Adgra2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adgra2
Entrez Gene ID	78560
Full Name	adhesion G protein-coupled receptor A2
Synonyms	8430414O08Rik,9530074E10Rik,Gpr124,Tem5,mKIAA1531
General protein information	Preferred Names adhesion G protein-coupled receptor A2 Names adhesion G protein-coupled receptor A2 G protein coupled receptor Gpr124 G-protein coupled receptor 124 tumor endothelial marker 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 A2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006509223.4	XP_006509286.3	adhesion G protein-coupled receptor A2 isoform X2
XM_006509224.3	XP_006509287.3	adhesion G protein-coupled receptor A2 isoform X3
NM_054044.2	NP_473385.2	adhesion G protein-coupled receptor A2 precursor
XM_006509222.3	XP_006509285.3	adhesion G protein-coupled receptor A2 isoform X1
XM_006509223.3	XP_006509286.3	adhesion G protein-coupled receptor A2 isoform X2
XM_006509222.4	XP_006509285.3	adhesion G protein-coupled receptor A2 isoform X1
XM_006509224.4	XP_006509287.3	adhesion G protein-coupled receptor A2 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GPR124	XP_528111.2
Canis lupus familiaris (dog)	GPR124	XP_539968.4
Gallus gallus (chicken)	GPR124	XP_003642599.2
Danio rerio (zebrafish)	gpr124	XP_689189.6
Rattus norvegicus (Norway rat)	LOC100363275	XP_003751665.2
Mus musculus (house mouse)	Gpr124	NP_473385.2
Xenopus tropicalis (tropical clawed frog)	gpr124	XP_002933000.1
Homo sapiens (human)	GPR124	NP_116166.9
	GPR124	XP_001089205.2

Gene Ontology
Bibliography

Related articles in PubMed

Reck and Gpr124 Are Essential Receptor Cofactors for Wnt7a/Wnt7b-Specific Signaling in Mammalian CNS Angiogenesis and Blood-Brain Barrier Regulation.
Cho C, Smallwood PM, Nathans J
Neuron95(5)1056-1073.e5(2017 Aug)

Gpr124 is essential for blood-brain barrier integrity in central nervous system disease.
Chang J, Mancuso MR, Maier C, Liang X, Yuki K, Yang L, Kwong JW, Wang J, Rao V, Vallon M, Kosinski C, Zhang JJ, Mah AT, Xu L, Li L, Gholamin S, Reyes TF, Li R, Kuhnert F, Han X, Yuan J, Chiou SH, Brettman AD, Daly L, Corney DC, Cheshier SH, Shortliffe LD, Wu X, Snyder M, Chan P, Giffard RG, Chang HY, Andreasson K, Kuo CJ
Nature medicine23(4)450-460(2017 Apr)

Early molecular events during retinoic acid induced differentiation of neuromesodermal progenitors.
Cunningham TJ, Colas A, Duester G
Biology open5(12)1821-1833(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

experiments indicate that Reck and Gpr124 are part of the cell surface protein complex that transduces Wnt7a- and Wnt7b-specific signals in mammalian CNS Epithelial Cells to promote angiogenesis and regulate the BBB.
Title: Reck and Gpr124 Are Essential Receptor Cofactors for Wnt7a/Wnt7b-Specific Signaling in Mammalian CNS Angiogenesis and Blood-Brain Barrier Regulation.

Gpr124 is an endothelial GPCR specifically required for endothelial Wnt signaling and BBB integrity under pathological conditions in adult mice.
Title: Gpr124 is essential for blood-brain barrier integrity in central nervous system disease.

Vital role for GPR124 is potentiation of WNT7-induced canonical beta-catenin signaling with important implications for understanding and manipulating CNS-specific angiogenesis.
Title: GPR124 functions as a WNT7-specific coactivator of canonical ?-catenin signaling.

Gpr124 functions as coactivator of Wnt7a- and Wnt7b-stimulated canonical Wnt signaling via Frizzled receptor and Lrp coreceptor and that Gpr124-stimulated signaling functions in concert with Norrin/Frizzled4 signaling to control CNS vascular development
Title: Gpr124 controls CNS angiogenesis and blood-brain barrier integrity by promoting ligand-specific canonical wnt signaling.

GPR124, an orphan G protein-coupled receptor, is required for CNS-specific vascularization and establishment of the blood-brain barrier.
Title: GPR124, an orphan G protein-coupled receptor, is required for CNS-specific vascularization and establishment of the blood-brain barrier.

The following Adgra2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adgra2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu82608	XM_006509224.4 Latest version!	Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu82606	XM_006509222.4 Latest version!	Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu82607	XM_006509223.4 Latest version!	Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu13324	NM_054044.2 Latest version!	Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OMu82607	XM_006509223.3	Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu82606	XM_006509222.3	Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu82608	XM_006509224.3	Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adgra2 ( XM_006509224.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509287.3 Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu82608
Clone ID Related Accession (Same CDS sequence)	XM_006509224.3 , XM_006509224.4
Accession Version	XM_006509224.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor A2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006509224.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCC GCCCCGCCCT CGGGCGCCCG GGACTCCCGC TGAGCACTCC TCCCGCACGC 
CCGGGTCCCT CCGGCCGGCG AGCCGTCCGG CCCCTGCGCT GTAGGTCCCC GCGGGGCGAT 
GGGTTGATGG GCGCGGGGGG ACGCAGGATG CCGGTGCCTC CCGCGCGTTT GCTGCTGCTG 
CCGCTGCTGC CTTGTCTTCT GCTCCTGGCT CCTGGAACTC GGGGTGCGCC TGGCTGCCCG 
GTCCCTATCC GCGGTTGCAA GTGCTCTGGG GAGCGGCCCA AGGGACTAAG TGGCGGCGCC 
CACAACCCGG CTCGAAGGAG GGTGGTGTGC GGCGGTGGGG ATCTCCCCGA ACCTCCAGAT 
CCCGGCCTTC TGCCAAACGG CACCATCACC TTGCTCTTGA GCAACAACAA GATTACTGGG 
CTCCGCAATG GATCCTTCTT GGGACTGTCC CTGTTGGAGA AGTTGGACCT GAGGAGCAAT 
GTCATCAGCA CTGTGCAGCC TGGAGCCTTC CTAGGTCTGG GAGAGCTAAA ACGCTTAGAT 
CTCTCCAACA ATCGGATTGG CTGTCTCACC TCTGAGACAT TTCAAGGGCT CCCTAGACTT 
CTCAGACTAA ACATATCTGG AAACATCTAC TCTAGTCTGC AACCTGGGGT CTTTGATGAG 
CTGCCAGCCC TTAAGATTGT GGACTTTGGT ACTGAGTTTC TGACCTGTGA CTGCCGCCTG 
CGCTGGCTGC TGCCCTGGGC CCGGAATCAC TCCCTGCAGC TGTCTGAGCG CACACTCTGT 
GCCTACCCCA GTGCCCTGCA CGCCCATGCC CTGAGCAGCC TCCAGGAGTC CCAGCTTCGC 
TGTGAAGGGG CCCTGGAACT GCACACCCAC TACCTCATCC CATCCCTTCG CCAAGTGGTG 
TTCCAGGGTG ACCGCCTGCC CTTCCAGTGC TCAGCCAGCT ACTTGGGCAA CGATACCCGG 
ATCCACTGGT ACCACAATGG GGCTCCTATG GAGAGCGATG AGCAGGCCGG CATCGTCCTT 
GCTGAAAACC TTATCCATGA TTGCACCTTC ATCACCAGTG AGCTGACCCT GTCTCACATT 
GGTGTGTGGG CCTCTGGTGA ATGGGAGTGC TCCGTGTCCA CAGTCCAAGG CAACACCAGC 
AAGAAGGTGG AGATAGTAGT GCTGGAGACC TCTGCCTCCT ACTGCCCTGC AGAGCGGGTG 
ACCAACAACC GTGGGGACTT CAGGTGGCCC CGAACCTTGG CTGGCATCAC GGCTTACCAG 
TCCTGTTTAC AGTACCCCTT CACCTCTGTG CCCTTGAGTG GGGGAGCCCC GGGTACCCGA 
GCCTCACGCA GGTGTGACCG AGCTGGCCGC TGGGAGCCAG GGGACTACTC GCACTGTCTG 
TACACCAATG ACATCACTCG GGTGCTCTAC ACCTTTGTGC TGATGCCCAT CAACGCCTCC 
AATGCATTGA CGTTGGCCCA CCAGCTGCGA GTGTATACCG CAGAGGCCGC CAGCTTCTCA 
GACATGATGG ACGTGGTCTA TGTGGCTCAG ATGATCCAGA AGTTTTTGGG TTACGTTGAC 
CAGATCAAGG AGCTGGTGGA GGTGATGGTG GACATGGCCA GCAACCTGAT GCTGGTGGAT 
GAGCACCTTC TGTGGCTGGC CCAGAGAGAA GACAAAGCCT GCAGTGGCAT TGTGGGTGCC 
CTGGAGCGAA TCGGAGGAGC TGCTCTTAGC CCCCATGCCC AGCACATCTC TGTGAATTCA 
AGAAATGTGG CACTGGAGGC CTACCTCATC AAGCCTCACA GCTACGTGGG TCTGACTTGC 
ACGGCTTTCC AGAGGAGGGA GGTAGGAGTG TCGGGTGCAC AGCCAAGCAG CGTCGGCCAG 
GATGCCCCAG TGGAGCCCGA ACCCCTAGCT GATCAGCAGC TTAGGTTCCG CTGCACCACT 
GGGAGGCCCA ACATTTCTCT GTCATCCTTC CACATCAAGA ATAGCGTGGC CCTGGCCTCC 
ATCCAGCTGC CCCCCAGCCT GTTCTCAACC CTTCCGGCTG CCCTGGCTCC CCCAGTCCCT 
CCAGATTGCA CCCTGCAACT GCTGGTCTTC AGAAATGGCC GTCTTTTCCG CAGCCACGGC 
AACAACACTT CCCGTCCTGG AGCAGCTGGG CCTGGCAAGA GGCGTGGTGT GGCCACCCCA 
GTCATATTTG CAGGAACCAG TGGCTGTGGT GTGGGAAACT TGACGGAGCC CGTGGCTGTG 
TCACTGAGGC ACTGGGCTGA AGGAGCTGAC CCCATGGCAG CTTGGTGGAA CCAGGACGGG 
CCTGGAGGCT GGAGTTCTGA AGGCTGCAGG CTCCGCTACA GCCAGCCCAA CGTCAGCTCC 
CTGTACTGCC AGCACTTGGG CAATGTGGCC GTGCTTATGG AGCTGAATGC ATTCCCGCGG 
GAGGCAGGAG GCTCTGGGGC TGGGTTGCAT CCCGTCGTGT ACCCCTGCAC GGCTTTGCTG 
CTACTCTGTC TCTTCTCCAC CATCATCACG TACATCCTCA ACCACAGCTC CATCCACGTG 
TCCCGGAAGG GTTGGCACAT GCTGCTGAAC CTTTGTTTCC ATATGGCCAT GACCTCTGCT 
GTCTTCGTGG GGGGCGTCAC GCTCACCAAC TACCAAATGG TTTGTCAAGC GGGTATAGCC 
CAGTCAGCAA CTCAGGTCCC AAGCCTCGGT GCCTTGACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509287.3
CDS	301..2985
Translation	MAPRPALGRPGLPLSTPPARPGPSGRRAVRPLRCRSPRGDGLMGAGGRRMPVPPARLLLLPLLPCLLLLAPGTRGAPGCPVPIRGCKCSGERPKGLSGGAHNPARRRVVCGGGDLPEPPDPGLLPNGTITLLLSNNKITGLRNGSFLGLSLLEKLDLRSNVISTVQPGAFLGLGELKRLDLSNNRIGCLTSETFQGLPRLLRLNISGNIYSSLQPGVFDELPALKIVDFGTEFLTCDCRLRWLLPWARNHSLQLSERTLCAYPSALHAHALSSLQESQLRCEGALELHTHYLIPSLRQVVFQGDRLPFQCSASYLGNDTRIHWYHNGAPMESDEQAGIVLAENLIHDCTFITSELTLSHIGVWASGEWECSVSTVQGNTSKKVEIVVLETSASYCPAERVTNNRGDFRWPRTLAGITAYQSCLQYPFTSVPLSGGAPGTRASRRCDRAGRWEPGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDMASNLMLVDEHLLWLAQREDKACSGIVGALERIGGAALSPHAQHISVNSRNVALEAYLIKPHSYVGLTCTAFQRREVGVSGAQPSSVGQDAPVEPEPLADQQLRFRCTTGRPNISLSSFHIKNSVALASIQLPPSLFSTLPAALAPPVPPDCTLQLLVFRNGRLFRSHGNNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSLRHWAEGADPMAAWWNQDGPGGWSSEGCRLRYSQPNVSSLYCQHLGNVAVLMELNAFPREAGGSGAGLHPVVYPCTALLLLCLFSTIITYILNHSSIHVSRKGWHMLLNLCFHMAMTSAVFVGGVTLTNYQMVCQAGIAQSATQVPSLGALTL

Target ORF information:

RefSeq Version	XM_006509224.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509224.4

ORF Insert Sequence:

ATGGCCCCCC GCCCCGCCCT CGGGCGCCCG GGACTCCCGC TGAGCACTCC TCCCGCACGC 
CCGGGTCCCT CCGGCCGGCG AGCCGTCCGG CCCCTGCGCT GTAGGTCCCC GCGGGGCGAT 
GGGTTGATGG GCGCGGGGGG ACGCAGGATG CCGGTGCCTC CCGCGCGTTT GCTGCTGCTG 
CCGCTGCTGC CTTGTCTTCT GCTCCTGGCT CCTGGAACTC GGGGTGCGCC TGGCTGCCCG 
GTCCCTATCC GCGGTTGCAA GTGCTCTGGG GAGCGGCCCA AGGGACTAAG TGGCGGCGCC 
CACAACCCGG CTCGAAGGAG GGTGGTGTGC GGCGGTGGGG ATCTCCCCGA ACCTCCAGAT 
CCCGGCCTTC TGCCAAACGG CACCATCACC TTGCTCTTGA GCAACAACAA GATTACTGGG 
CTCCGCAATG GATCCTTCTT GGGACTGTCC CTGTTGGAGA AGTTGGACCT GAGGAGCAAT 
GTCATCAGCA CTGTGCAGCC TGGAGCCTTC CTAGGTCTGG GAGAGCTAAA ACGCTTAGAT 
CTCTCCAACA ATCGGATTGG CTGTCTCACC TCTGAGACAT TTCAAGGGCT CCCTAGACTT 
CTCAGACTAA ACATATCTGG AAACATCTAC TCTAGTCTGC AACCTGGGGT CTTTGATGAG 
CTGCCAGCCC TTAAGATTGT GGACTTTGGT ACTGAGTTTC TGACCTGTGA CTGCCGCCTG 
CGCTGGCTGC TGCCCTGGGC CCGGAATCAC TCCCTGCAGC TGTCTGAGCG CACACTCTGT 
GCCTACCCCA GTGCCCTGCA CGCCCATGCC CTGAGCAGCC TCCAGGAGTC CCAGCTTCGC 
TGTGAAGGGG CCCTGGAACT GCACACCCAC TACCTCATCC CATCCCTTCG CCAAGTGGTG 
TTCCAGGGTG ACCGCCTGCC CTTCCAGTGC TCAGCCAGCT ACTTGGGCAA CGATACCCGG 
ATCCACTGGT ACCACAATGG GGCTCCTATG GAGAGCGATG AGCAGGCCGG CATCGTCCTT 
GCTGAAAACC TTATCCATGA TTGCACCTTC ATCACCAGTG AGCTGACCCT GTCTCACATT 
GGTGTGTGGG CCTCTGGTGA ATGGGAGTGC TCCGTGTCCA CAGTCCAAGG CAACACCAGC 
AAGAAGGTGG AGATAGTAGT GCTGGAGACC TCTGCCTCCT ACTGCCCTGC AGAGCGGGTG 
ACCAACAACC GTGGGGACTT CAGGTGGCCC CGAACCTTGG CTGGCATCAC GGCTTACCAG 
TCCTGTTTAC AGTACCCCTT CACCTCTGTG CCCTTGAGTG GGGGAGCCCC GGGTACCCGA 
GCCTCACGCA GGTGTGACCG AGCTGGCCGC TGGGAGCCAG GGGACTACTC GCACTGTCTG 
TACACCAATG ACATCACTCG GGTGCTCTAC ACCTTTGTGC TGATGCCCAT CAACGCCTCC 
AATGCATTGA CGTTGGCCCA CCAGCTGCGA GTGTATACCG CAGAGGCCGC CAGCTTCTCA 
GACATGATGG ACGTGGTCTA TGTGGCTCAG ATGATCCAGA AGTTTTTGGG TTACGTTGAC 
CAGATCAAGG AGCTGGTGGA GGTGATGGTG GACATGGCCA GCAACCTGAT GCTGGTGGAT 
GAGCACCTTC TGTGGCTGGC CCAGAGAGAA GACAAAGCCT GCAGTGGCAT TGTGGGTGCC 
CTGGAGCGAA TCGGAGGAGC TGCTCTTAGC CCCCATGCCC AGCACATCTC TGTGAATTCA 
AGAAATGTGG CACTGGAGGC CTACCTCATC AAGCCTCACA GCTACGTGGG TCTGACTTGC 
ACGGCTTTCC AGAGGAGGGA GGTAGGAGTG TCGGGTGCAC AGCCAAGCAG CGTCGGCCAG 
GATGCCCCAG TGGAGCCCGA ACCCCTAGCT GATCAGCAGC TTAGGTTCCG CTGCACCACT 
GGGAGGCCCA ACATTTCTCT GTCATCCTTC CACATCAAGA ATAGCGTGGC CCTGGCCTCC 
ATCCAGCTGC CCCCCAGCCT GTTCTCAACC CTTCCGGCTG CCCTGGCTCC CCCAGTCCCT 
CCAGATTGCA CCCTGCAACT GCTGGTCTTC AGAAATGGCC GTCTTTTCCG CAGCCACGGC 
AACAACACTT CCCGTCCTGG AGCAGCTGGG CCTGGCAAGA GGCGTGGTGT GGCCACCCCA 
GTCATATTTG CAGGAACCAG TGGCTGTGGT GTGGGAAACT TGACGGAGCC CGTGGCTGTG 
TCACTGAGGC ACTGGGCTGA AGGAGCTGAC CCCATGGCAG CTTGGTGGAA CCAGGACGGG 
CCTGGAGGCT GGAGTTCTGA AGGCTGCAGG CTCCGCTACA GCCAGCCCAA CGTCAGCTCC 
CTGTACTGCC AGCACTTGGG CAATGTGGCC GTGCTTATGG AGCTGAATGC ATTCCCGCGG 
GAGGCAGGAG GCTCTGGGGC TGGGTTGCAT CCCGTCGTGT ACCCCTGCAC GGCTTTGCTG 
CTACTCTGTC TCTTCTCCAC CATCATCACG TACATCCTCA ACCACAGCTC CATCCACGTG 
TCCCGGAAGG GTTGGCACAT GCTGCTGAAC CTTTGTTTCC ATATGGCCAT GACCTCTGCT 
GTCTTCGTGG GGGGCGTCAC GCTCACCAAC TACCAAATGG TTTGTCAAGC GGGTATAGCC 
CAGTCAGCAA CTCAGGTCCC AAGCCTCGGT GCCTTGACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgra2 ( XM_006509222.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509285.3 Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu82606
Clone ID Related Accession (Same CDS sequence)	XM_006509222.3 , XM_006509222.4
Accession Version	XM_006509222.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor A2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006509222.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCC GCCCCGCCCT CGGGCGCCCG GGACTCCCGC TGAGCACTCC TCCCGCACGC 
CCGGGTCCCT CCGGCCGGCG AGCCGTCCGG CCCCTGCGCT GTAGGTCCCC GCGGGGCGAT 
GGGTTGATGG GCGCGGGGGG ACGCAGGATG CCGGTGCCTC CCGCGCGTTT GCTGCTGCTG 
CCGCTGCTGC CTTGTCTTCT GCTCCTGGCT CCTGGAACTC GGGGTGCGCC TGGCTGCCCG 
GTCCCTATCC GCGGTTGCAA GTGCTCTGGG GAGCGGCCCA AGGGACTAAG TGGCGGCGCC 
CACAACCCGG CTCGAAGGAG GGTGGTGTGC GGCGGTGGGG ATCTCCCCGA ACCTCCAGAT 
CCCGGCCTTC TGCCAAACGG CACCATCACC TTGCTCTTGA GCAACAACAA GATTACTGGG 
CTCCGCAATG GATCCTTCTT GGGACTGTCC CTGTTGGAGA AGTTGGACCT GAGGAGCAAT 
GTCATCAGCA CTGTGCAGCC TGGAGCCTTC CTAGGTCTGG GAGAGCTAAA ACGCTTAGAT 
CTCTCCAACA ATCGGATTGG CTGTCTCACC TCTGAGACAT TTCAAGGGCT CCCTAGACTT 
CTCAGACTAA ACATATCTGG AAACATCTAC TCTAGTCTGC AACCTGGGGT CTTTGATGAG 
CTGCCAGCCC TTAAGATTGT GGACTTTGGT ACTGAGTTTC TGACCTGTGA CTGCCGCCTG 
CGCTGGCTGC TGCCCTGGGC CCGGAATCAC TCCCTGCAGC TGTCTGAGCG CACACTCTGT 
GCCTACCCCA GTGCCCTGCA CGCCCATGCC CTGAGCAGCC TCCAGGAGTC CCAGCTTCGC 
TGTGAAGGGG CCCTGGAACT GCACACCCAC TACCTCATCC CATCCCTTCG CCAAGTGGTG 
TTCCAGGGTG ACCGCCTGCC CTTCCAGTGC TCAGCCAGCT ACTTGGGCAA CGATACCCGG 
ATCCACTGGT ACCACAATGG GGCTCCTATG GAGAGCGATG AGCAGGCCGG CATCGTCCTT 
GCTGAAAACC TTATCCATGA TTGCACCTTC ATCACCAGTG AGCTGACCCT GTCTCACATT 
GGTGTGTGGG CCTCTGGTGA ATGGGAGTGC TCCGTGTCCA CAGTCCAAGG CAACACCAGC 
AAGAAGGTGG AGATAGTAGT GCTGGAGACC TCTGCCTCCT ACTGCCCTGC AGAGCGGGTG 
ACCAACAACC GTGGGGACTT CAGGTGGCCC CGAACCTTGG CTGGCATCAC GGCTTACCAG 
TCCTGTTTAC AGTACCCCTT CACCTCTGTG CCCTTGAGTG GGGGAGCCCC GGGTACCCGA 
GCCTCACGCA GGTGTGACCG AGCTGGCCGC TGGGAGCCAG GGGACTACTC GCACTGTCTG 
TACACCAATG ACATCACTCG GGTGCTCTAC ACCTTTGTGC TGATGCCCAT CAACGCCTCC 
AATGCATTGA CGTTGGCCCA CCAGCTGCGA GTGTATACCG CAGAGGCCGC CAGCTTCTCA 
GACATGATGG ACGTGGTCTA TGTGGCTCAG ATGATCCAGA AGTTTTTGGG TTACGTTGAC 
CAGATCAAGG AGCTGGTGGA GGTGATGGTG GACATGGCCA GCAACCTGAT GCTGGTGGAT 
GAGCACCTTC TGTGGCTGGC CCAGAGAGAA GACAAAGCCT GCAGTGGCAT TGTGGGTGCC 
CTGGAGCGAA TCGGAGGAGC TGCTCTTAGC CCCCATGCCC AGCACATCTC TGTGAATTCA 
AGAAATGTGG CACTGGAGGC CTACCTCATC AAGCCTCACA GCTACGTGGG TCTGACTTGC 
ACGGCTTTCC AGAGGAGGGA GGTAGGAGTG TCGGGTGCAC AGCCAAGCAG CGTCGGCCAG 
GATGCCCCAG TGGAGCCCGA ACCCCTAGCT GATCAGCAGC TTAGGTTCCG CTGCACCACT 
GGGAGGCCCA ACATTTCTCT GTCATCCTTC CACATCAAGA ATAGCGTGGC CCTGGCCTCC 
ATCCAGCTGC CCCCCAGCCT GTTCTCAACC CTTCCGGCTG CCCTGGCTCC CCCAGTCCCT 
CCAGATTGCA CCCTGCAACT GCTGGTCTTC AGAAATGGCC GTCTTTTCCG CAGCCACGGC 
AACAACACTT CCCGTCCTGG AGCAGCTGGG CCTGGCAAGA GGCGTGGTGT GGCCACCCCA 
GTCATATTTG CAGGAACCAG TGGCTGTGGT GTGGGAAACT TGACGGAGCC CGTGGCTGTG 
TCACTGAGGC ACTGGGCTGA AGGAGCTGAC CCCATGGCAG CTTGGTGGAA CCAGGACGGG 
CCTGGAGGCT GGAGTTCTGA AGGCTGCAGG CTCCGCTACA GCCAGCCCAA CGTCAGCTCC 
CTGTACTGCC AGCACTTGGG CAATGTGGCC GTGCTTATGG AGCTGAATGC ATTCCCGCGG 
GAGGCAGGAG GCTCTGGGGC TGGGTTGCAT CCCGTCGTGT ACCCCTGCAC GGCTTTGCTG 
CTACTCTGTC TCTTCTCCAC CATCATCACG TACATCCTCA ACCACAGCTC CATCCACGTG 
TCCCGGAAGG GTTGGCACAT GCTGCTGAAC CTTTGTTTCC ATATGGCCAT GACCTCTGCT 
GTCTTCGTGG GGGGCGTCAC GCTCACCAAC TACCAAATGG TTTGTCAAGC GGTGGGCATC 
ACTCTGCACT ACTCTTCCTT GTCGTCACTG CTCTGGATGG GGGTCAAGGC TCGAGTCCTC 
CACAAGGAGC TTAGTTGGAG GGCACCCCCT CTGGAAGAAG GGGAAGCAGC TCCGCCTGGT 
CCTCGCCCCA TGCTCCGTTG TTGGCTGGTG TGGCGTCCAA GCCTTGGTGC CTTCTACATA 
CCGGTGGCGT TGATTCTGCC TATCACCTGG ATCTACTTCT TGTGTGCAGG CCTTCACTTA 
CGGAGCCATG TGGCCCAGAA TCCAAAGCAG GGTAACAGGA TCTCTCTGGA GCCAGGGGAA 
GAGCTGAGGG GTTCCACCAG GCTCAGGAGT AGTGGCGTCC TCCTGAATGA CTCTGGTTCT 
CTTTTGGCTA CAGTTAGCGC AGGAGTAGGG ACACCTGCGC CCCCAGAGGA TGGTGATGGC 
GTATATTCTC CTGGAGTCCA GCTGGGGGCG CTGATGACCA CGCATTTCCT GTACCTGGCT 
ATGTGGGCTT GTGGTGCCTT GGCGGTGTCT CAGCGCTGGC TGCCCCGAGT GGTGTGTAGC 
TGTCTGTACG GCGTGGCAGC TTCAGCTCTT GGTCTGTTTG TCTTCACTCA CCACTGTGCC 
AGACGTAGAG ATGTCCGGGC TTCCTGGCGC GCCTGCTGCC CTCCTGCTTC GCCCTCGGCC 
TCCCATGTCC CAGCCCGGGC CCTGCCGACT GCTACAGAGG ATGGATCCCC AGTGTTGGGG 
GAGGGACCAG CCTCTCTCAA GTCCTCCCCA AGTGGCAGCA GTGGCCGCGC GCCGCCACCC 
CCCTGCAAAC TCACCAATCT GCAGGTGGCC CAGAGTCAGG TGTGCGAGGC AAGCGTGGCC 
GCCCGCGGAG ATGGAGAGCC AGAGCCCACC GGCTCCCGTG GCAGCCTAGC TCCCCGGCAC 
CATAACAACC TGCATCATGG GCGCCGAGTA CACAAGAGTC GGGCCAAGGG GCACCGAGCC 
GGAGAGACTG GTGGTAAGAG CCGGCTCAAG GCGTTGCGCG CGGGCACGTC CCCAGGAGCT 
CCCGAGCTGT TGTCCAGTGA GAGCGGCAGT TTGCACAACA GCCCGTCTGA CAGCTACCCG 
GGCAGCAGCC GCAACAGTCC AGGCGACGGT CTTCCACTCG AGGGTGAGCC CATGCTTACG 
CCGTCGGAGG GCAGTGACAC AAGCGCCGCG CCAATCGCTG AGACGGGGCG CCCCGGGCAG 
CGCCGCAGCG CCAGCCGTGA CAACCTCAAG GGCAGCGGCA GCGCGCTGGA GAGGGAGAGC 
AAGCGCCGCT CCTATCCGCT CAACACTACC AGTCTTAACG GTGCCCCCAA AGGGGGCAAG 
TATGAGGATG CCAGTGTAAC TGGTGCAGAG GCCATAGCGG GAGGCTCCAT GAAGACTGGC 
CTCTGGAAAA GCGAGACCAC CGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509285.3
CDS	301..4347
Translation	MAPRPALGRPGLPLSTPPARPGPSGRRAVRPLRCRSPRGDGLMGAGGRRMPVPPARLLLLPLLPCLLLLAPGTRGAPGCPVPIRGCKCSGERPKGLSGGAHNPARRRVVCGGGDLPEPPDPGLLPNGTITLLLSNNKITGLRNGSFLGLSLLEKLDLRSNVISTVQPGAFLGLGELKRLDLSNNRIGCLTSETFQGLPRLLRLNISGNIYSSLQPGVFDELPALKIVDFGTEFLTCDCRLRWLLPWARNHSLQLSERTLCAYPSALHAHALSSLQESQLRCEGALELHTHYLIPSLRQVVFQGDRLPFQCSASYLGNDTRIHWYHNGAPMESDEQAGIVLAENLIHDCTFITSELTLSHIGVWASGEWECSVSTVQGNTSKKVEIVVLETSASYCPAERVTNNRGDFRWPRTLAGITAYQSCLQYPFTSVPLSGGAPGTRASRRCDRAGRWEPGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDMASNLMLVDEHLLWLAQREDKACSGIVGALERIGGAALSPHAQHISVNSRNVALEAYLIKPHSYVGLTCTAFQRREVGVSGAQPSSVGQDAPVEPEPLADQQLRFRCTTGRPNISLSSFHIKNSVALASIQLPPSLFSTLPAALAPPVPPDCTLQLLVFRNGRLFRSHGNNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSLRHWAEGADPMAAWWNQDGPGGWSSEGCRLRYSQPNVSSLYCQHLGNVAVLMELNAFPREAGGSGAGLHPVVYPCTALLLLCLFSTIITYILNHSSIHVSRKGWHMLLNLCFHMAMTSAVFVGGVTLTNYQMVCQAVGITLHYSSLSSLLWMGVKARVLHKELSWRAPPLEEGEAAPPGPRPMLRCWLVWRPSLGAFYIPVALILPITWIYFLCAGLHLRSHVAQNPKQGNRISLEPGEELRGSTRLRSSGVLLNDSGSLLATVSAGVGTPAPPEDGDGVYSPGVQLGALMTTHFLYLAMWACGALAVSQRWLPRVVCSCLYGVAASALGLFVFTHHCARRRDVRASWRACCPPASPSASHVPARALPTATEDGSPVLGEGPASLKSSPSGSSGRAPPPPCKLTNLQVAQSQVCEASVAARGDGEPEPTGSRGSLAPRHHNNLHHGRRVHKSRAKGHRAGETGGKSRLKALRAGTSPGAPELLSSESGSLHNSPSDSYPGSSRNSPGDGLPLEGEPMLTPSEGSDTSAAPIAETGRPGQRRSASRDNLKGSGSALERESKRRSYPLNTTSLNGAPKGGKYEDASVTGAEAIAGGSMKTGLWKSETTV

Target ORF information:

RefSeq Version	XM_006509222.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509222.4

ORF Insert Sequence:

ATGGCCCCCC GCCCCGCCCT CGGGCGCCCG GGACTCCCGC TGAGCACTCC TCCCGCACGC 
CCGGGTCCCT CCGGCCGGCG AGCCGTCCGG CCCCTGCGCT GTAGGTCCCC GCGGGGCGAT 
GGGTTGATGG GCGCGGGGGG ACGCAGGATG CCGGTGCCTC CCGCGCGTTT GCTGCTGCTG 
CCGCTGCTGC CTTGTCTTCT GCTCCTGGCT CCTGGAACTC GGGGTGCGCC TGGCTGCCCG 
GTCCCTATCC GCGGTTGCAA GTGCTCTGGG GAGCGGCCCA AGGGACTAAG TGGCGGCGCC 
CACAACCCGG CTCGAAGGAG GGTGGTGTGC GGCGGTGGGG ATCTCCCCGA ACCTCCAGAT 
CCCGGCCTTC TGCCAAACGG CACCATCACC TTGCTCTTGA GCAACAACAA GATTACTGGG 
CTCCGCAATG GATCCTTCTT GGGACTGTCC CTGTTGGAGA AGTTGGACCT GAGGAGCAAT 
GTCATCAGCA CTGTGCAGCC TGGAGCCTTC CTAGGTCTGG GAGAGCTAAA ACGCTTAGAT 
CTCTCCAACA ATCGGATTGG CTGTCTCACC TCTGAGACAT TTCAAGGGCT CCCTAGACTT 
CTCAGACTAA ACATATCTGG AAACATCTAC TCTAGTCTGC AACCTGGGGT CTTTGATGAG 
CTGCCAGCCC TTAAGATTGT GGACTTTGGT ACTGAGTTTC TGACCTGTGA CTGCCGCCTG 
CGCTGGCTGC TGCCCTGGGC CCGGAATCAC TCCCTGCAGC TGTCTGAGCG CACACTCTGT 
GCCTACCCCA GTGCCCTGCA CGCCCATGCC CTGAGCAGCC TCCAGGAGTC CCAGCTTCGC 
TGTGAAGGGG CCCTGGAACT GCACACCCAC TACCTCATCC CATCCCTTCG CCAAGTGGTG 
TTCCAGGGTG ACCGCCTGCC CTTCCAGTGC TCAGCCAGCT ACTTGGGCAA CGATACCCGG 
ATCCACTGGT ACCACAATGG GGCTCCTATG GAGAGCGATG AGCAGGCCGG CATCGTCCTT 
GCTGAAAACC TTATCCATGA TTGCACCTTC ATCACCAGTG AGCTGACCCT GTCTCACATT 
GGTGTGTGGG CCTCTGGTGA ATGGGAGTGC TCCGTGTCCA CAGTCCAAGG CAACACCAGC 
AAGAAGGTGG AGATAGTAGT GCTGGAGACC TCTGCCTCCT ACTGCCCTGC AGAGCGGGTG 
ACCAACAACC GTGGGGACTT CAGGTGGCCC CGAACCTTGG CTGGCATCAC GGCTTACCAG 
TCCTGTTTAC AGTACCCCTT CACCTCTGTG CCCTTGAGTG GGGGAGCCCC GGGTACCCGA 
GCCTCACGCA GGTGTGACCG AGCTGGCCGC TGGGAGCCAG GGGACTACTC GCACTGTCTG 
TACACCAATG ACATCACTCG GGTGCTCTAC ACCTTTGTGC TGATGCCCAT CAACGCCTCC 
AATGCATTGA CGTTGGCCCA CCAGCTGCGA GTGTATACCG CAGAGGCCGC CAGCTTCTCA 
GACATGATGG ACGTGGTCTA TGTGGCTCAG ATGATCCAGA AGTTTTTGGG TTACGTTGAC 
CAGATCAAGG AGCTGGTGGA GGTGATGGTG GACATGGCCA GCAACCTGAT GCTGGTGGAT 
GAGCACCTTC TGTGGCTGGC CCAGAGAGAA GACAAAGCCT GCAGTGGCAT TGTGGGTGCC 
CTGGAGCGAA TCGGAGGAGC TGCTCTTAGC CCCCATGCCC AGCACATCTC TGTGAATTCA 
AGAAATGTGG CACTGGAGGC CTACCTCATC AAGCCTCACA GCTACGTGGG TCTGACTTGC 
ACGGCTTTCC AGAGGAGGGA GGTAGGAGTG TCGGGTGCAC AGCCAAGCAG CGTCGGCCAG 
GATGCCCCAG TGGAGCCCGA ACCCCTAGCT GATCAGCAGC TTAGGTTCCG CTGCACCACT 
GGGAGGCCCA ACATTTCTCT GTCATCCTTC CACATCAAGA ATAGCGTGGC CCTGGCCTCC 
ATCCAGCTGC CCCCCAGCCT GTTCTCAACC CTTCCGGCTG CCCTGGCTCC CCCAGTCCCT 
CCAGATTGCA CCCTGCAACT GCTGGTCTTC AGAAATGGCC GTCTTTTCCG CAGCCACGGC 
AACAACACTT CCCGTCCTGG AGCAGCTGGG CCTGGCAAGA GGCGTGGTGT GGCCACCCCA 
GTCATATTTG CAGGAACCAG TGGCTGTGGT GTGGGAAACT TGACGGAGCC CGTGGCTGTG 
TCACTGAGGC ACTGGGCTGA AGGAGCTGAC CCCATGGCAG CTTGGTGGAA CCAGGACGGG 
CCTGGAGGCT GGAGTTCTGA AGGCTGCAGG CTCCGCTACA GCCAGCCCAA CGTCAGCTCC 
CTGTACTGCC AGCACTTGGG CAATGTGGCC GTGCTTATGG AGCTGAATGC ATTCCCGCGG 
GAGGCAGGAG GCTCTGGGGC TGGGTTGCAT CCCGTCGTGT ACCCCTGCAC GGCTTTGCTG 
CTACTCTGTC TCTTCTCCAC CATCATCACG TACATCCTCA ACCACAGCTC CATCCACGTG 
TCCCGGAAGG GTTGGCACAT GCTGCTGAAC CTTTGTTTCC ATATGGCCAT GACCTCTGCT 
GTCTTCGTGG GGGGCGTCAC GCTCACCAAC TACCAAATGG TTTGTCAAGC GGTGGGCATC 
ACTCTGCACT ACTCTTCCTT GTCGTCACTG CTCTGGATGG GGGTCAAGGC TCGAGTCCTC 
CACAAGGAGC TTAGTTGGAG GGCACCCCCT CTGGAAGAAG GGGAAGCAGC TCCGCCTGGT 
CCTCGCCCCA TGCTCCGTTG TTGGCTGGTG TGGCGTCCAA GCCTTGGTGC CTTCTACATA 
CCGGTGGCGT TGATTCTGCC TATCACCTGG ATCTACTTCT TGTGTGCAGG CCTTCACTTA 
CGGAGCCATG TGGCCCAGAA TCCAAAGCAG GGTAACAGGA TCTCTCTGGA GCCAGGGGAA 
GAGCTGAGGG GTTCCACCAG GCTCAGGAGT AGTGGCGTCC TCCTGAATGA CTCTGGTTCT 
CTTTTGGCTA CAGTTAGCGC AGGAGTAGGG ACACCTGCGC CCCCAGAGGA TGGTGATGGC 
GTATATTCTC CTGGAGTCCA GCTGGGGGCG CTGATGACCA CGCATTTCCT GTACCTGGCT 
ATGTGGGCTT GTGGTGCCTT GGCGGTGTCT CAGCGCTGGC TGCCCCGAGT GGTGTGTAGC 
TGTCTGTACG GCGTGGCAGC TTCAGCTCTT GGTCTGTTTG TCTTCACTCA CCACTGTGCC 
AGACGTAGAG ATGTCCGGGC TTCCTGGCGC GCCTGCTGCC CTCCTGCTTC GCCCTCGGCC 
TCCCATGTCC CAGCCCGGGC CCTGCCGACT GCTACAGAGG ATGGATCCCC AGTGTTGGGG 
GAGGGACCAG CCTCTCTCAA GTCCTCCCCA AGTGGCAGCA GTGGCCGCGC GCCGCCACCC 
CCCTGCAAAC TCACCAATCT GCAGGTGGCC CAGAGTCAGG TGTGCGAGGC AAGCGTGGCC 
GCCCGCGGAG ATGGAGAGCC AGAGCCCACC GGCTCCCGTG GCAGCCTAGC TCCCCGGCAC 
CATAACAACC TGCATCATGG GCGCCGAGTA CACAAGAGTC GGGCCAAGGG GCACCGAGCC 
GGAGAGACTG GTGGTAAGAG CCGGCTCAAG GCGTTGCGCG CGGGCACGTC CCCAGGAGCT 
CCCGAGCTGT TGTCCAGTGA GAGCGGCAGT TTGCACAACA GCCCGTCTGA CAGCTACCCG 
GGCAGCAGCC GCAACAGTCC AGGCGACGGT CTTCCACTCG AGGGTGAGCC CATGCTTACG 
CCGTCGGAGG GCAGTGACAC AAGCGCCGCG CCAATCGCTG AGACGGGGCG CCCCGGGCAG 
CGCCGCAGCG CCAGCCGTGA CAACCTCAAG GGCAGCGGCA GCGCGCTGGA GAGGGAGAGC 
AAGCGCCGCT CCTATCCGCT CAACACTACC AGTCTTAACG GTGCCCCCAA AGGGGGCAAG 
TATGAGGATG CCAGTGTAAC TGGTGCAGAG GCCATAGCGG GAGGCTCCAT GAAGACTGGC 
CTCTGGAAAA GCGAGACCAC CGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgra2 ( XM_006509223.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509286.3 Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X2, mRNA.

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CloneID	OMu82607
Clone ID Related Accession (Same CDS sequence)	XM_006509223.4 , XM_006509223.3
Accession Version	XM_006509223.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor A2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006509223.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCC GCCCCGCCCT CGGGCGCCCG GGACTCCCGC TGAGCACTCC TCCCGCACGC 
CCGGGTCCCT CCGGCCGGCG AGCCGTCCGG CCCCTGCGCT GTAGGTCCCC GCGGGGCGAT 
GGGTTGATGG GCGCGGGGGG ACGCAGGATG CCGGTGCCTC CCGCGCGTTT GCTGCTGCTG 
CCGCTGCTGC CTTGTCTTCT GCTCCTGGCT CCTGGAACTC GGGGTGCGCC TGGCTGCCCG 
GTCCCTATCC GCGGTTGCAA GTGCTCTGGG GAGCGGCCCA AGGGACTAAG TGGCGGCGCC 
CACAACCCGG CTCGAAGGAG GGTGGTGTGC GGCGGTGGGG ATCTCCCCGA ACCTCCAGAT 
CCCGGCCTTC TGCCAAACGG CACCATCACC TTGCTCTTGA GCAACAACAA GATTACTGGG 
CTCCGCAATG GATCCTTCTT GGGACTGTCC CTGTTGGAGA AGTTGGACCT GAGGAGCAAT 
GTCATCAGCA CTGTGCAGCC TGGAGCCTTC CTAGGTCTGG GAGAGCTAAA ACGCTTAGAT 
CTCTCCAACA ATCGGATTGG CTGTCTCACC TCTGAGACAT TTCAAGGGCT CCCTAGACTT 
CTCAGACTAA ACATATCTGG AAACATCTAC TCTAGTCTGC AACCTGGGGT CTTTGATGAG 
CTGCCAGCCC TTAAGATTGT GGACTTTGGT ACTGAGTTTC TGACCTGTGA CTGCCGCCTG 
CGCTGGCTGC TGCCCTGGGC CCGGAATCAC TCCCTGCAGC TGTCTGAGCG CACACTCTGT 
GCCTACCCCA GTGCCCTGCA CGCCCATGCC CTGAGCAGCC TCCAGGAGTC CCAGCTTCGC 
TGTGAAGGGG CCCTGGAACT GCACACCCAC TACCTCATCC CATCCCTTCG CCAAGTGGTG 
TTCCAGGGTG ACCGCCTGCC CTTCCAGTGC TCAGCCAGCT ACTTGGGCAA CGATACCCGG 
ATCCACTGGT ACCACAATGG GGCTCCTATG GAGAGCGATG AGCAGGCCGG CATCGTCCTT 
GCTGAAAACC TTATCCATGA TTGCACCTTC ATCACCAGTG AGCTGACCCT GTCTCACATT 
GGTGTGTGGG CCTCTGGTGA ATGGGAGTGC TCCGTGTCCA CAGTCCAAGG CAACACCAGC 
AAGAAGGTGG AGATAGTAGT GCTGGAGACC TCTGCCTCCT ACTGCCCTGC AGAGCGGGTG 
ACCAACAACC GTGGGGACTT CAGGTGGCCC CGAACCTTGG CTGGCATCAC GGCTTACCAG 
TCCTGTTTAC AGTACCCCTT CACCTCTGTG CCCTTGAGTG GGGGAGCCCC GGGTACCCGA 
GCCTCACGCA GGTGTGACCG AGCTGGCCGC TGGGAGCCAG GGGACTACTC GCACTGTCTG 
TACACCAATG ACATCACTCG GGTGCTCTAC ACCTTTGTGC TGATGCCCAT CAACGCCTCC 
AATGCATTGA CGTTGGCCCA CCAGCTGCGA GTGTATACCG CAGAGGCCGC CAGCTTCTCA 
GACATGATGG ACGTGGTCTA TGTGGCTCAG ATGATCCAGA AGTTTTTGGG TTACGTTGAC 
CAGATCAAGG AGCTGGTGGA GGTGATGGTG GACATGGCCA GCAACCTGAT GCTGGTGGAT 
GAGCACCTTC TGTGGCTGGC CCAGAGAGAA GACAAAGCCT GCAGTGGCAT TGTGGGTGCC 
CTGGAGCGAA TCGGAGGAGC TGCTCTTAGC CCCCATGCCC AGCACATCTC TGTGAATTCA 
AGAAATGTGG CACTGGAGGC CTACCTCATC AAGCCTCACA GCTACGTGGG TCTGACTTGC 
ACGGCTTTCC AGAGGAGGGA GGTAGGAGTG TCGGGTGCAC AGCCAAGCAG CGTCGGCCAG 
GATGCCCCAG TGGAGCCCGA ACCCCTAGCT GATCAGCAGC TTAGGTTCCG CTGCACCACT 
GGGAGGCCCA ACATTTCTCT GTCATCCTTC CACATCAAGA ATAGCGTGGC CCTGGCCTCC 
ATCCAGCTGC CCCCCAGCCT GTTCTCAACC CTTCCGGCTG CCCTGGCTCC CCCAGTCCCT 
CCAGATTGCA CCCTGCAACT GCTGGTCTTC AGAAATGGCC GTCTTTTCCG CAGCCACGGC 
AACAACACTT CCCGTCCTGG AGCAGCTGGG CCTGGCAAGA GGCGTGGTGT GGCCACCCCA 
GTCATATTTG CAGGAACCAG TGGCTGTGGT GTGGGAAACT TGACGGAGCC CGTGGCTGTG 
TCACTGAGGC ACTGGGCTGA AGGAGCTGAC CCCATGGCAG CTTGGTGGAA CCAGGACGGG 
CCTGGAGGCT GGAGTTCTGA AGGCTGCAGG CTCCGCTACA GCCAGCCCAA CGTCAGCTCC 
CTGTACTGCC AGCACTTGGG CAATGTGGCC GTGCTTATGG AGCTGAATGC ATTCCCGCGG 
GAGGCAGGAG GCTCTGGGGC TGGGTTGCAT CCCGTCGTGT ACCCCTGCAC GGCTTTGCTG 
CTACTCTGTC TCTTCTCCAC CATCATCACG TACATCCTCA ACCACAGCTC CATCCACGTG 
TCCCGGAAGG GTTGGCACAT GCTGCTGAAC CTTTGTTTCC ATATGGCCAT GACCTCTGCT 
GTCTTCGTGG GGGGCGTCAC GCTCACCAAC TACCAAATGG TTTGTCAAGC GGTGGGCATC 
ACTCTGCACT ACTCTTCCTT GTCGTCACTG CTCTGGATGG GGGTCAAGGC TCGAGTCCTC 
CACAAGGAGC TTAGTTGGAG GGCACCCCCT CTGGAAGAAG GGGAAGCAGC TCCGCCTGGT 
CCTCGCCCCA TGCTCCGGTT CTACTTGATT GCTGGAGGGA TCCCCCTCAT AATCTGCGGC 
ATCACCGCTG CGGTCAACAT CCACAACTAC CGGGACCACA GTCCCTATTG TTGGCTGGTG 
TGGCGTCCAA GCCTTGGTGC CTTCTACATA CCGGTGGCGT TGATTCTGCC TATCACCTGG 
ATCTACTTCT TGTGTGCAGG CCTTCACTTA CGGAGCCATG TGGCCCAGAA TCCAAAGCAG 
GTTAGCGCAG GAGTAGGGAC ACCTGCGCCC CCAGAGGATG GTGATGGCGT ATATTCTCCT 
GGAGTCCAGC TGGGGGCGCT GATGACCACG CATTTCCTGT ACCTGGCTAT GTGGGCTTGT 
GGTGCCTTGG CGGTGTCTCA GCGCTGGCTG CCCCGAGTGG TGTGTAGCTG TCTGTACGGC 
GTGGCAGCTT CAGCTCTTGG TCTGTTTGTC TTCACTCACC ACTGTGCCAG ACGTAGAGAT 
GTCCGGGCTT CCTGGCGCGC CTGCTGCCCT CCTGCTTCGC CCTCGGCCTC CCATGTCCCA 
GCCCGGGCCC TGCCGACTGC TACAGAGGAT GGATCCCCAG TGTTGGGGGA GGGACCAGCC 
TCTCTCAAGT CCTCCCCAAG TGGCAGCAGT GGCCGCGCGC CGCCACCCCC CTGCAAACTC 
ACCAATCTGC AGGTGGCCCA GAGTCAGGTG TGCGAGGCAA GCGTGGCCGC CCGCGGAGAT 
GGAGAGCCAG AGCCCACCGG CTCCCGTGGC AGCCTAGCTC CCCGGCACCA TAACAACCTG 
CATCATGGGC GCCGAGTACA CAAGAGTCGG GCCAAGGGGC ACCGAGCCGG AGAGACTGGT 
GGTAAGAGCC GGCTCAAGGC GTTGCGCGCG GGCACGTCCC CAGGAGCTCC CGAGCTGTTG 
TCCAGTGAGA GCGGCAGTTT GCACAACAGC CCGTCTGACA GCTACCCGGG CAGCAGCCGC 
AACAGTCCAG GCGACGGTCT TCCACTCGAG GGTGAGCCCA TGCTTACGCC GTCGGAGGGC 
AGTGACACAA GCGCCGCGCC AATCGCTGAG ACGGGGCGCC CCGGGCAGCG CCGCAGCGCC 
AGCCGTGACA ACCTCAAGGG CAGCGGCAGC GCGCTGGAGA GGGAGAGCAA GCGCCGCTCC 
TATCCGCTCA ACACTACCAG TCTTAACGGT GCCCCCAAAG GGGGCAAGTA TGAGGATGCC 
AGTGTAACTG GTGCAGAGGC CATAGCGGGA GGCTCCATGA AGACTGGCCT CTGGAAAAGC 
GAGACCACCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509286.3
CDS	301..4335
Translation	MAPRPALGRPGLPLSTPPARPGPSGRRAVRPLRCRSPRGDGLMGAGGRRMPVPPARLLLLPLLPCLLLLAPGTRGAPGCPVPIRGCKCSGERPKGLSGGAHNPARRRVVCGGGDLPEPPDPGLLPNGTITLLLSNNKITGLRNGSFLGLSLLEKLDLRSNVISTVQPGAFLGLGELKRLDLSNNRIGCLTSETFQGLPRLLRLNISGNIYSSLQPGVFDELPALKIVDFGTEFLTCDCRLRWLLPWARNHSLQLSERTLCAYPSALHAHALSSLQESQLRCEGALELHTHYLIPSLRQVVFQGDRLPFQCSASYLGNDTRIHWYHNGAPMESDEQAGIVLAENLIHDCTFITSELTLSHIGVWASGEWECSVSTVQGNTSKKVEIVVLETSASYCPAERVTNNRGDFRWPRTLAGITAYQSCLQYPFTSVPLSGGAPGTRASRRCDRAGRWEPGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDMASNLMLVDEHLLWLAQREDKACSGIVGALERIGGAALSPHAQHISVNSRNVALEAYLIKPHSYVGLTCTAFQRREVGVSGAQPSSVGQDAPVEPEPLADQQLRFRCTTGRPNISLSSFHIKNSVALASIQLPPSLFSTLPAALAPPVPPDCTLQLLVFRNGRLFRSHGNNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSLRHWAEGADPMAAWWNQDGPGGWSSEGCRLRYSQPNVSSLYCQHLGNVAVLMELNAFPREAGGSGAGLHPVVYPCTALLLLCLFSTIITYILNHSSIHVSRKGWHMLLNLCFHMAMTSAVFVGGVTLTNYQMVCQAVGITLHYSSLSSLLWMGVKARVLHKELSWRAPPLEEGEAAPPGPRPMLRFYLIAGGIPLIICGITAAVNIHNYRDHSPYCWLVWRPSLGAFYIPVALILPITWIYFLCAGLHLRSHVAQNPKQVSAGVGTPAPPEDGDGVYSPGVQLGALMTTHFLYLAMWACGALAVSQRWLPRVVCSCLYGVAASALGLFVFTHHCARRRDVRASWRACCPPASPSASHVPARALPTATEDGSPVLGEGPASLKSSPSGSSGRAPPPPCKLTNLQVAQSQVCEASVAARGDGEPEPTGSRGSLAPRHHNNLHHGRRVHKSRAKGHRAGETGGKSRLKALRAGTSPGAPELLSSESGSLHNSPSDSYPGSSRNSPGDGLPLEGEPMLTPSEGSDTSAAPIAETGRPGQRRSASRDNLKGSGSALERESKRRSYPLNTTSLNGAPKGGKYEDASVTGAEAIAGGSMKTGLWKSETTV

Target ORF information:

RefSeq Version	XM_006509223.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509223.4

ORF Insert Sequence:

ATGGCCCCCC GCCCCGCCCT CGGGCGCCCG GGACTCCCGC TGAGCACTCC TCCCGCACGC 
CCGGGTCCCT CCGGCCGGCG AGCCGTCCGG CCCCTGCGCT GTAGGTCCCC GCGGGGCGAT 
GGGTTGATGG GCGCGGGGGG ACGCAGGATG CCGGTGCCTC CCGCGCGTTT GCTGCTGCTG 
CCGCTGCTGC CTTGTCTTCT GCTCCTGGCT CCTGGAACTC GGGGTGCGCC TGGCTGCCCG 
GTCCCTATCC GCGGTTGCAA GTGCTCTGGG GAGCGGCCCA AGGGACTAAG TGGCGGCGCC 
CACAACCCGG CTCGAAGGAG GGTGGTGTGC GGCGGTGGGG ATCTCCCCGA ACCTCCAGAT 
CCCGGCCTTC TGCCAAACGG CACCATCACC TTGCTCTTGA GCAACAACAA GATTACTGGG 
CTCCGCAATG GATCCTTCTT GGGACTGTCC CTGTTGGAGA AGTTGGACCT GAGGAGCAAT 
GTCATCAGCA CTGTGCAGCC TGGAGCCTTC CTAGGTCTGG GAGAGCTAAA ACGCTTAGAT 
CTCTCCAACA ATCGGATTGG CTGTCTCACC TCTGAGACAT TTCAAGGGCT CCCTAGACTT 
CTCAGACTAA ACATATCTGG AAACATCTAC TCTAGTCTGC AACCTGGGGT CTTTGATGAG 
CTGCCAGCCC TTAAGATTGT GGACTTTGGT ACTGAGTTTC TGACCTGTGA CTGCCGCCTG 
CGCTGGCTGC TGCCCTGGGC CCGGAATCAC TCCCTGCAGC TGTCTGAGCG CACACTCTGT 
GCCTACCCCA GTGCCCTGCA CGCCCATGCC CTGAGCAGCC TCCAGGAGTC CCAGCTTCGC 
TGTGAAGGGG CCCTGGAACT GCACACCCAC TACCTCATCC CATCCCTTCG CCAAGTGGTG 
TTCCAGGGTG ACCGCCTGCC CTTCCAGTGC TCAGCCAGCT ACTTGGGCAA CGATACCCGG 
ATCCACTGGT ACCACAATGG GGCTCCTATG GAGAGCGATG AGCAGGCCGG CATCGTCCTT 
GCTGAAAACC TTATCCATGA TTGCACCTTC ATCACCAGTG AGCTGACCCT GTCTCACATT 
GGTGTGTGGG CCTCTGGTGA ATGGGAGTGC TCCGTGTCCA CAGTCCAAGG CAACACCAGC 
AAGAAGGTGG AGATAGTAGT GCTGGAGACC TCTGCCTCCT ACTGCCCTGC AGAGCGGGTG 
ACCAACAACC GTGGGGACTT CAGGTGGCCC CGAACCTTGG CTGGCATCAC GGCTTACCAG 
TCCTGTTTAC AGTACCCCTT CACCTCTGTG CCCTTGAGTG GGGGAGCCCC GGGTACCCGA 
GCCTCACGCA GGTGTGACCG AGCTGGCCGC TGGGAGCCAG GGGACTACTC GCACTGTCTG 
TACACCAATG ACATCACTCG GGTGCTCTAC ACCTTTGTGC TGATGCCCAT CAACGCCTCC 
AATGCATTGA CGTTGGCCCA CCAGCTGCGA GTGTATACCG CAGAGGCCGC CAGCTTCTCA 
GACATGATGG ACGTGGTCTA TGTGGCTCAG ATGATCCAGA AGTTTTTGGG TTACGTTGAC 
CAGATCAAGG AGCTGGTGGA GGTGATGGTG GACATGGCCA GCAACCTGAT GCTGGTGGAT 
GAGCACCTTC TGTGGCTGGC CCAGAGAGAA GACAAAGCCT GCAGTGGCAT TGTGGGTGCC 
CTGGAGCGAA TCGGAGGAGC TGCTCTTAGC CCCCATGCCC AGCACATCTC TGTGAATTCA 
AGAAATGTGG CACTGGAGGC CTACCTCATC AAGCCTCACA GCTACGTGGG TCTGACTTGC 
ACGGCTTTCC AGAGGAGGGA GGTAGGAGTG TCGGGTGCAC AGCCAAGCAG CGTCGGCCAG 
GATGCCCCAG TGGAGCCCGA ACCCCTAGCT GATCAGCAGC TTAGGTTCCG CTGCACCACT 
GGGAGGCCCA ACATTTCTCT GTCATCCTTC CACATCAAGA ATAGCGTGGC CCTGGCCTCC 
ATCCAGCTGC CCCCCAGCCT GTTCTCAACC CTTCCGGCTG CCCTGGCTCC CCCAGTCCCT 
CCAGATTGCA CCCTGCAACT GCTGGTCTTC AGAAATGGCC GTCTTTTCCG CAGCCACGGC 
AACAACACTT CCCGTCCTGG AGCAGCTGGG CCTGGCAAGA GGCGTGGTGT GGCCACCCCA 
GTCATATTTG CAGGAACCAG TGGCTGTGGT GTGGGAAACT TGACGGAGCC CGTGGCTGTG 
TCACTGAGGC ACTGGGCTGA AGGAGCTGAC CCCATGGCAG CTTGGTGGAA CCAGGACGGG 
CCTGGAGGCT GGAGTTCTGA AGGCTGCAGG CTCCGCTACA GCCAGCCCAA CGTCAGCTCC 
CTGTACTGCC AGCACTTGGG CAATGTGGCC GTGCTTATGG AGCTGAATGC ATTCCCGCGG 
GAGGCAGGAG GCTCTGGGGC TGGGTTGCAT CCCGTCGTGT ACCCCTGCAC GGCTTTGCTG 
CTACTCTGTC TCTTCTCCAC CATCATCACG TACATCCTCA ACCACAGCTC CATCCACGTG 
TCCCGGAAGG GTTGGCACAT GCTGCTGAAC CTTTGTTTCC ATATGGCCAT GACCTCTGCT 
GTCTTCGTGG GGGGCGTCAC GCTCACCAAC TACCAAATGG TTTGTCAAGC GGTGGGCATC 
ACTCTGCACT ACTCTTCCTT GTCGTCACTG CTCTGGATGG GGGTCAAGGC TCGAGTCCTC 
CACAAGGAGC TTAGTTGGAG GGCACCCCCT CTGGAAGAAG GGGAAGCAGC TCCGCCTGGT 
CCTCGCCCCA TGCTCCGGTT CTACTTGATT GCTGGAGGGA TCCCCCTCAT AATCTGCGGC 
ATCACCGCTG CGGTCAACAT CCACAACTAC CGGGACCACA GTCCCTATTG TTGGCTGGTG 
TGGCGTCCAA GCCTTGGTGC CTTCTACATA CCGGTGGCGT TGATTCTGCC TATCACCTGG 
ATCTACTTCT TGTGTGCAGG CCTTCACTTA CGGAGCCATG TGGCCCAGAA TCCAAAGCAG 
GTTAGCGCAG GAGTAGGGAC ACCTGCGCCC CCAGAGGATG GTGATGGCGT ATATTCTCCT 
GGAGTCCAGC TGGGGGCGCT GATGACCACG CATTTCCTGT ACCTGGCTAT GTGGGCTTGT 
GGTGCCTTGG CGGTGTCTCA GCGCTGGCTG CCCCGAGTGG TGTGTAGCTG TCTGTACGGC 
GTGGCAGCTT CAGCTCTTGG TCTGTTTGTC TTCACTCACC ACTGTGCCAG ACGTAGAGAT 
GTCCGGGCTT CCTGGCGCGC CTGCTGCCCT CCTGCTTCGC CCTCGGCCTC CCATGTCCCA 
GCCCGGGCCC TGCCGACTGC TACAGAGGAT GGATCCCCAG TGTTGGGGGA GGGACCAGCC 
TCTCTCAAGT CCTCCCCAAG TGGCAGCAGT GGCCGCGCGC CGCCACCCCC CTGCAAACTC 
ACCAATCTGC AGGTGGCCCA GAGTCAGGTG TGCGAGGCAA GCGTGGCCGC CCGCGGAGAT 
GGAGAGCCAG AGCCCACCGG CTCCCGTGGC AGCCTAGCTC CCCGGCACCA TAACAACCTG 
CATCATGGGC GCCGAGTACA CAAGAGTCGG GCCAAGGGGC ACCGAGCCGG AGAGACTGGT 
GGTAAGAGCC GGCTCAAGGC GTTGCGCGCG GGCACGTCCC CAGGAGCTCC CGAGCTGTTG 
TCCAGTGAGA GCGGCAGTTT GCACAACAGC CCGTCTGACA GCTACCCGGG CAGCAGCCGC 
AACAGTCCAG GCGACGGTCT TCCACTCGAG GGTGAGCCCA TGCTTACGCC GTCGGAGGGC 
AGTGACACAA GCGCCGCGCC AATCGCTGAG ACGGGGCGCC CCGGGCAGCG CCGCAGCGCC 
AGCCGTGACA ACCTCAAGGG CAGCGGCAGC GCGCTGGAGA GGGAGAGCAA GCGCCGCTCC 
TATCCGCTCA ACACTACCAG TCTTAACGGT GCCCCCAAAG GGGGCAAGTA TGAGGATGCC 
AGTGTAACTG GTGCAGAGGC CATAGCGGGA GGCTCCATGA AGACTGGCCT CTGGAAAAGC 
GAGACCACCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgra2 ( NM_054044.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_473385.2 Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), mRNA.

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CloneID	OMu13324
Clone ID Related Accession (Same CDS sequence)	NM_054044.2
Accession Version	NM_054044.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor A2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF378759.1 and CJ243065.1. On Jul 21, 2010 this sequence version replaced NM_054044.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF378759.1, BC138451.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGCGG GGGGACGCAG GATGCCGGTG CCTCCCGCGC GTTTGCTGCT GCTGCCGCTG 
CTGCCTTGTC TTCTGCTCCT GGCTCCTGGA ACTCGGGGTG CGCCTGGCTG CCCGGTCCCT 
ATCCGCGGTT GCAAGTGCTC TGGGGAGCGG CCCAAGGGAC TAAGTGGCGG CGCCCACAAC 
CCGGCTCGAA GGAGGGTGGT GTGCGGCGGT GGGGATCTCC CCGAACCTCC AGATCCCGGC 
CTTCTGCCAA ACGGCACCAT CACCTTGCTC TTGAGCAACA ACAAGATTAC TGGGCTCCGC 
AATGGATCCT TCTTGGGACT GTCCCTGTTG GAGAAGTTGG ACCTGAGGAG CAATGTCATC 
AGCACTGTGC AGCCTGGAGC CTTCCTAGGT CTGGGAGAGC TAAAACGCTT AGATCTCTCC 
AACAATCGGA TTGGCTGTCT CACCTCTGAG ACATTTCAAG GGCTCCCTAG ACTTCTCAGA 
CTAAACATAT CTGGAAACAT CTACTCTAGT CTGCAACCTG GGGTCTTTGA TGAGCTGCCA 
GCCCTTAAGA TTGTGGACTT TGGTACTGAG TTTCTGACCT GTGACTGCCG CCTGCGCTGG 
CTGCTGCCCT GGGCCCGGAA TCACTCCCTG CAGCTGTCTG AGCGCACACT CTGTGCCTAC 
CCCAGTGCCC TGCACGCCCA TGCCCTGAGC AGCCTCCAGG AGTCCCAGCT TCGCTGTGAA 
GGGGCCCTGG AACTGCACAC CCACTACCTC ATCCCATCCC TTCGCCAAGT GGTGTTCCAG 
GGTGACCGCC TGCCCTTCCA GTGCTCAGCC AGCTACTTGG GCAACGATAC CCGGATCCAC 
TGGTACCACA ATGGGGCTCC TATGGAGAGC GATGAGCAGG CCGGCATCGT CCTTGCTGAA 
AACCTTATCC ATGATTGCAC CTTCATCACC AGTGAGCTGA CCCTGTCTCA CATTGGTGTG 
TGGGCCTCTG GTGAATGGGA GTGCTCCGTG TCCACAGTCC AAGGCAACAC CAGCAAGAAG 
GTGGAGATAG TAGTGCTGGA GACCTCTGCC TCCTACTGCC CTGCAGAGCG GGTGACCAAC 
AACCGTGGGG ACTTCAGGTG GCCCCGAACC TTGGCTGGCA TCACGGCTTA CCAGTCCTGT 
TTACAGTACC CCTTCACCTC TGTGCCCTTG AGTGGGGGAG CCCCGGGTAC CCGAGCCTCA 
CGCAGGTGTG ACCGAGCTGG CCGCTGGGAG CCAGGGGACT ACTCGCACTG TCTGTACACC 
AATGACATCA CTCGGGTGCT CTACACCTTT GTGCTGATGC CCATCAACGC CTCCAATGCA 
TTGACGTTGG CCCACCAGCT GCGAGTGTAT ACCGCAGAGG CCGCCAGCTT CTCAGACATG 
ATGGACGTGG TCTATGTGGC TCAGATGATC CAGAAGTTTT TGGGTTACGT TGACCAGATC 
AAGGAGCTGG TGGAGGTGAT GGTGGACATG GCCAGCAACC TGATGCTGGT GGATGAGCAC 
CTTCTGTGGC TGGCCCAGAG AGAAGACAAA GCCTGCAGTG GCATTGTGGG TGCCCTGGAG 
CGAATCGGAG GAGCTGCTCT TAGCCCCCAT GCCCAGCACA TCTCTGTGAA TTCAAGAAAT 
GTGGCACTGG AGGCCTACCT CATCAAGCCT CACAGCTACG TGGGTCTGAC TTGCACGGCT 
TTCCAGAGGA GGGAGGTAGG AGTGTCGGGT GCACAGCCAA GCAGCGTCGG CCAGGATGCC 
CCAGTGGAGC CCGAACCCCT AGCTGATCAG CAGCTTAGGT TCCGCTGCAC CACTGGGAGG 
CCCAACATTT CTCTGTCATC CTTCCACATC AAGAATAGCG TGGCCCTGGC CTCCATCCAG 
CTGCCCCCCA GCCTGTTCTC AACCCTTCCG GCTGCCCTGG CTCCCCCAGT CCCTCCAGAT 
TGCACCCTGC AACTGCTGGT CTTCAGAAAT GGCCGTCTTT TCCGCAGCCA CGGCAACAAC 
ACTTCCCGTC CTGGAGCAGC TGGGCCTGGC AAGAGGCGTG GTGTGGCCAC CCCAGTCATA 
TTTGCAGGAA CCAGTGGCTG TGGTGTGGGA AACTTGACGG AGCCCGTGGC TGTGTCACTG 
AGGCACTGGG CTGAAGGAGC TGACCCCATG GCAGCTTGGT GGAACCAGGA CGGGCCTGGA 
GGCTGGAGTT CTGAAGGCTG CAGGCTCCGC TACAGCCAGC CCAACGTCAG CTCCCTGTAC 
TGCCAGCACT TGGGCAATGT GGCCGTGCTT ATGGAGCTGA ATGCATTCCC GCGGGAGGCA 
GGAGGCTCTG GGGCTGGGTT GCATCCCGTC GTGTACCCCT GCACGGCTTT GCTGCTACTC 
TGTCTCTTCT CCACCATCAT CACGTACATC CTCAACCACA GCTCCATCCA CGTGTCCCGG 
AAGGGTTGGC ACATGCTGCT GAACCTTTGT TTCCATATGG CCATGACCTC TGCTGTCTTC 
GTGGGGGGCG TCACGCTCAC CAACTACCAA ATGGTTTGTC AAGCGGTGGG CATCACTCTG 
CACTACTCTT CCTTGTCGTC ACTGCTCTGG ATGGGGGTCA AGGCTCGAGT CCTCCACAAG 
GAGCTTAGTT GGAGGGCACC CCCTCTGGAA GAAGGGGAAG CAGCTCCGCC TGGTCCTCGC 
CCCATGCTCC GGTTCTACTT GATTGCTGGA GGGATCCCCC TCATAATCTG CGGCATCACC 
GCTGCGGTCA ACATCCACAA CTACCGGGAC CACAGTCCCT ATTGTTGGCT GGTGTGGCGT 
CCAAGCCTTG GTGCCTTCTA CATACCGGTG GCGTTGATTC TGCCTATCAC CTGGATCTAC 
TTCTTGTGTG CAGGCCTTCA CTTACGGAGC CATGTGGCCC AGAATCCAAA GCAGGGTAAC 
AGGATCTCTC TGGAGCCAGG GGAAGAGCTG AGGGGTTCCA CCAGGCTCAG GAGTAGTGGC 
GTCCTCCTGA ATGACTCTGG TTCTCTTTTG GCTACAGTTA GCGCAGGAGT AGGGACACCT 
GCGCCCCCAG AGGATGGTGA TGGCGTATAT TCTCCTGGAG TCCAGCTGGG GGCGCTGATG 
ACCACGCATT TCCTGTACCT GGCTATGTGG GCTTGTGGTG CCTTGGCGGT GTCTCAGCGC 
TGGCTGCCCC GAGTGGTGTG TAGCTGTCTG TACGGCGTGG CAGCTTCAGC TCTTGGTCTG 
TTTGTCTTCA CTCACCACTG TGCCAGACGT AGAGATGTCC GGGCTTCCTG GCGCGCCTGC 
TGCCCTCCTG CTTCGCCCTC GGCCTCCCAT GTCCCAGCCC GGGCCCTGCC GACTGCTACA 
GAGGATGGAT CCCCAGTGTT GGGGGAGGGA CCAGCCTCTC TCAAGTCCTC CCCAAGTGGC 
AGCAGTGGCC GCGCGCCGCC ACCCCCCTGC AAACTCACCA ATCTGCAGGT GGCCCAGAGT 
CAGGTGTGCG AGGCAAGCGT GGCCGCCCGC GGAGATGGAG AGCCAGAGCC CACCGGCTCC 
CGTGGCAGCC TAGCTCCCCG GCACCATAAC AACCTGCATC ATGGGCGCCG AGTACACAAG 
AGTCGGGCCA AGGGGCACCG AGCCGGAGAG ACTGGTGGTA AGAGCCGGCT CAAGGCGTTG 
CGCGCGGGCA CGTCCCCAGG AGCTCCCGAG CTGTTGTCCA GTGAGAGCGG CAGTTTGCAC 
AACAGCCCGT CTGACAGCTA CCCGGGCAGC AGCCGCAACA GTCCAGGCGA CGGTCTTCCA 
CTCGAGGGTG AGCCCATGCT TACGCCGTCG GAGGGCAGTG ACACAAGCGC CGCGCCAATC 
GCTGAGACGG GGCGCCCCGG GCAGCGCCGC AGCGCCAGCC GTGACAACCT CAAGGGCAGC 
GGCAGCGCGC TGGAGAGGGA GAGCAAGCGC CGCTCCTATC CGCTCAACAC TACCAGTCTT 
AACGGTGCCC CCAAAGGGGG CAAGTATGAG GATGCCAGTG TAACTGGTGC AGAGGCCATA 
GCGGGAGGCT CCATGAAGAC TGGCCTCTGG AAAAGCGAGA CCACCGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_473385.2
CDS	106..4116
Translation	MGAGGRRMPVPPARLLLLPLLPCLLLLAPGTRGAPGCPVPIRGCKCSGERPKGLSGGAHNPARRRVVCGGGDLPEPPDPGLLPNGTITLLLSNNKITGLRNGSFLGLSLLEKLDLRSNVISTVQPGAFLGLGELKRLDLSNNRIGCLTSETFQGLPRLLRLNISGNIYSSLQPGVFDELPALKIVDFGTEFLTCDCRLRWLLPWARNHSLQLSERTLCAYPSALHAHALSSLQESQLRCEGALELHTHYLIPSLRQVVFQGDRLPFQCSASYLGNDTRIHWYHNGAPMESDEQAGIVLAENLIHDCTFITSELTLSHIGVWASGEWECSVSTVQGNTSKKVEIVVLETSASYCPAERVTNNRGDFRWPRTLAGITAYQSCLQYPFTSVPLSGGAPGTRASRRCDRAGRWEPGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDMASNLMLVDEHLLWLAQREDKACSGIVGALERIGGAALSPHAQHISVNSRNVALEAYLIKPHSYVGLTCTAFQRREVGVSGAQPSSVGQDAPVEPEPLADQQLRFRCTTGRPNISLSSFHIKNSVALASIQLPPSLFSTLPAALAPPVPPDCTLQLLVFRNGRLFRSHGNNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSLRHWAEGADPMAAWWNQDGPGGWSSEGCRLRYSQPNVSSLYCQHLGNVAVLMELNAFPREAGGSGAGLHPVVYPCTALLLLCLFSTIITYILNHSSIHVSRKGWHMLLNLCFHMAMTSAVFVGGVTLTNYQMVCQAVGITLHYSSLSSLLWMGVKARVLHKELSWRAPPLEEGEAAPPGPRPMLRFYLIAGGIPLIICGITAAVNIHNYRDHSPYCWLVWRPSLGAFYIPVALILPITWIYFLCAGLHLRSHVAQNPKQGNRISLEPGEELRGSTRLRSSGVLLNDSGSLLATVSAGVGTPAPPEDGDGVYSPGVQLGALMTTHFLYLAMWACGALAVSQRWLPRVVCSCLYGVAASALGLFVFTHHCARRRDVRASWRACCPPASPSASHVPARALPTATEDGSPVLGEGPASLKSSPSGSSGRAPPPPCKLTNLQVAQSQVCEASVAARGDGEPEPTGSRGSLAPRHHNNLHHGRRVHKSRAKGHRAGETGGKSRLKALRAGTSPGAPELLSSESGSLHNSPSDSYPGSSRNSPGDGLPLEGEPMLTPSEGSDTSAAPIAETGRPGQRRSASRDNLKGSGSALERESKRRSYPLNTTSLNGAPKGGKYEDASVTGAEAIAGGSMKTGLWKSETTV

Target ORF information:

RefSeq Version	NM_054044.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_054044.2

ORF Insert Sequence:

ATGGGCGCGG GGGGACGCAG GATGCCGGTG CCTCCCGCGC GTTTGCTGCT GCTGCCGCTG 
CTGCCTTGTC TTCTGCTCCT GGCTCCTGGA ACTCGGGGTG CGCCTGGCTG CCCGGTCCCT 
ATCCGCGGTT GCAAGTGCTC TGGGGAGCGG CCCAAGGGAC TAAGTGGCGG CGCCCACAAC 
CCGGCTCGAA GGAGGGTGGT GTGCGGCGGT GGGGATCTCC CCGAACCTCC AGATCCCGGC 
CTTCTGCCAA ACGGCACCAT CACCTTGCTC TTGAGCAACA ACAAGATTAC TGGGCTCCGC 
AATGGATCCT TCTTGGGACT GTCCCTGTTG GAGAAGTTGG ACCTGAGGAG CAATGTCATC 
AGCACTGTGC AGCCTGGAGC CTTCCTAGGT CTGGGAGAGC TAAAACGCTT AGATCTCTCC 
AACAATCGGA TTGGCTGTCT CACCTCTGAG ACATTTCAAG GGCTCCCTAG ACTTCTCAGA 
CTAAACATAT CTGGAAACAT CTACTCTAGT CTGCAACCTG GGGTCTTTGA TGAGCTGCCA 
GCCCTTAAGA TTGTGGACTT TGGTACTGAG TTTCTGACCT GTGACTGCCG CCTGCGCTGG 
CTGCTGCCCT GGGCCCGGAA TCACTCCCTG CAGCTGTCTG AGCGCACACT CTGTGCCTAC 
CCCAGTGCCC TGCACGCCCA TGCCCTGAGC AGCCTCCAGG AGTCCCAGCT TCGCTGTGAA 
GGGGCCCTGG AACTGCACAC CCACTACCTC ATCCCATCCC TTCGCCAAGT GGTGTTCCAG 
GGTGACCGCC TGCCCTTCCA GTGCTCAGCC AGCTACTTGG GCAACGATAC CCGGATCCAC 
TGGTACCACA ATGGGGCTCC TATGGAGAGC GATGAGCAGG CCGGCATCGT CCTTGCTGAA 
AACCTTATCC ATGATTGCAC CTTCATCACC AGTGAGCTGA CCCTGTCTCA CATTGGTGTG 
TGGGCCTCTG GTGAATGGGA GTGCTCCGTG TCCACAGTCC AAGGCAACAC CAGCAAGAAG 
GTGGAGATAG TAGTGCTGGA GACCTCTGCC TCCTACTGCC CTGCAGAGCG GGTGACCAAC 
AACCGTGGGG ACTTCAGGTG GCCCCGAACC TTGGCTGGCA TCACGGCTTA CCAGTCCTGT 
TTACAGTACC CCTTCACCTC TGTGCCCTTG AGTGGGGGAG CCCCGGGTAC CCGAGCCTCA 
CGCAGGTGTG ACCGAGCTGG CCGCTGGGAG CCAGGGGACT ACTCGCACTG TCTGTACACC 
AATGACATCA CTCGGGTGCT CTACACCTTT GTGCTGATGC CCATCAACGC CTCCAATGCA 
TTGACGTTGG CCCACCAGCT GCGAGTGTAT ACCGCAGAGG CCGCCAGCTT CTCAGACATG 
ATGGACGTGG TCTATGTGGC TCAGATGATC CAGAAGTTTT TGGGTTACGT TGACCAGATC 
AAGGAGCTGG TGGAGGTGAT GGTGGACATG GCCAGCAACC TGATGCTGGT GGATGAGCAC 
CTTCTGTGGC TGGCCCAGAG AGAAGACAAA GCCTGCAGTG GCATTGTGGG TGCCCTGGAG 
CGAATCGGAG GAGCTGCTCT TAGCCCCCAT GCCCAGCACA TCTCTGTGAA TTCAAGAAAT 
GTGGCACTGG AGGCCTACCT CATCAAGCCT CACAGCTACG TGGGTCTGAC TTGCACGGCT 
TTCCAGAGGA GGGAGGTAGG AGTGTCGGGT GCACAGCCAA GCAGCGTCGG CCAGGATGCC 
CCAGTGGAGC CCGAACCCCT AGCTGATCAG CAGCTTAGGT TCCGCTGCAC CACTGGGAGG 
CCCAACATTT CTCTGTCATC CTTCCACATC AAGAATAGCG TGGCCCTGGC CTCCATCCAG 
CTGCCCCCCA GCCTGTTCTC AACCCTTCCG GCTGCCCTGG CTCCCCCAGT CCCTCCAGAT 
TGCACCCTGC AACTGCTGGT CTTCAGAAAT GGCCGTCTTT TCCGCAGCCA CGGCAACAAC 
ACTTCCCGTC CTGGAGCAGC TGGGCCTGGC AAGAGGCGTG GTGTGGCCAC CCCAGTCATA 
TTTGCAGGAA CCAGTGGCTG TGGTGTGGGA AACTTGACGG AGCCCGTGGC TGTGTCACTG 
AGGCACTGGG CTGAAGGAGC TGACCCCATG GCAGCTTGGT GGAACCAGGA CGGGCCTGGA 
GGCTGGAGTT CTGAAGGCTG CAGGCTCCGC TACAGCCAGC CCAACGTCAG CTCCCTGTAC 
TGCCAGCACT TGGGCAATGT GGCCGTGCTT ATGGAGCTGA ATGCATTCCC GCGGGAGGCA 
GGAGGCTCTG GGGCTGGGTT GCATCCCGTC GTGTACCCCT GCACGGCTTT GCTGCTACTC 
TGTCTCTTCT CCACCATCAT CACGTACATC CTCAACCACA GCTCCATCCA CGTGTCCCGG 
AAGGGTTGGC ACATGCTGCT GAACCTTTGT TTCCATATGG CCATGACCTC TGCTGTCTTC 
GTGGGGGGCG TCACGCTCAC CAACTACCAA ATGGTTTGTC AAGCGGTGGG CATCACTCTG 
CACTACTCTT CCTTGTCGTC ACTGCTCTGG ATGGGGGTCA AGGCTCGAGT CCTCCACAAG 
GAGCTTAGTT GGAGGGCACC CCCTCTGGAA GAAGGGGAAG CAGCTCCGCC TGGTCCTCGC 
CCCATGCTCC GGTTCTACTT GATTGCTGGA GGGATCCCCC TCATAATCTG CGGCATCACC 
GCTGCGGTCA ACATCCACAA CTACCGGGAC CACAGTCCCT ATTGTTGGCT GGTGTGGCGT 
CCAAGCCTTG GTGCCTTCTA CATACCGGTG GCGTTGATTC TGCCTATCAC CTGGATCTAC 
TTCTTGTGTG CAGGCCTTCA CTTACGGAGC CATGTGGCCC AGAATCCAAA GCAGGGTAAC 
AGGATCTCTC TGGAGCCAGG GGAAGAGCTG AGGGGTTCCA CCAGGCTCAG GAGTAGTGGC 
GTCCTCCTGA ATGACTCTGG TTCTCTTTTG GCTACAGTTA GCGCAGGAGT AGGGACACCT 
GCGCCCCCAG AGGATGGTGA TGGCGTATAT TCTCCTGGAG TCCAGCTGGG GGCGCTGATG 
ACCACGCATT TCCTGTACCT GGCTATGTGG GCTTGTGGTG CCTTGGCGGT GTCTCAGCGC 
TGGCTGCCCC GAGTGGTGTG TAGCTGTCTG TACGGCGTGG CAGCTTCAGC TCTTGGTCTG 
TTTGTCTTCA CTCACCACTG TGCCAGACGT AGAGATGTCC GGGCTTCCTG GCGCGCCTGC 
TGCCCTCCTG CTTCGCCCTC GGCCTCCCAT GTCCCAGCCC GGGCCCTGCC GACTGCTACA 
GAGGATGGAT CCCCAGTGTT GGGGGAGGGA CCAGCCTCTC TCAAGTCCTC CCCAAGTGGC 
AGCAGTGGCC GCGCGCCGCC ACCCCCCTGC AAACTCACCA ATCTGCAGGT GGCCCAGAGT 
CAGGTGTGCG AGGCAAGCGT GGCCGCCCGC GGAGATGGAG AGCCAGAGCC CACCGGCTCC 
CGTGGCAGCC TAGCTCCCCG GCACCATAAC AACCTGCATC ATGGGCGCCG AGTACACAAG 
AGTCGGGCCA AGGGGCACCG AGCCGGAGAG ACTGGTGGTA AGAGCCGGCT CAAGGCGTTG 
CGCGCGGGCA CGTCCCCAGG AGCTCCCGAG CTGTTGTCCA GTGAGAGCGG CAGTTTGCAC 
AACAGCCCGT CTGACAGCTA CCCGGGCAGC AGCCGCAACA GTCCAGGCGA CGGTCTTCCA 
CTCGAGGGTG AGCCCATGCT TACGCCGTCG GAGGGCAGTG ACACAAGCGC CGCGCCAATC 
GCTGAGACGG GGCGCCCCGG GCAGCGCCGC AGCGCCAGCC GTGACAACCT CAAGGGCAGC 
GGCAGCGCGC TGGAGAGGGA GAGCAAGCGC CGCTCCTATC CGCTCAACAC TACCAGTCTT 
AACGGTGCCC CCAAAGGGGG CAAGTATGAG GATGCCAGTG TAACTGGTGC AGAGGCCATA 
GCGGGAGGCT CCATGAAGAC TGGCCTCTGG AAAAGCGAGA CCACCGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgra2 ( XM_006509222.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509285.3 Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X1, mRNA.

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CloneID	OMu82606
Clone ID Related Accession (Same CDS sequence)	XM_006509222.3 , XM_006509222.4
Accession Version	XM_006509222.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor A2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509222.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCC GCCCCGCCCT CGGGCGCCCG GGACTCCCGC TGAGCACTCC TCCCGCACGC 
CCGGGTCCCT CCGGCCGGCG AGCCGTCCGG CCCCTGCGCT GTAGGTCCCC GCGGGGCGAT 
GGGTTGATGG GCGCGGGGGG ACGCAGGATG CCGGTGCCTC CCGCGCGTTT GCTGCTGCTG 
CCGCTGCTGC CTTGTCTTCT GCTCCTGGCT CCTGGAACTC GGGGTGCGCC TGGCTGCCCG 
GTCCCTATCC GCGGTTGCAA GTGCTCTGGG GAGCGGCCCA AGGGACTAAG TGGCGGCGCC 
CACAACCCGG CTCGAAGGAG GGTGGTGTGC GGCGGTGGGG ATCTCCCCGA ACCTCCAGAT 
CCCGGCCTTC TGCCAAACGG CACCATCACC TTGCTCTTGA GCAACAACAA GATTACTGGG 
CTCCGCAATG GATCCTTCTT GGGACTGTCC CTGTTGGAGA AGTTGGACCT GAGGAGCAAT 
GTCATCAGCA CTGTGCAGCC TGGAGCCTTC CTAGGTCTGG GAGAGCTAAA ACGCTTAGAT 
CTCTCCAACA ATCGGATTGG CTGTCTCACC TCTGAGACAT TTCAAGGGCT CCCTAGACTT 
CTCAGACTAA ACATATCTGG AAACATCTAC TCTAGTCTGC AACCTGGGGT CTTTGATGAG 
CTGCCAGCCC TTAAGATTGT GGACTTTGGT ACTGAGTTTC TGACCTGTGA CTGCCGCCTG 
CGCTGGCTGC TGCCCTGGGC CCGGAATCAC TCCCTGCAGC TGTCTGAGCG CACACTCTGT 
GCCTACCCCA GTGCCCTGCA CGCCCATGCC CTGAGCAGCC TCCAGGAGTC CCAGCTTCGC 
TGTGAAGGGG CCCTGGAACT GCACACCCAC TACCTCATCC CATCCCTTCG CCAAGTGGTG 
TTCCAGGGTG ACCGCCTGCC CTTCCAGTGC TCAGCCAGCT ACTTGGGCAA CGATACCCGG 
ATCCACTGGT ACCACAATGG GGCTCCTATG GAGAGCGATG AGCAGGCCGG CATCGTCCTT 
GCTGAAAACC TTATCCATGA TTGCACCTTC ATCACCAGTG AGCTGACCCT GTCTCACATT 
GGTGTGTGGG CCTCTGGTGA ATGGGAGTGC TCCGTGTCCA CAGTCCAAGG CAACACCAGC 
AAGAAGGTGG AGATAGTAGT GCTGGAGACC TCTGCCTCCT ACTGCCCTGC AGAGCGGGTG 
ACCAACAACC GTGGGGACTT CAGGTGGCCC CGAACCTTGG CTGGCATCAC GGCTTACCAG 
TCCTGTTTAC AGTACCCCTT CACCTCTGTG CCCTTGAGTG GGGGAGCCCC GGGTACCCGA 
GCCTCACGCA GGTGTGACCG AGCTGGCCGC TGGGAGCCAG GGGACTACTC GCACTGTCTG 
TACACCAATG ACATCACTCG GGTGCTCTAC ACCTTTGTGC TGATGCCCAT CAACGCCTCC 
AATGCATTGA CGTTGGCCCA CCAGCTGCGA GTGTATACCG CAGAGGCCGC CAGCTTCTCA 
GACATGATGG ACGTGGTCTA TGTGGCTCAG ATGATCCAGA AGTTTTTGGG TTACGTTGAC 
CAGATCAAGG AGCTGGTGGA GGTGATGGTG GACATGGCCA GCAACCTGAT GCTGGTGGAT 
GAGCACCTTC TGTGGCTGGC CCAGAGAGAA GACAAAGCCT GCAGTGGCAT TGTGGGTGCC 
CTGGAGCGAA TCGGAGGAGC TGCTCTTAGC CCCCATGCCC AGCACATCTC TGTGAATTCA 
AGAAATGTGG CACTGGAGGC CTACCTCATC AAGCCTCACA GCTACGTGGG TCTGACTTGC 
ACGGCTTTCC AGAGGAGGGA GGTAGGAGTG TCGGGTGCAC AGCCAAGCAG CGTCGGCCAG 
GATGCCCCAG TGGAGCCCGA ACCCCTAGCT GATCAGCAGC TTAGGTTCCG CTGCACCACT 
GGGAGGCCCA ACATTTCTCT GTCATCCTTC CACATCAAGA ATAGCGTGGC CCTGGCCTCC 
ATCCAGCTGC CCCCCAGCCT GTTCTCAACC CTTCCGGCTG CCCTGGCTCC CCCAGTCCCT 
CCAGATTGCA CCCTGCAACT GCTGGTCTTC AGAAATGGCC GTCTTTTCCG CAGCCACGGC 
AACAACACTT CCCGTCCTGG AGCAGCTGGG CCTGGCAAGA GGCGTGGTGT GGCCACCCCA 
GTCATATTTG CAGGAACCAG TGGCTGTGGT GTGGGAAACT TGACGGAGCC CGTGGCTGTG 
TCACTGAGGC ACTGGGCTGA AGGAGCTGAC CCCATGGCAG CTTGGTGGAA CCAGGACGGG 
CCTGGAGGCT GGAGTTCTGA AGGCTGCAGG CTCCGCTACA GCCAGCCCAA CGTCAGCTCC 
CTGTACTGCC AGCACTTGGG CAATGTGGCC GTGCTTATGG AGCTGAATGC ATTCCCGCGG 
GAGGCAGGAG GCTCTGGGGC TGGGTTGCAT CCCGTCGTGT ACCCCTGCAC GGCTTTGCTG 
CTACTCTGTC TCTTCTCCAC CATCATCACG TACATCCTCA ACCACAGCTC CATCCACGTG 
TCCCGGAAGG GTTGGCACAT GCTGCTGAAC CTTTGTTTCC ATATGGCCAT GACCTCTGCT 
GTCTTCGTGG GGGGCGTCAC GCTCACCAAC TACCAAATGG TTTGTCAAGC GGTGGGCATC 
ACTCTGCACT ACTCTTCCTT GTCGTCACTG CTCTGGATGG GGGTCAAGGC TCGAGTCCTC 
CACAAGGAGC TTAGTTGGAG GGCACCCCCT CTGGAAGAAG GGGAAGCAGC TCCGCCTGGT 
CCTCGCCCCA TGCTCCGTTG TTGGCTGGTG TGGCGTCCAA GCCTTGGTGC CTTCTACATA 
CCGGTGGCGT TGATTCTGCC TATCACCTGG ATCTACTTCT TGTGTGCAGG CCTTCACTTA 
CGGAGCCATG TGGCCCAGAA TCCAAAGCAG GGTAACAGGA TCTCTCTGGA GCCAGGGGAA 
GAGCTGAGGG GTTCCACCAG GCTCAGGAGT AGTGGCGTCC TCCTGAATGA CTCTGGTTCT 
CTTTTGGCTA CAGTTAGCGC AGGAGTAGGG ACACCTGCGC CCCCAGAGGA TGGTGATGGC 
GTATATTCTC CTGGAGTCCA GCTGGGGGCG CTGATGACCA CGCATTTCCT GTACCTGGCT 
ATGTGGGCTT GTGGTGCCTT GGCGGTGTCT CAGCGCTGGC TGCCCCGAGT GGTGTGTAGC 
TGTCTGTACG GCGTGGCAGC TTCAGCTCTT GGTCTGTTTG TCTTCACTCA CCACTGTGCC 
AGACGTAGAG ATGTCCGGGC TTCCTGGCGC GCCTGCTGCC CTCCTGCTTC GCCCTCGGCC 
TCCCATGTCC CAGCCCGGGC CCTGCCGACT GCTACAGAGG ATGGATCCCC AGTGTTGGGG 
GAGGGACCAG CCTCTCTCAA GTCCTCCCCA AGTGGCAGCA GTGGCCGCGC GCCGCCACCC 
CCCTGCAAAC TCACCAATCT GCAGGTGGCC CAGAGTCAGG TGTGCGAGGC AAGCGTGGCC 
GCCCGCGGAG ATGGAGAGCC AGAGCCCACC GGCTCCCGTG GCAGCCTAGC TCCCCGGCAC 
CATAACAACC TGCATCATGG GCGCCGAGTA CACAAGAGTC GGGCCAAGGG GCACCGAGCC 
GGAGAGACTG GTGGTAAGAG CCGGCTCAAG GCGTTGCGCG CGGGCACGTC CCCAGGAGCT 
CCCGAGCTGT TGTCCAGTGA GAGCGGCAGT TTGCACAACA GCCCGTCTGA CAGCTACCCG 
GGCAGCAGCC GCAACAGTCC AGGCGACGGT CTTCCACTCG AGGGTGAGCC CATGCTTACG 
CCGTCGGAGG GCAGTGACAC AAGCGCCGCG CCAATCGCTG AGACGGGGCG CCCCGGGCAG 
CGCCGCAGCG CCAGCCGTGA CAACCTCAAG GGCAGCGGCA GCGCGCTGGA GAGGGAGAGC 
AAGCGCCGCT CCTATCCGCT CAACACTACC AGTCTTAACG GTGCCCCCAA AGGGGGCAAG 
TATGAGGATG CCAGTGTAAC TGGTGCAGAG GCCATAGCGG GAGGCTCCAT GAAGACTGGC 
CTCTGGAAAA GCGAGACCAC CGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509285.3
CDS	328..4374
Translation	MAPRPALGRPGLPLSTPPARPGPSGRRAVRPLRCRSPRGDGLMGAGGRRMPVPPARLLLLPLLPCLLLLAPGTRGAPGCPVPIRGCKCSGERPKGLSGGAHNPARRRVVCGGGDLPEPPDPGLLPNGTITLLLSNNKITGLRNGSFLGLSLLEKLDLRSNVISTVQPGAFLGLGELKRLDLSNNRIGCLTSETFQGLPRLLRLNISGNIYSSLQPGVFDELPALKIVDFGTEFLTCDCRLRWLLPWARNHSLQLSERTLCAYPSALHAHALSSLQESQLRCEGALELHTHYLIPSLRQVVFQGDRLPFQCSASYLGNDTRIHWYHNGAPMESDEQAGIVLAENLIHDCTFITSELTLSHIGVWASGEWECSVSTVQGNTSKKVEIVVLETSASYCPAERVTNNRGDFRWPRTLAGITAYQSCLQYPFTSVPLSGGAPGTRASRRCDRAGRWEPGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDMASNLMLVDEHLLWLAQREDKACSGIVGALERIGGAALSPHAQHISVNSRNVALEAYLIKPHSYVGLTCTAFQRREVGVSGAQPSSVGQDAPVEPEPLADQQLRFRCTTGRPNISLSSFHIKNSVALASIQLPPSLFSTLPAALAPPVPPDCTLQLLVFRNGRLFRSHGNNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSLRHWAEGADPMAAWWNQDGPGGWSSEGCRLRYSQPNVSSLYCQHLGNVAVLMELNAFPREAGGSGAGLHPVVYPCTALLLLCLFSTIITYILNHSSIHVSRKGWHMLLNLCFHMAMTSAVFVGGVTLTNYQMVCQAVGITLHYSSLSSLLWMGVKARVLHKELSWRAPPLEEGEAAPPGPRPMLRCWLVWRPSLGAFYIPVALILPITWIYFLCAGLHLRSHVAQNPKQGNRISLEPGEELRGSTRLRSSGVLLNDSGSLLATVSAGVGTPAPPEDGDGVYSPGVQLGALMTTHFLYLAMWACGALAVSQRWLPRVVCSCLYGVAASALGLFVFTHHCARRRDVRASWRACCPPASPSASHVPARALPTATEDGSPVLGEGPASLKSSPSGSSGRAPPPPCKLTNLQVAQSQVCEASVAARGDGEPEPTGSRGSLAPRHHNNLHHGRRVHKSRAKGHRAGETGGKSRLKALRAGTSPGAPELLSSESGSLHNSPSDSYPGSSRNSPGDGLPLEGEPMLTPSEGSDTSAAPIAETGRPGQRRSASRDNLKGSGSALERESKRRSYPLNTTSLNGAPKGGKYEDASVTGAEAIAGGSMKTGLWKSETTV

Target ORF information:

RefSeq Version	XM_006509222.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509222.3

ORF Insert Sequence:

ATGGCCCCCC GCCCCGCCCT CGGGCGCCCG GGACTCCCGC TGAGCACTCC TCCCGCACGC 
CCGGGTCCCT CCGGCCGGCG AGCCGTCCGG CCCCTGCGCT GTAGGTCCCC GCGGGGCGAT 
GGGTTGATGG GCGCGGGGGG ACGCAGGATG CCGGTGCCTC CCGCGCGTTT GCTGCTGCTG 
CCGCTGCTGC CTTGTCTTCT GCTCCTGGCT CCTGGAACTC GGGGTGCGCC TGGCTGCCCG 
GTCCCTATCC GCGGTTGCAA GTGCTCTGGG GAGCGGCCCA AGGGACTAAG TGGCGGCGCC 
CACAACCCGG CTCGAAGGAG GGTGGTGTGC GGCGGTGGGG ATCTCCCCGA ACCTCCAGAT 
CCCGGCCTTC TGCCAAACGG CACCATCACC TTGCTCTTGA GCAACAACAA GATTACTGGG 
CTCCGCAATG GATCCTTCTT GGGACTGTCC CTGTTGGAGA AGTTGGACCT GAGGAGCAAT 
GTCATCAGCA CTGTGCAGCC TGGAGCCTTC CTAGGTCTGG GAGAGCTAAA ACGCTTAGAT 
CTCTCCAACA ATCGGATTGG CTGTCTCACC TCTGAGACAT TTCAAGGGCT CCCTAGACTT 
CTCAGACTAA ACATATCTGG AAACATCTAC TCTAGTCTGC AACCTGGGGT CTTTGATGAG 
CTGCCAGCCC TTAAGATTGT GGACTTTGGT ACTGAGTTTC TGACCTGTGA CTGCCGCCTG 
CGCTGGCTGC TGCCCTGGGC CCGGAATCAC TCCCTGCAGC TGTCTGAGCG CACACTCTGT 
GCCTACCCCA GTGCCCTGCA CGCCCATGCC CTGAGCAGCC TCCAGGAGTC CCAGCTTCGC 
TGTGAAGGGG CCCTGGAACT GCACACCCAC TACCTCATCC CATCCCTTCG CCAAGTGGTG 
TTCCAGGGTG ACCGCCTGCC CTTCCAGTGC TCAGCCAGCT ACTTGGGCAA CGATACCCGG 
ATCCACTGGT ACCACAATGG GGCTCCTATG GAGAGCGATG AGCAGGCCGG CATCGTCCTT 
GCTGAAAACC TTATCCATGA TTGCACCTTC ATCACCAGTG AGCTGACCCT GTCTCACATT 
GGTGTGTGGG CCTCTGGTGA ATGGGAGTGC TCCGTGTCCA CAGTCCAAGG CAACACCAGC 
AAGAAGGTGG AGATAGTAGT GCTGGAGACC TCTGCCTCCT ACTGCCCTGC AGAGCGGGTG 
ACCAACAACC GTGGGGACTT CAGGTGGCCC CGAACCTTGG CTGGCATCAC GGCTTACCAG 
TCCTGTTTAC AGTACCCCTT CACCTCTGTG CCCTTGAGTG GGGGAGCCCC GGGTACCCGA 
GCCTCACGCA GGTGTGACCG AGCTGGCCGC TGGGAGCCAG GGGACTACTC GCACTGTCTG 
TACACCAATG ACATCACTCG GGTGCTCTAC ACCTTTGTGC TGATGCCCAT CAACGCCTCC 
AATGCATTGA CGTTGGCCCA CCAGCTGCGA GTGTATACCG CAGAGGCCGC CAGCTTCTCA 
GACATGATGG ACGTGGTCTA TGTGGCTCAG ATGATCCAGA AGTTTTTGGG TTACGTTGAC 
CAGATCAAGG AGCTGGTGGA GGTGATGGTG GACATGGCCA GCAACCTGAT GCTGGTGGAT 
GAGCACCTTC TGTGGCTGGC CCAGAGAGAA GACAAAGCCT GCAGTGGCAT TGTGGGTGCC 
CTGGAGCGAA TCGGAGGAGC TGCTCTTAGC CCCCATGCCC AGCACATCTC TGTGAATTCA 
AGAAATGTGG CACTGGAGGC CTACCTCATC AAGCCTCACA GCTACGTGGG TCTGACTTGC 
ACGGCTTTCC AGAGGAGGGA GGTAGGAGTG TCGGGTGCAC AGCCAAGCAG CGTCGGCCAG 
GATGCCCCAG TGGAGCCCGA ACCCCTAGCT GATCAGCAGC TTAGGTTCCG CTGCACCACT 
GGGAGGCCCA ACATTTCTCT GTCATCCTTC CACATCAAGA ATAGCGTGGC CCTGGCCTCC 
ATCCAGCTGC CCCCCAGCCT GTTCTCAACC CTTCCGGCTG CCCTGGCTCC CCCAGTCCCT 
CCAGATTGCA CCCTGCAACT GCTGGTCTTC AGAAATGGCC GTCTTTTCCG CAGCCACGGC 
AACAACACTT CCCGTCCTGG AGCAGCTGGG CCTGGCAAGA GGCGTGGTGT GGCCACCCCA 
GTCATATTTG CAGGAACCAG TGGCTGTGGT GTGGGAAACT TGACGGAGCC CGTGGCTGTG 
TCACTGAGGC ACTGGGCTGA AGGAGCTGAC CCCATGGCAG CTTGGTGGAA CCAGGACGGG 
CCTGGAGGCT GGAGTTCTGA AGGCTGCAGG CTCCGCTACA GCCAGCCCAA CGTCAGCTCC 
CTGTACTGCC AGCACTTGGG CAATGTGGCC GTGCTTATGG AGCTGAATGC ATTCCCGCGG 
GAGGCAGGAG GCTCTGGGGC TGGGTTGCAT CCCGTCGTGT ACCCCTGCAC GGCTTTGCTG 
CTACTCTGTC TCTTCTCCAC CATCATCACG TACATCCTCA ACCACAGCTC CATCCACGTG 
TCCCGGAAGG GTTGGCACAT GCTGCTGAAC CTTTGTTTCC ATATGGCCAT GACCTCTGCT 
GTCTTCGTGG GGGGCGTCAC GCTCACCAAC TACCAAATGG TTTGTCAAGC GGTGGGCATC 
ACTCTGCACT ACTCTTCCTT GTCGTCACTG CTCTGGATGG GGGTCAAGGC TCGAGTCCTC 
CACAAGGAGC TTAGTTGGAG GGCACCCCCT CTGGAAGAAG GGGAAGCAGC TCCGCCTGGT 
CCTCGCCCCA TGCTCCGTTG TTGGCTGGTG TGGCGTCCAA GCCTTGGTGC CTTCTACATA 
CCGGTGGCGT TGATTCTGCC TATCACCTGG ATCTACTTCT TGTGTGCAGG CCTTCACTTA 
CGGAGCCATG TGGCCCAGAA TCCAAAGCAG GGTAACAGGA TCTCTCTGGA GCCAGGGGAA 
GAGCTGAGGG GTTCCACCAG GCTCAGGAGT AGTGGCGTCC TCCTGAATGA CTCTGGTTCT 
CTTTTGGCTA CAGTTAGCGC AGGAGTAGGG ACACCTGCGC CCCCAGAGGA TGGTGATGGC 
GTATATTCTC CTGGAGTCCA GCTGGGGGCG CTGATGACCA CGCATTTCCT GTACCTGGCT 
ATGTGGGCTT GTGGTGCCTT GGCGGTGTCT CAGCGCTGGC TGCCCCGAGT GGTGTGTAGC 
TGTCTGTACG GCGTGGCAGC TTCAGCTCTT GGTCTGTTTG TCTTCACTCA CCACTGTGCC 
AGACGTAGAG ATGTCCGGGC TTCCTGGCGC GCCTGCTGCC CTCCTGCTTC GCCCTCGGCC 
TCCCATGTCC CAGCCCGGGC CCTGCCGACT GCTACAGAGG ATGGATCCCC AGTGTTGGGG 
GAGGGACCAG CCTCTCTCAA GTCCTCCCCA AGTGGCAGCA GTGGCCGCGC GCCGCCACCC 
CCCTGCAAAC TCACCAATCT GCAGGTGGCC CAGAGTCAGG TGTGCGAGGC AAGCGTGGCC 
GCCCGCGGAG ATGGAGAGCC AGAGCCCACC GGCTCCCGTG GCAGCCTAGC TCCCCGGCAC 
CATAACAACC TGCATCATGG GCGCCGAGTA CACAAGAGTC GGGCCAAGGG GCACCGAGCC 
GGAGAGACTG GTGGTAAGAG CCGGCTCAAG GCGTTGCGCG CGGGCACGTC CCCAGGAGCT 
CCCGAGCTGT TGTCCAGTGA GAGCGGCAGT TTGCACAACA GCCCGTCTGA CAGCTACCCG 
GGCAGCAGCC GCAACAGTCC AGGCGACGGT CTTCCACTCG AGGGTGAGCC CATGCTTACG 
CCGTCGGAGG GCAGTGACAC AAGCGCCGCG CCAATCGCTG AGACGGGGCG CCCCGGGCAG 
CGCCGCAGCG CCAGCCGTGA CAACCTCAAG GGCAGCGGCA GCGCGCTGGA GAGGGAGAGC 
AAGCGCCGCT CCTATCCGCT CAACACTACC AGTCTTAACG GTGCCCCCAA AGGGGGCAAG 
TATGAGGATG CCAGTGTAAC TGGTGCAGAG GCCATAGCGG GAGGCTCCAT GAAGACTGGC 
CTCTGGAAAA GCGAGACCAC CGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgra2 ( XM_006509223.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509286.3 Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X2, mRNA.

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CloneID	OMu82607
Clone ID Related Accession (Same CDS sequence)	XM_006509223.4 , XM_006509223.3
Accession Version	XM_006509223.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor A2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509223.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCC GCCCCGCCCT CGGGCGCCCG GGACTCCCGC TGAGCACTCC TCCCGCACGC 
CCGGGTCCCT CCGGCCGGCG AGCCGTCCGG CCCCTGCGCT GTAGGTCCCC GCGGGGCGAT 
GGGTTGATGG GCGCGGGGGG ACGCAGGATG CCGGTGCCTC CCGCGCGTTT GCTGCTGCTG 
CCGCTGCTGC CTTGTCTTCT GCTCCTGGCT CCTGGAACTC GGGGTGCGCC TGGCTGCCCG 
GTCCCTATCC GCGGTTGCAA GTGCTCTGGG GAGCGGCCCA AGGGACTAAG TGGCGGCGCC 
CACAACCCGG CTCGAAGGAG GGTGGTGTGC GGCGGTGGGG ATCTCCCCGA ACCTCCAGAT 
CCCGGCCTTC TGCCAAACGG CACCATCACC TTGCTCTTGA GCAACAACAA GATTACTGGG 
CTCCGCAATG GATCCTTCTT GGGACTGTCC CTGTTGGAGA AGTTGGACCT GAGGAGCAAT 
GTCATCAGCA CTGTGCAGCC TGGAGCCTTC CTAGGTCTGG GAGAGCTAAA ACGCTTAGAT 
CTCTCCAACA ATCGGATTGG CTGTCTCACC TCTGAGACAT TTCAAGGGCT CCCTAGACTT 
CTCAGACTAA ACATATCTGG AAACATCTAC TCTAGTCTGC AACCTGGGGT CTTTGATGAG 
CTGCCAGCCC TTAAGATTGT GGACTTTGGT ACTGAGTTTC TGACCTGTGA CTGCCGCCTG 
CGCTGGCTGC TGCCCTGGGC CCGGAATCAC TCCCTGCAGC TGTCTGAGCG CACACTCTGT 
GCCTACCCCA GTGCCCTGCA CGCCCATGCC CTGAGCAGCC TCCAGGAGTC CCAGCTTCGC 
TGTGAAGGGG CCCTGGAACT GCACACCCAC TACCTCATCC CATCCCTTCG CCAAGTGGTG 
TTCCAGGGTG ACCGCCTGCC CTTCCAGTGC TCAGCCAGCT ACTTGGGCAA CGATACCCGG 
ATCCACTGGT ACCACAATGG GGCTCCTATG GAGAGCGATG AGCAGGCCGG CATCGTCCTT 
GCTGAAAACC TTATCCATGA TTGCACCTTC ATCACCAGTG AGCTGACCCT GTCTCACATT 
GGTGTGTGGG CCTCTGGTGA ATGGGAGTGC TCCGTGTCCA CAGTCCAAGG CAACACCAGC 
AAGAAGGTGG AGATAGTAGT GCTGGAGACC TCTGCCTCCT ACTGCCCTGC AGAGCGGGTG 
ACCAACAACC GTGGGGACTT CAGGTGGCCC CGAACCTTGG CTGGCATCAC GGCTTACCAG 
TCCTGTTTAC AGTACCCCTT CACCTCTGTG CCCTTGAGTG GGGGAGCCCC GGGTACCCGA 
GCCTCACGCA GGTGTGACCG AGCTGGCCGC TGGGAGCCAG GGGACTACTC GCACTGTCTG 
TACACCAATG ACATCACTCG GGTGCTCTAC ACCTTTGTGC TGATGCCCAT CAACGCCTCC 
AATGCATTGA CGTTGGCCCA CCAGCTGCGA GTGTATACCG CAGAGGCCGC CAGCTTCTCA 
GACATGATGG ACGTGGTCTA TGTGGCTCAG ATGATCCAGA AGTTTTTGGG TTACGTTGAC 
CAGATCAAGG AGCTGGTGGA GGTGATGGTG GACATGGCCA GCAACCTGAT GCTGGTGGAT 
GAGCACCTTC TGTGGCTGGC CCAGAGAGAA GACAAAGCCT GCAGTGGCAT TGTGGGTGCC 
CTGGAGCGAA TCGGAGGAGC TGCTCTTAGC CCCCATGCCC AGCACATCTC TGTGAATTCA 
AGAAATGTGG CACTGGAGGC CTACCTCATC AAGCCTCACA GCTACGTGGG TCTGACTTGC 
ACGGCTTTCC AGAGGAGGGA GGTAGGAGTG TCGGGTGCAC AGCCAAGCAG CGTCGGCCAG 
GATGCCCCAG TGGAGCCCGA ACCCCTAGCT GATCAGCAGC TTAGGTTCCG CTGCACCACT 
GGGAGGCCCA ACATTTCTCT GTCATCCTTC CACATCAAGA ATAGCGTGGC CCTGGCCTCC 
ATCCAGCTGC CCCCCAGCCT GTTCTCAACC CTTCCGGCTG CCCTGGCTCC CCCAGTCCCT 
CCAGATTGCA CCCTGCAACT GCTGGTCTTC AGAAATGGCC GTCTTTTCCG CAGCCACGGC 
AACAACACTT CCCGTCCTGG AGCAGCTGGG CCTGGCAAGA GGCGTGGTGT GGCCACCCCA 
GTCATATTTG CAGGAACCAG TGGCTGTGGT GTGGGAAACT TGACGGAGCC CGTGGCTGTG 
TCACTGAGGC ACTGGGCTGA AGGAGCTGAC CCCATGGCAG CTTGGTGGAA CCAGGACGGG 
CCTGGAGGCT GGAGTTCTGA AGGCTGCAGG CTCCGCTACA GCCAGCCCAA CGTCAGCTCC 
CTGTACTGCC AGCACTTGGG CAATGTGGCC GTGCTTATGG AGCTGAATGC ATTCCCGCGG 
GAGGCAGGAG GCTCTGGGGC TGGGTTGCAT CCCGTCGTGT ACCCCTGCAC GGCTTTGCTG 
CTACTCTGTC TCTTCTCCAC CATCATCACG TACATCCTCA ACCACAGCTC CATCCACGTG 
TCCCGGAAGG GTTGGCACAT GCTGCTGAAC CTTTGTTTCC ATATGGCCAT GACCTCTGCT 
GTCTTCGTGG GGGGCGTCAC GCTCACCAAC TACCAAATGG TTTGTCAAGC GGTGGGCATC 
ACTCTGCACT ACTCTTCCTT GTCGTCACTG CTCTGGATGG GGGTCAAGGC TCGAGTCCTC 
CACAAGGAGC TTAGTTGGAG GGCACCCCCT CTGGAAGAAG GGGAAGCAGC TCCGCCTGGT 
CCTCGCCCCA TGCTCCGGTT CTACTTGATT GCTGGAGGGA TCCCCCTCAT AATCTGCGGC 
ATCACCGCTG CGGTCAACAT CCACAACTAC CGGGACCACA GTCCCTATTG TTGGCTGGTG 
TGGCGTCCAA GCCTTGGTGC CTTCTACATA CCGGTGGCGT TGATTCTGCC TATCACCTGG 
ATCTACTTCT TGTGTGCAGG CCTTCACTTA CGGAGCCATG TGGCCCAGAA TCCAAAGCAG 
GTTAGCGCAG GAGTAGGGAC ACCTGCGCCC CCAGAGGATG GTGATGGCGT ATATTCTCCT 
GGAGTCCAGC TGGGGGCGCT GATGACCACG CATTTCCTGT ACCTGGCTAT GTGGGCTTGT 
GGTGCCTTGG CGGTGTCTCA GCGCTGGCTG CCCCGAGTGG TGTGTAGCTG TCTGTACGGC 
GTGGCAGCTT CAGCTCTTGG TCTGTTTGTC TTCACTCACC ACTGTGCCAG ACGTAGAGAT 
GTCCGGGCTT CCTGGCGCGC CTGCTGCCCT CCTGCTTCGC CCTCGGCCTC CCATGTCCCA 
GCCCGGGCCC TGCCGACTGC TACAGAGGAT GGATCCCCAG TGTTGGGGGA GGGACCAGCC 
TCTCTCAAGT CCTCCCCAAG TGGCAGCAGT GGCCGCGCGC CGCCACCCCC CTGCAAACTC 
ACCAATCTGC AGGTGGCCCA GAGTCAGGTG TGCGAGGCAA GCGTGGCCGC CCGCGGAGAT 
GGAGAGCCAG AGCCCACCGG CTCCCGTGGC AGCCTAGCTC CCCGGCACCA TAACAACCTG 
CATCATGGGC GCCGAGTACA CAAGAGTCGG GCCAAGGGGC ACCGAGCCGG AGAGACTGGT 
GGTAAGAGCC GGCTCAAGGC GTTGCGCGCG GGCACGTCCC CAGGAGCTCC CGAGCTGTTG 
TCCAGTGAGA GCGGCAGTTT GCACAACAGC CCGTCTGACA GCTACCCGGG CAGCAGCCGC 
AACAGTCCAG GCGACGGTCT TCCACTCGAG GGTGAGCCCA TGCTTACGCC GTCGGAGGGC 
AGTGACACAA GCGCCGCGCC AATCGCTGAG ACGGGGCGCC CCGGGCAGCG CCGCAGCGCC 
AGCCGTGACA ACCTCAAGGG CAGCGGCAGC GCGCTGGAGA GGGAGAGCAA GCGCCGCTCC 
TATCCGCTCA ACACTACCAG TCTTAACGGT GCCCCCAAAG GGGGCAAGTA TGAGGATGCC 
AGTGTAACTG GTGCAGAGGC CATAGCGGGA GGCTCCATGA AGACTGGCCT CTGGAAAAGC 
GAGACCACCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509286.3
CDS	328..4362
Translation	MAPRPALGRPGLPLSTPPARPGPSGRRAVRPLRCRSPRGDGLMGAGGRRMPVPPARLLLLPLLPCLLLLAPGTRGAPGCPVPIRGCKCSGERPKGLSGGAHNPARRRVVCGGGDLPEPPDPGLLPNGTITLLLSNNKITGLRNGSFLGLSLLEKLDLRSNVISTVQPGAFLGLGELKRLDLSNNRIGCLTSETFQGLPRLLRLNISGNIYSSLQPGVFDELPALKIVDFGTEFLTCDCRLRWLLPWARNHSLQLSERTLCAYPSALHAHALSSLQESQLRCEGALELHTHYLIPSLRQVVFQGDRLPFQCSASYLGNDTRIHWYHNGAPMESDEQAGIVLAENLIHDCTFITSELTLSHIGVWASGEWECSVSTVQGNTSKKVEIVVLETSASYCPAERVTNNRGDFRWPRTLAGITAYQSCLQYPFTSVPLSGGAPGTRASRRCDRAGRWEPGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDMASNLMLVDEHLLWLAQREDKACSGIVGALERIGGAALSPHAQHISVNSRNVALEAYLIKPHSYVGLTCTAFQRREVGVSGAQPSSVGQDAPVEPEPLADQQLRFRCTTGRPNISLSSFHIKNSVALASIQLPPSLFSTLPAALAPPVPPDCTLQLLVFRNGRLFRSHGNNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSLRHWAEGADPMAAWWNQDGPGGWSSEGCRLRYSQPNVSSLYCQHLGNVAVLMELNAFPREAGGSGAGLHPVVYPCTALLLLCLFSTIITYILNHSSIHVSRKGWHMLLNLCFHMAMTSAVFVGGVTLTNYQMVCQAVGITLHYSSLSSLLWMGVKARVLHKELSWRAPPLEEGEAAPPGPRPMLRFYLIAGGIPLIICGITAAVNIHNYRDHSPYCWLVWRPSLGAFYIPVALILPITWIYFLCAGLHLRSHVAQNPKQVSAGVGTPAPPEDGDGVYSPGVQLGALMTTHFLYLAMWACGALAVSQRWLPRVVCSCLYGVAASALGLFVFTHHCARRRDVRASWRACCPPASPSASHVPARALPTATEDGSPVLGEGPASLKSSPSGSSGRAPPPPCKLTNLQVAQSQVCEASVAARGDGEPEPTGSRGSLAPRHHNNLHHGRRVHKSRAKGHRAGETGGKSRLKALRAGTSPGAPELLSSESGSLHNSPSDSYPGSSRNSPGDGLPLEGEPMLTPSEGSDTSAAPIAETGRPGQRRSASRDNLKGSGSALERESKRRSYPLNTTSLNGAPKGGKYEDASVTGAEAIAGGSMKTGLWKSETTV

Target ORF information:

RefSeq Version	XM_006509223.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509223.3

ORF Insert Sequence:

ATGGCCCCCC GCCCCGCCCT CGGGCGCCCG GGACTCCCGC TGAGCACTCC TCCCGCACGC 
CCGGGTCCCT CCGGCCGGCG AGCCGTCCGG CCCCTGCGCT GTAGGTCCCC GCGGGGCGAT 
GGGTTGATGG GCGCGGGGGG ACGCAGGATG CCGGTGCCTC CCGCGCGTTT GCTGCTGCTG 
CCGCTGCTGC CTTGTCTTCT GCTCCTGGCT CCTGGAACTC GGGGTGCGCC TGGCTGCCCG 
GTCCCTATCC GCGGTTGCAA GTGCTCTGGG GAGCGGCCCA AGGGACTAAG TGGCGGCGCC 
CACAACCCGG CTCGAAGGAG GGTGGTGTGC GGCGGTGGGG ATCTCCCCGA ACCTCCAGAT 
CCCGGCCTTC TGCCAAACGG CACCATCACC TTGCTCTTGA GCAACAACAA GATTACTGGG 
CTCCGCAATG GATCCTTCTT GGGACTGTCC CTGTTGGAGA AGTTGGACCT GAGGAGCAAT 
GTCATCAGCA CTGTGCAGCC TGGAGCCTTC CTAGGTCTGG GAGAGCTAAA ACGCTTAGAT 
CTCTCCAACA ATCGGATTGG CTGTCTCACC TCTGAGACAT TTCAAGGGCT CCCTAGACTT 
CTCAGACTAA ACATATCTGG AAACATCTAC TCTAGTCTGC AACCTGGGGT CTTTGATGAG 
CTGCCAGCCC TTAAGATTGT GGACTTTGGT ACTGAGTTTC TGACCTGTGA CTGCCGCCTG 
CGCTGGCTGC TGCCCTGGGC CCGGAATCAC TCCCTGCAGC TGTCTGAGCG CACACTCTGT 
GCCTACCCCA GTGCCCTGCA CGCCCATGCC CTGAGCAGCC TCCAGGAGTC CCAGCTTCGC 
TGTGAAGGGG CCCTGGAACT GCACACCCAC TACCTCATCC CATCCCTTCG CCAAGTGGTG 
TTCCAGGGTG ACCGCCTGCC CTTCCAGTGC TCAGCCAGCT ACTTGGGCAA CGATACCCGG 
ATCCACTGGT ACCACAATGG GGCTCCTATG GAGAGCGATG AGCAGGCCGG CATCGTCCTT 
GCTGAAAACC TTATCCATGA TTGCACCTTC ATCACCAGTG AGCTGACCCT GTCTCACATT 
GGTGTGTGGG CCTCTGGTGA ATGGGAGTGC TCCGTGTCCA CAGTCCAAGG CAACACCAGC 
AAGAAGGTGG AGATAGTAGT GCTGGAGACC TCTGCCTCCT ACTGCCCTGC AGAGCGGGTG 
ACCAACAACC GTGGGGACTT CAGGTGGCCC CGAACCTTGG CTGGCATCAC GGCTTACCAG 
TCCTGTTTAC AGTACCCCTT CACCTCTGTG CCCTTGAGTG GGGGAGCCCC GGGTACCCGA 
GCCTCACGCA GGTGTGACCG AGCTGGCCGC TGGGAGCCAG GGGACTACTC GCACTGTCTG 
TACACCAATG ACATCACTCG GGTGCTCTAC ACCTTTGTGC TGATGCCCAT CAACGCCTCC 
AATGCATTGA CGTTGGCCCA CCAGCTGCGA GTGTATACCG CAGAGGCCGC CAGCTTCTCA 
GACATGATGG ACGTGGTCTA TGTGGCTCAG ATGATCCAGA AGTTTTTGGG TTACGTTGAC 
CAGATCAAGG AGCTGGTGGA GGTGATGGTG GACATGGCCA GCAACCTGAT GCTGGTGGAT 
GAGCACCTTC TGTGGCTGGC CCAGAGAGAA GACAAAGCCT GCAGTGGCAT TGTGGGTGCC 
CTGGAGCGAA TCGGAGGAGC TGCTCTTAGC CCCCATGCCC AGCACATCTC TGTGAATTCA 
AGAAATGTGG CACTGGAGGC CTACCTCATC AAGCCTCACA GCTACGTGGG TCTGACTTGC 
ACGGCTTTCC AGAGGAGGGA GGTAGGAGTG TCGGGTGCAC AGCCAAGCAG CGTCGGCCAG 
GATGCCCCAG TGGAGCCCGA ACCCCTAGCT GATCAGCAGC TTAGGTTCCG CTGCACCACT 
GGGAGGCCCA ACATTTCTCT GTCATCCTTC CACATCAAGA ATAGCGTGGC CCTGGCCTCC 
ATCCAGCTGC CCCCCAGCCT GTTCTCAACC CTTCCGGCTG CCCTGGCTCC CCCAGTCCCT 
CCAGATTGCA CCCTGCAACT GCTGGTCTTC AGAAATGGCC GTCTTTTCCG CAGCCACGGC 
AACAACACTT CCCGTCCTGG AGCAGCTGGG CCTGGCAAGA GGCGTGGTGT GGCCACCCCA 
GTCATATTTG CAGGAACCAG TGGCTGTGGT GTGGGAAACT TGACGGAGCC CGTGGCTGTG 
TCACTGAGGC ACTGGGCTGA AGGAGCTGAC CCCATGGCAG CTTGGTGGAA CCAGGACGGG 
CCTGGAGGCT GGAGTTCTGA AGGCTGCAGG CTCCGCTACA GCCAGCCCAA CGTCAGCTCC 
CTGTACTGCC AGCACTTGGG CAATGTGGCC GTGCTTATGG AGCTGAATGC ATTCCCGCGG 
GAGGCAGGAG GCTCTGGGGC TGGGTTGCAT CCCGTCGTGT ACCCCTGCAC GGCTTTGCTG 
CTACTCTGTC TCTTCTCCAC CATCATCACG TACATCCTCA ACCACAGCTC CATCCACGTG 
TCCCGGAAGG GTTGGCACAT GCTGCTGAAC CTTTGTTTCC ATATGGCCAT GACCTCTGCT 
GTCTTCGTGG GGGGCGTCAC GCTCACCAAC TACCAAATGG TTTGTCAAGC GGTGGGCATC 
ACTCTGCACT ACTCTTCCTT GTCGTCACTG CTCTGGATGG GGGTCAAGGC TCGAGTCCTC 
CACAAGGAGC TTAGTTGGAG GGCACCCCCT CTGGAAGAAG GGGAAGCAGC TCCGCCTGGT 
CCTCGCCCCA TGCTCCGGTT CTACTTGATT GCTGGAGGGA TCCCCCTCAT AATCTGCGGC 
ATCACCGCTG CGGTCAACAT CCACAACTAC CGGGACCACA GTCCCTATTG TTGGCTGGTG 
TGGCGTCCAA GCCTTGGTGC CTTCTACATA CCGGTGGCGT TGATTCTGCC TATCACCTGG 
ATCTACTTCT TGTGTGCAGG CCTTCACTTA CGGAGCCATG TGGCCCAGAA TCCAAAGCAG 
GTTAGCGCAG GAGTAGGGAC ACCTGCGCCC CCAGAGGATG GTGATGGCGT ATATTCTCCT 
GGAGTCCAGC TGGGGGCGCT GATGACCACG CATTTCCTGT ACCTGGCTAT GTGGGCTTGT 
GGTGCCTTGG CGGTGTCTCA GCGCTGGCTG CCCCGAGTGG TGTGTAGCTG TCTGTACGGC 
GTGGCAGCTT CAGCTCTTGG TCTGTTTGTC TTCACTCACC ACTGTGCCAG ACGTAGAGAT 
GTCCGGGCTT CCTGGCGCGC CTGCTGCCCT CCTGCTTCGC CCTCGGCCTC CCATGTCCCA 
GCCCGGGCCC TGCCGACTGC TACAGAGGAT GGATCCCCAG TGTTGGGGGA GGGACCAGCC 
TCTCTCAAGT CCTCCCCAAG TGGCAGCAGT GGCCGCGCGC CGCCACCCCC CTGCAAACTC 
ACCAATCTGC AGGTGGCCCA GAGTCAGGTG TGCGAGGCAA GCGTGGCCGC CCGCGGAGAT 
GGAGAGCCAG AGCCCACCGG CTCCCGTGGC AGCCTAGCTC CCCGGCACCA TAACAACCTG 
CATCATGGGC GCCGAGTACA CAAGAGTCGG GCCAAGGGGC ACCGAGCCGG AGAGACTGGT 
GGTAAGAGCC GGCTCAAGGC GTTGCGCGCG GGCACGTCCC CAGGAGCTCC CGAGCTGTTG 
TCCAGTGAGA GCGGCAGTTT GCACAACAGC CCGTCTGACA GCTACCCGGG CAGCAGCCGC 
AACAGTCCAG GCGACGGTCT TCCACTCGAG GGTGAGCCCA TGCTTACGCC GTCGGAGGGC 
AGTGACACAA GCGCCGCGCC AATCGCTGAG ACGGGGCGCC CCGGGCAGCG CCGCAGCGCC 
AGCCGTGACA ACCTCAAGGG CAGCGGCAGC GCGCTGGAGA GGGAGAGCAA GCGCCGCTCC 
TATCCGCTCA ACACTACCAG TCTTAACGGT GCCCCCAAAG GGGGCAAGTA TGAGGATGCC 
AGTGTAACTG GTGCAGAGGC CATAGCGGGA GGCTCCATGA AGACTGGCCT CTGGAAAAGC 
GAGACCACCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgra2 ( XM_006509224.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509287.3 Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X3, mRNA.

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CloneID	OMu82608
Clone ID Related Accession (Same CDS sequence)	XM_006509224.3 , XM_006509224.4
Accession Version	XM_006509224.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor A2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509224.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCC GCCCCGCCCT CGGGCGCCCG GGACTCCCGC TGAGCACTCC TCCCGCACGC 
CCGGGTCCCT CCGGCCGGCG AGCCGTCCGG CCCCTGCGCT GTAGGTCCCC GCGGGGCGAT 
GGGTTGATGG GCGCGGGGGG ACGCAGGATG CCGGTGCCTC CCGCGCGTTT GCTGCTGCTG 
CCGCTGCTGC CTTGTCTTCT GCTCCTGGCT CCTGGAACTC GGGGTGCGCC TGGCTGCCCG 
GTCCCTATCC GCGGTTGCAA GTGCTCTGGG GAGCGGCCCA AGGGACTAAG TGGCGGCGCC 
CACAACCCGG CTCGAAGGAG GGTGGTGTGC GGCGGTGGGG ATCTCCCCGA ACCTCCAGAT 
CCCGGCCTTC TGCCAAACGG CACCATCACC TTGCTCTTGA GCAACAACAA GATTACTGGG 
CTCCGCAATG GATCCTTCTT GGGACTGTCC CTGTTGGAGA AGTTGGACCT GAGGAGCAAT 
GTCATCAGCA CTGTGCAGCC TGGAGCCTTC CTAGGTCTGG GAGAGCTAAA ACGCTTAGAT 
CTCTCCAACA ATCGGATTGG CTGTCTCACC TCTGAGACAT TTCAAGGGCT CCCTAGACTT 
CTCAGACTAA ACATATCTGG AAACATCTAC TCTAGTCTGC AACCTGGGGT CTTTGATGAG 
CTGCCAGCCC TTAAGATTGT GGACTTTGGT ACTGAGTTTC TGACCTGTGA CTGCCGCCTG 
CGCTGGCTGC TGCCCTGGGC CCGGAATCAC TCCCTGCAGC TGTCTGAGCG CACACTCTGT 
GCCTACCCCA GTGCCCTGCA CGCCCATGCC CTGAGCAGCC TCCAGGAGTC CCAGCTTCGC 
TGTGAAGGGG CCCTGGAACT GCACACCCAC TACCTCATCC CATCCCTTCG CCAAGTGGTG 
TTCCAGGGTG ACCGCCTGCC CTTCCAGTGC TCAGCCAGCT ACTTGGGCAA CGATACCCGG 
ATCCACTGGT ACCACAATGG GGCTCCTATG GAGAGCGATG AGCAGGCCGG CATCGTCCTT 
GCTGAAAACC TTATCCATGA TTGCACCTTC ATCACCAGTG AGCTGACCCT GTCTCACATT 
GGTGTGTGGG CCTCTGGTGA ATGGGAGTGC TCCGTGTCCA CAGTCCAAGG CAACACCAGC 
AAGAAGGTGG AGATAGTAGT GCTGGAGACC TCTGCCTCCT ACTGCCCTGC AGAGCGGGTG 
ACCAACAACC GTGGGGACTT CAGGTGGCCC CGAACCTTGG CTGGCATCAC GGCTTACCAG 
TCCTGTTTAC AGTACCCCTT CACCTCTGTG CCCTTGAGTG GGGGAGCCCC GGGTACCCGA 
GCCTCACGCA GGTGTGACCG AGCTGGCCGC TGGGAGCCAG GGGACTACTC GCACTGTCTG 
TACACCAATG ACATCACTCG GGTGCTCTAC ACCTTTGTGC TGATGCCCAT CAACGCCTCC 
AATGCATTGA CGTTGGCCCA CCAGCTGCGA GTGTATACCG CAGAGGCCGC CAGCTTCTCA 
GACATGATGG ACGTGGTCTA TGTGGCTCAG ATGATCCAGA AGTTTTTGGG TTACGTTGAC 
CAGATCAAGG AGCTGGTGGA GGTGATGGTG GACATGGCCA GCAACCTGAT GCTGGTGGAT 
GAGCACCTTC TGTGGCTGGC CCAGAGAGAA GACAAAGCCT GCAGTGGCAT TGTGGGTGCC 
CTGGAGCGAA TCGGAGGAGC TGCTCTTAGC CCCCATGCCC AGCACATCTC TGTGAATTCA 
AGAAATGTGG CACTGGAGGC CTACCTCATC AAGCCTCACA GCTACGTGGG TCTGACTTGC 
ACGGCTTTCC AGAGGAGGGA GGTAGGAGTG TCGGGTGCAC AGCCAAGCAG CGTCGGCCAG 
GATGCCCCAG TGGAGCCCGA ACCCCTAGCT GATCAGCAGC TTAGGTTCCG CTGCACCACT 
GGGAGGCCCA ACATTTCTCT GTCATCCTTC CACATCAAGA ATAGCGTGGC CCTGGCCTCC 
ATCCAGCTGC CCCCCAGCCT GTTCTCAACC CTTCCGGCTG CCCTGGCTCC CCCAGTCCCT 
CCAGATTGCA CCCTGCAACT GCTGGTCTTC AGAAATGGCC GTCTTTTCCG CAGCCACGGC 
AACAACACTT CCCGTCCTGG AGCAGCTGGG CCTGGCAAGA GGCGTGGTGT GGCCACCCCA 
GTCATATTTG CAGGAACCAG TGGCTGTGGT GTGGGAAACT TGACGGAGCC CGTGGCTGTG 
TCACTGAGGC ACTGGGCTGA AGGAGCTGAC CCCATGGCAG CTTGGTGGAA CCAGGACGGG 
CCTGGAGGCT GGAGTTCTGA AGGCTGCAGG CTCCGCTACA GCCAGCCCAA CGTCAGCTCC 
CTGTACTGCC AGCACTTGGG CAATGTGGCC GTGCTTATGG AGCTGAATGC ATTCCCGCGG 
GAGGCAGGAG GCTCTGGGGC TGGGTTGCAT CCCGTCGTGT ACCCCTGCAC GGCTTTGCTG 
CTACTCTGTC TCTTCTCCAC CATCATCACG TACATCCTCA ACCACAGCTC CATCCACGTG 
TCCCGGAAGG GTTGGCACAT GCTGCTGAAC CTTTGTTTCC ATATGGCCAT GACCTCTGCT 
GTCTTCGTGG GGGGCGTCAC GCTCACCAAC TACCAAATGG TTTGTCAAGC GGGTATAGCC 
CAGTCAGCAA CTCAGGTCCC AAGCCTCGGT GCCTTGACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509287.3
CDS	329..3013
Translation	MAPRPALGRPGLPLSTPPARPGPSGRRAVRPLRCRSPRGDGLMGAGGRRMPVPPARLLLLPLLPCLLLLAPGTRGAPGCPVPIRGCKCSGERPKGLSGGAHNPARRRVVCGGGDLPEPPDPGLLPNGTITLLLSNNKITGLRNGSFLGLSLLEKLDLRSNVISTVQPGAFLGLGELKRLDLSNNRIGCLTSETFQGLPRLLRLNISGNIYSSLQPGVFDELPALKIVDFGTEFLTCDCRLRWLLPWARNHSLQLSERTLCAYPSALHAHALSSLQESQLRCEGALELHTHYLIPSLRQVVFQGDRLPFQCSASYLGNDTRIHWYHNGAPMESDEQAGIVLAENLIHDCTFITSELTLSHIGVWASGEWECSVSTVQGNTSKKVEIVVLETSASYCPAERVTNNRGDFRWPRTLAGITAYQSCLQYPFTSVPLSGGAPGTRASRRCDRAGRWEPGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDMASNLMLVDEHLLWLAQREDKACSGIVGALERIGGAALSPHAQHISVNSRNVALEAYLIKPHSYVGLTCTAFQRREVGVSGAQPSSVGQDAPVEPEPLADQQLRFRCTTGRPNISLSSFHIKNSVALASIQLPPSLFSTLPAALAPPVPPDCTLQLLVFRNGRLFRSHGNNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSLRHWAEGADPMAAWWNQDGPGGWSSEGCRLRYSQPNVSSLYCQHLGNVAVLMELNAFPREAGGSGAGLHPVVYPCTALLLLCLFSTIITYILNHSSIHVSRKGWHMLLNLCFHMAMTSAVFVGGVTLTNYQMVCQAGIAQSATQVPSLGALTL

Target ORF information:

RefSeq Version	XM_006509224.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor A2 (Adgra2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509224.3

ORF Insert Sequence:

ATGGCCCCCC GCCCCGCCCT CGGGCGCCCG GGACTCCCGC TGAGCACTCC TCCCGCACGC 
CCGGGTCCCT CCGGCCGGCG AGCCGTCCGG CCCCTGCGCT GTAGGTCCCC GCGGGGCGAT 
GGGTTGATGG GCGCGGGGGG ACGCAGGATG CCGGTGCCTC CCGCGCGTTT GCTGCTGCTG 
CCGCTGCTGC CTTGTCTTCT GCTCCTGGCT CCTGGAACTC GGGGTGCGCC TGGCTGCCCG 
GTCCCTATCC GCGGTTGCAA GTGCTCTGGG GAGCGGCCCA AGGGACTAAG TGGCGGCGCC 
CACAACCCGG CTCGAAGGAG GGTGGTGTGC GGCGGTGGGG ATCTCCCCGA ACCTCCAGAT 
CCCGGCCTTC TGCCAAACGG CACCATCACC TTGCTCTTGA GCAACAACAA GATTACTGGG 
CTCCGCAATG GATCCTTCTT GGGACTGTCC CTGTTGGAGA AGTTGGACCT GAGGAGCAAT 
GTCATCAGCA CTGTGCAGCC TGGAGCCTTC CTAGGTCTGG GAGAGCTAAA ACGCTTAGAT 
CTCTCCAACA ATCGGATTGG CTGTCTCACC TCTGAGACAT TTCAAGGGCT CCCTAGACTT 
CTCAGACTAA ACATATCTGG AAACATCTAC TCTAGTCTGC AACCTGGGGT CTTTGATGAG 
CTGCCAGCCC TTAAGATTGT GGACTTTGGT ACTGAGTTTC TGACCTGTGA CTGCCGCCTG 
CGCTGGCTGC TGCCCTGGGC CCGGAATCAC TCCCTGCAGC TGTCTGAGCG CACACTCTGT 
GCCTACCCCA GTGCCCTGCA CGCCCATGCC CTGAGCAGCC TCCAGGAGTC CCAGCTTCGC 
TGTGAAGGGG CCCTGGAACT GCACACCCAC TACCTCATCC CATCCCTTCG CCAAGTGGTG 
TTCCAGGGTG ACCGCCTGCC CTTCCAGTGC TCAGCCAGCT ACTTGGGCAA CGATACCCGG 
ATCCACTGGT ACCACAATGG GGCTCCTATG GAGAGCGATG AGCAGGCCGG CATCGTCCTT 
GCTGAAAACC TTATCCATGA TTGCACCTTC ATCACCAGTG AGCTGACCCT GTCTCACATT 
GGTGTGTGGG CCTCTGGTGA ATGGGAGTGC TCCGTGTCCA CAGTCCAAGG CAACACCAGC 
AAGAAGGTGG AGATAGTAGT GCTGGAGACC TCTGCCTCCT ACTGCCCTGC AGAGCGGGTG 
ACCAACAACC GTGGGGACTT CAGGTGGCCC CGAACCTTGG CTGGCATCAC GGCTTACCAG 
TCCTGTTTAC AGTACCCCTT CACCTCTGTG CCCTTGAGTG GGGGAGCCCC GGGTACCCGA 
GCCTCACGCA GGTGTGACCG AGCTGGCCGC TGGGAGCCAG GGGACTACTC GCACTGTCTG 
TACACCAATG ACATCACTCG GGTGCTCTAC ACCTTTGTGC TGATGCCCAT CAACGCCTCC 
AATGCATTGA CGTTGGCCCA CCAGCTGCGA GTGTATACCG CAGAGGCCGC CAGCTTCTCA 
GACATGATGG ACGTGGTCTA TGTGGCTCAG ATGATCCAGA AGTTTTTGGG TTACGTTGAC 
CAGATCAAGG AGCTGGTGGA GGTGATGGTG GACATGGCCA GCAACCTGAT GCTGGTGGAT 
GAGCACCTTC TGTGGCTGGC CCAGAGAGAA GACAAAGCCT GCAGTGGCAT TGTGGGTGCC 
CTGGAGCGAA TCGGAGGAGC TGCTCTTAGC CCCCATGCCC AGCACATCTC TGTGAATTCA 
AGAAATGTGG CACTGGAGGC CTACCTCATC AAGCCTCACA GCTACGTGGG TCTGACTTGC 
ACGGCTTTCC AGAGGAGGGA GGTAGGAGTG TCGGGTGCAC AGCCAAGCAG CGTCGGCCAG 
GATGCCCCAG TGGAGCCCGA ACCCCTAGCT GATCAGCAGC TTAGGTTCCG CTGCACCACT 
GGGAGGCCCA ACATTTCTCT GTCATCCTTC CACATCAAGA ATAGCGTGGC CCTGGCCTCC 
ATCCAGCTGC CCCCCAGCCT GTTCTCAACC CTTCCGGCTG CCCTGGCTCC CCCAGTCCCT 
CCAGATTGCA CCCTGCAACT GCTGGTCTTC AGAAATGGCC GTCTTTTCCG CAGCCACGGC 
AACAACACTT CCCGTCCTGG AGCAGCTGGG CCTGGCAAGA GGCGTGGTGT GGCCACCCCA 
GTCATATTTG CAGGAACCAG TGGCTGTGGT GTGGGAAACT TGACGGAGCC CGTGGCTGTG 
TCACTGAGGC ACTGGGCTGA AGGAGCTGAC CCCATGGCAG CTTGGTGGAA CCAGGACGGG 
CCTGGAGGCT GGAGTTCTGA AGGCTGCAGG CTCCGCTACA GCCAGCCCAA CGTCAGCTCC 
CTGTACTGCC AGCACTTGGG CAATGTGGCC GTGCTTATGG AGCTGAATGC ATTCCCGCGG 
GAGGCAGGAG GCTCTGGGGC TGGGTTGCAT CCCGTCGTGT ACCCCTGCAC GGCTTTGCTG 
CTACTCTGTC TCTTCTCCAC CATCATCACG TACATCCTCA ACCACAGCTC CATCCACGTG 
TCCCGGAAGG GTTGGCACAT GCTGCTGAAC CTTTGTTTCC ATATGGCCAT GACCTCTGCT 
GTCTTCGTGG GGGGCGTCAC GCTCACCAAC TACCAAATGG TTTGTCAAGC GGGTATAGCC 
CAGTCAGCAA CTCAGGTCCC AAGCCTCGGT GCCTTGACCT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Reck and Gpr124 Are Essential Receptor Cofactors for Wnt7a/Wnt7b-Specific Signaling in Mammalian CNS Angiogenesis and Blood-Brain Barrier Regulation.
Neuron95(5)1056-1073.e5(2017 Aug)
Cho C,Smallwood PM,Nathans J

Gpr124 is essential for blood-brain barrier integrity in central nervous system disease.
Nature medicine23(4)450-460(2017 Apr)
Chang J,Mancuso MR,Maier C,Liang X,Yuki K,Yang L,Kwong JW,Wang J,Rao V,Vallon M,Kosinski C,Zhang JJ,Mah AT,Xu L,Li L,Gholamin S,Reyes TF,Li R,Kuhnert F,Han X,Yuan J,Chiou SH,Brettman AD,Daly L,Corney DC,Cheshier SH,Shortliffe LD,Wu X,Snyder M,Chan P,Giffard RG,Chang HY,Andreasson K,Kuo CJ

Early molecular events during retinoic acid induced differentiation of neuromesodermal progenitors.
Biology open5(12)1821-1833(2016 Dec)
Cunningham TJ,Colas A,Duester G

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Adgra2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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