCAAAC CTTCTGGCTC AGAACCAACG 
CCAAAGTCCC GCATCGCAGA CCTCCCTCCT GCCAACCCCG ATCACATCGG TGGCTACAGA 
CTTGCCACGG CCACTTTCGC TGGCATTGAA AACAAGTTTG GCCTTTACCT CCCCAAGTTT 
AAGTCTCCTA GCGTAGCTGT GGCTCAGCCC AAGGCGGCAG CAGCAGCCCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc25a12 ( NM_172436.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766024.1 Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), mRNA.

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CloneID	OMu22409
Clone ID Related Accession (Same CDS sequence)	NM_172436.4 , NM_172436.3
Accession Version	NM_172436.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	calcium-binding mitochondrial carrier protein Aralar1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK083156.1. On Apr 5, 2007 this sequence version replaced NM_172436.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK083156.1, AK144958.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCGGTCA AGGTGCATAC AACCAAACGA GGTGATCCTC ATGAGCTGAG AAACATATTC 
CTCCAGTATG CCAGCACTGA GGTGGACGGG GAGCATTACA TGACTCCAGA AGACTTCGTT 
CAACGCTATC TTGGCCTGTA CAACGATCCA AACAGCAACC CAAAGATTGT GCAGCTCTTG 
GCAGGAGTAG CTGATCAAAC CAAGGATGGG TTGATCTCCT ATCAAGAGTT TTTGGCATTT 
GAATCTGTTC TATGTGCTCC AGATTCCATG TTCATAGTGG CTTTCCAACT GTTTGACAAG 
AGCGGAAATG GAGAGGTGAC ATTTGAAAAT GTCAAGGAGA TTTTTGGGCA GACTATTATC 
CATCACCACA TCCCTTTTAA CTGGGACTGC GAGTTCATCC GGCTGCACTT TGGGCACAAC 
CGCAAGAAGC ACCTGAACTA TGTGGAATTC ACTCAGTTCC TCCAGGAATT GCAATTGGAA 
CACGCAAGGC AAGCCTTTGC ACTGAAAGAC AAGAGCAAAA GCGGCATGAT CTCGGGTCTG 
GATTTCAGTG ACGTCATGGT CACCATCCGG TCCCACATGC TCACTCCTTT TGTGGAGGAG 
AACCTGGTCT CAGCTGCCGG AGGGGGTACC TCACACCAAG TTAGCTTCTC CTACTTCAAC 
GCATTTAACT CCTTACTCAA CAACATGGAG CTTGTTCGAA AGATCTATAG CACTTTAGCA 
GGCACGAGGA AAGATATTGA AGTCACCAAG GAGGAATTTG CCCAGAGCGC CATACGCTAT 
GGACAGGTCA CTCCACTAGA AATTGATATC TTGTACCAGC TTGCAGACCT ATATAATGCC 
TCAGGGCGCT TGACTCTGGC AGACATCGAG AGAATAGCTC CGCTGGCCGA GGGGGCCTTA 
CCTTACAACC TGGCAGAGCT TCAGAGGCAG CAATCTCCTG GCTTGGGCAG ACCTATCTGG 
CTCCAGATTG CCGAGTCTGC ATACAGGTTC ACTCTGGGCT CAGTTGCTGG AGCTGTGGGG 
GCCACTGCGG TGTACCCCAT AGACCTGGTG AAGACCCGGA TGCAAAACCA GCGTGGTACG 
GGCTCTGTGG TCGGGGAGCT GATGTATAAA AACAGCTTCG ACTGTTTTAA GAAAGTGTTG 
CGCTACGAGG GTTTCTTTGG ACTGTACCGA GGTCTGATCC CACAGCTGAT AGGGGTTGCA 
CCAGAAAAAG CCATTAAGCT AACAGTTAAT GATTTTGTCC GGGACAAATT TACCAAAAGA 
GATGGCTCTA TCCCACTGCC GGCGGAAATC CTTGCTGGAG GTTGCGCTGG AGGCTCGCAG 
GTCATCTTTA CCAACCCTCT GGAGATAGTG AAGATCCGCC TGCAGGTAGC CGGAGAGATC 
ACCACAGGAC CGAGAGTCAG TGCTCTGAAT GTGCTTCAGG ACCTGGGACT TTTCGGGCTG 
TACAAGGGTG CCAAAGCCTG TTTCCTCCGA GACATTCCCT TCTCCGCAAT CTATTTTCCC 
GTGTACGCTC ACTGCAAACT CCTCCTGGCT GATGAGAACG GACGCGTGGG AGGCATCAAT 
CTCCTGACAG CGGGAGCCTT GGCAGGCGTA CCTGCTGCTT CTCTGGTGAC CCCTGCTGAT 
GTCATCAAGA CAAGACTGCA AGTGGCCGCC AGGGCCGGCC AGACAACATA CAGCGGGGTC 
GTCGACTGTT TCCGAAAGAT TCTCCGGGAA GAAGGGCCCT CGGCATTTTG GAAAGGGACT 
GCAGCTCGGG TGTTTCGATC CTCACCTCAG TTTGGTGTCA CTCTGGTTAC CTACGAGCTT 
CTGCAGCGGT GGTTTTACAT AGATTTTGGA GGCCTCAAAC CTTCTGGCTC AGAACCAACG 
CCAAAGTCCC GCATCGCAGA CCTCCCTCCT GCCAACCCCG ATCACATCGG TGGCTACAGA 
CTTGCCACGG CCACTTTCGC TGGCATTGAA AACAAGTTTG GCCTTTACCT CCCCAAGTTT 
AAGTCTCCTA GCGTAGCTGT GGCTCAGCCC AAGGCGGCAG CAGCAGCCCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766024.1
CDS	31..2064
Translation	MAVKVHTTKRGDPHELRNIFLQYASTEVDGEHYMTPEDFVQRYLGLYNDPNSNPKIVQLLAGVADQTKDGLISYQEFLAFESVLCAPDSMFIVAFQLFDKSGNGEVTFENVKEIFGQTIIHHHIPFNWDCEFIRLHFGHNRKKHLNYVEFTQFLQELQLEHARQAFALKDKSKSGMISGLDFSDVMVTIRSHMLTPFVEENLVSAAGGGTSHQVSFSYFNAFNSLLNNMELVRKIYSTLAGTRKDIEVTKEEFAQSAIRYGQVTPLEIDILYQLADLYNASGRLTLADIERIAPLAEGALPYNLAELQRQQSPGLGRPIWLQIAESAYRFTLGSVAGAVGATAVYPIDLVKTRMQNQRGTGSVVGELMYKNSFDCFKKVLRYEGFFGLYRGLIPQLIGVAPEKAIKLTVNDFVRDKFTKRDGSIPLPAEILAGGCAGGSQVIFTNPLEIVKIRLQVAGEITTGPRVSALNVLQDLGLFGLYKGAKACFLRDIPFSAIYFPVYAHCKLLLADENGRVGGINLLTAGALAGVPAASLVTPADVIKTRLQVAARAGQTTYSGVVDCFRKILREEGPSAFWKGTAARVFRSSPQFGVTLVTYELLQRWFYIDFGGLKPSGSEPTPKSRIADLPPANPDHIGGYRLATATFAGIENKFGLYLPKFKSPSVAVAQPKAAAAAQ

Target ORF information:

RefSeq Version	NM_172436.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172436.3

ORF Insert Sequence:

ATGGCGGTCA AGGTGCATAC AACCAAACGA GGTGATCCTC ATGAGCTGAG AAACATATTC 
CTCCAGTATG CCAGCACTGA GGTGGACGGG GAGCATTACA TGACTCCAGA AGACTTCGTT 
CAACGCTATC TTGGCCTGTA CAACGATCCA AACAGCAACC CAAAGATTGT GCAGCTCTTG 
GCAGGAGTAG CTGATCAAAC CAAGGATGGG TTGATCTCCT ATCAAGAGTT TTTGGCATTT 
GAATCTGTTC TATGTGCTCC AGATTCCATG TTCATAGTGG CTTTCCAACT GTTTGACAAG 
AGCGGAAATG GAGAGGTGAC ATTTGAAAAT GTCAAGGAGA TTTTTGGGCA GACTATTATC 
CATCACCACA TCCCTTTTAA CTGGGACTGC GAGTTCATCC GGCTGCACTT TGGGCACAAC 
CGCAAGAAGC ACCTGAACTA TGTGGAATTC ACTCAGTTCC TCCAGGAATT GCAATTGGAA 
CACGCAAGGC AAGCCTTTGC ACTGAAAGAC AAGAGCAAAA GCGGCATGAT CTCGGGTCTG 
GATTTCAGTG ACGTCATGGT CACCATCCGG TCCCACATGC TCACTCCTTT TGTGGAGGAG 
AACCTGGTCT CAGCTGCCGG AGGGGGTACC TCACACCAAG TTAGCTTCTC CTACTTCAAC 
GCATTTAACT CCTTACTCAA CAACATGGAG CTTGTTCGAA AGATCTATAG CACTTTAGCA 
GGCACGAGGA AAGATATTGA AGTCACCAAG GAGGAATTTG CCCAGAGCGC CATACGCTAT 
GGACAGGTCA CTCCACTAGA AATTGATATC TTGTACCAGC TTGCAGACCT ATATAATGCC 
TCAGGGCGCT TGACTCTGGC AGACATCGAG AGAATAGCTC CGCTGGCCGA GGGGGCCTTA 
CCTTACAACC TGGCAGAGCT TCAGAGGCAG CAATCTCCTG GCTTGGGCAG ACCTATCTGG 
CTCCAGATTG CCGAGTCTGC ATACAGGTTC ACTCTGGGCT CAGTTGCTGG AGCTGTGGGG 
GCCACTGCGG TGTACCCCAT AGACCTGGTG AAGACCCGGA TGCAAAACCA GCGTGGTACG 
GGCTCTGTGG TCGGGGAGCT GATGTATAAA AACAGCTTCG ACTGTTTTAA GAAAGTGTTG 
CGCTACGAGG GTTTCTTTGG ACTGTACCGA GGTCTGATCC CACAGCTGAT AGGGGTTGCA 
CCAGAAAAAG CCATTAAGCT AACAGTTAAT GATTTTGTCC GGGACAAATT TACCAAAAGA 
GATGGCTCTA TCCCACTGCC GGCGGAAATC CTTGCTGGAG GTTGCGCTGG AGGCTCGCAG 
GTCATCTTTA CCAACCCTCT GGAGATAGTG AAGATCCGCC TGCAGGTAGC CGGAGAGATC 
ACCACAGGAC CGAGAGTCAG TGCTCTGAAT GTGCTTCAGG ACCTGGGACT TTTCGGGCTG 
TACAAGGGTG CCAAAGCCTG TTTCCTCCGA GACATTCCCT TCTCCGCAAT CTATTTTCCC 
GTGTACGCTC ACTGCAAACT CCTCCTGGCT GATGAGAACG GACGCGTGGG AGGCATCAAT 
CTCCTGACAG CGGGAGCCTT GGCAGGCGTA CCTGCTGCTT CTCTGGTGAC CCCTGCTGAT 
GTCATCAAGA CAAGACTGCA AGTGGCCGCC AGGGCCGGCC AGACAACATA CAGCGGGGTC 
GTCGACTGTT TCCGAAAGAT TCTCCGGGAA GAAGGGCCCT CGGCATTTTG GAAAGGGACT 
GCAGCTCGGG TGTTTCGATC CTCACCTCAG TTTGGTGTCA CTCTGGTTAC CTACGAGCTT 
CTGCAGCGGT GGTTTTACAT AGATTTTGGA GGCCTCAAAC CTTCTGGCTC AGAACCAACG 
CCAAAGTCCC GCATCGCAGA CCTCCCTCCT GCCAACCCCG ATCACATCGG TGGCTACAGA 
CTTGCCACGG CCACTTTCGC TGGCATTGAA AACAAGTTTG GCCTTTACCT CCCCAAGTTT 
AAGTCTCCTA GCGTAGCTGT GGCTCAGCCC AAGGCGGCAG CAGCAGCCCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc25a12 ( XM_030252254.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108114.1 Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), transcript variant X2, mRNA.

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CloneID	OMu31036
Clone ID Related Accession (Same CDS sequence)	XM_006500420.1 , XM_030252254.1
Accession Version	XM_030252254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calcium-binding mitochondrial carrier protein Aralar1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTCGG GTCTGGATTT CAGTGACGTC ATGGTCACCA TCCGGTCCCA CATGCTCACT 
CCTTTTGTGG AGGAGAACCT GGTCTCAGCT GCCGGAGGGG GTACCTCACA CCAAGTTAGC 
TTCTCCTACT TCAACGCATT TAACTCCTTA CTCAACAACA TGGAGCTTGT TCGAAAGATC 
TATAGCACTT TAGCAGGCAC GAGGAAAGAT ATTGAAGTCA CCAAGGAGGA ATTTGCCCAG 
AGCGCCATAC GCTATGGACA GGTCACTCCA CTAGAAATTG ATATCTTGTA CCAGCTTGCA 
GACCTATATA ATGCCTCAGG GCGCTTGACT CTGGCAGACA TCGAGAGAAT AGCTCCGCTG 
GCCGAGGGGG CCTTACCTTA CAACCTGGCA GAGCTTCAGA GGCAGCAATC TCCTGGCTTG 
GGCAGACCTA TCTGGCTCCA GATTGCCGAG TCTGCATACA GGTTCACTCT GGGCTCAGTT 
GCTGGAGCTG TGGGGGCCAC TGCGGTGTAC CCCATAGACC TGGTGAAGAC CCGGATGCAA 
AACCAGCGTG GTACGGGCTC TGTGGTCGGG GAGCTGATGT ATAAAAACAG CTTCGACTGT 
TTTAAGAAAG TGTTGCGCTA CGAGGGTTTC TTTGGACTGT ACCGAGGTCT GATCCCACAG 
CTGATAGGGG TTGCACCAGA AAAAGCCATT AAGCTAACAG TTAATGATTT TGTCCGGGAC 
AAATTTACCA AAAGAGATGG CTCTATCCCA CTGCCGGCGG AAATCCTTGC TGGAGGTTGC 
GCTGGAGGCT CGCAGGTCAT CTTTACCAAC CCTCTGGAGA TAGTGAAGAT CCGCCTGCAG 
GTAGCCGGAG AGATCACCAC AGGACCGAGA GTCAGTGCTC TGAATGTGCT TCAGGACCTG 
GGACTTTTCG GGCTGTACAA GGGTGCCAAA GCCTGTTTCC TCCGAGACAT TCCCTTCTCC 
GCAATCTATT TTCCCGTGTA CGCTCACTGC AAACTCCTCC TGGCTGATGA GAACGGACGC 
GTGGGAGGCA TCAATCTCCT GACAGCGGGA GCCTTGGCAG GCGTACCTGC TGCTTCTCTG 
GTGACCCCTG CTGATGTCAT CAAGACAAGA CTGCAAGTGG CCGCCAGGGC CGGCCAGACA 
ACATACAGCG GGGTCGTCGA CTGTTTCCGA AAGATTCTCC GGGAAGAAGG GCCCTCGGCA 
TTTTGGAAAG GGACTGCAGC TCGGGTGTTT CGATCCTCAC CTCAGTTTGG TGTCACTCTG 
GTTACCTACG AGCTTCTGCA GCGGTGGTTT TACATAGATT TTGGAGGCCT CAAACCTTCT 
GGCTCAGAAC CAACGCCAAA GTCCCGCATC GCAGACCTCC CTCCTGCCAA CCCCGATCAC 
ATCGGTGGCT ACAGACTTGC CACGGCCACT TTCGCTGGCA TTGAAAACAA GTTTGGCCTT 
TACCTCCCCA AGTTTAAGTC TCCTAGCGTA GCTGTGGCTC AGCCCAAGGC GGCAGCAGCA 
GCCCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108114.1
CDS	887..2395
Translation	MISGLDFSDVMVTIRSHMLTPFVEENLVSAAGGGTSHQVSFSYFNAFNSLLNNMELVRKIYSTLAGTRKDIEVTKEEFAQSAIRYGQVTPLEIDILYQLADLYNASGRLTLADIERIAPLAEGALPYNLAELQRQQSPGLGRPIWLQIAESAYRFTLGSVAGAVGATAVYPIDLVKTRMQNQRGTGSVVGELMYKNSFDCFKKVLRYEGFFGLYRGLIPQLIGVAPEKAIKLTVNDFVRDKFTKRDGSIPLPAEILAGGCAGGSQVIFTNPLEIVKIRLQVAGEITTGPRVSALNVLQDLGLFGLYKGAKACFLRDIPFSAIYFPVYAHCKLLLADENGRVGGINLLTAGALAGVPAASLVTPADVIKTRLQVAARAGQTTYSGVVDCFRKILREEGPSAFWKGTAARVFRSSPQFGVTLVTYELLQRWFYIDFGGLKPSGSEPTPKSRIADLPPANPDHIGGYRLATATFAGIENKFGLYLPKFKSPSVAVAQPKAAAAAQ

Target ORF information:

RefSeq Version	XM_030252254.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252254.1

ORF Insert Sequence:

ATGATCTCGG GTCTGGATTT CAGTGACGTC ATGGTCACCA TCCGGTCCCA CATGCTCACT 
CCTTTTGTGG AGGAGAACCT GGTCTCAGCT GCCGGAGGGG GTACCTCACA CCAAGTTAGC 
TTCTCCTACT TCAACGCATT TAACTCCTTA CTCAACAACA TGGAGCTTGT TCGAAAGATC 
TATAGCACTT TAGCAGGCAC GAGGAAAGAT ATTGAAGTCA CCAAGGAGGA ATTTGCCCAG 
AGCGCCATAC GCTATGGACA GGTCACTCCA CTAGAAATTG ATATCTTGTA CCAGCTTGCA 
GACCTATATA ATGCCTCAGG GCGCTTGACT CTGGCAGACA TCGAGAGAAT AGCTCCGCTG 
GCCGAGGGGG CCTTACCTTA CAACCTGGCA GAGCTTCAGA GGCAGCAATC TCCTGGCTTG 
GGCAGACCTA TCTGGCTCCA GATTGCCGAG TCTGCATACA GGTTCACTCT GGGCTCAGTT 
GCTGGAGCTG TGGGGGCCAC TGCGGTGTAC CCCATAGACC TGGTGAAGAC CCGGATGCAA 
AACCAGCGTG GTACGGGCTC TGTGGTCGGG GAGCTGATGT ATAAAAACAG CTTCGACTGT 
TTTAAGAAAG TGTTGCGCTA CGAGGGTTTC TTTGGACTGT ACCGAGGTCT GATCCCACAG 
CTGATAGGGG TTGCACCAGA AAAAGCCATT AAGCTAACAG TTAATGATTT TGTCCGGGAC 
AAATTTACCA AAAGAGATGG CTCTATCCCA CTGCCGGCGG AAATCCTTGC TGGAGGTTGC 
GCTGGAGGCT CGCAGGTCAT CTTTACCAAC CCTCTGGAGA TAGTGAAGAT CCGCCTGCAG 
GTAGCCGGAG AGATCACCAC AGGACCGAGA GTCAGTGCTC TGAATGTGCT TCAGGACCTG 
GGACTTTTCG GGCTGTACAA GGGTGCCAAA GCCTGTTTCC TCCGAGACAT TCCCTTCTCC 
GCAATCTATT TTCCCGTGTA CGCTCACTGC AAACTCCTCC TGGCTGATGA GAACGGACGC 
GTGGGAGGCA TCAATCTCCT GACAGCGGGA GCCTTGGCAG GCGTACCTGC TGCTTCTCTG 
GTGACCCCTG CTGATGTCAT CAAGACAAGA CTGCAAGTGG CCGCCAGGGC CGGCCAGACA 
ACATACAGCG GGGTCGTCGA CTGTTTCCGA AAGATTCTCC GGGAAGAAGG GCCCTCGGCA 
TTTTGGAAAG GGACTGCAGC TCGGGTGTTT CGATCCTCAC CTCAGTTTGG TGTCACTCTG 
GTTACCTACG AGCTTCTGCA GCGGTGGTTT TACATAGATT TTGGAGGCCT CAAACCTTCT 
GGCTCAGAAC CAACGCCAAA GTCCCGCATC GCAGACCTCC CTCCTGCCAA CCCCGATCAC 
ATCGGTGGCT ACAGACTTGC CACGGCCACT TTCGCTGGCA TTGAAAACAA GTTTGGCCTT 
TACCTCCCCA AGTTTAAGTC TCCTAGCGTA GCTGTGGCTC AGCCCAAGGC GGCAGCAGCA 
GCCCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc25a12 ( XM_011239853.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238155.1 Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), transcript variant X1, mRNA.

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CloneID	OMu71772
Clone ID Related Accession (Same CDS sequence)	XM_011239853.2 , XM_011239853.3
Accession Version	XM_011239853.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calcium-binding mitochondrial carrier protein Aralar1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011239853.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCATAG TGGCTTTCCA ACTGTTTGAC AAGAGCGGAA ATGGAGAGGT GACATTTGAA 
AATGTCAAGG AGATTTTTGG GCAGACTATT ATCCATCACC ACATCCCTTT TAACTGGGAC 
TGCGAGTTCA TCCGGCTGCA CTTTGGGCAC AACCGCAAGA AGCACCTGAA CTATGTGGAA 
TTCACTCAGT TCCTCCAGGA ATTGCAATTG GAACACGCAA GGCAAGCCTT TGCACTGAAA 
GACAAGAGCA AAAGCGGCAT GATCTCGGGT CTGGATTTCA GTGACGTCAT GGTCACCATC 
CGGTCCCACA TGCTCACTCC TTTTGTGGAG GAGAACCTGG TCTCAGCTGC CGGAGGGGGT 
ACCTCACACC AAGTTAGCTT CTCCTACTTC AACGCATTTA ACTCCTTACT CAACAACATG 
GAGCTTGTTC GAAAGATCTA TAGCACTTTA GCAGGCACGA GGAAAGATAT TGAAGTCACC 
AAGGAGGAAT TTGCCCAGAG CGCCATACGC TATGGACAGG TCACTCCACT AGAAATTGAT 
ATCTTGTACC AGCTTGCAGA CCTATATAAT GCCTCAGGGC GCTTGACTCT GGCAGACATC 
GAGAGAATAG CTCCGCTGGC CGAGGGGGCC TTACCTTACA ACCTGGCAGA GCTTCAGAGG 
CAGCAATCTC CTGGCTTGGG CAGACCTATC TGGCTCCAGA TTGCCGAGTC TGCATACAGG 
TTCACTCTGG GCTCAGTTGC TGGAGCTGTG GGGGCCACTG CGGTGTACCC CATAGACCTG 
GTGAAGACCC GGATGCAAAA CCAGCGTGGT ACGGGCTCTG TGGTCGGGGA GCTGATGTAT 
AAAAACAGCT TCGACTGTTT TAAGAAAGTG TTGCGCTACG AGGGTTTCTT TGGACTGTAC 
CGAGGTCTGA TCCCACAGCT GATAGGGGTT GCACCAGAAA AAGCCATTAA GCTAACAGTT 
AATGATTTTG TCCGGGACAA ATTTACCAAA AGAGATGGCT CTATCCCACT GCCGGCGGAA 
ATCCTTGCTG GAGGTTGCGC TGGAGGCTCG CAGGTCATCT TTACCAACCC TCTGGAGATA 
GTGAAGATCC GCCTGCAGGT AGCCGGAGAG ATCACCACAG GACCGAGAGT CAGTGCTCTG 
AATGTGCTTC AGGACCTGGG ACTTTTCGGG CTGTACAAGG GTGCCAAAGC CTGTTTCCTC 
CGAGACATTC CCTTCTCCGC AATCTATTTT CCCGTGTACG CTCACTGCAA ACTCCTCCTG 
GCTGATGAGA ACGGACGCGT GGGAGGCATC AATCTCCTGA CAGCGGGAGC CTTGGCAGGC 
GTACCTGCTG CTTCTCTGGT GACCCCTGCT GATGTCATCA AGACAAGACT GCAAGTGGCC 
GCCAGGGCCG GCCAGACAAC ATACAGCGGG GTCGTCGACT GTTTCCGAAA GATTCTCCGG 
GAAGAAGGGC CCTCGGCATT TTGGAAAGGG ACTGCAGCTC GGGTGTTTCG ATCCTCACCT 
CAGTTTGGTG TCACTCTGGT TACCTACGAG CTTCTGCAGC GGTGGTTTTA CATAGATTTT 
GGAGGCCTCA AACCTTCTGG CTCAGAACCA ACGCCAAAGT CCCGCATCGC AGACCTCCCT 
CCTGCCAACC CCGATCACAT CGGTGGCTAC AGACTTGCCA CGGCCACTTT CGCTGGCATT 
GAAAACAAGT TTGGCCTTTA CCTCCCCAAG TTTAAGTCTC CTAGCGTAGC TGTGGCTCAG 
CCCAAGGCGG CAGCAGCAGC CCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238155.1
CDS	5780..7546
Translation	MFIVAFQLFDKSGNGEVTFENVKEIFGQTIIHHHIPFNWDCEFIRLHFGHNRKKHLNYVEFTQFLQELQLEHARQAFALKDKSKSGMISGLDFSDVMVTIRSHMLTPFVEENLVSAAGGGTSHQVSFSYFNAFNSLLNNMELVRKIYSTLAGTRKDIEVTKEEFAQSAIRYGQVTPLEIDILYQLADLYNASGRLTLADIERIAPLAEGALPYNLAELQRQQSPGLGRPIWLQIAESAYRFTLGSVAGAVGATAVYPIDLVKTRMQNQRGTGSVVGELMYKNSFDCFKKVLRYEGFFGLYRGLIPQLIGVAPEKAIKLTVNDFVRDKFTKRDGSIPLPAEILAGGCAGGSQVIFTNPLEIVKIRLQVAGEITTGPRVSALNVLQDLGLFGLYKGAKACFLRDIPFSAIYFPVYAHCKLLLADENGRVGGINLLTAGALAGVPAASLVTPADVIKTRLQVAARAGQTTYSGVVDCFRKILREEGPSAFWKGTAARVFRSSPQFGVTLVTYELLQRWFYIDFGGLKPSGSEPTPKSRIADLPPANPDHIGGYRLATATFAGIENKFGLYLPKFKSPSVAVAQPKAAAAAQ

Target ORF information:

RefSeq Version	XM_011239853.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (Slc25a12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239853.3

ORF Insert Sequence:

ATGTTCATAG TGGCTTTCCA ACTGTTTGAC AAGAGCGGAA ATGGAGAGGT GACATTTGAA 
AATGTCAAGG AGATTTTTGG GCAGACTATT ATCCATCACC ACATCCCTTT TAACTGGGAC 
TGCGAGTTCA TCCGGCTGCA CTTTGGGCAC AACCGCAAGA AGCACCTGAA CTATGTGGAA 
TTCACTCAGT TCCTCCAGGA ATTGCAATTG GAACACGCAA GGCAAGCCTT TGCACTGAAA 
GACAAGAGCA AAAGCGGCAT GATCTCGGGT CTGGATTTCA GTGACGTCAT GGTCACCATC 
CGGTCCCACA TGCTCACTCC TTTTGTGGAG GAGAACCTGG TCTCAGCTGC CGGAGGGGGT 
ACCTCACACC AAGTTAGCTT CTCCTACTTC AACGCATTTA ACTCCTTACT CAACAACATG 
GAGCTTGTTC GAAAGATCTA TAGCACTTTA GCAGGCACGA GGAAAGATAT TGAAGTCACC 
AAGGAGGAAT TTGCCCAGAG CGCCATACGC TATGGACAGG TCACTCCACT AGAAATTGAT 
ATCTTGTACC AGCTTGCAGA CCTATATAAT GCCTCAGGGC GCTTGACTCT GGCAGACATC 
GAGAGAATAG CTCCGCTGGC CGAGGGGGCC TTACCTTACA ACCTGGCAGA GCTTCAGAGG 
CAGCAATCTC CTGGCTTGGG CAGACCTATC TGGCTCCAGA TTGCCGAGTC TGCATACAGG 
TTCACTCTGG GCTCAGTTGC TGGAGCTGTG GGGGCCACTG CGGTGTACCC CATAGACCTG 
GTGAAGACCC GGATGCAAAA CCAGCGTGGT ACGGGCTCTG TGGTCGGGGA GCTGATGTAT 
AAAAACAGCT TCGACTGTTT TAAGAAAGTG TTGCGCTACG AGGGTTTCTT TGGACTGTAC 
CGAGGTCTGA TCCCACAGCT GATAGGGGTT GCACCAGAAA AAGCCATTAA GCTAACAGTT 
AATGATTTTG TCCGGGACAA ATTTACCAAA AGAGATGGCT CTATCCCACT GCCGGCGGAA 
ATCCTTGCTG GAGGTTGCGC TGGAGGCTCG CAGGTCATCT TTACCAACCC TCTGGAGATA 
GTGAAGATCC GCCTGCAGGT AGCCGGAGAG ATCACCACAG GACCGAGAGT CAGTGCTCTG 
AATGTGCTTC AGGACCTGGG ACTTTTCGGG CTGTACAAGG GTGCCAAAGC CTGTTTCCTC 
CGAGACATTC CCTTCTCCGC AATCTATTTT CCCGTGTACG CTCACTGCAA ACTCCTCCTG 
GCTGATGAGA ACGGACGCGT GGGAGGCATC AATCTCCTGA CAGCGGGAGC CTTGGCAGGC 
GTACCTGCTG CTTCTCTGGT GACCCCTGCT GATGTCATCA AGACAAGACT GCAAGTGGCC 
GCCAGGGCCG GCCAGACAAC ATACAGCGGG GTCGTCGACT GTTTCCGAAA GATTCTCCGG 
GAAGAAGGGC CCTCGGCATT TTGGAAAGGG ACTGCAGCTC GGGTGTTTCG ATCCTCACCT 
CAGTTTGGTG TCACTCTGGT TACCTACGAG CTTCTGCAGC GGTGGTTTTA CATAGATTTT 
GGAGGCCTCA AACCTTCTGG CTCAGAACCA ACGCCAAAGT CCCGCATCGC AGACCTCCCT 
CCTGCCAACC CCGATCACAT CGGTGGCTAC AGACTTGCCA CGGCCACTTT CGCTGGCATT 
GAAAACAAGT TTGGCCTTTA CCTCCCCAAG TTTAAGTCTC CTAGCGTAGC TGTGGCTCAG 
CCCAAGGCGG CAGCAGCAGC CCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ARALAR/AGC1 deficiency, a neurodevelopmental disorder with severe impairment of neuronal mitochondrial respiration, does not produce a primary increase in brain lactate.
Journal of neurochemistry142(1)132-139(2017 Jul)
Juaristi I,Garc?a-Mart?n ML,Rodrigues TB,Satr?stegui J,Llorente-Folch I,Pardo B

L-Lactate-Mediated Neuroprotection against Glutamate-Induced Excitotoxicity Requires ARALAR/AGC1.
The Journal of neuroscience : the official journal of the Society for Neuroscience36(16)4443-56(2016 Apr)
Llorente-Folch I,Rueda CB,P?rez-Li?bana I,Satr?stegui J,Pardo B

Phototransduction Influences Metabolic Flux and Nucleotide Metabolism in Mouse Retina.
The Journal of biological chemistry291(9)4698-710(2016 Feb)
Du J,Rountree A,Cleghorn WM,Contreras L,Lindsay KJ,Sadilek M,Gu H,Djukovic D,Raftery D,Satr?stegui J,Kanow M,Chan L,Tsang SH,Sweet IR,Hurley JB

Pyruvate kinase and aspartate-glutamate carrier distributions reveal key metabolic links between neurons and glia in retina.
Proceedings of the National Academy of Sciences of the United States of America111(43)15579-84(2014 Oct)
Lindsay KJ,Du J,Sloat SR,Contreras L,Linton JD,Turner SJ,Sadilek M,Satr?stegui J,Hurley JB

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Slc25a12 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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