Rdh12 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rdh12
Entrez Gene ID	77974
Full Name	retinol dehydrogenase 12
General protein information	Preferred Names retinol dehydrogenase 12 Names retinol dehydrogenase 12
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 C3
Summary	The protein encoded by this gene is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. The encoded enzyme also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Defects in the human gene are associated with Leber congenital amaurosis type 13, and Retinitis Pigmentosa 53. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001313971.1	NP_001300900.1	retinol dehydrogenase 12 isoform 2 precursor
NM_030017.4	NP_084293.1	retinol dehydrogenase 12 isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	RDH12	NP_689656.2
Canis lupus familiaris (dog)	RDH12	XP_547866.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007572	XP_308302.4
Eremothecium gossypii	AGOS_AAL089W	NP_982453.1
Rattus norvegicus (Norway rat)	Rdh12	NP_001101507.1
Gallus gallus (chicken)	RDH12	XP_421193.1
Kluyveromyces lactis	KLLA0D06127g	XP_453335.1
Oryza sativa (rice)	Os09g0570300	NP_001063985.1
	RDH12	XP_002805156.1
Bos taurus (cattle)	RDH12	NP_899207.1
Danio rerio (zebrafish)	rdh12	NP_001002325.1
Saccharomyces cerevisiae (baker's yeast)	ENV9	NP_014889.3
Arabidopsis thaliana (thale cress)	AT4G23420	NP_001190811.1
Pan troglodytes (chimpanzee)	RDH12	XP_003314454.1
Mus musculus (house mouse)	Rdh12	NP_084293.1
Schizosaccharomyces pombe (fission yeast)	SPCC736.13	NP_587784.1
Arabidopsis thaliana (thale cress)	AT4G23430	NP_849428.1
Arabidopsis thaliana (thale cress)	AT4G11410	NP_192880.1

Gene Ontology
Bibliography

Related articles in PubMed

Placental PPAR? regulates spatiotemporally diverse genes and a unique metabolic network.
Shalom-Barak T, Zhang X, Chu T, Timothy Schaiff W, Reddy JK, Xu J, Sadovsky Y, Barak Y
Developmental biology372(1)143-55(2012 Dec)

Physiological and genomic consequences of adrenergic deficiency during embryonic/fetal development in mice: impact on retinoic acid metabolism.
Osuala K, Baker CN, Nguyen HL, Martinez C, Weinshenker D, Ebert SN
Physiological genomics44(19)934-47(2012 Oct)

Reduction of all-trans-retinal in vertebrate rod photoreceptors requires the combined action of RDH8 and RDH12.
Chen C, Thompson DA, Koutalos Y
The Journal of biological chemistry287(29)24662-70(2012 Jul)

Retinol dehydrogenases (RDHs) in the visual cycle.
Parker RO, Crouch RK
Experimental eye research91(6)788-92(2010 Dec)

Retinoid-related orphan nuclear receptor RORbeta is an early-acting factor in rod photoreceptor development.
Jia L, Oh EC, Ng L, Srinivas M, Brooks M, Swaroop A, Forrest D
Proceedings of the National Academy of Sciences of the United States of America106(41)17534-9(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RDH12 in inner segments can protect vital cell organelles against aldehyde toxicity caused by an intracellular leak of all-trans-retinal, as well as other aldehydes originating both inside and outside the cell.
Title: Reduction of all-trans-retinal in vertebrate rod photoreceptors requires the combined action of RDH8 and RDH12.

a critical function of RDH12 is the reduction of all-trans-retinal that exceeds the reductive capacity of the photoreceptor outer segments
Title: Rdh12 activity and effects on retinoid processing in the murine retina.

Oxidative stress induced by exposure to constant bright light led to a rapid and significant decrease of RDH12 protein. The low and constant expression of RDH11 suggested a housekeeping function for this enzyme.
Title: Retinol dehydrogenases RDH11 and RDH12 in the mouse retina: expression levels during development and regulation by oxidative stress.

The present findings, together with those of earlier studies showing only minor functional deficits in mice deficient for Rdh5, Rdh8, or Rdh11, suggest that the activity of any one isoform is not rate limiting in the visual response.
Title: Targeted disruption of the murine retinal dehydrogenase gene Rdh12 does not limit visual cycle function.

RDH12 plays a unique, nonredundant role in the photoreceptor inner segments to regulate the flow of retinoids in the eye.
Title: Retinol dehydrogenase (RDH12) protects photoreceptors from light-induced degeneration in mice.

The following Rdh12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rdh12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07823	NM_030017.4 Latest version!	Mus musculus retinol dehydrogenase 12 (Rdh12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu83422	NM_001313971.1 Latest version!	Mus musculus retinol dehydrogenase 12 (Rdh12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rdh12 ( NM_030017.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_084293.1 Mus musculus retinol dehydrogenase 12 (Rdh12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07823
Clone ID Related Accession (Same CDS sequence)	NM_030017.4
Accession Version	NM_030017.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	retinol dehydrogenase 12 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC154585.2, AK039233.1 and AW491970.1. On Sep 17, 2015 this sequence version replaced NM_030017.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK039233.1, AK020927.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTTTA TCTTGGTACT GCTTACGTCC TTCCTCTCCA TCTTGTATCT GACAGCTCCA 
TCCATCAGGA AGTTCTTTGC TGGTGGAGTT TGTACAACAA ATGTCCAGAT CCCAGGGAAG 
GTAGTGGTCA TCACAGGTGC CAACACAGGC ATTGGCAAGG AGACAGCCAG AGAGCTTGCT 
CGAAGAGGAG CACGAGTATA CATTGCTTGC CGAGATGTGC TGAAGGGAGA GTCTGCTGCT 
AGTGAAATCC GAGCAGATAC CAAGAACTCC CAGGTGCTAG TGCGGAAATT GGACCTGTCT 
GACACCAAAT CCATCCGAGC CTTTGCTGAA CGCTTCCTAG CAGAGGAAAA GAAGCTTCAT 
ATTTTGATCA ACAATGCAGG AGTGATGATG TGCCCATATT CTAAGACAAC AGATGGCTTT 
GAGACCCACT TTGGAGTCAA CCACCTGGGA CACTTTCTTC TTACATACCT GCTGTTGGAG 
AGGCTGAAGG AGTCTGCTCC CGCACGGGTG GTCAACCTTT CCTCAATAGC TCACCTGATT 
GGCAAGATCC GTTTCCATGA CCTCCAAGGC CAGAAACGAT ACTGCAGTGC TTTTGCCTAT 
GGCCACAGCA AGCTGGCCAA TCTGCTCTTC ACTCGAGAAC TGGCTAAGCG GCTCCAAGGG 
ACTGGAGTCA CCGCCTATGC GGTTCACCCG GGCGTTGTAT TGTCAGAGAT CACCAGGAAC 
TCCTACCTGC TGTGTTTGCT ATGGCGGCTC TTCTCACCCT TCTTCAAGTC CACTTCTCAG 
GGGGCTCAGA CCAGCCTGCA CTGTGCTCTG GCGGAGGACC TAGAGCCCCT GAGTGGAAAG 
TACTTCAGTG ACTGCAAGAG GATGTGGGTA TCCTCAAGGG CCCGGAACAA GAAAACAGCT 
GAGCGATTGT GGAACGTCAG CTGCGAGCTT CTAGGAATCC AGTGGGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084293.1
CDS	129..1079
Translation	MLFILVLLTSFLSILYLTAPSIRKFFAGGVCTTNVQIPGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAERFLAEEKKLHILINNAGVMMCPYSKTTDGFETHFGVNHLGHFLLTYLLLERLKESAPARVVNLSSIAHLIGKIRFHDLQGQKRYCSAFAYGHSKLANLLFTRELAKRLQGTGVTAYAVHPGVVLSEITRNSYLLCLLWRLFSPFFKSTSQGAQTSLHCALAEDLEPLSGKYFSDCKRMWVSSRARNKKTAERLWNVSCELLGIQWE

Target ORF information:

RefSeq Version	NM_030017.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus retinol dehydrogenase 12 (Rdh12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030017.4

ORF Insert Sequence:

ATGCTGTTTA TCTTGGTACT GCTTACGTCC TTCCTCTCCA TCTTGTATCT GACAGCTCCA 
TCCATCAGGA AGTTCTTTGC TGGTGGAGTT TGTACAACAA ATGTCCAGAT CCCAGGGAAG 
GTAGTGGTCA TCACAGGTGC CAACACAGGC ATTGGCAAGG AGACAGCCAG AGAGCTTGCT 
CGAAGAGGAG CACGAGTATA CATTGCTTGC CGAGATGTGC TGAAGGGAGA GTCTGCTGCT 
AGTGAAATCC GAGCAGATAC CAAGAACTCC CAGGTGCTAG TGCGGAAATT GGACCTGTCT 
GACACCAAAT CCATCCGAGC CTTTGCTGAA CGCTTCCTAG CAGAGGAAAA GAAGCTTCAT 
ATTTTGATCA ACAATGCAGG AGTGATGATG TGCCCATATT CTAAGACAAC AGATGGCTTT 
GAGACCCACT TTGGAGTCAA CCACCTGGGA CACTTTCTTC TTACATACCT GCTGTTGGAG 
AGGCTGAAGG AGTCTGCTCC CGCACGGGTG GTCAACCTTT CCTCAATAGC TCACCTGATT 
GGCAAGATCC GTTTCCATGA CCTCCAAGGC CAGAAACGAT ACTGCAGTGC TTTTGCCTAT 
GGCCACAGCA AGCTGGCCAA TCTGCTCTTC ACTCGAGAAC TGGCTAAGCG GCTCCAAGGG 
ACTGGAGTCA CCGCCTATGC GGTTCACCCG GGCGTTGTAT TGTCAGAGAT CACCAGGAAC 
TCCTACCTGC TGTGTTTGCT ATGGCGGCTC TTCTCACCCT TCTTCAAGTC CACTTCTCAG 
GGGGCTCAGA CCAGCCTGCA CTGTGCTCTG GCGGAGGACC TAGAGCCCCT GAGTGGAAAG 
TACTTCAGTG ACTGCAAGAG GATGTGGGTA TCCTCAAGGG CCCGGAACAA GAAAACAGCT 
GAGCGATTGT GGAACGTCAG CTGCGAGCTT CTAGGAATCC AGTGGGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rdh12 ( NM_001313971.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001300900.1 Mus musculus retinol dehydrogenase 12 (Rdh12), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu83422
Clone ID Related Accession (Same CDS sequence)	NM_001313971.1
Accession Version	NM_001313971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	retinol dehydrogenase 12 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC154585.2 and BC016204.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016204.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTTTA TCTTGGTACT GCTTACGTCC TTCCTCTCCA TCTTGTATCT GACAGCTCCA 
TCCATCAGGA AGTTCTTTGC TGGTGGAGTT TGTACAACAA ATGTCCAGAT CCCAGGGAAG 
GTAGTGGTCA TCACAGGTGC CAACACAGGC ATTGGCAAGG AGACAGCCAG AGAGCTTGCT 
CGAAGAGGAG CACGAGTATA CATTGCTTGC CGAGATGTGC TGAAGGGAGA GTCTGCTGCT 
AGTGAAATCC GAGCAGATAC CAAGAACTCC CAGGTGCTAG TGCGGAAATT GGACCTGTCT 
GACACCAAAT CCATCCGAGC CTTTGCTGAA CGCTTCCTAG CAGGAGTGAT GATGTGCCCA 
TATTCTAAGA CAACAGATGG CTTTGAGACC CACTTTGGAG TCAACCACCT GGGACACTTT 
CTTCTTACAT ACCTGCTGTT GGAGAGGCTG AAGGAGTCTG CTCCCGCACG GGTGGTCAAC 
CTTTCCTCAA TAGCTCACCT GATTGGCAAG ATCCGTTTCC ATGACCTCCA AGGCCAGAAA 
CGATACTGCA GTGCTTTTGC CTATGGCCAC AGCAAGCTGG CCAATCTGCT CTTCACTCGA 
GAACTGGCTA AGCGGCTCCA AGGGACTGGA GTCACCGCCT ATGCGGTTCA CCCGGGCGTT 
GTATTGTCAG AGATCACCAG GAACTCCTAC CTGCTGTGTT TGCTATGGCG GCTCTTCTCA 
CCCTTCTTCA AGTCCACTTC TCAGGGGGCT CAGACCAGCC TGCACTGTGC TCTGGCGGAG 
GACCTAGAGC CCCTGAGTGG AAAGTACTTC AGTGACTGCA AGAGGATGTG GGTATCCTCA 
AGGGCCCGGA ACAAGAAAAC AGCTGAGCGA TTGTGGAACG TCAGCTGCGA GCTTCTAGGA 
ATCCAGTGGG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300900.1
CDS	129..1043
Translation	MLFILVLLTSFLSILYLTAPSIRKFFAGGVCTTNVQIPGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAERFLAGVMMCPYSKTTDGFETHFGVNHLGHFLLTYLLLERLKESAPARVVNLSSIAHLIGKIRFHDLQGQKRYCSAFAYGHSKLANLLFTRELAKRLQGTGVTAYAVHPGVVLSEITRNSYLLCLLWRLFSPFFKSTSQGAQTSLHCALAEDLEPLSGKYFSDCKRMWVSSRARNKKTAERLWNVSCELLGIQWE

Target ORF information:

RefSeq Version	NM_001313971.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus retinol dehydrogenase 12 (Rdh12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313971.1

ORF Insert Sequence:

ATGCTGTTTA TCTTGGTACT GCTTACGTCC TTCCTCTCCA TCTTGTATCT GACAGCTCCA 
TCCATCAGGA AGTTCTTTGC TGGTGGAGTT TGTACAACAA ATGTCCAGAT CCCAGGGAAG 
GTAGTGGTCA TCACAGGTGC CAACACAGGC ATTGGCAAGG AGACAGCCAG AGAGCTTGCT 
CGAAGAGGAG CACGAGTATA CATTGCTTGC CGAGATGTGC TGAAGGGAGA GTCTGCTGCT 
AGTGAAATCC GAGCAGATAC CAAGAACTCC CAGGTGCTAG TGCGGAAATT GGACCTGTCT 
GACACCAAAT CCATCCGAGC CTTTGCTGAA CGCTTCCTAG CAGGAGTGAT GATGTGCCCA 
TATTCTAAGA CAACAGATGG CTTTGAGACC CACTTTGGAG TCAACCACCT GGGACACTTT 
CTTCTTACAT ACCTGCTGTT GGAGAGGCTG AAGGAGTCTG CTCCCGCACG GGTGGTCAAC 
CTTTCCTCAA TAGCTCACCT GATTGGCAAG ATCCGTTTCC ATGACCTCCA AGGCCAGAAA 
CGATACTGCA GTGCTTTTGC CTATGGCCAC AGCAAGCTGG CCAATCTGCT CTTCACTCGA 
GAACTGGCTA AGCGGCTCCA AGGGACTGGA GTCACCGCCT ATGCGGTTCA CCCGGGCGTT 
GTATTGTCAG AGATCACCAG GAACTCCTAC CTGCTGTGTT TGCTATGGCG GCTCTTCTCA 
CCCTTCTTCA AGTCCACTTC TCAGGGGGCT CAGACCAGCC TGCACTGTGC TCTGGCGGAG 
GACCTAGAGC CCCTGAGTGG AAAGTACTTC AGTGACTGCA AGAGGATGTG GGTATCCTCA 
AGGGCCCGGA ACAAGAAAAC AGCTGAGCGA TTGTGGAACG TCAGCTGCGA GCTTCTAGGA 
ATCCAGTGGG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Placental PPAR? regulates spatiotemporally diverse genes and a unique metabolic network.
Developmental biology372(1)143-55(2012 Dec)
Shalom-Barak T,Zhang X,Chu T,Timothy Schaiff W,Reddy JK,Xu J,Sadovsky Y,Barak Y

Physiological and genomic consequences of adrenergic deficiency during embryonic/fetal development in mice: impact on retinoic acid metabolism.
Physiological genomics44(19)934-47(2012 Oct)
Osuala K,Baker CN,Nguyen HL,Martinez C,Weinshenker D,Ebert SN

Reduction of all-trans-retinal in vertebrate rod photoreceptors requires the combined action of RDH8 and RDH12.
The Journal of biological chemistry287(29)24662-70(2012 Jul)
Chen C,Thompson DA,Koutalos Y

Retinol dehydrogenases (RDHs) in the visual cycle.
Experimental eye research91(6)788-92(2010 Dec)
Parker RO,Crouch RK

Retinoid-related orphan nuclear receptor RORbeta is an early-acting factor in rod photoreceptor development.
Proceedings of the National Academy of Sciences of the United States of America106(41)17534-9(2009 Oct)
Jia L,Oh EC,Ng L,Srinivas M,Brooks M,Swaroop A,Forrest D

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Rdh12 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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