Notum cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Notum
Entrez Gene ID	77583
Full Name	notum palmitoleoyl-protein carboxylesterase
Synonyms	5730593N15Rik
General protein information	Preferred Names notum palmitoleoyl-protein carboxylesterase Names palmitoleoyl-protein carboxylesterase NOTUM notum pectinacetylesterase homolog protein notum homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 E2

mRNA and Protein(s)

mRNA	Protein	Name
XM_011249314.2	XP_011247616.1	palmitoleoyl-protein carboxylesterase NOTUM isoform X2
XM_011249315.1	XP_011247617.1	palmitoleoyl-protein carboxylesterase NOTUM isoform X3
XM_006534497.3	XP_006534560.1	palmitoleoyl-protein carboxylesterase NOTUM isoform X4
XM_030246425.1	XP_030102285.1	palmitoleoyl-protein carboxylesterase NOTUM isoform X5
XM_006534497.2	XP_006534560.1	palmitoleoyl-protein carboxylesterase NOTUM isoform X4
NM_175263.4	NP_780472.3	palmitoleoyl-protein carboxylesterase NOTUM precursor
XM_006534494.3	XP_006534557.1	palmitoleoyl-protein carboxylesterase NOTUM isoform X1
XM_006534494.4	XP_006534557.1	palmitoleoyl-protein carboxylesterase NOTUM isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NOTUM	XP_003315832.1
Canis lupus familiaris (dog)	NOTUM	XP_540493.3
Homo sapiens (human)	NOTUM	NP_848588.3
Bos taurus (cattle)	NOTUM	XP_003587522.2
Rattus norvegicus (Norway rat)	Notum	NP_001013997.2
Gallus gallus (chicken)	NOTUM	XP_415640.4
Drosophila melanogaster (fruit fly)	Notum	NP_730096.2
Arabidopsis thaliana (thale cress)	AT5G45280	NP_199341.1
Oryza sativa (rice)	Os04g0602500	NP_001053773.1
	NOTUM	XP_001112829.2
Mus musculus (house mouse)	Notum	NP_780472.3
Arabidopsis thaliana (thale cress)	AT4G19410	NP_567585.1
Xenopus tropicalis (tropical clawed frog)	notum	NP_001120228.2
Danio rerio (zebrafish)	notum1a	NP_001155126.1

Gene Ontology
Bibliography

Related articles in PubMed

Generation of Mice with Hepatocyte-Specific Conditional Deletion of Notum.
Canal F, Charawi S, Grimber G, Houbron C, Drouet V, Colnot S, Terris B, Cavard C, Perret C
PloS one11(3)e0150997(2016)

Novel insights into Notum and glypicans regulation in colorectal cancer.
De Robertis M, Arigoni M, Loiacono L, Riccardo F, Calogero RA, Feodorova Y, Tashkova D, Belovejdov V, Sarafian V, Cavallo F, Signori E
Oncotarget6(38)41237-57(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The findings support a significant role for NOTUM in modulating WNT signaling pathways that have pleiotropic effects on tooth and kidney development.
Title: Dentin Dysplasia in Notum Knockout Mice.

Male mice deficient in Notum specifically in the liver develop metabolic dysfunctions implicating Notum in the development of Type 2 diabetes.
Title: Generation of Mice with Hepatocyte-Specific Conditional Deletion of Notum.

Data show that notum and glypican-1 and glypican-3 gene expression during colorectal cancer (CRC) development and present evidence to suggest them as potential new biomarkers of CRC pathogenesis.
Title: Novel insights into Notum and glypicans regulation in colorectal cancer.

Describes the human ortholog, and analyzes both the human and mouse promoters.
Title: Human homolog of NOTUM, overexpressed in hepatocellular carcinoma, is regulated transcriptionally by beta-catenin/TCF.

The following Notum gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Notum cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu71634	XM_011249315.1 Latest version!	Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu47115	XM_006534497.3 Latest version!	Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu85947	XM_030246425.1 Latest version!	Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu21519	NM_175263.4 Latest version!	Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu21519	XM_011249314.2 Latest version!	Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu47113	XM_006534494.4 Latest version!	Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu47115	XM_006534497.2	Mus musculus notum pectinacetylesterase homolog (Drosophila) (Notum), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OMu47113	XM_006534494.3	Mus musculus notum pectinacetylesterase homolog (Drosophila) (Notum), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Notum ( XM_011249315.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247617.1 Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu71634
Clone ID Related Accession (Same CDS sequence)	XM_011249315.1
Accession Version	XM_011249315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	palmitoleoyl-protein carboxylesterase NOTUM isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGTCT GCGCCTTGAT AGGAAAAGCT GTAACACTGC CTCGGTGGCT CCTGCATCTC 
CTTGCTGCGC TCCTTACTAC TACAGTTTTC TGGGTCTTTA GCCCTGGCAA ATTTCTCCTT 
ATTCAAGTGC CACTGCGTGC TCGAGAGTTC ACTTCTTCCA TAGTTATCAG CACCAGGGTT 
AACGCGGATC TCATGCGGGG CGGGCTGTGG GAATTTTCCA CGGACCACAG GACCCTCCAT 
CCACCCACCC GCTGTCGCCA CCTCTGGGGA ATCGGTGGGG TTTCACCAGC TTGTGCGTTC 
CTTTTGCGAT TGGCTGCGGG CGCCCCCGCG CGGCTGTGCT ACTATTTGAA GGAGTCCAAG 
GGCAGTCGGC GGTGGTTACT CTTTCTGGAA GGCGGCTGGT ATTGCTTCAA CCGGGAGAAC 
TGTGATTCCA GATACAGCAC CATGCGGCGC CTCATGAGCT CCAAAGACTG GCCACACACA 
CGCACAGGTA CTGGTATTCT ATCCTCCCAG CCAGAGGAGA ACCCCCACTG GTGGAATGCC 
AATATGGTCT TCATCCCTTA CTGCTCCAGT GACGTTTGGA GTGGGGCTTC ACCCAAGTCT 
GACAAGAATG AATATGCCTT CATGGGCTCC CTCATCATCC AGGAGGTGGT ACGGGAGCTC 
CTGGGCAAAG GGCTGAGCGG GGCAAAGGTG CTGCTGTTGG CCGGGAGCAG CGCTGGGGGC 
ACCGGGGTGC TGTTGAATGT AGACCGCGTG GCTGAGCTGC TGGAGGAGTT GGGCTACCCG 
TCTATCCAGG TGCGGGGCCT GGCTGACTCA GGCTGGTTCC TGGACAACAA GCAGTACCGG 
CGATCAGACT GCATTGATAC TATCAACTGT GCACCCACAG ACGCCATCCG CCGTGGCATC 
AGGTACTGGA GTGGGATGGT CCCAGAGCGT TGCCAGCGCC AGTTCAAGGA AGGAGAGGAA 
TGGAATTGCT TCTTTGGCTA CAAAGTATAT CCGACTCTGC GCTGTCCGGT GTTCGTGGTA 
CAGTGGCTTT TCGATGAGGC CCAGCTTACC GTGGATAACG TGCATCTCAC CGGACAGCCG 
GTTCAGGAAG GCCAGTGGCT ATACATCCAG AATCTGGGCC GAGAACTGCG TGGTACACTC 
AAGGATGTGC AGGCCAGCTT TGCCCCCGCC TGCCTGTCTC ATGAGATCAT CATACGGAGC 
TATTGGACGG ACGTCCAGGT GAAGGGGACC TCACTGCCCA GGGCACTGCA CTGCTGGGAC 
CGGAGCTTCC ATGACAGCCA TAAGGCCAGC AAAACCCCCA TGAAGGGCTG CCCTTTCCAC 
CTGGTAGACA GCTGCCCCTG GCCCCACTGC AATCCCTCAT GCCCTACCAT CCGGGACCAG 
TTCACAGGGC AAGAGATGAA CGTGGCACAG TTCCTTATGC ATATGGGCTT CGATGTGCAG 
ACGGTGGCTC AGCAGCAGGG GATGGAGCCC AGCAAGCTGC TAGGGATGCT GAGTAATGGG 
AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247617.1
CDS	626..2131
Translation	MLVCALIGKAVTLPRWLLHLLAALLTTTVFWVFSPGKFLLIQVPLRAREFTSSIVISTRVNADLMRGGLWEFSTDHRTLHPPTRCRHLWGIGGVSPACAFLLRLAAGAPARLCYYLKESKGSRRWLLFLEGGWYCFNRENCDSRYSTMRRLMSSKDWPHTRTGTGILSSQPEENPHWWNANMVFIPYCSSDVWSGASPKSDKNEYAFMGSLIIQEVVRELLGKGLSGAKVLLLAGSSAGGTGVLLNVDRVAELLEELGYPSIQVRGLADSGWFLDNKQYRRSDCIDTINCAPTDAIRRGIRYWSGMVPERCQRQFKEGEEWNCFFGYKVYPTLRCPVFVVQWLFDEAQLTVDNVHLTGQPVQEGQWLYIQNLGRELRGTLKDVQASFAPACLSHEIIIRSYWTDVQVKGTSLPRALHCWDRSFHDSHKASKTPMKGCPFHLVDSCPWPHCNPSCPTIRDQFTGQEMNVAQFLMHMGFDVQTVAQQQGMEPSKLLGMLSNGN

Target ORF information:

RefSeq Version	XM_011249315.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249315.1

ORF Insert Sequence:

ATGTTAGTCT GCGCCTTGAT AGGAAAAGCT GTAACACTGC CTCGGTGGCT CCTGCATCTC 
CTTGCTGCGC TCCTTACTAC TACAGTTTTC TGGGTCTTTA GCCCTGGCAA ATTTCTCCTT 
ATTCAAGTGC CACTGCGTGC TCGAGAGTTC ACTTCTTCCA TAGTTATCAG CACCAGGGTT 
AACGCGGATC TCATGCGGGG CGGGCTGTGG GAATTTTCCA CGGACCACAG GACCCTCCAT 
CCACCCACCC GCTGTCGCCA CCTCTGGGGA ATCGGTGGGG TTTCACCAGC TTGTGCGTTC 
CTTTTGCGAT TGGCTGCGGG CGCCCCCGCG CGGCTGTGCT ACTATTTGAA GGAGTCCAAG 
GGCAGTCGGC GGTGGTTACT CTTTCTGGAA GGCGGCTGGT ATTGCTTCAA CCGGGAGAAC 
TGTGATTCCA GATACAGCAC CATGCGGCGC CTCATGAGCT CCAAAGACTG GCCACACACA 
CGCACAGGTA CTGGTATTCT ATCCTCCCAG CCAGAGGAGA ACCCCCACTG GTGGAATGCC 
AATATGGTCT TCATCCCTTA CTGCTCCAGT GACGTTTGGA GTGGGGCTTC ACCCAAGTCT 
GACAAGAATG AATATGCCTT CATGGGCTCC CTCATCATCC AGGAGGTGGT ACGGGAGCTC 
CTGGGCAAAG GGCTGAGCGG GGCAAAGGTG CTGCTGTTGG CCGGGAGCAG CGCTGGGGGC 
ACCGGGGTGC TGTTGAATGT AGACCGCGTG GCTGAGCTGC TGGAGGAGTT GGGCTACCCG 
TCTATCCAGG TGCGGGGCCT GGCTGACTCA GGCTGGTTCC TGGACAACAA GCAGTACCGG 
CGATCAGACT GCATTGATAC TATCAACTGT GCACCCACAG ACGCCATCCG CCGTGGCATC 
AGGTACTGGA GTGGGATGGT CCCAGAGCGT TGCCAGCGCC AGTTCAAGGA AGGAGAGGAA 
TGGAATTGCT TCTTTGGCTA CAAAGTATAT CCGACTCTGC GCTGTCCGGT GTTCGTGGTA 
CAGTGGCTTT TCGATGAGGC CCAGCTTACC GTGGATAACG TGCATCTCAC CGGACAGCCG 
GTTCAGGAAG GCCAGTGGCT ATACATCCAG AATCTGGGCC GAGAACTGCG TGGTACACTC 
AAGGATGTGC AGGCCAGCTT TGCCCCCGCC TGCCTGTCTC ATGAGATCAT CATACGGAGC 
TATTGGACGG ACGTCCAGGT GAAGGGGACC TCACTGCCCA GGGCACTGCA CTGCTGGGAC 
CGGAGCTTCC ATGACAGCCA TAAGGCCAGC AAAACCCCCA TGAAGGGCTG CCCTTTCCAC 
CTGGTAGACA GCTGCCCCTG GCCCCACTGC AATCCCTCAT GCCCTACCAT CCGGGACCAG 
TTCACAGGGC AAGAGATGAA CGTGGCACAG TTCCTTATGC ATATGGGCTT CGATGTGCAG 
ACGGTGGCTC AGCAGCAGGG GATGGAGCCC AGCAAGCTGC TAGGGATGCT GAGTAATGGG 
AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Notum ( XM_006534497.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534560.1 Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu47115
Clone ID Related Accession (Same CDS sequence)	XM_006534497.3 , XM_006534497.2
Accession Version	XM_006534497.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	palmitoleoyl-protein carboxylesterase NOTUM isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006534497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGC CCGGGGCCAG CCCGGCCGCG CTGCCCCCCA CGGAGCCGGG CCGGGAGCGC 
CTCCGGCGGT TGCAACGGGT GGCCACAGGC CGGAGTGACA TTGCCATGGG AGGAGAGGTG 
CGCGTGCTGC TACTGCTGGG CCTGCTGCAT TGGGTTGGGG GCAGCGAGGG CAGGAAGACC 
TGGAGGCGCC GTGGCCAGCA GCCACCCCAG CCGCCGCCCC CGCCGCCGCT CCCGCAGCGG 
GCTGAGGTGG AGCCGGGCGC CGGGCAGCCG GTGGAGAGCT TCCCGCTGGA CTTCACGGCA 
GTGGAGGGCA ACATGGACAG CTTTATGGCG CAAGTCAAGA GCCTGGCACA GTCCCTGTAT 
CCCTGCTCTG CGCAGCAGCT CAATGAGGAC CTGCGCCTTC ACCTCCTGCT GAACACGTCG 
GTGACCTGCA ATGACGGCAG CCCCGCCGGC TACTATTTGA AGGAGTCCAA GGGCAGTCGG 
CGGTGGTTAC TCTTTCTGGA AGGCGGCTGG TATTGCTTCA ACCGGGAGAA CTGTGATTCC 
AGATACAGCA CCATGCGGCG CCTCATGAGC TCCAAAGACT GGCCACACAC ACGCACAGGT 
ACTGGTATTC TATCCTCCCA GCCAGAGGAG AACCCCCACT GGTGGAATGC CAATATGGTC 
TTCATCCCTT ACTGCTCCAG TGACGTTTGG AGTGGGGCTT CACCCAAGTC TGACAAGAAT 
GAATATGCCT TCATGGGCTC CCTCATCATC CAGGAGGTGG TACGGGAGCT CCTGGGCAAA 
GGGCTGAGCG GGGCAAAGGT GCTGCTGTTG GCCGGGAGCA GGTGCGGGGC CTGGCTGACT 
CAGGCTGGTT CCTGGACAAC AAGCAGTACC GGCGATCAGA CTGCATTGAT ACTATCAACT 
GTGCACCCAC AGACGCCATC CGCCGTGGCA TCAGGTACTG GAGTGGGATG GTCCCAGAGC 
GTTGCCAGCG CCAGTTCAAG GAAGGAGAGG AATGGAATTG CTTCTTTGGC TACAAAGTAT 
ATCCGACTCT GCGCTGTCCG GTGTTCGTGG TACAGTGGCT TTTCGATGAG GCCCAGCTTA 
CCGTGGATAA CGTGCATCTC ACCGGACAGC CGGTTCAGGA AGGCCAGTGG CTATACATCC 
AGAATCTGGG CCGAGAACTG CGTGGTACAC TCAAGGATGT GCAGGCCAGC TTTGCCCCCG 
CCTGCCTGTC TCATGAGATC ATCATACGGA GCTATTGGAC GGACGTCCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534560.1
CDS	190..1443
Translation	MELPGASPAALPPTEPGRERLRRLQRVATGRSDIAMGGEVRVLLLLGLLHWVGGSEGRKTWRRRGQQPPQPPPPPPLPQRAEVEPGAGQPVESFPLDFTAVEGNMDSFMAQVKSLAQSLYPCSAQQLNEDLRLHLLLNTSVTCNDGSPAGYYLKESKGSRRWLLFLEGGWYCFNRENCDSRYSTMRRLMSSKDWPHTRTGTGILSSQPEENPHWWNANMVFIPYCSSDVWSGASPKSDKNEYAFMGSLIIQEVVRELLGKGLSGAKVLLLAGSRCGAWLTQAGSWTTSSTGDQTALILSTVHPQTPSAVASGTGVGWSQSVASASSRKERNGIASLATKYIRLCAVRCSWYSGFSMRPSLPWITCISPDSRFRKASGYTSRIWAENCVVHSRMCRPALPPPACLMRSSYGAIGRTSR

Target ORF information:

RefSeq Version	XM_006534497.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534497.3

ORF Insert Sequence:

ATGGAGTTGC CCGGGGCCAG CCCGGCCGCG CTGCCCCCCA CGGAGCCGGG CCGGGAGCGC 
CTCCGGCGGT TGCAACGGGT GGCCACAGGC CGGAGTGACA TTGCCATGGG AGGAGAGGTG 
CGCGTGCTGC TACTGCTGGG CCTGCTGCAT TGGGTTGGGG GCAGCGAGGG CAGGAAGACC 
TGGAGGCGCC GTGGCCAGCA GCCACCCCAG CCGCCGCCCC CGCCGCCGCT CCCGCAGCGG 
GCTGAGGTGG AGCCGGGCGC CGGGCAGCCG GTGGAGAGCT TCCCGCTGGA CTTCACGGCA 
GTGGAGGGCA ACATGGACAG CTTTATGGCG CAAGTCAAGA GCCTGGCACA GTCCCTGTAT 
CCCTGCTCTG CGCAGCAGCT CAATGAGGAC CTGCGCCTTC ACCTCCTGCT GAACACGTCG 
GTGACCTGCA ATGACGGCAG CCCCGCCGGC TACTATTTGA AGGAGTCCAA GGGCAGTCGG 
CGGTGGTTAC TCTTTCTGGA AGGCGGCTGG TATTGCTTCA ACCGGGAGAA CTGTGATTCC 
AGATACAGCA CCATGCGGCG CCTCATGAGC TCCAAAGACT GGCCACACAC ACGCACAGGT 
ACTGGTATTC TATCCTCCCA GCCAGAGGAG AACCCCCACT GGTGGAATGC CAATATGGTC 
TTCATCCCTT ACTGCTCCAG TGACGTTTGG AGTGGGGCTT CACCCAAGTC TGACAAGAAT 
GAATATGCCT TCATGGGCTC CCTCATCATC CAGGAGGTGG TACGGGAGCT CCTGGGCAAA 
GGGCTGAGCG GGGCAAAGGT GCTGCTGTTG GCCGGGAGCA GGTGCGGGGC CTGGCTGACT 
CAGGCTGGTT CCTGGACAAC AAGCAGTACC GGCGATCAGA CTGCATTGAT ACTATCAACT 
GTGCACCCAC AGACGCCATC CGCCGTGGCA TCAGGTACTG GAGTGGGATG GTCCCAGAGC 
GTTGCCAGCG CCAGTTCAAG GAAGGAGAGG AATGGAATTG CTTCTTTGGC TACAAAGTAT 
ATCCGACTCT GCGCTGTCCG GTGTTCGTGG TACAGTGGCT TTTCGATGAG GCCCAGCTTA 
CCGTGGATAA CGTGCATCTC ACCGGACAGC CGGTTCAGGA AGGCCAGTGG CTATACATCC 
AGAATCTGGG CCGAGAACTG CGTGGTACAC TCAAGGATGT GCAGGCCAGC TTTGCCCCCG 
CCTGCCTGTC TCATGAGATC ATCATACGGA GCTATTGGAC GGACGTCCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Notum ( XM_030246425.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102285.1 Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu85947
Clone ID Related Accession (Same CDS sequence)	XM_030246425.1
Accession Version	XM_030246425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	palmitoleoyl-protein carboxylesterase NOTUM isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGAG AGGTGCGCGT GCTGCTACTG CTGGGCCTGC TGCATTGGGT TGGGGGCAGC 
GAGGGCAGGA AGACCTGGAG GCGCCGTGGC CAGCAGCCAC CCCAGCCGCC GCCCCCGCCG 
CCGCTCCCGC AGCGGGCTGA GGTGGAGCCG GGCGCCGGGC AGCCGGTGGA GAGCTTCCCG 
CTGGACTTCA CGGCAGTGGA GGGCAACATG GACAGCTTTA TGGCGCAAGT CAAGAGCCTG 
GCACAGTCCC TGTATCCCTG CTCTGCGCAG CAGCTCAATG AGGACCTGCG CCTTCACCTC 
CTGCTGAACA CGTCGGTGAC CTGCAATGAC GGCAGCCCCG CCGGCTACTA TTTGAAGGAG 
TCCAAGGGCA GTCGGCGGTG GTTACTCTTT CTGGAAGGCG GCTGGTATTG CTTCAACCGG 
GAGAACTGTG ATTCCAGATA CAGCACCATG CGGCGCCTCA TGAGCTCCAA AGACTGGCCA 
CACACACGCA CAGGTACTGG TATTCTATCC TCCCAGCCAG AGGAGAACCC CCACTGGTGG 
AATGCCAATA TGGTCTTCAT CCCTTACTGC TCCAGTGACG TTTGGAGTGG GGCTTCACCC 
AAGTCTGACA AGAATGAATA TGCCTTCATG GGCTCCCTCA TCATCCAGGA GGTGGTACGG 
GAGCTCCTGG GCAAAGGGCT GAGCGGGGCA AAGGTGCTGC TGTTGGCCGG GAGCAGGTGC 
GGGGCCTGGC TGACTCAGGC TGGTTCCTGG ACAACAAGCA GTACCGGCGA TCAGACTGCA 
TTGATACTAT CAACTGTGCA CCCACAGACG CCATCCGCCG TGGCATCAGG TACTGGAGTG 
GGATGGTCCC AGAGCGTTGC CAGCGCCAGT TCAAGGAAGG AGAGGAATGG AATTGCTTCT 
TTGGCTACAA AGTATATCCG ACTCTGCGCT GTCCGGTGTT CGTGGTACAG TGGCTTTTCG 
ATGAGGCCCA GCTTACCGTG GATAACGTGC ATCTCACCGG ACAGCCGGTT CAGGAAGGCC 
AGTGGCTATA CATCCAGAAT CTGGGCCGAG AACTGCGTGG TACACTCAAG GATGTGCAGG 
CCAGCTTTGC CCCCGCCTGC CTGTCTCATG AGATCATCAT ACGGAGCTAT TGGACGGACG 
TCCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102285.1
CDS	717..1865
Translation	MGGEVRVLLLLGLLHWVGGSEGRKTWRRRGQQPPQPPPPPPLPQRAEVEPGAGQPVESFPLDFTAVEGNMDSFMAQVKSLAQSLYPCSAQQLNEDLRLHLLLNTSVTCNDGSPAGYYLKESKGSRRWLLFLEGGWYCFNRENCDSRYSTMRRLMSSKDWPHTRTGTGILSSQPEENPHWWNANMVFIPYCSSDVWSGASPKSDKNEYAFMGSLIIQEVVRELLGKGLSGAKVLLLAGSRCGAWLTQAGSWTTSSTGDQTALILSTVHPQTPSAVASGTGVGWSQSVASASSRKERNGIASLATKYIRLCAVRCSWYSGFSMRPSLPWITCISPDSRFRKASGYTSRIWAENCVVHSRMCRPALPPPACLMRSSYGAIGRTSR

Target ORF information:

RefSeq Version	XM_030246425.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246425.1

ORF Insert Sequence:

ATGGGAGGAG AGGTGCGCGT GCTGCTACTG CTGGGCCTGC TGCATTGGGT TGGGGGCAGC 
GAGGGCAGGA AGACCTGGAG GCGCCGTGGC CAGCAGCCAC CCCAGCCGCC GCCCCCGCCG 
CCGCTCCCGC AGCGGGCTGA GGTGGAGCCG GGCGCCGGGC AGCCGGTGGA GAGCTTCCCG 
CTGGACTTCA CGGCAGTGGA GGGCAACATG GACAGCTTTA TGGCGCAAGT CAAGAGCCTG 
GCACAGTCCC TGTATCCCTG CTCTGCGCAG CAGCTCAATG AGGACCTGCG CCTTCACCTC 
CTGCTGAACA CGTCGGTGAC CTGCAATGAC GGCAGCCCCG CCGGCTACTA TTTGAAGGAG 
TCCAAGGGCA GTCGGCGGTG GTTACTCTTT CTGGAAGGCG GCTGGTATTG CTTCAACCGG 
GAGAACTGTG ATTCCAGATA CAGCACCATG CGGCGCCTCA TGAGCTCCAA AGACTGGCCA 
CACACACGCA CAGGTACTGG TATTCTATCC TCCCAGCCAG AGGAGAACCC CCACTGGTGG 
AATGCCAATA TGGTCTTCAT CCCTTACTGC TCCAGTGACG TTTGGAGTGG GGCTTCACCC 
AAGTCTGACA AGAATGAATA TGCCTTCATG GGCTCCCTCA TCATCCAGGA GGTGGTACGG 
GAGCTCCTGG GCAAAGGGCT GAGCGGGGCA AAGGTGCTGC TGTTGGCCGG GAGCAGGTGC 
GGGGCCTGGC TGACTCAGGC TGGTTCCTGG ACAACAAGCA GTACCGGCGA TCAGACTGCA 
TTGATACTAT CAACTGTGCA CCCACAGACG CCATCCGCCG TGGCATCAGG TACTGGAGTG 
GGATGGTCCC AGAGCGTTGC CAGCGCCAGT TCAAGGAAGG AGAGGAATGG AATTGCTTCT 
TTGGCTACAA AGTATATCCG ACTCTGCGCT GTCCGGTGTT CGTGGTACAG TGGCTTTTCG 
ATGAGGCCCA GCTTACCGTG GATAACGTGC ATCTCACCGG ACAGCCGGTT CAGGAAGGCC 
AGTGGCTATA CATCCAGAAT CTGGGCCGAG AACTGCGTGG TACACTCAAG GATGTGCAGG 
CCAGCTTTGC CCCCGCCTGC CTGTCTCATG AGATCATCAT ACGGAGCTAT TGGACGGACG 
TCCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Notum ( NM_175263.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780472.3 Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), mRNA.

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CloneID	OMu21519
Clone ID Related Accession (Same CDS sequence)	XM_011249314.2 , NM_175263.4
Accession Version	NM_175263.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	palmitoleoyl-protein carboxylesterase NOTUM precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL663030.12, AK019987.1, DN174397.1 and AI849590.1. On Aug 6, 2008 this sequence version replaced NM_175263.3. ##Evidence-Data-START## Transcript exon combination :: AK019987.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGGAGGAG AGGTGCGCGT GCTGCTACTG CTGGGCCTGC TGCATTGGGT TGGGGGCAGC 
GAGGGCAGGA AGACCTGGAG GCGCCGTGGC CAGCAGCCAC CCCAGCCGCC GCCCCCGCCG 
CCGCTCCCGC AGCGGGCTGA GGTGGAGCCG GGCGCCGGGC AGCCGGTGGA GAGCTTCCCG 
CTGGACTTCA CGGCAGTGGA GGGCAACATG GACAGCTTTA TGGCGCAAGT CAAGAGCCTG 
GCACAGTCCC TGTATCCCTG CTCTGCGCAG CAGCTCAATG AGGACCTGCG CCTTCACCTC 
CTGCTGAACA CGTCGGTGAC CTGCAATGAC GGCAGCCCCG CCGGCTACTA TTTGAAGGAG 
TCCAAGGGCA GTCGGCGGTG GTTACTCTTT CTGGAAGGCG GCTGGTATTG CTTCAACCGG 
GAGAACTGTG ATTCCAGATA CAGCACCATG CGGCGCCTCA TGAGCTCCAA AGACTGGCCA 
CACACACGCA CAGGTACTGG TATTCTATCC TCCCAGCCAG AGGAGAACCC CCACTGGTGG 
AATGCCAATA TGGTCTTCAT CCCTTACTGC TCCAGTGACG TTTGGAGTGG GGCTTCACCC 
AAGTCTGACA AGAATGAATA TGCCTTCATG GGCTCCCTCA TCATCCAGGA GGTGGTACGG 
GAGCTCCTGG GCAAAGGGCT GAGCGGGGCA AAGGTGCTGC TGTTGGCCGG GAGCAGCGCT 
GGGGGCACCG GGGTGCTGTT GAATGTAGAC CGCGTGGCTG AGCTGCTGGA GGAGTTGGGC 
TACCCGTCTA TCCAGGTGCG GGGCCTGGCT GACTCAGGCT GGTTCCTGGA CAACAAGCAG 
TACCGGCGAT CAGACTGCAT TGATACTATC AACTGTGCAC CCACAGACGC CATCCGCCGT 
GGCATCAGGT ACTGGAGTGG GATGGTCCCA GAGCGTTGCC AGCGCCAGTT CAAGGAAGGA 
GAGGAATGGA ATTGCTTCTT TGGCTACAAA GTATATCCGA CTCTGCGCTG TCCGGTGTTC 
GTGGTACAGT GGCTTTTCGA TGAGGCCCAG CTTACCGTGG ATAACGTGCA TCTCACCGGA 
CAGCCGGTTC AGGAAGGCCA GTGGCTATAC ATCCAGAATC TGGGCCGAGA ACTGCGTGGT 
ACACTCAAGG ATGTGCAGGC CAGCTTTGCC CCCGCCTGCC TGTCTCATGA GATCATCATA 
CGGAGCTATT GGACGGACGT CCAGGTGAAG GGGACCTCAC TGCCCAGGGC ACTGCACTGC 
TGGGACCGGA GCTTCCATGA CAGCCATAAG GCCAGCAAAA CCCCCATGAA GGGCTGCCCT 
TTCCACCTGG TAGACAGCTG CCCCTGGCCC CACTGCAATC CCTCATGCCC TACCATCCGG 
GACCAGTTCA CAGGGCAAGA GATGAACGTG GCACAGTTCC TTATGCATAT GGGCTTCGAT 
GTGCAGACGG TGGCTCAGCA GCAGGGGATG GAGCCCAGCA AGCTGCTAGG GATGCTGAGT 
AATGGGAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780472.3
CDS	77..1588
Translation	MGGEVRVLLLLGLLHWVGGSEGRKTWRRRGQQPPQPPPPPPLPQRAEVEPGAGQPVESFPLDFTAVEGNMDSFMAQVKSLAQSLYPCSAQQLNEDLRLHLLLNTSVTCNDGSPAGYYLKESKGSRRWLLFLEGGWYCFNRENCDSRYSTMRRLMSSKDWPHTRTGTGILSSQPEENPHWWNANMVFIPYCSSDVWSGASPKSDKNEYAFMGSLIIQEVVRELLGKGLSGAKVLLLAGSSAGGTGVLLNVDRVAELLEELGYPSIQVRGLADSGWFLDNKQYRRSDCIDTINCAPTDAIRRGIRYWSGMVPERCQRQFKEGEEWNCFFGYKVYPTLRCPVFVVQWLFDEAQLTVDNVHLTGQPVQEGQWLYIQNLGRELRGTLKDVQASFAPACLSHEIIIRSYWTDVQVKGTSLPRALHCWDRSFHDSHKASKTPMKGCPFHLVDSCPWPHCNPSCPTIRDQFTGQEMNVAQFLMHMGFDVQTVAQQQGMEPSKLLGMLSNGN

Target ORF information:

RefSeq Version	NM_175263.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175263.4

ORF Insert Sequence:

ATGGGAGGAG AGGTGCGCGT GCTGCTACTG CTGGGCCTGC TGCATTGGGT TGGGGGCAGC 
GAGGGCAGGA AGACCTGGAG GCGCCGTGGC CAGCAGCCAC CCCAGCCGCC GCCCCCGCCG 
CCGCTCCCGC AGCGGGCTGA GGTGGAGCCG GGCGCCGGGC AGCCGGTGGA GAGCTTCCCG 
CTGGACTTCA CGGCAGTGGA GGGCAACATG GACAGCTTTA TGGCGCAAGT CAAGAGCCTG 
GCACAGTCCC TGTATCCCTG CTCTGCGCAG CAGCTCAATG AGGACCTGCG CCTTCACCTC 
CTGCTGAACA CGTCGGTGAC CTGCAATGAC GGCAGCCCCG CCGGCTACTA TTTGAAGGAG 
TCCAAGGGCA GTCGGCGGTG GTTACTCTTT CTGGAAGGCG GCTGGTATTG CTTCAACCGG 
GAGAACTGTG ATTCCAGATA CAGCACCATG CGGCGCCTCA TGAGCTCCAA AGACTGGCCA 
CACACACGCA CAGGTACTGG TATTCTATCC TCCCAGCCAG AGGAGAACCC CCACTGGTGG 
AATGCCAATA TGGTCTTCAT CCCTTACTGC TCCAGTGACG TTTGGAGTGG GGCTTCACCC 
AAGTCTGACA AGAATGAATA TGCCTTCATG GGCTCCCTCA TCATCCAGGA GGTGGTACGG 
GAGCTCCTGG GCAAAGGGCT GAGCGGGGCA AAGGTGCTGC TGTTGGCCGG GAGCAGCGCT 
GGGGGCACCG GGGTGCTGTT GAATGTAGAC CGCGTGGCTG AGCTGCTGGA GGAGTTGGGC 
TACCCGTCTA TCCAGGTGCG GGGCCTGGCT GACTCAGGCT GGTTCCTGGA CAACAAGCAG 
TACCGGCGAT CAGACTGCAT TGATACTATC AACTGTGCAC CCACAGACGC CATCCGCCGT 
GGCATCAGGT ACTGGAGTGG GATGGTCCCA GAGCGTTGCC AGCGCCAGTT CAAGGAAGGA 
GAGGAATGGA ATTGCTTCTT TGGCTACAAA GTATATCCGA CTCTGCGCTG TCCGGTGTTC 
GTGGTACAGT GGCTTTTCGA TGAGGCCCAG CTTACCGTGG ATAACGTGCA TCTCACCGGA 
CAGCCGGTTC AGGAAGGCCA GTGGCTATAC ATCCAGAATC TGGGCCGAGA ACTGCGTGGT 
ACACTCAAGG ATGTGCAGGC CAGCTTTGCC CCCGCCTGCC TGTCTCATGA GATCATCATA 
CGGAGCTATT GGACGGACGT CCAGGTGAAG GGGACCTCAC TGCCCAGGGC ACTGCACTGC 
TGGGACCGGA GCTTCCATGA CAGCCATAAG GCCAGCAAAA CCCCCATGAA GGGCTGCCCT 
TTCCACCTGG TAGACAGCTG CCCCTGGCCC CACTGCAATC CCTCATGCCC TACCATCCGG 
GACCAGTTCA CAGGGCAAGA GATGAACGTG GCACAGTTCC TTATGCATAT GGGCTTCGAT 
GTGCAGACGG TGGCTCAGCA GCAGGGGATG GAGCCCAGCA AGCTGCTAGG GATGCTGAGT 
AATGGGAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Notum ( XM_011249314.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247616.1 Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), transcript variant X2, mRNA.

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CloneID	OMu21519
Clone ID Related Accession (Same CDS sequence)	XM_011249314.2 , NM_175263.4
Accession Version	XM_011249314.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	palmitoleoyl-protein carboxylesterase NOTUM isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249314.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGAG AGGTGCGCGT GCTGCTACTG CTGGGCCTGC TGCATTGGGT TGGGGGCAGC 
GAGGGCAGGA AGACCTGGAG GCGCCGTGGC CAGCAGCCAC CCCAGCCGCC GCCCCCGCCG 
CCGCTCCCGC AGCGGGCTGA GGTGGAGCCG GGCGCCGGGC AGCCGGTGGA GAGCTTCCCG 
CTGGACTTCA CGGCAGTGGA GGGCAACATG GACAGCTTTA TGGCGCAAGT CAAGAGCCTG 
GCACAGTCCC TGTATCCCTG CTCTGCGCAG CAGCTCAATG AGGACCTGCG CCTTCACCTC 
CTGCTGAACA CGTCGGTGAC CTGCAATGAC GGCAGCCCCG CCGGCTACTA TTTGAAGGAG 
TCCAAGGGCA GTCGGCGGTG GTTACTCTTT CTGGAAGGCG GCTGGTATTG CTTCAACCGG 
GAGAACTGTG ATTCCAGATA CAGCACCATG CGGCGCCTCA TGAGCTCCAA AGACTGGCCA 
CACACACGCA CAGGTACTGG TATTCTATCC TCCCAGCCAG AGGAGAACCC CCACTGGTGG 
AATGCCAATA TGGTCTTCAT CCCTTACTGC TCCAGTGACG TTTGGAGTGG GGCTTCACCC 
AAGTCTGACA AGAATGAATA TGCCTTCATG GGCTCCCTCA TCATCCAGGA GGTGGTACGG 
GAGCTCCTGG GCAAAGGGCT GAGCGGGGCA AAGGTGCTGC TGTTGGCCGG GAGCAGCGCT 
GGGGGCACCG GGGTGCTGTT GAATGTAGAC CGCGTGGCTG AGCTGCTGGA GGAGTTGGGC 
TACCCGTCTA TCCAGGTGCG GGGCCTGGCT GACTCAGGCT GGTTCCTGGA CAACAAGCAG 
TACCGGCGAT CAGACTGCAT TGATACTATC AACTGTGCAC CCACAGACGC CATCCGCCGT 
GGCATCAGGT ACTGGAGTGG GATGGTCCCA GAGCGTTGCC AGCGCCAGTT CAAGGAAGGA 
GAGGAATGGA ATTGCTTCTT TGGCTACAAA GTATATCCGA CTCTGCGCTG TCCGGTGTTC 
GTGGTACAGT GGCTTTTCGA TGAGGCCCAG CTTACCGTGG ATAACGTGCA TCTCACCGGA 
CAGCCGGTTC AGGAAGGCCA GTGGCTATAC ATCCAGAATC TGGGCCGAGA ACTGCGTGGT 
ACACTCAAGG ATGTGCAGGC CAGCTTTGCC CCCGCCTGCC TGTCTCATGA GATCATCATA 
CGGAGCTATT GGACGGACGT CCAGGTGAAG GGGACCTCAC TGCCCAGGGC ACTGCACTGC 
TGGGACCGGA GCTTCCATGA CAGCCATAAG GCCAGCAAAA CCCCCATGAA GGGCTGCCCT 
TTCCACCTGG TAGACAGCTG CCCCTGGCCC CACTGCAATC CCTCATGCCC TACCATCCGG 
GACCAGTTCA CAGGGCAAGA GATGAACGTG GCACAGTTCC TTATGCATAT GGGCTTCGAT 
GTGCAGACGG TGGCTCAGCA GCAGGGGATG GAGCCCAGCA AGCTGCTAGG GATGCTGAGT 
AATGGGAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247616.1
CDS	717..2228
Translation	MGGEVRVLLLLGLLHWVGGSEGRKTWRRRGQQPPQPPPPPPLPQRAEVEPGAGQPVESFPLDFTAVEGNMDSFMAQVKSLAQSLYPCSAQQLNEDLRLHLLLNTSVTCNDGSPAGYYLKESKGSRRWLLFLEGGWYCFNRENCDSRYSTMRRLMSSKDWPHTRTGTGILSSQPEENPHWWNANMVFIPYCSSDVWSGASPKSDKNEYAFMGSLIIQEVVRELLGKGLSGAKVLLLAGSSAGGTGVLLNVDRVAELLEELGYPSIQVRGLADSGWFLDNKQYRRSDCIDTINCAPTDAIRRGIRYWSGMVPERCQRQFKEGEEWNCFFGYKVYPTLRCPVFVVQWLFDEAQLTVDNVHLTGQPVQEGQWLYIQNLGRELRGTLKDVQASFAPACLSHEIIIRSYWTDVQVKGTSLPRALHCWDRSFHDSHKASKTPMKGCPFHLVDSCPWPHCNPSCPTIRDQFTGQEMNVAQFLMHMGFDVQTVAQQQGMEPSKLLGMLSNGN

Target ORF information:

RefSeq Version	XM_011249314.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249314.2

ORF Insert Sequence:

ATGGGAGGAG AGGTGCGCGT GCTGCTACTG CTGGGCCTGC TGCATTGGGT TGGGGGCAGC 
GAGGGCAGGA AGACCTGGAG GCGCCGTGGC CAGCAGCCAC CCCAGCCGCC GCCCCCGCCG 
CCGCTCCCGC AGCGGGCTGA GGTGGAGCCG GGCGCCGGGC AGCCGGTGGA GAGCTTCCCG 
CTGGACTTCA CGGCAGTGGA GGGCAACATG GACAGCTTTA TGGCGCAAGT CAAGAGCCTG 
GCACAGTCCC TGTATCCCTG CTCTGCGCAG CAGCTCAATG AGGACCTGCG CCTTCACCTC 
CTGCTGAACA CGTCGGTGAC CTGCAATGAC GGCAGCCCCG CCGGCTACTA TTTGAAGGAG 
TCCAAGGGCA GTCGGCGGTG GTTACTCTTT CTGGAAGGCG GCTGGTATTG CTTCAACCGG 
GAGAACTGTG ATTCCAGATA CAGCACCATG CGGCGCCTCA TGAGCTCCAA AGACTGGCCA 
CACACACGCA CAGGTACTGG TATTCTATCC TCCCAGCCAG AGGAGAACCC CCACTGGTGG 
AATGCCAATA TGGTCTTCAT CCCTTACTGC TCCAGTGACG TTTGGAGTGG GGCTTCACCC 
AAGTCTGACA AGAATGAATA TGCCTTCATG GGCTCCCTCA TCATCCAGGA GGTGGTACGG 
GAGCTCCTGG GCAAAGGGCT GAGCGGGGCA AAGGTGCTGC TGTTGGCCGG GAGCAGCGCT 
GGGGGCACCG GGGTGCTGTT GAATGTAGAC CGCGTGGCTG AGCTGCTGGA GGAGTTGGGC 
TACCCGTCTA TCCAGGTGCG GGGCCTGGCT GACTCAGGCT GGTTCCTGGA CAACAAGCAG 
TACCGGCGAT CAGACTGCAT TGATACTATC AACTGTGCAC CCACAGACGC CATCCGCCGT 
GGCATCAGGT ACTGGAGTGG GATGGTCCCA GAGCGTTGCC AGCGCCAGTT CAAGGAAGGA 
GAGGAATGGA ATTGCTTCTT TGGCTACAAA GTATATCCGA CTCTGCGCTG TCCGGTGTTC 
GTGGTACAGT GGCTTTTCGA TGAGGCCCAG CTTACCGTGG ATAACGTGCA TCTCACCGGA 
CAGCCGGTTC AGGAAGGCCA GTGGCTATAC ATCCAGAATC TGGGCCGAGA ACTGCGTGGT 
ACACTCAAGG ATGTGCAGGC CAGCTTTGCC CCCGCCTGCC TGTCTCATGA GATCATCATA 
CGGAGCTATT GGACGGACGT CCAGGTGAAG GGGACCTCAC TGCCCAGGGC ACTGCACTGC 
TGGGACCGGA GCTTCCATGA CAGCCATAAG GCCAGCAAAA CCCCCATGAA GGGCTGCCCT 
TTCCACCTGG TAGACAGCTG CCCCTGGCCC CACTGCAATC CCTCATGCCC TACCATCCGG 
GACCAGTTCA CAGGGCAAGA GATGAACGTG GCACAGTTCC TTATGCATAT GGGCTTCGAT 
GTGCAGACGG TGGCTCAGCA GCAGGGGATG GAGCCCAGCA AGCTGCTAGG GATGCTGAGT 
AATGGGAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Notum ( XM_006534494.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534557.1 Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), transcript variant X1, mRNA.

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CloneID	OMu47113
Clone ID Related Accession (Same CDS sequence)	XM_006534494.3 , XM_006534494.4
Accession Version	XM_006534494.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	palmitoleoyl-protein carboxylesterase NOTUM isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006534494.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGC CCGGGGCCAG CCCGGCCGCG CTGCCCCCCA CGGAGCCGGG CCGGGAGCGC 
CTCCGGCGGT TGCAACGGGT GGCCACAGGC CGGAGTGACA TTGCCATGGG AGGAGAGGTG 
CGCGTGCTGC TACTGCTGGG CCTGCTGCAT TGGGTTGGGG GCAGCGAGGG CAGGAAGACC 
TGGAGGCGCC GTGGCCAGCA GCCACCCCAG CCGCCGCCCC CGCCGCCGCT CCCGCAGCGG 
GCTGAGGTGG AGCCGGGCGC CGGGCAGCCG GTGGAGAGCT TCCCGCTGGA CTTCACGGCA 
GTGGAGGGCA ACATGGACAG CTTTATGGCG CAAGTCAAGA GCCTGGCACA GTCCCTGTAT 
CCCTGCTCTG CGCAGCAGCT CAATGAGGAC CTGCGCCTTC ACCTCCTGCT GAACACGTCG 
GTGACCTGCA ATGACGGCAG CCCCGCCGGC TACTATTTGA AGGAGTCCAA GGGCAGTCGG 
CGGTGGTTAC TCTTTCTGGA AGGCGGCTGG TATTGCTTCA ACCGGGAGAA CTGTGATTCC 
AGATACAGCA CCATGCGGCG CCTCATGAGC TCCAAAGACT GGCCACACAC ACGCACAGGT 
ACTGGTATTC TATCCTCCCA GCCAGAGGAG AACCCCCACT GGTGGAATGC CAATATGGTC 
TTCATCCCTT ACTGCTCCAG TGACGTTTGG AGTGGGGCTT CACCCAAGTC TGACAAGAAT 
GAATATGCCT TCATGGGCTC CCTCATCATC CAGGAGGTGG TACGGGAGCT CCTGGGCAAA 
GGGCTGAGCG GGGCAAAGGT GCTGCTGTTG GCCGGGAGCA GCGCTGGGGG CACCGGGGTG 
CTGTTGAATG TAGACCGCGT GGCTGAGCTG CTGGAGGAGT TGGGCTACCC GTCTATCCAG 
GTGCGGGGCC TGGCTGACTC AGGCTGGTTC CTGGACAACA AGCAGTACCG GCGATCAGAC 
TGCATTGATA CTATCAACTG TGCACCCACA GACGCCATCC GCCGTGGCAT CAGGTACTGG 
AGTGGGATGG TCCCAGAGCG TTGCCAGCGC CAGTTCAAGG AAGGAGAGGA ATGGAATTGC 
TTCTTTGGCT ACAAAGTATA TCCGACTCTG CGCTGTCCGG TGTTCGTGGT ACAGTGGCTT 
TTCGATGAGG CCCAGCTTAC CGTGGATAAC GTGCATCTCA CCGGACAGCC GGTTCAGGAA 
GGCCAGTGGC TATACATCCA GAATCTGGGC CGAGAACTGC GTGGTACACT CAAGGATGTG 
CAGGCCAGCT TTGCCCCCGC CTGCCTGTCT CATGAGATCA TCATACGGAG CTATTGGACG 
GACGTCCAGG TGAAGGGGAC CTCACTGCCC AGGGCACTGC ACTGCTGGGA CCGGAGCTTC 
CATGACAGCC ATAAGGCCAG CAAAACCCCC ATGAAGGGCT GCCCTTTCCA CCTGGTAGAC 
AGCTGCCCCT GGCCCCACTG CAATCCCTCA TGCCCTACCA TCCGGGACCA GTTCACAGGG 
CAAGAGATGA ACGTGGCACA GTTCCTTATG CATATGGGCT TCGATGTGCA GACGGTGGCT 
CAGCAGCAGG GGATGGAGCC CAGCAAGCTG CTAGGGATGC TGAGTAATGG GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534557.1
CDS	184..1800
Translation	MELPGASPAALPPTEPGRERLRRLQRVATGRSDIAMGGEVRVLLLLGLLHWVGGSEGRKTWRRRGQQPPQPPPPPPLPQRAEVEPGAGQPVESFPLDFTAVEGNMDSFMAQVKSLAQSLYPCSAQQLNEDLRLHLLLNTSVTCNDGSPAGYYLKESKGSRRWLLFLEGGWYCFNRENCDSRYSTMRRLMSSKDWPHTRTGTGILSSQPEENPHWWNANMVFIPYCSSDVWSGASPKSDKNEYAFMGSLIIQEVVRELLGKGLSGAKVLLLAGSSAGGTGVLLNVDRVAELLEELGYPSIQVRGLADSGWFLDNKQYRRSDCIDTINCAPTDAIRRGIRYWSGMVPERCQRQFKEGEEWNCFFGYKVYPTLRCPVFVVQWLFDEAQLTVDNVHLTGQPVQEGQWLYIQNLGRELRGTLKDVQASFAPACLSHEIIIRSYWTDVQVKGTSLPRALHCWDRSFHDSHKASKTPMKGCPFHLVDSCPWPHCNPSCPTIRDQFTGQEMNVAQFLMHMGFDVQTVAQQQGMEPSKLLGMLSNGN

Target ORF information:

RefSeq Version	XM_006534494.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus notum palmitoleoyl-protein carboxylesterase (Notum), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534494.4

ORF Insert Sequence:

ATGGAGTTGC CCGGGGCCAG CCCGGCCGCG CTGCCCCCCA CGGAGCCGGG CCGGGAGCGC 
CTCCGGCGGT TGCAACGGGT GGCCACAGGC CGGAGTGACA TTGCCATGGG AGGAGAGGTG 
CGCGTGCTGC TACTGCTGGG CCTGCTGCAT TGGGTTGGGG GCAGCGAGGG CAGGAAGACC 
TGGAGGCGCC GTGGCCAGCA GCCACCCCAG CCGCCGCCCC CGCCGCCGCT CCCGCAGCGG 
GCTGAGGTGG AGCCGGGCGC CGGGCAGCCG GTGGAGAGCT TCCCGCTGGA CTTCACGGCA 
GTGGAGGGCA ACATGGACAG CTTTATGGCG CAAGTCAAGA GCCTGGCACA GTCCCTGTAT 
CCCTGCTCTG CGCAGCAGCT CAATGAGGAC CTGCGCCTTC ACCTCCTGCT GAACACGTCG 
GTGACCTGCA ATGACGGCAG CCCCGCCGGC TACTATTTGA AGGAGTCCAA GGGCAGTCGG 
CGGTGGTTAC TCTTTCTGGA AGGCGGCTGG TATTGCTTCA ACCGGGAGAA CTGTGATTCC 
AGATACAGCA CCATGCGGCG CCTCATGAGC TCCAAAGACT GGCCACACAC ACGCACAGGT 
ACTGGTATTC TATCCTCCCA GCCAGAGGAG AACCCCCACT GGTGGAATGC CAATATGGTC 
TTCATCCCTT ACTGCTCCAG TGACGTTTGG AGTGGGGCTT CACCCAAGTC TGACAAGAAT 
GAATATGCCT TCATGGGCTC CCTCATCATC CAGGAGGTGG TACGGGAGCT CCTGGGCAAA 
GGGCTGAGCG GGGCAAAGGT GCTGCTGTTG GCCGGGAGCA GCGCTGGGGG CACCGGGGTG 
CTGTTGAATG TAGACCGCGT GGCTGAGCTG CTGGAGGAGT TGGGCTACCC GTCTATCCAG 
GTGCGGGGCC TGGCTGACTC AGGCTGGTTC CTGGACAACA AGCAGTACCG GCGATCAGAC 
TGCATTGATA CTATCAACTG TGCACCCACA GACGCCATCC GCCGTGGCAT CAGGTACTGG 
AGTGGGATGG TCCCAGAGCG TTGCCAGCGC CAGTTCAAGG AAGGAGAGGA ATGGAATTGC 
TTCTTTGGCT ACAAAGTATA TCCGACTCTG CGCTGTCCGG TGTTCGTGGT ACAGTGGCTT 
TTCGATGAGG CCCAGCTTAC CGTGGATAAC GTGCATCTCA CCGGACAGCC GGTTCAGGAA 
GGCCAGTGGC TATACATCCA GAATCTGGGC CGAGAACTGC GTGGTACACT CAAGGATGTG 
CAGGCCAGCT TTGCCCCCGC CTGCCTGTCT CATGAGATCA TCATACGGAG CTATTGGACG 
GACGTCCAGG TGAAGGGGAC CTCACTGCCC AGGGCACTGC ACTGCTGGGA CCGGAGCTTC 
CATGACAGCC ATAAGGCCAG CAAAACCCCC ATGAAGGGCT GCCCTTTCCA CCTGGTAGAC 
AGCTGCCCCT GGCCCCACTG CAATCCCTCA TGCCCTACCA TCCGGGACCA GTTCACAGGG 
CAAGAGATGA ACGTGGCACA GTTCCTTATG CATATGGGCT TCGATGTGCA GACGGTGGCT 
CAGCAGCAGG GGATGGAGCC CAGCAAGCTG CTAGGGATGC TGAGTAATGG GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Notum ( XM_006534494.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534557.1 Mus musculus notum pectinacetylesterase homolog (Drosophila) (Notum), transcript variant X1, mRNA.

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CloneID	OMu47113
Clone ID Related Accession (Same CDS sequence)	XM_006534494.3 , XM_006534494.4
Accession Version	XM_006534494.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	palmitoleoyl-protein carboxylesterase NOTUM isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534494.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGC CCGGGGCCAG CCCGGCCGCG CTGCCCCCCA CGGAGCCGGG CCGGGAGCGC 
CTCCGGCGGT TGCAACGGGT GGCCACAGGC CGGAGTGACA TTGCCATGGG AGGAGAGGTG 
CGCGTGCTGC TACTGCTGGG CCTGCTGCAT TGGGTTGGGG GCAGCGAGGG CAGGAAGACC 
TGGAGGCGCC GTGGCCAGCA GCCACCCCAG CCGCCGCCCC CGCCGCCGCT CCCGCAGCGG 
GCTGAGGTGG AGCCGGGCGC CGGGCAGCCG GTGGAGAGCT TCCCGCTGGA CTTCACGGCA 
GTGGAGGGCA ACATGGACAG CTTTATGGCG CAAGTCAAGA GCCTGGCACA GTCCCTGTAT 
CCCTGCTCTG CGCAGCAGCT CAATGAGGAC CTGCGCCTTC ACCTCCTGCT GAACACGTCG 
GTGACCTGCA ATGACGGCAG CCCCGCCGGC TACTATTTGA AGGAGTCCAA GGGCAGTCGG 
CGGTGGTTAC TCTTTCTGGA AGGCGGCTGG TATTGCTTCA ACCGGGAGAA CTGTGATTCC 
AGATACAGCA CCATGCGGCG CCTCATGAGC TCCAAAGACT GGCCACACAC ACGCACAGGT 
ACTGGTATTC TATCCTCCCA GCCAGAGGAG AACCCCCACT GGTGGAATGC CAATATGGTC 
TTCATCCCTT ACTGCTCCAG TGACGTTTGG AGTGGGGCTT CACCCAAGTC TGACAAGAAT 
GAATATGCCT TCATGGGCTC CCTCATCATC CAGGAGGTGG TACGGGAGCT CCTGGGCAAA 
GGGCTGAGCG GGGCAAAGGT GCTGCTGTTG GCCGGGAGCA GCGCTGGGGG CACCGGGGTG 
CTGTTGAATG TAGACCGCGT GGCTGAGCTG CTGGAGGAGT TGGGCTACCC GTCTATCCAG 
GTGCGGGGCC TGGCTGACTC AGGCTGGTTC CTGGACAACA AGCAGTACCG GCGATCAGAC 
TGCATTGATA CTATCAACTG TGCACCCACA GACGCCATCC GCCGTGGCAT CAGGTACTGG 
AGTGGGATGG TCCCAGAGCG TTGCCAGCGC CAGTTCAAGG AAGGAGAGGA ATGGAATTGC 
TTCTTTGGCT ACAAAGTATA TCCGACTCTG CGCTGTCCGG TGTTCGTGGT ACAGTGGCTT 
TTCGATGAGG CCCAGCTTAC CGTGGATAAC GTGCATCTCA CCGGACAGCC GGTTCAGGAA 
GGCCAGTGGC TATACATCCA GAATCTGGGC CGAGAACTGC GTGGTACACT CAAGGATGTG 
CAGGCCAGCT TTGCCCCCGC CTGCCTGTCT CATGAGATCA TCATACGGAG CTATTGGACG 
GACGTCCAGG TGAAGGGGAC CTCACTGCCC AGGGCACTGC ACTGCTGGGA CCGGAGCTTC 
CATGACAGCC ATAAGGCCAG CAAAACCCCC ATGAAGGGCT GCCCTTTCCA CCTGGTAGAC 
AGCTGCCCCT GGCCCCACTG CAATCCCTCA TGCCCTACCA TCCGGGACCA GTTCACAGGG 
CAAGAGATGA ACGTGGCACA GTTCCTTATG CATATGGGCT TCGATGTGCA GACGGTGGCT 
CAGCAGCAGG GGATGGAGCC CAGCAAGCTG CTAGGGATGC TGAGTAATGG GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534557.1
CDS	227..1843
Translation	MELPGASPAALPPTEPGRERLRRLQRVATGRSDIAMGGEVRVLLLLGLLHWVGGSEGRKTWRRRGQQPPQPPPPPPLPQRAEVEPGAGQPVESFPLDFTAVEGNMDSFMAQVKSLAQSLYPCSAQQLNEDLRLHLLLNTSVTCNDGSPAGYYLKESKGSRRWLLFLEGGWYCFNRENCDSRYSTMRRLMSSKDWPHTRTGTGILSSQPEENPHWWNANMVFIPYCSSDVWSGASPKSDKNEYAFMGSLIIQEVVRELLGKGLSGAKVLLLAGSSAGGTGVLLNVDRVAELLEELGYPSIQVRGLADSGWFLDNKQYRRSDCIDTINCAPTDAIRRGIRYWSGMVPERCQRQFKEGEEWNCFFGYKVYPTLRCPVFVVQWLFDEAQLTVDNVHLTGQPVQEGQWLYIQNLGRELRGTLKDVQASFAPACLSHEIIIRSYWTDVQVKGTSLPRALHCWDRSFHDSHKASKTPMKGCPFHLVDSCPWPHCNPSCPTIRDQFTGQEMNVAQFLMHMGFDVQTVAQQQGMEPSKLLGMLSNGN

Target ORF information:

RefSeq Version	XM_006534494.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus notum pectinacetylesterase homolog (Drosophila) (Notum), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534494.3

ORF Insert Sequence:

ATGGAGTTGC CCGGGGCCAG CCCGGCCGCG CTGCCCCCCA CGGAGCCGGG CCGGGAGCGC 
CTCCGGCGGT TGCAACGGGT GGCCACAGGC CGGAGTGACA TTGCCATGGG AGGAGAGGTG 
CGCGTGCTGC TACTGCTGGG CCTGCTGCAT TGGGTTGGGG GCAGCGAGGG CAGGAAGACC 
TGGAGGCGCC GTGGCCAGCA GCCACCCCAG CCGCCGCCCC CGCCGCCGCT CCCGCAGCGG 
GCTGAGGTGG AGCCGGGCGC CGGGCAGCCG GTGGAGAGCT TCCCGCTGGA CTTCACGGCA 
GTGGAGGGCA ACATGGACAG CTTTATGGCG CAAGTCAAGA GCCTGGCACA GTCCCTGTAT 
CCCTGCTCTG CGCAGCAGCT CAATGAGGAC CTGCGCCTTC ACCTCCTGCT GAACACGTCG 
GTGACCTGCA ATGACGGCAG CCCCGCCGGC TACTATTTGA AGGAGTCCAA GGGCAGTCGG 
CGGTGGTTAC TCTTTCTGGA AGGCGGCTGG TATTGCTTCA ACCGGGAGAA CTGTGATTCC 
AGATACAGCA CCATGCGGCG CCTCATGAGC TCCAAAGACT GGCCACACAC ACGCACAGGT 
ACTGGTATTC TATCCTCCCA GCCAGAGGAG AACCCCCACT GGTGGAATGC CAATATGGTC 
TTCATCCCTT ACTGCTCCAG TGACGTTTGG AGTGGGGCTT CACCCAAGTC TGACAAGAAT 
GAATATGCCT TCATGGGCTC CCTCATCATC CAGGAGGTGG TACGGGAGCT CCTGGGCAAA 
GGGCTGAGCG GGGCAAAGGT GCTGCTGTTG GCCGGGAGCA GCGCTGGGGG CACCGGGGTG 
CTGTTGAATG TAGACCGCGT GGCTGAGCTG CTGGAGGAGT TGGGCTACCC GTCTATCCAG 
GTGCGGGGCC TGGCTGACTC AGGCTGGTTC CTGGACAACA AGCAGTACCG GCGATCAGAC 
TGCATTGATA CTATCAACTG TGCACCCACA GACGCCATCC GCCGTGGCAT CAGGTACTGG 
AGTGGGATGG TCCCAGAGCG TTGCCAGCGC CAGTTCAAGG AAGGAGAGGA ATGGAATTGC 
TTCTTTGGCT ACAAAGTATA TCCGACTCTG CGCTGTCCGG TGTTCGTGGT ACAGTGGCTT 
TTCGATGAGG CCCAGCTTAC CGTGGATAAC GTGCATCTCA CCGGACAGCC GGTTCAGGAA 
GGCCAGTGGC TATACATCCA GAATCTGGGC CGAGAACTGC GTGGTACACT CAAGGATGTG 
CAGGCCAGCT TTGCCCCCGC CTGCCTGTCT CATGAGATCA TCATACGGAG CTATTGGACG 
GACGTCCAGG TGAAGGGGAC CTCACTGCCC AGGGCACTGC ACTGCTGGGA CCGGAGCTTC 
CATGACAGCC ATAAGGCCAG CAAAACCCCC ATGAAGGGCT GCCCTTTCCA CCTGGTAGAC 
AGCTGCCCCT GGCCCCACTG CAATCCCTCA TGCCCTACCA TCCGGGACCA GTTCACAGGG 
CAAGAGATGA ACGTGGCACA GTTCCTTATG CATATGGGCT TCGATGTGCA GACGGTGGCT 
CAGCAGCAGG GGATGGAGCC CAGCAAGCTG CTAGGGATGC TGAGTAATGG GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Notum ( XM_006534497.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534560.1 Mus musculus notum pectinacetylesterase homolog (Drosophila) (Notum), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu47115
Clone ID Related Accession (Same CDS sequence)	XM_006534497.3 , XM_006534497.2
Accession Version	XM_006534497.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	palmitoleoyl-protein carboxylesterase NOTUM isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534497.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGC CCGGGGCCAG CCCGGCCGCG CTGCCCCCCA CGGAGCCGGG CCGGGAGCGC 
CTCCGGCGGT TGCAACGGGT GGCCACAGGC CGGAGTGACA TTGCCATGGG AGGAGAGGTG 
CGCGTGCTGC TACTGCTGGG CCTGCTGCAT TGGGTTGGGG GCAGCGAGGG CAGGAAGACC 
TGGAGGCGCC GTGGCCAGCA GCCACCCCAG CCGCCGCCCC CGCCGCCGCT CCCGCAGCGG 
GCTGAGGTGG AGCCGGGCGC CGGGCAGCCG GTGGAGAGCT TCCCGCTGGA CTTCACGGCA 
GTGGAGGGCA ACATGGACAG CTTTATGGCG CAAGTCAAGA GCCTGGCACA GTCCCTGTAT 
CCCTGCTCTG CGCAGCAGCT CAATGAGGAC CTGCGCCTTC ACCTCCTGCT GAACACGTCG 
GTGACCTGCA ATGACGGCAG CCCCGCCGGC TACTATTTGA AGGAGTCCAA GGGCAGTCGG 
CGGTGGTTAC TCTTTCTGGA AGGCGGCTGG TATTGCTTCA ACCGGGAGAA CTGTGATTCC 
AGATACAGCA CCATGCGGCG CCTCATGAGC TCCAAAGACT GGCCACACAC ACGCACAGGT 
ACTGGTATTC TATCCTCCCA GCCAGAGGAG AACCCCCACT GGTGGAATGC CAATATGGTC 
TTCATCCCTT ACTGCTCCAG TGACGTTTGG AGTGGGGCTT CACCCAAGTC TGACAAGAAT 
GAATATGCCT TCATGGGCTC CCTCATCATC CAGGAGGTGG TACGGGAGCT CCTGGGCAAA 
GGGCTGAGCG GGGCAAAGGT GCTGCTGTTG GCCGGGAGCA GGTGCGGGGC CTGGCTGACT 
CAGGCTGGTT CCTGGACAAC AAGCAGTACC GGCGATCAGA CTGCATTGAT ACTATCAACT 
GTGCACCCAC AGACGCCATC CGCCGTGGCA TCAGGTACTG GAGTGGGATG GTCCCAGAGC 
GTTGCCAGCG CCAGTTCAAG GAAGGAGAGG AATGGAATTG CTTCTTTGGC TACAAAGTAT 
ATCCGACTCT GCGCTGTCCG GTGTTCGTGG TACAGTGGCT TTTCGATGAG GCCCAGCTTA 
CCGTGGATAA CGTGCATCTC ACCGGACAGC CGGTTCAGGA AGGCCAGTGG CTATACATCC 
AGAATCTGGG CCGAGAACTG CGTGGTACAC TCAAGGATGT GCAGGCCAGC TTTGCCCCCG 
CCTGCCTGTC TCATGAGATC ATCATACGGA GCTATTGGAC GGACGTCCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534560.1
CDS	230..1483
Translation	MELPGASPAALPPTEPGRERLRRLQRVATGRSDIAMGGEVRVLLLLGLLHWVGGSEGRKTWRRRGQQPPQPPPPPPLPQRAEVEPGAGQPVESFPLDFTAVEGNMDSFMAQVKSLAQSLYPCSAQQLNEDLRLHLLLNTSVTCNDGSPAGYYLKESKGSRRWLLFLEGGWYCFNRENCDSRYSTMRRLMSSKDWPHTRTGTGILSSQPEENPHWWNANMVFIPYCSSDVWSGASPKSDKNEYAFMGSLIIQEVVRELLGKGLSGAKVLLLAGSRCGAWLTQAGSWTTSSTGDQTALILSTVHPQTPSAVASGTGVGWSQSVASASSRKERNGIASLATKYIRLCAVRCSWYSGFSMRPSLPWITCISPDSRFRKASGYTSRIWAENCVVHSRMCRPALPPPACLMRSSYGAIGRTSR

Target ORF information:

RefSeq Version	XM_006534497.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus notum pectinacetylesterase homolog (Drosophila) (Notum), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534497.2

ORF Insert Sequence:

ATGGAGTTGC CCGGGGCCAG CCCGGCCGCG CTGCCCCCCA CGGAGCCGGG CCGGGAGCGC 
CTCCGGCGGT TGCAACGGGT GGCCACAGGC CGGAGTGACA TTGCCATGGG AGGAGAGGTG 
CGCGTGCTGC TACTGCTGGG CCTGCTGCAT TGGGTTGGGG GCAGCGAGGG CAGGAAGACC 
TGGAGGCGCC GTGGCCAGCA GCCACCCCAG CCGCCGCCCC CGCCGCCGCT CCCGCAGCGG 
GCTGAGGTGG AGCCGGGCGC CGGGCAGCCG GTGGAGAGCT TCCCGCTGGA CTTCACGGCA 
GTGGAGGGCA ACATGGACAG CTTTATGGCG CAAGTCAAGA GCCTGGCACA GTCCCTGTAT 
CCCTGCTCTG CGCAGCAGCT CAATGAGGAC CTGCGCCTTC ACCTCCTGCT GAACACGTCG 
GTGACCTGCA ATGACGGCAG CCCCGCCGGC TACTATTTGA AGGAGTCCAA GGGCAGTCGG 
CGGTGGTTAC TCTTTCTGGA AGGCGGCTGG TATTGCTTCA ACCGGGAGAA CTGTGATTCC 
AGATACAGCA CCATGCGGCG CCTCATGAGC TCCAAAGACT GGCCACACAC ACGCACAGGT 
ACTGGTATTC TATCCTCCCA GCCAGAGGAG AACCCCCACT GGTGGAATGC CAATATGGTC 
TTCATCCCTT ACTGCTCCAG TGACGTTTGG AGTGGGGCTT CACCCAAGTC TGACAAGAAT 
GAATATGCCT TCATGGGCTC CCTCATCATC CAGGAGGTGG TACGGGAGCT CCTGGGCAAA 
GGGCTGAGCG GGGCAAAGGT GCTGCTGTTG GCCGGGAGCA GGTGCGGGGC CTGGCTGACT 
CAGGCTGGTT CCTGGACAAC AAGCAGTACC GGCGATCAGA CTGCATTGAT ACTATCAACT 
GTGCACCCAC AGACGCCATC CGCCGTGGCA TCAGGTACTG GAGTGGGATG GTCCCAGAGC 
GTTGCCAGCG CCAGTTCAAG GAAGGAGAGG AATGGAATTG CTTCTTTGGC TACAAAGTAT 
ATCCGACTCT GCGCTGTCCG GTGTTCGTGG TACAGTGGCT TTTCGATGAG GCCCAGCTTA 
CCGTGGATAA CGTGCATCTC ACCGGACAGC CGGTTCAGGA AGGCCAGTGG CTATACATCC 
AGAATCTGGG CCGAGAACTG CGTGGTACAC TCAAGGATGT GCAGGCCAGC TTTGCCCCCG 
CCTGCCTGTC TCATGAGATC ATCATACGGA GCTATTGGAC GGACGTCCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Generation of Mice with Hepatocyte-Specific Conditional Deletion of Notum.
PloS one11(3)e0150997(2016)
Canal F,Charawi S,Grimber G,Houbron C,Drouet V,Colnot S,Terris B,Cavard C,Perret C

Novel insights into Notum and glypicans regulation in colorectal cancer.
Oncotarget6(38)41237-57(2015 Dec)
De Robertis M,Arigoni M,Loiacono L,Riccardo F,Calogero RA,Feodorova Y,Tashkova D,Belovejdov V,Sarafian V,Cavallo F,Signori E

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Notum cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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